| General information | |||
| TC001035 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 10011505..10017579 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC001035 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02655 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Fertility
mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: -
Number of animals showing the phenotype: 1
mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 3
mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 3
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
Adult morphology
pronotum surface irregular -
Number of animals showing the phenotype: 9
abdomen tergum surface irregular - Number of animals showing the phenotype: 7
| Comment: number of sensory organs decreased |
abdomen tergum surface irregular - Number of animals showing the phenotype: 7
| Comment: number of sensory organs slightly decreased |
Fertility
egg production: number no/almost no eggs -
vitellogenic egg chamber late not present - Number of animals showing the phenotype: 4
vitellogenic egg chamber potentially degenerated - Number of animals showing the phenotype: 4
yolk partially structure irregular - Number of animals showing the phenotype: 4
fertility after larval injection decreased (no living offspring) -
vitellogenic egg chamber late not present - Number of animals showing the phenotype: 4
vitellogenic egg chamber potentially degenerated - Number of animals showing the phenotype: 4
yolk partially structure irregular - Number of animals showing the phenotype: 4
fertility after larval injection decreased (no living offspring) -
- >TC001035 RA
MDVLNAVYN LNNEETLKS SWFGEVFSS FSTQETYQW GFQLLPVLL AAIFATIIS VTYFCIRKC HRKALFARQ LKSEEASAR FRKRDKALF YGRKMLRKV KNISGQVRN SGQGRKRKM VMKFARRIL QLKKENDTE QLKVLEPPV EYLQEEMLS DDRMPPDAL YMLQSIRVF GHFEKPIFL KLCKHTEVI NVDAGQHLF KIGDADENV YIVQSGKLN VFIASADNT NLSLKIVKP GESVTSLLS FTDVLTGNT NPYKTVSAK AIEDSTVVK LPMVAFQEI FHEYPDIFI RVIQVIMVR LQRVTFTAL HQYLGLSAE LVKQGPKSR NSKNPLVGG SPMKKKRED FRDGSFSSM DASQPIPVP GHRRSKSSL ESKSFSPQT PDMVSDAEM ATSGMTPDV ALLQRKRHS VDNVDEDTL IEIATEAFI KELNLEDDT MLKGNVQIK EIAAGTYLM QEDSNKDVA LCYVISGAL IVSQKMTES EDEVHMFTS YHGEFVGGL AVLTGEPSF FTIRAKHFT RIALLSKNT FYSMMKDHP NIVLYVAHS VVQRLSPFV RQVDFALDW LFMESGRAV YRQDDESDS TYIVLSGRL RSVITHKNG KKELVGEYG KGDLIGVVE MVTHTKRST TVIAVRDSE LAKLPEGLF NAIKLKYPI VVTRLINLL GHRILGSWK KPTISVHAP SSSAIDSRP SQLNFSTVA IVAASDDVP LTAFTYELY HCLCAIGPT LRLTSDIIR KILGPTIMD PNNEYRLTS WLAQQEDQH KISLYQCDL TVSAWTQRC IRQADCILI VGLGENHPS IGKVEKEVE RLAMRTQKE LVLLHREGG KPHNTVAWI NMRSWVAWH HHILSPNRM FSRKSLYRI NELYSKVCQ SEPNIHSDF SRLARWLTG KSVGLVLGG GGARGSAHV GMIKAIQEA GIPIDMVGG VSIGAFMGA LWCRERNIT TTTQKAREW SKKMTQWWR QILDLTYPM TSMFSGRDF NQTIKGTFG DTYIEDLWL PYFTVTTDI TASCMRLHS HGSLWRYVR ASMSLSGYM PPLCDPVDG HLLLDGGYT NNLPADVMR SLGANHIIA IDVGSVDDQ DLTNYGDDL SGWWLLWKR WNPFTDPVK VPNLPDIQS RLAYVSCVR QLEEVKNSD YCEYVRPPI DKYKTLQFG SFDEIREVG YQHGKAYFE EKSKSGNLP IFKAPTVTN KPETEHILS DYTFTDLAQ MVCKVSRPY EESSSSSSS GDEFEDGQD GYASEPTVC LMDNKIDKL LHMRRAGGS LSLSENEIE SEVEFDVTN RKSEKELKF SSL
- >TC001035 RB
MDVLNAVYN LNNEETLKS SWFGEVFSS FSTQETYQW GFQLLPVLL AAIFATIIS VTYFCIRKC HRKALFARQ LKSEEASAR FRKRDKALF YGRKMLRKV KNISGQVRN SGQGRKRKM VMKFARRIL QLKKENDTE QLKVLEPPV EYLQEEMLS DDRMPPDAL YMLQSIRVF GHFEKPIFL KLCKHTEVI NVDAGQHLF KIGDADENV YIVQSGKLN VFIASADNT NLSLKIVKP GESVTSLLS FTDVLTGNT NPYKTVSAK AIEDSTVVK LPMVAFQEI FHEYPDIFI RVIQVIMVR LQRVTFTAL HQYLGLSAE LVKQGPKSR NSKNPLVGG SPMKKKRED FRDGSFSSM DASQPIPVP GHRRSKSSL ESKSFSPQT PDMVSDAEM ATSGMTPDV ALLQRKRHS VDNVDEDTL IEIATEAFI KELNLEDDT MLKGNVQIK EIAAGTYLM QEDSNKDVA LCYVISGAL IVSQKMTES EDEVHMFTS YHGEFVGGL AVLTGEPSF FTIRAKHFT RIALLSKNT FYSMMKDHP NIVLYVAHS VVQRLSPFV RQVDFALDW LFMESGRAV YRQDDESDS TYIVLSGRL RSVITHKNG KKELVGEYG KGDLIGVVE MVTHTKRST TVIAVRDSE LAKLPEGLF NAIKLKYPI VVTRLINLL GHRILGSWK KPTISVHAP SSSAIDSRP SQLNFSTVA IVAASDDVP LTAFTYELY HCLCAIGPT LRLTSDIIR KILGPTIMD PNNEYRLTS WLAQQEDQH KISLYQCDL TVSAWTQRC IRQADCILI VGLGENHPS IGKVEKEVE RLAMRTQKE LVLLHREGG KPHNTVAWI NMRSWVAWH HHILSPNRM FSRKSLYRI NELYSKVCQ SEPNIHSDF SRLARWLTG KSVGLVLGG GGARGSAHV GMIKAIQEA GIPIDMVGG VSIGAFMGA LWCRERNIT TTTQKAREW SKKMTQWWR QILDLTYPM TSMFSGRDF NQTIKGTFG DTYIEDLWL PYFTVTTDI TASCMRLHS HGSLWRYVR ASMSLSGYM PPLCDPVDG HLLLDGGYT NNLPGKKPK NTNSVVCMP SVTGKYTGS VRLHTQNQG HLWRYVRAS MSIVGIFPP LCDPLDGHL LADGCYVNN VPADVMRSL GANHIIAID VGSVDDQDL TNYGDDLSG WWLLWKRWN PFTDPVKVP NLPDIQSRL AYVSCVRQL EEVKNSDYC EYVRPPIDK YKTLQFGSF DEIREVGYQ HGKAYFEEK SKSGNLPIF KAPTVTNKP ETEHILSDY TFTDLAQMV CKVSRPYEE SSSSSSSGD EFEDGQDGY ASEPTVCLM DNKIDKLLH MRRAGGSLS LSENEIESE VEFDVTNRK SEKELKFSS L
- >TC001035 RA
atggacgta ttaaatgcg gtatacaac ttaaacaat gaagaaacc ctcaagtcc agttggttc ggtgaggtc ttctcgagt ttttcgact caagagacg taccagtgg ggcttccag ctactgccc gtgttactg gcagcgatt ttcgcaacg ataatttca gtaacgtat ttttgcata cgaaaatgc caccgaaaa gctcttttt gcgcgacaa ttgaaatcc gaagaggca agtgcaagg ttcaggaag agggataaa gcgttgttt tacgggcgc aaaatgtta cgcaaggtg aaaaatatt tcgggccaa gtgcggaac tccggtcag ggcaggaaa aggaaaatg gtgatgaaa ttcgcgaga cggatttta caactaaag aaggaaaat gatacggaa caattgaag gttctggaa ccgcctgtt gaatattta caagaggaa atgttgagc gatgatcgg atgccgccg gatgcgctg tacatgctc cagagcatt agggtgttt ggccacttt gagaaaccg attttcttg aagttgtgc aagcacacg gaagttatt aacgtagac gccgggcaa catttattc aagataggt gatgccgac gaaaacgtg tacatcgtg caaagcggc aaactcaat gttttcatc gcgtcggcc gataacacg aacctctcc ctcaaaatc gtaaagccg ggcgaatct gtgacctcc ctcctcagt ttcaccgat gtcttaact ggcaacact aatccgtac aaaaccgtt tcggccaaa gcgatcgaa gactcaacc gttgtcaag ctgccaatg gtcgcgttt caagaaatc ttccacgaa tatcctgat attttcatt cgcgtaatt caagtcatt atggtgcgt ttgcaacgc gtcaccttc acagcgttg catcagtat ctcggtctg agcgccgaa ctagtcaaa caggggcca aagagtagg aatagcaaa aatccacta gtcgggggt tcaccaatg aaaaagaaa cgtgaagac tttagggat gggtcgttt agttcgatg gatgcgtcg caaccgata ccggtaccg gggcacagg aggagtaaa tcgagcctt gagtcgaag tctttctcg ccacaaacg ccggatatg gtcagcgat gccgaaatg gccacttcc ggcatgaca cccgacgtt gcactttta cagcgaaaa agacactcg gttgataat gtcgatgag gacactcta atcgaaatc gcgactgag gctttcatc aaagaactg aaccttgaa gatgacact atgttgaaa ggaaacgtc caaatcaag gaaatagca gccggtact tatctcatg caggaggat tcaaataaa gacgttgct ttgtgctat gtcatttcg ggcgccttg attgtttcg cagaaaatg acagaaagt gaggatgaa gtccacatg ttcacctcc tatcacggc gaattcgtc ggcggtctc gccgtacta accggcgaa ccgagtttt ttcacaatt cgggcgaaa cacttcacc cgaattgcc ctactgtcg aaaaacaca ttttacagt atgatgaaa gaccacccc aatatcgta ctttacgtg gcccattcg gtcgttcag cggctttcg ccgttcgtg cgtcaagtc gatttcgct cttgattgg ttgtttatg gagtcgggc cgcgccgtc taccgacaa gacgacgaa agcgactcg acttacatc gttctatcg ggtcgtctg cgatcggtt ataacccac aagaacggc aagaaggag ttggtgggg gaatacggt aaaggcgac ttgattggc gtcgtcgaa atggtgact cacacgaag cgaagtacc acggttata gcagttaga gactccgag ttggctaaa ctacccgaa ggattgttc aatgcaatt aaattgaaa tacccgatt gttgtcaca agactgatc aatttactg gggcacagg attttaggt tcgtggaaa aaaccgacg attagcgtg catgcgcct agtagttcg gcgattgat agccgaccc tcgcagcta aatttttca actgttgcg atcgtggcc gcctctgat gatgtacct ctcacagct ttcacttac gaactgtat cactgttta tgtgccatt ggaccaact ttaagactg acttccgat ataattcgt aagattctg ggcccgacc atcatggat ccgaataat gagtatcgg ttaacatcg tggttggcc cagcaggag gaccagcat aaaatctcg ctgtatcag tgcgacttg accgttagt gcatggacg cagcgatgt attcggcaa gctgactgt attctgata gtcggtttg ggcgaaaat cacccgagt ataggcaaa gtcgagaaa gaagtcgaa cgactagcc atgcggacg caaaaagaa ctagttttg ttgcatcgt gagggtggc aagcctcac aatacagtc gcctggatc aacatgagg agctgggtg gcgtggcat caccacatt ttgagccct aataggatg ttttcaaga aaatctcta tataggata aacgagctg tactcgaag gtgtgccag tccgagccg aacatccat tcggacttt tcccggttg gctcgttgg ctaaccggc aaatcagtc ggtttggtt ttgggcggg ggcggagct cggggttct gcacatgtc ggaatgatt aaagcaatc caggaggct gggatcccg attgacatg gtgggtggg gtcagcatt ggggctttc atgggggcg ttgtggtgt cgcgagcgg aatattacc acgacaacg cagaaagcg agagaatgg tccaagaaa atgacccaa tggtggcgc caaatcctc gatttgacc tatccgatg acgtcgatg ttcagcggt cgtgatttt aatcaaaca atcaagggc acttttggt gacacgtat attgaggac ttgtggtta ccgtatttt acggttaca accgatata accgcctcg tgtatgcgg ttgcattcg cacggctct ctgtggcgg tacgttcgt gcctctatg tctctatcc ggatacatg ccgcctctg tgtgaccct gtcgatggc caccttctt ctagacgga ggctacact aacaatctt ccagctgac gtcatgcgg agtttgggg gccaatcac ataatcgcg attgatgtg ggtagtgtg gatgaccaa gatttgacc aattacggg gatgatttg tcggggtgg tggttgttg tggaaacgg tggaatccc ttcactgat cccgtcaaa gtacccaat ttacccgat attcaatca aggctggct tatgtcagc tgtgtaaga cagttggaa gaagtaaaa aacagtgat tattgtgaa tacgtccgt ccacctatc gataagtat aaaacgcta caattcgga agttttgat gaaattcgt gaagtcggt taccaacat ggcaaagct tactttgag gaaaagtca aaatcgggt aatttaccc atcttcaaa gcgccaact gttaccaat aaacccgaa acggaacat attttgtcc gattatact ttcaccgat ttagcacaa atggtttgc aaagtctca agaccttat gaagagtca tcaagttcg agttcttca ggcgatgag tttgaagat ggccaggac ggttacgca tctgaacct acagtgtgt ctaatggat aataaaatt gataaactc ctacatatg agaagagcc ggcggttct ctttcatta tccgaaaat gaaatagaa tccgaagtt gagttcgat gtaacaaat aggaaaagt gaaaaagaa ttgaaattc tctagtctt tag
- >TC001035 RB
atggacgta ttaaatgcg gtatacaac ttaaacaat gaagaaacc ctcaagtcc agttggttc ggtgaggtc ttctcgagt ttttcgact caagagacg taccagtgg ggcttccag ctactgccc gtgttactg gcagcgatt ttcgcaacg ataatttca gtaacgtat ttttgcata cgaaaatgc caccgaaaa gctcttttt gcgcgacaa ttgaaatcc gaagaggca agtgcaagg ttcaggaag agggataaa gcgttgttt tacgggcgc aaaatgtta cgcaaggtg aaaaatatt tcgggccaa gtgcggaac tccggtcag ggcaggaaa aggaaaatg gtgatgaaa ttcgcgaga cggatttta caactaaag aaggaaaat gatacggaa caattgaag gttctggaa ccgcctgtt gaatattta caagaggaa atgttgagc gatgatcgg atgccgccg gatgcgctg tacatgctc cagagcatt agggtgttt ggccacttt gagaaaccg attttcttg aagttgtgc aagcacacg gaagttatt aacgtagac gccgggcaa catttattc aagataggt gatgccgac gaaaacgtg tacatcgtg caaagcggc aaactcaat gttttcatc gcgtcggcc gataacacg aacctctcc ctcaaaatc gtaaagccg ggcgaatct gtgacctcc ctcctcagt ttcaccgat gtcttaact ggcaacact aatccgtac aaaaccgtt tcggccaaa gcgatcgaa gactcaacc gttgtcaag ctgccaatg gtcgcgttt caagaaatc ttccacgaa tatcctgat attttcatt cgcgtaatt caagtcatt atggtgcgt ttgcaacgc gtcaccttc acagcgttg catcagtat ctcggtctg agcgccgaa ctagtcaaa caggggcca aagagtagg aatagcaaa aatccacta gtcgggggt tcaccaatg aaaaagaaa cgtgaagac tttagggat gggtcgttt agttcgatg gatgcgtcg caaccgata ccggtaccg gggcacagg aggagtaaa tcgagcctt gagtcgaag tctttctcg ccacaaacg ccggatatg gtcagcgat gccgaaatg gccacttcc ggcatgaca cccgacgtt gcactttta cagcgaaaa agacactcg gttgataat gtcgatgag gacactcta atcgaaatc gcgactgag gctttcatc aaagaactg aaccttgaa gatgacact atgttgaaa ggaaacgtc caaatcaag gaaatagca gccggtact tatctcatg caggaggat tcaaataaa gacgttgct ttgtgctat gtcatttcg ggcgccttg attgtttcg cagaaaatg acagaaagt gaggatgaa gtccacatg ttcacctcc tatcacggc gaattcgtc ggcggtctc gccgtacta accggcgaa ccgagtttt ttcacaatt cgggcgaaa cacttcacc cgaattgcc ctactgtcg aaaaacaca ttttacagt atgatgaaa gaccacccc aatatcgta ctttacgtg gcccattcg gtcgttcag cggctttcg ccgttcgtg cgtcaagtc gatttcgct cttgattgg ttgtttatg gagtcgggc cgcgccgtc taccgacaa gacgacgaa agcgactcg acttacatc gttctatcg ggtcgtctg cgatcggtt ataacccac aagaacggc aagaaggag ttggtgggg gaatacggt aaaggcgac ttgattggc gtcgtcgaa atggtgact cacacgaag cgaagtacc acggttata gcagttaga gactccgag ttggctaaa ctacccgaa ggattgttc aatgcaatt aaattgaaa tacccgatt gttgtcaca agactgatc aatttactg gggcacagg attttaggt tcgtggaaa aaaccgacg attagcgtg catgcgcct agtagttcg gcgattgat agccgaccc tcgcagcta aatttttca actgttgcg atcgtggcc gcctctgat gatgtacct ctcacagct ttcacttac gaactgtat cactgttta tgtgccatt ggaccaact ttaagactg acttccgat ataattcgt aagattctg ggcccgacc atcatggat ccgaataat gagtatcgg ttaacatcg tggttggcc cagcaggag gaccagcat aaaatctcg ctgtatcag tgcgacttg accgttagt gcatggacg cagcgatgt attcggcaa gctgactgt attctgata gtcggtttg ggcgaaaat cacccgagt ataggcaaa gtcgagaaa gaagtcgaa cgactagcc atgcggacg caaaaagaa ctagttttg ttgcatcgt gagggtggc aagcctcac aatacagtc gcctggatc aacatgagg agctgggtg gcgtggcat caccacatt ttgagccct aataggatg ttttcaaga aaatctcta tataggata aacgagctg tactcgaag gtgtgccag tccgagccg aacatccat tcggacttt tcccggttg gctcgttgg ctaaccggc aaatcagtc ggtttggtt ttgggcggg ggcggagct cggggttct gcacatgtc ggaatgatt aaagcaatc caggaggct gggatcccg attgacatg gtgggtggg gtcagcatt ggggctttc atgggggcg ttgtggtgt cgcgagcgg aatattacc acgacaacg cagaaagcg agagaatgg tccaagaaa atgacccaa tggtggcgc caaatcctc gatttgacc tatccgatg acgtcgatg ttcagcggt cgtgatttt aatcaaaca atcaagggc acttttggt gacacgtat attgaggac ttgtggtta ccgtatttt acggttaca accgatata accgcctcg tgtatgcgg ttgcattcg cacggctct ctgtggcgg tacgttcgt gcctctatg tctctatcc ggatacatg ccgcctctg tgtgaccct gtcgatggc caccttctt ctagacgga ggctacact aacaatctt ccaggtaaa aaaccaaaa aacactaac tcagtcgtt tgcatgcct tctgtcacc ggtaagtac actggctcg gttcggtta cacacacaa aaccaaggt catttgtgg agatatgtg agggccagt atgtcaatt gttggcatt tttccgcca ctttgtgat cctttagat gggcatttg ttagctgat gggtgttac gtcaacaat gtaccagct gacgtcatg cggagtttg ggggccaat cacataatc gcgattgat gtgggtagt gtggatgac caagatttg accaattac ggggatgat ttgtcgggg tggtggttg ttgtggaaa cggtggaat cccttcact gatcccgtc aaagtaccc aatttaccc gatattcaa tcaaggctg gcttatgtc agctgtgta agacagttg gaagaagta aaaaacagt gattattgt gaatacgtc cgtccacct atcgataag tataaaacg ctacaattc ggaagtttt gatgaaatt cgtgaagtc ggttaccaa catggcaaa gcttacttt gaggaaaag tcaaaatcg ggtaattta cccatcttc aaagcgcca actgttacc aataaaccc gaaacggaa catattttg tccgattat actttcacc gatttagca caaatggtt tgcaaagtc tcaagacct tatgaagag tcatcaagt tcgagttct tcaggcgat gagtttgaa gatggccag gacggttac gcatctgaa cctacagtg tgtctaatg gataataaa attgataaa ctcctacat atgagaaga gccggcggt tctctttca ttatccgaa aatgaaata gaatccgaa gttgagttc gatgtaaca aataggaaa agtgaaaaa gaattgaaa ttctctagt ctttag
- >TC001035 RA
aaattcatc caaataggt ttgtgattt tattcaacc tttaatcac taattcttt aatcatcag agtctaatt tctataatg tgcgagcta aaatggacg tattaaatg cggtataca acttaaaca atgaagaaa ccctcaagt ccagttggt tcggtgagg tcttctcga gtttttcga ctcaagaga cgtaccagt ggggcttcc agctactgc ccgtgttac tggcagcga ttttcgcaa cgataattt cagtaacgt atttttgca tacgaaaat gccaccgaa aagctcttt ttgcgcgac aattgaaat ccgaagagg caagtgcaa ggttcagga agagggata aagcgttgt tttacgggc gcaaaatgt tacgcaagg tgaaaaata tttcgggcc aagtgcgga actccggtc agggcagga aaaggaaaa tggtgatga aattcgcga gacggattt tacaactaa agaaggaaa atgatacgg aacaattga aggttctgg aaccgcctg ttgaatatt tacaagagg aaatgttga gcgatgatc ggatgccgc cggatgcgc tgtacatgc tccagagca ttagggtgt ttggccact ttgagaaac cgattttct tgaagttgt gcaagcaca cggaagtta ttaacgtag acgccgggc aacatttat tcaagatag gtgatgccg acgaaaacg tgtacatcg tgcaaagcg gcaaactca atgttttca tcgcgtcgg ccgataaca cgaacctct ccctcaaaa tcgtaaagc cgggcgaat ctgtgacct ccctcctca gtttcaccg atgtcttaa ctggcaaca ctaatccgt acaaaaccg tttcggcca aagcgatcg aagactcaa ccgttgtca agctgccaa tggtcgcgt ttcaagaaa tcttccacg aatatcctg atattttca ttcgcgtaa ttcaagtca ttatggtgc gtttgcaac gcgtcacct tcacagcgt tgcatcagt atctcggtc tgagcgccg aactagtca aacaggggc caaagagta ggaatagca aaaatccac tagtcgggg gttcaccaa tgaaaaaga aacgtgaag actttaggg atgggtcgt ttagttcga tggatgcgt cgcaaccga taccggtac cggggcaca ggaggagta aatcgagcc ttgagtcga agtctttct cgccacaaa cgccggata tggtcagcg atgccgaaa tggccactt ccggcatga cacccgacg ttgcacttt tacagcgaa aaagacact cggttgata atgtcgatg aggacactc taatcgaaa tcgcgactg aggctttca tcaaagaac tgaaccttg aagatgaca ctatgttga aaggaaacg tccaaatca aggaaatag cagccggta cttatctca tgcaggagg attcaaata aagacgttg ctttgtgct atgtcattt cgggcgcct tgattgttt cgcagaaaa tgacagaaa gtgaggatg aagtccaca tgttcacct cctatcacg gcgaattcg tcggcggtc tcgccgtac taaccggcg aaccgagtt ttttcacaa ttcgggcga aacacttca cccgaattg ccctactgt cgaaaaaca cattttaca gtatgatga aagaccacc ccaatatcg tactttacg tggcccatt cggtcgttc agcggcttt cgccgttcg tgcgtcaag tcgatttcg ctcttgatt ggttgttta tggagtcgg gccgcgccg tctaccgac aagacgacg aaagcgact cgacttaca tcgttctat cgggtcgtc tgcgatcgg ttataaccc acaagaacg gcaagaagg agttggtgg gggaatacg gtaaaggcg acttgattg gcgtcgtcg aaatggtga ctcacacga agcgaagta ccacggtta tagcagtta gagactccg agttggcta aactacccg aaggattgt tcaatgcaa ttaaattga aatacccga ttgttgtca caagactga tcaatttac tggggcaca ggattttag gttcgtgga aaaaaccga cgattagcg tgcatgcgc ctagtagtt cggcgattg atagccgac cctcgcagc taaattttt caactgttg cgatcgtgg ccgcctctg atgatgtac ctctcacag ctttcactt acgaactgt atcactgtt tatgtgcca ttggaccaa ctttaagac tgacttccg atataattc gtaagattc tgggcccga ccatcatgg atccgaata atgagtatc ggttaacat cgtggttgg cccagcagg aggaccagc ataaaatct cgctgtatc agtgcgact tgaccgtta gtgcatgga cgcagcgat gtattcggc aagctgact gtattctga tagtcggtt tgggcgaaa atcacccga gtataggca aagtcgaga aagaagtcg aacgactag ccatgcgga cgcaaaaag aactagttt tgttgcatc gtgagggtg gcaagcctc acaatacag tcgcctgga tcaacatga ggagctggg tggcgtggc atcaccaca ttttgagcc ctaatagga tgttttcaa gaaaatctc tatatagga taaacgagc tgtactcga aggtgtgcc agtccgagc cgaacatcc attcggact tttcccggt tggctcgtt ggctaaccg gcaaatcag tcggtttgg ttttgggcg ggggcggag ctcggggtt ctgcacatg tcggaatga ttaaagcaa tccaggagg ctgggatcc cgattgaca tggtgggtg gggtcagca ttggggctt tcatggggg cgttgtggt gtcgcgagc ggaatatta ccacgacaa cgcagaaag cgagagaat ggtccaaga aaatgaccc aatggtggc gccaaatcc tcgatttga cctatccga tgacgtcga tgttcagcg gtcgtgatt ttaatcaaa caatcaagg gcacttttg gtgacacgt atattgagg acttgtggt taccgtatt ttacggtta caaccgata taaccgcct cgtgtatgc ggttgcatt cgcacggct ctctgtggc ggtacgttc gtgcctcta tgtctctat ccggataca tgccgcctc tgtgtgacc ctgtcgatg gccaccttc ttctagacg gaggctaca ctaacaatc ttccagctg acgtcatgc ggagtttgg gggccaatc acataatcg cgattgatg tgggtagtg tggatgacc aagatttga ccaattacg gggatgatt tgtcggggt ggtggttgt tgtggaaac ggtggaatc ccttcactg atcccgtca aagtaccca atttacccg atattcaat caaggctgg cttatgtca gctgtgtaa gacagttgg aagaagtaa aaaacagtg attattgtg aatacgtcc gtccaccta tcgataagt ataaaacgc tacaattcg gaagttttg atgaaattc gtgaagtcg gttaccaac atggcaaag cttactttg aggaaaagt caaaatcgg gtaatttac ccatcttca aagcgccaa ctgttacca ataaacccg aaacggaac atattttgt ccgattata ctttcaccg atttagcac aaatggttt gcaaagtct caagacctt atgaagagt catcaagtt cgagttctt caggcgatg agtttgaag atggccagg acggttacg catctgaac ctacagtgt gtctaatgg ataataaaa ttgataaac tcctacata tgagaagag ccggcggtt ctctttcat tatccgaaa atgaaatag aatccgaag ttgagttcg atgtaacaa ataggaaaa gtgaaaaag aattgaaat tctctagtc tttagtact ttagtagtt aagtttgtt tccctttgt tatcagtta a
- >TC001035 RB
aaattcatc caaataggt ttgtgattt tattcaacc tttaatcac taattcttt aatcatcag agtctaatt tctataatg tgcgagcta aaatggacg tattaaatg cggtataca acttaaaca atgaagaaa ccctcaagt ccagttggt tcggtgagg tcttctcga gtttttcga ctcaagaga cgtaccagt ggggcttcc agctactgc ccgtgttac tggcagcga ttttcgcaa cgataattt cagtaacgt atttttgca tacgaaaat gccaccgaa aagctcttt ttgcgcgac aattgaaat ccgaagagg caagtgcaa ggttcagga agagggata aagcgttgt tttacgggc gcaaaatgt tacgcaagg tgaaaaata tttcgggcc aagtgcgga actccggtc agggcagga aaaggaaaa tggtgatga aattcgcga gacggattt tacaactaa agaaggaaa atgatacgg aacaattga aggttctgg aaccgcctg ttgaatatt tacaagagg aaatgttga gcgatgatc ggatgccgc cggatgcgc tgtacatgc tccagagca ttagggtgt ttggccact ttgagaaac cgattttct tgaagttgt gcaagcaca cggaagtta ttaacgtag acgccgggc aacatttat tcaagatag gtgatgccg acgaaaacg tgtacatcg tgcaaagcg gcaaactca atgttttca tcgcgtcgg ccgataaca cgaacctct ccctcaaaa tcgtaaagc cgggcgaat ctgtgacct ccctcctca gtttcaccg atgtcttaa ctggcaaca ctaatccgt acaaaaccg tttcggcca aagcgatcg aagactcaa ccgttgtca agctgccaa tggtcgcgt ttcaagaaa tcttccacg aatatcctg atattttca ttcgcgtaa ttcaagtca ttatggtgc gtttgcaac gcgtcacct tcacagcgt tgcatcagt atctcggtc tgagcgccg aactagtca aacaggggc caaagagta ggaatagca aaaatccac tagtcgggg gttcaccaa tgaaaaaga aacgtgaag actttaggg atgggtcgt ttagttcga tggatgcgt cgcaaccga taccggtac cggggcaca ggaggagta aatcgagcc ttgagtcga agtctttct cgccacaaa cgccggata tggtcagcg atgccgaaa tggccactt ccggcatga cacccgacg ttgcacttt tacagcgaa aaagacact cggttgata atgtcgatg aggacactc taatcgaaa tcgcgactg aggctttca tcaaagaac tgaaccttg aagatgaca ctatgttga aaggaaacg tccaaatca aggaaatag cagccggta cttatctca tgcaggagg attcaaata aagacgttg ctttgtgct atgtcattt cgggcgcct tgattgttt cgcagaaaa tgacagaaa gtgaggatg aagtccaca tgttcacct cctatcacg gcgaattcg tcggcggtc tcgccgtac taaccggcg aaccgagtt ttttcacaa ttcgggcga aacacttca cccgaattg ccctactgt cgaaaaaca cattttaca gtatgatga aagaccacc ccaatatcg tactttacg tggcccatt cggtcgttc agcggcttt cgccgttcg tgcgtcaag tcgatttcg ctcttgatt ggttgttta tggagtcgg gccgcgccg tctaccgac aagacgacg aaagcgact cgacttaca tcgttctat cgggtcgtc tgcgatcgg ttataaccc acaagaacg gcaagaagg agttggtgg gggaatacg gtaaaggcg acttgattg gcgtcgtcg aaatggtga ctcacacga agcgaagta ccacggtta tagcagtta gagactccg agttggcta aactacccg aaggattgt tcaatgcaa ttaaattga aatacccga ttgttgtca caagactga tcaatttac tggggcaca ggattttag gttcgtgga aaaaaccga cgattagcg tgcatgcgc ctagtagtt cggcgattg atagccgac cctcgcagc taaattttt caactgttg cgatcgtgg ccgcctctg atgatgtac ctctcacag ctttcactt acgaactgt atcactgtt tatgtgcca ttggaccaa ctttaagac tgacttccg atataattc gtaagattc tgggcccga ccatcatgg atccgaata atgagtatc ggttaacat cgtggttgg cccagcagg aggaccagc ataaaatct cgctgtatc agtgcgact tgaccgtta gtgcatgga cgcagcgat gtattcggc aagctgact gtattctga tagtcggtt tgggcgaaa atcacccga gtataggca aagtcgaga aagaagtcg aacgactag ccatgcgga cgcaaaaag aactagttt tgttgcatc gtgagggtg gcaagcctc acaatacag tcgcctgga tcaacatga ggagctggg tggcgtggc atcaccaca ttttgagcc ctaatagga tgttttcaa gaaaatctc tatatagga taaacgagc tgtactcga aggtgtgcc agtccgagc cgaacatcc attcggact tttcccggt tggctcgtt ggctaaccg gcaaatcag tcggtttgg ttttgggcg ggggcggag ctcggggtt ctgcacatg tcggaatga ttaaagcaa tccaggagg ctgggatcc cgattgaca tggtgggtg gggtcagca ttggggctt tcatggggg cgttgtggt gtcgcgagc ggaatatta ccacgacaa cgcagaaag cgagagaat ggtccaaga aaatgaccc aatggtggc gccaaatcc tcgatttga cctatccga tgacgtcga tgttcagcg gtcgtgatt ttaatcaaa caatcaagg gcacttttg gtgacacgt atattgagg acttgtggt taccgtatt ttacggtta caaccgata taaccgcct cgtgtatgc ggttgcatt cgcacggct ctctgtggc ggtacgttc gtgcctcta tgtctctat ccggataca tgccgcctc tgtgtgacc ctgtcgatg gccaccttc ttctagacg gaggctaca ctaacaatc ttccaggta aaaaaccaa aaaacacta actcagtcg tttgcatgc cttctgtca ccggtaagt acactggct cggttcggt tacacacac aaaaccaag gtcatttgt ggagatatg tgagggcca gtatgtcaa ttgttggca tttttccgc cactttgtg atcctttag atgggcatt tgttagctg atgggtgtt acgtcaaca atgtaccag ctgacgtca tgcggagtt tgggggcca atcacataa tcgcgattg atgtgggta gtgtggatg accaagatt tgaccaatt acggggatg atttgtcgg ggtggtggt tgttgtgga aacggtgga atcccttca ctgatcccg tcaaagtac ccaatttac ccgatattc aatcaaggc tggcttatg tcagctgtg taagacagt tggaagaag taaaaaaca gtgattatt gtgaatacg tccgtccac ctatcgata agtataaaa cgctacaat tcggaagtt ttgatgaaa ttcgtgaag tcggttacc aacatggca aagcttact ttgaggaaa agtcaaaat cgggtaatt tacccatct tcaaagcgc caactgtta ccaataaac ccgaaacgg aacatattt tgtccgatt atactttca ccgatttag cacaaatgg tttgcaaag tctcaagac cttatgaag agtcatcaa gttcgagtt cttcaggcg atgagtttg aagatggcc aggacggtt acgcatctg aacctacag tgtgtctaa tggataata aaattgata aactcctac atatgagaa gagccggcg gttctcttt cattatccg aaaatgaaa tagaatccg aagttgagt tcgatgtaa caaatagga aaagtgaaa aagaattga aattctcta gtctttagt actttagta gttaagttt gtttccctt tgttatcag ttaa
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