Status
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Gene information
General information
TC032201
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC001180,TC001181
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 12293439..12313396
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032201
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02668

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    ovary size decreased - Number of animals showing the phenotype: 3

    egg chamber not present - Number of animals showing the phenotype: 3

    tropharium size decreased - Number of animals showing the phenotype: 3

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032201 RA
      MHHKTTILV APALQNSSC THPRYSQAS FLSLLGLGD 
      VLQALIVFL LTVGVLAAN VLLIVVINS RRYSKYIHS 
      QPRYLLTSL ACNDLAMGL LVTPFAIVP SLRHCWPYG 
      ELVCQIQAL LRGAISQQS AVILICMAM DRYLCMLHP 
      ARYHKHSSK KGCVAIISM TWIMSVTLF SILVLPRGG 
      YYFNPTGMM ACEPFYTRA SIRILAACG FYFPTTMIL 
      MYCYGSAFH VNKLRLKKS GCSTIASPD DYGGTSIEK 
      LVTQERRLS TTASRTMAA MSLGFIVLV TPWTIQEVV 
      AACTGSRAP AALDFLATW LALSNSFWN PFLYWLLNN 
      NFRRISREL LFSRLFCRK SKEPKSHQQ HCCSNSSSG 
      AFTHTQGCD LEGLSEKYW GEILERTLS SSSLQALQR 
      AYSHPRMER CNGLQEMKV FDTAVSLHI FSCTTMYLP 
      VLLAAFFFS RCSSVALPK YIKLCSGSD PNFDKCGLQ 
      SGKEAIKHL VAGEKSLRL LPLSPLKLP FVQLEDRAD 
      FQLNITDAE IIGLNKAEL IGFHADLDK REVTVVVHL 
      AEILLKGQF HTEGRILIL PIKGDGPGS VKAYGGNYT 
      FTFHYNLVD KNGQKYAKI GKNEFKFTI EKAEFAVES 
      LFGDPTIGR ETNRFLNEN WLEVVKDFE HVIGQTIGS 
      ICNNIASIV FERVPYNEI ILP
    CDS sequence
    • >TC032201 RA
      atgcaccac aaaaccacg attttggtg gcgccggct 
      ctgcaaaac agcagctgt acgcatccg aggtatagc 
      caggcgagc tttttgtcc ttgctgggc cttggggat 
      gtcctgcag gcgctgatc gtcttcctg ctgacggtg 
      ggggtcctc gctgcgaac gtgctactc atcgtcgtg 
      atcaacagc aggagatac tccaagtac attcactcg 
      cagccgcgg tatctgctc acgtccctg gcgtgtaac 
      gacctcgcc atgggtctg ctggtgact ccgttcgcg 
      attgtccca tcgttgagg cactgttgg ccctatgga 
      gagctggtc tgtcagatc caggctctg ctcagaggg 
      gctataagt cagcagtct gcagtgatc ttaatatgc 
      atggccatg gataggtac ctctgtatg ctccatcct 
      gcccgctat cacaagcat tccagcaaa aagggatgt 
      gtcgctatc ataagcatg acgtggata atgtcggtt 
      actttgttc tcgattttg gtgctacct cgtggcggt 
      tactacttt aatcccacg ggaatgatg gcttgcgag 
      ccgttttat acccgtgct tccatcagg atcttggcc 
      gcgtgcgga ttttacttt cccacaacc atgattctg 
      atgtactgc tacgggtcc gctttccat gtcaacaag 
      ctgcggctg aagaaatca ggatgcagc accatcgcc 
      agtcccgac gactacggg gggaccagc atagaaaag 
      ctggtgact caggagaga cgcctcagc accacagcg 
      tccaggacg atggccgcc atgtcctta gggttcatc 
      gtccttgtc actccctgg accattcag gaagtcgtc 
      gccgcctgc actggatcc agagccccc gccgctctg 
      gactttttg gcgacttgg cttgcgtta agcaacagc 
      ttctggaac cctttcctc tactggctc ctgaataat 
      aacttcagg aggatcagc agggagctc ctatttagc 
      agacttttc tgtcgcaaa tcgaaagag cccaagtcg 
      caccagcag cactgctgc agcaacagc agctcgggc 
      gctttcacg cacacccaa ggctgtgat ctcgaaggc 
      ctctccgag aagtactgg ggggagatc ctggagagg 
      actctgtcg tccagctcg ctccaggcc ctccagcgc 
      gcctactcc cacccccgc atggagcgc tgcaacggc 
      ctccaagaa atgaaagtg ttcgatacc gccgtatca 
      cttcacatt ttttcctgc actacaatg tatcttcca 
      gtcctccta gctgcattt ttcttcagt cggtgcagt 
      tccgttgca ctgccaaag tacatcaaa ctgtgcagc 
      ggaagcgac ccaaatttc gacaaatgc ggtcttcag 
      agcggcaaa gaggccatc aagcacctg gtggcgggc 
      gagaagtcg ttacgtctg ctgccttta tcgccgcta 
      aaactgccg tttgtgcaa ttggaggac cgggctgat 
      ttccagttg aacatcacc gatgcggaa attatcgga 
      ctcaacaaa gccgaactc attggattc cacgctgat 
      ttggacaaa cgcgaggtc acggtcgtg gtgcatttg 
      gcggagatt ctgctcaaa gggcagttt cacaccgag 
      gggagaatc ctgattctg ccgatcaag ggagacggc 
      cctggatcg gtcaaagct tacggaggc aattacacc 
      ttcactttc cactacaat ctagtcgat aagaacggg 
      caaaagtac gccaaaatt ggcaaaaac gaatttaaa 
      ttcaccatc gagaaggcc gagtttgcc gttgaaagt 
      ctattcggg gatccaact attggtcgg gagacaaac 
      agatttctt aacgaaaac tggttagag gtcgtgaag 
      gattttgaa cacgttatt ggccagacc attggcagc 
      atctgcaat aatattgcc agcatcgtc ttcgagaga 
      gtcccatac aacgaaatt attttaccc taa
    mRNA sequence
    • >TC032201 RA
      aagttcgga aactgcagt cgttatctg gcggtttgc 
      gcgaatggc ctttttggg tttggctga aattgtacg 
      gcgtgatat cgcgagaac taatttggt tttaatttt 
      atgttaatt atttttgaa gattattat tgcctctcc 
      aatgtgttt tgacttgcg agactctgg gaaaatcga 
      gtgcgagat gcttaaaac gagtggcag tgattacaa 
      gcacaggta gcaataaag tgactcaaa atgcaccac 
      aaaaccacg attttggtg gcgccggct ctgcaaaac 
      agcagctgt acgcatccg aggtatagc caggcgagc 
      tttttgtcc ttgctgggc cttggggat gtcctgcag 
      gcgctgatc gtcttcctg ctgacggtg ggggtcctc 
      gctgcgaac gtgctactc atcgtcgtg atcaacagc 
      aggagatac tccaagtac attcactcg cagccgcgg 
      tatctgctc acgtccctg gcgtgtaac gacctcgcc 
      atgggtctg ctggtgact ccgttcgcg attgtccca 
      tcgttgagg cactgttgg ccctatgga gagctggtc 
      tgtcagatc caggctctg ctcagaggg gctataagt 
      cagcagtct gcagtgatc ttaatatgc atggccatg 
      gataggtac ctctgtatg ctccatcct gcccgctat 
      cacaagcat tccagcaaa aagggatgt gtcgctatc 
      ataagcatg acgtggata atgtcggtt actttgttc 
      tcgattttg gtgctacct cgtggcggt tactacttt 
      aatcccacg ggaatgatg gcttgcgag ccgttttat 
      acccgtgct tccatcagg atcttggcc gcgtgcgga 
      ttttacttt cccacaacc atgattctg atgtactgc 
      tacgggtcc gctttccat gtcaacaag ctgcggctg 
      aagaaatca ggatgcagc accatcgcc agtcccgac 
      gactacggg gggaccagc atagaaaag ctggtgact 
      caggagaga cgcctcagc accacagcg tccaggacg 
      atggccgcc atgtcctta gggttcatc gtccttgtc 
      actccctgg accattcag gaagtcgtc gccgcctgc 
      actggatcc agagccccc gccgctctg gactttttg 
      gcgacttgg cttgcgtta agcaacagc ttctggaac 
      cctttcctc tactggctc ctgaataat aacttcagg 
      aggatcagc agggagctc ctatttagc agacttttc 
      tgtcgcaaa tcgaaagag cccaagtcg caccagcag 
      cactgctgc agcaacagc agctcgggc gctttcacg 
      cacacccaa ggctgtgat ctcgaaggc ctctccgag 
      aagtactgg ggggagatc ctggagagg actctgtcg 
      tccagctcg ctccaggcc ctccagcgc gcctactcc 
      cacccccgc atggagcgc tgcaacggc ctccaagaa 
      atgaaagtg ttcgatacc gccgtatca cttcacatt 
      ttttcctgc actacaatg tatcttcca gtcctccta 
      gctgcattt ttcttcagt cggtgcagt tccgttgca 
      ctgccaaag tacatcaaa ctgtgcagc ggaagcgac 
      ccaaatttc gacaaatgc ggtcttcag agcggcaaa 
      gaggccatc aagcacctg gtggcgggc gagaagtcg 
      ttacgtctg ctgccttta tcgccgcta aaactgccg 
      tttgtgcaa ttggaggac cgggctgat ttccagttg 
      aacatcacc gatgcggaa attatcgga ctcaacaaa 
      gccgaactc attggattc cacgctgat ttggacaaa 
      cgcgaggtc acggtcgtg gtgcatttg gcggagatt 
      ctgctcaaa gggcagttt cacaccgag gggagaatc 
      ctgattctg ccgatcaag ggagacggc cctggatcg 
      gtcaaagct tacggaggc aattacacc ttcactttc 
      cactacaat ctagtcgat aagaacggg caaaagtac 
      gccaaaatt ggcaaaaac gaatttaaa ttcaccatc 
      gagaaggcc gagtttgcc gttgaaagt ctattcggg 
      gatccaact attggtcgg gagacaaac agatttctt 
      aacgaaaac tggttagag gtcgtgaag gattttgaa 
      cacgttatt ggccagacc attggcagc atctgcaat 
      aatattgcc agcatcgtc ttcgagaga gtcccatac 
      aacgaaatt attttaccc taattgtta tcgttgact 
      taatttatt ttgttgtaa gtgataaga aattgtgat 
      ctgtaattt aattttatt gtgtttgta ttgtacgat 
      tttcgaaaa tatactttt ttcaataaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY