Status
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Gene information
General information
TC032197
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC001169
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 12217899..12224728
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032197
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02666

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    previtellogenic egg chamber not present - Number of animals showing the phenotype: 3

    vitellogenic egg chamber shape irregular - Number of animals showing the phenotype: 3

    mature egg stage sequence irregular - Number of animals showing the phenotype: 1

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032197 RA
      MKSYACLLV LSALIYSVL GQENFKCPD DFGFYPHHT 
      SCDKYWKCD NNVAELKTC GNGLAFDAS DPKFLTENC 
      DYIHNVDCG DRTQLEPPI SSPHCERLY GIFADESKC 
      DVFWNCWNG EASRYQCSP GLAYDREAR VCMWADQVP 
      ECKNEEVAG GFTCPAPGE VSNSGSFSR HAHPDDCRK 
      YYICLEGTA REYGCPIGT VFKIGDADG TGNCEDPED 
      VPGCEDYYG DLDLKSIRK SELLAGLQS SGSSRSHQP 
      ATKSKPRPA PASRNAPEP ADNN
    • >TC032197 RB
      MKSYACLLV LSALIYSVL GQENFKCPD DFGFYPHHT 
      SCDKYWKCD NNVAELKTC GNGLAFDAS DPKFLTENC 
      DYIHNVDCG DRTQLEPPI SSPHCERLY GIFADESKC 
      DVFWNCWNG EASRYQCSP GLAYDREAR VCMWADQVP 
      ECKNEEVAG GFTCPAPGE VSNSGSFSR HAHPDDCRK 
      YYICLEGTA REYGCPIGT VFKIGDADG TGNCEDPED 
      VPGCEDYYK DVDLKALKK LGY
    • >TC032197 RC
      MKSYACLLV LSALIYSGY RKCHFQNLI RFFFPVLGQ 
      ENFKCPDDF GFYPHHTSC DKYWKCDNN VAELKTCGN 
      GLAFDASDP KFLTENCDY IHNVDCGDR TQLEPPISS 
      PHCERLYGI FADESKCDV FWNCWNGEA SRYQCSPGL 
      AYDREARVC MWADQVPEC KNEEVAGGF TCPAPGEVS 
      NSGSFSRHA HPDDCRKYY ICLEGTARE YGCPIGTVF 
      KIGDADGTG NCEDPEDVP GCEDYYGDL DLKSIRKSE 
      LLAGLQSSG SSRSHQPAT KSKPRPAPA SRNAPEPAD 
      NN
    CDS sequence
    • >TC032197 RA
      atgaagtcc tacgcgtgt ttattggtg ctcagtgca 
      ctcatctat tctgttttg ggacaggaa aatttcaaa 
      tgtccggac gatttcgga ttctaccca catcacacc 
      tcgtgcgat aaatactgg aagtgcgac aacaacgtc 
      gccgagctc aagacgtgc ggaaatggg cttgctttc 
      gacgccagc gatcccaaa tttttgact gaaaactgt 
      gattacatc cataatgtc gactgcggc gacaggacg 
      cagcttgaa ccgccaatt tcgtcgccg cactgcgaa 
      cggctttac ggaatcttc gccgatgag agcaaatgc 
      gacgtgttc tggaattgc tggaacggc gaagcttcc 
      aggtaccaa tgctcaccc ggactggcc tacgacagg 
      gaagccagg gtctgcatg tgggccgac caggtccca 
      gagtgcaaa aatgaagaa gtagctgga ggcttcacc 
      tgccccgcc cccggcgaa gtgagcaac tcgggctcg 
      ttctcccga cacgcgcac cccgacgac tgccgcaag 
      tactacatc tgcctggag ggcaccgct cgcgagtac 
      ggctgtccc atcggcacc gtcttcaaa atcggcgac 
      gctgacggc accggaaac tgcgaggac ccggaggac 
      gtgcccgga tgcgaggac tactacggc gatttggac 
      ttgaaaagc atccgcaag agcgagctg ttggccggg 
      ctccaaagc agcggatcc tcgagaagc caccagccc 
      gcgacgaag agcaagccc aggccggcc cccgcgtcg 
      cgcaacgcc cccgaaccc gccgataat aattaa
    • >TC032197 RB
      atgaagtcc tacgcgtgt ttattggtg ctcagtgca 
      ctcatctat tctgttttg ggacaggaa aatttcaaa 
      tgtccggac gatttcgga ttctaccca catcacacc 
      tcgtgcgat aaatactgg aagtgcgac aacaacgtc 
      gccgagctc aagacgtgc ggaaatggg cttgctttc 
      gacgccagc gatcccaaa tttttgact gaaaactgt 
      gattacatc cataatgtc gactgcggc gacaggacg 
      cagcttgaa ccgccaatt tcgtcgccg cactgcgaa 
      cggctttac ggaatcttc gccgatgag agcaaatgc 
      gacgtgttc tggaattgc tggaacggc gaagcttcc 
      aggtaccaa tgctcaccc ggactggcc tacgacagg 
      gaagccagg gtctgcatg tgggccgac caggtccca 
      gagtgcaaa aatgaagaa gtagctgga ggcttcacc 
      tgccccgcc cccggcgaa gtgagcaac tcgggctcg 
      ttctcccga cacgcgcac cccgacgac tgccgcaag 
      tactacatc tgcctggag ggcaccgct cgcgagtac 
      ggctgtccc atcggcacc gtcttcaaa atcggcgac 
      gctgacggc accggaaac tgcgaggac ccggaggac 
      gtgcccgga tgtgaagat tactacaaa gatgtagac 
      ttaaaggcc ctgaaaaag ttagggtac tga
    • >TC032197 RC
      atgaagtcc tacgcgtgt ttattggtg ctcagtgca 
      ctcatctat tctggttac cgaaaatgt cactttcaa 
      aacctgatt cgttttttt ttcccagtt ttgggacag 
      gaaaatttc aaatgtccg gacgatttc ggattctac 
      ccacatcac acctcgtgc gataaatac tggaagtgc 
      gacaacaac gtcgccgag ctcaagacg tgcggaaat 
      gggcttgct ttcgacgcc agcgatccc aaatttttg 
      actgaaaac tgtgattac atccataat gtcgactgc 
      ggcgacagg acgcagctt gaaccgcca atttcgtcg 
      ccgcactgc gaacggctt tacggaatc ttcgccgat 
      gagagcaaa tgcgacgtg ttctggaat tgctggaac 
      ggcgaagct tccaggtac caatgctca cccggactg 
      gcctacgac agggaagcc agggtctgc atgtgggcc 
      gaccaggtc ccagagtgc aaaaatgaa gaagtagct 
      ggaggcttc acctgcccc gcccccggc gaagtgagc 
      aactcgggc tcgttctcc cgacacgcg caccccgac 
      gactgccgc aagtactac atctgcctg gagggcacc 
      gctcgcgag tacggctgt cccatcggc accgtcttc 
      aaaatcggc gacgctgac ggcaccgga aactgcgag 
      gacccggag gacgtgccc ggatgcgag gactactac 
      ggcgatttg gacttgaaa agcatccgc aagagcgag 
      ctgttggcc gggctccaa agcagcgga tcctcgaga 
      agccaccag cccgcgacg aagagcaag cccaggccg 
      gcccccgcg tcgcgcaac gcccccgaa cccgccgat 
      aataattaa
    mRNA sequence
    • >TC032197 RA
      ggcctcctg cggaaacac agtgataca tacttaagt 
      gcacaaaga tgaagtcct acgcgtgtt tattggtgc 
      tcagtgcac tcatctatt ctgttttgg gacaggaaa 
      atttcaaat gtccggacg atttcggat tctacccac 
      atcacacct cgtgcgata aatactgga agtgcgaca 
      acaacgtcg ccgagctca agacgtgcg gaaatgggc 
      ttgctttcg acgccagcg atcccaaat ttttgactg 
      aaaactgtg attacatcc ataatgtcg actgcggcg 
      acaggacgc agcttgaac cgccaattt cgtcgccgc 
      actgcgaac ggctttacg gaatcttcg ccgatgaga 
      gcaaatgcg acgtgttct ggaattgct ggaacggcg 
      aagcttcca ggtaccaat gctcacccg gactggcct 
      acgacaggg aagccaggg tctgcatgt gggccgacc 
      aggtcccag agtgcaaaa atgaagaag tagctggag 
      gcttcacct gccccgccc ccggcgaag tgagcaact 
      cgggctcgt tctcccgac acgcgcacc ccgacgact 
      gccgcaagt actacatct gcctggagg gcaccgctc 
      gcgagtacg gctgtccca tcggcaccg tcttcaaaa 
      tcggcgacg ctgacggca ccggaaact gcgaggacc 
      cggaggacg tgcccggat gcgaggact actacggcg 
      atttggact tgaaaagca tccgcaaga gcgagctgt 
      tggccgggc tccaaagca gcggatcct cgagaagcc 
      accagcccg cgacgaaga gcaagccca ggccggccc 
      ccgcgtcgc gcaacgccc ccgaacccg ccgataata 
      attaatcca taaacctca tcacccgta acctcatat 
      ctaatggca gaccgtcat tcggtagac tgagatact 
      cacttttgt attatttaa tttaataga atttaagtt 
      taaggtccg ggtatctgc tttaaccga ctgaccttc 
      tgttcctat attgtagat taatttaaa atggttcaa 
      aactctact tgctaaatt tgttcattc tctatgcca 
      cggtacaaa aatacattt tttataaga aataaaaac 
      tgtttttta ccccccttg ttttttttc aacccccga 
      gcggctata aggacttta cttaacaag gctgaggct 
      aacattttc aatttcacg taacgacct tttggttgg 
      gtgccacct ttacacaag agtcacatt taaccaaaa 
      ccttgggaa aagaaattg tcaaataaa agtactttc 
      ttttggcta aatgtgacc cttaccgca ggcgaaagc 
      ggtgtgtaa aaagcggtg aaacggttt ggtgaagcg 
      tagtaacaa atgtttttt tcctacagt gaagattac 
      tacaaagat gtagactta aaggccctg aaaaagtta 
      gggtactga gatggagtg aagtggcct gtacagaac 
      ttagtttaa gtctcaacg cctccagtg aatgtgtgt 
      gtgtttaag atgagctaa agcagctca aacttcgct 
      gcacgtctt gtcgttgat tttttgttt gaatttgtt 
      tttaatagt actgtatgt tgtaagaat gaataaata 
      aattatgta aggaaca
    • >TC032197 RB
      ggcctcctg cggaaacac agtgataca tacttaagt 
      gcacaaaga tgaagtcct acgcgtgtt tattggtgc 
      tcagtgcac tcatctatt ctgttttgg gacaggaaa 
      atttcaaat gtccggacg atttcggat tctacccac 
      atcacacct cgtgcgata aatactgga agtgcgaca 
      acaacgtcg ccgagctca agacgtgcg gaaatgggc 
      ttgctttcg acgccagcg atcccaaat ttttgactg 
      aaaactgtg attacatcc ataatgtcg actgcggcg 
      acaggacgc agcttgaac cgccaattt cgtcgccgc 
      actgcgaac ggctttacg gaatcttcg ccgatgaga 
      gcaaatgcg acgtgttct ggaattgct ggaacggcg 
      aagcttcca ggtaccaat gctcacccg gactggcct 
      acgacaggg aagccaggg tctgcatgt gggccgacc 
      aggtcccag agtgcaaaa atgaagaag tagctggag 
      gcttcacct gccccgccc ccggcgaag tgagcaact 
      cgggctcgt tctcccgac acgcgcacc ccgacgact 
      gccgcaagt actacatct gcctggagg gcaccgctc 
      gcgagtacg gctgtccca tcggcaccg tcttcaaaa 
      tcggcgacg ctgacggca ccggaaact gcgaggacc 
      cggaggacg tgcccggat gtgaagatt actacaaag 
      atgtagact taaaggccc tgaaaaagt tagggtact 
      gagatggag tgaagtggc ctgtacaga acttagttt 
      aagtctcaa cgcctccag tgaatgtgt gtgtgttta 
      agatgagct aaagcagct caaacttcg ctgcacgtc 
      ttgtcgttg attttttgt ttgaatttg tttttaata 
      gtactgtat gttgtaaga atgaataaa taaattatg 
      taaggaaca caggtttta tttgcggga gcagaaaaa 
      aatcgctgg gatttaaaa tatcttttt aattatgat 
      agtcaggta ggtatattt acatcattc tttaaatta 
      aactataca gtaatatca ggtatggca tctttaatt 
      atttacaaa cacagttgg acaactgtg gtctttaaa 
      aacctgtaa aaaatcata gtgcatgtg aatttcgta 
      attctaata ctacaactt atctagaca tgaatcggt 
      aaagataat taacagcct ttaaaaata ttaaactgt 
      agcagacaa atacatcac ttggacttg cagaatcgc 
      caaatgcac atttttaat cgtttggtt aattatgtc 
      ttaaatgta aaaagagcc cgaatctac tttagggct 
      tgaatctgt gcgaatggc gggaataaa actgttacg 
      aaatgtttt tttggtgcc gttttcgag agaacagat 
      aagacaatg agtcgtcag tccacgttt ggcttccgc 
      acgaacgat ttacggtct tattgattg ttaataatt 
      aaaataccg gtgttttca aaaagtgag tacttaagt 
      gctggtgta tggtgacgc tgtccaggt tcaacaaat 
      gtggtgagt gagatttta tagcaaaac gtttggcat 
      ttggtgggt tctgcaagt ggccaaaat gagtgcatt 
      tatttctgc cacagtcgt aattaaaat ataaattag 
      agcattttt ctaaactga tgttaatta caattaaat 
      aaaataaat attaacata gcagaaaac cgagaaatt 
      gaaacgagt ctcgatatt ttttaatag aggaaatta 
      aaaaacacg aaacttgga ctttatcga catcactcg 
      ataaaacat caaccgtaa gcgagcaat ttgtattgt 
      aataaatat cagtccaat aatatttaa ttttaaaat 
      catttaata ctaaaatta tttatctct aaaatagat 
      cacttaaaa aaataatac acaggacca gcaaccaac 
      atcctgacg gattaaaga ctagtaatg taaagtgac 
      tatgtttta caaacacag cagaacaca cgattataa 
      ctaaaagaa cttgccaat ttcaaagac agtgacgat 
      taacgaatt ttgcgccgc aatatcgct atcacgact 
      gaatttatt taaactaac ccgaacaaa tcattctga 
      agcaccatt taaacaaaa tcacgaagt ttctttatt 
      tatgaaaag ccgtacaac gcaagcgag tccgctgta 
      aaataggtg tgaatcagc tcaatagaa attctccaa 
      atccaaatt taaccacaa tttatcact cttctgaac 
      aacattata cctaaataa tcagctttt ctattaaat 
      acttcatat ttttgtcta ccttcctta taacaacat 
      ttaggcatg gtttgttct aaaataaca taagaaaca 
      acaatttta atc
    • >TC032197 RC
      ggcctcctg cggaaacac agtgataca tacttaagt 
      gcacaaaga tgaagtcct acgcgtgtt tattggtgc 
      tcagtgcac tcatctatt ctggttacc gaaaatgtc 
      actttcaaa acctgattc gtttttttt tcccagttt 
      tgggacagg aaaatttca aatgtccgg acgatttcg 
      gattctacc cacatcaca cctcgtgcg ataaatact 
      ggaagtgcg acaacaacg tcgccgagc tcaagacgt 
      gcggaaatg ggcttgctt tcgacgcca gcgatccca 
      aatttttga ctgaaaact gtgattaca tccataatg 
      tcgactgcg gcgacagga cgcagcttg aaccgccaa 
      tttcgtcgc cgcactgcg aacggcttt acggaatct 
      tcgccgatg agagcaaat gcgacgtgt tctggaatt 
      gctggaacg gcgaagctt ccaggtacc aatgctcac 
      ccggactgg cctacgaca gggaagcca gggtctgca 
      tgtgggccg accaggtcc cagagtgca aaaatgaag 
      aagtagctg gaggcttca cctgccccg cccccggcg 
      aagtgagca actcgggct cgttctccc gacacgcgc 
      accccgacg actgccgca agtactaca tctgcctgg 
      agggcaccg ctcgcgagt acggctgtc ccatcggca 
      ccgtcttca aaatcggcg acgctgacg gcaccggaa 
      actgcgagg acccggagg acgtgcccg gatgcgagg 
      actactacg gcgatttgg acttgaaaa gcatccgca 
      agagcgagc tgttggccg ggctccaaa gcagcggat 
      cctcgagaa gccaccagc ccgcgacga agagcaagc 
      ccaggccgg cccccgcgt cgcgcaacg cccccgaac 
      ccgccgata ataattaat ccataaacc tcatcaccc 
      gtaacctca tatctaatg gcagaccgt cattcggta 
      gactgagat actcacttt tgtattatt taatttaat 
      agaatttaa gtttaaggt ccgggtatc tgctttaac 
      cgactgacc ttctgttcc tatattgta gattaattt 
      aaaatggtt caaaactct acttgctaa atttgttca 
      ttctctatg ccacggtac aaaaataca ttttttata 
      agaaataaa aactgtttt ttacccccc ttgtttttt 
      ttcaacccc cgagcggct ataaggact ttacttaac 
      aaggctgag gctaacatt ttcaatttc acgtaacga 
      ccttttggt tgggtgcca cctttacac aagagtcac 
      atttaacca aaaccttgg gaaaagaaa ttgtcaaat 
      aaaagtact ttcttttgg ctaaatgtg acccttacc 
      gcaggcgaa agcggtgtg taaaaagcg gtgaaacgg 
      tttggtgaa gcgtagtaa caaatgttt ttttcctac 
      agtgaagat tactacaaa gatgtagac ttaaaggcc 
      ctgaaaaag ttagggtac tgagatgga gtgaagtgg 
      cctgtacag aacttagtt taagtctca acgcctcca 
      gtgaatgtg tgtgtgttt aagatgagc taaagcagc 
      tcaaacttc gctgcacgt cttgtcgtt gattttttg 
      tttgaattt gtttttaat agtactgta tgttgtaag 
      aatgaataa ataaattat gtaaggaac a
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