| General information | |||
| TC003186 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 566791..575048 | |
| Sequence information | |||
| Transcripts / Proteins (5) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC003186 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02682 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)Days post injection
Fertility
ovary without detectable defects -
Number of animals showing the phenotype: 4
Analysis of embryonic musculature and early development
number of eggs/embryos/larvae on slide: < 50
embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) -
Penetrance (percentage of animals/eggs on slide): less than 30%
larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larval musculature sometimes thinner - Penetrance (percentage of animals/eggs on slide): less than 30%
larval musculature sometimes partiallynot present - Penetrance (percentage of animals/eggs on slide): less than 30%
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
remarks: muscle pattern potentially obscured by segmentation defect,
larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larval musculature sometimes thinner - Penetrance (percentage of animals/eggs on slide): less than 30%
larval musculature sometimes partiallynot present - Penetrance (percentage of animals/eggs on slide): less than 30%
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
remarks: muscle pattern potentially obscured by segmentation defect,
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 50
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 2 (11 dpi)
Days post injection
pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
Days post injection
pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
Days post injection
pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 3 (16 dpi)
Days post injection
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (16 dpi)
Days post injection
- >TC003186 RA
MKIWCAAGV NLMGGYTKD GGLMVGGSV FYSEEPRLE QPHTELETL DQELNAGRE GALLETKLS SLVWICTYT HAASLVTVI DANNPAEIL NSFGVCANH VLCIASVPG ASPNDYNKT YTKINVSEN AKDSSDNVV ENKEDEGEN NTNKTNDKN HEELEEAAL IGKVTFVEN DTSVVNKKD KYNNAKSTV DEEKIEEQP VEDETDENV GESDKNKTS NQEIMSSVL ATMWLGAEN GMVYVHSSV ANWNQCLHS VKLKDAVIS IVHINGRVV CALACGTLA IFARNDDGE WDLSRYHLV QVGLPHQSV RCLAVVGDK VWCGYRNKI HVLEPQELG IIHSLEAHP RRESPVRQI AWLGEGVWV SIRLDSTLR LYHAHTYQH LQDVDIEPY VSKMLGTGK LGFSFVRIT CLLISSNRL WIGTSNGVI ISVPLSENG ANTGSIVPR GSTSLPGAG VRVAPVPGS FIPFCTMAH AQLSFHGHR DNVKFFVAV PGNGGMSAA STPSEALST SATSIGLPP KQPSAMLVM SGGEGYIDF RVGDEMEDS VIDTETGDL EAAQGESKG EKSHLIVWQ VSTS
- >TC003186 RB
MDLADSGGG LGPETIYGT HEDSHVVMS EKVQSLAGS IYQEFERMI ARYDEDVVK TLMPLLVNV LECLDAAYQ QNQEHDVEL ELLREDNEQ LVTQYEREK GARKAAEQK LLEFEDAAE GERKELAAR LEALESIVR MLELKHKNS MEHAGRLEE REGELKKEY AKLHERYTE LFKTHMDYM ERTKLLLSG QQLANERAE AGRLNSNRI NIGRSSGPI SFGFTSLEN AEVVDSVPS SPISSTHSS PSLHSELDN LDKKMVERG QETDSLNLE NKSVITSPV SPPGQPTTN NNTGRLKKE QRSGNTLYQ ELSFQDVDG DDDGDITGG WVHPGEYAS SVNDNFYGM GKEVENLIM ENNELLATK NALNVVKDD LIVKVDELT GEIEMLREE ILSLNVSRT KLRERISDL EEELRKVKE ANEAKQDGA DDESDVPMA QRKRFTRVE MARVLMERN QYKERFMEL QEAVRWTEM IRASRNDTA FDKKSKQSI WRFFSNLFS GSERPQRPL VGPHLRYQT ATNQIAPGV RALPRPGHD FVETELGTE V
- >TC003186 RC
MDLADSGGG LGPETIYGT HEDSHVVMS EKVQSLAGS IYQEFERMI ARYDEDVVK TLMPLLVNV LECLDAAYQ QNQEHDVEL ELLREDNEQ LVTQYEREK GARKAAEQK LLEFEDAAE GERKELAAR LEALESIVR MLELKHKNS MEHAGRLEE REGELKKEY AKLHERYTE LFKTHMDYM ERTKLLLSG QQLANERAE AGRLNSNRI NIGRSSGPI SFGFTSLEN AEVVDSVPS SPISSTHSS PSLHSELDN LDKKMVERG QETDSLNLE NKSVITSPV SPPGQPTTN NNTGRLKKE QRSGNTLYQ ELSFQDVDG DDDGDITGG WVHPGEYAS SGMGKEVEN LIMENNELL ATKNALNVV KDDLIVKVD ELTGEIEML REEILSLNV SRTKLRERI SDLEEELRK VKEANEAKQ DGADDESDV PMAQRKRFT RVEMARVLM ERNQYKERF MELQEAVRW TEMIRASRN DTAFDKKSK QSIWRFFSN LFSGSERPQ RPLVGPHLR YQTATNQIA PGVRALPRP GHDFVETEL GTEV
- >TC003186 RD
MDLADSGGG LGPETIYGT HEDSHVVMS EKVQSLAGS IYQEFERMI ARYDEDVVK TLMPLLVNV LECLDAAYQ QNQEHDVEL ELLREDNEQ LVTQYEREK GARKAAEQK LLEFEDAAE GERKELAAR LEALESIVR MLELKHKNS MEHAGRLEE REGELKKEY AKLHERYTE LFKTHMDYM ERTKLLLSG QQLANERAE AGRLNSNRI NIGRSSGPI SFGFTSLEN AEVVDSVPS SPISSTHSS PSLHSELDN LDKKMVERG QETDSLNLE NKSVITSPV SPPGQPTTN NNTGRLKKE QRSGNTLYQ ELSFQDVDG DDDGDITGG WVHPGEYAS SVNDNFYGM GKEVENLIM ENNELLATK NALNVVKDD LIVKVDELT GEIEMLREE ILSLNVSRT KLRERISDL EEELRKVKE ANEAKQDGA DDESDVPMA QRKRFTRVE MARVLMERN QYKERFMEL QEAVRWTEM IRASRNDTA FDKKSKQSI WRFFSNLFS GSERPQRPL VGPHLRYQT ATNQIAPGV RALPRPGHD FVETELGSE KLALRRAVE RREQYRQVR AHVRKEDGR LQAYGWSLP GKVGPGTKG TQSSSGVPV PVPVYCRPL AETTPGMKI WCAAGVNLM GGYTKDGGL MVGGSVFYS EEPRLEQPH TELETLDQE LNAGREGAL LETKLSSLV WICTYTHAA SLVTVIDAN NPAEILNSF GVCANHVLC IASVPGASP NDYNKTYTK INVSENAKD SSDNVVENK EDEGENNTN KTNDKNHEE LEEAALIGK VTFVENDTS VVNKKDKYN NAKSTVDEE KIEEQPVED ETDENVGES DKNKTSNQE IMSSVLATM WLGAENGMV YVHSSVANW NQCLHSVKL KDAVISIVH INGRVVCAL ACGTLAIFA RNDDGEWDL SRYHLVQVG LPHQSVRCL AVVGDKVWC GYRNKIHVL EPQELGIIH SLEAHPRRE SPVRQIAWL GEGVWVSIR LDSTLRLYH AHTYQHLQD VDIEPYVSK MLGTGKLGF SFVRITCLL ISSNRLWIG TSNGVIISV PLSENGANT GSIVPRGST SLPGAGVRV APVPGSFIP FCTMAHAQL SFHGHRDNV KFFVAVPGN GGMSAASTP SEALSTSAT SIGLPPKQP SAMLVMSGG EGYIDFRVE DENEPRDGD SHLLVWQVL PDIPGNPVG
- >TC003186 RE
MKIWCAAGV NLMGGYTKD GGLMVGGSV FYSEEPRLE QPHTELETL DQELNAGRE GALLETKLS SLVWICTYT HAASLVTVI DANNPAEIL NSFGVCANH VLCIASVPG ASPNDYNKT YTKINVSEN AKDSSDNVV ENKEDEGEN NTNKTNDKN HEELEEAAL IGKVTFVEN DTSVVNKKD KYNNAKSTV DEEKIEEQP VEDETDENV GESDKNKTS NQEIMSSVL ATMWLGAEN GMVYVHSSV ANWNQCLHS VKLKDAVIS IVHINGRVV CALACGTLA IFARNDDGE WDLSRYHLV QVGLPHQSV RCLAVVGDK VWCGYRNKI HVLEPQELG IIHSLEAHP RRESPVRQI AWLGEGVWV SIRLDSTLR LYHAHTYQH LQDVDIEPY VSKMLGTGK LGFSFVRIT CLLISSNRL WIGTSNGVI ISVPLSENG ANTGSIVPR GSTSLPGAG VRVAPVPGS FIPFCTMAH AQLSFHGHR DNVKFFVAV PGNGGMSAA STPSEALST SATSIGLPP KQPSAMLVM SGGEGYIDF RVEDENEPR DGDSHLLVW QVLPDIPGN PVG
- >TC003186 RA
atgaagatt tggtgtgca gcgggtgtt aatctcatg gggggttac accaaagat ggggggctt atggtgggg ggcagtgtc ttctactcg gaagagccc cgcctggag cagccccac accgagcta gagactctg gatcaggag ctgaatgca ggtcgtgaa ggggcactt ctcgagaca aaattatcg tcacttgtg tggatttgt acatacaca catgctgct agtttagta accgtaatc gatgcgaat aatccagcg gagattttg aatagtttt ggggtttgt gcaaaccat gttttatgc attgctagt gtacctggt gctagtcca aacgattat aataaaacg tataccaaa attaacgtt agtgagaat gcaaaagac agcagtgat aacgtcgta gaaaataaa gaagacgaa ggggaaaat aatacaaac aaaacgaat gataaaaat cacgaagag ttggaagag gcggctttg attgggaaa gttacgttt gttgaaaac gatacttct gtggttaat aaaaaagac aaatataat aatgcaaaa tcaacggtg gatgaagag aaaattgag gaacaacct gttgaggat gaaactgat gaaaatgtg ggtgaaagt gataagaat aaaacgagc aatcaggaa ataatgtcg agtgttttg gcaacaatg tggctagga gcggaaaat ggaatggtt tatgttcac tcatcggtg gccaattgg aatcagtgt ctgcattcc gtcaaactt aaagatgca gtgataagt attgtacat ataaatggc cgtgtcgtt tgtgctttg gcttgtggt actttggca attttcgca cgaaacgat gatggagaa tgggacttg tcccgttac catttggta caagtgggt ttaccacat cagtccgtt cgttgtttg gctgttgtt ggagataaa gtctggtgc ggatatagg aataaaatt cacgtgctt gaaccacaa gaattggga ataattcat tcgctggaa gcgcatcct cgacgtgaa agtcccgtg cgacaaatt gcgtggttg ggtgaaggt gtttgggtt tcgattcgt ttagattcg actttaaga ctgtaccat gctcatacc taccagcat ttgcaggat gtggatatt gagccgtac gtcagcaag atgttgggt actggaaag ctgggcttt tccttcgtg cgcataacg tgtcttttg atatcgtca aaccgcctc tggattggc acaagcaac ggtgtcata atttccgtt ccattgagc gagaacggg gcaaacacc ggaagcata gttccccgg ggatcaacc agtcttccc ggagcggga gtacgtgtt gctcccgtt cccggaagc tttatccct ttttgtacc atggcccac gctcaactc agcttccac ggccataga gataacgtg aaatttttt gtcgccgtc cccggaaac ggaggaatg agcgcggct tcgacgcct tcagaggca ctgtcgact tctgcgact tcgatcggg ttgcccccc aagcagccg tcggcaatg ctggtgatg tcggggggc gaaggttac atcgatttc cgcgtcggt gatgagatg gaggatagc gttattgat actgaaacc ggtgatctt gaagcagct cagggcgaa tccaaaggg gaaaaaagt cacttgatt gtgtggcaa gtgtccaca agttaa
- >TC003186 RB
atggatttg gctgactcc ggcggtggt cttggaccc gagaccatc tacgggacg cacgaggac tcccatgtg gtgatgtcg gagaaggtg caatcgttg gctggtagc atctaccag gagttcgaa cgaatgatc gcacgctac gacgaagac gtcgtcaag accctcatg cccctgctg gtgaacgtg ctggagtgt ttggacgcg gcgtatcaa cagaaccag gagcacgac gtggagctg gagctgctc cgcgaagac aacgagcag ttggtcacg cagtacgag cgcgagaag ggggcgcgc aaagccgcc gagcagaaa ctcctagag tttgaagac gccgccgaa ggggagcgc aaggagttg gccgcacgg ctcgaagcg ctggaaagc atcgtcagg atgctggag ctgaagcac aagaactcg atggagcat gcgggacgt ttggaggag cgtgaaggg gagttgaag aaggaatac gccaagttg cacgaacgc tacaccgag ttgttcaaa acacacatg gactacatg gagcggacg aagttgctg ctttctgga caacagctg gccaatgag cgggccgaa gccggccgt ttgaactcg aatcggatt aatataggg cgcagttcg gggcccatc tccttcggg ttcacctcc ttggagaat gccgaggtg gtggacagt gttccgtcg tcgccgatt agtagtacg cattcgtcg ccgagtttg cacagcgag ttggataat ttggacaag aagatggtg gaaaggggg caagagact gattcgctc aatttggaa aataaaagt gtgataaca tcgccggtg agtccccca ggtcaaccg acaactaac aataatacg gggaggttg aagaaggaa caaaggagt gggaacacg ttgtatcaa gagttgagt tttcaggat gtggatggc gatgatgat ggtgacata accggaggt tgggtccat ccaggtgaa tacgcttca tcagtcaac gataatttt tacggaatg ggcaaagaa gtcgaaaat ttgataatg gaaaacaac gagctacta gccaccaaa aacgccctt aatgtggta aaagacgac cttatcgta aaagttgac gaattaacc ggcgaaatt gaaatgctt cgcgaagaa atcctctcg ttaaatgtc tcacgaacg aaactccgt gaaagaatc agtgatctc gaagaagaa ttgcgaaaa gttaaggaa gcaaatgaa gcgaaacaa gacggtgcc gatgatgaa tctgatgtg ccaatggct caaaggaag cgtttcacg agagtggaa atggcacgt gttctcatg gagcgcaac caatacaaa gagcgcttc atggagttg caagaagcc gtccgttgg acggagatg attcgagcg tcccgtaac gacaccgcc tttgacaaa aagagcaaa cagagcatt tggaggttc tttagtaat ttgtttagt ggttcggag cggccgcaa agaccattg gttggtcca catctacgt taccagacg gcgaccaat caaatagcg ccaggtgtt agagccctg cctcgaccc ggtcatgac tttgtcgaa actgaattg ggtacggaa gtgtga
- >TC003186 RC
atggatttg gctgactcc ggcggtggt cttggaccc gagaccatc tacgggacg cacgaggac tcccatgtg gtgatgtcg gagaaggtg caatcgttg gctggtagc atctaccag gagttcgaa cgaatgatc gcacgctac gacgaagac gtcgtcaag accctcatg cccctgctg gtgaacgtg ctggagtgt ttggacgcg gcgtatcaa cagaaccag gagcacgac gtggagctg gagctgctc cgcgaagac aacgagcag ttggtcacg cagtacgag cgcgagaag ggggcgcgc aaagccgcc gagcagaaa ctcctagag tttgaagac gccgccgaa ggggagcgc aaggagttg gccgcacgg ctcgaagcg ctggaaagc atcgtcagg atgctggag ctgaagcac aagaactcg atggagcat gcgggacgt ttggaggag cgtgaaggg gagttgaag aaggaatac gccaagttg cacgaacgc tacaccgag ttgttcaaa acacacatg gactacatg gagcggacg aagttgctg ctttctgga caacagctg gccaatgag cgggccgaa gccggccgt ttgaactcg aatcggatt aatataggg cgcagttcg gggcccatc tccttcggg ttcacctcc ttggagaat gccgaggtg gtggacagt gttccgtcg tcgccgatt agtagtacg cattcgtcg ccgagtttg cacagcgag ttggataat ttggacaag aagatggtg gaaaggggg caagagact gattcgctc aatttggaa aataaaagt gtgataaca tcgccggtg agtccccca ggtcaaccg acaactaac aataatacg gggaggttg aagaaggaa caaaggagt gggaacacg ttgtatcaa gagttgagt tttcaggat gtggatggc gatgatgat ggtgacata accggaggt tgggtccat ccaggtgaa tacgcttca tcaggaatg ggcaaagaa gtcgaaaat ttgataatg gaaaacaac gagctacta gccaccaaa aacgccctt aatgtggta aaagacgac cttatcgta aaagttgac gaattaacc ggcgaaatt gaaatgctt cgcgaagaa atcctctcg ttaaatgtc tcacgaacg aaactccgt gaaagaatc agtgatctc gaagaagaa ttgcgaaaa gttaaggaa gcaaatgaa gcgaaacaa gacggtgcc gatgatgaa tctgatgtg ccaatggct caaaggaag cgtttcacg agagtggaa atggcacgt gttctcatg gagcgcaac caatacaaa gagcgcttc atggagttg caagaagcc gtccgttgg acggagatg attcgagcg tcccgtaac gacaccgcc tttgacaaa aagagcaaa cagagcatt tggaggttc tttagtaat ttgtttagt ggttcggag cggccgcaa agaccattg gttggtcca catctacgt taccagacg gcgaccaat caaatagcg ccaggtgtt agagccctg cctcgaccc ggtcatgac tttgtcgaa actgaattg ggtacggaa gtgtga
- >TC003186 RD
atggatttg gctgactcc ggcggtggt cttggaccc gagaccatc tacgggacg cacgaggac tcccatgtg gtgatgtcg gagaaggtg caatcgttg gctggtagc atctaccag gagttcgaa cgaatgatc gcacgctac gacgaagac gtcgtcaag accctcatg cccctgctg gtgaacgtg ctggagtgt ttggacgcg gcgtatcaa cagaaccag gagcacgac gtggagctg gagctgctc cgcgaagac aacgagcag ttggtcacg cagtacgag cgcgagaag ggggcgcgc aaagccgcc gagcagaaa ctcctagag tttgaagac gccgccgaa ggggagcgc aaggagttg gccgcacgg ctcgaagcg ctggaaagc atcgtcagg atgctggag ctgaagcac aagaactcg atggagcat gcgggacgt ttggaggag cgtgaaggg gagttgaag aaggaatac gccaagttg cacgaacgc tacaccgag ttgttcaaa acacacatg gactacatg gagcggacg aagttgctg ctttctgga caacagctg gccaatgag cgggccgaa gccggccgt ttgaactcg aatcggatt aatataggg cgcagttcg gggcccatc tccttcggg ttcacctcc ttggagaat gccgaggtg gtggacagt gttccgtcg tcgccgatt agtagtacg cattcgtcg ccgagtttg cacagcgag ttggataat ttggacaag aagatggtg gaaaggggg caagagact gattcgctc aatttggaa aataaaagt gtgataaca tcgccggtg agtccccca ggtcaaccg acaactaac aataatacg gggaggttg aagaaggaa caaaggagt gggaacacg ttgtatcaa gagttgagt tttcaggat gtggatggc gatgatgat ggtgacata accggaggt tgggtccat ccaggtgaa tacgcttca tcagtcaac gataatttt tacggaatg ggcaaagaa gtcgaaaat ttgataatg gaaaacaac gagctacta gccaccaaa aacgccctt aatgtggta aaagacgac cttatcgta aaagttgac gaattaacc ggcgaaatt gaaatgctt cgcgaagaa atcctctcg ttaaatgtc tcacgaacg aaactccgt gaaagaatc agtgatctc gaagaagaa ttgcgaaaa gttaaggaa gcaaatgaa gcgaaacaa gacggtgcc gatgatgaa tctgatgtg ccaatggct caaaggaag cgtttcacg agagtggaa atggcacgt gttctcatg gagcgcaac caatacaaa gagcgcttc atggagttg caagaagcc gtccgttgg acggagatg attcgagcg tcccgtaac gacaccgcc tttgacaaa aagagcaaa cagagcatt tggaggttc tttagtaat ttgtttagt ggttcggag cggccgcaa agaccattg gttggtcca catctacgt taccagacg gcgaccaat caaatagcg ccaggtgtt agagccctg cctcgaccc ggtcatgac tttgtcgaa actgaattg ggttcggaa aagttggct ttgaggagg gctgttgag agacgagaa cagtaccga caagtgaga gcgcatgtg cgaaaagag gatggaaga ttgcaagcg tatggttgg agtttgccc ggaaaagtt ggtccggga acgaaagga acacaaagt agttccggg gttccggtc ccggtcccg gtttattgt aggccattg gctgaaact acgcccggg atgaagatt tggtgtgca gcgggtgtt aatctcatg gggggttac accaaagat ggggggctt atggtgggg ggcagtgtc ttctactcg gaagagccc cgcctggag cagccccac accgagcta gagactctg gatcaggag ctgaatgca ggtcgtgaa ggggcactt ctcgagaca aaattatcg tcacttgtg tggatttgt acatacaca catgctgct agtttagta accgtaatc gatgcgaat aatccagcg gagattttg aatagtttt ggggtttgt gcaaaccat gttttatgc attgctagt gtacctggt gctagtcca aacgattat aataaaacg tataccaaa attaacgtt agtgagaat gcaaaagac agcagtgat aacgtcgta gaaaataaa gaagacgaa ggggaaaat aatacaaac aaaacgaat gataaaaat cacgaagag ttggaagag gcggctttg attgggaaa gttacgttt gttgaaaac gatacttct gtggttaat aaaaaagac aaatataat aatgcaaaa tcaacggtg gatgaagag aaaattgag gaacaacct gttgaggat gaaactgat gaaaatgtg ggtgaaagt gataagaat aaaacgagc aatcaggaa ataatgtcg agtgttttg gcaacaatg tggctagga gcggaaaat ggaatggtt tatgttcac tcatcggtg gccaattgg aatcagtgt ctgcattcc gtcaaactt aaagatgca gtgataagt attgtacat ataaatggc cgtgtcgtt tgtgctttg gcttgtggt actttggca attttcgca cgaaacgat gatggagaa tgggacttg tcccgttac catttggta caagtgggt ttaccacat cagtccgtt cgttgtttg gctgttgtt ggagataaa gtctggtgc ggatatagg aataaaatt cacgtgctt gaaccacaa gaattggga ataattcat tcgctggaa gcgcatcct cgacgtgaa agtcccgtg cgacaaatt gcgtggttg ggtgaaggt gtttgggtt tcgattcgt ttagattcg actttaaga ctgtaccat gctcatacc taccagcat ttgcaggat gtggatatt gagccgtac gtcagcaag atgttgggt actggaaag ctgggcttt tccttcgtg cgcataacg tgtcttttg atatcgtca aaccgcctc tggattggc acaagcaac ggtgtcata atttccgtt ccattgagc gagaacggg gcaaacacc ggaagcata gttccccgg ggatcaacc agtcttccc ggagcggga gtacgtgtt gctcccgtt cccggaagc tttatccct ttttgtacc atggcccac gctcaactc agcttccac ggccataga gataacgtg aaatttttt gtcgccgtc cccggaaac ggaggaatg agcgcggct tcgacgcct tcagaggca ctgtcgact tctgcgact tcgatcggg ttgcccccc aagcagccg tcggcaatg ctggtgatg tcggggggc gaaggttac atcgatttc cgcgtcgag gacgaaaat gaaccgaga gatggagac tcgcatctg ctcgtttgg caggttttg cctgatatt cctgggaat cctgtgggg tga
- >TC003186 RE
atgaagatt tggtgtgca gcgggtgtt aatctcatg gggggttac accaaagat ggggggctt atggtgggg ggcagtgtc ttctactcg gaagagccc cgcctggag cagccccac accgagcta gagactctg gatcaggag ctgaatgca ggtcgtgaa ggggcactt ctcgagaca aaattatcg tcacttgtg tggatttgt acatacaca catgctgct agtttagta accgtaatc gatgcgaat aatccagcg gagattttg aatagtttt ggggtttgt gcaaaccat gttttatgc attgctagt gtacctggt gctagtcca aacgattat aataaaacg tataccaaa attaacgtt agtgagaat gcaaaagac agcagtgat aacgtcgta gaaaataaa gaagacgaa ggggaaaat aatacaaac aaaacgaat gataaaaat cacgaagag ttggaagag gcggctttg attgggaaa gttacgttt gttgaaaac gatacttct gtggttaat aaaaaagac aaatataat aatgcaaaa tcaacggtg gatgaagag aaaattgag gaacaacct gttgaggat gaaactgat gaaaatgtg ggtgaaagt gataagaat aaaacgagc aatcaggaa ataatgtcg agtgttttg gcaacaatg tggctagga gcggaaaat ggaatggtt tatgttcac tcatcggtg gccaattgg aatcagtgt ctgcattcc gtcaaactt aaagatgca gtgataagt attgtacat ataaatggc cgtgtcgtt tgtgctttg gcttgtggt actttggca attttcgca cgaaacgat gatggagaa tgggacttg tcccgttac catttggta caagtgggt ttaccacat cagtccgtt cgttgtttg gctgttgtt ggagataaa gtctggtgc ggatatagg aataaaatt cacgtgctt gaaccacaa gaattggga ataattcat tcgctggaa gcgcatcct cgacgtgaa agtcccgtg cgacaaatt gcgtggttg ggtgaaggt gtttgggtt tcgattcgt ttagattcg actttaaga ctgtaccat gctcatacc taccagcat ttgcaggat gtggatatt gagccgtac gtcagcaag atgttgggt actggaaag ctgggcttt tccttcgtg cgcataacg tgtcttttg atatcgtca aaccgcctc tggattggc acaagcaac ggtgtcata atttccgtt ccattgagc gagaacggg gcaaacacc ggaagcata gttccccgg ggatcaacc agtcttccc ggagcggga gtacgtgtt gctcccgtt cccggaagc tttatccct ttttgtacc atggcccac gctcaactc agcttccac ggccataga gataacgtg aaatttttt gtcgccgtc cccggaaac ggaggaatg agcgcggct tcgacgcct tcagaggca ctgtcgact tctgcgact tcgatcggg ttgcccccc aagcagccg tcggcaatg ctggtgatg tcggggggc gaaggttac atcgatttc cgcgtcgag gacgaaaat gaaccgaga gatggagac tcgcatctg ctcgtttgg caggttttg cctgatatt cctgggaat cctgtgggg tga
- >TC003186 RA
aaaaaatta tttgaaaaa aattgtagg ttcggaaaa gttggcttt gaggagggc tgttgagag acgagaaca gtaccgaca agtgagagc gcatgtgcg aaaagagga tggaagatt gcaagcgta tggttggag tttgcccgg aaaagttgg tccgggaac gaaaggaac acaaagtag ttccggggt tccggtccc ggtcccggt ttattgtag gccattggc tgaaactac gcccgggat gaagatttg gtgtgcagc gggtgttaa tctcatggg gggttacac caaagatgg ggggcttat ggtgggggg cagtgtctt ctactcgga agagccccg cctggagca gccccacac cgagctaga gactctgga tcaggagct gaatgcagg tcgtgaagg ggcacttct cgagacaaa attatcgtc acttgtgtg gatttgtac atacacaca tgctgctag tttagtaac cgtaatcga tgcgaataa tccagcgga gattttgaa tagttttgg ggtttgtgc aaaccatgt tttatgcat tgctagtgt acctggtgc tagtccaaa cgattataa taaaacgta taccaaaat taacgttag tgagaatgc aaaagacag cagtgataa cgtcgtaga aaataaaga agacgaagg ggaaaataa tacaaacaa aacgaatga taaaaatca cgaagagtt ggaagaggc ggctttgat tgggaaagt tacgtttgt tgaaaacga tacttctgt ggttaataa aaaagacaa atataataa tgcaaaatc aacggtgga tgaagagaa aattgagga acaacctgt tgaggatga aactgatga aaatgtggg tgaaagtga taagaataa aacgagcaa tcaggaaat aatgtcgag tgttttggc aacaatgtg gctaggagc ggaaaatgg aatggttta tgttcactc atcggtggc caattggaa tcagtgtct gcattccgt caaacttaa agatgcagt gataagtat tgtacatat aaatggccg tgtcgtttg tgctttggc ttgtggtac tttggcaat tttcgcacg aaacgatga tggagaatg ggacttgtc ccgttacca tttggtaca agtgggttt accacatca gtccgttcg ttgtttggc tgttgttgg agataaagt ctggtgcgg atataggaa taaaattca cgtgcttga accacaaga attgggaat aattcattc gctggaagc gcatcctcg acgtgaaag tcccgtgcg acaaattgc gtggttggg tgaaggtgt ttgggtttc gattcgttt agattcgac tttaagact gtaccatgc tcataccta ccagcattt gcaggatgt ggatattga gccgtacgt cagcaagat gttgggtac tggaaagct gggcttttc cttcgtgcg cataacgtg tcttttgat atcgtcaaa ccgcctctg gattggcac aagcaacgg tgtcataat ttccgttcc attgagcga gaacggggc aaacaccgg aagcatagt tccccgggg atcaaccag tcttcccgg agcgggagt acgtgttgc tcccgttcc cggaagctt tatcccttt ttgtaccat ggcccacgc tcaactcag cttccacgg ccatagaga taacgtgaa attttttgt cgccgtccc cggaaacgg aggaatgag cgcggcttc gacgccttc agaggcact gtcgacttc tgcgacttc gatcgggtt gccccccaa gcagccgtc ggcaatgct ggtgatgtc ggggggcga aggttacat cgatttccg cgtcggtga tgagatgga ggatagcgt tattgatac tgaaaccgg tgatcttga agcagctca gggcgaatc caaagggga aaaaagtca cttgattgt gtggcaagt gtccacaag ttaattaag caaaatgat atttaacat taattgaaa tactaaaca ttagcggtt ttagattag taacgttta taaaaaaaa caacttgga cgatatttt agcttgtga atatgttat tgatacata atattttat cgttgtttt atatctgtt acatgtaaa aactattta ttatgattt gtaaatttg agacgtgta tttaggaaa atttttatt taaatgtat cgtgcttac actgtaaat gaaacttat acaaactga atgcttttt attcaaata agacccact cacaaatgc gattgcatt gacgtttga ggttatgtt agtgttctt ttgatgcca tatgtcgca ttaaattgt tgataaatt tgtctattt ttcgcacat cttgggcaa tctccgttt tgaattgta aacac
- >TC003186 RB
gcagtggca aaaatgaca gttgttcct ccagcccga tttttgtca tcataaaca gtgggtgta acaaaaaat cgcacactt ttccatcac cagtgcccc cagaatgtg ccccacgtt gtaaaatca tcacaacac aatgttccc tcaagtgac aatgtgcta ttcttgatt gggcaaaag taggttgaa tagttggtt aaataatgg atttggctg actccggcg gtggtcttg gacccgaga ccatctacg ggacgcacg aggactccc atgtggtga tgtcggaga aggtgcaat cgttggctg gtagcatct accaggagt tcgaacgaa tgatcgcac gctacgacg aagacgtcg tcaagaccc tcatgcccc tgctggtga acgtgctgg agtgtttgg acgcggcgt atcaacaga accaggagc acgacgtgg agctggagc tgctccgcg aagacaacg agcagttgg tcacgcagt acgagcgcg agaaggggg cgcgcaaag ccgccgagc agaaactcc tagagtttg aagacgccg ccgaagggg agcgcaagg agttggccg cacggctcg aagcgctgg aaagcatcg tcaggatgc tggagctga agcacaaga actcgatgg agcatgcgg gacgtttgg aggagcgtg aaggggagt tgaagaagg aatacgcca agttgcacg aacgctaca ccgagttgt tcaaaacac acatggact acatggagc ggacgaagt tgctgcttt ctggacaac agctggcca atgagcggg ccgaagccg gccgtttga actcgaatc ggattaata tagggcgca gttcggggc ccatctcct tcgggttca cctccttgg agaatgccg aggtggtgg acagtgttc cgtcgtcgc cgattagta gtacgcatt cgtcgccga gtttgcaca gcgagttgg ataatttgg acaagaaga tggtggaaa gggggcaag agactgatt cgctcaatt tggaaaata aaagtgtga taacatcgc cggtgagtc ccccaggtc aaccgacaa ctaacaata atacgggga ggttgaaga aggaacaaa ggagtggga acacgttgt atcaagagt tgagttttc aggatgtgg atggcgatg atgatggtg acataaccg gaggttggg tccatccag gtgaatacg cttcatcag tcaacgata atttttacg gaatgggca aagaagtcg aaaatttga taatggaaa acaacgagc tactagcca ccaaaaacg cccttaatg tggtaaaag acgacctta tcgtaaaag ttgacgaat taaccggcg aaattgaaa tgcttcgcg aagaaatcc tctcgttaa atgtctcac gaacgaaac tccgtgaaa gaatcagtg atctcgaag aagaattgc gaaaagtta aggaagcaa atgaagcga aacaagacg gtgccgatg atgaatctg atgtgccaa tggctcaaa ggaagcgtt tcacgagag tggaaatgg cacgtgttc tcatggagc gcaaccaat acaaagagc gcttcatgg agttgcaag aagccgtcc gttggacgg agatgattc gagcgtccc gtaacgaca ccgcctttg acaaaaaga gcaaacaga gcatttgga ggttcttta gtaatttgt ttagtggtt cggagcggc cgcaaagac cattggttg gtccacatc tacgttacc agacggcga ccaatcaaa tagcgccag gtgttagag ccctgcctc gacccggtc atgactttg tcgaaactg aattgggta cggaagtgt gattatttt cttactatt tgtacctgc tgtcaatct gtgatatgt tactactag agtagatct gagcaaaaa tcgaaattt gggttggta gacctgtct gcttttggt ggtgcgttg gagtgcatg atttttttt taacccccg actgtaatg aaacacgga atgttcaat tatttttta tccatttat taatccaaa gttacggta actaatcta gtcttactc aatttgtta ccacaataa ttgttacgt ttgttacaa atacatttt aataatttc c
- >TC003186 RC
gcagtggca aaaatgaca gttgttcct ccagcccga tttttgtca tcataaaca gtgggtgta acaaaaaat cgcacactt ttccatcac cagtgcccc cagaatgtg ccccacgtt gtaaaatca tcacaacac aatgttccc tcaagtgac aatgtgcta ttcttgatt gggcaaaag taggttgaa tagttggtt aaataatgg atttggctg actccggcg gtggtcttg gacccgaga ccatctacg ggacgcacg aggactccc atgtggtga tgtcggaga aggtgcaat cgttggctg gtagcatct accaggagt tcgaacgaa tgatcgcac gctacgacg aagacgtcg tcaagaccc tcatgcccc tgctggtga acgtgctgg agtgtttgg acgcggcgt atcaacaga accaggagc acgacgtgg agctggagc tgctccgcg aagacaacg agcagttgg tcacgcagt acgagcgcg agaaggggg cgcgcaaag ccgccgagc agaaactcc tagagtttg aagacgccg ccgaagggg agcgcaagg agttggccg cacggctcg aagcgctgg aaagcatcg tcaggatgc tggagctga agcacaaga actcgatgg agcatgcgg gacgtttgg aggagcgtg aaggggagt tgaagaagg aatacgcca agttgcacg aacgctaca ccgagttgt tcaaaacac acatggact acatggagc ggacgaagt tgctgcttt ctggacaac agctggcca atgagcggg ccgaagccg gccgtttga actcgaatc ggattaata tagggcgca gttcggggc ccatctcct tcgggttca cctccttgg agaatgccg aggtggtgg acagtgttc cgtcgtcgc cgattagta gtacgcatt cgtcgccga gtttgcaca gcgagttgg ataatttgg acaagaaga tggtggaaa gggggcaag agactgatt cgctcaatt tggaaaata aaagtgtga taacatcgc cggtgagtc ccccaggtc aaccgacaa ctaacaata atacgggga ggttgaaga aggaacaaa ggagtggga acacgttgt atcaagagt tgagttttc aggatgtgg atggcgatg atgatggtg acataaccg gaggttggg tccatccag gtgaatacg cttcatcag gaatgggca aagaagtcg aaaatttga taatggaaa acaacgagc tactagcca ccaaaaacg cccttaatg tggtaaaag acgacctta tcgtaaaag ttgacgaat taaccggcg aaattgaaa tgcttcgcg aagaaatcc tctcgttaa atgtctcac gaacgaaac tccgtgaaa gaatcagtg atctcgaag aagaattgc gaaaagtta aggaagcaa atgaagcga aacaagacg gtgccgatg atgaatctg atgtgccaa tggctcaaa ggaagcgtt tcacgagag tggaaatgg cacgtgttc tcatggagc gcaaccaat acaaagagc gcttcatgg agttgcaag aagccgtcc gttggacgg agatgattc gagcgtccc gtaacgaca ccgcctttg acaaaaaga gcaaacaga gcatttgga ggttcttta gtaatttgt ttagtggtt cggagcggc cgcaaagac cattggttg gtccacatc tacgttacc agacggcga ccaatcaaa tagcgccag gtgttagag ccctgcctc gacccggtc atgactttg tcgaaactg aattgggta cggaagtgt gattatttt cttactatt tgtacctgc tgtcaatct gtgatatgt tactactag agtagatct gagcaaaaa tcgaaattt gggttggta gacctgtct gcttttggt ggtgcgttg gagtgcatg atttttttt taacccccg actgtaatg aaacacgga atgttcaat tatttttta tccatttat taatccaaa gttacggta actaatcta gtcttactc aatttgtta ccacaataa ttgttacgt ttgttacaa atacatttt aataatttc c
- >TC003186 RD
gcagtggca aaaatgaca gttgttcct ccagcccga tttttgtca tcataaaca gtgggtgta acaaaaaat cgcacactt ttccatcac cagtgcccc cagaatgtg ccccacgtt gtaaaatca tcacaacac aatgttccc tcaagtgac aatgtgcta ttcttgatt gggcaaaag taggttgaa tagttggtt aaataatgg atttggctg actccggcg gtggtcttg gacccgaga ccatctacg ggacgcacg aggactccc atgtggtga tgtcggaga aggtgcaat cgttggctg gtagcatct accaggagt tcgaacgaa tgatcgcac gctacgacg aagacgtcg tcaagaccc tcatgcccc tgctggtga acgtgctgg agtgtttgg acgcggcgt atcaacaga accaggagc acgacgtgg agctggagc tgctccgcg aagacaacg agcagttgg tcacgcagt acgagcgcg agaaggggg cgcgcaaag ccgccgagc agaaactcc tagagtttg aagacgccg ccgaagggg agcgcaagg agttggccg cacggctcg aagcgctgg aaagcatcg tcaggatgc tggagctga agcacaaga actcgatgg agcatgcgg gacgtttgg aggagcgtg aaggggagt tgaagaagg aatacgcca agttgcacg aacgctaca ccgagttgt tcaaaacac acatggact acatggagc ggacgaagt tgctgcttt ctggacaac agctggcca atgagcggg ccgaagccg gccgtttga actcgaatc ggattaata tagggcgca gttcggggc ccatctcct tcgggttca cctccttgg agaatgccg aggtggtgg acagtgttc cgtcgtcgc cgattagta gtacgcatt cgtcgccga gtttgcaca gcgagttgg ataatttgg acaagaaga tggtggaaa gggggcaag agactgatt cgctcaatt tggaaaata aaagtgtga taacatcgc cggtgagtc ccccaggtc aaccgacaa ctaacaata atacgggga ggttgaaga aggaacaaa ggagtggga acacgttgt atcaagagt tgagttttc aggatgtgg atggcgatg atgatggtg acataaccg gaggttggg tccatccag gtgaatacg cttcatcag tcaacgata atttttacg gaatgggca aagaagtcg aaaatttga taatggaaa acaacgagc tactagcca ccaaaaacg cccttaatg tggtaaaag acgacctta tcgtaaaag ttgacgaat taaccggcg aaattgaaa tgcttcgcg aagaaatcc tctcgttaa atgtctcac gaacgaaac tccgtgaaa gaatcagtg atctcgaag aagaattgc gaaaagtta aggaagcaa atgaagcga aacaagacg gtgccgatg atgaatctg atgtgccaa tggctcaaa ggaagcgtt tcacgagag tggaaatgg cacgtgttc tcatggagc gcaaccaat acaaagagc gcttcatgg agttgcaag aagccgtcc gttggacgg agatgattc gagcgtccc gtaacgaca ccgcctttg acaaaaaga gcaaacaga gcatttgga ggttcttta gtaatttgt ttagtggtt cggagcggc cgcaaagac cattggttg gtccacatc tacgttacc agacggcga ccaatcaaa tagcgccag gtgttagag ccctgcctc gacccggtc atgactttg tcgaaactg aattgggtt cggaaaagt tggctttga ggagggctg ttgagagac gagaacagt accgacaag tgagagcgc atgtgcgaa aagaggatg gaagattgc aagcgtatg gttggagtt tgcccggaa aagttggtc cgggaacga aaggaacac aaagtagtt ccggggttc cggtcccgg tcccggttt attgtaggc cattggctg aaactacgc ccgggatga agatttggt gtgcagcgg gtgttaatc tcatggggg gttacacca aagatgggg ggcttatgg tggggggca gtgtcttct actcggaag agccccgcc tggagcagc cccacaccg agctagaga ctctggatc aggagctga atgcaggtc gtgaagggg cacttctcg agacaaaat tatcgtcac ttgtgtgga tttgtacat acacacatg ctgctagtt tagtaaccg taatcgatg cgaataatc cagcggaga ttttgaata gttttgggg tttgtgcaa accatgttt tatgcattg ctagtgtac ctggtgcta gtccaaacg attataata aaacgtata ccaaaatta acgttagtg agaatgcaa aagacagca gtgataacg tcgtagaaa ataaagaag acgaagggg aaaataata caaacaaaa cgaatgata aaaatcacg aagagttgg aagaggcgg ctttgattg ggaaagtta cgtttgttg aaaacgata cttctgtgg ttaataaaa aagacaaat ataataatg caaaatcaa cggtggatg aagagaaaa ttgaggaac aacctgttg aggatgaaa ctgatgaaa atgtgggtg aaagtgata agaataaaa cgagcaatc aggaaataa tgtcgagtg ttttggcaa caatgtggc taggagcgg aaaatggaa tggtttatg ttcactcat cggtggcca attggaatc agtgtctgc attccgtca aacttaaag atgcagtga taagtattg tacatataa atggccgtg tcgtttgtg ctttggctt gtggtactt tggcaattt tcgcacgaa acgatgatg gagaatggg acttgtccc gttaccatt tggtacaag tgggtttac cacatcagt ccgttcgtt gtttggctg ttgttggag ataaagtct ggtgcggat ataggaata aaattcacg tgcttgaac cacaagaat tgggaataa ttcattcgc tggaagcgc atcctcgac gtgaaagtc ccgtgcgac aaattgcgt ggttgggtg aaggtgttt gggtttcga ttcgtttag attcgactt taagactgt accatgctc atacctacc agcatttgc aggatgtgg atattgagc cgtacgtca gcaagatgt tgggtactg gaaagctgg gcttttcct tcgtgcgca taacgtgtc ttttgatat cgtcaaacc gcctctgga ttggcacaa gcaacggtg tcataattt ccgttccat tgagcgaga acggggcaa acaccggaa gcatagttc cccggggat caaccagtc ttcccggag cgggagtac gtgttgctc ccgttcccg gaagcttta tcccttttt gtaccatgg cccacgctc aactcagct tccacggcc atagagata acgtgaaat tttttgtcg ccgtccccg gaaacggag gaatgagcg cggcttcga cgccttcag aggcactgt cgacttctg cgacttcga tcgggttgc cccccaagc agccgtcgg caatgctgg tgatgtcgg ggggcgaag gttacatcg atttccgcg tcgaggacg aaaatgaac cgagagatg gagactcgc atctgctcg tttggcagg ttttgcctg atattcctg ggaatcctg tggggtgat gagatggag gatagcgtt attgatact gaaaccggt gatcttgaa gcagctcag ggcgaatcc aaaggggaa aaaagtcac ttgattgtg tggcaagtg tccacaagt taattaagc aaaatgata tttaacatt aattgaaat actaaacat tagcggttt tagattagt aacgtttat aaaaaaaac aacttggac gatatttta gcttgtgaa tatgttatt gatacataa tattttatc gttgtttta tatctgtta catgtaaaa actatttat tatgatttg taaatttga gacgtgtat ttaggaaaa tttttattt aaatgtatc gtgcttaca ctgtaaatg aaacttata caaactgaa tgcttttta ttcaaataa gacccactc acaaatgcg attgcattg acgtttgag gttatgtta gtgttcttt tgatgccat atgtcgcat taaattgtt gataaattt gtctatttt tcgcacatc ttgggcaat ctccgtttt gaattgtaa acac
- >TC003186 RE
aaaaaatta tttgaaaaa aattgtagg ttcggaaaa gttggcttt gaggagggc tgttgagag acgagaaca gtaccgaca agtgagagc gcatgtgcg aaaagagga tggaagatt gcaagcgta tggttggag tttgcccgg aaaagttgg tccgggaac gaaaggaac acaaagtag ttccggggt tccggtccc ggtcccggt ttattgtag gccattggc tgaaactac gcccgggat gaagatttg gtgtgcagc gggtgttaa tctcatggg gggttacac caaagatgg ggggcttat ggtgggggg cagtgtctt ctactcgga agagccccg cctggagca gccccacac cgagctaga gactctgga tcaggagct gaatgcagg tcgtgaagg ggcacttct cgagacaaa attatcgtc acttgtgtg gatttgtac atacacaca tgctgctag tttagtaac cgtaatcga tgcgaataa tccagcgga gattttgaa tagttttgg ggtttgtgc aaaccatgt tttatgcat tgctagtgt acctggtgc tagtccaaa cgattataa taaaacgta taccaaaat taacgttag tgagaatgc aaaagacag cagtgataa cgtcgtaga aaataaaga agacgaagg ggaaaataa tacaaacaa aacgaatga taaaaatca cgaagagtt ggaagaggc ggctttgat tgggaaagt tacgtttgt tgaaaacga tacttctgt ggttaataa aaaagacaa atataataa tgcaaaatc aacggtgga tgaagagaa aattgagga acaacctgt tgaggatga aactgatga aaatgtggg tgaaagtga taagaataa aacgagcaa tcaggaaat aatgtcgag tgttttggc aacaatgtg gctaggagc ggaaaatgg aatggttta tgttcactc atcggtggc caattggaa tcagtgtct gcattccgt caaacttaa agatgcagt gataagtat tgtacatat aaatggccg tgtcgtttg tgctttggc ttgtggtac tttggcaat tttcgcacg aaacgatga tggagaatg ggacttgtc ccgttacca tttggtaca agtgggttt accacatca gtccgttcg ttgtttggc tgttgttgg agataaagt ctggtgcgg atataggaa taaaattca cgtgcttga accacaaga attgggaat aattcattc gctggaagc gcatcctcg acgtgaaag tcccgtgcg acaaattgc gtggttggg tgaaggtgt ttgggtttc gattcgttt agattcgac tttaagact gtaccatgc tcataccta ccagcattt gcaggatgt ggatattga gccgtacgt cagcaagat gttgggtac tggaaagct gggcttttc cttcgtgcg cataacgtg tcttttgat atcgtcaaa ccgcctctg gattggcac aagcaacgg tgtcataat ttccgttcc attgagcga gaacggggc aaacaccgg aagcatagt tccccgggg atcaaccag tcttcccgg agcgggagt acgtgttgc tcccgttcc cggaagctt tatcccttt ttgtaccat ggcccacgc tcaactcag cttccacgg ccatagaga taacgtgaa attttttgt cgccgtccc cggaaacgg aggaatgag cgcggcttc gacgccttc agaggcact gtcgacttc tgcgacttc gatcgggtt gccccccaa gcagccgtc ggcaatgct ggtgatgtc ggggggcga aggttacat cgatttccg cgtcgagga cgaaaatga accgagaga tggagactc gcatctgct cgtttggca ggttttgcc tgatattcc tgggaatcc tgtggggtg atgagatgg aggatagcg ttattgata ctgaaaccg gtgatcttg aagcagctc agggcgaat ccaaagggg aaaaaagtc acttgattg tgtggcaag tgtccacaa gttaattaa gcaaaatga tatttaaca ttaattgaa atactaaac attagcggt tttagatta gtaacgttt ataaaaaaa acaacttgg acgatattt tagcttgtg aatatgtta ttgatacat aatatttta tcgttgttt tatatctgt tacatgtaa aaactattt attatgatt tgtaaattt gagacgtgt atttaggaa aatttttat ttaaatgta tcgtgctta cactgtaaa tgaaactta tacaaactg aatgctttt tattcaaat aagacccac tcacaaatg cgattgcat tgacgtttg aggttatgt tagtgttct tttgatgcc atatgtcgc attaaattg ttgataaat ttgtctatt tttcgcaca tcttgggca atctccgtt ttgaattgt aaacac
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