| General information | |||
| TC000451 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 11103923..11130210 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC000451 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02659 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 12.5% (includes death as larva, prepupa, pupa, adult)
abdomen gin-trap setae not present - Number of animals showing the phenotype: 9
- >TC000451 RA
MSRGYYNTG APFLYSGGE AVGPGMDCL QLRQPPGPF HYLINEQAK SLVALQELQ HEVGALLQF RDLVIETFP NLRTKLASS TPATMTSSH QNSNIPVSI RTADWTPGV RVRKKLASK DETRKKGGD IAVQDSGFS TETSSKETH SASSTAPTA AGSRSSCGS FKFDETEDE LWNLLDVIH RKGTRLKDE VEALQGAIR EQGNMREEN DFQRVLFTG SADDIRQLR HERDLLLDR LAEMEAEVL AGRVHTSRL QEDLENLLS AKHDLEEQL KAVVSQRGE VNSRIHDLH LQFVTKSGA SSPDGDKSK VLIGDSLSR RDSTASQRT QRRNSRSAL DLVLGDRIP KVKVPDSKK IAAILKESD PLVLQKHLL STTVQNQVL YQQLDTAAR VEIGLADKL DKAREENDE LRFQLEDKN IELEGTRAR VRMLEQLQK SITNTSPDV IPDHPISRS EITTASMKA MSPLPLNLQ MDHSSSTES AHDQAETNR KNSDNSKRR PSKIPLKSY TAPKPPGGK HSPAPGARS RSGESPGRP HSAQSWRNR SEGNSLSGS KSNSSLPKS RNGSLVSAK DSLSSKLRS SDSLSKLSS SNNSFGSNR GTMRKTSTP VKWTNNSKD VTDKVRPKT TSFWNFFKL HNGQT
- >TC000451 RB
MRICYSVGG EAVGPGMDC LQLRQPPGP FHYLINEQA KSLVALQEL QHEVGALLQ FRDLVIETF PNLRTKLAS STPATMTSS HQNSNIPVS IRTADWTPG VRVRKKLAS KDETRKKGG DIAVQDSGF STETSSKET HSASSTAPT AAGSRSSCG SFKFDETED ELWNLLDVI HRKGTRLKD EVEALQGAI REQGNMREE NDFQRVLFT GSADDIRQL RHERDLLLD RLAEMEAEV LAGRVHTSR LQEDLENLL SAKHDLEEQ LKAVVSQRG EVNSRIHDL HLQFVTKSG ASSPDGDKS KVLIGDSLS RRDSTASQR TQRRNSRSA LDLVLGDRI PKVKVPDSK KIAAILKES DPLVLQKHL LSTTVQNQV LYQQLDTAA RVEIGLADK LDKAREEND ELRFQLEDK NIELEGTRA RVRMLEQLQ KSITNTSPD VIPDHPISR SEITTASMK AMSPLPLNL QMDHSSSTE SAHDQAETN RKNSDNSKR RPSKIPLKS YTAPKPPGG KHSPAPGAR SRSGESPGR PHSAQSWRN RSEGNSLSG SKSNSSLPK SRNGSLVSA KDSLSSKLR SSDSLSKLS SSNNSFGSN RGTMRKTST PVKWTNNSK DVTDKVRPK TTSFWNFFK LHNGQT
- >TC000451 RA
atgagtagg ggatactac aacaccggg gcccccttt ctgtattcg ggtggcgag gcggtgggc cctggcatg gactgcctc cagctgcgc cagcccccc ggcccattc cactacctg atcaacgag caggcgaag tccctcgtg gccctccaa gagctccag catgaagtg ggcgctctt ctgcagttt cgcgatctc gtcatcgag acatttcca aatctgcgg accaaactc gcctcgtcg acgcctgcc accatgact tcgtcgcat caaaactcc aacatcccg gtctcgatt cggacagcg gactggaca cccggcgtc cgcgtccgc aaaaaactc gctagcaag gacgagacg cgcaaaaag gggggcgat atcgcggtg caagactcc gggttcagc acggagacg tcgagtaag gagacgcac agcgcctcg tccacggca ccgacggct gcagggagt cgcagttct tgcggatcc ttcaagttt gatgagact gaagacgag ctgtggaac cttctggat gtgatccac cggaagggg acgcggttg aaggacgag gtggaggcc ctgcagggg gcgatcagg gagcagggg aacatgagg gaggagaat gattttcag agggttttg tttacgggg tctgctgat gatatcagg cagttgcgg catgagcgg gacctcctg ttggatcgt ttggcggag atggaggct gaggtcttg gccggacgg gtgcacacg tcgaggttg caggaagat ttggagaat cttttgtcg gccaaacac gacttggag gaacagttg aaggcggtg gtgtcgcag aggggtgaa gtgaattca cgcatacat gacttacat ttgcaattt gtgactaaa agtggggct agttcgccg gatggtgat aagtccaag gtgctaata ggtgatagt ttgagtaga agagattca acggctagt caaaggaca caaaggaga aatagtcga agtgcattg gatctagtg cttggtgat agaataccc aaagttaaa gtgcccgat tcgaagaaa attgctgcc attttgaag gaaagcgat ccgctggtt ctgcagaaa catctcctc tcgaccact gtccaaaat caggtttta tatcaacag cttgacacc gcagcgcga gtagaaatc ggtcttgct gataaatta gacaaggca cgcgaagaa aacgatgag cttcgattt cagctggag gacaagaac atagaatta gagggaacg cgtgcccgt gttcgaatg ctggaacaa ctccaaaag tcgataacc aacacctcc cccgacgtc atccccgac catcccatc tcccgtagc gaaatcacc acggctagc atgaaagcg atgagtcct ctacctctc aacctccaa atggaccat tcgagcagc accgagtcg gcccacgac caggccgag accaaccgc aagaacagc gacaacagc aaacggcgc cccagcaaa atcccgctg aagagctac acggcgccg aagccacca ggaggtaaa cacagtccc gcacccggt gcacgtagt cgtagcggc gaatcgccg ggtagaccg cactctgca cagtcctgg aggaacagg agcgagggc aacagcttg tcgggcagc aagagcaac tcgtccctg ccgaaatcc aggaacggg tcgctcgtt tcggccaag gactcgctt tccagcaag cttaggagt agcgactcg ctttccaag ctctcgtcg agtaacaat agctttggg agtaacagg ggcaccatg aggaagacc tccacgccg gttaagtgg accaataac tccaaagac gttaccgat aaggtacga ccaaaaacc acttcattc tggaatttc ttcaaacta cacaacggt cagacgtag
- >TC000451 RB
atgaggatc tgctacagc gtgggtggc gaggcggtg ggccctggc atggactgc ctccagctg cgccagccc cccggccca ttccactac ctgatcaac gagcaggcg aagtccctc gtggccctc caagagctc cagcatgaa gtgggcgct cttctgcag tttcgcgat ctcgtcatc gagacattt ccaaatctg cggaccaaa ctcgcctcg tcgacgcct gccaccatg acttcgtcg catcaaaac tccaacatc ccggtctcg attcggaca gcggactgg acacccggc gtccgcgtc cgcaaaaaa ctcgctagc aaggacgag acgcgcaaa aaggggggc gatatcgcg gtgcaagac tccgggttc agcacggag acgtcgagt aaggagacg cacagcgcc tcgtccacg gcaccgacg gctgcaggg agtcgcagt tcttgcgga tccttcaag tttgatgag actgaagac gagctgtgg aaccttctg gatgtgatc caccggaag gggacgcgg ttgaaggac gaggtggag gccctgcag ggggcgatc agggagcag gggaacatg agggaggag aatgatttt cagagggtt ttgtttacg gggtctgct gatgatatc aggcagttg cggcatgag cgggacctc ctgttggat cgtttggcg gagatggag gctgaggtc ttggccgga cgggtgcac acgtcgagg ttgcaggaa gatttggag aatcttttg tcggccaaa cacgacttg gaggaacag ttgaaggcg gtggtgtcg cagaggggt gaagtgaat tcacgcata catgactta catttgcaa tttgtgact aaaagtggg gctagttcg ccggatggt gataagtcc aaggtgcta ataggtgat agtttgagt agaagagat tcaacggct agtcaaagg acacaaagg agaaatagt cgaagtgca ttggatcta gtgcttggt gatagaata cccaaagtt aaagtgccc gattcgaag aaaattgct gccattttg aaggaaagc gatccgctg gttctgcag aaacatctc ctctcgacc actgtccaa aatcaggtt ttatatcaa cagcttgac accgcagcg cgagtagaa atcggtctt gctgataaa ttagacaag gcacgcgaa gaaaacgat gagcttcga tttcagctg gaggacaag aacatagaa ttagaggga acgcgtgcc cgtgttcga atgctggaa caactccaa aagtcgata accaacacc tcccccgac gtcatcccc gaccatccc atctcccgt agcgaaatc accacggct agcatgaaa gcgatgagt cctctacct ctcaacctc caaatggac cattcgagc agcaccgag tcggcccac gaccaggcc gagaccaac cgcaagaac agcgacaac agcaaacgg cgccccagc aaaatcccg ctgaagagc tacacggcg ccgaagcca ccaggaggt aaacacagt cccgcaccc ggtgcacgt agtcgtagc ggcgaatcg ccgggtaga ccgcactct gcacagtcc tggaggaac aggagcgag ggcaacagc ttgtcgggc agcaagagc aactcgtcc ctgccgaaa tccaggaac gggtcgctc gtttcggcc aaggactcg ctttccagc aagcttagg agtagcgac tcgctttcc aagctctcg tcgagtaac aatagcttt gggagtaac aggggcacc atgaggaag acctccacg ccggttaag tggaccaat aactccaaa gacgttacc gataaggta cgaccaaaa accacttca ttctggaat ttcttcaaa ctacacaac ggtcagacg tag
- >TC000451 RA
cgtcaattt gacaggtaa ccaaacaat cgggaaatc gccagttta tgagtgagt cacagtgga taaaagtgc agttagtgg tgttttgta catatttac aaattatct atcgtccac aaaatgcgc taatcaggc attaaattg cctcacgtc acggcattt tacataagg caacggaac cagccaaca atcttgcag tgaaaaact acctgacct tcttaactt atcgttgct taattaacg tataaataa ctattgttt gaaaatggt gcgtttttc ggtggaaaa tatgtgaac tggcgaaat gggtcactt ccggacgag ggtgcaccc ctactgaca agatgagta ggggatact acaacaccg gggccccct ttctgtatt cgggtggcg aggcggtgg gccctggca tggactgcc tccagctgc gccagcccc ccggcccat tccactacc tgatcaacg agcaggcga agtccctcg tggccctcc aagagctcc agcatgaag tgggcgctc ttctgcagt ttcgcgatc tcgtcatcg agacatttc caaatctgc ggaccaaac tcgcctcgt cgacgcctg ccaccatga cttcgtcgc atcaaaact ccaacatcc cggtctcga ttcggacag cggactgga cacccggcg tccgcgtcc gcaaaaaac tcgctagca aggacgaga cgcgcaaaa aggggggcg atatcgcgg tgcaagact ccgggttca gcacggaga cgtcgagta aggagacgc acagcgcct cgtccacgg caccgacgg ctgcaggga gtcgcagtt cttgcggat ccttcaagt ttgatgaga ctgaagacg agctgtgga accttctgg atgtgatcc accggaagg ggacgcggt tgaaggacg aggtggagg ccctgcagg gggcgatca gggagcagg ggaacatga gggaggaga atgattttc agagggttt tgtttacgg ggtctgctg atgatatca ggcagttgc ggcatgagc gggacctcc tgttggatc gtttggcgg agatggagg ctgaggtct tggccggac gggtgcaca cgtcgaggt tgcaggaag atttggaga atcttttgt cggccaaac acgacttgg aggaacagt tgaaggcgg tggtgtcgc agaggggtg aagtgaatt cacgcatac atgacttac atttgcaat ttgtgacta aaagtgggg ctagttcgc cggatggtg ataagtcca aggtgctaa taggtgata gtttgagta gaagagatt caacggcta gtcaaagga cacaaagga gaaatagtc gaagtgcat tggatctag tgcttggtg atagaatac ccaaagtta aagtgcccg attcgaaga aaattgctg ccattttga aggaaagcg atccgctgg ttctgcaga aacatctcc tctcgacca ctgtccaaa atcaggttt tatatcaac agcttgaca ccgcagcgc gagtagaaa tcggtcttg ctgataaat tagacaagg cacgcgaag aaaacgatg agcttcgat ttcagctgg aggacaaga acatagaat tagagggaa cgcgtgccc gtgttcgaa tgctggaac aactccaaa agtcgataa ccaacacct cccccgacg tcatccccg accatccca tctcccgta gcgaaatca ccacggcta gcatgaaag cgatgagtc ctctacctc tcaacctcc aaatggacc attcgagca gcaccgagt cggcccacg accaggccg agaccaacc gcaagaaca gcgacaaca gcaaacggc gccccagca aaatcccgc tgaagagct acacggcgc cgaagccac caggaggta aacacagtc ccgcacccg gtgcacgta gtcgtagcg gcgaatcgc cgggtagac cgcactctg cacagtcct ggaggaaca ggagcgagg gcaacagct tgtcgggca gcaagagca actcgtccc tgccgaaat ccaggaacg ggtcgctcg tttcggcca aggactcgc tttccagca agcttagga gtagcgact cgctttcca agctctcgt cgagtaaca atagctttg ggagtaaca ggggcacca tgaggaaga cctccacgc cggttaagt ggaccaata actccaaag acgttaccg ataaggtac gaccaaaaa ccacttcat tctggaatt tcttcaaac tacacaacg gtcagacgt aggagtgcc gccattatt atttagttg ctctcggcc gtctcgaga cgtgcagta agtgacgct tgttttttt ttatttgct ttgtccgct tatcaaatc ttgtagatc cattgctca cgtaagtag agagtagta ccttgttcc cctctccct ttttttctg aagcgtttt attcggtaa atcaatcgc atgctaatg aaatttaaa actgattca gaagcgggg tttcgtgga ctccctgct gaaatcttc gatgtgctg caaaaaacc ggtactcgt attcatttg tctgaagtc attgtgacc gagaaaaga actgtagcg aagacattt tgaaatatt agataccgt atgagaaca aaatc
- >TC000451 RB
tccatcatg cctccgttt cagagtgat catgatcag tgccttgct gttcggggg gcatcaacc tcattcttt acaaaatca tctgtttat agtgctatg tctgccatg actgatgtt tcttacatg tgtatttta taattttct attgtcgga gtgattcga tcagatgag gatctgcta cagcgtggg tggcgaggc ggtgggccc tggcatgga ctgcctcca gctgcgcca gccccccgg cccattcca ctacctgat caacgagca ggcgaagtc cctcgtggc cctccaaga gctccagca tgaagtggg cgctcttct gcagtttcg cgatctcgt catcgagac atttccaaa tctgcggac caaactcgc ctcgtcgac gcctgccac catgacttc gtcgcatca aaactccaa catcccggt ctcgattcg gacagcgga ctggacacc cggcgtccg cgtccgcaa aaaactcgc tagcaagga cgagacgcg caaaaaggg gggcgatat cgcggtgca agactccgg gttcagcac ggagacgtc gagtaagga gacgcacag cgcctcgtc cacggcacc gacggctgc agggagtcg cagttcttg cggatcctt caagtttga tgagactga agacgagct gtggaacct tctggatgt gatccaccg gaaggggac gcggttgaa ggacgaggt ggaggccct gcagggggc gatcaggga gcaggggaa catgaggga ggagaatga ttttcagag ggttttgtt tacggggtc tgctgatga tatcaggca gttgcggca tgagcggga cctcctgtt ggatcgttt ggcggagat ggaggctga ggtcttggc cggacgggt gcacacgtc gaggttgca ggaagattt ggagaatct tttgtcggc caaacacga cttggagga acagttgaa ggcggtggt gtcgcagag gggtgaagt gaattcacg catacatga cttacattt gcaatttgt gactaaaag tggggctag ttcgccgga tggtgataa gtccaaggt gctaatagg tgatagttt gagtagaag agattcaac ggctagtca aaggacaca aaggagaaa tagtcgaag tgcattgga tctagtgct tggtgatag aatacccaa agttaaagt gcccgattc gaagaaaat tgctgccat tttgaagga aagcgatcc gctggttct gcagaaaca tctcctctc gaccactgt ccaaaatca ggttttata tcaacagct tgacaccgc agcgcgagt agaaatcgg tcttgctga taaattaga caaggcacg cgaagaaaa cgatgagct tcgatttca gctggagga caagaacat agaattaga gggaacgcg tgcccgtgt tcgaatgct ggaacaact ccaaaagtc gataaccaa cacctcccc cgacgtcat ccccgacca tcccatctc ccgtagcga aatcaccac ggctagcat gaaagcgat gagtcctct acctctcaa cctccaaat ggaccattc gagcagcac cgagtcggc ccacgacca ggccgagac caaccgcaa gaacagcga caacagcaa acggcgccc cagcaaaat cccgctgaa gagctacac ggcgccgaa gccaccagg aggtaaaca cagtcccgc acccggtgc acgtagtcg tagcggcga atcgccggg tagaccgca ctctgcaca gtcctggag gaacaggag cgagggcaa cagcttgtc gggcagcaa gagcaactc gtccctgcc gaaatccag gaacgggtc gctcgtttc ggccaagga ctcgctttc cagcaagct taggagtag cgactcgct ttccaagct ctcgtcgag taacaatag ctttgggag taacagggg caccatgag gaagacctc cacgccggt taagtggac caataactc caaagacgt taccgataa ggtacgacc aaaaaccac ttcattctg gaatttctt caaactaca caacggtca gacgtagga gtgccgcca ttattattt agttgctct cggccgtct cgagacgtg cagtaagtg acgcttgtt tttttttat ttgctttgt ccgcttatc aaatcttgt agatccatt gctcacgta agtagagag tagtacctt gttcccctc tcccttttt ttctgaagc gttttattc ggtaaatca atcgcatgc taatgaaat ttaaaactg attcagaag cggggtttc gtggactcc ctgctgaaa tcttcgatg tgctgcaaa aaaccggta ctcgtattc atttgtctg aagtcattg tgaccgaga aaagaactg tagcgaaga cattttgaa atattagat accgtatga gaacaaaat c
