Status
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Gene information
General information
TC032147
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC000532
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 10131862..10137269
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032147
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02656

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 6 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032147 RA
      MSTLPGLAS KGEKSKQKF QSLDINSLY RVSRGENLE 
      KQQQKSTFT YKHGMQSLG KVPNARRAP ANLPSLKSE 
      HSGTDASVP LVPPGGPGW GKQESTSST STTSPTTTT 
      TTASTPSSQ PSPQLSSHQ AAIALPVTT TIPPPNKHV 
      TPSATPATT DKSWSAVMS GPDSAHPPL YQSPQFQHE 
      FPSLSVGDG GPIRTGSDA QYTPGLSLR PQTEGSWIQ 
      GGSRPGAEG PPRSSSVPL GAPPQLSAQ VGLPQQQLP 
      PQFRAVMPN FMCKGSFAT GGTAGAPTQ TPPPMNGRN 
      RPENRPPPR MVDAEEITP RPIIKEEEL SRMDEIDKD 
      MGWTCHDDI DYNQKLAFS DDESTTEKN RKRN
    CDS sequence
    • >TC032147 RA
      atgtctact ttgccaggt ctggcctcc aagggggag 
      aaatcaaag caaaagttc cagtcgtta gatataaat 
      agtttatac agagttagc aggggtgaa aatttagag 
      aagcaacaa caaaaaagc acatttact tacaaacat 
      ggcatgcaa agtttggga aaggtgccc aatgcacgt 
      agggcgcca gcgaattta ccttctttg aaatcggaa 
      catagcgga actgacgca tctgtacct ttagtacca 
      ccaggcgga ccaggatgg ggaaaacaa gaatcgaca 
      tcttcaact tccaccact tctccaacg acaacaact 
      acaacagct tcaactcca agcagccaa ccttcccca 
      caattgagt tctcatcaa gctgcaatt gcccttccg 
      gtgaccaca acaatacca ccaccaaac aaacatgta 
      acaccttct gctacgcct gctaccaca gacaagtca 
      tggagtgcc gtaatgagc ggtccagat tccgcacat 
      ccaccatta tatcagagt cctcaattc caacacgag 
      ttccccagc ttgtccgtt ggagatggc ggccccata 
      cgtactggt tccgatgct cagtacacg ccagggcta 
      agcctgcgg ccccaaacc gagggctcc tggatacag 
      ggtggatct cgaccaggt gccgaaggt cccccaagg 
      agcagctcg gtccccttg ggtgccccg ccacagctg 
      tcggcccag gtcggcctc cctcaacaa caattgcca 
      ccgcaattt cgggcggtc atgccaaat tttatgtgc 
      aaaggtagc ttcgctact ggtggaact gctggtgct 
      cctactcaa actcctccg ccgatgaat ggacgtaat 
      cggccagaa aatcgcccg ccaccgcga atggtggac 
      gcggaggaa attacccca agaccgatc attaaggaa 
      gaagaattg agtcgcatg gatgaaatt gacaaggac 
      atgggatgg acctgtcat gatgatatt gattacaac 
      cagaaattg gctttcagt gatgacgaa tctaccacg 
      gagaaaaac cgaaaaaga aactaa
    mRNA sequence
    • >TC032147 RA
      ccagggtaa taaatatac gtgacgtca tgtattgat 
      taaaactgc cgtagtcgg cgtacgcca ttttgactt 
      aagtgaaaa agtttacgt ttagtggta aaaacaagc 
      tgaaatctg tgatcatcc gtccctatt ttcgtttaa 
      tcggatcta tttcttgtt gattactgt ttacccatc 
      tggacggac caaggaaaa aggttcgtt cgctgggtc 
      ctgaagatc taaatggtt gcgtgcaga cagcatgtc 
      tactttgcc aggtctggc ctccaaggg ggagaaatc 
      aaagcaaaa gttccagtc gttagatat aaatagttt 
      atacagagt tagcagggg tgaaaattt agagaagca 
      acaacaaaa aagcacatt tacttacaa acatggcat 
      gcaaagttt gggaaaggt gcccaatgc acgtagggc 
      gccagcgaa tttaccttc tttgaaatc ggaacatag 
      cggaactga cgcatctgt acctttagt accaccagg 
      cggaccagg atggggaaa acaagaatc gacatcttc 
      aacttccac cacttctcc aacgacaac aactacaac 
      agcttcaac tccaagcag ccaaccttc cccacaatt 
      gagttctca tcaagctgc aattgccct tccggtgac 
      cacaacaat accaccacc aaacaaaca tgtaacacc 
      ttctgctac gcctgctac cacagacaa gtcatggag 
      tgccgtaat gagcggtcc agattccgc acatccacc 
      attatatca gagtcctca attccaaca cgagttccc 
      cagcttgtc cgttggaga tggcggccc catacgtac 
      tggttccga tgctcagta cacgccagg gctaagcct 
      gcggcccca aaccgaggg ctcctggat acagggtgg 
      atctcgacc aggtgccga aggtccccc aaggagcag 
      ctcggtccc cttgggtgc cccgccaca gctgtcggc 
      ccaggtcgg cctccctca acaacaatt gccaccgca 
      atttcgggc ggtcatgcc aaattttat gtgcaaagg 
      tagcttcgc tactggtgg aactgctgg tgctcctac 
      tcaaactcc tccgccgat gaatggacg taatcggcc 
      agaaaatcg cccgccacc gcgaatggt ggacgcgga 
      ggaaattac cccaagacc gatcattaa ggaagaaga 
      attgagtcg catggatga aattgacaa ggacatggg 
      atggacctg tcatgatga tattgatta caaccagaa 
      attggcttt cagtgatga cgaatctac cacggagaa 
      aaaccgaaa aagaaacta aaactaccc caactacta 
      cacccaata cgaccactc aaaagacca cgaaaagtc 
      tgactctca tccgaatga atctcagtc ccgtaacaa 
      ttggaacaa taaacgttc ttcggatga ggaactttg 
      gaagcaacg tcgccaaca caacaagga tctagaatt 
      aaccgttca gagagccaa gcaacgcaa agaagaaga 
      ggaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY