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Gene information
General information
TC001370 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid12: 84515..87965
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001370
    iBeetle screen iB_02674
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Fertility
    ovary size decreased - Number of animals showing the phenotype: 3

    vitellogenic egg chamber late not present - Number of animals showing the phenotype: 3

    egg chamber sometimesstructure irregular - Number of animals showing the phenotype: 1

    yolk pattern irregular - Number of animals showing the phenotype: 3

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    Pupal morphology
    wings shortened - Number of animals showing the phenotype: 2

    wings not closed - Number of animals showing the phenotype: 2
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001370 RA
      MLKNIRLRP LVNFYKYST QPVAKETPN YDNQIKEFE 
      RISKLQQKV RPKKPQRAP FVKNLFLGV FDTDLLTYP 
      EITNEELNN LNKALQPIQ TFVQQPEIK DCNKITRDF 
      LKKLSNAQL LGLQAAQLN NGRELTVTE MCRFFEVLS 
      ELKIRNLLI HNEVLAIQT LVKFGSDQL KHKYLEKLV 
      KGEATSAFC VLESKSKIG ERTGVKNLD GKTWVLNGE 
      RKSVLNGSN ADFYIVMAN TQNNTRDEI TEKKFSFFV 
      VERDFGGIT STSCGGNDL SDVIFENVT VPHENTIGG 
      ENCGEEILS TIYPQYRLS TGPLCSVLV KNLINNVTE 
      HFIRVSKKP NLLYETDAI RTKLGQLTT SLYAIDSMV 
      YFTSSLVDD YENQDSELE TSIVKVFAS EQAFLNTSA 
      CLDLIAPDS VFDNHWCKQ LHAEALSQV ILNDSNDNL 
      KILIALFGL RHAGEQLNE TIKKIRNPF YFSTYAFKR 
      MWTRRRIAE DNPKLNLYL HHYLHPSSM LASQQLEYC 
      VLKLQYATE TLLARHGPE VLNKHQDLR RLADAIIDI 
      YAMVACISR SSRSYCIGL QHADYEMVL ANAFTLGAL 
      ERVKLNLNK IIEGPIGTN DENYRMLAK RLFELKQYY 
      PVHPLSRNF
    CDS sequence
    • >TC001370 RA
      atgttaaaa aacattaga ttacgccct ttagtcaat 
      ttttacaaa tattcaact caaccggtg gctaaagaa 
      acgcccaat tatgacaat cagatcaaa gaattcgaa 
      cgaatttcg aagttgcaa caaaaagtc agaccaaaa 
      aaaccccag agagcacct tttgtcaag aaccttttt 
      ttgggggtg tttgatacc gaccttttg acctatccg 
      gaaatcaca aatgaggaa ttaaacaat ttaaacaaa 
      gctttacaa ccaattcaa actttcgtc caacaaccg 
      gaaatcaaa gattgcaat aaaatcaca cgagacttt 
      ttgaaaaaa ctgtcaaat gcccaactt ttggggctt 
      caagcggcg caattaaac aacggacga gaactaaca 
      gtaaccgaa atgtgtcgt ttttttgaa gttttatcc 
      gaacttaaa ataagaaat ctcctaata cataacgaa 
      gtcttggca atacaaact ttagtaaaa tttggttcg 
      gatcaattg aaacacaag tatttagaa aaactagtt 
      aagggtgaa gcaacttcg gcgttttgt gttcttgaa 
      tcaaaaagc aaaataggg gaaagaaca ggtgtgaaa 
      aatctagat ggcaaaact tgggtttta aacggtgaa 
      agaaaatca gttttgaac ggcagtaat gccgatttt 
      tacatcgta atggcaaac acacaaaac aacacaagg 
      gacgaaatt accgaaaaa aagttcagc ttttttgta 
      gttgagcgc gattttggg ggaatcact agtacaagt 
      tgtggcggc aatgatttg tctgatgtt atttttgaa 
      aatgttaca gtaccacac gaaaatacc atcggtggt 
      gaaaattgt ggagaagag attttatca acaatttat 
      ccacaatat cgcctcagt accggccct ctatgttca 
      gttttagtc aaaaattta ataaataat gtcaccgaa 
      cattttata agagtttca aaaaaacca aacttgttg 
      tacgaaacc gacgccatt cgtacgaaa ttaggacaa 
      ctaacaacg tcactgtat gccattgac agtatggtg 
      tattttacg tcaagtctt gtagatgat tacgaaaat 
      caagactca gagctagaa acgtcaatt gttaaggtt 
      tttgcttca gagcaagct tttctcaat acgtcagct 
      tgtctcgac ttaatcgcc cctgattca gtttttgat 
      aatcactgg tgtaaacaa ctgcatgcg gaagctttg 
      tctcaagtg attttaaac gattctaat gataattta 
      aaaattttg attgcttta tttggacta cgtcacgct 
      ggggaacaa cttaatgaa acaattaaa aaaattcga 
      aacccgttt tactttagc acttatgca tttaaacgc 
      atgtggaca cggcgccgc attgctgag gataaccca 
      aaattaaat ctatatttg catcattat ttgcaccct 
      tcatctatg cttgcttcg caacaattg gagtattgt 
      gttttaaag cttcaatac gccactgaa acgttacta 
      gcacgtcac ggaccggaa gttttgaac aaacatcaa 
      gatttaaga cgtttagct gacgctatt attgacata 
      tatgcaatg gttgcttgt atcagtcgt tcgtcgcgc 
      tcgtattgt ataggttta caacacgcc gactatgag 
      atggtttta gccaatgct ttcacactt ggagccctt 
      gaacgagtc aaattgaac ttgaacaaa ataatagaa 
      ggtccaatt ggcacaaac gacgagaat tatagaatg 
      ttggcaaag agattgttt gaattaaaa cagtactat 
      ccggtgcac ccattaagc cgcaatttt tag
    mRNA sequence
    • >TC001370 RA
      gggaaactc caagatttt cacaatgtt actatttac 
      ttgggctac gctaacaat ccattcggt gcttcctca 
      ccggctgct ttacctcag aaaaaacct cggattgat 
      gaatttcgg ccaaagcgt ggcgtaatc catctttac 
      aacatggtc agttgtgca acaggatgt aatccacta 
      atttacaag ggttacggc tgctattgt ggctttcta 
      ggctcagga tatgctgtt gatggcata gacagctgt 
      tgtaaaatg cacgattgg tgttatgac actgctaat 
      tgtcccatg tttcttgag tatttcgtg ccgtactat 
      tggaagtgt taccggaat cgccccttg tgcggtatg 
      taactgttt agagttttt ttcttctat taaataggt 
      actgtagct ctagatcag cggggaggt cccggttca 
      tgtgctcaa agattatgc gaatgtgac cgcatttta 
      tcacaatgc cttagccag tttccatgt ccctcgaaa 
      agagcctat tgtagggct tcgcattgg agactattc 
      cagaatttg ctttataat aaagtgaca tttttatga 
      catttgttt ttgacacaa atgaataaa ttactagaa 
      gttaagttt caattaaag aatactgta ccagaatcg 
      ttgtccgaa gtccaaatt gaacttccg gttaaaaaa 
      caacaagac gaagccaaa agcgttaca actgtagtt 
      ttctattgc tcgattctt ggaattgtg actgggctt 
      ataacaaca attttgggc ttcttatac tggtttaaa 
      gtaattaat gcaaggtga tcaacctga agcacttcg 
      acaacccgt ggaagtcca aagatggag ggtttcacg 
      aagaccact ctggtggta cttagcgac tgtcatagg 
      cctagctat aatttttat tgttgtaac cacatctgt 
      tgttgtcac acgacaatc aaaaaacca ttagttgta 
      caataaaaa tttgatttt tttttataa attattaat 
      ttatttgag taccaatga aaaatgttt acggtcact 
      gatcaatac catgcgtat gcgcagtaa ttgttatgc 
      aactgacat gtgttgctt aacctcaca tttttttaa 
      ggttaaatc aatttcaaa atgttaaaa aacattaga 
      ttacgccct ttagtcaat ttttacaaa tattcaact 
      caaccggtg gctaaagaa acgcccaat tatgacaat 
      cagatcaaa gaattcgaa cgaatttcg aagttgcaa 
      caaaaagtc agaccaaaa aaaccccag agagcacct 
      tttgtcaag aaccttttt ttgggggtg tttgatacc 
      gaccttttg acctatccg gaaatcaca aatgaggaa 
      ttaaacaat ttaaacaaa gctttacaa ccaattcaa 
      actttcgtc caacaaccg gaaatcaaa gattgcaat 
      aaaatcaca cgagacttt ttgaaaaaa ctgtcaaat 
      gcccaactt ttggggctt caagcggcg caattaaac 
      aacggacga gaactaaca gtaaccgaa atgtgtcgt 
      ttttttgaa gttttatcc gaacttaaa ataagaaat 
      ctcctaata cataacgaa gtcttggca atacaaact 
      ttagtaaaa tttggttcg gatcaattg aaacacaag 
      tatttagaa aaactagtt aagggtgaa gcaacttcg 
      gcgttttgt gttcttgaa tcaaaaagc aaaataggg 
      gaaagaaca ggtgtgaaa aatctagat ggcaaaact 
      tgggtttta aacggtgaa agaaaatca gttttgaac 
      ggcagtaat gccgatttt tacatcgta atggcaaac 
      acacaaaac aacacaagg gacgaaatt accgaaaaa 
      aagttcagc ttttttgta gttgagcgc gattttggg 
      ggaatcact agtacaagt tgtggcggc aatgatttg 
      tctgatgtt atttttgaa aatgttaca gtaccacac 
      gaaaatacc atcggtggt gaaaattgt ggagaagag 
      attttatca acaatttat ccacaatat cgcctcagt 
      accggccct ctatgttca gttttagtc aaaaattta 
      ataaataat gtcaccgaa cattttata agagtttca 
      aaaaaacca aacttgttg tacgaaacc gacgccatt 
      cgtacgaaa ttaggacaa ctaacaacg tcactgtat 
      gccattgac agtatggtg tattttacg tcaagtctt 
      gtagatgat tacgaaaat caagactca gagctagaa 
      acgtcaatt gttaaggtt tttgcttca gagcaagct 
      tttctcaat acgtcagct tgtctcgac ttaatcgcc 
      cctgattca gtttttgat aatcactgg tgtaaacaa 
      ctgcatgcg gaagctttg tctcaagtg attttaaac 
      gattctaat gataattta aaaattttg attgcttta 
      tttggacta cgtcacgct ggggaacaa cttaatgaa 
      acaattaaa aaaattcga aacccgttt tactttagc 
      acttatgca tttaaacgc atgtggaca cggcgccgc 
      attgctgag gataaccca aaattaaat ctatatttg 
      catcattat ttgcaccct tcatctatg cttgcttcg 
      caacaattg gagtattgt gttttaaag cttcaatac 
      gccactgaa acgttacta gcacgtcac ggaccggaa 
      gttttgaac aaacatcaa gatttaaga cgtttagct 
      gacgctatt attgacata tatgcaatg gttgcttgt 
      atcagtcgt tcgtcgcgc tcgtattgt ataggttta 
      caacacgcc gactatgag atggtttta gccaatgct 
      ttcacactt ggagccctt gaacgagtc aaattgaac 
      ttgaacaaa ataatagaa ggtccaatt ggcacaaac 
      gacgagaat tatagaatg ttggcaaag agattgttt 
      gaattaaaa cagtactat ccggtgcac ccattaagc 
      cgcaatttt tagttaaat aaactagta attaagcg