Status
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Gene information
General information
TC032177
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC030710 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 11433972..11449200
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC032177
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02662

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032177 RA
      MTCNYNVKA ISNRNYNIH GGLINNNYT QVALPTRIN 
NSARKLVKI SKVQCESLT CFDKSIKEE KIRLAGTEL 
TVESGGDKS CLTTYIFNP VDTCTGSPS PEKFPLKTP 
DQQRDDVIN TETHMHPNF LYGKILHDG RPNFLTEYE 
LSYKNCNFG VADHGKCCV GGHFGRDLS FGHLYTISK 
SDKEERTYD EPYTGDLPR RSWLCCPGS QHHVECEQP 
PPLQTNKSP SSPCEAITK APSHNLCPG NCERLQQQL 
DEQAQRYEE QLTELHSVI AELTRKLHQ QRTMAIAEE 
DEVSESCAS AHDDSVTCP LEVSELEPL ENELSDLDN 
KVVLSESPL ELPEPKLSS SHHENNELS PYSEIVDPL 
KQEIMHLRA QLQEANAKL LQISSTQKN YEIAEEETL 
TDSKVLESP ESHIEVDCD PKLYSDDKV DIKYSNIVP 
IHRANVKNN TAFSTAPVT KVAERIKLK RAADGHRDV 
NPNDLVNSD LPTAVAEHI VGDILRQCD VQTEKQAID 
IELRRLNAK LEHARAQNS VLAITLTET KAHCDRLAL 
LCGKYESNA IALRLALGI TDRAIEAYD VLLALLETE 
LSLDENDST SVENRSAAE TVAKQLLTH LDSYQNTDV 
LLSPWQNHV YSSPVSENE DPWSSEHET RLREHVSRL 
KAERSNIQG TYVTLESTQ VENVPVRPS SSQEARKMD 
LEMAVLMQE LMGLREDKA ELISKLFML EKDKSAIEL 
KLKYVEGQQ KAQAAALKN LQGQLKDTE ALLALATQN 
KERGYSDAE HAQGVELEL MQALAREAR LKVRLQELV 
TTLEQVTKN AEARLLEGR EIVHDLNHT NSILAETVD 
RNNKKYQSK FKKMEHQML AMVEKHNLQ VQNLQQRIA 
TLETPPTNS SDNSINSVA V
    CDS sequence
    • >TC032177 RA
      atgacatgt aattacaac gttaaagca ataagtaat 
agaaattat aatatacac ggaggatta ataaataat 
aattacaca caagttgca ttaccaaca agaatcaat 
aactccgct agaaaactg gtcaaaatc agcaaagtt 
cagtgtgag agtctgaca tgtttcgat aagagcatc 
aaagaggaa aaaatcagg ctggcgggc acagaactc 
accgttgaa agtggaggc gacaaaagc tgcctcacc 
acttacata ttcaacccc gttgacact tgcacgggc 
tctcccagt cccgaaaaa ttcccactg aagactccc 
gaccagcag cgggatgat gtcatcaac acggagacc 
cacatgcac ccaaacttc ctctacgga aaaatcctc 
cacgacggc cggcctaac tttctaacc gaatatgag 
ctgagctac aaaaactgc aattttggg gtggctgac 
cacggcaag tgctgcgtg gggggccat ttcgggaga 
gacttgagt tttggacac ttgtacact atcagcaag 
agcgacaag gaggagagg acgtacgat gagccctac 
acgggggac ttgccgcgg aggtcctgg ctgtgctgt 
ccaggctcg cagcatcat gtggaatgc gagcaaccg 
ccccctttg caaaccaac aagtcaccg tccagccct 
tgcgaagcg attacaaaa gcgcccagc cacaatctg 
tgtcctgga aattgcgag agactccag cagcaattg 
gacgaacaa gcacagcga tacgaagaa cagctgaca 
gagctgcac tcggttata gctgaattg acgcgcaaa 
ttgcaccaa cagagaact atggcaatt gcagaagaa 
gacgaagtg tcagaatct tgtgcaagc gcccatgac 
gattccgtg acgtgtcct ttggaagta agcgaatta 
gaaccgtta gaaaacgag ttgtccgac ttggacaat 
aaagtagta ttgtccgaa agtccatta gaactcccc 
gagccgaaa ctaagctcg tcgcatcac gagaacaac 
gaactttca ccatactca gaaatcgta gatccatta 
aaacaggaa ataatgcat ctcagggcc caactgcaa 
gaagccaac gccaaactg ttacagatc tcctcgaca 
cagaagaat tacgaaatc gccgaagaa gaaactctg 
accgatagc aaagttctg gaatcgccg gaatctcat 
attgaagtt gactgcgac ccgaaattg tattccgat 
gataaagtc gacataaaa tacagcaat attgttcct 
attcatcga gccaacgtc aaaaataac accgccttc 
tcaactgct ccggtcact aaagttgca gagaggatc 
aagttgaaa cgggccgcc gatggccac agagacgtt 
aatcctaat gatctggtt aacagtgat ctgccgaca 
gctgtcgca gagcacatt gttggcgat attttaaga 
cagtgcgat gtacaaacc gaaaaacaa gcaattgat 
atcgaacta aggcgctta aatgccaaa ctcgaacat 
gccagggcg caaaactcc gtcctggcc atcaccttg 
accgaaact aaagcccat tgcgacaga ttggcctta 
ctttgcgga aaatatgaa tccaacgca atagcttta 
cgcctggcc ttgggaatc accgatcgt gcaatagaa 
gcctacgac gtcctttta gctttacta gagactgaa 
ttatcgtta gacgaaaac gattcaacg tctgttgaa 
aatagatca gccgccgaa accgtcgct aaacagctc 
ctaactcat ttggattca tatcaaaac accgacgtt 
ctgttatct ccgtggcaa aaccacgtt tactctagt 
ccagtatcg gaaaatgag gacccctgg agttcggaa 
catgaaact agactaaga gagcacgtt tcgaggctt 
aaagccgaa agaagcaat attcaagga acttacgtg 
actttggag tccactcaa gtggaaaat gtacctgtc 
agaccatca agtagtcaa gaagcgaga aaaatggat 
ttggaaatg gctgttttg atgcaggag ctaatggga 
ctcagggaa gacaaagcc gaacttata tcgaagcta 
tttatgtta gaaaaagac aaaagtgcc atagagtta 
aagttgaag tacgtcgaa gggcagcag aaagcccaa 
gcggcggcg cttaaaaac ctccaagga cagctgaaa 
gacacggaa gctttattg gccttagcg acacagaat 
aaagagagg ggttattcg gatgcagag catgctcaa 
ggtgttgaa ctcgagcta atgcaagcg ctggcaaga 
gaggcaaga ctgaaagtt cgcttacaa gaacttgtc 
acgactttg gaacaagtg acaaaaaat gccgaagcg 
agactattg gaagggaga gagatcgtg cacgatctg 
aatcacaca aatagtatt ttagccgaa actgtagat 
agaaacaat aagaagtat caatccaag tttaagaaa 
atggagcat cagatgtta gcaatggta gaaaagcac 
aaccttcaa gtccagaat ctccaacag agaatagcc 
acccttgaa actcccccc accaatagt tcagacaac 
tctatcaat tctgtagct gtgtag
    mRNA sequence
    • >TC032177 RA
      gcggtcgga tagcagtgg aaacacgcg cgtgccgcc 
gccggtcgg tctttggtg acagtgcgt ttcttgaca 
tacatccat gtgctgaca catcttgag tgtcatctg 
ctgtacatc caacgagga aaacgacgg aatagcgta 
aaaaacgca ccgttttta cagttctga agaggatct 
tgtgaatcc caaattgta ggaattagt gctttgata 
atagattgt tgacttgtt ggtgacgca atttcgctg 
gttcgaatt tgtttaagt ccttatcga gtagggttg 
aaacgaatt tgaataatt taacgccaa aggtgtgaa 
tgtgctaaa ttggtgttt ttacagctt cattcaaaa 
tgaagtaat tgttgattt tggagttgt cacaaacca 
caaacgcgt aacaatcga ttgagtcta ataagtatt 
caaacaact tggtggttt taactttta aacaattca 
caacatcac gaaatgtgt tttaatgtc agttccaaa 
cttgtttcg gtgttatta cttcgagta tctaaagca 
tatacgtgt aattccaat tcggggttt ctcctggga 
ttgtgttta tttaaggga tatcagtaa cttgggcgc 
aacagttgc cgatttcca tataaactt gccctaaag 
gtgagtctg gcgtttgaa ttatttcgt gtgcgtcag 
cagtgacta aaacggggt aatatatga ccgtcgttt 
cctacaaag ttggtacac ttggctgcg tcaccgaaa 
ggtgtgctt ttttgaatg aacacattg tagggtaag 
aaatggaag cactttaca catttcgtc gtattatgt 
tttcgctcg tttcgctcc cattttcga gtaatattg 
aaagaagat gctgttgta tcgtatgta tacgatagg 
caaataaag ctcgaaaca acaaacatt gtctaaaac 
tatttcgag accatccga tgaagtgcc tcactaaaa 
gagaaagtg gatgtaaac tacactaat ttcgttaat 
gtgctttcg gcttggatt tcattacaa aattggtga 
cgtttataa tcttagttt ttgtgttaa tcaaatttt 
aattaaatt cggtacaag tagtggcgt acttacttg 
tcaaggcgc ttaaaacca caacgaaat tagtgatca 
gtcgggctc aattaaatc aagaaaaga ctaaagtga 
ctttatggt ttcagagaa aaaacgcat tacattttc 
gtcgcgtgc ttttacctt aattaaatt acaaaaata 
aagcactgc ttgaagtac cctacgcat gattaattt 
taaaataga atgactgga aatattctt cggttggcg 
ttaaattgc tgagaaaat attttcact gctattaat 
aaccagaaa tataaacta ggaaacgat gcatagttt 
ctactaaaa agtcaggct ttttatttc tttttcggc 
gttttaatt gctcaacct gtttgatta ttaattgac 
ctatcgtct tttgtttta attaaaagg taagagacg 
aaatgttca cataagttg acataaata tttcgtttc 
gaaagaaag cattgtatt aacagcact tggacagcc 
acaaccgac caaattagc tttattgat gggtctatg 
tgatatcca aaaagccac caagtctcg gtgcatgta 
ctgaattct aatagcgct acggctttg ttttaccat 
attcataag tgcacatta actgtaata tggactgga 
actggacgt gataataat tgcaataat taaccgtta 
ggtatttca agcaattct tgtaatgac atgtaatta 
caacgttaa agcaataag taatagaaa ttataatat 
acacggagg attaataaa taataatta cacacaagt 
tgcattacc aacaagaat caataactc cgctagaaa 
actggtcaa aatcagcaa agttcagtg tgagagtct 
gacatgttt cgataagag catcaaaga ggaaaaaat 
caggctggc gggcacaga actcaccgt tgaaagtgg 
aggcgacaa aagctgcct caccactta catattcaa 
ccccgttga cacttgcac gggctctcc cagtcccga 
aaaattccc actgaagac tcccgacca gcagcggga 
tgatgtcat caacacgga gacccacat gcacccaaa 
cttcctcta cggaaaaat cctccacga cggccggcc 
taactttct aaccgaata tgagctgag ctacaaaaa 
ctgcaattt tggggtggc tgaccacgg caagtgctg 
cgtgggggg ccatttcgg gagagactt gagttttgg 
acacttgta cactatcag caagagcga caaggagga 
gaggacgta cgatgagcc ctacacggg ggacttgcc 
gcggaggtc ctggctgtg ctgtccagg ctcgcagca 
tcatgtgga atgcgagca accgccccc tttgcaaac 
caacaagtc accgtccag cccttgcga agcgattac 
aaaagcgcc cagccacaa tctgtgtcc tggaaattg 
cgagagact ccagcagca attggacga acaagcaca 
gcgatacga agaacagct gacagagct gcactcggt 
tatagctga attgacgcg caaattgca ccaacagag 
aactatggc aattgcaga agaagacga agtgtcaga 
atcttgtgc aagcgccca tgacgattc cgtgacgtg 
tcctttgga agtaagcga attagaacc gttagaaaa 
cgagttgtc cgacttgga caataaagt agtattgtc 
cgaaagtcc attagaact ccccgagcc gaaactaag 
ctcgtcgca tcacgagaa caacgaact ttcaccata 
ctcagaaat cgtagatcc attaaaaca ggaaataat 
gcatctcag ggcccaact gcaagaagc caacgccaa 
actgttaca gatctcctc gacacagaa gaattacga 
aatcgccga agaagaaac tctgaccga tagcaaagt 
tctggaatc gccggaatc tcatattga agttgactg 
cgacccgaa attgtattc cgatgataa agtcgacat 
aaaatacag caatattgt tcctattca tcgagccaa 
cgtcaaaaa taacaccgc cttctcaac tgctccggt 
cactaaagt tgcagagag gatcaagtt gaaacgggc 
cgccgatgg ccacagaga cgttaatcc taatgatct 
ggttaacag tgatctgcc gacagctgt cgcagagca 
cattgttgg cgatatttt aagacagtg cgatgtaca 
aaccgaaaa acaagcaat tgatatcga actaaggcg 
cttaaatgc caaactcga acatgccag ggcgcaaaa 
ctccgtcct ggccatcac cttgaccga aactaaagc 
ccattgcga cagattggc cttactttg cggaaaata 
tgaatccaa cgcaatagc tttacgcct ggccttggg 
aatcaccga tcgtgcaat agaagccta cgacgtcct 
tttagcttt actagagac tgaattatc gttagacga 
aaacgattc aacgtctgt tgaaaatag atcagccgc 
cgaaaccgt cgctaaaca gctcctaac tcatttgga 
ttcatatca aaacaccga cgttctgtt atctccgtg 
gcaaaacca cgtttactc tagtccagt atcggaaaa 
tgaggaccc ctggagttc ggaacatga aactagact 
aagagagca cgtttcgag gcttaaagc cgaaagaag 
caatattca aggaactta cgtgacttt ggagtccac 
tcaagtgga aaatgtacc tgtcagacc atcaagtag 
tcaagaagc gagaaaaat ggatttgga aatggctgt 
tttgatgca ggagctaat gggactcag ggaagacaa 
agccgaact tatatcgaa gctatttat gttagaaaa 
agacaaaag tgccataga gttaaagtt gaagtacgt 
cgaagggca gcagaaagc ccaagcggc ggcgcttaa 
aaacctcca aggacagct gaaagacac ggaagcttt 
attggcctt agcgacaca gaataaaga gaggggtta 
ttcggatgc agagcatgc tcaaggtgt tgaactcga 
gctaatgca agcgctggc aagagaggc aagactgaa 
agttcgctt acaagaact tgtcacgac tttggaaca 
agtgacaaa aaatgccga agcgagact attggaagg 
gagagagat cgtgcacga tctgaatca cacaaatag 
tattttagc cgaaactgt agatagaaa caataagaa 
gtatcaatc caagtttaa gaaaatgga gcatcagat 
gttagcaat ggtagaaaa gcacaacct tcaagtcca 
gaatctcca acagagaat agccaccct tgaaactcc 
ccccaccaa tagttcaga caactctat caattctgt 
agctgtgta gtaaagtgt gttttaaat gaaattatt 
tttttattt ttcgttttg ttgatctct gttttaatc 
agtggcgaa ttgagaaat aaatcaatt tatttttgc 
cgaacctac tgactgcgc caattaata agcaaggcc 
aggttttat tacaacaat tacacccaa aatttaaaa 
aatacccca tcaattttt aatcttatc ttcgggcgt 
ttttggaga agtaatgtc gggttaatt cgccgctga 
ttttattat tgatgcatt taatgcggc ctgtatgta 
taccactaa attgtcgag gaagtatta tgagcagta 
taagatatt aaagttgta tatgcctag ctgtgatta 
tttatacat tgctgaatc ggaagtgcg actgattta 
tatggaaat gttactgca atggttgta ataatgtca 
ggcagtcaa gtgcttaag cttaattaa atattgttg 
tttattata aataatatc ggaatagtt tcccagatt 
tagtacaaa tttattata ttatactaa cgtcttgcc 
ataacgatg ttttttatg ataacatga ttgagagct 
gcaagtcaa tcatatcat tcattggag cgtgctgag 
aatgaagtc ataaattaa catctaaac cgatttcag 
gaaaattca tttactagt tctaatttt aacacattt 
taccacgtg ctaaaattc tattttact tacctttga 
aacgtaatg ggctaataa aacttattt ttgtaaatg 
aattttcca gtcattatt ttattaaaa cggaataac 
tataacata cattgattt gtattcagc tatgtattt 
attagttat tgaaataaa tatgtttac aatat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY