Status
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Gene information
General information
TC003272 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 608773..625797
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003272
    iBeetle screen iB_02683

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003272 RA
      MGVNFVWFR HKKRFFACG HFLPFPGRF YSASRKADL 
      GDAYKPGVV ESEERECCD YFRPKARSS QTFSMILPP 
      PNITGSLHL GHALTATVQ DVIVRWKRM EGVDCVWVP 
      GIDHAGIAT QVVVEKLLW GEKGQSRHD LGRERFQAR 
      VWNWKEEKE AVIESQLRR LGACLDWSR KVFTMDPKQ 
      SQAVAQAFI KLFESGLIY RADHLVNWS CSLQSAISD 
      IEVDHLEVI KATKIAVPN CDKAVEFGT LTRFAYKVC 
      DSDEDEVVV ATTRPETML GDVAVAVNP RDGRYSRFV 
      GRFLWHPFR KVKIPVICD DFVDPEFGT GAVKVTPAH 
      DPVDFEVGK RHSLALLQV IDEKGNLTR ICGEFAGIG 
      RFAAREVIL SRLEKLQLL RGREDHKML VPICSRSKD 
      IVEHLVKPQ WFIKCAEMA KEAVEDVES GRLTIEPKH 
      FEKVWFDWL GNIRDWCIS RQLWWGHRI PAFSCRPQD 
      GSAPAIWVP AMDQNSALR KASAHFSCP QLEIQQDED 
      VLDTWFSSA LLPFSVWRS QQESPNYPL DLMETGHDI 
      LFFWVARMV MLGKQLTGR LPFERVLLH GIIRDAHGR 
      KMSKSLGNV ILPEEVICG TTSEKLEEG SRLSHEAGI 
      LSKEELTKA LEGQKKMFP SGIPECGAD ALRFTLCSH 
      NIKSHFIDF DVQECHTNR LFCNKIWQA TKFTNNAVE 
      TAREWLGPI DTHQLGLMD RWILSKLSG MVDVCRQGM 
      HNHDFHQVT AAIKEFLYF QFCDVYLET TKRAFKLSE 
      RGNAAGHCW TLTHCLDTA LRALAPFMP RLAQHLHQR 
      LETYPGTER NFYYPKDLN LRDVKLEED VSKVMEVVV 
      GIRRLKKLF GVAAKHKPE VYLLGDGSF FAPFLPVME 
      ALSICPLVT LAPPPKDTV CDRVGDTHI HLVVPPELR 
      KAFELDLPK LEDKKTKLV KELAKMNKM LSGENYKLK 
      ATPEAQKSH AKKIAVLEE KLARINHIQ SLSKS
    CDS sequence
    • >TC003272 RA
      atgggtgtt aattttgtg tggtttcga cacaaaaaa 
      cggtttttt gcatgtggg cattttttg ccgtttccc 
      ggtcgattt tacagcgca tctaggaag gccgacctg 
      ggggatgcc tacaagccg ggtgttgtg gaatctgaa 
      gagagggaa tgttgtgac tacttcagg cctaaggct 
      aggtcaagt cagacgttt agtatgatt ttgcctccg 
      ccaaatatc accgggagt cttcatttg gggcacgcg 
      ttgactgcg accgtccag gatgtgata gtgcgctgg 
      aaacgtatg gagggtgtg gattgtgtc tgggtgccg 
      gggattgac catgcaggg attgccacc caagtggtg 
      gtagagaag ctcctctgg ggcgaaaag ggccaaagt 
      aggcacgat ttagggcgc gagcggttc caggctcgt 
      gtctggaac tggaaggag gagaaagaa gccgtgata 
      gaaagtcag ttgcgtcgt ttgggggct tgtctggac 
      tggtctcgc aaagtcttc accatggac ccaaagcag 
      tcgcaagcc gttgcacaa gcttttatt aaattgttc 
      gagtctggg ttaatatac cgggcggac catttggtg 
      aattggtca tgtagtctc caatcggcc atctccgat 
      atagaagtg gaccacttg gaggtgatc aaagccacc 
      aagatcgca gtgcccaac tgtgacaaa gctgtcgag 
      tttggaact ttgactcgt tttgcttat aaagtttgc 
      gattcggac gaggacgaa gttgtggtg gctacaact 
      cggcctgag actatgttg ggggacgtg gctgtggcg 
      gtgaatccc cgagatgga cgatatagt cgatttgtt 
      gggcggttt ttatggcat ccgttccga aaggtgaaa 
      attccggtg atttgtgac gattttgtc gatccggag 
      ttcggaact ggtgccgtg aaggtgacc ccagcccac 
      gaccccgtg gacttcgag gtgggcaaa cggcactcg 
      ctggccctc cttcaagtg atcgatgaa aagggcaat 
      ctcactcga atctgtggc gaattcgcc ggaatcgga 
      agattcgca gcgagagaa gtaatatta tcgcgcttg 
      gaaaagttg caactgctg agaggcaga gaggaccac 
      aagatgctt gttccaatt tgcagccgc tccaaggac 
      attgtcgag cacctggtg aagcctcag tggttcatc 
      aagtgcgcg gaaatggct aaagaggcc gtcgaggac 
      gtggaaagc ggccgattg acgattgaa ccgaaacat 
      ttcgagaag gtctggttc gattggctt ggaaatatc 
      agagactgg tgtatctcg cgccagctc tggtggggc 
      caccgcatc cctgccttc tcctgcaga cctcaggac 
      ggctcagcc cctgcgata tgggtccca gcaatggac 
      caaaactca gcccttcgc aaagcctca gcccacttc 
      tcctgccct cagctcgaa atccagcag gacgaagat 
      gtccttgac acctggttc tcatcagcc cttctcccc 
      ttctcagta tggcgctcg caacaggag tccccgaat 
      tatcccctg gatttgatg gaaaccggt cacgacatc 
      ctgtttttc tgggtggct cgtatggtc atgttgggc 
      aaacagctc acggggcgg ctgcccttt gagcgggtg 
      ctcctgcat gggatcatc cgggacgcc cacggcagg 
      aagatgtcg aagagtttg gggaacgtg atcttgccc 
      gaggaggtg atttgcggg acgacgtca gagaaattg 
      gaagagggg tcgaggttg agtcatgag gctgggatt 
      ttatcgaag gaggagttg actaaagcg ctcgaaggt 
      cagaagaag atgtttcct tccgggatt ccggagtgc 
      ggagcggat gcgcttaga tttacgctt tgttcgcac 
      aatattaag agccatttc atcgatttc gacgtgcaa 
      gagtgtcac acgaacaga ttgttctgc aacaagatc 
      tggcaagcg accaaattt accaataat gcagtggag 
      acggcgagg gaatggctg gggcccata gacacccat 
      caactgggg ctaatggat cgctggatt ctgagcaaa 
      ttgagcgga atggtggat gtgtgccgg caagggatg 
      cataatcac gacttccat caagtcacg gcggcgatc 
      aaggagttt ctctacttc cagttttgc gacgtctat 
      ctggagacg accaagagg gctttcaaa ctgtcagag 
      agaggaaat gccgccgga cattgctgg accttgacg 
      cattgcctc gatacggcc ctaagggcc cttgcaccc 
      tttatgcca cgactggcc caacatttg caccaaaga 
      cttgaaacc tatcctggc actgaacgc aatttttac 
      taccctaaa gatttaaat ttgcgagac gtgaagtta 
      gaagaggac gtttcgaaa gtgatggaa gtggttgtg 
      gggatcaga cggttgaag aaactcttc ggagttgcc 
      gccaaacac aaacctgaa gtttatttg ttgggggat 
      gggtcattt ttcgccccc ttcctcccc gtgatggaa 
      gccctctcc atctgccct ttggtaacg cttgcacca 
      cctcccaaa gacacagtt tgtgaccgc gttggagac 
      actcacatt cacctagtc gttcctcca gaattacgc 
      aaagctttc gaattagat ttgccaaaa ttagaggac 
      aagaagacg aaattagtc aaggaattg gccaaaatg 
      aacaaaatg ctctcgggg gagaattac aagcttaaa 
      gcaacccct gaagctcag aaatctcat gctaagaag 
      attgccgtt ttggaggag aaattggct cggatcaat 
      cacattcag agtctttcg aaatcgtag
    mRNA sequence
    • >TC003272 RA
      attgagtga aaatgggtg ttaattttg tgtggtttc 
      gacacaaaa aacggtttt ttgcatgtg ggcattttt 
      tgccgtttc ccggtcgat tttacagcg catctagga 
      aggccgacc tgggggatg cctacaagc cgggtgttg 
      tggaatctg aagagaggg aatgttgtg actacttca 
      ggcctaagg ctaggtcaa gtcagacgt ttagtatga 
      ttttgcctc cgccaaata tcaccggga gtcttcatt 
      tggggcacg cgttgactg cgaccgtcc aggatgtga 
      tagtgcgct ggaaacgta tggagggtg tggattgtg 
      tctgggtgc cggggattg accatgcag ggattgcca 
      cccaagtgg tggtagaga agctcctct ggggcgaaa 
      agggccaaa gtaggcacg atttagggc gcgagcggt 
      tccaggctc gtgtctgga actggaagg aggagaaag 
      aagccgtga tagaaagtc agttgcgtc gtttggggg 
      cttgtctgg actggtctc gcaaagtct tcaccatgg 
      acccaaagc agtcgcaag ccgttgcac aagctttta 
      ttaaattgt tcgagtctg ggttaatat accgggcgg 
      accatttgg tgaattggt catgtagtc tccaatcgg 
      ccatctccg atatagaag tggaccact tggaggtga 
      tcaaagcca ccaagatcg cagtgccca actgtgaca 
      aagctgtcg agtttggaa ctttgactc gttttgctt 
      ataaagttt gcgattcgg acgaggacg aagttgtgg 
      tggctacaa ctcggcctg agactatgt tgggggacg 
      tggctgtgg cggtgaatc cccgagatg gacgatata 
      gtcgatttg ttgggcggt ttttatggc atccgttcc 
      gaaaggtga aaattccgg tgatttgtg acgattttg 
      tcgatccgg agttcggaa ctggtgccg tgaaggtga 
      ccccagccc acgaccccg tggacttcg aggtgggca 
      aacggcact cgctggccc tccttcaag tgatcgatg 
      aaaagggca atctcactc gaatctgtg gcgaattcg 
      ccggaatcg gaagattcg cagcgagag aagtaatat 
      tatcgcgct tggaaaagt tgcaactgc tgagaggca 
      gagaggacc acaagatgc ttgttccaa tttgcagcc 
      gctccaagg acattgtcg agcacctgg tgaagcctc 
      agtggttca tcaagtgcg cggaaatgg ctaaagagg 
      ccgtcgagg acgtggaaa gcggccgat tgacgattg 
      aaccgaaac atttcgaga aggtctggt tcgattggc 
      ttggaaata tcagagact ggtgtatct cgcgccagc 
      tctggtggg gccaccgca tccctgcct tctcctgca 
      gacctcagg acggctcag cccctgcga tatgggtcc 
      cagcaatgg accaaaact cagcccttc gcaaagcct 
      cagcccact tctcctgcc ctcagctcg aaatccagc 
      aggacgaag atgtccttg acacctggt tctcatcag 
      cccttctcc ccttctcag tatggcgct cgcaacagg 
      agtccccga attatcccc tggatttga tggaaaccg 
      gtcacgaca tcctgtttt tctgggtgg ctcgtatgg 
      tcatgttgg gcaaacagc tcacggggc ggctgccct 
      ttgagcggg tgctcctgc atgggatca tccgggacg 
      cccacggca ggaagatgt cgaagagtt tggggaacg 
      tgatcttgc ccgaggagg tgatttgcg ggacgacgt 
      cagagaaat tggaagagg ggtcgaggt tgagtcatg 
      aggctggga ttttatcga aggaggagt tgactaaag 
      cgctcgaag gtcagaaga agatgtttc cttccggga 
      ttccggagt gcggagcgg atgcgctta gatttacgc 
      tttgttcgc acaatatta agagccatt tcatcgatt 
      tcgacgtgc aagagtgtc acacgaaca gattgttct 
      gcaacaaga tctggcaag cgaccaaat ttaccaata 
      atgcagtgg agacggcga gggaatggc tggggccca 
      tagacaccc atcaactgg ggctaatgg atcgctgga 
      ttctgagca aattgagcg gaatggtgg atgtgtgcc 
      ggcaaggga tgcataatc acgacttcc atcaagtca 
      cggcggcga tcaaggagt ttctctact tccagtttt 
      gcgacgtct atctggaga cgaccaaga gggctttca 
      aactgtcag agagaggaa atgccgccg gacattgct 
      ggaccttga cgcattgcc tcgatacgg ccctaaggg 
      cccttgcac cctttatgc cacgactgg cccaacatt 
      tgcaccaaa gacttgaaa cctatcctg gcactgaac 
      gcaattttt actacccta aagatttaa atttgcgag 
      acgtgaagt tagaagagg acgtttcga aagtgatgg 
      aagtggttg tggggatca gacggttga agaaactct 
      tcggagttg ccgccaaac acaaacctg aagtttatt 
      tgttggggg atgggtcat ttttcgccc ccttcctcc 
      ccgtgatgg aagccctct ccatctgcc ctttggtaa 
      cgcttgcac cacctccca aagacacag tttgtgacc 
      gcgttggag acactcaca ttcacctag tcgttcctc 
      cagaattac gcaaagctt tcgaattag atttgccaa 
      aattagagg acaagaaga cgaaattag tcaaggaat 
      tggccaaaa tgaacaaaa tgctctcgg gggagaatt 
      acaagctta aagcaaccc ctgaagctc agaaatctc 
      atgctaaga agattgccg ttttggagg agaaattgg 
      ctcggatca atcacattc agagtcttt cgaaatcgt 
      agtaattta agttaattt tgtacgaaa taaattgtt 
      tccatttgg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY