Status
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Gene information
General information
TC032287
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003237,TC003236
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 163499..168330
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032287
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02681

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    egg chamber number decreased - Number of animals showing the phenotype: 7

    vitellogenic egg chamber latenot present - Number of animals showing the phenotype: 7

    ovary size decreased - Number of animals showing the phenotype: 7
    Analysis of larval stage 1 cuticle
    remarks - cuticles: bad quality of preparation,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection



    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    Pupal morphology
    head orientation irregular - Number of animals showing the phenotype: 5

    antenna shortened - Number of animals showing the phenotype: 5
    eyes slightly shape irregular - Number of animals showing the phenotype: 5

    Comment: dorsal part seems to be smaller
    wings shortened - Number of animals showing the phenotype: 5

    legs sometimes shortened - Number of animals showing the phenotype: 2

    legs sometimes shape irregular - Number of animals showing the phenotype: 2
    female genital lobes shortened - Number of animals showing the phenotype: 5
    female genital lobes orientation irregular - Number of animals showing the phenotype: 3

    wings size decreased - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection



    legs shortened - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032287 RA
      MTRGRGYSP RGYRGGSGR GSWGGGRGG YHSRGGGRN 
      FDSSFSDSF DSRSKYGGS SDRYSGSRG HGHGHDDFR 
      KSYRMDSGY SGRDSGHRS PDRKRPRNE APSSRHDSS 
      YGGYGSSTS RYDSSYSDR RSFSSDRHP GSFSRRDDF 
      RRPSGPPRG GYRGRISSR GSRGLKLRD RPTRRRLVE 
      SSYAVRKRI IPSRTSDYA RKLKISKMR SAIAARKKR 
      ASDKEDSDE EKEEKDEKN EEEEEEDKN ENNEGVEED 
      KKPTNKEEI SDKEDKGEK GDEPAMRKT FIKLNCPHC 
      GIKAVTFRK YDLHLQSRT HMMAMRKVA IKQKSILAQ 
      MRQAQRNAQ NELEKTSDD LAPRTNFCP LCKLNYKQR 
      KAVHQNSDA HKNMKKFLM PYCKLCSIT FKSPMMYES 
      HCCSIEHLK RKQRVENGE NSDEFDDEA NLENFTTID 
      SVGNDGDVS EEDDKKDTN KEPVNVGIE QIRKVEAYY 
      CDLCKMYVG RGSDDMITP ILARHCKQR THMQRYIRY 
      KEDKDLEKR AEKLQRKET AEKEGKEKQ VKENTEETT 
      PKNKKEEQE TEKDDESAS KVLDDEEAR DDKLWADVD 
      KDLDDILAK EGTKSSDED EDVNGERYD RFKYSEKNG 
      EEKVVKEGE GGEAVAAEV KVAEVTAEK
    • >TC032287 RB
      MRGVFSNSK YSYSQTAAM TRGRGYSPR GYRGGSGRG 
      SWGGGRGGY HSRGGGRNF DSSFSDSFD SRSKYGGSS 
      DRYSGSRGH GHGHDDFRK SYRMDSGYS GRDSGHRSP 
      DRKRPRNEA PSSRHDSSY GGYGSSTSR YDSSYSDRR 
      SFSSDRHPG SFSRRDDFR RPSGPPRGG YRGRISSRG 
      SRGLKLRDR PTRRRLVES SYAVRKRII PSRTSDYAR 
      KLKISKMRS AIAARKKRA SDKEDSDEE KEEKDEKNE 
      EEEEEDKNE NNEGVEEDK KPTNKEEIS DKEDKGEKG 
      DEPAMRKTF IKLNCPHCG IKAVTFRKY DLHLQSRTH 
      MMAMRKVAI KQKSILAQM RQAQRNAQN ELEKTSDDL 
      APRTNFCPL CKLNYKQRK AVHQNSDAH KNMKKFLMP 
      YCKLCSITF KSPMMYESH CCSIEHLKR KQRVENGEN 
      SDEFDDEAN LENFTTIDS VGNDGDVSE EDDKKDTNK 
      EPVNVGIEQ IRKVEAYYC DLCKMYVGR GSDDMITPI 
      LARHCKQRT HMQRYIRYK EDKDLEKRA EKLQRKETA 
      EKEGKEKQV KENTEETTP KNKKEEQET EKDDESASK 
      VLDDEEARD DKLWADVDK DLDDILAKE GTKSSDEDE 
      DVNGERYDR FKYSEKNGE EKVVKEGEG GEAVAAEVK 
      VAEVTAEK
    CDS sequence
    • >TC032287 RA
      atgacacgt ggtcggggg tattctcca cgtgggtat 
      cgtgggggt tcgggacgt ggttcatgg ggcggcgga 
      cgaggcggt tatcatagc cgaggcggt ggcaggaac 
      ttcgattca tcgttttca gactctttt gactcgagg 
      tccaaatat gggggctcc agcgatcgg tattcgggc 
      tccagggga catggccac ggccatgat gatttccgc 
      aagtcgtat cgcatggat tcgggctat tctgggcgt 
      gatagtggt catcgttct cccgaccgc aaaagaccg 
      cgaaacgag gcccctagt tcacgtcac gatagttct 
      tatggtggt tatggatcg agcacaagt cgttatgat 
      tcaagttat tcagatcga agaagtttt tcgagtgac 
      cgacatcct ggatcgttt tcacgaaga gatgacttt 
      cgtagaccg tctggtcct ccaagagga ggatacaga 
      ggacgtatc agttctagg ggctcacgg ggcctgaag 
      cttagagat cgtccaaca cgaagacgc ctcgttgaa 
      agttcatat gccgtgcgt aaacgaatt ataccttca 
      cgtacatca gactacgcc aggaaactt aaaatttcc 
      aaaatgcgg agcgcaata gcagctcgt aagaaacga 
      gcaagtgac aaggaagat tccgacgag gaaaaggaa 
      gaaaaagac gagaagaac gaagaagag gaggaagaa 
      gataagaac gaaaacaat gaaggagtc gaagaagat 
      aagaaaccc acaaataaa gaagaaatt tccgataaa 
      gaagacaag ggtgaaaag ggagatgaa cccgcaatg 
      cgcaaaact ttcatcaaa ttgaactgt ccccattgt 
      ggaattaaa gccgtcact ttcagaaag tacgatttg 
      catttgcaa agtcgtact catatgatg gcgatgcgt 
      aaagttgca attaagcaa aaatcaatt cttgctcag 
      atgagacag gcgcagaga aatgctcag aacgagttg 
      gagaaaaca agcgatgat ttagcacca aggactaat 
      ttctgtccg ttgtgcaaa cttaattac aaacaacgt 
      aaagctgtt catcaaaat tccgatgcg cataaaaat 
      atgaaaaag tttttgatg ccgtactgc aaattgtgt 
      agtatcaca tttaagagt cctatgatg tatgagagt 
      cattgctgt tctattgaa catttgaag agaaaacaa 
      cgcgtcgaa aatggagag aacagtgat gaatttgat 
      gatgaagcc aaccttgaa aactttaca actattgat 
      tcagttggt aatgatggt gatgtgtct gaagaagat 
      gataaaaaa gacacaaat aaagaacct gtaaatgtt 
      gggattgaa cagattcgt aaagtcgag gcatattat 
      tgtgatttg tgtaaaatg tacgttggt cgtggtagc 
      gatgatatg ataacccca attttggca agacattgc 
      aagcaacga actcacatg caaagatac atccgatac 
      aaggaagat aaagatctt gaaaaacgc gcagagaaa 
      ttacaacga aaagaaacc gctgaaaaa gagggtaaa 
      gagaaacaa gttaaggaa aatacagaa gaaaccaca 
      ccgaagaat aagaaagag gaacaagag actgaaaaa 
      gatgatgaa tcggctagc aaagtcctc gacgatgaa 
      gaagccaga gacgataaa ctttgggcc gatgttgac 
      aaagattta gacgatatt ttggctaaa gaaggaacg 
      aaaagttct gatgaagat gaagacgta aacggggaa 
      cgttatgat agatttaaa tatagcgaa aagaacggt 
      gaggaaaaa gtcgtcaaa gaaggtgaa gggggcgaa 
      gccgttgcg gccgaagtg aaagttgct gaagttaca 
      gctgaaaag taa
    • >TC032287 RB
      atgcgtggc gttttttcg aattcgaag tactcttac 
      agtcaaaca gcggccatg acacgtggt cgggggtat 
      tctccacgt gggtatcgt gggggttcg ggacgtggt 
      tcatggggc ggcggacga ggcggttat catagccga 
      ggcggtggc aggaacttc gattcatcg ttttcagac 
      tcttttgac tcgaggtcc aaatatggg ggctccagc 
      gatcggtat tcgggctcc aggggacat ggccacggc 
      catgatgat ttccgcaag tcgtatcgc atggattcg 
      ggctattct gggcgtgat agtggtcat cgttctccc 
      gaccgcaaa agaccgcga aacgaggcc cctagttca 
      cgtcacgat agttcttat ggtggttat ggatcgagc 
      acaagtcgt tatgattca agttattca gatcgaaga 
      agtttttcg agtgaccga catcctgga tcgttttca 
      cgaagagat gactttcgt agaccgtct ggtcctcca 
      agaggagga tacagagga cgtatcagt tctaggggc 
      tcacggggc ctgaagctt agagatcgt ccaacacga 
      agacgcctc gttgaaagt tcatatgcc gtgcgtaaa 
      cgaattata ccttcacgt acatcagac tacgccagg 
      aaacttaaa atttccaaa atgcggagc gcaatagca 
      gctcgtaag aaacgagca agtgacaag gaagattcc 
      gacgaggaa aaggaagaa aaagacgag aagaacgaa 
      gaagaggag gaagaagat aagaacgaa aacaatgaa 
      ggagtcgaa gaagataag aaacccaca aataaagaa 
      gaaatttcc gataaagaa gacaagggt gaaaaggga 
      gatgaaccc gcaatgcgc aaaactttc atcaaattg 
      aactgtccc cattgtgga attaaagcc gtcactttc 
      agaaagtac gatttgcat ttgcaaagt cgtactcat 
      atgatggcg atgcgtaaa gttgcaatt aagcaaaaa 
      tcaattctt gctcagatg agacaggcg cagagaaat 
      gctcagaac gagttggag aaaacaagc gatgattta 
      gcaccaagg actaatttc tgtccgttg tgcaaactt 
      aattacaaa caacgtaaa gctgttcat caaaattcc 
      gatgcgcat aaaaatatg aaaaagttt ttgatgccg 
      tactgcaaa ttgtgtagt atcacattt aagagtcct 
      atgatgtat gagagtcat tgctgttct attgaacat 
      ttgaagaga aaacaacgc gtcgaaaat ggagagaac 
      agtgatgaa tttgatgat gaagccaac cttgaaaac 
      tttacaact attgattca gttggtaat gatggtgat 
      gtgtctgaa gaagatgat aaaaaagac acaaataaa 
      gaacctgta aatgttggg attgaacag attcgtaaa 
      gtcgaggca tattattgt gatttgtgt aaaatgtac 
      gttggtcgt ggtagcgat gatatgata accccaatt 
      ttggcaaga cattgcaag caacgaact cacatgcaa 
      agatacatc cgatacaag gaagataaa gatcttgaa 
      aaacgcgca gagaaatta caacgaaaa gaaaccgct 
      gaaaaagag ggtaaagag aaacaagtt aaggaaaat 
      acagaagaa accacaccg aagaataag aaagaggaa 
      caagagact gaaaaagat gatgaatcg gctagcaaa 
      gtcctcgac gatgaagaa gccagagac gataaactt 
      tgggccgat gttgacaaa gatttagac gatattttg 
      gctaaagaa ggaacgaaa agttctgat gaagatgaa 
      gacgtaaac ggggaacgt tatgataga tttaaatat 
      agcgaaaag aacggtgag gaaaaagtc gtcaaagaa 
      ggtgaaggg ggcgaagcc gttgcggcc gaagtgaaa 
      gttgctgaa gttacagct gaaaagtaa
    mRNA sequence
    • >TC032287 RA
      tgtcagacg ccattgtcg taagcgtcc tagccggta 
      tttgttgct ggtttttat tttcataaa aaattatct 
      ttatttttc acctacaca cataaaaac agtgttatc 
      acacgtccc gttgtgtaa tttcgccaa taccctggt 
      tcgttacag tcaaacagc ggccatgac acgtggtcg 
      ggggtattc tccacgtgg gtatcgtgg gggttcggg 
      acgtggttc atggggcgg cggacgagg cggttatca 
      tagccgagg cggtggcag gaacttcga ttcatcgtt 
      ttcagactc ttttgactc gaggtccaa atatggggg 
      ctccagcga tcggtattc gggctccag gggacatgg 
      ccacggcca tgatgattt ccgcaagtc gtatcgcat 
      ggattcggg ctattctgg gcgtgatag tggtcatcg 
      ttctcccga ccgcaaaag accgcgaaa cgaggcccc 
      tagttcacg tcacgatag ttcttatgg tggttatgg 
      atcgagcac aagtcgtta tgattcaag ttattcaga 
      tcgaagaag tttttcgag tgaccgaca tcctggatc 
      gttttcacg aagagatga ctttcgtag accgtctgg 
      tcctccaag aggaggata cagaggacg tatcagttc 
      taggggctc acggggcct gaagcttag agatcgtcc 
      aacacgaag acgcctcgt tgaaagttc atatgccgt 
      gcgtaaacg aattatacc ttcacgtac atcagacta 
      cgccaggaa acttaaaat ttccaaaat gcggagcgc 
      aatagcagc tcgtaagaa acgagcaag tgacaagga 
      agattccga cgaggaaaa ggaagaaaa agacgagaa 
      gaacgaaga agaggagga agaagataa gaacgaaaa 
      caatgaagg agtcgaaga agataagaa acccacaaa 
      taaagaaga aatttccga taaagaaga caagggtga 
      aaagggaga tgaacccgc aatgcgcaa aactttcat 
      caaattgaa ctgtcccca ttgtggaat taaagccgt 
      cactttcag aaagtacga tttgcattt gcaaagtcg 
      tactcatat gatggcgat gcgtaaagt tgcaattaa 
      gcaaaaatc aattcttgc tcagatgag acaggcgca 
      gagaaatgc tcagaacga gttggagaa aacaagcga 
      tgatttagc accaaggac taatttctg tccgttgtg 
      caaacttaa ttacaaaca acgtaaagc tgttcatca 
      aaattccga tgcgcataa aaatatgaa aaagttttt 
      gatgccgta ctgcaaatt gtgtagtat cacatttaa 
      gagtcctat gatgtatga gagtcattg ctgttctat 
      tgaacattt gaagagaaa acaacgcgt cgaaaatgg 
      agagaacag tgatgaatt tgatgatga agccaacct 
      tgaaaactt tacaactat tgattcagt tggtaatga 
      tggtgatgt gtctgaaga agatgataa aaaagacac 
      aaataaaga acctgtaaa tgttgggat tgaacagat 
      tcgtaaagt cgaggcata ttattgtga tttgtgtaa 
      aatgtacgt tggtcgtgg tagcgatga tatgataac 
      cccaatttt ggcaagaca ttgcaagca acgaactca 
      catgcaaag atacatccg atacaagga agataaaga 
      tcttgaaaa acgcgcaga gaaattaca acgaaaaga 
      aaccgctga aaaagaggg taaagagaa acaagttaa 
      ggaaaatac agaagaaac cacaccgaa gaataagaa 
      agaggaaca agagactga aaaagatga tgaatcggc 
      tagcaaagt cctcgacga tgaagaagc cagagacga 
      taaactttg ggccgatgt tgacaaaga tttagacga 
      tattttggc taaagaagg aacgaaaag ttctgatga 
      agatgaaga cgtaaacgg ggaacgtta tgatagatt 
      taaatatag cgaaaagaa cggtgagga aaaagtcgt 
      caaagaagg tgaaggggg cgaagccgt tgcggccga 
      agtgaaagt tgctgaagt tacagctga aaagtaatt 
      aattttaat atggaatga ctgttaact tttacgtgg 
      aaatacctg tgtttgaca agtgtagca agttttgtt 
      taacagttt tattttaat tataattag ggatattta 
      cagtttcgt aggtttaga aatcaattt gaagacttt 
      gctatcaag atgtgaagc atagtgata ttcattcta 
      catgccatc aaataaaaa ataaaaaaa att
    • >TC032287 RB
      tgtcagacg ccattgtcg taagcgtcc tagccggta 
      tttgttgct ggtttttat tttcataaa aaattatct 
      ttatttttc acctacaca cataaaaac agtgttatc 
      acacgtccc gttgtgtaa tttcgccaa taccctggt 
      tcgtaaacc atgagttat tacaagccc aggtaagaa 
      tgcgtggcg ttttttcga attcgaagt actcttaca 
      gtcaaacag cggccatga cacgtggtc gggggtatt 
      ctccacgtg ggtatcgtg ggggttcgg gacgtggtt 
      catggggcg gcggacgag gcggttatc atagccgag 
      gcggtggca ggaacttcg attcatcgt tttcagact 
      cttttgact cgaggtcca aatatgggg gctccagcg 
      atcggtatt cgggctcca ggggacatg gccacggcc 
      atgatgatt tccgcaagt cgtatcgca tggattcgg 
      gctattctg ggcgtgata gtggtcatc gttctcccg 
      accgcaaaa gaccgcgaa acgaggccc ctagttcac 
      gtcacgata gttcttatg gtggttatg gatcgagca 
      caagtcgtt atgattcaa gttattcag atcgaagaa 
      gtttttcga gtgaccgac atcctggat cgttttcac 
      gaagagatg actttcgta gaccgtctg gtcctccaa 
      gaggaggat acagaggac gtatcagtt ctaggggct 
      cacggggcc tgaagctta gagatcgtc caacacgaa 
      gacgcctcg ttgaaagtt catatgccg tgcgtaaac 
      gaattatac cttcacgta catcagact acgccagga 
      aacttaaaa tttccaaaa tgcggagcg caatagcag 
      ctcgtaaga aacgagcaa gtgacaagg aagattccg 
      acgaggaaa aggaagaaa aagacgaga agaacgaag 
      aagaggagg aagaagata agaacgaaa acaatgaag 
      gagtcgaag aagataaga aacccacaa ataaagaag 
      aaatttccg ataaagaag acaagggtg aaaagggag 
      atgaacccg caatgcgca aaactttca tcaaattga 
      actgtcccc attgtggaa ttaaagccg tcactttca 
      gaaagtacg atttgcatt tgcaaagtc gtactcata 
      tgatggcga tgcgtaaag ttgcaatta agcaaaaat 
      caattcttg ctcagatga gacaggcgc agagaaatg 
      ctcagaacg agttggaga aaacaagcg atgatttag 
      caccaagga ctaatttct gtccgttgt gcaaactta 
      attacaaac aacgtaaag ctgttcatc aaaattccg 
      atgcgcata aaaatatga aaaagtttt tgatgccgt 
      actgcaaat tgtgtagta tcacattta agagtccta 
      tgatgtatg agagtcatt gctgttcta ttgaacatt 
      tgaagagaa aacaacgcg tcgaaaatg gagagaaca 
      gtgatgaat ttgatgatg aagccaacc ttgaaaact 
      ttacaacta ttgattcag ttggtaatg atggtgatg 
      tgtctgaag aagatgata aaaaagaca caaataaag 
      aacctgtaa atgttggga ttgaacaga ttcgtaaag 
      tcgaggcat attattgtg atttgtgta aaatgtacg 
      ttggtcgtg gtagcgatg atatgataa ccccaattt 
      tggcaagac attgcaagc aacgaactc acatgcaaa 
      gatacatcc gatacaagg aagataaag atcttgaaa 
      aacgcgcag agaaattac aacgaaaag aaaccgctg 
      aaaaagagg gtaaagaga aacaagtta aggaaaata 
      cagaagaaa ccacaccga agaataaga aagaggaac 
      aagagactg aaaaagatg atgaatcgg ctagcaaag 
      tcctcgacg atgaagaag ccagagacg ataaacttt 
      gggccgatg ttgacaaag atttagacg atattttgg 
      ctaaagaag gaacgaaaa gttctgatg aagatgaag 
      acgtaaacg gggaacgtt atgatagat ttaaatata 
      gcgaaaaga acggtgagg aaaaagtcg tcaaagaag 
      gtgaagggg gcgaagccg ttgcggccg aagtgaaag 
      ttgctgaag ttacagctg aaaagtaat taattttaa 
      tatggaatg actgttaac ttttacgtg gaaatacct 
      gtgtttgac aagtgtagc aagttttgt ttaacagtt 
      ttattttaa ttataatta gggatattt acagtttcg 
      taggtttag aaatcaatt tgaagactt tgctatcaa 
      gatgtgaag catagtgat attcattct acatgccat 
      caaataaaa aataaaaaa aatt
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