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Gene information
General information
TC004531 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 3073163..3074817
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004531
    iBeetle screen iB_02676

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 3
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004531 RA
      MVNYNFNKT LSTERLVHQ TDENYKVSF FKMFKSLIV 
      TLLLGGNII YVQYLLFYH DEINWSYPE PAYLTTLVI 
      YFLSAAINM CFSIRIHKM SENSNDSCN FVTKLIVTG 
      VLIAVHIYF SVFMHANLQ KSETNSSGF DKVVTIFVA 
      VIFLFLQIT SFLNHVSVA QYAVQCRFA SSDSKRIKF 
      FKQMVEAEA DADLISTLD CYLRAAPQL IVQFAAIVH 
      NKWSFDQIP VLQIFFIFI AFAITPWSI TSFNGSLRK 
      QQRCEIRWK AKIIYYLWH FFVMGSRIA GVGAVAAFW 
      PSQTKILTV IHWTLMTTW LLTTVKPTH FSNFNNFWH 
      FLFCSVFGA VYFFNPVNL GNQPTRAKY LCFYLIMFV 
      ENVVANVGW FVADNEVTP VWKIAFVAF NQFLFVFGI 
      GFMVTYYVK FHPSNTQKG APQNVEPLQ ETAV
    CDS sequence
    • >TC004531 RA
      atggtgaac tataacttc aacaaaact ctttctacg 
      gaaagactg gtgcaccaa acggacgaa aattacaaa 
      gtttcgttc ttcaaaatg tttaagagt cttattgtg 
      actctcctt ctcggagga aacataatc tacgtgcaa 
      tacctcctg ttctaccat gatgaaatc aactggagt 
      tatcccgag ccggcatat ctcacaact ctcgtaatt 
      tacttcctc agcgctgcc ataaatatg tgcttcagc 
      attcgcatc cacaaaatg agcgaaaac tcaaacgat 
      tcgtgcaat ttcgtgaca aaactcata gtgaccggt 
      gtacttatc gcagttcat atttatttt tccgtgttc 
      atgcacgcg aatctccaa aaaagtgag acaaactca 
      agcgggttc gacaaagtg gttacaatt ttcgtggct 
      gtgatcttc ctatttctc cagattaca tcatttctg 
      aaccacgtt tcagtggct cagtatgct gtccagtgc 
      agattcgct tctagcgat tcaaagcga atcaaattt 
      tttaaacaa atggtggag gcggaagcc gatgctgat 
      ctgatcagc accctcgac tgctatctg cgtgctgct 
      cctcaactc attgtccaa tttgctgca atcgtccac 
      aacaaatgg agtttcgac caaatcccc gttcttcaa 
      atatttttt attttcatc gctttcgca ataacaccc 
      tggtccatc acctcgttt aatggctcc ctccgaaag 
      caacagcgt tgtgaaatc aggtggaaa gccaaaata 
      atttactac ctgtggcat tttttcgtc atgggttcc 
      cgcattgct ggtgttggg gctgttgcg gctttttgg 
      ccatcacag acgaaaatc ctgactgtg attcattgg 
      actctcatg acaacttgg ctcctaacc accgttaaa 
      ccaactcac ttttccaac tttaataat ttctggcat 
      tttctgttc tgttcagtt ttcggagcc gtgtatttt 
      tttaatcct gtgaatctc ggaaatcaa ccaacacga 
      gcgaaatat ctttgcttt tatctcata atgtttgtg 
      gaaaatgtt gtagctaat gttggttgg tttgttgca 
      gacaacgaa gttactccc gtttggaaa atagcgttc 
      gttgctttt aatcaattc ctttttgtg ttcgggatt 
      ggttttatg gtaacttat tacgttaaa ttccaccca 
      agcaatact caaaagggt gcgcctcaa aatgttgaa 
      cctcttcaa gagactgct gtttaa
    mRNA sequence
    • >TC004531 RA
      gtggatgag tgtagcagc agaaaatag ataaagtga 
      aaaaatggt gaactataa cttcaacaa aactctttc 
      tacggaaag actggtgca ccaaacgga cgaaaatta 
      caaagtttc gttcttcaa aatgtttaa gagtcttat 
      tgtgactct ccttctcgg aggaaacat aatctacgt 
      gcaatacct cctgttcta ccatgatga aatcaactg 
      gagttatcc cgagccggc atatctcac aactctcgt 
      aatttactt cctcagcgc tgccataaa tatgtgctt 
      cagcattcg catccacaa aatgagcga aaactcaaa 
      cgattcgtg caatttcgt gacaaaact catagtgac 
      cggtgtact tatcgcagt tcatattta tttttccgt 
      gttcatgca cgcgaatct ccaaaaaag tgagacaaa 
      ctcaagcgg gttcgacaa agtggttac aattttcgt 
      ggctgtgat cttcctatt tctccagat tacatcatt 
      tctgaacca cgtttcagt ggctcagta tgctgtcca 
      gtgcagatt cgcttctag cgattcaaa gcgaatcaa 
      attttttaa acaaatggt ggaggcgga agccgatgc 
      tgatctgat cagcaccct cgactgcta tctgcgtgc 
      tgctcctca actcattgt ccaatttgc tgcaatcgt 
      ccacaacaa atggagttt cgaccaaat ccccgttct 
      tcaaatatt ttttatttt catcgcttt cgcaataac 
      accctggtc catcacctc gtttaatgg ctccctccg 
      aaagcaaca gcgttgtga aatcaggtg gaaagccaa 
      aataattta ctacctgtg gcatttttt cgtcatggg 
      ttcccgcat tgctggtgt tggggctgt tgcggcttt 
      ttggccatc acagacgaa aatcctgac tgtgattca 
      ttggactct catgacaac ttggctcct aaccaccgt 
      taaaccaac tcacttttc caactttaa taatttctg 
      gcattttct gttctgttc agttttcgg agccgtgta 
      tttttttaa tcctgtgaa tctcggaaa tcaaccaac 
      acgagcgaa atatctttg cttttatct cataatgtt 
      tgtggaaaa tgttgtagc taatgttgg ttggtttgt 
      tgcagacaa cgaagttac tcccgtttg gaaaatagc 
      gttcgttgc ttttaatca attcctttt tgtgttcgg 
      gattggttt tatggtaac ttattacgt taaattcca 
      cccaagcaa tactcaaaa gggtgcgcc tcaaaatgt 
      tgaacctct tcaagagac tgctgttta atgtgattt 
      gataggtaa caaatctgt gttcagtat tgattgccg 
      ttttttaca gaaatgatg taaattaga tgaattttt 
      taacagtgc cattactaa cacaaatga tgtgataat 
      agtctacat atcataatt agatgttgt tgcctactg 
      ttatttata ataatttat tatatttca aattgaatt 
      attgcataa attaggtat attgaatat aagatacct 
      aagttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY