Status
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Gene information
General information
TC032169
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC001102
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 10966542..10984406
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032169
    iBeetle screen iB_02658

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032169 RA
      MFFQTFRHG FPFQPTAMA YDPVQRLLA IGSKCGSLR 
      ILGQPGVDI HVRHDIDGS PGSSAVLHL QFLVNEGAL 
      VSATADDSL HLWNFRQKV PQIVQSLKF QKEKITCMH 
      LPLQSKWLY VGTERGNVH VVHIETFAL SGYVINWNK 
      AIEVSRKTH PGALVHLSD NPLDASKLL IGYESGQVV 
      LWDLRNKSA EMRWQTAEL KSVAWHHEG KQFMCSHAD 
      GTLTTWSIK DRTKYLCIS QPHAKLNKD GKAEQCKPI 
      NKVEWKSSR TGEAYVIFS GGMPYDQAG RTPSITVVH 
      NKTTTVLEM ESNVIDFIT LCESFYSSE LQEPYAIIA 
      LLQNDLVVM DLQTAGFPC LENPYPMDI HESPVTCCS 
      YLADCPADL IPAFYYVGR ASGNKRQGF SEMDWPVSG 
      GSWSAQSCS YSEIILTGH ADGSIKFWD ASAGTLQVL 
      YKLKTAKIF EKQRARSLD KEEDPFAIE IISLCPESR 
      KLCVAGASS HVILFTYKK SESNDEVTV LEIPIVYEV 
      PEEGEISPD CQFSGPGSA GSDMLDFDN KKDTPTLKV 
      RAGIQKKLP GFQAQLVCL TPWNNGEPP SHITALTIN 
      SSYGLMAYG NEAGIVIID IEQKVCLLN VGSPDLYGA 
      QDPYSRVPR SPKRGGTAS DILPVNMDD RQPRSPSID 
      QMGRSPESP YSLCPYPTP VEQASEALE EDKAVPIPQ 
      AATRRKSST WKGLKRQLS RVDIKIKNP LKEKRNSIF 
      YSDVSPDPD NLDDIKSSP ESDENTVID NQSVIDLTL 
      TLGKEDQKE VYEEFPAEI QFSPGEDSD TPFVVGSLG 
      TQYEMGNSR KIGFALRPD NLELVDEDG CPVRPPRHV 
      KKKIADKRD QRLLSVPNI KFQKPEVQS LKDLRDREE 
      SVVSPQPSF TGSLMRRFS KFQLCLTLD GGPLLNLPT 
      SRTRD
    CDS sequence
    • >TC032169 RA
      atgttcttt cagacgttc cgccatggc ttccctttt 
      caacccacg gcgatggcg tacgaccct gtgcagcga 
      ttactggcg atcggttca aaatgtggc tccctcagg 
      atactcggc caaccaggc gtcgacatt cacgtccga 
      cacgacatc gatggttct ccagggtct tcagccgtc 
      cttcatttg cagtttctt gttaatgag ggcgcttta 
      gtttccgca acggcagat gactcgcta cacctctgg 
      aatttcagg cagaaagtt ccgcagatt gttcagagt 
      ctcaagttt caaaaggaa aagattacc tgcatgcat 
      ctaccatta caatctaaa tggctttac gtgggaact 
      gaacgtggc aatgtccat gtggtccac atagaaacc 
      ttcgctttg tcgggatac gtcattaac tggaacaaa 
      gcgatagaa gtttctcga aagactcat cccggcgct 
      ctagtccat ttaagtgat aacccttta gatgctagt 
      aagttattg ataggctat gaaagcggt caggttgtt 
      ctttgggat ttgaggaac aaatcggct gaaatgcga 
      tggcaaact gccgaatta aaaagtgta gcgtggcac 
      catgaaggc aaacagttc atgtgctcg catgccgat 
      ggaacatta acgacatgg agtattaaa gataggacg 
      aaatatctt tgtatatcg cagccgcat gcgaaactt 
      aacaaagat ggcaaggcg gaacaatgt aaacccata 
      aataaggtt gaatggaag tcatcaagg acgggagaa 
      gcatatgtg atattttcg ggtggaatg ccttacgac 
      caagccggc aggacacct agcataact gttgtacat 
      aataaaact accactgtg ttagaaatg gaaagtaac 
      gttattgat tttattact ttgtgcgaa tctttctat 
      agtagcgaa ctgcaggaa ccttacgca ataatagct 
      ttactacag aacgactta gtagtgatg gatttacag 
      acggctggg tttccctgt ttagagaat ccgtatccc 
      atggatatt catgaatct cccgtgacg tgttgcagt 
      tacttagcg gattgtcct gccgatcta atcccggcg 
      ttttactac gtcgggagg gcgagcggg aataaaaga 
      caaggtttc tccgagatg gactggcct gttagcggc 
      ggctcctgg tcggcgcag tcttgcagc tacagcgaa 
      attatcctc acagggcac gccgacggc agcataaag 
      ttttgggat gccagcgcc ggcacgctc caggtcctg 
      tacaagctg aaaacggcg aaaattttc gagaaacag 
      cgcgcgcgg tcgttggat aaggaggag gacccgttt 
      gccatcgaa ataatttcg ctgtgtccc gagtcgagg 
      aagctctgc gtcgccgga gcgtcgagt catgtgatt 
      ttgttcacg tataagaag tcggagagt aacgacgaa 
      gttactgtg ttggagatt cctatagtt tacgaagtt 
      cctgaggaa ggggaaatt tcgccggat tgccaattt 
      tccggaccc ggatcagcg ggatcggat atgttggat 
      ttcgacaat aaaaaagat acaccgacg cttaaggtt 
      cgtgctggt atccaaaag aaacttccc ggttttcaa 
      gcacaatta gtttgttta accccttgg aacaatgga 
      gaacctccg agccatatt actgctctt actataaat 
      agttcatac ggactgatg gcttatggt aacgaagcg 
      ggaattgtg ataatagat atagaacag aaagtttgc 
      cttttaaac gtgggcagt cccgacctg tacggtgcc 
      caagaccca tactccaga gtcccgagg agtccgaag 
      cgcggggga acggccagc gacatcctt ccggtaaac 
      atggacgac agacagccc cgtagccct agcatagac 
      cagatggga agatccccg gaaagtcct tattccttg 
      tgcccctac cccacccct gtcgaacaa gcttccgag 
      gccctagag gaggacaaa gccgtcccg ataccccaa 
      gccgccacg cgacgcaag tcctccact tggaagggt 
      cttaagaga cagctatcc agagtggat attaagatt 
      aagaacccg ttgaaagag aaaaggaac tcgatcttt 
      tactcggac gtttcgccc gatcctgac aatctagac 
      gatattaaa tcgtcgccg gaatccgat gaaaatacg 
      gtcatcgac aatcagagc gttattgat ttgacgtta 
      actcttggt aaagaggac caaaaggaa gtatacgag 
      gagtttcca gccgaaata cagttctcg ccgggggag 
      gacagcgac acgcctttc gtggtgggc tcgctcggg 
      acccagtac gaaatgggg aatagtcga aaaatcggg 
      tttgctctg cggcctgat aatttggag ctggtggac 
      gaagacgga tgtccggtg cggccacca cgccacgta 
      aagaaaaag atcgctgat aagagagac caaagactg 
      ttatctgta ccaaatatc aaatttcag aaaccagag 
      gttcagtct ctcaaagat ctaagagac agggaggag 
      agcgttgtc agcccgcag ccgtcgttt accggaagt 
      ctgatgagg cgtttcagt aagtttcaa ctctgtctc 
      accttagac ggtggcccc cttttgaac cttcccaca 
      tcccgaacc cgtgattaa
    mRNA sequence
    • >TC032169 RA
      gtgtaaata atgttcttt cagacgttc cgccatggc 
      ttccctttt caacccacg gcgatggcg tacgaccct 
      gtgcagcga ttactggcg atcggttca aaatgtggc 
      tccctcagg atactcggc caaccaggc gtcgacatt 
      cacgtccga cacgacatc gatggttct ccagggtct 
      tcagccgtc cttcatttg cagtttctt gttaatgag 
      ggcgcttta gtttccgca acggcagat gactcgcta 
      cacctctgg aatttcagg cagaaagtt ccgcagatt 
      gttcagagt ctcaagttt caaaaggaa aagattacc 
      tgcatgcat ctaccatta caatctaaa tggctttac 
      gtgggaact gaacgtggc aatgtccat gtggtccac 
      atagaaacc ttcgctttg tcgggatac gtcattaac 
      tggaacaaa gcgatagaa gtttctcga aagactcat 
      cccggcgct ctagtccat ttaagtgat aacccttta 
      gatgctagt aagttattg ataggctat gaaagcggt 
      caggttgtt ctttgggat ttgaggaac aaatcggct 
      gaaatgcga tggcaaact gccgaatta aaaagtgta 
      gcgtggcac catgaaggc aaacagttc atgtgctcg 
      catgccgat ggaacatta acgacatgg agtattaaa 
      gataggacg aaatatctt tgtatatcg cagccgcat 
      gcgaaactt aacaaagat ggcaaggcg gaacaatgt 
      aaacccata aataaggtt gaatggaag tcatcaagg 
      acgggagaa gcatatgtg atattttcg ggtggaatg 
      ccttacgac caagccggc aggacacct agcataact 
      gttgtacat aataaaact accactgtg ttagaaatg 
      gaaagtaac gttattgat tttattact ttgtgcgaa 
      tctttctat agtagcgaa ctgcaggaa ccttacgca 
      ataatagct ttactacag aacgactta gtagtgatg 
      gatttacag acggctggg tttccctgt ttagagaat 
      ccgtatccc atggatatt catgaatct cccgtgacg 
      tgttgcagt tacttagcg gattgtcct gccgatcta 
      atcccggcg ttttactac gtcgggagg gcgagcggg 
      aataaaaga caaggtttc tccgagatg gactggcct 
      gttagcggc ggctcctgg tcggcgcag tcttgcagc 
      tacagcgaa attatcctc acagggcac gccgacggc 
      agcataaag ttttgggat gccagcgcc ggcacgctc 
      caggtcctg tacaagctg aaaacggcg aaaattttc 
      gagaaacag cgcgcgcgg tcgttggat aaggaggag 
      gacccgttt gccatcgaa ataatttcg ctgtgtccc 
      gagtcgagg aagctctgc gtcgccgga gcgtcgagt 
      catgtgatt ttgttcacg tataagaag tcggagagt 
      aacgacgaa gttactgtg ttggagatt cctatagtt 
      tacgaagtt cctgaggaa ggggaaatt tcgccggat 
      tgccaattt tccggaccc ggatcagcg ggatcggat 
      atgttggat ttcgacaat aaaaaagat acaccgacg 
      cttaaggtt cgtgctggt atccaaaag aaacttccc 
      ggttttcaa gcacaatta gtttgttta accccttgg 
      aacaatgga gaacctccg agccatatt actgctctt 
      actataaat agttcatac ggactgatg gcttatggt 
      aacgaagcg ggaattgtg ataatagat atagaacag 
      aaagtttgc cttttaaac gtgggcagt cccgacctg 
      tacggtgcc caagaccca tactccaga gtcccgagg 
      agtccgaag cgcggggga acggccagc gacatcctt 
      ccggtaaac atggacgac agacagccc cgtagccct 
      agcatagac cagatggga agatccccg gaaagtcct 
      tattccttg tgcccctac cccacccct gtcgaacaa 
      gcttccgag gccctagag gaggacaaa gccgtcccg 
      ataccccaa gccgccacg cgacgcaag tcctccact 
      tggaagggt cttaagaga cagctatcc agagtggat 
      attaagatt aagaacccg ttgaaagag aaaaggaac 
      tcgatcttt tactcggac gtttcgccc gatcctgac 
      aatctagac gatattaaa tcgtcgccg gaatccgat 
      gaaaatacg gtcatcgac aatcagagc gttattgat 
      ttgacgtta actcttggt aaagaggac caaaaggaa 
      gtatacgag gagtttcca gccgaaata cagttctcg 
      ccgggggag gacagcgac acgcctttc gtggtgggc 
      tcgctcggg acccagtac gaaatgggg aatagtcga 
      aaaatcggg tttgctctg cggcctgat aatttggag 
      ctggtggac gaagacgga tgtccggtg cggccacca 
      cgccacgta aagaaaaag atcgctgat aagagagac 
      caaagactg ttatctgta ccaaatatc aaatttcag 
      aaaccagag gttcagtct ctcaaagat ctaagagac 
      agggaggag agcgttgtc agcccgcag ccgtcgttt 
      accggaagt ctgatgagg cgtttcagt aagtttcaa 
      ctctgtctc accttagac ggtggcccc cttttgaac 
      cttcccaca tcccgaacc cgtgattaa ttaacacgt 
      cgatttggt tagaaatgc ctgccttac tgactggat 
      agttttttc tctcgatat ctcttaata catattgcc 
      atttagtgg taggaatgt gtgagtagt gttagagga 
      gtaccgagt tttggtaga gttttgccg atcgagcct 
      acaccacga taccagttt gtgtttgat ttggtcgct 
      tatttgctg agttgcttt acttgtttt gttgagcag 
      tcgtcccac cgtgaacgt gtctgtcaa tttgatgac 
      taacgcttt atttcagcg ggcattggt gggtttgtg 
      actttgttc acagtggct ttggtttgt gttgaagga 
      cgaggacgg cagctgccg agattcact actgggtag 
      gagacgtag tctagccta gtcgtccct gccctttag 
      agtgcgctt agctcacgc caacctcta gcgggatgc 
      gctgctgct tgtactcgt ctagattca ctcatgctc 
      caccgttga cccgcggga tcggccgtc gaccgtatg 
      agtggagcg aataaaatc gacttgact catatttac 
      tggcacaaa ggtgcgttt tattcgcgc cgctcggcc 
      gattccgcg ccactctgt tctgatttt gtgtcggag 
      agtaagtgc tgggtttgg ggtagataa acggcgtgt 
      gcagcccgg cctcgacgt gtcccgtct ccagcacag 
      gtcgagcgc gcccagatc ctccacgtc gctctcgat 
      cgcaaggta ctcgcaaac tacacaagt gcctgtcca 
      cggctagct actcagagg actgtggca gccactttc 
      cctgtccca ctgcgccca ctgcccagc gacaacagc 
      tgatggtcg ctcgccaat attgcagtt ttggtagta 
      cagtcacca gccttcact aacaatacg tcacttatt 
      agagagatt actattcac tattcacat gctgctctc 
      ttttcctta ttctttctc gtatttttg gttcacatg 
      cgactcgac tcatgattt tctttggcg ttctcatgc 
      gattagttc cgcccagaa cacgttcca tagttcacc 
      aaaattggc tcagacaaa ttggcgcaa cagaattgt 
      tagttgttc cagttgcgg ttacacgta acttgctac 
      caagtttag ccaaata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY