Status
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Gene information
General information
TC001571 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 3572202..3575056
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001571
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02693

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    thorax not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    abdomen segment number increased - Penetrance (percentage of animals/eggs on slide): more than 80%

    Comment: Two posterior termini (marked by urogomphi) are present with the hindgut often protruding. The segments in between have abdominal identity and may be part of two opposing abdomina. Segmentation is generally disturbed.
    urogomphi number increased - Penetrance (percentage of animals/eggs on slide): more than 80%
    abdomen segmentation/division irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    pygopodium mostly not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    hindgut protruding - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    hindgut number increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen dorsal not closed - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection



    pupal molt not completely molted - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection



    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection



    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001571 RA
      MGLIYSSSS IVDNVTNYI SGRKRKYSD SEDDEVNKV 
      IDIALHTPK RKKLVSTAQ YIYQALFEE GKNSDITVC 
      ALGEEFYLH KIYLCQSPY FASMFGGSW LEATKKYIH 
      IDVVDPLIT IDSLKVVFG SLYNCEITL NPLQIVSTL 
      ATATMFQLD GLIEKCTEV MLETINPKS SLEYYNAAC 
      QYGDKKLQD VCFKWFLVN LMTYYFSHP VVNLKSIPI 
      PLMTKLVAH PDLFVMQTE YSIYVLLKY WVYILTHPE 
      MGDETPSSK EVNNFFCSR KNEMPYLLT DEGRKFVPV 
      FQGLRIPNL ISHQMDVEL VLHDNIIPH SWLNPVVLQ 
      QWKQVLRVN QNDDKGPSE VSNELFLET SLRCGRILM 
      VNERHVWRW TGFHFGMDL LMMTDGYTV SVKRNHRSE 
      FEQLLSFQT SRHIAIRVT VVKLNEQRQ IVYSKQSEI 
      LKLSLAKGE EVQIMALDK DLEYPIIIS ANILYTSPD 
      SQEINQEEV VQTVS
    CDS sequence
    • >TC001571 RA
      atggggctg atttactcc agtagtagc atagtggac 
      aacgtaacg aattacatt tctgggagg aagcgaaaa 
      tattcggat tcggaagac gatgaagtt aacaaagtc 
      atagacatt gctttacac acaccaaaa aggaaaaag 
      ctagtgagc actgcacag tacatttat caggcgctg 
      tttgaagag gggaagaat tcggacata acagtatgt 
      gccttggga gaagagttc tatttacac aagatttac 
      ttatgtcag agcccctat tttgcgagc atgtttggg 
      ggctcgtgg ctggaagct accaaaaag tatattcat 
      atcgatgta gtggatcca cttattaca atcgactca 
      ctgaaagtg gtatttggg tccttgtac aactgtgag 
      ataactctt aatccatta caaatcgtt tcaacgctg 
      gcgacagcc accatgttc caactcgat ggattaatt 
      gaaaaatgt acggaagtg atgttagaa acaataaat 
      ccgaagtct tctttggag tactataat gcggcatgt 
      cagtacggt gataagaaa ttgcaagac gtgtgcttc 
      aaatggttt ttagtaaat ttaatgacg tattacttt 
      agtcatcct gtagttaat ttaaaaagc attcctata 
      cctcttatg acgaagctt gtagcacat cccgattta 
      tttgtgatg cagactgaa tattccatt tatgtatta 
      ctcaagtat tgggtttat atacttaca catccggaa 
      atgggcgac gagactccg agtagtaaa gaagtgaac 
      aattttttc tgttcgaga aaaaacgag atgccatat 
      cttttgact gacgaggga cgaaaattt gtgccagtt 
      ttccaaggt cttcgaata ccaaactta atttcgcac 
      caaatggac gtagagtta gttcttcac gataatatc 
      ataccgcac agttggctg aatccggtg gttttgcag 
      cagtggaag caggtcttg agagtcaac caaaacgac 
      gataagggc cccagcgaa gtctcaaac gagttattc 
      ctggagact agtctcagg tgtggcagg attttgatg 
      gtgaacgag cgacatgtg tggaggtgg accggattc 
      catttcgga atggattta ctcatgatg accgatggc 
      tacacggtc tctgtcaaa agaaaccac aggtcggag 
      ttcgagcaa ctgttaagc ttccaaacc tcgagacat 
      atagctata agggtaacc gttgtcaaa ttgaacgag 
      caaagacag atagtttac tccaagcag tcagagatc 
      ctaaaactg agccttgcc aaaggggag gaggtgcag 
      ataatggcg cttgacaaa gacttggag tatcctatc 
      atcatatcg gctaatata ctgtacact tcccccgac 
      tcacaggaa ataaatcag gaagaggtc gtgcaaact 
      gtctcctaa
    mRNA sequence
    • >TC001571 RA
      cggccgata aagcaggga gcgaccgcg ccgttgatc 
      agtggtgtt gcagttgta aagtgtcat cattggggc 
      aatatttgt gttaattgg tacataata gtgcaataa 
      aacccaaat ttgggtttg gaattgtcg tagtgactc 
      gttgtgata acgaaaagt tgtccagtt gagccacag 
      tagagtttg cgttgtttc cgtgtcgct cgacgagtc 
      gcacatgtt ataaacaat tacaaaaaa gagaaagtt 
      ttctgcaca tttattatt gttggagag cgtcgtggg 
      ttgacagtg atgtacctt ttcaagttt ttttgaccg 
      ttttttgtg tgttgaact gactgcaat ggaggtagt 
      aaccgaaat ttagtgttt gcggttatt acgtcgcac 
      catggggct gatttactc cagtagtag catagtgga 
      caacgtaac gaattacat ttctgggag gaagcgaaa 
      atattcgga ttcggaaga cgatgaagt taacaaagt 
      catagacat tgctttaca cacaccaaa aaggaaaaa 
      gctagtgag cactgcaca gtacattta tcaggcgct 
      gtttgaaga ggggaagaa ttcggacat aacagtatg 
      tgccttggg agaagagtt ctatttaca caagattta 
      cttatgtca gagccccta ttttgcgag catgtttgg 
      gggctcgtg gctggaagc taccaaaaa gtatattca 
      tatcgatgt agtggatcc acttattac aatcgactc 
      actgaaagt ggtatttgg gtccttgta caactgtga 
      gataactct taatccatt acaaatcgt ttcaacgct 
      ggcgacagc caccatgtt ccaactcga tggattaat 
      tgaaaaatg tacggaagt gatgttaga aacaataaa 
      tccgaagtc ttctttgga gtactataa tgcggcatg 
      tcagtacgg tgataagaa attgcaaga cgtgtgctt 
      caaatggtt tttagtaaa tttaatgac gtattactt 
      tagtcatcc tgtagttaa tttaaaaag cattcctat 
      acctcttat gacgaagct tgtagcaca tcccgattt 
      atttgtgat gcagactga atattccat ttatgtatt 
      actcaagta ttgggttta tatacttac acatccgga 
      aatgggcga cgagactcc gagtagtaa agaagtgaa 
      caatttttt ctgttcgag aaaaaacga gatgccata 
      tcttttgac tgacgaggg acgaaaatt tgtgccagt 
      tttccaagg tcttcgaat accaaactt aatttcgca 
      ccaaatgga cgtagagtt agttcttca cgataatat 
      cataccgca cagttggct gaatccggt ggttttgca 
      gcagtggaa gcaggtctt gagagtcaa ccaaaacga 
      cgataaggg ccccagcga agtctcaaa cgagttatt 
      cctggagac tagtctcag gtgtggcag gattttgat 
      ggtgaacga gcgacatgt gtggaggtg gaccggatt 
      ccatttcgg aatggattt actcatgat gaccgatgg 
      ctacacggt ctctgtcaa aagaaacca caggtcgga 
      gttcgagca actgttaag cttccaaac ctcgagaca 
      tatagctat aagggtaac cgttgtcaa attgaacga 
      gcaaagaca gatagttta ctccaagca gtcagagat 
      cctaaaact gagccttgc caaagggga ggaggtgca 
      gataatggc gcttgacaa agacttgga gtatcctat 
      catcatatc ggctaatat actgtacac ttcccccga 
      ctcacagga aataaatca ggaagaggt cgtgcaaac 
      tgtctccta acgtggtta tttattttg cgtctgcat 
      ttatttgtt atgtacagt atttgttac gtagtgggt 
      tagtaggta tggagagat tttgtgttc ttgcatgag 
      ggtgagttc agtaattcc gtacaaatt tattcacaa 
      atcaaactt taatatcaa aatattgca atatttttg 
      atattattg tttacagtt gatgtgttt tgtgattgc 
      taacaaaaa tatcaaatt cgatttact aatttaata 
      ctgttattt ttttaaacg aggagatta aattaggta 
      ttctaggcg tagtaccta tttaggatt atttaaaag 
      ctttataac agttaccat ttgttaatt ttctaagta 
      aaatattta acaaacttt ctacaccta attttaaga 
      tgtgaaagt gctcttatt aacacctag tcactacgc 
      ttgtcatct aaataaacg gtggattat aacaatttg 
      tcttaatca actgaaccg aacaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY