Status
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Gene information
General information
TC001571 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 3572202..3575056
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC001571
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02693

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     head not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    thorax not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    abdomen segment number increased - Penetrance (percentage of animals/eggs on slide): more than 80%

    Comment: Two posterior termini (marked by urogomphi) are present with the hindgut often protruding. The segments in between have abdominal identity and may be part of two opposing abdomina. Segmentation is generally disturbed.
    urogomphi number increased - Penetrance (percentage of animals/eggs on slide): more than 80%
    abdomen segmentation/division irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    pygopodium mostly not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    hindgut protruding - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    hindgut number increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen dorsal not closed - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not completely molted - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection

    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001571 RA
      MGLIYSSSS IVDNVTNYI SGRKRKYSD SEDDEVNKV 
IDIALHTPK RKKLVSTAQ YIYQALFEE GKNSDITVC 
ALGEEFYLH KIYLCQSPY FASMFGGSW LEATKKYIH 
IDVVDPLIT IDSLKVVFG SLYNCEITL NPLQIVSTL 
ATATMFQLD GLIEKCTEV MLETINPKS SLEYYNAAC 
QYGDKKLQD VCFKWFLVN LMTYYFSHP VVNLKSIPI 
PLMTKLVAH PDLFVMQTE YSIYVLLKY WVYILTHPE 
MGDETPSSK EVNNFFCSR KNEMPYLLT DEGRKFVPV 
FQGLRIPNL ISHQMDVEL VLHDNIIPH SWLNPVVLQ 
QWKQVLRVN QNDDKGPSE VSNELFLET SLRCGRILM 
VNERHVWRW TGFHFGMDL LMMTDGYTV SVKRNHRSE 
FEQLLSFQT SRHIAIRVT VVKLNEQRQ IVYSKQSEI 
LKLSLAKGE EVQIMALDK DLEYPIIIS ANILYTSPD 
SQEINQEEV VQTVS
    CDS sequence
    • >TC001571 RA
      atggggctg atttactcc agtagtagc atagtggac 
aacgtaacg aattacatt tctgggagg aagcgaaaa 
tattcggat tcggaagac gatgaagtt aacaaagtc 
atagacatt gctttacac acaccaaaa aggaaaaag 
ctagtgagc actgcacag tacatttat caggcgctg 
tttgaagag gggaagaat tcggacata acagtatgt 
gccttggga gaagagttc tatttacac aagatttac 
ttatgtcag agcccctat tttgcgagc atgtttggg 
ggctcgtgg ctggaagct accaaaaag tatattcat 
atcgatgta gtggatcca cttattaca atcgactca 
ctgaaagtg gtatttggg tccttgtac aactgtgag 
ataactctt aatccatta caaatcgtt tcaacgctg 
gcgacagcc accatgttc caactcgat ggattaatt 
gaaaaatgt acggaagtg atgttagaa acaataaat 
ccgaagtct tctttggag tactataat gcggcatgt 
cagtacggt gataagaaa ttgcaagac gtgtgcttc 
aaatggttt ttagtaaat ttaatgacg tattacttt 
agtcatcct gtagttaat ttaaaaagc attcctata 
cctcttatg acgaagctt gtagcacat cccgattta 
tttgtgatg cagactgaa tattccatt tatgtatta 
ctcaagtat tgggtttat atacttaca catccggaa 
atgggcgac gagactccg agtagtaaa gaagtgaac 
aattttttc tgttcgaga aaaaacgag atgccatat 
cttttgact gacgaggga cgaaaattt gtgccagtt 
ttccaaggt cttcgaata ccaaactta atttcgcac 
caaatggac gtagagtta gttcttcac gataatatc 
ataccgcac agttggctg aatccggtg gttttgcag 
cagtggaag caggtcttg agagtcaac caaaacgac 
gataagggc cccagcgaa gtctcaaac gagttattc 
ctggagact agtctcagg tgtggcagg attttgatg 
gtgaacgag cgacatgtg tggaggtgg accggattc 
catttcgga atggattta ctcatgatg accgatggc 
tacacggtc tctgtcaaa agaaaccac aggtcggag 
ttcgagcaa ctgttaagc ttccaaacc tcgagacat 
atagctata agggtaacc gttgtcaaa ttgaacgag 
caaagacag atagtttac tccaagcag tcagagatc 
ctaaaactg agccttgcc aaaggggag gaggtgcag 
ataatggcg cttgacaaa gacttggag tatcctatc 
atcatatcg gctaatata ctgtacact tcccccgac 
tcacaggaa ataaatcag gaagaggtc gtgcaaact 
gtctcctaa
    mRNA sequence
    • >TC001571 RA
      cggccgata aagcaggga gcgaccgcg ccgttgatc 
agtggtgtt gcagttgta aagtgtcat cattggggc 
aatatttgt gttaattgg tacataata gtgcaataa 
aacccaaat ttgggtttg gaattgtcg tagtgactc 
gttgtgata acgaaaagt tgtccagtt gagccacag 
tagagtttg cgttgtttc cgtgtcgct cgacgagtc 
gcacatgtt ataaacaat tacaaaaaa gagaaagtt 
ttctgcaca tttattatt gttggagag cgtcgtggg 
ttgacagtg atgtacctt ttcaagttt ttttgaccg 
ttttttgtg tgttgaact gactgcaat ggaggtagt 
aaccgaaat ttagtgttt gcggttatt acgtcgcac 
catggggct gatttactc cagtagtag catagtgga 
caacgtaac gaattacat ttctgggag gaagcgaaa 
atattcgga ttcggaaga cgatgaagt taacaaagt 
catagacat tgctttaca cacaccaaa aaggaaaaa 
gctagtgag cactgcaca gtacattta tcaggcgct 
gtttgaaga ggggaagaa ttcggacat aacagtatg 
tgccttggg agaagagtt ctatttaca caagattta 
cttatgtca gagccccta ttttgcgag catgtttgg 
gggctcgtg gctggaagc taccaaaaa gtatattca 
tatcgatgt agtggatcc acttattac aatcgactc 
actgaaagt ggtatttgg gtccttgta caactgtga 
gataactct taatccatt acaaatcgt ttcaacgct 
ggcgacagc caccatgtt ccaactcga tggattaat 
tgaaaaatg tacggaagt gatgttaga aacaataaa 
tccgaagtc ttctttgga gtactataa tgcggcatg 
tcagtacgg tgataagaa attgcaaga cgtgtgctt 
caaatggtt tttagtaaa tttaatgac gtattactt 
tagtcatcc tgtagttaa tttaaaaag cattcctat 
acctcttat gacgaagct tgtagcaca tcccgattt 
atttgtgat gcagactga atattccat ttatgtatt 
actcaagta ttgggttta tatacttac acatccgga 
aatgggcga cgagactcc gagtagtaa agaagtgaa 
caatttttt ctgttcgag aaaaaacga gatgccata 
tcttttgac tgacgaggg acgaaaatt tgtgccagt 
tttccaagg tcttcgaat accaaactt aatttcgca 
ccaaatgga cgtagagtt agttcttca cgataatat 
cataccgca cagttggct gaatccggt ggttttgca 
gcagtggaa gcaggtctt gagagtcaa ccaaaacga 
cgataaggg ccccagcga agtctcaaa cgagttatt 
cctggagac tagtctcag gtgtggcag gattttgat 
ggtgaacga gcgacatgt gtggaggtg gaccggatt 
ccatttcgg aatggattt actcatgat gaccgatgg 
ctacacggt ctctgtcaa aagaaacca caggtcgga 
gttcgagca actgttaag cttccaaac ctcgagaca 
tatagctat aagggtaac cgttgtcaa attgaacga 
gcaaagaca gatagttta ctccaagca gtcagagat 
cctaaaact gagccttgc caaagggga ggaggtgca 
gataatggc gcttgacaa agacttgga gtatcctat 
catcatatc ggctaatat actgtacac ttcccccga 
ctcacagga aataaatca ggaagaggt cgtgcaaac 
tgtctccta acgtggtta tttattttg cgtctgcat 
ttatttgtt atgtacagt atttgttac gtagtgggt 
tagtaggta tggagagat tttgtgttc ttgcatgag 
ggtgagttc agtaattcc gtacaaatt tattcacaa 
atcaaactt taatatcaa aatattgca atatttttg 
atattattg tttacagtt gatgtgttt tgtgattgc 
taacaaaaa tatcaaatt cgatttact aatttaata 
ctgttattt ttttaaacg aggagatta aattaggta 
ttctaggcg tagtaccta tttaggatt atttaaaag 
ctttataac agttaccat ttgttaatt ttctaagta 
aaatattta acaaacttt ctacaccta attttaaga 
tgtgaaagt gctcttatt aacacctag tcactacgc 
ttgtcatct aaataaacg gtggattat aacaatttg 
tcttaatca actgaaccg aacaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY