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Gene information
General information
TC032335
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003356
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 2110277..2122703
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032335
    iBeetle screen iB_02691

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032335 RA
      MERTTGQRW HLYVLLASL LTPVTSVEE NQPEIFLSP 
      RNFNPHPYE AGYDPRDAA DANRVGPVV FPPSPPDED 
      NSVNSNVLT LERERQLNH PIDNHPYAK VARKRKYQF 
      PGRFSQQSP PINPKVYEA LSKHGARVL SEPASQLRK 
      DYTLSSLST ERKNYAFSY KVVDHLTGD DFSHTQSQN 
      SRATNGEYR VKLPDGRVQ IVSYTADKN GYKADVKYT 
      ENDLPAPNV QIQQTQIPI PILKPNSYD YHQYLGGFA 
      PYVVSTPKS PLPLYGRQP KVQIYPNGE INGLGNYIG 
      STAAPYLPK AYHGDVVAS TVAPTLAGY TQVYTVY
    CDS sequence
    • >TC032335 RA
      atggagcgg acgaccggg caacgatgg cacctctac 
      gttttactc gcttcactt ctgacaccc gttacaagt 
      gtggaagag aatcaaccc gaaatattt ctttcgccc 
      cgtaatttc aaccctcac ccttacgaa gccggttat 
      gacccgagg gatgctgct gatgcaaat agggtaggc 
      cctgtagtg ttcccacct tcacccccg gacgaagat 
      aactccgtt aatagcaat gttttaact ctagagagg 
      gagcgccaa ctgaatcat ccaatagat aatcacccc 
      tacgcaaaa gtagccagg aaacgaaaa taccaattt 
      ccgggaaga tttagtcaa cagagcccg cccataaat 
      ccgaaggta tatgaagct ttatccaag cacggtgcg 
      agagtgctt tcagagccg gcctctcag ttaaggaag 
      gattacact ctctcctca ttatccacc gagcgcaag 
      aattacgcc ttctcgtat aaagtagtg gatcactta 
      accggcgat gacttctcg cacacgcaa tcccaaaat 
      tcccgcgcg acaaacggc gaatacaga gtcaaattg 
      ccagacggc agggtgcaa atagtctca tacacagcc 
      gacaaaaac ggctacaaa gccgacgtc aaatacacc 
      gaaaacgac ctcccggct cccaatgta caaattcag 
      caaacccaa atcccgata cccattctc aagccgaac 
      tcttacgat taccaccaa tacctaggc ggattcgca 
      ccgtacgtg gtctccaca cccaagagc cccctgccc 
      ttgtacggc agacagccc aaggtgcag atttacccc 
      aacggggaa ataaatgga ttgggtaat tacatcgga 
      tcaactgca gcgccctat ttgcccaag gcgtatcac 
      ggggacgtc gtcgcctcg accgtagct cccacgttg 
      gcgggctac actcaagtg tacactgtg tattaa
    mRNA sequence
    • >TC032335 RA
      ttgatgagc tcgaacgcc aaacttcta attaactat 
      tgtggaagt taattgtgc tctgtttaa tgtaattac 
      ttccatttc cagcatgga gcggacgac cgggcaacg 
      atggcacct ctacgtttt actcgcttc acttctgac 
      acccgttac aagtgtgga agagaatca acccgaaat 
      atttctttc gccccgtaa tttcaaccc tcaccctta 
      cgaagccgg ttatgaccc gagggatgc tgctgatgc 
      aaatagggt aggccctgt agtgttccc accttcacc 
      cccggacga agataactc cgttaatag caatgtttt 
      aactctaga gagggagcg ccaactgaa tcatccaat 
      agataatca cccctacgc aaaagtagc caggaaacg 
      aaaatacca atttccggg aagatttag tcaacagag 
      cccgcccat aaatccgaa ggtatatga agctttatc 
      caagcacgg tgcgagagt gctttcaga gccggcctc 
      tcagttaag gaaggatta cactctctc ctcattatc 
      caccgagcg caagaatta cgccttctc gtataaagt 
      agtggatca cttaaccgg cgatgactt ctcgcacac 
      gcaatccca aaattcccg cgcgacaaa cggcgaata 
      cagagtcaa attgccaga cggcagggt gcaaatagt 
      ctcatacac agccgacaa aaacggcta caaagccga 
      cgtcaaata caccgaaaa cgacctccc ggctcccaa 
      tgtacaaat tcagcaaac ccaaatccc gatacccat 
      tctcaagcc gaactctta cgattacca ccaatacct 
      aggcggatt cgcaccgta cgtggtctc cacacccaa 
      gagccccct gcccttgta cggcagaca gcccaaggt 
      gcagattta ccccaacgg ggaaataaa tggattggg 
      taattacat cggatcaac tgcagcgcc ctatttgcc 
      caaggcgta tcacgggga cgtcgtcgc ctcgaccgt 
      agctcccac gttggcggg ctacactca agtgtacac 
      tgtgtatta acaccgcca acttcagca tcgctcaaa 
      atcaacaag agcacaggc tcggtttat gccaaataa 
      aaattagat tcattaccc ggaaaacac gctcggaat 
      gtttgctgc gttattgta ggggtacat tttaaatta 
      ggtttaacc agaaacaaa tgtacataa gagatggat 
      aatgggctg tgtgtggat acttgccgt tatctattt 
      atttatgta attattcac ttttggttt tgtttagtt 
      cggaatttg tgagcgata ttccgcaca tgctatttt 
      aattaagaa tttttattt tttttattc ctattgtac 
      tagtaaaat accagttta tgcatgttg ttttatgta 
      tttattatt gttgtgtac aggtaaata aacaacttt 
      atcaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY