| General information | |||
| TC005228 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 1857610..1880512 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC005228 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02678 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
larva lethality late (L7) - Number of animals showing the phenotype: 6 (11 dpi)
- >TC005228 RA
MTTTIEIPL RDTDEVIEL DSDQLPDGD EVLGILRQE NTQLFIWVN LALEYYKQG KIEDFIKIL EASRTDANV DYRDYEKDQ MRALDMLAA YYVQEANRE KNKDKKREL FTKATLLYT TADKIIMYD QNHLLGRAY FCLLEGDKM EQADAQFNF VLNQSPNNI PSLLGKACI AYNKKDFRG ALAFYKKAL RTNPNCPAA VRLGMGHCF MKLNNQEKA RLAFERALQ LDPQCVGAL VGLAILKLN LQQPESIRS GVQMLSKAY TIDSSNPMV LNHLANHFF FKKDYSKVQ HLALHAFHN TENEAMRAE SCYQLARAF HVQGDYDQA FQYYYQATQ FAPTAFVLP HFGLGQMYI YRGDAENAA QCFEKVLKA QPGNYETMK ILGSLYANS SSQSKRDIA KNHLKKVTE QFPDDIEAW IELAQILEQ SDLQGSLNA YGTAIQILN KDVQADIPT EILNNVGAL HYRLNNLEE AKKNLEEAL TRAKTEAEH DPQYYNSIS VTITYNLAR LNEALCLFD KSEKLYKDI LKERPNYVD CYLRLGCMA RDKGHIYEA SDWFKEALR FNTEHPDAW SLLGNLHLA KAEWGPGQK KYERILKNP ATSQDSYSL IVLGNVWLQ TLHQPTKDK EREKRHQER ALSMFKQVL KIDPKNIWA ANGIGAVLA HKGAVNEAR DIFAQVREA TADFCDVWL NIAHVYVEQ KQFVSAIQM YENCLRKFF KYNNVEVLQ YLARAYYKA SKLKEAKMV LLKARRVAP HDTVLLYNI ALVLQRLAT HILKDEKST LQTVLQAVH ELGLSLKYF TYLAEFGDK MRYDVTLAA MEARQCKDL LSQAQYHVA RARRVDEEE RQLRRKQED ERTAFKMRQ LEEQKKVEE EMRLTKEQL LQKRQEYKE KTKNALLFM EIPSEKTKG KGKGRKDQY ISDSGSDRE NQEGGEPKE RGRKRQRSK DRKRKGGSG RKRREKGGH SDSESDRPK NKRSRKKFK ESKGKQKDE GLSAKQKSR IVSKATIST SEDDSDDGK LKIASGDES DSRGRKRKI SSGSESRSR SRSRSRSKS GSRSRSGSR SRSRSSSRS KSGSRSRSV SRSKSRSRS RSKSGSRSR SRSRSRSKS GSRSRSRSP SRSRSRSRS RSQSGSRRS RSGSGSRSK SRSKSRSKS RSKSRSKSR SKSRSKSRS RSRSGSPEK QSGSEKGSR ASSPAGSD
- >TC005228 RA
atgactaca acaatcgaa attcctctc cgtgacacg gatgaggtt atagagctt gactctgat cagttgcct gatggagac gaagttttg ggtatttta cgacaagaa aatacccag ttgtttata tgggttaat cttgcgctg gagtattac aaacaaggt aaaattgaa gattttatt aaaatactt gaagcttca cgaactgat gccaatgtc gattaccga gactatgaa aaggatcaa atgcgagcc cttgatatg cttgccgca tattatgta caagaagcc aacagggag aaaaataag gacaaaaaa cgagagttg tttactaaa gcaactctt ctgtacacc acagctgat aaaattatt atgtacgac caaaaccat cttttggga agggcctat ttttgcctc cttgaaggc gataagatg gaacaagcg gatgcgcag tttaatttc gttttgaat cagtcacct aataatata ccgtcattg ttggggaaa gcttgcatt gcgtacaat aaaaaggat ttccgaggg gctttggct ttttacaag aaagctttg aggaccaat ccaaactgt cctgctgca gtacgacta ggaatgggt cactgtttt atgaaactg aataaccaa gaaaaagcc agattggca tttgagaga gcactccaa ctggatcca caatgtgta ggagctctg gttggctta gctatcctc aaattaaat ttacaacaa ccagaatcg attcgcagt ggtgttcag atgttatcc aaagcttac accattgat tcttccaat ccgatggtt ttgaatcat ttggccaat catttcttc ttcaaaaaa gattacagt aaagtccaa catttggca cttcatgcc ttccacaac acggaaaat gaagcaatg agggctgaa agttgttat cagttagcg agggctttc catgtacag ggtgactat gaccaagct ttccaatat tactatcag gcgacgcaa tttgcaccg actgcgttt gtcctacca catttcggg ttaggacaa atgtatatt taccgaggc gatgcagaa aatgccgcc caatgtttc gaaaaagta ttaaaagcc caacccggc aattacgaa acgatgaaa attttgggt tcactgtat gcaaactca tcgtctcag tcaaagcgc gatatcgct aagaatcat ctaaaaaaa gtaacggaa caatttccc gatgatatt gaagcatgg atcgaattg gcccaaatt ttggaacaa tccgatctt caaggctca ctcaatgca tacggaacg gcgattcaa attttaaat aaagacgtg caagctgat ataccgacc gaaatcttg aataatgta ggtgcgcta cattaccga ctgaacaat ctagaagaa gcgaagaaa aatctcgaa gaggctcta acgcgagct aaaactgaa gcagaacat gatccacag tattataat tcgatttcg gtgacgatt acttataac ctggctagg ttgaacgaa gccttgtgt ttgtttgat aaatctgaa aaattgtat aaggacata ttgaaagag cgaccaaat tatgttgat tgttatttg agactgggt tgtatggca cgtgataaa ggtcacatc tacgaagct tctgattgg tttaaggaa gctttgagg tttaataca gaacatcct gatgcgtgg tcattgctt ggtaatctt catttagct aaggcagag tggggacct ggtcagaag aaatatgaa aggatttta aagaatccg gcaacttcg caagactct tattcctta attgttttg ggaaacgtt tggttgcaa acccttcat caaccaacc aaagacaaa gaacgagaa aaacgccat caagaaaga gcattatca atgtttaaa caagtttta aaaattgac ccgaaaaac atctgggct gcaaatgga atcggagcc gttttagct cacaaagga gcggttaat gaagcacgt gatattttc gcacaagtt agagaagct accgcagat ttttgcgac gtttggttg aatattgca catgtttat gttgaacag aaacaattt gtcagcgcc atacagatg tacgaaaat tgcttgaga aaatttttc aagtataat aacgttgaa gtactccag tatttggct agagcgtat tataaagct tccaagttg aaggaagca aagatggtg ttactgaaa gcacgtcga gttgctcca catgataca gttttactg tataatatt gctttagtc ttacagagg ttagcaaca catattttg aaggacgaa aaatcgact ttacagacc gttcttcag gcggtacac gaacttggg ttatctcta aaatatttt acttatttg gctgaattt ggtgataaa atgcgctat gatgtgact ttggcagct atggaagca cgccaatgt aaagattta ctttctcaa gcgcaatat cacgttgcc agagcacga agagtagat gaagaagaa cgtcaattg agaaggaaa caagaagat gaaagaacc gcatttaag atgcgccaa cttgaagaa cagaagaaa gtggaagaa gaaatgcgt ttaaccaaa gaacagctc cttcaaaaa cgtcaagaa tacaaagag aagactaaa aacgctctc ctgtttatg gaaattcca agcgagaaa acgaaagga aaaggcaaa ggtcgcaaa gatcaatac atcagtgat tcgggaagt gatcgcgaa aatcaagaa ggtggcgaa ccgaaagaa agaggacgc aaaaggcaa cgaagcaaa gaccgaaag aggaaaggt ggcagtgga cgcaaacga agagaaaaa ggtggtcat tcagattcg gaaagtgat cgtccgaaa aataaacgc agtcgaaag aagtttaaa gaatcgaaa gggaagcag aaagatgaa ggactcagt gcgaaacaa aagagtcgg attgtttcg aaagctact atctcgact agtgaagat gatagtgat gatggaaaa ttgaagatt gcaagtggg gatgaaagt gatagtcgt ggacgaaag aggaagatt tcttcggga tcggaaagt cgttccaga tctagatct cgatccaga tcgaagagt gggtcaaga tcaagaagt ggatcccgt tcaagatca cgaagtagt tcaagatcg aaatctggt tcaagaagt agaagtgtg tcaaggtct aaatcacgt tcacgttca cgatcgaag tctggatcc agatctagg tccagatcc agaagtcga tccaaatct ggatcacgg tcacgatca cgatcgcct tcaaggtct aggtctagg tctaggtca aggtcgcaa agtgggtct agaaggtct aggtcagga agtggctct agaagtaag agtaggagc aagagtcgg agtaagagt aggagcaag agtcggagt aagagtagg agcaaaagt cggagtaag agtaggagt cggagtagg tctggatct cctgaaaag cagtcaggg agtgagaaa ggatcgaga gcgtcttca cctgctgga agtgactag
- >TC005228 RA
aacaaatat taaattacc aaacttacc aaagcgatt atctccaat gcaccacat ttatatcgc aatttcttt tcacttggc tgagagttg ttgacgaaa agcgcactg cacttgacc gtaaaacaa tggtttggt tttataaga aaagtctcg gtaaagtgg cgaataatt gaacacctc ttgataaga acagtggat tggaagttt aaagaacgc gtgttcggc gcgaactgt acagacgac gctgaaatt tgtggcttg tgcatcatc caattttca atttccgat ttttagtga aaaatgccc ttttcatga aatatatgt gacgtgaag tgttaacaa actcttcaa aatgactac aacaatcga aattcctct ccgtgacac ggatgaggt tatagagct tgactctga tcagttgcc tgatggaga cgaagtttt gggtatttt acgacaaga aaataccca gttgtttat atgggttaa tcttgcgct ggagtatta caaacaagg taaaattga agattttat taaaatact tgaagcttc acgaactga tgccaatgt cgattaccg agactatga aaaggatca aatgcgagc ccttgatat gcttgccgc atattatgt acaagaagc caacaggga gaaaaataa ggacaaaaa acgagagtt gtttactaa agcaactct tctgtacac cacagctga taaaattat tatgtacga ccaaaacca tcttttggg aagggccta tttttgcct ccttgaagg cgataagat ggaacaagc ggatgcgca gtttaattt cgttttgaa tcagtcacc taataatat accgtcatt gttggggaa agcttgcat tgcgtacaa taaaaagga tttccgagg ggctttggc tttttacaa gaaagcttt gaggaccaa tccaaactg tcctgctgc agtacgact aggaatggg tcactgttt tatgaaact gaataacca agaaaaagc cagattggc atttgagag agcactcca actggatcc acaatgtgt aggagctct ggttggctt agctatcct caaattaaa tttacaaca accagaatc gattcgcag tggtgttca gatgttatc caaagctta caccattga ttcttccaa tccgatggt tttgaatca tttggccaa tcatttctt cttcaaaaa agattacag taaagtcca acatttggc acttcatgc cttccacaa cacggaaaa tgaagcaat gagggctga aagttgtta tcagttagc gagggcttt ccatgtaca gggtgacta tgaccaagc tttccaata ttactatca ggcgacgca atttgcacc gactgcgtt tgtcctacc acatttcgg gttaggaca aatgtatat ttaccgagg cgatgcaga aaatgccgc ccaatgttt cgaaaaagt attaaaagc ccaacccgg caattacga aacgatgaa aattttggg ttcactgta tgcaaactc atcgtctca gtcaaagcg cgatatcgc taagaatca tctaaaaaa agtaacgga acaatttcc cgatgatat tgaagcatg gatcgaatt ggcccaaat tttggaaca atccgatct tcaaggctc actcaatgc atacggaac ggcgattca aattttaaa taaagacgt gcaagctga tataccgac cgaaatctt gaataatgt aggtgcgct acattaccg actgaacaa tctagaaga agcgaagaa aaatctcga agaggctct aacgcgagc taaaactga agcagaaca tgatccaca gtattataa ttcgatttc ggtgacgat tacttataa cctggctag gttgaacga agccttgtg tttgtttga taaatctga aaaattgta taaggacat attgaaaga gcgaccaaa ttatgttga ttgttattt gagactggg ttgtatggc acgtgataa aggtcacat ctacgaagc ttctgattg gtttaagga agctttgag gtttaatac agaacatcc tgatgcgtg gtcattgct tggtaatct tcatttagc taaggcaga gtggggacc tggtcagaa gaaatatga aaggatttt aaagaatcc ggcaacttc gcaagactc ttattcctt aattgtttt gggaaacgt ttggttgca aacccttca tcaaccaac caaagacaa agaacgaga aaaacgcca tcaagaaag agcattatc aatgtttaa acaagtttt aaaaattga cccgaaaaa catctgggc tgcaaatgg aatcggagc cgttttagc tcacaaagg agcggttaa tgaagcacg tgatatttt cgcacaagt tagagaagc taccgcaga tttttgcga cgtttggtt gaatattgc acatgttta tgttgaaca gaaacaatt tgtcagcgc catacagat gtacgaaaa ttgcttgag aaaattttt caagtataa taacgttga agtactcca gtatttggc tagagcgta ttataaagc ttccaagtt gaaggaagc aaagatggt gttactgaa agcacgtcg agttgctcc acatgatac agttttact gtataatat tgctttagt cttacagag gttagcaac acatatttt gaaggacga aaaatcgac tttacagac cgttcttca ggcggtaca cgaacttgg gttatctct aaaatattt tacttattt ggctgaatt tggtgataa aatgcgcta tgatgtgac tttggcagc tatggaagc acgccaatg taaagattt actttctca agcgcaata tcacgttgc cagagcacg aagagtaga tgaagaaga acgtcaatt gagaaggaa acaagaaga tgaaagaac cgcatttaa gatgcgcca acttgaaga acagaagaa agtggaaga agaaatgcg tttaaccaa agaacagct ccttcaaaa acgtcaaga atacaaaga gaagactaa aaacgctct cctgtttat ggaaattcc aagcgagaa aacgaaagg aaaaggcaa aggtcgcaa agatcaata catcagtga ttcgggaag tgatcgcga aaatcaaga aggtggcga accgaaaga aagaggacg caaaaggca acgaagcaa agaccgaaa gaggaaagg tggcagtgg acgcaaacg aagagaaaa aggtggtca ttcagattc ggaaagtga tcgtccgaa aaataaacg cagtcgaaa gaagtttaa agaatcgaa agggaagca gaaagatga aggactcag tgcgaaaca aaagagtcg gattgtttc gaaagctac tatctcgac tagtgaaga tgatagtga tgatggaaa attgaagat tgcaagtgg ggatgaaag tgatagtcg tggacgaaa gaggaagat ttcttcggg atcggaaag tcgttccag atctagatc tcgatccag atcgaagag tgggtcaag atcaagaag tggatcccg ttcaagatc acgaagtag ttcaagatc gaaatctgg ttcaagaag tagaagtgt gtcaaggtc taaatcacg ttcacgttc acgatcgaa gtctggatc cagatctag gtccagatc cagaagtcg atccaaatc tggatcacg gtcacgatc acgatcgcc ttcaaggtc taggtctag gtctaggtc aaggtcgca aagtgggtc tagaaggtc taggtcagg aagtggctc tagaagtaa gagtaggag caagagtcg gagtaagag taggagcaa gagtcggag taagagtag gagcaaaag tcggagtaa gagtaggag tcggagtag gtctggatc tcctgaaaa gcagtcagg gagtgagaa aggatcgag agcgtcttc acctgctgg aagtgacta ggttaaggt tagctttat ggtagaaca tagtaatat taattatga acaagtaaa taaaaactt ttattaaac
