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Gene information
General information
TC001126 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 11349322..11360066
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001126
    iBeetle screen iB_02661

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva segment sometimes partiallynot present - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva dorsalnot closed - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages sometimes partiallynot present - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen segment sometimes potentiallyfused - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001126 RA
      MSLIEATPS ELFHEIIKL QLKKPKNWN WQLSTSKSS 
      PHIALPIIQ LYNSKGQLV VEAKDSGVT QRRSWHSLD 
      IRQKEKPTN AKKEYKEPT DRHLQRCRS DTLETDSRK 
      NRCANKLDS IRRRETQKS TNNKLTKSS SAALERRAN 
      ENRKSGSYN ESSIKGNTL HDLRKSSSD VLELKGNPR 
      FGETCARKP LFGENKEKR DFIPGHRRS RSDDLFRRK 
      LKTNEESLK RAELDGYKN FITPNVLLP NNASNIEVN 
      HFKQNRYKT RTSSAGTLI ISEESFNNR QRRRRRRDA 
      SIENSNNAQ NNAGPYGDT MPNTERTTN LPENRYASE 
      KIHKGSTRL SENGESGGL FYSRSDVFP LKLNKKCDD 
      NCNYLVTTP DSDNKNNKS FQAIMEKLE RKKRRKVRR 
      HYSDASTSP TALEKKYQH KRKGNGTKA MLVCISTAQ 
      ISDPLRNSS RIIYLTYRR NFSNSGIQL FRPKTTRRQ 
      SSGQFDQSP GFWWTAPLS GPIRPDMGS RRFRGSTCR 
      GKLVDPGA
    • >TC001126 RB
      MSLIEATPS ELFHEIIKL QLKKPKNWN WQLSTSKSS 
      PHIALPIIQ LYNSKGQLV VEAKDSGVT QRRSWHSLD 
      IRQKEKPTN AKKEYKEPT DRHLQRCRS DTLETDSRK 
      NRCANKLDS IRRRETQKS TNNKLTKSS SAALERRAN 
      ENRKSGSYN ESSIKGNTL HDLRKSSSD VLELKGNPR 
      FGETCARKP LFGENKEKR DFIPGHRRS RSDDLFRRK 
      LKTNEESLK RAELDGYKN FITPNVLLP NNASNIEVN 
      HFKQNRYKT RTSSAGTLI ISEESFNNR QRRRRRRDA 
      SIENSNNAQ NNAGPYGDT MPNTERTTN LPENRYASE 
      KIHKGSTRL SENGESGGL FYSRSDVFP LKLNKKCDD 
      NCNYLVTTP DSDNKNNKS FQAIMEKLE RKKRRKVRR 
      HYSDASTSP TALEKKYQH KRKGNGTKA GGSVTLKSW 
      GS
    CDS sequence
    • >TC001126 RA
      atgagcctg attgaagcg accccatcc gaactcttc 
      cacgagata atcaaattg cagttgaaa aagcccaag 
      aactggaac tggcaatta tcgacatcg aaatcttca 
      ccccacatt gcacttccg ataatacag ctttacaat 
      tcaaagggg caattggtg gtcgaagcc aaagacagt 
      ggcgtgacc cagcgccga agttggcac agtttggac 
      atccgacaa aaagaaaag cccacaaac gccaagaag 
      gaatataaa gaaccaaca gacagacat ttacagaga 
      tgtagaagc gacacatta gaaacggac tcgaggaaa 
      aaccgctgt gcaaacaaa ttggattcg ataagaagg 
      agagagacg cagaaatcg acgaataat aaattaact 
      aagagctcc agtgcggcg ctggagaga agggccaac 
      gaaaatagg aaatcagga agctacaat gagagttcg 
      attaagggg aacacactg catgatctc aggaaatcg 
      agcagcgac gttctggaa cttaaggga aatccccgt 
      ttcggcgaa acttgtgcc cggaagccc cttttcggc 
      gaaaacaaa gaaaaaagg gacttcatc ccaggccac 
      cgtcgaagt cgaagcgac gacctgttc cgccgaaaa 
      ttaaaaacc aacgaggaa agtttgaaa cgggccgaa 
      ctcgacggt tacaaaaac ttcatcacg ccgaatgtt 
      ttattgccc aataacgcc tctaatatc gaagtaaac 
      catttcaaa cagaacagg tacaaaaca aggacaagc 
      agcgccggc acgttaata attagcgag gaaagtttt 
      aacaaccgc cagcgacgc cgtcgccgt cgagacgcg 
      tcaatagaa aactcgaac aacgcccag aataatgcg 
      ggtccgtac ggcgacaca atgccaaat acggaaagg 
      accacaaat cttccggaa aatcgatac gcttccgaa 
      aaaatccac aaaggatcg acgagattg tccgaaaat 
      ggtgaatcg ggtggtttg ttctactcc cgcagtgat 
      gtgtttcct ttgaaactg aataaaaaa tgcgacgat 
      aattgtaat tacttagtg acaaccccc gacagtgat 
      aacaaaaac aataaaagt ttccaagca atcatggaa 
      aaattggaa aggaaaaaa cgcagaaaa gttcggaga 
      cattacagt gatgcatca acgtcgccc accgccttg 
      gaaaagaaa tatcaacat aaaagaaag ggaaacggg 
      acaaaagcg atgttagtg tgcatatcg accgcgcaa 
      atctcagat ccactccgc aattcgtcc cggattatt 
      tacctcact taccggaga aacttttcg aatagcggt 
      atccaactc tttcgccct aaaaccacc cgacgccag 
      tcctcgggt cagtttgat caatcaccc ggattttgg 
      tggacggcg cccttatct gggcccata aggccagat 
      atgggcagc cggcgcttc cgggggtcc acctgcaga 
      ggaaagctc gtcgatccg ggagcgtga
    • >TC001126 RB
      atgagcctg attgaagcg accccatcc gaactcttc 
      cacgagata atcaaattg cagttgaaa aagcccaag 
      aactggaac tggcaatta tcgacatcg aaatcttca 
      ccccacatt gcacttccg ataatacag ctttacaat 
      tcaaagggg caattggtg gtcgaagcc aaagacagt 
      ggcgtgacc cagcgccga agttggcac agtttggac 
      atccgacaa aaagaaaag cccacaaac gccaagaag 
      gaatataaa gaaccaaca gacagacat ttacagaga 
      tgtagaagc gacacatta gaaacggac tcgaggaaa 
      aaccgctgt gcaaacaaa ttggattcg ataagaagg 
      agagagacg cagaaatcg acgaataat aaattaact 
      aagagctcc agtgcggcg ctggagaga agggccaac 
      gaaaatagg aaatcagga agctacaat gagagttcg 
      attaagggg aacacactg catgatctc aggaaatcg 
      agcagcgac gttctggaa cttaaggga aatccccgt 
      ttcggcgaa acttgtgcc cggaagccc cttttcggc 
      gaaaacaaa gaaaaaagg gacttcatc ccaggccac 
      cgtcgaagt cgaagcgac gacctgttc cgccgaaaa 
      ttaaaaacc aacgaggaa agtttgaaa cgggccgaa 
      ctcgacggt tacaaaaac ttcatcacg ccgaatgtt 
      ttattgccc aataacgcc tctaatatc gaagtaaac 
      catttcaaa cagaacagg tacaaaaca aggacaagc 
      agcgccggc acgttaata attagcgag gaaagtttt 
      aacaaccgc cagcgacgc cgtcgccgt cgagacgcg 
      tcaatagaa aactcgaac aacgcccag aataatgcg 
      ggtccgtac ggcgacaca atgccaaat acggaaagg 
      accacaaat cttccggaa aatcgatac gcttccgaa 
      aaaatccac aaaggatcg acgagattg tccgaaaat 
      ggtgaatcg ggtggtttg ttctactcc cgcagtgat 
      gtgtttcct ttgaaactg aataaaaaa tgcgacgat 
      aattgtaat tacttagtg acaaccccc gacagtgat 
      aacaaaaac aataaaagt ttccaagca atcatggaa 
      aaattggaa aggaaaaaa cgcagaaaa gttcggaga 
      cattacagt gatgcatca acgtcgccc accgccttg 
      gaaaagaaa tatcaacat aaaagaaag ggaaacggg 
      acaaaagcc ggcggtagc gtaactctg aaaagttgg 
      ggctcctaa
    mRNA sequence
    • >TC001126 RA
      gataattta ttaagtgga ttgagtgaa atgtgttga 
      cgggaagtc cgctgaaaa tgagcctga ttgaagcga 
      ccccatccg aactcttcc acgagataa tcaaattgc 
      agttgaaaa agcccaaga actggaact ggcaattat 
      cgacatcga aatcttcac cccacattg cacttccga 
      taatacagc tttacaatt caaaggggc aattggtgg 
      tcgaagcca aagacagtg gcgtgaccc agcgccgaa 
      gttggcaca gtttggaca tccgacaaa aagaaaagc 
      ccacaaacg ccaagaagg aatataaag aaccaacag 
      acagacatt tacagagat gtagaagcg acacattag 
      aaacggact cgaggaaaa accgctgtg caaacaaat 
      tggattcga taagaagga gagagacgc agaaatcga 
      cgaataata aattaacta agagctcca gtgcggcgc 
      tggagagaa gggccaacg aaaatagga aatcaggaa 
      gctacaatg agagttcga ttaagggga acacactgc 
      atgatctca ggaaatcga gcagcgacg ttctggaac 
      ttaagggaa atccccgtt tcggcgaaa cttgtgccc 
      ggaagcccc ttttcggcg aaaacaaag aaaaaaggg 
      acttcatcc caggccacc gtcgaagtc gaagcgacg 
      acctgttcc gccgaaaat taaaaacca acgaggaaa 
      gtttgaaac gggccgaac tcgacggtt acaaaaact 
      tcatcacgc cgaatgttt tattgccca ataacgcct 
      ctaatatcg aagtaaacc atttcaaac agaacaggt 
      acaaaacaa ggacaagca gcgccggca cgttaataa 
      ttagcgagg aaagtttta acaaccgcc agcgacgcc 
      gtcgccgtc gagacgcgt caatagaaa actcgaaca 
      acgcccaga ataatgcgg gtccgtacg gcgacacaa 
      tgccaaata cggaaagga ccacaaatc ttccggaaa 
      atcgatacg cttccgaaa aaatccaca aaggatcga 
      cgagattgt ccgaaaatg gtgaatcgg gtggtttgt 
      tctactccc gcagtgatg tgtttcctt tgaaactga 
      ataaaaaat gcgacgata attgtaatt acttagtga 
      caacccccg acagtgata acaaaaaca ataaaagtt 
      tccaagcaa tcatggaaa aattggaaa ggaaaaaac 
      gcagaaaag ttcggagac attacagtg atgcatcaa 
      cgtcgccca ccgccttgg aaaagaaat atcaacata 
      aaagaaagg gaaacggga caaaagcga tgttagtgt 
      gcatatcga ccgcgcaaa tctcagatc cactccgca 
      attcgtccc ggattattt acctcactt accggagaa 
      acttttcga atagcggta tccaactct ttcgcccta 
      aaaccaccc gacgccagt cctcgggtc agtttgatc 
      aatcacccg gattttggt ggacggcgc ccttatctg 
      ggcccataa ggccagata tgggcagcc ggcgcttcc 
      gggggtcca cctgcagag gaaagctcg tcgatccgg 
      gagcgtgat cgtcgccta caaccctga cgagctcgc 
      aagcacccc agtggattg cgctggcgc acgaccggc 
      cggtcgacc tcgcctctt caccttgtt ccatcatca 
      agcgagttt gcgagagat agtgtgcta acgcggtcc 
      atacaccga actttcgtc gtcgaaacg acaaattgc 
      agtcgtatg tggccgtta acgataacg tgtgacggg 
      atttggtgc cgcatgccg gcctagaac ccacacagg 
      tacgtgctg ctttctgtt cagtgtatc acaacggct 
      gggttttgg tgattttca attattgcc gccgtgtga 
      acttggatc gctcaaatt aaaggtcgg tttttgccc 
      atgaacggc tggattgcc cccggtgta gggtccgac 
      tacgctccc agcaaacgt gacaggtat ttctctact 
      tctagatcc atcatcga
    • >TC001126 RB
      gataattta ttaagtgga ttgagtgaa atgtgttga 
      cgggaagtc cgctgaaaa tgagcctga ttgaagcga 
      ccccatccg aactcttcc acgagataa tcaaattgc 
      agttgaaaa agcccaaga actggaact ggcaattat 
      cgacatcga aatcttcac cccacattg cacttccga 
      taatacagc tttacaatt caaaggggc aattggtgg 
      tcgaagcca aagacagtg gcgtgaccc agcgccgaa 
      gttggcaca gtttggaca tccgacaaa aagaaaagc 
      ccacaaacg ccaagaagg aatataaag aaccaacag 
      acagacatt tacagagat gtagaagcg acacattag 
      aaacggact cgaggaaaa accgctgtg caaacaaat 
      tggattcga taagaagga gagagacgc agaaatcga 
      cgaataata aattaacta agagctcca gtgcggcgc 
      tggagagaa gggccaacg aaaatagga aatcaggaa 
      gctacaatg agagttcga ttaagggga acacactgc 
      atgatctca ggaaatcga gcagcgacg ttctggaac 
      ttaagggaa atccccgtt tcggcgaaa cttgtgccc 
      ggaagcccc ttttcggcg aaaacaaag aaaaaaggg 
      acttcatcc caggccacc gtcgaagtc gaagcgacg 
      acctgttcc gccgaaaat taaaaacca acgaggaaa 
      gtttgaaac gggccgaac tcgacggtt acaaaaact 
      tcatcacgc cgaatgttt tattgccca ataacgcct 
      ctaatatcg aagtaaacc atttcaaac agaacaggt 
      acaaaacaa ggacaagca gcgccggca cgttaataa 
      ttagcgagg aaagtttta acaaccgcc agcgacgcc 
      gtcgccgtc gagacgcgt caatagaaa actcgaaca 
      acgcccaga ataatgcgg gtccgtacg gcgacacaa 
      tgccaaata cggaaagga ccacaaatc ttccggaaa 
      atcgatacg cttccgaaa aaatccaca aaggatcga 
      cgagattgt ccgaaaatg gtgaatcgg gtggtttgt 
      tctactccc gcagtgatg tgtttcctt tgaaactga 
      ataaaaaat gcgacgata attgtaatt acttagtga 
      caacccccg acagtgata acaaaaaca ataaaagtt 
      tccaagcaa tcatggaaa aattggaaa ggaaaaaac 
      gcagaaaag ttcggagac attacagtg atgcatcaa 
      cgtcgccca ccgccttgg aaaagaaat atcaacata 
      aaagaaagg gaaacggga caaaagccg gcggtagcg 
      taactctga aaagttggg gctcctaag gcgcctcgc 
      tgtgattaa atagataaa tttggtcga atttgaatc 
      aggagagaa ggccacctc tgtgtctgt tactccctc 
      ttgagtcaa taaagatgc aaacggaca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY