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Gene information
General information
TC003283 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid13: 724260..726374
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003283
    iBeetle screen iB_02685
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003283 RA
      MATSSNSQM EASLNLCTN STAQELLKC FFCDKFLSV 
      PPIYNHEFE SICGRCKFV NKSEDSKWT RQTLYEDVA 
      RFMIFPCSN STYGCKAQL KWGDVEEHE NTCLYKKLN 
      CPFACNDIF HVEKCRWMG SGPGLNEHL EFCHGDVIK 
      KLPEINFSE SRANGIFLT RILKQLMIV VVKYDPPDT 
      FSCMVMVNG TSTDCEAYR YQLELFDEN KRNSLILRR 
      SRLEDAVGF EENLTNPEK MLVVELQVV KSLLKKPAT 
      IMGRFGIIK KSKRELNFG DKPQECPFD ENFLRELEC 
      PVCKNYMVP PIQICSTGH SFCSRCRDQ MEECPTCRH 
      PFQEGRNYT LEKLTTCIN YPCMFRDAG CTVACPSEK 
      LREHELDCS FSGIQCFLE CNTGPVMNL FKHLNEKHR 
      DRLIVAGEV HILDLGEGE VMKTFAICF GNDMFRLAV 
      FYDGDIKFS LQQFGVKQS CYTYELEII CTTDCSEKI 
      ILTKECPPM STTFWSKSI TVPDDLIQR FISEEDLVF 
      FKINLRKMV
    CDS sequence
    • >TC003283 RA
      atggcaacg agttcaaat agccaaatg gaggcctcc 
      ctcaacttg tgcaccaac tccacggct caggagctc 
      ctgaagtgt tttttttgc gacaaattc ctctcagta 
      ccccccatt tacaaccac gagtttgag tcgatttgt 
      ggacgttgc aaattcgtc aacaaatcg gaggactcc 
      aagtggacg cgccaaacc ctctacgaa gacgtcgcc 
      aggtttatg atttttcct tgctccaat tccacatac 
      ggctgtaaa gcccagctg aaatggggc gacgtggag 
      gagcacgaa aacacctgt ctctacaag aagttgaac 
      tgtcccttc gcctgtaac gacatcttc cacgtggag 
      aagtgccgc tggatgggc tccggcccc ggtctgaac 
      gaacacctg gagttctgc cacggcgac gtcattaaa 
      aaactcccc gaaatcaat ttcagcgaa agccgcgcc 
      aatggaatt ttcctaact aggatcctc aaacaacta 
      atgatcgtg gtggtgaaa tacgaccct ccggacacc 
      ttctcctgc atggtgatg gtcaacggc accagcacc 
      gactgcgag gcctaccgt taccaactg gagctcttc 
      gatgaaaac aaacgcaac tctttgatt ttgcgccga 
      agtcgcttg gaagacgcc gtcggtttc gaggagaat 
      ctaaccaac ccggagaaa atgctcgtg gtggagctc 
      caggtggtg aaatccctc ctgaagaag ccggcaacc 
      atcatgggc cggttcggt ataattaaa aaatcgaaa 
      cgcgagctc aacttcggg gacaagccc caggagtgc 
      cccttcgat gagaacttc ctaagagag ttggagtgt 
      cccgtgtgt aagaactac atggtgccc ccgatccag 
      atttgcagc acggggcat agtttctgc tccaggtgt 
      cgcgaccag atggaggag tgccccacg tgtcggcac 
      cccttccaa gaggggcgg aactacacg ttggagaag 
      ctcaccacg tgtattaat tatccttgt atgtttcgg 
      gatgccggg tgcacggtt gcgtgtcct agtgaaaag 
      ttgagggag catgagttg gattgtagt ttttcgggg 
      attcagtgc ttcctggag tgcaatacg ggtccagtg 
      atgaacttg ttcaagcat ttgaatgag aaacaccga 
      gatcggttg attgttgcc ggagaggtg catattttg 
      gacctgggg gagggggag gtcatgaag acgtttgcc 
      atttgcttc gggaatgat atgttccga ttggcggtg 
      ttttacgat ggggatatt aaattctcg ttgcaacag 
      tttggggtt aaacaaagt tgttacact tatgagttg 
      gagattatt tgtacgacg gattgttcc gagaagatt 
      atcttgacg aaggagtgt ccacccatg agtacgacc 
      ttctggtcc aagagcatt acggtcccg gatgatttg 
      atacaaagg ttcatctcg gaggaggat ttggtcttt 
      tttaaaatc aatttgagg aaaatggtt tag
    mRNA sequence
    • >TC003283 RA
      gttgtgtta ttattatta cttacttta caatacatt 
      taaaaattg tttttttaa ttgaatttt ttaaagttg 
      gtaccggca cttatcgcc ttatcaacg gttgagtgt 
      gtggtttac cgataaggg ttttgtatt tatttgtgc 
      ataaaacag tctaaacga gtccgatta acaaataat 
      acccaatct atttaataa taattaatt tggcgtgat 
      tgacgttat gtcaggtta gcaggattg ccaatcaga 
      taaggggga ccccctttc acgacaccc aatttaggt 
      taataccac ttaattcaa acaatcaaa cttcctgtt 
      tcaataata aaaactaat tgattgtta actgattag 
      ggaaattgg tattgtcac gttggcgac acttaatga 
      atttttcaa ataaataac ccgcaaaca atattgtct 
      ctattcgtt taaataatg gcaacgagt tcaaatagc 
      caaatggag gcctccctc aacttgtgc accaactcc 
      acggctcag gagctcctg aagtgtttt ttttgcgac 
      aaattcctc tcagtaccc cccatttac aaccacgag 
      tttgagtcg atttgtgga cgttgcaaa ttcgtcaac 
      aaatcggag gactccaag tggacgcgc caaaccctc 
      tacgaagac gtcgccagg tttatgatt tttccttgc 
      tccaattcc acatacggc tgtaaagcc cagctgaaa 
      tggggcgac gtggaggag cacgaaaac acctgtctc 
      tacaagaag ttgaactgt cccttcgcc tgtaacgac 
      atcttccac gtggagaag tgccgctgg atgggctcc 
      ggccccggt ctgaacgaa cacctggag ttctgccac 
      ggcgacgtc attaaaaaa ctccccgaa atcaatttc 
      agcgaaagc cgcgccaat ggaattttc ctaactagg 
      atcctcaaa caactaatg atcgtggtg gtgaaatac 
      gaccctccg gacaccttc tcctgcatg gtgatggtc 
      aacggcacc agcaccgac tgcgaggcc taccgttac 
      caactggag ctcttcgat gaaaacaaa cgcaactct 
      ttgattttg cgccgaagt cgcttggaa gacgccgtc 
      ggtttcgag gagaatcta accaacccg gagaaaatg 
      ctcgtggtg gagctccag gtggtgaaa tccctcctg 
      aagaagccg gcaaccatc atgggccgg ttcggtata 
      attaaaaaa tcgaaacgc gagctcaac ttcggggac 
      aagccccag gagtgcccc ttcgatgag aacttccta 
      agagagttg gagtgtccc gtgtgtaag aactacatg 
      gtgcccccg atccagatt tgcagcacg gggcatagt 
      ttctgctcc aggtgtcgc gaccagatg gaggagtgc 
      cccacgtgt cggcacccc ttccaagag gggcggaac 
      tacacgttg gagaagctc accacgtgt attaattat 
      ccttgtatg tttcgggat gccgggtgc acggttgcg 
      tgtcctagt gaaaagttg agggagcat gagttggat 
      tgtagtttt tcggggatt cagtgcttc ctggagtgc 
      aatacgggt ccagtgatg aacttgttc aagcatttg 
      aatgagaaa caccgagat cggttgatt gttgccgga 
      gaggtgcat attttggac ctgggggag ggggaggtc 
      atgaagacg tttgccatt tgcttcggg aatgatatg 
      ttccgattg gcggtgttt tacgatggg gatattaaa 
      ttctcgttg caacagttt ggggttaaa caaagttgt 
      tacacttat gagttggag attatttgt acgacggat 
      tgttccgag aagattatc ttgacgaag gagtgtcca 
      cccatgagt acgaccttc tggtccaag agcattacg 
      gtcccggat gatttgata caaaggttc atctcggag 
      gaggatttg gtctttttt aaaatcaat ttgaggaaa 
      atggtttag ttattcttc agtgccaat tatttatct 
      gtgttacta ttgtatgga acggctcat ttttgttta 
      ataaatttg tattctttt g