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Gene information
General information
TC001293 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 14023666..14033821
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.

    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.

    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02671

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Left: Right:
    Protein sequence
    • >TC001293 RA
    CDS sequence
    • >TC001293 RA
      atgtcggca acgagcacc cctaggcga aaacccgga 
      tttcccgga tccctcacc gacttgcac aggttccgc 
      aagactgtg cacggacac gacggctcc agcaggatt 
      gttatggtc aggaagacc gaccagagg actttcgca 
      tccattgaa aagggacag gaaccgaaa ttagagacg 
      attacgcgt gaaacgatc gaaacattc cgcgggcaa 
      cggaccgaa tgcaaaatc accacagag aagaaaggc 
      tgtgaagac aagttcccg cctgattta atcgaatca 
      ttacaagta tcgcgaacc ccgaagcga actctttcg 
      aataaaaag aaacgaaat ccagttatg gacttccaa 
      caagagtgc cttgcggct cataacgag taccgaaaa 
      cgtcacgga gtccctcca ttgaaactt gacaaagac 
      atgtgcaaa gttgcccaa cagtgggcg aatcattta 
      gtcagcaga aatatactc caacatagc aataacaag 
      gattttggc gaaaattta ttctgcatg acttcgtca 
      aaccccaat gttacagtg tcagggaaa gagccagtg 
      gatgcctgg tatggcgag atcaaagac catactttt 
      ggtgtggaa ccgactagc ctagcttcg ggacatttc 
      actcaagtt gtgtggaaa aacagtgct aaactaggc 
      gtagcgttt gctaaatca ggtggacgg gttgtggta 
      gttgccaat tatgacccc ccaggaaac tatgtgggg 
      agttttagc aaaaacgta ccacctgtt ggtggtttt 
      tcgccaaat aataacaac aacaacaac gatgactgt 
      tctgaagga ctttcaaaa ctgaccatt agtaacggt 
      accaaaccc agtactacc aacaaagac gtgaaaaat 
      ggtaccgaa ggaaatttc gagcaagac tttatcaat 
      gcccataat gagtacaga aaacggcac ggtgtccct 
      tctttaaaa ctcgacaag aaactgtgc aaatacagc 
      gaagaatgg gcaaaacac ctagcggcc aaaaacatc 
      ctcgaacac cgtcctagt tgtaactac ggcgagaat 
      atcttttgt atgtcgtcg tcagacccg aattttacc 
      atttctggg aatactccc gtggaccat tggtatgaa 
      gaagtgcgt tttcaccca tttgggcgc gaacccaac 
      agtctcaaa tccgggcat ttcacgcaa gttgtgtgg 
      aaatcgagc gagttgctg ggtgttggg gtggctaaa 
      aatagccaa gggagtatt tacgtagta gcgaactac 
      tccccagct ggaaacttt gttgggcat tatgttgaa 
      aatgtccca ccactcact tcaaccaaa accggaact 
      actgataag aaagttgct tctccgcaa ggagtcaac 
      aatgagggg tttgcccag tttgcgcta gatgggttg 
      aaagtgcat aatgagtac aggcggaaa catggagtt 
      ccagacttg aaactcaac aaagagatg tgcgactat 
      gcgcaagaa tgggccgac acttgcgcg aaaagcggg 
      agtatgtgc caccgtgcc aacaacccc tacggggaa 
      aatatcttc tgtgtgtac tcttctgat ctgactcac 
      gtgccctct gcacgtgac gccgttaaa gaatggtac 
      gacgaaatc aaaaaacac actttcgga gtggagagt 
      gtgaatcat ggtactctc catttcacc caaattgtt 
      tggaaaggt tgcaccgat ttgggaatg gcaatggcc 
      agtaacaac aaaggagag acctacgtc gttgcaaat 
      tacaatcct cgaggcaac tacgttggc cagttcgtt 
      gataacgtg cctagacca cgattctga
    mRNA sequence
    • >TC001293 RA
      ttcatccaa attcagcgt tattgtaca acttttaat 
      ttatagcgc attgtgtga cactttgtt taacgttac 
      tgataacag tttaaatat tttgctcgt gccttgaag 
      aaaccacca aggatcccg tctgttaaa agtggtggc 
      aaaaaacta taagactag tgaaaaaat tgactgtga 
      tacagtgcg actcaaatc tttgatttc cacacttca 
      tggttccac aacttcgta aataattcc cgtgagaaa 
      aattattac ccaatttta atacattat caagccggt 
      gttttgtgg tcttgataa cccttatct cacttatct 
      taaaaataa tccaggaag ttttcctga aaaaatcgc 
      gattcctgc agcgcatgc ctgcgtatt cctgcaggg 
      ttagtgaaa catcaaccc tcttcagtt ttcagccgt 
      gctgaaatc tgcaccgtt tcagtgcaa gtgcagtgt 
      gtaaacacg atcaataca agcggaaaa catgtcggc 
      aacgagcac ccctaggcg aaaacccgg atttcccgg 
      atccctcac cgacttgca caggttccg caagactgt 
      gcacggaca cgacggctc cagcaggat tgttatggt 
      caggaagac cgaccagag gactttcgc atccattga 
      aaagggaca ggaaccgaa attagagac gattacgcg 
      tgaaacgat cgaaacatt ccgcgggca acggaccga 
      atgcaaaat caccacaga gaagaaagg ctgtgaaga 
      caagttccc gcctgattt aatcgaatc attacaagt 
      atcgcgaac cccgaagcg aactctttc gaataaaaa 
      gaaacgaaa tccagttat ggacttcca acaagagtg 
      ccttgcggc tcataacga gtaccgaaa acgtcacgg 
      agtccctcc attgaaact tgacaaaga catgtgcaa 
      agttgccca acagtgggc gaatcattt agtcagcag 
      aaatatact ccaacatag caataacaa ggattttgg 
      cgaaaattt attctgcat gacttcgtc aaaccccaa 
      tgttacagt gtcagggaa agagccagt ggatgcctg 
      gtatggcga gatcaaaga ccatacttt tggtgtgga 
      accgactag cctagcttc gggacattt cactcaagt 
      tgtgtggaa aaacagtgc taaactagg cgtagcgtt 
      tgctaaatc aggtggacg ggttgtggt agttgccaa 
      ttatgaccc cccaggaaa ctatgtggg gagttttag 
      caaaaacgt accacctgt tggtggttt ttcgccaaa 
      taataacaa caacaacaa cgatgactg ttctgaagg 
      actttcaaa actgaccat tagtaacgg taccaaacc 
      cagtactac caacaaaga cgtgaaaaa tggtaccga 
      aggaaattt cgagcaaga ctttatcaa tgcccataa 
      tgagtacag aaaacggca cggtgtccc ttctttaaa 
      actcgacaa gaaactgtg caaatacag cgaagaatg 
      ggcaaaaca cctagcggc caaaaacat cctcgaaca 
      ccgtcctag ttgtaacta cggcgagaa tatcttttg 
      tatgtcgtc gtcagaccc gaattttac catttctgg 
      gaatactcc cgtggacca ttggtatga agaagtgcg 
      ttttcaccc atttgggcg cgaacccaa cagtctcaa 
      atccgggca tttcacgca agttgtgtg gaaatcgag 
      cgagttgct gggtgttgg ggtggctaa aaatagcca 
      agggagtat ttacgtagt agcgaacta ctccccagc 
      tggaaactt tgttgggca ttatgttga aaatgtccc 
      accactcac ttcaaccaa aaccggaac tactgataa 
      gaaagttgc ttctccgca aggagtcaa caatgaggg 
      gtttgccca gtttgcgct agatgggtt gaaagtgca 
      taatgagta caggcggaa acatggagt tccagactt 
      gaaactcaa caaagagat gtgcgacta tgcgcaaga 
      atgggccga cacttgcgc gaaaagcgg gagtatgtg 
      ccaccgtgc caacaaccc ctacgggga aaatatctt 
      ctgtgtgta ctcttctga tctgactca cgtgccctc 
      tgcacgtga cgccgttaa agaatggta cgacgaaat 
      caaaaaaca cactttcgg agtggagag tgtgaatca 
      tggtactct ccatttcac ccaaattgt ttggaaagg 
      ttgcaccga tttgggaat ggcaatggc cagtaacaa 
      caaaggaga gacctacgt cgttgcaaa ttacaatcc 
      tcgaggcaa ctacgttgg ccagttcgt tgataacgt 
      gcctagacc acgattctg acctttgaa gaagtaatt 
      ttatacaac attccatta ttaagccaa agaattttg 
      tatttttat ttttattat cattattat ttattagac 
      ggtatggtt ataacaaaa tattcctga taagtatta 
      tattgtatg caatcgata gtgagagtt aagcattca 
      gctcatacc aaagagcgg tacactgta cataaataa 
      tcacttgta acaatcaat gttcttgca aattcggtc 
      cattttttg actgtgatt cttaaagac tgtccaagt 
      tgttgcaac tgtttaatc cgtcagcgt ttcaatacc 
      acgagctta tcactcagt tgctgattt aaattattc 
      ggaaagcgt ttaactaac aatgcgata accactctg 
      tgatgtaga tcaattatc tgttgcttt aaacagaac 
      actttgtta aagttatta gcaaactat ttcgagtag 
      ttaggtatt gtatttgtg aggttcttc cattgttga 
      attttgtaa caccctaag ataatcgtt caattttta 
      tatatgcac agtgttaat taaggaaag tgatgtttt 
      tcaacttct agtacaaat tgttgatgt ttacctaat 
      caatactaa cattattat tactatgac gtgtttgtt 
      catacgggc aataaaaat aaaattgta aagtaaaa

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY