Status
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Gene information
General information
TC005228 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 1857610..1880512
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005228
    iBeetle screen iB_02678

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 9 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005228 RA
      MTTTIEIPL RDTDEVIEL DSDQLPDGD EVLGILRQE 
      NTQLFIWVN LALEYYKQG KIEDFIKIL EASRTDANV 
      DYRDYEKDQ MRALDMLAA YYVQEANRE KNKDKKREL 
      FTKATLLYT TADKIIMYD QNHLLGRAY FCLLEGDKM 
      EQADAQFNF VLNQSPNNI PSLLGKACI AYNKKDFRG 
      ALAFYKKAL RTNPNCPAA VRLGMGHCF MKLNNQEKA 
      RLAFERALQ LDPQCVGAL VGLAILKLN LQQPESIRS 
      GVQMLSKAY TIDSSNPMV LNHLANHFF FKKDYSKVQ 
      HLALHAFHN TENEAMRAE SCYQLARAF HVQGDYDQA 
      FQYYYQATQ FAPTAFVLP HFGLGQMYI YRGDAENAA 
      QCFEKVLKA QPGNYETMK ILGSLYANS SSQSKRDIA 
      KNHLKKVTE QFPDDIEAW IELAQILEQ SDLQGSLNA 
      YGTAIQILN KDVQADIPT EILNNVGAL HYRLNNLEE 
      AKKNLEEAL TRAKTEAEH DPQYYNSIS VTITYNLAR 
      LNEALCLFD KSEKLYKDI LKERPNYVD CYLRLGCMA 
      RDKGHIYEA SDWFKEALR FNTEHPDAW SLLGNLHLA 
      KAEWGPGQK KYERILKNP ATSQDSYSL IVLGNVWLQ 
      TLHQPTKDK EREKRHQER ALSMFKQVL KIDPKNIWA 
      ANGIGAVLA HKGAVNEAR DIFAQVREA TADFCDVWL 
      NIAHVYVEQ KQFVSAIQM YENCLRKFF KYNNVEVLQ 
      YLARAYYKA SKLKEAKMV LLKARRVAP HDTVLLYNI 
      ALVLQRLAT HILKDEKST LQTVLQAVH ELGLSLKYF 
      TYLAEFGDK MRYDVTLAA MEARQCKDL LSQAQYHVA 
      RARRVDEEE RQLRRKQED ERTAFKMRQ LEEQKKVEE 
      EMRLTKEQL LQKRQEYKE KTKNALLFM EIPSEKTKG 
      KGKGRKDQY ISDSGSDRE NQEGGEPKE RGRKRQRSK 
      DRKRKGGSG RKRREKGGH SDSESDRPK NKRSRKKFK 
      ESKGKQKDE GLSAKQKSR IVSKATIST SEDDSDDGK 
      LKIASGDES DSRGRKRKI SSGSESRSR SRSRSRSKS 
      GSRSRSGSR SRSRSSSRS KSGSRSRSV SRSKSRSRS 
      RSKSGSRSR SRSRSRSKS GSRSRSRSP SRSRSRSRS 
      RSQSGSRRS RSGSGSRSK SRSKSRSKS RSKSRSKSR 
      SKSRSKSRS RSRSGSPEK QSGSEKGSR ASSPAGSD
    CDS sequence
    • >TC005228 RA
      atgactaca acaatcgaa attcctctc cgtgacacg 
      gatgaggtt atagagctt gactctgat cagttgcct 
      gatggagac gaagttttg ggtatttta cgacaagaa 
      aatacccag ttgtttata tgggttaat cttgcgctg 
      gagtattac aaacaaggt aaaattgaa gattttatt 
      aaaatactt gaagcttca cgaactgat gccaatgtc 
      gattaccga gactatgaa aaggatcaa atgcgagcc 
      cttgatatg cttgccgca tattatgta caagaagcc 
      aacagggag aaaaataag gacaaaaaa cgagagttg 
      tttactaaa gcaactctt ctgtacacc acagctgat 
      aaaattatt atgtacgac caaaaccat cttttggga 
      agggcctat ttttgcctc cttgaaggc gataagatg 
      gaacaagcg gatgcgcag tttaatttc gttttgaat 
      cagtcacct aataatata ccgtcattg ttggggaaa 
      gcttgcatt gcgtacaat aaaaaggat ttccgaggg 
      gctttggct ttttacaag aaagctttg aggaccaat 
      ccaaactgt cctgctgca gtacgacta ggaatgggt 
      cactgtttt atgaaactg aataaccaa gaaaaagcc 
      agattggca tttgagaga gcactccaa ctggatcca 
      caatgtgta ggagctctg gttggctta gctatcctc 
      aaattaaat ttacaacaa ccagaatcg attcgcagt 
      ggtgttcag atgttatcc aaagcttac accattgat 
      tcttccaat ccgatggtt ttgaatcat ttggccaat 
      catttcttc ttcaaaaaa gattacagt aaagtccaa 
      catttggca cttcatgcc ttccacaac acggaaaat 
      gaagcaatg agggctgaa agttgttat cagttagcg 
      agggctttc catgtacag ggtgactat gaccaagct 
      ttccaatat tactatcag gcgacgcaa tttgcaccg 
      actgcgttt gtcctacca catttcggg ttaggacaa 
      atgtatatt taccgaggc gatgcagaa aatgccgcc 
      caatgtttc gaaaaagta ttaaaagcc caacccggc 
      aattacgaa acgatgaaa attttgggt tcactgtat 
      gcaaactca tcgtctcag tcaaagcgc gatatcgct 
      aagaatcat ctaaaaaaa gtaacggaa caatttccc 
      gatgatatt gaagcatgg atcgaattg gcccaaatt 
      ttggaacaa tccgatctt caaggctca ctcaatgca 
      tacggaacg gcgattcaa attttaaat aaagacgtg 
      caagctgat ataccgacc gaaatcttg aataatgta 
      ggtgcgcta cattaccga ctgaacaat ctagaagaa 
      gcgaagaaa aatctcgaa gaggctcta acgcgagct 
      aaaactgaa gcagaacat gatccacag tattataat 
      tcgatttcg gtgacgatt acttataac ctggctagg 
      ttgaacgaa gccttgtgt ttgtttgat aaatctgaa 
      aaattgtat aaggacata ttgaaagag cgaccaaat 
      tatgttgat tgttatttg agactgggt tgtatggca 
      cgtgataaa ggtcacatc tacgaagct tctgattgg 
      tttaaggaa gctttgagg tttaataca gaacatcct 
      gatgcgtgg tcattgctt ggtaatctt catttagct 
      aaggcagag tggggacct ggtcagaag aaatatgaa 
      aggatttta aagaatccg gcaacttcg caagactct 
      tattcctta attgttttg ggaaacgtt tggttgcaa 
      acccttcat caaccaacc aaagacaaa gaacgagaa 
      aaacgccat caagaaaga gcattatca atgtttaaa 
      caagtttta aaaattgac ccgaaaaac atctgggct 
      gcaaatgga atcggagcc gttttagct cacaaagga 
      gcggttaat gaagcacgt gatattttc gcacaagtt 
      agagaagct accgcagat ttttgcgac gtttggttg 
      aatattgca catgtttat gttgaacag aaacaattt 
      gtcagcgcc atacagatg tacgaaaat tgcttgaga 
      aaatttttc aagtataat aacgttgaa gtactccag 
      tatttggct agagcgtat tataaagct tccaagttg 
      aaggaagca aagatggtg ttactgaaa gcacgtcga 
      gttgctcca catgataca gttttactg tataatatt 
      gctttagtc ttacagagg ttagcaaca catattttg 
      aaggacgaa aaatcgact ttacagacc gttcttcag 
      gcggtacac gaacttggg ttatctcta aaatatttt 
      acttatttg gctgaattt ggtgataaa atgcgctat 
      gatgtgact ttggcagct atggaagca cgccaatgt 
      aaagattta ctttctcaa gcgcaatat cacgttgcc 
      agagcacga agagtagat gaagaagaa cgtcaattg 
      agaaggaaa caagaagat gaaagaacc gcatttaag 
      atgcgccaa cttgaagaa cagaagaaa gtggaagaa 
      gaaatgcgt ttaaccaaa gaacagctc cttcaaaaa 
      cgtcaagaa tacaaagag aagactaaa aacgctctc 
      ctgtttatg gaaattcca agcgagaaa acgaaagga 
      aaaggcaaa ggtcgcaaa gatcaatac atcagtgat 
      tcgggaagt gatcgcgaa aatcaagaa ggtggcgaa 
      ccgaaagaa agaggacgc aaaaggcaa cgaagcaaa 
      gaccgaaag aggaaaggt ggcagtgga cgcaaacga 
      agagaaaaa ggtggtcat tcagattcg gaaagtgat 
      cgtccgaaa aataaacgc agtcgaaag aagtttaaa 
      gaatcgaaa gggaagcag aaagatgaa ggactcagt 
      gcgaaacaa aagagtcgg attgtttcg aaagctact 
      atctcgact agtgaagat gatagtgat gatggaaaa 
      ttgaagatt gcaagtggg gatgaaagt gatagtcgt 
      ggacgaaag aggaagatt tcttcggga tcggaaagt 
      cgttccaga tctagatct cgatccaga tcgaagagt 
      gggtcaaga tcaagaagt ggatcccgt tcaagatca 
      cgaagtagt tcaagatcg aaatctggt tcaagaagt 
      agaagtgtg tcaaggtct aaatcacgt tcacgttca 
      cgatcgaag tctggatcc agatctagg tccagatcc 
      agaagtcga tccaaatct ggatcacgg tcacgatca 
      cgatcgcct tcaaggtct aggtctagg tctaggtca 
      aggtcgcaa agtgggtct agaaggtct aggtcagga 
      agtggctct agaagtaag agtaggagc aagagtcgg 
      agtaagagt aggagcaag agtcggagt aagagtagg 
      agcaaaagt cggagtaag agtaggagt cggagtagg 
      tctggatct cctgaaaag cagtcaggg agtgagaaa 
      ggatcgaga gcgtcttca cctgctgga agtgactag
    mRNA sequence
    • >TC005228 RA
      aacaaatat taaattacc aaacttacc aaagcgatt 
      atctccaat gcaccacat ttatatcgc aatttcttt 
      tcacttggc tgagagttg ttgacgaaa agcgcactg 
      cacttgacc gtaaaacaa tggtttggt tttataaga 
      aaagtctcg gtaaagtgg cgaataatt gaacacctc 
      ttgataaga acagtggat tggaagttt aaagaacgc 
      gtgttcggc gcgaactgt acagacgac gctgaaatt 
      tgtggcttg tgcatcatc caattttca atttccgat 
      ttttagtga aaaatgccc ttttcatga aatatatgt 
      gacgtgaag tgttaacaa actcttcaa aatgactac 
      aacaatcga aattcctct ccgtgacac ggatgaggt 
      tatagagct tgactctga tcagttgcc tgatggaga 
      cgaagtttt gggtatttt acgacaaga aaataccca 
      gttgtttat atgggttaa tcttgcgct ggagtatta 
      caaacaagg taaaattga agattttat taaaatact 
      tgaagcttc acgaactga tgccaatgt cgattaccg 
      agactatga aaaggatca aatgcgagc ccttgatat 
      gcttgccgc atattatgt acaagaagc caacaggga 
      gaaaaataa ggacaaaaa acgagagtt gtttactaa 
      agcaactct tctgtacac cacagctga taaaattat 
      tatgtacga ccaaaacca tcttttggg aagggccta 
      tttttgcct ccttgaagg cgataagat ggaacaagc 
      ggatgcgca gtttaattt cgttttgaa tcagtcacc 
      taataatat accgtcatt gttggggaa agcttgcat 
      tgcgtacaa taaaaagga tttccgagg ggctttggc 
      tttttacaa gaaagcttt gaggaccaa tccaaactg 
      tcctgctgc agtacgact aggaatggg tcactgttt 
      tatgaaact gaataacca agaaaaagc cagattggc 
      atttgagag agcactcca actggatcc acaatgtgt 
      aggagctct ggttggctt agctatcct caaattaaa 
      tttacaaca accagaatc gattcgcag tggtgttca 
      gatgttatc caaagctta caccattga ttcttccaa 
      tccgatggt tttgaatca tttggccaa tcatttctt 
      cttcaaaaa agattacag taaagtcca acatttggc 
      acttcatgc cttccacaa cacggaaaa tgaagcaat 
      gagggctga aagttgtta tcagttagc gagggcttt 
      ccatgtaca gggtgacta tgaccaagc tttccaata 
      ttactatca ggcgacgca atttgcacc gactgcgtt 
      tgtcctacc acatttcgg gttaggaca aatgtatat 
      ttaccgagg cgatgcaga aaatgccgc ccaatgttt 
      cgaaaaagt attaaaagc ccaacccgg caattacga 
      aacgatgaa aattttggg ttcactgta tgcaaactc 
      atcgtctca gtcaaagcg cgatatcgc taagaatca 
      tctaaaaaa agtaacgga acaatttcc cgatgatat 
      tgaagcatg gatcgaatt ggcccaaat tttggaaca 
      atccgatct tcaaggctc actcaatgc atacggaac 
      ggcgattca aattttaaa taaagacgt gcaagctga 
      tataccgac cgaaatctt gaataatgt aggtgcgct 
      acattaccg actgaacaa tctagaaga agcgaagaa 
      aaatctcga agaggctct aacgcgagc taaaactga 
      agcagaaca tgatccaca gtattataa ttcgatttc 
      ggtgacgat tacttataa cctggctag gttgaacga 
      agccttgtg tttgtttga taaatctga aaaattgta 
      taaggacat attgaaaga gcgaccaaa ttatgttga 
      ttgttattt gagactggg ttgtatggc acgtgataa 
      aggtcacat ctacgaagc ttctgattg gtttaagga 
      agctttgag gtttaatac agaacatcc tgatgcgtg 
      gtcattgct tggtaatct tcatttagc taaggcaga 
      gtggggacc tggtcagaa gaaatatga aaggatttt 
      aaagaatcc ggcaacttc gcaagactc ttattcctt 
      aattgtttt gggaaacgt ttggttgca aacccttca 
      tcaaccaac caaagacaa agaacgaga aaaacgcca 
      tcaagaaag agcattatc aatgtttaa acaagtttt 
      aaaaattga cccgaaaaa catctgggc tgcaaatgg 
      aatcggagc cgttttagc tcacaaagg agcggttaa 
      tgaagcacg tgatatttt cgcacaagt tagagaagc 
      taccgcaga tttttgcga cgtttggtt gaatattgc 
      acatgttta tgttgaaca gaaacaatt tgtcagcgc 
      catacagat gtacgaaaa ttgcttgag aaaattttt 
      caagtataa taacgttga agtactcca gtatttggc 
      tagagcgta ttataaagc ttccaagtt gaaggaagc 
      aaagatggt gttactgaa agcacgtcg agttgctcc 
      acatgatac agttttact gtataatat tgctttagt 
      cttacagag gttagcaac acatatttt gaaggacga 
      aaaatcgac tttacagac cgttcttca ggcggtaca 
      cgaacttgg gttatctct aaaatattt tacttattt 
      ggctgaatt tggtgataa aatgcgcta tgatgtgac 
      tttggcagc tatggaagc acgccaatg taaagattt 
      actttctca agcgcaata tcacgttgc cagagcacg 
      aagagtaga tgaagaaga acgtcaatt gagaaggaa 
      acaagaaga tgaaagaac cgcatttaa gatgcgcca 
      acttgaaga acagaagaa agtggaaga agaaatgcg 
      tttaaccaa agaacagct ccttcaaaa acgtcaaga 
      atacaaaga gaagactaa aaacgctct cctgtttat 
      ggaaattcc aagcgagaa aacgaaagg aaaaggcaa 
      aggtcgcaa agatcaata catcagtga ttcgggaag 
      tgatcgcga aaatcaaga aggtggcga accgaaaga 
      aagaggacg caaaaggca acgaagcaa agaccgaaa 
      gaggaaagg tggcagtgg acgcaaacg aagagaaaa 
      aggtggtca ttcagattc ggaaagtga tcgtccgaa 
      aaataaacg cagtcgaaa gaagtttaa agaatcgaa 
      agggaagca gaaagatga aggactcag tgcgaaaca 
      aaagagtcg gattgtttc gaaagctac tatctcgac 
      tagtgaaga tgatagtga tgatggaaa attgaagat 
      tgcaagtgg ggatgaaag tgatagtcg tggacgaaa 
      gaggaagat ttcttcggg atcggaaag tcgttccag 
      atctagatc tcgatccag atcgaagag tgggtcaag 
      atcaagaag tggatcccg ttcaagatc acgaagtag 
      ttcaagatc gaaatctgg ttcaagaag tagaagtgt 
      gtcaaggtc taaatcacg ttcacgttc acgatcgaa 
      gtctggatc cagatctag gtccagatc cagaagtcg 
      atccaaatc tggatcacg gtcacgatc acgatcgcc 
      ttcaaggtc taggtctag gtctaggtc aaggtcgca 
      aagtgggtc tagaaggtc taggtcagg aagtggctc 
      tagaagtaa gagtaggag caagagtcg gagtaagag 
      taggagcaa gagtcggag taagagtag gagcaaaag 
      tcggagtaa gagtaggag tcggagtag gtctggatc 
      tcctgaaaa gcagtcagg gagtgagaa aggatcgag 
      agcgtcttc acctgctgg aagtgacta ggttaaggt 
      tagctttat ggtagaaca tagtaatat taattatga 
      acaagtaaa taaaaactt ttattaaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY