Status
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Gene information
General information
TC032014 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 4119083..4122630
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032014
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02680

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032014 RA
      MSRRKKIKV DSKEIDDKL NNADEENVA EASDKTKTL 
      PSEKKLNSG ASSSKGNKK ESKKEELKK EEDKKKDID 
      PESELDDPV IKDLFSGLE GAAFQSRMP FDKMTSTEA 
      ACFPDIAQA ALQTIKVYL NVRNRILQM WLDNPKQQL 
      ILEDVMEKI EVPYNSDAP LVKRVHAFL ERNGYINFG 
      VFKRLKPLP SKKYGKVIV IGAGIAGLA AAQQLQQFG 
      LEVIVLEAR DRVGGRIAT FRKGNYIAD LGAMVVTGL 
      GGNPVTTLS KQIDMELHR IRQKCPLYQ SSGVTVDKD 
      KDEMVEREF NRLLEATSY LSHQLDFNY AGNKPVSLG 
      QALEWVIKL QEKHVKEKQ IQHLKSVIT LQEKLKTNQ 
      KLLVGIKEQ MFETNAKIR ELASVEKRD IQLEFAYRS 
      TVRDLNSYS KEWDQLQEQ AKEIEDKLK ELEASPPSD 
      VYLSSKDRQ ILDWHFANL EFANATPLS NLSLKHWDQ 
      DDDFEFTGN HLTGEWAQL
    CDS sequence
    • >TC032014 RA
      atgagtcgg agaaaaaag ataaaggtg gacagtaaa 
      gaaattgat gataaactt aacaacgcc gatgaggaa 
      aatgttgcg gaagcgtcg gataaaact aaaaccctt 
      ccatctgag aaaaaatta aactcaggg gctagttct 
      tccaaggga aacaagaag gaatcaaag aaggaagag 
      ttgaagaag gaggaggat aagaagaag gatattgat 
      cccgaatcg gagctggat gatccagta atcaaagac 
      ttattttct gggttagaa ggagcagca tttcagagt 
      cgcatgcca tttgataaa atgacctca actgaagcg 
      gcctgtttc ccagatata gcccaagcc gcattacag 
      accattaaa gtgtaccta aacgtccgc aatcgtatt 
      ctacaaatg tggctcgat aacccaaaa caacagcta 
      atacttgaa gacgttatg gaaaaaatc gaagttccc 
      tacaacagt gacgcgcct ctcgttaaa agagttcac 
      gcgtttttg gaacgtaac gggtacatc aattttggt 
      gttttcaaa agacttaaa cctttgccg tcgaaaaaa 
      tatggaaag gttattgta attggagcc ggcattgct 
      ggactagct gcagctcaa caattacaa caatttgga 
      ctggaagta attgtccta gaagcgcgg gatagagta 
      ggtggaaga attgccact tttagaaaa ggtaattat 
      attgctgac ttgggggct atggttgtg acaggcctc 
      ggtggcaac cctgtgaca accctcagc aaacaaatc 
      gacatggaa ctgcaccgc atccgacag aaatgtcct 
      ctgtaccaa tcaagcggt gttactgtc gataaagac 
      aaagatgaa atggttgaa cgtgaattt aaccgactt 
      ttggaagcc acatcgtac ttatcgcat caactagat 
      tttaattat gctggaaat aagccagtt agtttaggt 
      caagcgtta gaatgggtg ataaagctt caagaaaag 
      cacgttaaa gagaaacaa atccagcat ctcaaatcc 
      gttataaca ctacaagaa aaattgaaa acgaatcaa 
      aaactctta gttgggatt aaggaacag atgtttgag 
      accaatgca aaaattagg gaactggcc agtgttgaa 
      aaacgagat attcagcta gaatttgcg tataggagt 
      actgtgcgt gatcttaac tcatattcg aaagagtgg 
      gatcaacta caagagcaa gcaaaggag attgaagat 
      aaacttaaa gaattagaa gcttcgcca ccaagtgat 
      gtgtacttg tcatcgaaa gatcgccag attttggac 
      tggcatttt gccaacttg gaatttgca aatgcgact 
      ccactttcg aatctatcg ctgaaacat tgggatcag 
      gacgacgat tttgaattc acggggaat cacttaaca 
      ggtgagtgg gcgcaactg taa
    mRNA sequence
    • >TC032014 RA
      tgtcaagga cgtaaacaa aaatcaaaa taatcagtt 
      cattttcca cttttttga taattttac cagataaaa 
      cgttaaaaa atgagtcgg agaaaaaag ataaaggtg 
      gacagtaaa gaaattgat gataaactt aacaacgcc 
      gatgaggaa aatgttgcg gaagcgtcg gataaaact 
      aaaaccctt ccatctgag aaaaaatta aactcaggg 
      gctagttct tccaaggga aacaagaag gaatcaaag 
      aaggaagag ttgaagaag gaggaggat aagaagaag 
      gatattgat cccgaatcg gagctggat gatccagta 
      atcaaagac ttattttct gggttagaa ggagcagca 
      tttcagagt cgcatgcca tttgataaa atgacctca 
      actgaagcg gcctgtttc ccagatata gcccaagcc 
      gcattacag accattaaa gtgtaccta aacgtccgc 
      aatcgtatt ctacaaatg tggctcgat aacccaaaa 
      caacagcta atacttgaa gacgttatg gaaaaaatc 
      gaagttccc tacaacagt gacgcgcct ctcgttaaa 
      agagttcac gcgtttttg gaacgtaac gggtacatc 
      aattttggt gttttcaaa agacttaaa cctttgccg 
      tcgaaaaaa tatggaaag gttattgta attggagcc 
      ggcattgct ggactagct gcagctcaa caattacaa 
      caatttgga ctggaagta attgtccta gaagcgcgg 
      gatagagta ggtggaaga attgccact tttagaaaa 
      ggtaattat attgctgac ttgggggct atggttgtg 
      acaggcctc ggtggcaac cctgtgaca accctcagc 
      aaacaaatc gacatggaa ctgcaccgc atccgacag 
      aaatgtcct ctgtaccaa tcaagcggt gttactgtc 
      gataaagac aaagatgaa atggttgaa cgtgaattt 
      aaccgactt ttggaagcc acatcgtac ttatcgcat 
      caactagat tttaattat gctggaaat aagccagtt 
      agtttaggt caagcgtta gaatgggtg ataaagctt 
      caagaaaag cacgttaaa gagaaacaa atccagcat 
      ctcaaatcc gttataaca ctacaagaa aaattgaaa 
      acgaatcaa aaactctta gttgggatt aaggaacag 
      atgtttgag accaatgca aaaattagg gaactggcc 
      agtgttgaa aaacgagat attcagcta gaatttgcg 
      tataggagt actgtgcgt gatcttaac tcatattcg 
      aaagagtgg gatcaacta caagagcaa gcaaaggag 
      attgaagat aaacttaaa gaattagaa gcttcgcca 
      ccaagtgat gtgtacttg tcatcgaaa gatcgccag 
      attttggac tggcatttt gccaacttg gaatttgca 
      aatgcgact ccactttcg aatctatcg ctgaaacat 
      tgggatcag gacgacgat tttgaattc acggggaat 
      cacttaaca ggtgagtgg gcgcaactg taaaaactg 
      gagtgtacg gctggttgc atacatagt tccgtgtta 
      actcacgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY