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Gene information
General information
TC032178
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC001131,TC001132
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 5945779..5952236
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032178
    iBeetle screen iB_02663
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032178 RA
      MNQDQEVPT AVSELSRLG CLFGSPFSS TCYQQYQEH 
      YKSTPPAGV FPTDWREPW HQEGNSCET DDSEQYVSD 
      SDFQQQNTS QIKPENNMS DLLNKPPTY PSSYSGNDN 
      FDGVYAPTY QHTGCFYSN PIYNRSTSP VVPRGNEHI 
      TSNLQGIHQ TNLPIKQEP ADRDFEGNF RTCYDKCKS 
      TPASKSHWK QPMGLINPD KTLDEKCRE SADNRSNFQ 
      NASGRRGSL QLWQFLVAL LDAPDASAG CIAWTGRGM 
      EFKLIEPEE VARRWGAQK NRPAMNYDK LSRSLRYYY 
      EKGIMQKVA GERYVYKFV CDPDALFNM AYGGHHENS 
      VRSQIANRP ENLSTKTET HHLAYTDML NFYNCGIAH 
      PYQHLHPYL QDSKHSRYP
    CDS sequence
    • >TC032178 RA
      atgaaccag gaccaagaa gttccaact gcagtcagt 
      gaattaagt agacttggg tgtctgttc ggttccccc 
      ttctcttcg acgtgctac cagcagtat caggagcat 
      tacaagtcc actccaccc gccggcgta ttcccaaca 
      gactggaga gagccctgg catcaagaa gggaacagt 
      tgtgaaact gacgatagt gagcagtat gtctcagat 
      tcggacttt cagcagcag aataccagt cagataaag 
      ccggaaaat aacatgtcg gatctgcta aacaaacct 
      ccaacatat cctagttcg tattcaggg aacgacaat 
      tttgacggg gtgtacgcc cccacctac caacacact 
      ggatgtttt tactcaaat cccatttac aacaggtct 
      acttcacca gtagtaccc agaggtaat gagcacatt 
      acgagtaac ttacaagga attcatcaa actaactta 
      cccataaaa caagaacct gcagacaga gactttgaa 
      ggcaacttt aggacgtgt tacgacaaa tgcaaaagc 
      acgccggcg agtaaaagc cactggaag cagccgatg 
      ggcctcatc aacccagat aaaactctg gacgagaag 
      tgtcgcgaa tctgccgat aatcgatcc aacttccag 
      aatgcaagt ggcagaaga gggtcactg caattgtgg 
      cagtttttg gtggcactt ttggacgcg ccagacgcc 
      agtgctggc tgcatagcc tggacaggc cgagggatg 
      gagtttaaa ttgatcgag cccgaagag gtggcccgg 
      agatggggc gctcagaaa aaccggcca gcgatgaat 
      tacgacaaa cttagccga tcgctgaga tactactac 
      gaaaagggc ataatgcag aaagttgca ggggagaga 
      tatgtttac aaatttgtt tgtgatcct gatgctttg 
      ttcaatatg gcttatgga gggcaccat gaaaattca 
      gtgcggtcg caaatcgca aatcggccg gaaaatctt 
      agtacgaaa actgaaact catcatctg gcttacaca 
      gatatgctc aatttctat aattgtgga attgcccat 
      ccctaccaa cacctgcat ccgtatttg caagattcg 
      aagcattcg cgttatccg tga
    mRNA sequence
    • >TC032178 RA
      gtcgagagg gatgaacca ggaccaaga agttccaac 
      tgcagtcag tgaattaag tagacttgg gtgtctgtt 
      cggttcccc cttctcttc gacgtgcta ccagcagta 
      tcaggagca ttacaagtc cactccacc cgccggcgt 
      attcccaac agactggag agagccctg gcatcaaga 
      agggaacag ttgtgaaac tgacgatag tgagcagta 
      tgtctcaga ttcggactt tcagcagca gaataccag 
      tcagataaa gccggaaaa taacatgtc ggatctgct 
      aaacaaacc tccaacata tcctagttc gtattcagg 
      gaacgacaa ttttgacgg ggtgtacgc ccccaccta 
      ccaacacac tggatgttt ttactcaaa tcccattta 
      caacaggtc tacttcacc agtagtacc cagaggtaa 
      tgagcacat tacgagtaa cttacaagg aattcatca 
      aactaactt acccataaa acaagaacc tgcagacag 
      agactttga aggcaactt taggacgtg ttacgacaa 
      atgcaaaag cacgccggc gagtaaaag ccactggaa 
      gcagccgat gggcctcat caacccaga taaaactct 
      ggacgagaa gtgtcgcga atctgccga taatcgatc 
      caacttcca gaatgcaag tggcagaag agggtcact 
      gcaattgtg gcagttttt ggtggcact tttggacgc 
      gccagacgc cagtgctgg ctgcatagc ctggacagg 
      ccgagggat ggagtttaa attgatcga gcccgaaga 
      ggtggcccg gagatgggg cgctcagaa aaaccggcc 
      agcgatgaa ttacgacaa acttagccg atcgctgag 
      atactacta cgaaaaggg cataatgca gaaagttgc 
      aggggagag atatgttta caaatttgt ttgtgatcc 
      tgatgcttt gttcaatat ggcttatgg agggcacca 
      tgaaaattc agtgcggtc gcaaatcgc aaatcggcc 
      ggaaaatct tagtacgaa aactgaaac tcatcatct 
      ggcttacac agatatgct caatttcta taattgtgg 
      aattgccca tccctacca acacctgca tccgtattt 
      gcaagattc gaagcattc gcgttatcc gtgagatga 
      tttggtgcg tttgatttt ggcgaaatt tcttctttt 
      atcttgatg ctagtcagt tgtaagtca ttcattttg 
      tgtaaatta ttataaata cgtaaatgt tgtaacaaa 
      taaatagaa catgtaatg