| General information | |||
| TC003141 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 1211720..1219537 | |
| Sequence information | |||
| Transcripts / Proteins (3) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC003141 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02687 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_09731 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC003141 RA
MEVQAANHV KNREWAQAL EIYNKLLQK QENGEEQLV SYCLDRSEC LYHLGHHER VITDCKHIL KSFSGKYNS TNEPRTRIQ LIRSLFAIN RLAEAEMVI KDWLMMVKQ LNEIGDERI SLENALERF FTLNSKESL EPWLSNLHK SFAKSQSLK KVEKSPAIF CIYCNLSFK DRAELTAHC LTQEHETMI MSDEGHDWY WRAPPRGFK SDAYTLCEN WRELGTCRF GLQCVEAHG EGELYEWKE RFQYREMKM LRAKEKQLY GKSYTENLL EKWVQTPNS NSVMSEKID TVEDACSCP LSATVSSKN SNYSWKFTM KTNKPLKAV ALLQDTHRN HFNLSQIVV GNVEQDIKN NQEFLPNEQ GAVTEYHIV VAFQTTIFG TFRQSVVFD FGSEPVLVK HLCVDVVPD SDLEKINEI RKEITLCMS ERWTKTNSE IVPFFSTLV STGTNHQWE KNLLTTYPY PQIENFVLS NATVSEKKL TRNNYRERM HELLYVEEI ARTNLISQY NLTTRLQIA HSYLLSPNS MAASTAKYS GNGELFASV CLGKELSED TAEGRLILM YCNSVYLLP NPLENRSVR KVYEAIIED KGKDMIYLR LSADTVKEL NLTTDTDLE VQIQFQLNR VPYCEWHYT IDKIRNYKL IFPEIYVEP NIPWSPSRQ WSSSLDSRL NLKQKEAVV AITTPIHVK IPPILVIGP FGTGKTFTL AQAVKELIK DESNKILLC THSNSAADL YIKDYLDVW VESGIENAR PMRIYYQKR WVATVSPVV QKYCSIKTT GNSREFQLP TLEELEKHK IVVVTLSTS VYLSAMGLS PGSFTHILL DEAAQALEC ETITPLALA TENTRIVLA GDHMQLGPD IFSNFAKER NLHISLLER LYDHYPGQF PCKILLCEN YRAHEVIIQ FTSESFYDQ KLVSSSKQP KHFRFYPLT FFTTLGEDV QDKNSTAFY NNSEVYEVV ERVAELKRN WPPEWGEIN EQSLGVVTP YSDQVFRIR SELRKRRID VCVERVLNV QGKQFRAII LSTVRTRRT CTNNKGDSE VDYGFLSNS KLLNTAITR AQSLVAVVG DPIALCSLG RCSKVWERF LQICHDNES LYGITWTQL KFQLDGIEL KKVYTLNPL APEFIPRIY KEMMQENPT FNLPHPKIV PPFPNVPNN IRVVPVVYQ QRPPMTLPY VLNLPTTVA NGYRPLRLV NPHELVPTP RLNVPTPPV PKNAASSTK LIQFMNNVH FPEAQMLND CISLLPQHM SLTDMILQP PSVQERWYN YLRVSAGLE AAEKFKYLL HTINQKGTR IADNNVLGR TTPHSSSDS PTFNWYDSP SNQINFNKP IYMKEVETS NWPDCNKSN PLYKRQSQQ NRAKDSTSQ NGVNKMKES DVQRHVNED FANMSITST SNDDWYEAF KAFNGEQSR TSRPVNNST GFYNYF
- >TC003141 RB
MGLSPGSFT HILLDEAAQ ALECETITP LALATENTR IVLAGDHMQ LGPDIFSNF AKERNLHIS LLERLYDHY PGQFPCKIL LCENYRAHE VIIQFTSES FYDQKLVSS SKQPKHFRF YPLTFFTTL GEDVQDKNS TAFYNNSEV YEVVERVAE LKRNWPPEW GEINEQSLG VVTPYSDQV FRIRSELRK RRIDVCVER VLNVQGKQF RAIILSTVR TRRTCTNNK GDSEVDYGF LSNSKLLNT AITRAQSLV AVVGDPIAL CSLGRCSKV WERFLQICH DNESLYGIT WTQLKFQLD GIELKKVYT LNPLAPEFI PRIYKEMMQ ENPTFNLPH PKIVPPFPN VPNNIRVVP VVYQQRPPM TLPYVLNLP TTVANGYRP LRLVNPHEL VPTPRLNVP TPPVPKNAA SSTKLIQFM NNVHFPEAQ MLNDCISLL PQHMSLTDM ILQPPSVQE RWYNYLRVS AGLEAAEKF KYLLHTINQ KGTRIADNN VLGRTTPHS SSDSPTFNW YDSPSNQIN FNKPIYMKE VETSNWPDC NKSNPLYKR QSQQNRAKD STSQNGVNK MKESDVQRH VNEDFANMS ITSTSNDDW YEAFKAFNG EQSRTSRPV NNSTGFYNY F
- >TC003141 RC
MEVQAANHV KNREWAQAL EIYNKLLQK QENGEEQLV SYCLDRSEC LYHLGHHER VITDCKHIL KSFSGKYNS TNEPRTRIQ LIRSLFAIN RLAEAEMVI KDWLMMVKQ LNEIGDERI SLENALERF FTLNSKESL EPWLSNLHK SFAKSQSLK KVEKSPAIF CIYCNLSFK DRAELTAHC LTQEHETMI MSDEGHDWY WRAPPRGFK SDAYTLCEN WRELGTCRF GLQCVEAHG EGELYEWKE RFQYREMKM LRAKEKQLY GKSYTENLL EKWVQTPNS NSVMSEKID TVEDACSCP LSATVSSKN SNYSWKFTM KTNKPLKAV ALLQDTHRN HFNLSQIVV GNVEQDIKN NQEFLPNEQ GAVTEYHIV VAFQTTIFG TFRQSVVFD FGSEPVLVK HLCVDVVPD SDLEKINEI RKEITLCMS ERWTKTNSE IVPFFSTLV STGTNHQWE KNLLTTYPY PQIENFVLS NATVSEKKL TRNNYRERM HELLYVEEI ARTNLISQY NLTTRLQIA HSYLLSPNS MAASTAKYS GNGELFASV CLGKELSED TAEGRLILM YCNSVYLLP NPLENRSVR KVYEAIIED KGKDMIYLR LSADTVKEL NLTTDTDLE VQIQFQLNR VPYCEWHYT IDKIRNYKL IFPEIYVEP NIPWSPSRQ WSSSLDSRL NLKQKEAVV AITTPIHVK IPPILVIGP FGTGKTFTL AQAVKELIK DESNKILLC THSNSAADL YIKDYLDVW VESGIENAR PMRIYYQKR WVATVSPVV QKVCFINLF HFNLQDFNA EIHL
- >TC003141 RA
atggaagtc caggctgca aatcacgtc aaaaacaga gaatgggcc caggcttta gaaatttac aataaattg ttacaaaag caggaaaac ggtgaagag cagctcgtt tcgtattgt ttggaccgc tctgagtgt ctctaccat ttggggcac cacgaacgg gtcataacc gactgcaaa cacattttg aagtctttc agtggcaaa tacaacagt acaaacgaa ccccgaaca agaatccag ctaattcgc tccttattc gcgataaat cgtctcgca gaagcagaa atggtgatc aaagactgg ttaatgatg gttaaacaa ctgaacgaa attggagac gaaagaatc tcgttggaa aacgcgctg gaacgtttc tttactctc aactcaaag gaaagcctc gagccctgg ctgagtaac ttgcacaaa agttttgcc aaaagccag tcgctgaag aaagtggaa aaaagtccg gcaattttt tgcatttat tgcaacctt tcgtttaaa gatcgggcg gaactgacg gctcattgc ctgactcag gagcatgaa accatgatt atgtcagat gaggggcac gactggtac tggagggcc ccgccaagg ggcttcaag tcggatgct tacaccctt tgcgagaat tggagggag ttggggact tgtcggttc gggcttcag tgtgtggaa gcccacgga gaaggcgaa ctctacgag tggaaggaa cggtttcag tatcgggag atgaaaatg ttgagggcg aaagaaaaa caactctat ggcaagtct tacactgag aatctcctc gaaaagtgg gtgcaaacc ccgaacagt aactcggtc atgtccgaa aaaatcgac actgttgaa gacgcctgt tcttgtccg ctttcagcg acagtttcc tccaaaaac agcaactat tcgtggaaa tttacaatg aaaactaac aaacctctg aaagctgtg gccctcttg caagacacg caccgcaac cacttcaac ttatcccaa atagttgtc ggaaacgtg gaacaagat attaaaaat aaccaggaa tttttacca aacgaacag ggggctgtg accgagtac cacattgtt gtcgctttc caaacaaca attttcggc acttttcgc cagtctgtt gttttcgat ttcgggtcc gaaccggtt ttggtgaaa cacctctgt gttgatgtt gttcccgac tcagatctc gagaaaatt aacgagatt cgtaaagag ataacgctt tgcatgtct gagaggtgg actaaaacc aactctgaa atcgtcccg tttttctcg actttagtt tcaactggg actaaccac caatgggag aaaaacctt ctaacgact tatccgtac ccacaaatt gagaacttt gtattatct aatgccaca gtttccgag aaaaaatta actaggaat aattatcgg gaacgaatg cacgaattg ctctacgtt gaagaaata gcacggacg aatttaatt tctcagtat aatttaaca acacggctt caaatagcg catagttat ctcttgtcg ccgaatagc atggccgct agtacggct aaatattcg ggaaacggt gaattattc gcgtctgtt tgtttaggt aaagagttg tcggaggat acggctgag ggtcgcttg atcctcatg tactgcaac tcggtttat ttgcttcca aaccctttg gaaaatcga tcggtgcgc aaggtctac gaagccata attgaagat aaaggcaaa gacatgatt tatttaaga ctctctgcc gatactgtt aaagaacta aatctgacg actgataca gacttggaa gtgcaaatc caattccaa ctgaaccgt gttccctac tgcgagtgg cactacacg atcgataaa atccgaaat tataagtta atttttcct gagatttat gttgagcca aatattccg tggtcgccg tcaaggcag tggtcttcg tctctagat agccgtttg aatttgaag caaaaggag gctgtggtt gcgattaca acgccgatt catgttaaa atccccccg attttggtg attgggcct ttcgggact gggaaaacg ttcactttg gctcaagcg gttaaagag ttgattaaa gatgagagt aataagatt ttgttgtgt acgcattcg aacagcgcc gctgatttg tacatcaag gattatttg gacgtttgg gtcgaaagt gggatcgaa aatgcgagg ccgatgagg atttattac cagaagaga tgggtggcg actgtcagt ccggtggtt caaaagtac tgcagtatt aaaacgacc ggtaattcc cgcgaattc caacttcca accctcgaa gagttggaa aaacacaaa atcgttgta gtaacactt tcaacatcc gtctactta tcagcgatg ggattatca cccggcagc ttcacgcac attttatta gacgaagcg gctcaagcg ctcgaatgc gaaacgata acccctctg gcattagca acagaaaat acgagaata gtcctagcc ggcgatcac atgcaatta ggacctgac atcttctcg aatttcgca aaagaacgc aatttgcat atttcgctc ctcgagcga ttatacgac cactatccg ggccaattt ccttgcaaa atactccta tgcgaaaac tacagggcc cacgaagtc ataatccag ttcacttcc gaatcgttc tacgaccaa aaactggtc tcgagcagc aaacaaccg aaacatttc cggttttat cctcttacg tttttcacg acattgggc gaggacgtg caagataaa aactcgacg gcgttttac aataattcc gaagtttat gaagtagtg gagagagtg gcagagctc aagcgaaat tggccccca gaatggggc gaaatcaac gagcaatcg ctcggtgtc gtcactccg tattcggat caagtgttc aggatacgt tcagagtta cggaaacgt agaatcgat gtttgtgtg gaacgagtt ttgaacgtt caagggaag cagtttagg gccattatc ctatcaacg gtgcggacg agacgcact tgcaccaac aataaaggg gacagtgaa gtggattat ggattttta tcaaactcc aaactgcta aacactgcg attacgaga gcgcagagt ttggtggca gttgtgggc gacccgatt gccttatgc tccctaggg aggtgtagc aaagtctgg gagcgtttc ttgcagatt tgtcacgac aacgaaagt ctttacggc atcacgtgg acacaactt aaatttcaa ttggacgga atcgaattg aaaaaagtc tacactttg aatccgcta gctcccgag tttattccc cggatatac aaagaaatg atgcaagaa aacccaact tttaactta cctcacccc aaaatagtg ccccctttt cccaacgtc ccgaacaac ataagggtg gtgccggtg gtgtaccaa cagagaccc ccaatgact cttccttac gtccttaat ttgccaacg actgtggca aatgggtac cgaccatta cgactcgtt aacccccat gaactcgtg cccacaccg agactcaac gtaccaacg cctccggta ccaaaaaat gcagcctcc agcacaaaa ctgatccaa tttatgaat aatgtccac ttccccgaa gcccaaatg ttgaatgat tgtatcagt ttgctgccg cagcacatg tccctcact gatatgatt ctgcagccc cctagtgtg caagagcgg tggtacaac tacttgagg gtctctgcg ggtctagag gctgcagaa aaattcaag tatttgctt cataccatc aatcagaag gggacaagg atagctgat aataatgta ttagggaga acaaccccg cactcatcg tcagattcg cccacgttt aactggtac gacagccct tccaatcag attaatttt aataaaccg atttatatg aaagaagtt gagactagt aattggcca gattgtaac aagtcgaat ccgttgtat aaacgacag agtcaacaa aaccgtgct aaagactcg acaagtcaa aatggggtt aacaaaatg aaagaaagc gacgttcag aggcacgtt aacgaggat tttgccaat atgagtatt actagcacc agtaacgat gactggtat gaggcgttt aaggcattt aatggtgaa caaagccgt actagcaga cctgtgaat aattcaacc gggttttac aattatttc tag
- >TC003141 RB
atgggatta tcacccggc agcttcacg cacatttta ttagacgaa gcggctcaa gcgctcgaa tgcgaaacg ataacccct ctggcatta gcaacagaa aatacgaga atagtccta gccggcgat cacatgcaa ttaggacct gacatcttc tcgaatttc gcaaaagaa cgcaatttg catatttcg ctcctcgag cgattatac gaccactat ccgggccaa tttccttgc aaaatactc ctatgcgaa aactacagg gcccacgaa gtcataatc cagttcact tccgaatcg ttctacgac caaaaactg gtctcgagc agcaaacaa ccgaaacat ttccggttt tatcctctt acgtttttc acgacattg ggcgaggac gtgcaagat aaaaactcg acggcgttt tacaataat tccgaagtt tatgaagta gtggagaga gtggcagag ctcaagcga aattggccc ccagaatgg ggcgaaatc aacgagcaa tcgctcggt gtcgtcact ccgtattcg gatcaagtg ttcaggata cgttcagag ttacggaaa cgtagaatc gatgtttgt gtggaacga gttttgaac gttcaaggg aagcagttt agggccatt atcctatca acggtgcgg acgagacgc acttgcacc aacaataaa ggggacagt gaagtggat tatggattt ttatcaaac tccaaactg ctaaacact gcgattacg agagcgcag agtttggtg gcagttgtg ggcgacccg attgcctta tgctcccta gggaggtgt agcaaagtc tgggagcgt ttcttgcag atttgtcac gacaacgaa agtctttac ggcatcacg tggacacaa cttaaattt caattggac ggaatcgaa ttgaaaaaa gtctacact ttgaatccg ctagctccc gagtttatt ccccggata tacaaagaa atgatgcaa gaaaaccca acttttaac ttacctcac cccaaaata gtgccccct tttcccaac gtcccgaac aacataagg gtggtgccg gtggtgtac caacagaga cccccaatg actcttcct tacgtcctt aatttgcca acgactgtg gcaaatggg taccgacca ttacgactc gttaacccc catgaactc gtgcccaca ccgagactc aacgtacca acgcctccg gtaccaaaa aatgcagcc tccagcaca aaactgatc caatttatg aataatgtc cacttcccc gaagcccaa atgttgaat gattgtatc agtttgctg ccgcagcac atgtccctc actgatatg attctgcag ccccctagt gtgcaagag cggtggtac aactacttg agggtctct gcgggtcta gaggctgca gaaaaattc aagtatttg cttcatacc atcaatcag aaggggaca aggatagct gataataat gtattaggg agaacaacc ccgcactca tcgtcagat tcgcccacg tttaactgg tacgacagc ccttccaat cagattaat tttaataaa ccgatttat atgaaagaa gttgagact agtaattgg ccagattgt aacaagtcg aatccgttg tataaacga cagagtcaa caaaaccgt gctaaagac tcgacaagt caaaatggg gttaacaaa atgaaagaa agcgacgtt cagaggcac gttaacgag gattttgcc aatatgagt attactagc accagtaac gatgactgg tatgaggcg tttaaggca tttaatggt gaacaaagc cgtactagc agacctgtg aataattca accgggttt tacaattat ttctag
- >TC003141 RC
atggaagtc caggctgca aatcacgtc aaaaacaga gaatgggcc caggcttta gaaatttac aataaattg ttacaaaag caggaaaac ggtgaagag cagctcgtt tcgtattgt ttggaccgc tctgagtgt ctctaccat ttggggcac cacgaacgg gtcataacc gactgcaaa cacattttg aagtctttc agtggcaaa tacaacagt acaaacgaa ccccgaaca agaatccag ctaattcgc tccttattc gcgataaat cgtctcgca gaagcagaa atggtgatc aaagactgg ttaatgatg gttaaacaa ctgaacgaa attggagac gaaagaatc tcgttggaa aacgcgctg gaacgtttc tttactctc aactcaaag gaaagcctc gagccctgg ctgagtaac ttgcacaaa agttttgcc aaaagccag tcgctgaag aaagtggaa aaaagtccg gcaattttt tgcatttat tgcaacctt tcgtttaaa gatcgggcg gaactgacg gctcattgc ctgactcag gagcatgaa accatgatt atgtcagat gaggggcac gactggtac tggagggcc ccgccaagg ggcttcaag tcggatgct tacaccctt tgcgagaat tggagggag ttggggact tgtcggttc gggcttcag tgtgtggaa gcccacgga gaaggcgaa ctctacgag tggaaggaa cggtttcag tatcgggag atgaaaatg ttgagggcg aaagaaaaa caactctat ggcaagtct tacactgag aatctcctc gaaaagtgg gtgcaaacc ccgaacagt aactcggtc atgtccgaa aaaatcgac actgttgaa gacgcctgt tcttgtccg ctttcagcg acagtttcc tccaaaaac agcaactat tcgtggaaa tttacaatg aaaactaac aaacctctg aaagctgtg gccctcttg caagacacg caccgcaac cacttcaac ttatcccaa atagttgtc ggaaacgtg gaacaagat attaaaaat aaccaggaa tttttacca aacgaacag ggggctgtg accgagtac cacattgtt gtcgctttc caaacaaca attttcggc acttttcgc cagtctgtt gttttcgat ttcgggtcc gaaccggtt ttggtgaaa cacctctgt gttgatgtt gttcccgac tcagatctc gagaaaatt aacgagatt cgtaaagag ataacgctt tgcatgtct gagaggtgg actaaaacc aactctgaa atcgtcccg tttttctcg actttagtt tcaactggg actaaccac caatgggag aaaaacctt ctaacgact tatccgtac ccacaaatt gagaacttt gtattatct aatgccaca gtttccgag aaaaaatta actaggaat aattatcgg gaacgaatg cacgaattg ctctacgtt gaagaaata gcacggacg aatttaatt tctcagtat aatttaaca acacggctt caaatagcg catagttat ctcttgtcg ccgaatagc atggccgct agtacggct aaatattcg ggaaacggt gaattattc gcgtctgtt tgtttaggt aaagagttg tcggaggat acggctgag ggtcgcttg atcctcatg tactgcaac tcggtttat ttgcttcca aaccctttg gaaaatcga tcggtgcgc aaggtctac gaagccata attgaagat aaaggcaaa gacatgatt tatttaaga ctctctgcc gatactgtt aaagaacta aatctgacg actgataca gacttggaa gtgcaaatc caattccaa ctgaaccgt gttccctac tgcgagtgg cactacacg atcgataaa atccgaaat tataagtta atttttcct gagatttat gttgagcca aatattccg tggtcgccg tcaaggcag tggtcttcg tctctagat agccgtttg aatttgaag caaaaggag gctgtggtt gcgattaca acgccgatt catgttaaa atccccccg attttggtg attgggcct ttcgggact gggaaaacg ttcactttg gctcaagcg gttaaagag ttgattaaa gatgagagt aataagatt ttgttgtgt acgcattcg aacagcgcc gctgatttg tacatcaag gattatttg gacgtttgg gtcgaaagt gggatcgaa aatgcgagg ccgatgagg atttattac cagaagaga tgggtggcg actgtcagt ccggtggtt caaaaggtt tgttttatt aacttattt catttcaat cttcaagat ttcaatgcg gaaatccac ctctag
- >TC003141 RA
aaacactcg ttttaattg gaacggttg cgtttttta tggttgaca accaatgac tttagaatc tgacagtag tgtcaaaat ttgccgtaa cccgaccca caagtttca gtcaacaat gaaagaaaa ataccgata gaatttgat ttcgattaa aaccttact ttttccaaa caatgtgaa cccgtttta aggtgaaca atcgagaat tagatgccc tttgaaccc cctttacgc catggaagt ccaggctgc aaatcacgt caaaaacag agaatgggc ccaggcttt agaaattta caataaatt gttacaaaa gcaggaaaa cggtgaaga gcagctcgt ttcgtattg tttggaccg ctctgagtg tctctacca tttggggca ccacgaacg ggtcataac cgactgcaa acacatttt gaagtcttt cagtggcaa atacaacag tacaaacga accccgaac aagaatcca gctaattcg ctccttatt cgcgataaa tcgtctcgc agaagcaga aatggtgat caaagactg gttaatgat ggttaaaca actgaacga aattggaga cgaaagaat ctcgttgga aaacgcgct ggaacgttt ctttactct caactcaaa ggaaagcct cgagccctg gctgagtaa cttgcacaa aagttttgc caaaagcca gtcgctgaa gaaagtgga aaaaagtcc ggcaatttt ttgcattta ttgcaacct ttcgtttaa agatcgggc ggaactgac ggctcattg cctgactca ggagcatga aaccatgat tatgtcaga tgaggggca cgactggta ctggagggc cccgccaag gggcttcaa gtcggatgc ttacaccct ttgcgagaa ttggaggga gttggggac ttgtcggtt cgggcttca gtgtgtgga agcccacgg agaaggcga actctacga gtggaagga acggtttca gtatcggga gatgaaaat gttgagggc gaaagaaaa acaactcta tggcaagtc ttacactga gaatctcct cgaaaagtg ggtgcaaac cccgaacag taactcggt catgtccga aaaaatcga cactgttga agacgcctg ttcttgtcc gctttcagc gacagtttc ctccaaaaa cagcaacta ttcgtggaa atttacaat gaaaactaa caaacctct gaaagctgt ggccctctt gcaagacac gcaccgcaa ccacttcaa cttatccca aatagttgt cggaaacgt ggaacaaga tattaaaaa taaccagga atttttacc aaacgaaca gggggctgt gaccgagta ccacattgt tgtcgcttt ccaaacaac aattttcgg cacttttcg ccagtctgt tgttttcga tttcgggtc cgaaccggt tttggtgaa acacctctg tgttgatgt tgttcccga ctcagatct cgagaaaat taacgagat tcgtaaaga gataacgct ttgcatgtc tgagaggtg gactaaaac caactctga aatcgtccc gtttttctc gactttagt ttcaactgg gactaacca ccaatggga gaaaaacct tctaacgac ttatccgta cccacaaat tgagaactt tgtattatc taatgccac agtttccga gaaaaaatt aactaggaa taattatcg ggaacgaat gcacgaatt gctctacgt tgaagaaat agcacggac gaatttaat ttctcagta taatttaac aacacggct tcaaatagc gcatagtta tctcttgtc gccgaatag catggccgc tagtacggc taaatattc gggaaacgg tgaattatt cgcgtctgt ttgtttagg taaagagtt gtcggagga tacggctga gggtcgctt gatcctcat gtactgcaa ctcggttta tttgcttcc aaacccttt ggaaaatcg atcggtgcg caaggtcta cgaagccat aattgaaga taaaggcaa agacatgat ttatttaag actctctgc cgatactgt taaagaact aaatctgac gactgatac agacttgga agtgcaaat ccaattcca actgaaccg tgttcccta ctgcgagtg gcactacac gatcgataa aatccgaaa ttataagtt aatttttcc tgagattta tgttgagcc aaatattcc gtggtcgcc gtcaaggca gtggtcttc gtctctaga tagccgttt gaatttgaa gcaaaagga ggctgtggt tgcgattac aacgccgat tcatgttaa aatcccccc gattttggt gattgggcc tttcgggac tgggaaaac gttcacttt ggctcaagc ggttaaaga gttgattaa agatgagag taataagat tttgttgtg tacgcattc gaacagcgc cgctgattt gtacatcaa ggattattt ggacgtttg ggtcgaaag tgggatcga aaatgcgag gccgatgag gatttatta ccagaagag atgggtggc gactgtcag tccggtggt tcaaaagta ctgcagtat taaaacgac cggtaattc ccgcgaatt ccaacttcc aaccctcga agagttgga aaaacacaa aatcgttgt agtaacact ttcaacatc cgtctactt atcagcgat gggattatc acccggcag cttcacgca cattttatt agacgaagc ggctcaagc gctcgaatg cgaaacgat aacccctct ggcattagc aacagaaaa tacgagaat agtcctagc cggcgatca catgcaatt aggacctga catcttctc gaatttcgc aaaagaacg caatttgca tatttcgct cctcgagcg attatacga ccactatcc gggccaatt tccttgcaa aatactcct atgcgaaaa ctacagggc ccacgaagt cataatcca gttcacttc cgaatcgtt ctacgacca aaaactggt ctcgagcag caaacaacc gaaacattt ccggtttta tcctcttac gtttttcac gacattggg cgaggacgt gcaagataa aaactcgac ggcgtttta caataattc cgaagttta tgaagtagt ggagagagt ggcagagct caagcgaaa ttggccccc agaatgggg cgaaatcaa cgagcaatc gctcggtgt cgtcactcc gtattcgga tcaagtgtt caggatacg ttcagagtt acggaaacg tagaatcga tgtttgtgt ggaacgagt tttgaacgt tcaagggaa gcagtttag ggccattat cctatcaac ggtgcggac gagacgcac ttgcaccaa caataaagg ggacagtga agtggatta tggattttt atcaaactc caaactgct aaacactgc gattacgag agcgcagag tttggtggc agttgtggg cgacccgat tgccttatg ctccctagg gaggtgtag caaagtctg ggagcgttt cttgcagat ttgtcacga caacgaaag tctttacgg catcacgtg gacacaact taaatttca attggacgg aatcgaatt gaaaaaagt ctacacttt gaatccgct agctcccga gtttattcc ccggatata caaagaaat gatgcaaga aaacccaac ttttaactt acctcaccc caaaatagt gcccccttt tcccaacgt cccgaacaa cataagggt ggtgccggt ggtgtacca acagagacc cccaatgac tcttcctta cgtccttaa tttgccaac gactgtggc aaatgggta ccgaccatt acgactcgt taaccccca tgaactcgt gcccacacc gagactcaa cgtaccaac gcctccggt accaaaaaa tgcagcctc cagcacaaa actgatcca atttatgaa taatgtcca cttccccga agcccaaat gttgaatga ttgtatcag tttgctgcc gcagcacat gtccctcac tgatatgat tctgcagcc ccctagtgt gcaagagcg gtggtacaa ctacttgag ggtctctgc gggtctaga ggctgcaga aaaattcaa gtatttgct tcataccat caatcagaa ggggacaag gatagctga taataatgt attagggag aacaacccc gcactcatc gtcagattc gcccacgtt taactggta cgacagccc ttccaatca gattaattt taataaacc gatttatat gaaagaagt tgagactag taattggcc agattgtaa caagtcgaa tccgttgta taaacgaca gagtcaaca aaaccgtgc taaagactc gacaagtca aaatggggt taacaaaat gaaagaaag cgacgttca gaggcacgt taacgagga ttttgccaa tatgagtat tactagcac cagtaacga tgactggta tgaggcgtt taaggcatt taatggtga acaaagccg tactagcag acctgtgaa taattcaac cgggtttta caattattt ctagtaaaa tcttttgta tattgtata tattatgca tatagctta tcgctgttg ttacgtgtt tgattgtag atacagtta agagagctt ttttttacg at
- >TC003141 RB
agtactgca gtattaaaa cgaccggta attcccgcg aattccaac ttccaaccc tcgaagagt tggaaaaac acaaaatcg ttgtagtaa cactttcaa catccgtct acttatcag cgatgggat tatcacccg gcagcttca cgcacattt tattagacg aagcggctc aagcgctcg aatgcgaaa cgataaccc ctctggcat tagcaacag aaaatacga gaatagtcc tagccggcg atcacatgc aattaggac ctgacatct tctcgaatt tcgcaaaag aacgcaatt tgcatattt cgctcctcg agcgattat acgaccact atccgggcc aatttcctt gcaaaatac tcctatgcg aaaactaca gggcccacg aagtcataa tccagttca cttccgaat cgttctacg accaaaaac tggtctcga gcagcaaac aaccgaaac atttccggt tttatcctc ttacgtttt tcacgacat tgggcgagg acgtgcaag ataaaaact cgacggcgt tttacaata attccgaag tttatgaag tagtggaga gagtggcag agctcaagc gaaattggc ccccagaat ggggcgaaa tcaacgagc aatcgctcg gtgtcgtca ctccgtatt cggatcaag tgttcagga tacgttcag agttacgga aacgtagaa tcgatgttt gtgtggaac gagttttga acgttcaag ggaagcagt ttagggcca ttatcctat caacggtgc ggacgagac gcacttgca ccaacaata aaggggaca gtgaagtgg attatggat ttttatcaa actccaaac tgctaaaca ctgcgatta cgagagcgc agagtttgg tggcagttg tgggcgacc cgattgcct tatgctccc tagggaggt gtagcaaag tctgggagc gtttcttgc agatttgtc acgacaacg aaagtcttt acggcatca cgtggacac aacttaaat ttcaattgg acggaatcg aattgaaaa aagtctaca ctttgaatc cgctagctc ccgagttta ttccccgga tatacaaag aaatgatgc aagaaaacc caactttta acttacctc accccaaaa tagtgcccc cttttccca acgtcccga acaacataa gggtggtgc cggtggtgt accaacaga gacccccaa tgactcttc cttacgtcc ttaatttgc caacgactg tggcaaatg ggtaccgac cattacgac tcgttaacc cccatgaac tcgtgccca caccgagac tcaacgtac caacgcctc cggtaccaa aaaatgcag cctccagca caaaactga tccaattta tgaataatg tccacttcc ccgaagccc aaatgttga atgattgta tcagtttgc tgccgcagc acatgtccc tcactgata tgattctgc agcccccta gtgtgcaag agcggtggt acaactact tgagggtct ctgcgggtc tagaggctg cagaaaaat tcaagtatt tgcttcata ccatcaatc agaagggga caaggatag ctgataata atgtattag ggagaacaa ccccgcact catcgtcag attcgccca cgtttaact ggtacgaca gcccttcca atcagatta attttaata aaccgattt atatgaaag aagttgaga ctagtaatt ggccagatt gtaacaagt cgaatccgt tgtataaac gacagagtc aacaaaacc gtgctaaag actcgacaa gtcaaaatg gggttaaca aaatgaaag aaagcgacg ttcagaggc acgttaacg aggattttg ccaatatga gtattacta gcaccagta acgatgact ggtatgagg cgtttaagg catttaatg gtgaacaaa gccgtacta gcagacctg tgaataatt caaccgggt tttacaatt atttctagt aaaatcttt tgtatattg tatatatta tgcatatag cttatcgct gttgttacg tgtttgatt gtagataca gttaagaga gcttttttt tacgat
- >TC003141 RC
aaacactcg ttttaattg gaacggttg cgtttttta tggttgaca accaatgac tttagaatc tgacagtag tgtcaaaat ttgccgtaa cccgaccca caagtttca gtcaacaat gaaagaaaa ataccgata gaatttgat ttcgattaa aaccttact ttttccaaa caatgtgaa cccgtttta aggtgaaca atcgagaat tagatgccc tttgaaccc cctttacgc catggaagt ccaggctgc aaatcacgt caaaaacag agaatgggc ccaggcttt agaaattta caataaatt gttacaaaa gcaggaaaa cggtgaaga gcagctcgt ttcgtattg tttggaccg ctctgagtg tctctacca tttggggca ccacgaacg ggtcataac cgactgcaa acacatttt gaagtcttt cagtggcaa atacaacag tacaaacga accccgaac aagaatcca gctaattcg ctccttatt cgcgataaa tcgtctcgc agaagcaga aatggtgat caaagactg gttaatgat ggttaaaca actgaacga aattggaga cgaaagaat ctcgttgga aaacgcgct ggaacgttt ctttactct caactcaaa ggaaagcct cgagccctg gctgagtaa cttgcacaa aagttttgc caaaagcca gtcgctgaa gaaagtgga aaaaagtcc ggcaatttt ttgcattta ttgcaacct ttcgtttaa agatcgggc ggaactgac ggctcattg cctgactca ggagcatga aaccatgat tatgtcaga tgaggggca cgactggta ctggagggc cccgccaag gggcttcaa gtcggatgc ttacaccct ttgcgagaa ttggaggga gttggggac ttgtcggtt cgggcttca gtgtgtgga agcccacgg agaaggcga actctacga gtggaagga acggtttca gtatcggga gatgaaaat gttgagggc gaaagaaaa acaactcta tggcaagtc ttacactga gaatctcct cgaaaagtg ggtgcaaac cccgaacag taactcggt catgtccga aaaaatcga cactgttga agacgcctg ttcttgtcc gctttcagc gacagtttc ctccaaaaa cagcaacta ttcgtggaa atttacaat gaaaactaa caaacctct gaaagctgt ggccctctt gcaagacac gcaccgcaa ccacttcaa cttatccca aatagttgt cggaaacgt ggaacaaga tattaaaaa taaccagga atttttacc aaacgaaca gggggctgt gaccgagta ccacattgt tgtcgcttt ccaaacaac aattttcgg cacttttcg ccagtctgt tgttttcga tttcgggtc cgaaccggt tttggtgaa acacctctg tgttgatgt tgttcccga ctcagatct cgagaaaat taacgagat tcgtaaaga gataacgct ttgcatgtc tgagaggtg gactaaaac caactctga aatcgtccc gtttttctc gactttagt ttcaactgg gactaacca ccaatggga gaaaaacct tctaacgac ttatccgta cccacaaat tgagaactt tgtattatc taatgccac agtttccga gaaaaaatt aactaggaa taattatcg ggaacgaat gcacgaatt gctctacgt tgaagaaat agcacggac gaatttaat ttctcagta taatttaac aacacggct tcaaatagc gcatagtta tctcttgtc gccgaatag catggccgc tagtacggc taaatattc gggaaacgg tgaattatt cgcgtctgt ttgtttagg taaagagtt gtcggagga tacggctga gggtcgctt gatcctcat gtactgcaa ctcggttta tttgcttcc aaacccttt ggaaaatcg atcggtgcg caaggtcta cgaagccat aattgaaga taaaggcaa agacatgat ttatttaag actctctgc cgatactgt taaagaact aaatctgac gactgatac agacttgga agtgcaaat ccaattcca actgaaccg tgttcccta ctgcgagtg gcactacac gatcgataa aatccgaaa ttataagtt aatttttcc tgagattta tgttgagcc aaatattcc gtggtcgcc gtcaaggca gtggtcttc gtctctaga tagccgttt gaatttgaa gcaaaagga ggctgtggt tgcgattac aacgccgat tcatgttaa aatcccccc gattttggt gattgggcc tttcgggac tgggaaaac gttcacttt ggctcaagc ggttaaaga gttgattaa agatgagag taataagat tttgttgtg tacgcattc gaacagcgc cgctgattt gtacatcaa ggattattt ggacgtttg ggtcgaaag tgggatcga aaatgcgag gccgatgag gatttatta ccagaagag atgggtggc gactgtcag tccggtggt tcaaaaggt ttgttttat taacttatt tcatttcaa tcttcaaga tttcaatgc ggaaatcca cctctagat aaacaaaaa tgaaaaatc atgacttta aacctgaat tatct
