General information | |||
TC032177
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 11433972..11449200 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
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TC032177 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_02662 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
remarks: muscle pattern potentially obscured by segmentation defect,
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): less than 30%
cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC032177 RA
MTCNYNVKA ISNRNYNIH GGLINNNYT QVALPTRIN NSARKLVKI SKVQCESLT CFDKSIKEE KIRLAGTEL TVESGGDKS CLTTYIFNP VDTCTGSPS PEKFPLKTP DQQRDDVIN TETHMHPNF LYGKILHDG RPNFLTEYE LSYKNCNFG VADHGKCCV GGHFGRDLS FGHLYTISK SDKEERTYD EPYTGDLPR RSWLCCPGS QHHVECEQP PPLQTNKSP SSPCEAITK APSHNLCPG NCERLQQQL DEQAQRYEE QLTELHSVI AELTRKLHQ QRTMAIAEE DEVSESCAS AHDDSVTCP LEVSELEPL ENELSDLDN KVVLSESPL ELPEPKLSS SHHENNELS PYSEIVDPL KQEIMHLRA QLQEANAKL LQISSTQKN YEIAEEETL TDSKVLESP ESHIEVDCD PKLYSDDKV DIKYSNIVP IHRANVKNN TAFSTAPVT KVAERIKLK RAADGHRDV NPNDLVNSD LPTAVAEHI VGDILRQCD VQTEKQAID IELRRLNAK LEHARAQNS VLAITLTET KAHCDRLAL LCGKYESNA IALRLALGI TDRAIEAYD VLLALLETE LSLDENDST SVENRSAAE TVAKQLLTH LDSYQNTDV LLSPWQNHV YSSPVSENE DPWSSEHET RLREHVSRL KAERSNIQG TYVTLESTQ VENVPVRPS SSQEARKMD LEMAVLMQE LMGLREDKA ELISKLFML EKDKSAIEL KLKYVEGQQ KAQAAALKN LQGQLKDTE ALLALATQN KERGYSDAE HAQGVELEL MQALAREAR LKVRLQELV TTLEQVTKN AEARLLEGR EIVHDLNHT NSILAETVD RNNKKYQSK FKKMEHQML AMVEKHNLQ VQNLQQRIA TLETPPTNS SDNSINSVA V
- >TC032177 RA
atgacatgt aattacaac gttaaagca ataagtaat agaaattat aatatacac ggaggatta ataaataat aattacaca caagttgca ttaccaaca agaatcaat aactccgct agaaaactg gtcaaaatc agcaaagtt cagtgtgag agtctgaca tgtttcgat aagagcatc aaagaggaa aaaatcagg ctggcgggc acagaactc accgttgaa agtggaggc gacaaaagc tgcctcacc acttacata ttcaacccc gttgacact tgcacgggc tctcccagt cccgaaaaa ttcccactg aagactccc gaccagcag cgggatgat gtcatcaac acggagacc cacatgcac ccaaacttc ctctacgga aaaatcctc cacgacggc cggcctaac tttctaacc gaatatgag ctgagctac aaaaactgc aattttggg gtggctgac cacggcaag tgctgcgtg gggggccat ttcgggaga gacttgagt tttggacac ttgtacact atcagcaag agcgacaag gaggagagg acgtacgat gagccctac acgggggac ttgccgcgg aggtcctgg ctgtgctgt ccaggctcg cagcatcat gtggaatgc gagcaaccg ccccctttg caaaccaac aagtcaccg tccagccct tgcgaagcg attacaaaa gcgcccagc cacaatctg tgtcctgga aattgcgag agactccag cagcaattg gacgaacaa gcacagcga tacgaagaa cagctgaca gagctgcac tcggttata gctgaattg acgcgcaaa ttgcaccaa cagagaact atggcaatt gcagaagaa gacgaagtg tcagaatct tgtgcaagc gcccatgac gattccgtg acgtgtcct ttggaagta agcgaatta gaaccgtta gaaaacgag ttgtccgac ttggacaat aaagtagta ttgtccgaa agtccatta gaactcccc gagccgaaa ctaagctcg tcgcatcac gagaacaac gaactttca ccatactca gaaatcgta gatccatta aaacaggaa ataatgcat ctcagggcc caactgcaa gaagccaac gccaaactg ttacagatc tcctcgaca cagaagaat tacgaaatc gccgaagaa gaaactctg accgatagc aaagttctg gaatcgccg gaatctcat attgaagtt gactgcgac ccgaaattg tattccgat gataaagtc gacataaaa tacagcaat attgttcct attcatcga gccaacgtc aaaaataac accgccttc tcaactgct ccggtcact aaagttgca gagaggatc aagttgaaa cgggccgcc gatggccac agagacgtt aatcctaat gatctggtt aacagtgat ctgccgaca gctgtcgca gagcacatt gttggcgat attttaaga cagtgcgat gtacaaacc gaaaaacaa gcaattgat atcgaacta aggcgctta aatgccaaa ctcgaacat gccagggcg caaaactcc gtcctggcc atcaccttg accgaaact aaagcccat tgcgacaga ttggcctta ctttgcgga aaatatgaa tccaacgca atagcttta cgcctggcc ttgggaatc accgatcgt gcaatagaa gcctacgac gtcctttta gctttacta gagactgaa ttatcgtta gacgaaaac gattcaacg tctgttgaa aatagatca gccgccgaa accgtcgct aaacagctc ctaactcat ttggattca tatcaaaac accgacgtt ctgttatct ccgtggcaa aaccacgtt tactctagt ccagtatcg gaaaatgag gacccctgg agttcggaa catgaaact agactaaga gagcacgtt tcgaggctt aaagccgaa agaagcaat attcaagga acttacgtg actttggag tccactcaa gtggaaaat gtacctgtc agaccatca agtagtcaa gaagcgaga aaaatggat ttggaaatg gctgttttg atgcaggag ctaatggga ctcagggaa gacaaagcc gaacttata tcgaagcta tttatgtta gaaaaagac aaaagtgcc atagagtta aagttgaag tacgtcgaa gggcagcag aaagcccaa gcggcggcg cttaaaaac ctccaagga cagctgaaa gacacggaa gctttattg gccttagcg acacagaat aaagagagg ggttattcg gatgcagag catgctcaa ggtgttgaa ctcgagcta atgcaagcg ctggcaaga gaggcaaga ctgaaagtt cgcttacaa gaacttgtc acgactttg gaacaagtg acaaaaaat gccgaagcg agactattg gaagggaga gagatcgtg cacgatctg aatcacaca aatagtatt ttagccgaa actgtagat agaaacaat aagaagtat caatccaag tttaagaaa atggagcat cagatgtta gcaatggta gaaaagcac aaccttcaa gtccagaat ctccaacag agaatagcc acccttgaa actcccccc accaatagt tcagacaac tctatcaat tctgtagct gtgtag
- >TC032177 RA
gcggtcgga tagcagtgg aaacacgcg cgtgccgcc gccggtcgg tctttggtg acagtgcgt ttcttgaca tacatccat gtgctgaca catcttgag tgtcatctg ctgtacatc caacgagga aaacgacgg aatagcgta aaaaacgca ccgttttta cagttctga agaggatct tgtgaatcc caaattgta ggaattagt gctttgata atagattgt tgacttgtt ggtgacgca atttcgctg gttcgaatt tgtttaagt ccttatcga gtagggttg aaacgaatt tgaataatt taacgccaa aggtgtgaa tgtgctaaa ttggtgttt ttacagctt cattcaaaa tgaagtaat tgttgattt tggagttgt cacaaacca caaacgcgt aacaatcga ttgagtcta ataagtatt caaacaact tggtggttt taactttta aacaattca caacatcac gaaatgtgt tttaatgtc agttccaaa cttgtttcg gtgttatta cttcgagta tctaaagca tatacgtgt aattccaat tcggggttt ctcctggga ttgtgttta tttaaggga tatcagtaa cttgggcgc aacagttgc cgatttcca tataaactt gccctaaag gtgagtctg gcgtttgaa ttatttcgt gtgcgtcag cagtgacta aaacggggt aatatatga ccgtcgttt cctacaaag ttggtacac ttggctgcg tcaccgaaa ggtgtgctt ttttgaatg aacacattg tagggtaag aaatggaag cactttaca catttcgtc gtattatgt tttcgctcg tttcgctcc cattttcga gtaatattg aaagaagat gctgttgta tcgtatgta tacgatagg caaataaag ctcgaaaca acaaacatt gtctaaaac tatttcgag accatccga tgaagtgcc tcactaaaa gagaaagtg gatgtaaac tacactaat ttcgttaat gtgctttcg gcttggatt tcattacaa aattggtga cgtttataa tcttagttt ttgtgttaa tcaaatttt aattaaatt cggtacaag tagtggcgt acttacttg tcaaggcgc ttaaaacca caacgaaat tagtgatca gtcgggctc aattaaatc aagaaaaga ctaaagtga ctttatggt ttcagagaa aaaacgcat tacattttc gtcgcgtgc ttttacctt aattaaatt acaaaaata aagcactgc ttgaagtac cctacgcat gattaattt taaaataga atgactgga aatattctt cggttggcg ttaaattgc tgagaaaat attttcact gctattaat aaccagaaa tataaacta ggaaacgat gcatagttt ctactaaaa agtcaggct ttttatttc tttttcggc gttttaatt gctcaacct gtttgatta ttaattgac ctatcgtct tttgtttta attaaaagg taagagacg aaatgttca cataagttg acataaata tttcgtttc gaaagaaag cattgtatt aacagcact tggacagcc acaaccgac caaattagc tttattgat gggtctatg tgatatcca aaaagccac caagtctcg gtgcatgta ctgaattct aatagcgct acggctttg ttttaccat attcataag tgcacatta actgtaata tggactgga actggacgt gataataat tgcaataat taaccgtta ggtatttca agcaattct tgtaatgac atgtaatta caacgttaa agcaataag taatagaaa ttataatat acacggagg attaataaa taataatta cacacaagt tgcattacc aacaagaat caataactc cgctagaaa actggtcaa aatcagcaa agttcagtg tgagagtct gacatgttt cgataagag catcaaaga ggaaaaaat caggctggc gggcacaga actcaccgt tgaaagtgg aggcgacaa aagctgcct caccactta catattcaa ccccgttga cacttgcac gggctctcc cagtcccga aaaattccc actgaagac tcccgacca gcagcggga tgatgtcat caacacgga gacccacat gcacccaaa cttcctcta cggaaaaat cctccacga cggccggcc taactttct aaccgaata tgagctgag ctacaaaaa ctgcaattt tggggtggc tgaccacgg caagtgctg cgtgggggg ccatttcgg gagagactt gagttttgg acacttgta cactatcag caagagcga caaggagga gaggacgta cgatgagcc ctacacggg ggacttgcc gcggaggtc ctggctgtg ctgtccagg ctcgcagca tcatgtgga atgcgagca accgccccc tttgcaaac caacaagtc accgtccag cccttgcga agcgattac aaaagcgcc cagccacaa tctgtgtcc tggaaattg cgagagact ccagcagca attggacga acaagcaca gcgatacga agaacagct gacagagct gcactcggt tatagctga attgacgcg caaattgca ccaacagag aactatggc aattgcaga agaagacga agtgtcaga atcttgtgc aagcgccca tgacgattc cgtgacgtg tcctttgga agtaagcga attagaacc gttagaaaa cgagttgtc cgacttgga caataaagt agtattgtc cgaaagtcc attagaact ccccgagcc gaaactaag ctcgtcgca tcacgagaa caacgaact ttcaccata ctcagaaat cgtagatcc attaaaaca ggaaataat gcatctcag ggcccaact gcaagaagc caacgccaa actgttaca gatctcctc gacacagaa gaattacga aatcgccga agaagaaac tctgaccga tagcaaagt tctggaatc gccggaatc tcatattga agttgactg cgacccgaa attgtattc cgatgataa agtcgacat aaaatacag caatattgt tcctattca tcgagccaa cgtcaaaaa taacaccgc cttctcaac tgctccggt cactaaagt tgcagagag gatcaagtt gaaacgggc cgccgatgg ccacagaga cgttaatcc taatgatct ggttaacag tgatctgcc gacagctgt cgcagagca cattgttgg cgatatttt aagacagtg cgatgtaca aaccgaaaa acaagcaat tgatatcga actaaggcg cttaaatgc caaactcga acatgccag ggcgcaaaa ctccgtcct ggccatcac cttgaccga aactaaagc ccattgcga cagattggc cttactttg cggaaaata tgaatccaa cgcaatagc tttacgcct ggccttggg aatcaccga tcgtgcaat agaagccta cgacgtcct tttagcttt actagagac tgaattatc gttagacga aaacgattc aacgtctgt tgaaaatag atcagccgc cgaaaccgt cgctaaaca gctcctaac tcatttgga ttcatatca aaacaccga cgttctgtt atctccgtg gcaaaacca cgtttactc tagtccagt atcggaaaa tgaggaccc ctggagttc ggaacatga aactagact aagagagca cgtttcgag gcttaaagc cgaaagaag caatattca aggaactta cgtgacttt ggagtccac tcaagtgga aaatgtacc tgtcagacc atcaagtag tcaagaagc gagaaaaat ggatttgga aatggctgt tttgatgca ggagctaat gggactcag ggaagacaa agccgaact tatatcgaa gctatttat gttagaaaa agacaaaag tgccataga gttaaagtt gaagtacgt cgaagggca gcagaaagc ccaagcggc ggcgcttaa aaacctcca aggacagct gaaagacac ggaagcttt attggcctt agcgacaca gaataaaga gaggggtta ttcggatgc agagcatgc tcaaggtgt tgaactcga gctaatgca agcgctggc aagagaggc aagactgaa agttcgctt acaagaact tgtcacgac tttggaaca agtgacaaa aaatgccga agcgagact attggaagg gagagagat cgtgcacga tctgaatca cacaaatag tattttagc cgaaactgt agatagaaa caataagaa gtatcaatc caagtttaa gaaaatgga gcatcagat gttagcaat ggtagaaaa gcacaacct tcaagtcca gaatctcca acagagaat agccaccct tgaaactcc ccccaccaa tagttcaga caactctat caattctgt agctgtgta gtaaagtgt gttttaaat gaaattatt tttttattt ttcgttttg ttgatctct gttttaatc agtggcgaa ttgagaaat aaatcaatt tatttttgc cgaacctac tgactgcgc caattaata agcaaggcc aggttttat tacaacaat tacacccaa aatttaaaa aatacccca tcaattttt aatcttatc ttcgggcgt ttttggaga agtaatgtc gggttaatt cgccgctga ttttattat tgatgcatt taatgcggc ctgtatgta taccactaa attgtcgag gaagtatta tgagcagta taagatatt aaagttgta tatgcctag ctgtgatta tttatacat tgctgaatc ggaagtgcg actgattta tatggaaat gttactgca atggttgta ataatgtca ggcagtcaa gtgcttaag cttaattaa atattgttg tttattata aataatatc ggaatagtt tcccagatt tagtacaaa tttattata ttatactaa cgtcttgcc ataacgatg ttttttatg ataacatga ttgagagct gcaagtcaa tcatatcat tcattggag cgtgctgag aatgaagtc ataaattaa catctaaac cgatttcag gaaaattca tttactagt tctaatttt aacacattt taccacgtg ctaaaattc tattttact tacctttga aacgtaatg ggctaataa aacttattt ttgtaaatg aattttcca gtcattatt ttattaaaa cggaataac tataacata cattgattt gtattcagc tatgtattt attagttat tgaaataaa tatgtttac aatat
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
