General information | |||
TC001571 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 3572202..3575056 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC001571 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_02693 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
remarks: muscle pattern potentially obscured by segmentation defect,
head not present - Penetrance (percentage of animals/eggs on slide): more than 80%
thorax not present - Penetrance (percentage of animals/eggs on slide): more than 80%
abdomen segment number increased - Penetrance (percentage of animals/eggs on slide): more than 80%
Comment: Two posterior termini (marked by urogomphi) are present with the hindgut often protruding. The segments in between have abdominal identity and may be part of two opposing abdomina. Segmentation is generally disturbed. |
abdomen segmentation/division irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
pygopodium mostly not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
hindgut protruding - Penetrance (percentage of animals/eggs on slide): 30% - 50%
hindgut number increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen dorsal not closed - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)
pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 1 (11 dpi)
pupal molt not completely molted - Number of animals showing the phenotype: 2 (11 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (16 dpi)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (16 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (19 dpi)
adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 2 (22 dpi)
- >TC001571 RA
MGLIYSSSS IVDNVTNYI SGRKRKYSD SEDDEVNKV IDIALHTPK RKKLVSTAQ YIYQALFEE GKNSDITVC ALGEEFYLH KIYLCQSPY FASMFGGSW LEATKKYIH IDVVDPLIT IDSLKVVFG SLYNCEITL NPLQIVSTL ATATMFQLD GLIEKCTEV MLETINPKS SLEYYNAAC QYGDKKLQD VCFKWFLVN LMTYYFSHP VVNLKSIPI PLMTKLVAH PDLFVMQTE YSIYVLLKY WVYILTHPE MGDETPSSK EVNNFFCSR KNEMPYLLT DEGRKFVPV FQGLRIPNL ISHQMDVEL VLHDNIIPH SWLNPVVLQ QWKQVLRVN QNDDKGPSE VSNELFLET SLRCGRILM VNERHVWRW TGFHFGMDL LMMTDGYTV SVKRNHRSE FEQLLSFQT SRHIAIRVT VVKLNEQRQ IVYSKQSEI LKLSLAKGE EVQIMALDK DLEYPIIIS ANILYTSPD SQEINQEEV VQTVS
- >TC001571 RA
atggggctg atttactcc agtagtagc atagtggac aacgtaacg aattacatt tctgggagg aagcgaaaa tattcggat tcggaagac gatgaagtt aacaaagtc atagacatt gctttacac acaccaaaa aggaaaaag ctagtgagc actgcacag tacatttat caggcgctg tttgaagag gggaagaat tcggacata acagtatgt gccttggga gaagagttc tatttacac aagatttac ttatgtcag agcccctat tttgcgagc atgtttggg ggctcgtgg ctggaagct accaaaaag tatattcat atcgatgta gtggatcca cttattaca atcgactca ctgaaagtg gtatttggg tccttgtac aactgtgag ataactctt aatccatta caaatcgtt tcaacgctg gcgacagcc accatgttc caactcgat ggattaatt gaaaaatgt acggaagtg atgttagaa acaataaat ccgaagtct tctttggag tactataat gcggcatgt cagtacggt gataagaaa ttgcaagac gtgtgcttc aaatggttt ttagtaaat ttaatgacg tattacttt agtcatcct gtagttaat ttaaaaagc attcctata cctcttatg acgaagctt gtagcacat cccgattta tttgtgatg cagactgaa tattccatt tatgtatta ctcaagtat tgggtttat atacttaca catccggaa atgggcgac gagactccg agtagtaaa gaagtgaac aattttttc tgttcgaga aaaaacgag atgccatat cttttgact gacgaggga cgaaaattt gtgccagtt ttccaaggt cttcgaata ccaaactta atttcgcac caaatggac gtagagtta gttcttcac gataatatc ataccgcac agttggctg aatccggtg gttttgcag cagtggaag caggtcttg agagtcaac caaaacgac gataagggc cccagcgaa gtctcaaac gagttattc ctggagact agtctcagg tgtggcagg attttgatg gtgaacgag cgacatgtg tggaggtgg accggattc catttcgga atggattta ctcatgatg accgatggc tacacggtc tctgtcaaa agaaaccac aggtcggag ttcgagcaa ctgttaagc ttccaaacc tcgagacat atagctata agggtaacc gttgtcaaa ttgaacgag caaagacag atagtttac tccaagcag tcagagatc ctaaaactg agccttgcc aaaggggag gaggtgcag ataatggcg cttgacaaa gacttggag tatcctatc atcatatcg gctaatata ctgtacact tcccccgac tcacaggaa ataaatcag gaagaggtc gtgcaaact gtctcctaa
- >TC001571 RA
cggccgata aagcaggga gcgaccgcg ccgttgatc agtggtgtt gcagttgta aagtgtcat cattggggc aatatttgt gttaattgg tacataata gtgcaataa aacccaaat ttgggtttg gaattgtcg tagtgactc gttgtgata acgaaaagt tgtccagtt gagccacag tagagtttg cgttgtttc cgtgtcgct cgacgagtc gcacatgtt ataaacaat tacaaaaaa gagaaagtt ttctgcaca tttattatt gttggagag cgtcgtggg ttgacagtg atgtacctt ttcaagttt ttttgaccg ttttttgtg tgttgaact gactgcaat ggaggtagt aaccgaaat ttagtgttt gcggttatt acgtcgcac catggggct gatttactc cagtagtag catagtgga caacgtaac gaattacat ttctgggag gaagcgaaa atattcgga ttcggaaga cgatgaagt taacaaagt catagacat tgctttaca cacaccaaa aaggaaaaa gctagtgag cactgcaca gtacattta tcaggcgct gtttgaaga ggggaagaa ttcggacat aacagtatg tgccttggg agaagagtt ctatttaca caagattta cttatgtca gagccccta ttttgcgag catgtttgg gggctcgtg gctggaagc taccaaaaa gtatattca tatcgatgt agtggatcc acttattac aatcgactc actgaaagt ggtatttgg gtccttgta caactgtga gataactct taatccatt acaaatcgt ttcaacgct ggcgacagc caccatgtt ccaactcga tggattaat tgaaaaatg tacggaagt gatgttaga aacaataaa tccgaagtc ttctttgga gtactataa tgcggcatg tcagtacgg tgataagaa attgcaaga cgtgtgctt caaatggtt tttagtaaa tttaatgac gtattactt tagtcatcc tgtagttaa tttaaaaag cattcctat acctcttat gacgaagct tgtagcaca tcccgattt atttgtgat gcagactga atattccat ttatgtatt actcaagta ttgggttta tatacttac acatccgga aatgggcga cgagactcc gagtagtaa agaagtgaa caatttttt ctgttcgag aaaaaacga gatgccata tcttttgac tgacgaggg acgaaaatt tgtgccagt tttccaagg tcttcgaat accaaactt aatttcgca ccaaatgga cgtagagtt agttcttca cgataatat cataccgca cagttggct gaatccggt ggttttgca gcagtggaa gcaggtctt gagagtcaa ccaaaacga cgataaggg ccccagcga agtctcaaa cgagttatt cctggagac tagtctcag gtgtggcag gattttgat ggtgaacga gcgacatgt gtggaggtg gaccggatt ccatttcgg aatggattt actcatgat gaccgatgg ctacacggt ctctgtcaa aagaaacca caggtcgga gttcgagca actgttaag cttccaaac ctcgagaca tatagctat aagggtaac cgttgtcaa attgaacga gcaaagaca gatagttta ctccaagca gtcagagat cctaaaact gagccttgc caaagggga ggaggtgca gataatggc gcttgacaa agacttgga gtatcctat catcatatc ggctaatat actgtacac ttcccccga ctcacagga aataaatca ggaagaggt cgtgcaaac tgtctccta acgtggtta tttattttg cgtctgcat ttatttgtt atgtacagt atttgttac gtagtgggt tagtaggta tggagagat tttgtgttc ttgcatgag ggtgagttc agtaattcc gtacaaatt tattcacaa atcaaactt taatatcaa aatattgca atatttttg atattattg tttacagtt gatgtgttt tgtgattgc taacaaaaa tatcaaatt cgatttact aatttaata ctgttattt ttttaaacg aggagatta aattaggta ttctaggcg tagtaccta tttaggatt atttaaaag ctttataac agttaccat ttgttaatt ttctaagta aaatattta acaaacttt ctacaccta attttaaga tgtgaaagt gctcttatt aacacctag tcactacgc ttgtcatct aaataaacg gtggattat aacaatttg tcttaatca actgaaccg aacaa
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
