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Gene information
General information
TC032013 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 4013418..4037648
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032013
    iBeetle screen iB_02679

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032013 RA
      MPLTECVIL WILLQESVL TKAPRSVAG GGAHSTEEE 
      MAKTLITYG LRNGYSCVP VALAEGLDI KLNAAVKKV 
      EYNKNGVEV TVYNPRIPH SVNTYHADV VLCTLPLGV 
      LKLCASPNS SQLNTVQFS PPLPDWKTS AIQRLGFGN 
      LNKVVLCFE RIFWNPQAN LFGHVGSTT ASRGELFLF 
      WNLYKAPVL LALVAGEAA AIMENVTDD VIVGRCIAV 
      LRGIFGQSG VPQPKETVV TRWRADPWS RGSYSFVAV 
      GSSGSDYDL LASPVIPPN SQGVSGTAG PARVFFAGE 
      HTIRNYPAT VHGAFLSGL REASRIADQ IIGNPCLPQ 
      PAE
    CDS sequence
    • >TC032013 RA
      atgccatta acggaatgc gtaatcctc tggattctc 
      ctccaagag tcggtgctc acgaaggcc ccgaggtcc 
      gtcgcgggt ggcggcgct cattccacc gaagaggag 
      atggcaaag actctaata acttatggg cttcgcaac 
      ggatattcg tgtgttccg gtggcctta gccgagggt 
      ttggacatc aaattgaat gctgcggtt aagaaggtg 
      gagtataac aagaacgga gtcgaagtc actgtttat 
      aatcctagg ataccgcat tcggtgaat acttatcac 
      gcggacgtc gtcctgtgc actcttcct ctgggagtt 
      ttaaaatta tgcgcgtca ccaaactcg tcccaactg 
      aatacagtt caattttcg ccgcctctg ccagattgg 
      aagacttcg gcaatccag aggctgggt ttcggtaat 
      ttgaataaa gtcgtcttg tgtttcgag cggattttc 
      tggaatccg caagctaat cttttcgga cacgttgga 
      agcactact gcgagccgc ggcgaattg ttccttttt 
      tggaacttg tataaagca ccggttctt cttgcgtta 
      gttgcaggt gaagcggct gctatcatg gaaaatgtc 
      accgatgat gttattgtc ggaagatgt atcgccgtg 
      cttcggggc atttttggc cagtctgga gtgccccaa 
      ccgaaagag actgtggtg acacgctgg agggctgat 
      ccctggtcg aggggttct tacagtttc gtggctgtg 
      ggcagttca ggcagtgat tacgatttg ttggcttcg 
      ccggtgatt cccccgaac agtcagggg gtttcgggg 
      actgccgga cctgcgaga gtgtttttt gcgggggaa 
      cacacaatt aggaactat cctgcgacg gtccacggg 
      gctttctta agtgggctt agggaagct agtcgaata 
      gctgatcaa attatcgga aatccgtgc cttcctcaa 
      ccggcggaa tga
    mRNA sequence
    • >TC032013 RA
      acatatatc tccggagcg gcataaacg gtgggcatt 
      tcgtgtcgg acaagtgta aattgcgcc taggtcgct 
      gtaactcat gccattaac ggaatgcgt aatcctctg 
      gattctcct ccaagagtc ggtgctcac gaaggcccc 
      gaggtccgt cgcgggtgg cggcgctca ttccaccga 
      agaggagat ggcaaagac tctaataac ttatgggct 
      tcgcaacgg atattcgtg tgttccggt ggccttagc 
      cgagggttt ggacatcaa attgaatgc tgcggttaa 
      gaaggtgga gtataacaa gaacggagt cgaagtcac 
      tgtttataa tcctaggat accgcattc ggtgaatac 
      ttatcacgc ggacgtcgt cctgtgcac tcttcctct 
      gggagtttt aaaattatg cgcgtcacc aaactcgtc 
      ccaactgaa tacagttca attttcgcc gcctctgcc 
      agattggaa gacttcggc aatccagag gctgggttt 
      cggtaattt gaataaagt cgtcttgtg tttcgagcg 
      gattttctg gaatccgca agctaatct tttcggaca 
      cgttggaag cactactgc gagccgcgg cgaattgtt 
      ccttttttg gaacttgta taaagcacc ggttcttct 
      tgcgttagt tgcaggtga agcggctgc tatcatgga 
      aaatgtcac cgatgatgt tattgtcgg aagatgtat 
      cgccgtgct tcggggcat ttttggcca gtctggagt 
      gccccaacc gaaagagac tgtggtgac acgctggag 
      ggctgatcc ctggtcgag gggttctta cagtttcgt 
      ggctgtggg cagttcagg cagtgatta cgatttgtt 
      ggcttcgcc ggtgattcc cccgaacag tcagggggt 
      ttcggggac tgccggacc tgcgagagt gttttttgc 
      gggggaaca cacaattag gaactatcc tgcgacggt 
      ccacggggc tttcttaag tgggcttag ggaagctag 
      tcgaatagc tgatcaaat tatcggaaa tccgtgcct 
      tcctcaacc ggcggaatg aattttacg aattaatgg 
      cgtagttca ttaattttt gtagagtaa ttatcggtc 
      tgaccgact gatgtgtgt gcataagat aagtaaaaa 
      cgctctata aattcgcct taattgcag tctggagcg 
      tcgtattat aaaataata agtatcgta agcttgtaa 
      gataaattc cttctgtgt tttatgcac aatttatgc 
      gtcacgtaa attatgaag ggagtattt cataaaaaa 
      cgttacaat tccaataga gcgattatt attaatcca 
      aacgaaagt ggatcacga tcatcctca ataattgca 
      acagtggta tataaatct tgcatggtt acaaagcga 
      tgttaactg taaatagca gaggtaatt tatcttcaa 
      ttggacatt tgcattgtt aattttccg aattccgtg 
      cttgaaagt gataatttg ttgttaaga tcaaatttg 
      ttatcaggt aatcaagtt agtacaaat ttttgttac 
      gtgagaagt gcactcgtc ttggctgtt gtggtgctt 
      ttttaattt aacgctttt ggaaataaa tcgtgaatg 
      acacg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY