Status
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Gene information
General information
TC034561 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 11294544..11302791
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034561
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02660

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034561 RA
      MKKKHQTPK VKIREKVQE ITDPEPPDS EEEEGFSKW 
      LRSREGIEN IKLFVLGNS ILICLLISW PEVKETLET 
      AYYMEEVRK YLHSQDPAQ QRNQVLKDK SLFLKMAPG 
      LETLKRGSI QLQRVAQKV VNDQIGSRH VLAVFIVTG 
      FVLCGVVEQ SASVALRAN STSSYSAAE FAEYVENTS 
      YITRYCQPP SNKSIVHVV RSIPHIEIP MTRTDETEA 
      VLRQAFMWG SLVSPLAAS RVAARVGAE RLFGAGVLG 
      TGVVAIMVP ASWLTPSHV AIRIVQGIF VGATFPAAH 
      MLAVTWIKT KHVSAFVSL YTAVNLGYA VVGILGTLL 
      VRSCGRDFL SYVIAMSSF LWYFLWWRF VEESLHPNR 
      PKRDEHRNL PWRRLLTSK PAWACGITV IGSQWADAT 
      LMLGVTKYL KLVYGFSIE YQDILQSLP HIGHFFAAI 
      TFGVVVDHV RQSGIVSTT TARKLFVYI SHFPPAALM 
      FVLGYTGCD PAAPAALYT AALAFTAAT PAGAFASAA 
      DIAPNFAGT VFGLCQTVG AAGLLAANY VVSEGLHGS 
      FAGWWRLVF GVSSAVLLT TAAFFMAMG SGSVQDWNA 
      PGDIEPELE PMEEPSRLD SVLE
    CDS sequence
    • >TC034561 RA
      atgaagaaa aaacaccaa acacccaaa gtcaaaatc 
      agagaaaaa gtacaggaa ataacggac cccgagccg 
      ccagattcc gaagaagag gaaggcttt tccaagtgg 
      ctacgttca agagaaggc atcgaaaat ataaaactc 
      ttcgtgtta ggaaattcg attttgatc tgtcttttg 
      atttcttgg ccggaagtg aaagaaacg ctcgaaaca 
      gcttattac atggaagaa gtgaggaag tatttgcat 
      tcgcaagac ccagctcag caaagaaat caagtgcta 
      aaagacaaa agtttattt ctaaaaatg gccccaggc 
      ttagaaact ttgaaaaga ggtagcatt caactccaa 
      agagttgca caaaaagtt gtcaacgat caaattgga 
      tcgagacac gttttggcg gtatttata gtcacaggc 
      ttcgtcctt tgcggagta gtcgaacaa agcgcatct 
      gtggcttta agggcaaac agtacttcc agttattca 
      gcagctgaa ttcgcagag tatgtcgaa aacaccagt 
      tatatcacg cgctattgt caaccaccg tctaacaaa 
      tcgatagtc cacgttgta cgatcaatt ccacacatc 
      gagattcca atgacgcga actgacgaa accgaagcc 
      gtgctgagg caagctttc atgtggggc agtcttgtc 
      tccccctta gcagcaagc cgagttgcc gcaagagtc 
      ggagcagaa cgtttattt ggggctggg gttcttgga 
      acaggggtt gtggcaatc atggtaccg gcatcgtgg 
      ctcacacca agccatgtt gccataagg atagttcaa 
      gggattttt gtgggtgca acttttccg gcagctcat 
      atgctggcg gtgacttgg atcaaaacc aaacatgtc 
      agtgcgttt gtttcttta tacacagct gttaatctc 
      ggatatgcg gtcgtcggg attttaggg acgctactg 
      gtcagaagt tgcggtcgc gatttctta agttatgtc 
      attgcaatg tcttcgttt ctgtggtac ttcctgtgg 
      tggagattc gttgaagag tctttacac cccaatcgg 
      ccaaaacga gacgaacat agaaacctg ccttggcgc 
      cgcttactc acttccaag ccagcttgg gcttgcggt 
      atcacagta atagggagc caatgggcc gatgctact 
      ctcatgtta ggtgttaca aaatattta aaactagtt 
      tacggattc tcaatcgaa tatcaagac atcctgcag 
      tcattacca cacattggc cattttttc gcagctatc 
      actttcgga gtggtcgta gaccacgta agacagtcg 
      ggaatcgtg tcgacaaca acagcacgg aaactattc 
      gtttacatt tcacatttt cctcctgcc gctctaatg 
      ttcgttttg ggttacacc ggctgcgat cctgcggct 
      cctgcagct ctctacact gctgctttg gcctttact 
      gcagccacc ccagctggg gcttttgcc agtgcagct 
      gacattgcc ccaaatttt gcaggcact gtgtttggt 
      ctgtgtcag accgtcggg gctgctggc cttctcgcc 
      gcaaattac gtggtgtcc gaaggttta cacggctca 
      tttgcaggt tggtggcgc ctggttttt ggcgtctca 
      tccgctgtg cttttgaca acagctgcg tttttcatg 
      gcgatgggg agcggctca gtgcaggac tggaacgcc 
      ccaggcgac atagagcca gagctggaa cctatggag 
      gaaccttct cggctggat tccgttctt gagtaa
    mRNA sequence
    • >TC034561 RA
      caaaaacat tgatttacg tcggctagc agattcctt 
      gcgaaggac aggcgatta aatatacac gatgggacc 
      atgaagaaa aaacaccaa acacccaaa gtcaaaatc 
      agagaaaaa gtacaggaa ataacggac cccgagccg 
      ccagattcc gaagaagag gaaggcttt tccaagtgg 
      ctacgttca agagaaggc atcgaaaat ataaaactc 
      ttcgtgtta ggaaattcg attttgatc tgtcttttg 
      atttcttgg ccggaagtg aaagaaacg ctcgaaaca 
      gcttattac atggaagaa gtgaggaag tatttgcat 
      tcgcaagac ccagctcag caaagaaat caagtgcta 
      aaagacaaa agtttattt ctaaaaatg gccccaggc 
      ttagaaact ttgaaaaga ggtagcatt caactccaa 
      agagttgca caaaaagtt gtcaacgat caaattgga 
      tcgagacac gttttggcg gtatttata gtcacaggc 
      ttcgtcctt tgcggagta gtcgaacaa agcgcatct 
      gtggcttta agggcaaac agtacttcc agttattca 
      gcagctgaa ttcgcagag tatgtcgaa aacaccagt 
      tatatcacg cgctattgt caaccaccg tctaacaaa 
      tcgatagtc cacgttgta cgatcaatt ccacacatc 
      gagattcca atgacgcga actgacgaa accgaagcc 
      gtgctgagg caagctttc atgtggggc agtcttgtc 
      tccccctta gcagcaagc cgagttgcc gcaagagtc 
      ggagcagaa cgtttattt ggggctggg gttcttgga 
      acaggggtt gtggcaatc atggtaccg gcatcgtgg 
      ctcacacca agccatgtt gccataagg atagttcaa 
      gggattttt gtgggtgca acttttccg gcagctcat 
      atgctggcg gtgacttgg atcaaaacc aaacatgtc 
      agtgcgttt gtttcttta tacacagct gttaatctc 
      ggatatgcg gtcgtcggg attttaggg acgctactg 
      gtcagaagt tgcggtcgc gatttctta agttatgtc 
      attgcaatg tcttcgttt ctgtggtac ttcctgtgg 
      tggagattc gttgaagag tctttacac cccaatcgg 
      ccaaaacga gacgaacat agaaacctg ccttggcgc 
      cgcttactc acttccaag ccagcttgg gcttgcggt 
      atcacagta atagggagc caatgggcc gatgctact 
      ctcatgtta ggtgttaca aaatattta aaactagtt 
      tacggattc tcaatcgaa tatcaagac atcctgcag 
      tcattacca cacattggc cattttttc gcagctatc 
      actttcgga gtggtcgta gaccacgta agacagtcg 
      ggaatcgtg tcgacaaca acagcacgg aaactattc 
      gtttacatt tcacatttt cctcctgcc gctctaatg 
      ttcgttttg ggttacacc ggctgcgat cctgcggct 
      cctgcagct ctctacact gctgctttg gcctttact 
      gcagccacc ccagctggg gcttttgcc agtgcagct 
      gacattgcc ccaaatttt gcaggcact gtgtttggt 
      ctgtgtcag accgtcggg gctgctggc cttctcgcc 
      gcaaattac gtggtgtcc gaaggttta cacggctca 
      tttgcaggt tggtggcgc ctggttttt ggcgtctca 
      tccgctgtg cttttgaca acagctgcg tttttcatg 
      gcgatgggg agcggctca gtgcaggac tggaacgcc 
      ccaggcgac atagagcca gagctggaa cctatggag 
      gaaccttct cggctggat tccgttctt gagtaagaa 
      caggggaaa atcggatcg tgcgacacc agacaagtg 
      ctttcatac acc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY