Status
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Gene information
General information
TC000451 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 11103923..11130210
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000451
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02659

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 12.5% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    Pupal morphology
    abdomen seta dorsal not present - Number of animals showing the phenotype: 9

    abdomen gin-trap setae not present - Number of animals showing the phenotype: 9

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000451 RA
      MSRGYYNTG APFLYSGGE AVGPGMDCL QLRQPPGPF 
      HYLINEQAK SLVALQELQ HEVGALLQF RDLVIETFP 
      NLRTKLASS TPATMTSSH QNSNIPVSI RTADWTPGV 
      RVRKKLASK DETRKKGGD IAVQDSGFS TETSSKETH 
      SASSTAPTA AGSRSSCGS FKFDETEDE LWNLLDVIH 
      RKGTRLKDE VEALQGAIR EQGNMREEN DFQRVLFTG 
      SADDIRQLR HERDLLLDR LAEMEAEVL AGRVHTSRL 
      QEDLENLLS AKHDLEEQL KAVVSQRGE VNSRIHDLH 
      LQFVTKSGA SSPDGDKSK VLIGDSLSR RDSTASQRT 
      QRRNSRSAL DLVLGDRIP KVKVPDSKK IAAILKESD 
      PLVLQKHLL STTVQNQVL YQQLDTAAR VEIGLADKL 
      DKAREENDE LRFQLEDKN IELEGTRAR VRMLEQLQK 
      SITNTSPDV IPDHPISRS EITTASMKA MSPLPLNLQ 
      MDHSSSTES AHDQAETNR KNSDNSKRR PSKIPLKSY 
      TAPKPPGGK HSPAPGARS RSGESPGRP HSAQSWRNR 
      SEGNSLSGS KSNSSLPKS RNGSLVSAK DSLSSKLRS 
      SDSLSKLSS SNNSFGSNR GTMRKTSTP VKWTNNSKD 
      VTDKVRPKT TSFWNFFKL HNGQT
    • >TC000451 RB
      MRICYSVGG EAVGPGMDC LQLRQPPGP FHYLINEQA 
      KSLVALQEL QHEVGALLQ FRDLVIETF PNLRTKLAS 
      STPATMTSS HQNSNIPVS IRTADWTPG VRVRKKLAS 
      KDETRKKGG DIAVQDSGF STETSSKET HSASSTAPT 
      AAGSRSSCG SFKFDETED ELWNLLDVI HRKGTRLKD 
      EVEALQGAI REQGNMREE NDFQRVLFT GSADDIRQL 
      RHERDLLLD RLAEMEAEV LAGRVHTSR LQEDLENLL 
      SAKHDLEEQ LKAVVSQRG EVNSRIHDL HLQFVTKSG 
      ASSPDGDKS KVLIGDSLS RRDSTASQR TQRRNSRSA 
      LDLVLGDRI PKVKVPDSK KIAAILKES DPLVLQKHL 
      LSTTVQNQV LYQQLDTAA RVEIGLADK LDKAREEND 
      ELRFQLEDK NIELEGTRA RVRMLEQLQ KSITNTSPD 
      VIPDHPISR SEITTASMK AMSPLPLNL QMDHSSSTE 
      SAHDQAETN RKNSDNSKR RPSKIPLKS YTAPKPPGG 
      KHSPAPGAR SRSGESPGR PHSAQSWRN RSEGNSLSG 
      SKSNSSLPK SRNGSLVSA KDSLSSKLR SSDSLSKLS 
      SSNNSFGSN RGTMRKTST PVKWTNNSK DVTDKVRPK 
      TTSFWNFFK LHNGQT
    CDS sequence
    • >TC000451 RA
      atgagtagg ggatactac aacaccggg gcccccttt 
      ctgtattcg ggtggcgag gcggtgggc cctggcatg 
      gactgcctc cagctgcgc cagcccccc ggcccattc 
      cactacctg atcaacgag caggcgaag tccctcgtg 
      gccctccaa gagctccag catgaagtg ggcgctctt 
      ctgcagttt cgcgatctc gtcatcgag acatttcca 
      aatctgcgg accaaactc gcctcgtcg acgcctgcc 
      accatgact tcgtcgcat caaaactcc aacatcccg 
      gtctcgatt cggacagcg gactggaca cccggcgtc 
      cgcgtccgc aaaaaactc gctagcaag gacgagacg 
      cgcaaaaag gggggcgat atcgcggtg caagactcc 
      gggttcagc acggagacg tcgagtaag gagacgcac 
      agcgcctcg tccacggca ccgacggct gcagggagt 
      cgcagttct tgcggatcc ttcaagttt gatgagact 
      gaagacgag ctgtggaac cttctggat gtgatccac 
      cggaagggg acgcggttg aaggacgag gtggaggcc 
      ctgcagggg gcgatcagg gagcagggg aacatgagg 
      gaggagaat gattttcag agggttttg tttacgggg 
      tctgctgat gatatcagg cagttgcgg catgagcgg 
      gacctcctg ttggatcgt ttggcggag atggaggct 
      gaggtcttg gccggacgg gtgcacacg tcgaggttg 
      caggaagat ttggagaat cttttgtcg gccaaacac 
      gacttggag gaacagttg aaggcggtg gtgtcgcag 
      aggggtgaa gtgaattca cgcatacat gacttacat 
      ttgcaattt gtgactaaa agtggggct agttcgccg 
      gatggtgat aagtccaag gtgctaata ggtgatagt 
      ttgagtaga agagattca acggctagt caaaggaca 
      caaaggaga aatagtcga agtgcattg gatctagtg 
      cttggtgat agaataccc aaagttaaa gtgcccgat 
      tcgaagaaa attgctgcc attttgaag gaaagcgat 
      ccgctggtt ctgcagaaa catctcctc tcgaccact 
      gtccaaaat caggtttta tatcaacag cttgacacc 
      gcagcgcga gtagaaatc ggtcttgct gataaatta 
      gacaaggca cgcgaagaa aacgatgag cttcgattt 
      cagctggag gacaagaac atagaatta gagggaacg 
      cgtgcccgt gttcgaatg ctggaacaa ctccaaaag 
      tcgataacc aacacctcc cccgacgtc atccccgac 
      catcccatc tcccgtagc gaaatcacc acggctagc 
      atgaaagcg atgagtcct ctacctctc aacctccaa 
      atggaccat tcgagcagc accgagtcg gcccacgac 
      caggccgag accaaccgc aagaacagc gacaacagc 
      aaacggcgc cccagcaaa atcccgctg aagagctac 
      acggcgccg aagccacca ggaggtaaa cacagtccc 
      gcacccggt gcacgtagt cgtagcggc gaatcgccg 
      ggtagaccg cactctgca cagtcctgg aggaacagg 
      agcgagggc aacagcttg tcgggcagc aagagcaac 
      tcgtccctg ccgaaatcc aggaacggg tcgctcgtt 
      tcggccaag gactcgctt tccagcaag cttaggagt 
      agcgactcg ctttccaag ctctcgtcg agtaacaat 
      agctttggg agtaacagg ggcaccatg aggaagacc 
      tccacgccg gttaagtgg accaataac tccaaagac 
      gttaccgat aaggtacga ccaaaaacc acttcattc 
      tggaatttc ttcaaacta cacaacggt cagacgtag
    • >TC000451 RB
      atgaggatc tgctacagc gtgggtggc gaggcggtg 
      ggccctggc atggactgc ctccagctg cgccagccc 
      cccggccca ttccactac ctgatcaac gagcaggcg 
      aagtccctc gtggccctc caagagctc cagcatgaa 
      gtgggcgct cttctgcag tttcgcgat ctcgtcatc 
      gagacattt ccaaatctg cggaccaaa ctcgcctcg 
      tcgacgcct gccaccatg acttcgtcg catcaaaac 
      tccaacatc ccggtctcg attcggaca gcggactgg 
      acacccggc gtccgcgtc cgcaaaaaa ctcgctagc 
      aaggacgag acgcgcaaa aaggggggc gatatcgcg 
      gtgcaagac tccgggttc agcacggag acgtcgagt 
      aaggagacg cacagcgcc tcgtccacg gcaccgacg 
      gctgcaggg agtcgcagt tcttgcgga tccttcaag 
      tttgatgag actgaagac gagctgtgg aaccttctg 
      gatgtgatc caccggaag gggacgcgg ttgaaggac 
      gaggtggag gccctgcag ggggcgatc agggagcag 
      gggaacatg agggaggag aatgatttt cagagggtt 
      ttgtttacg gggtctgct gatgatatc aggcagttg 
      cggcatgag cgggacctc ctgttggat cgtttggcg 
      gagatggag gctgaggtc ttggccgga cgggtgcac 
      acgtcgagg ttgcaggaa gatttggag aatcttttg 
      tcggccaaa cacgacttg gaggaacag ttgaaggcg 
      gtggtgtcg cagaggggt gaagtgaat tcacgcata 
      catgactta catttgcaa tttgtgact aaaagtggg 
      gctagttcg ccggatggt gataagtcc aaggtgcta 
      ataggtgat agtttgagt agaagagat tcaacggct 
      agtcaaagg acacaaagg agaaatagt cgaagtgca 
      ttggatcta gtgcttggt gatagaata cccaaagtt 
      aaagtgccc gattcgaag aaaattgct gccattttg 
      aaggaaagc gatccgctg gttctgcag aaacatctc 
      ctctcgacc actgtccaa aatcaggtt ttatatcaa 
      cagcttgac accgcagcg cgagtagaa atcggtctt 
      gctgataaa ttagacaag gcacgcgaa gaaaacgat 
      gagcttcga tttcagctg gaggacaag aacatagaa 
      ttagaggga acgcgtgcc cgtgttcga atgctggaa 
      caactccaa aagtcgata accaacacc tcccccgac 
      gtcatcccc gaccatccc atctcccgt agcgaaatc 
      accacggct agcatgaaa gcgatgagt cctctacct 
      ctcaacctc caaatggac cattcgagc agcaccgag 
      tcggcccac gaccaggcc gagaccaac cgcaagaac 
      agcgacaac agcaaacgg cgccccagc aaaatcccg 
      ctgaagagc tacacggcg ccgaagcca ccaggaggt 
      aaacacagt cccgcaccc ggtgcacgt agtcgtagc 
      ggcgaatcg ccgggtaga ccgcactct gcacagtcc 
      tggaggaac aggagcgag ggcaacagc ttgtcgggc 
      agcaagagc aactcgtcc ctgccgaaa tccaggaac 
      gggtcgctc gtttcggcc aaggactcg ctttccagc 
      aagcttagg agtagcgac tcgctttcc aagctctcg 
      tcgagtaac aatagcttt gggagtaac aggggcacc 
      atgaggaag acctccacg ccggttaag tggaccaat 
      aactccaaa gacgttacc gataaggta cgaccaaaa 
      accacttca ttctggaat ttcttcaaa ctacacaac 
      ggtcagacg tag
    mRNA sequence
    • >TC000451 RA
      cgtcaattt gacaggtaa ccaaacaat cgggaaatc 
      gccagttta tgagtgagt cacagtgga taaaagtgc 
      agttagtgg tgttttgta catatttac aaattatct 
      atcgtccac aaaatgcgc taatcaggc attaaattg 
      cctcacgtc acggcattt tacataagg caacggaac 
      cagccaaca atcttgcag tgaaaaact acctgacct 
      tcttaactt atcgttgct taattaacg tataaataa 
      ctattgttt gaaaatggt gcgtttttc ggtggaaaa 
      tatgtgaac tggcgaaat gggtcactt ccggacgag 
      ggtgcaccc ctactgaca agatgagta ggggatact 
      acaacaccg gggccccct ttctgtatt cgggtggcg 
      aggcggtgg gccctggca tggactgcc tccagctgc 
      gccagcccc ccggcccat tccactacc tgatcaacg 
      agcaggcga agtccctcg tggccctcc aagagctcc 
      agcatgaag tgggcgctc ttctgcagt ttcgcgatc 
      tcgtcatcg agacatttc caaatctgc ggaccaaac 
      tcgcctcgt cgacgcctg ccaccatga cttcgtcgc 
      atcaaaact ccaacatcc cggtctcga ttcggacag 
      cggactgga cacccggcg tccgcgtcc gcaaaaaac 
      tcgctagca aggacgaga cgcgcaaaa aggggggcg 
      atatcgcgg tgcaagact ccgggttca gcacggaga 
      cgtcgagta aggagacgc acagcgcct cgtccacgg 
      caccgacgg ctgcaggga gtcgcagtt cttgcggat 
      ccttcaagt ttgatgaga ctgaagacg agctgtgga 
      accttctgg atgtgatcc accggaagg ggacgcggt 
      tgaaggacg aggtggagg ccctgcagg gggcgatca 
      gggagcagg ggaacatga gggaggaga atgattttc 
      agagggttt tgtttacgg ggtctgctg atgatatca 
      ggcagttgc ggcatgagc gggacctcc tgttggatc 
      gtttggcgg agatggagg ctgaggtct tggccggac 
      gggtgcaca cgtcgaggt tgcaggaag atttggaga 
      atcttttgt cggccaaac acgacttgg aggaacagt 
      tgaaggcgg tggtgtcgc agaggggtg aagtgaatt 
      cacgcatac atgacttac atttgcaat ttgtgacta 
      aaagtgggg ctagttcgc cggatggtg ataagtcca 
      aggtgctaa taggtgata gtttgagta gaagagatt 
      caacggcta gtcaaagga cacaaagga gaaatagtc 
      gaagtgcat tggatctag tgcttggtg atagaatac 
      ccaaagtta aagtgcccg attcgaaga aaattgctg 
      ccattttga aggaaagcg atccgctgg ttctgcaga 
      aacatctcc tctcgacca ctgtccaaa atcaggttt 
      tatatcaac agcttgaca ccgcagcgc gagtagaaa 
      tcggtcttg ctgataaat tagacaagg cacgcgaag 
      aaaacgatg agcttcgat ttcagctgg aggacaaga 
      acatagaat tagagggaa cgcgtgccc gtgttcgaa 
      tgctggaac aactccaaa agtcgataa ccaacacct 
      cccccgacg tcatccccg accatccca tctcccgta 
      gcgaaatca ccacggcta gcatgaaag cgatgagtc 
      ctctacctc tcaacctcc aaatggacc attcgagca 
      gcaccgagt cggcccacg accaggccg agaccaacc 
      gcaagaaca gcgacaaca gcaaacggc gccccagca 
      aaatcccgc tgaagagct acacggcgc cgaagccac 
      caggaggta aacacagtc ccgcacccg gtgcacgta 
      gtcgtagcg gcgaatcgc cgggtagac cgcactctg 
      cacagtcct ggaggaaca ggagcgagg gcaacagct 
      tgtcgggca gcaagagca actcgtccc tgccgaaat 
      ccaggaacg ggtcgctcg tttcggcca aggactcgc 
      tttccagca agcttagga gtagcgact cgctttcca 
      agctctcgt cgagtaaca atagctttg ggagtaaca 
      ggggcacca tgaggaaga cctccacgc cggttaagt 
      ggaccaata actccaaag acgttaccg ataaggtac 
      gaccaaaaa ccacttcat tctggaatt tcttcaaac 
      tacacaacg gtcagacgt aggagtgcc gccattatt 
      atttagttg ctctcggcc gtctcgaga cgtgcagta 
      agtgacgct tgttttttt ttatttgct ttgtccgct 
      tatcaaatc ttgtagatc cattgctca cgtaagtag 
      agagtagta ccttgttcc cctctccct ttttttctg 
      aagcgtttt attcggtaa atcaatcgc atgctaatg 
      aaatttaaa actgattca gaagcgggg tttcgtgga 
      ctccctgct gaaatcttc gatgtgctg caaaaaacc 
      ggtactcgt attcatttg tctgaagtc attgtgacc 
      gagaaaaga actgtagcg aagacattt tgaaatatt 
      agataccgt atgagaaca aaatc
    • >TC000451 RB
      tccatcatg cctccgttt cagagtgat catgatcag 
      tgccttgct gttcggggg gcatcaacc tcattcttt 
      acaaaatca tctgtttat agtgctatg tctgccatg 
      actgatgtt tcttacatg tgtatttta taattttct 
      attgtcgga gtgattcga tcagatgag gatctgcta 
      cagcgtggg tggcgaggc ggtgggccc tggcatgga 
      ctgcctcca gctgcgcca gccccccgg cccattcca 
      ctacctgat caacgagca ggcgaagtc cctcgtggc 
      cctccaaga gctccagca tgaagtggg cgctcttct 
      gcagtttcg cgatctcgt catcgagac atttccaaa 
      tctgcggac caaactcgc ctcgtcgac gcctgccac 
      catgacttc gtcgcatca aaactccaa catcccggt 
      ctcgattcg gacagcgga ctggacacc cggcgtccg 
      cgtccgcaa aaaactcgc tagcaagga cgagacgcg 
      caaaaaggg gggcgatat cgcggtgca agactccgg 
      gttcagcac ggagacgtc gagtaagga gacgcacag 
      cgcctcgtc cacggcacc gacggctgc agggagtcg 
      cagttcttg cggatcctt caagtttga tgagactga 
      agacgagct gtggaacct tctggatgt gatccaccg 
      gaaggggac gcggttgaa ggacgaggt ggaggccct 
      gcagggggc gatcaggga gcaggggaa catgaggga 
      ggagaatga ttttcagag ggttttgtt tacggggtc 
      tgctgatga tatcaggca gttgcggca tgagcggga 
      cctcctgtt ggatcgttt ggcggagat ggaggctga 
      ggtcttggc cggacgggt gcacacgtc gaggttgca 
      ggaagattt ggagaatct tttgtcggc caaacacga 
      cttggagga acagttgaa ggcggtggt gtcgcagag 
      gggtgaagt gaattcacg catacatga cttacattt 
      gcaatttgt gactaaaag tggggctag ttcgccgga 
      tggtgataa gtccaaggt gctaatagg tgatagttt 
      gagtagaag agattcaac ggctagtca aaggacaca 
      aaggagaaa tagtcgaag tgcattgga tctagtgct 
      tggtgatag aatacccaa agttaaagt gcccgattc 
      gaagaaaat tgctgccat tttgaagga aagcgatcc 
      gctggttct gcagaaaca tctcctctc gaccactgt 
      ccaaaatca ggttttata tcaacagct tgacaccgc 
      agcgcgagt agaaatcgg tcttgctga taaattaga 
      caaggcacg cgaagaaaa cgatgagct tcgatttca 
      gctggagga caagaacat agaattaga gggaacgcg 
      tgcccgtgt tcgaatgct ggaacaact ccaaaagtc 
      gataaccaa cacctcccc cgacgtcat ccccgacca 
      tcccatctc ccgtagcga aatcaccac ggctagcat 
      gaaagcgat gagtcctct acctctcaa cctccaaat 
      ggaccattc gagcagcac cgagtcggc ccacgacca 
      ggccgagac caaccgcaa gaacagcga caacagcaa 
      acggcgccc cagcaaaat cccgctgaa gagctacac 
      ggcgccgaa gccaccagg aggtaaaca cagtcccgc 
      acccggtgc acgtagtcg tagcggcga atcgccggg 
      tagaccgca ctctgcaca gtcctggag gaacaggag 
      cgagggcaa cagcttgtc gggcagcaa gagcaactc 
      gtccctgcc gaaatccag gaacgggtc gctcgtttc 
      ggccaagga ctcgctttc cagcaagct taggagtag 
      cgactcgct ttccaagct ctcgtcgag taacaatag 
      ctttgggag taacagggg caccatgag gaagacctc 
      cacgccggt taagtggac caataactc caaagacgt 
      taccgataa ggtacgacc aaaaaccac ttcattctg 
      gaatttctt caaactaca caacggtca gacgtagga 
      gtgccgcca ttattattt agttgctct cggccgtct 
      cgagacgtg cagtaagtg acgcttgtt tttttttat 
      ttgctttgt ccgcttatc aaatcttgt agatccatt 
      gctcacgta agtagagag tagtacctt gttcccctc 
      tcccttttt ttctgaagc gttttattc ggtaaatca 
      atcgcatgc taatgaaat ttaaaactg attcagaag 
      cggggtttc gtggactcc ctgctgaaa tcttcgatg 
      tgctgcaaa aaaccggta ctcgtattc atttgtctg 
      aagtcattg tgaccgaga aaagaactg tagcgaaga 
      cattttgaa atattagat accgtatga gaacaaaat 
      c
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