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Gene information
General information
TC000549 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 9922556..9924446
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000549
    iBeetle screen iB_02654

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 11.11% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 11.11% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000549 RA
      MGALKQTEE EKTLLPQDI GTDYTFKRK IVWFNAIGF 
      FILHLLALY GGYRLLHCH ILTPLFALA LMFVSGEGI 
      TLGAHRLYS HKAFKASFV VRLAVIILH TIAGQNCLY 
      IWVRDHRQH HKYSDTDAD PHNSNRGFF FSHIGWLMS 
      RKHPAVIAK GKTIDMSDL ENDSLVMLQ KEHYKFLYI 
      IFAIGIPIA IPIYGWNES FTNSLFISY FGRYILQLH 
      ATWLINSAT HLYGTKPYD KFMNPVENY FISMIALGE 
      GWHNYHHAF PSDYRAAEY GVRYSITTF LIDALAFFG 
      LVYDLKEAN SEQVKIRAV KKGDGSHPV FGKQKEMEV 
      NFSDRQVTA NG
    CDS sequence
    • >TC000549 RA
      atgggagcg ctcaaacaa accgaggag gaaaaaact 
      ctactacca caagacatc ggaaccgat tacacattc 
      aagcggaaa atcgtctgg ttcaatgct attgggttc 
      ttcatcttg cacctcttg gccctttat ggcggctac 
      cggctgctg cattgccac atcctgacg ccgcttttc 
      gccctagca ctgatgttc gtctctggc gaaggcatc 
      accctcggg gcccaccgc ctgtactcc cacaaggcc 
      ttcaaagcg tctttcgtc gtgcgatta gctgtgata 
      attttgcac accatcgcc ggtcagaat tgtctctac 
      atctgggtt cgcgaccac cgccagcat cacaaatac 
      agcgacacc gacgccgac ccccacaac tccaacagg 
      ggcttcttc ttctcgcac atcggctgg ctgatgagc 
      aggaagcac cccgctgtc atcgccaag ggcaagacc 
      atcgacatg agcgacctg gagaacgac tcgctcgtg 
      atgttgcag aaagaacat tacaagttt ctgtacatc 
      attttcgcc atcgggatc ccgatcgca atcccgatt 
      tacggctgg aacgagtct ttcaccaac tcgctcttt 
      atcagctat tttggaaga tacattctt cagttacac 
      gccacttgg ctgatcaac agcgctacg cacttgtac 
      gggaccaag ccctacgac aagttcatg aatccggtg 
      gagaattat tttatttcg atgattgct cttggcgaa 
      ggctggcat aactaccac cacgccttt ccttcggat 
      tatcgggcg gcggagtat ggcgttaga tactcgata 
      acgactttt ctgatagac gctcttgcc ttttttggc 
      ctggtttat gacttgaaa gaggccaac tcggagcaa 
      gtcaaaatc cgggcggtc aaaaagggg gatggcagc 
      cacccagtt ttcgggaaa cagaaggag atggaagtt 
      aactttagc gataggcag gttacggca aatggttaa
    mRNA sequence
    • >TC000549 RA
      gaataaaaa tcttctccg gttcatcca agtgcgatt 
      gtcggttca gatttccgc cactaagca ttaaaaatg 
      aagtgtcac cctttggga gttgtatca agtgactaa 
      tctgcgcat tcagtgaac acaatgaca attttaatg 
      cgttttttg atcaaatta gaattgtga ggtcccaca 
      gacgattgt cctaaaatt gcttcgacc cacagccta 
      taaaaaaag tattgcgat tattaaaaa actatcagt 
      cagagactt agtttcgac catgttgga tcaatttct 
      tcactgatc atgggagcg ctcaaacaa accgaggag 
      gaaaaaact ctactacca caagacatc ggaaccgat 
      tacacattc aagcggaaa atcgtctgg ttcaatgct 
      attgggttc ttcatcttg cacctcttg gccctttat 
      ggcggctac cggctgctg cattgccac atcctgacg 
      ccgcttttc gccctagca ctgatgttc gtctctggc 
      gaaggcatc accctcggg gcccaccgc ctgtactcc 
      cacaaggcc ttcaaagcg tctttcgtc gtgcgatta 
      gctgtgata attttgcac accatcgcc ggtcagaat 
      tgtctctac atctgggtt cgcgaccac cgccagcat 
      cacaaatac agcgacacc gacgccgac ccccacaac 
      tccaacagg ggcttcttc ttctcgcac atcggctgg 
      ctgatgagc aggaagcac cccgctgtc atcgccaag 
      ggcaagacc atcgacatg agcgacctg gagaacgac 
      tcgctcgtg atgttgcag aaagaacat tacaagttt 
      ctgtacatc attttcgcc atcgggatc ccgatcgca 
      atcccgatt tacggctgg aacgagtct ttcaccaac 
      tcgctcttt atcagctat tttggaaga tacattctt 
      cagttacac gccacttgg ctgatcaac agcgctacg 
      cacttgtac gggaccaag ccctacgac aagttcatg 
      aatccggtg gagaattat tttatttcg atgattgct 
      cttggcgaa ggctggcat aactaccac cacgccttt 
      ccttcggat tatcgggcg gcggagtat ggcgttaga 
      tactcgata acgactttt ctgatagac gctcttgcc 
      ttttttggc ctggtttat gacttgaaa gaggccaac 
      tcggagcaa gtcaaaatc cgggcggtc aaaaagggg 
      gatggcagc cacccagtt ttcgggaaa cagaaggag 
      atggaagtt aactttagc gataggcag gttacggca 
      aatggttaa gcagtagct agtcgtgta gtttgtacc 
      aaatgttaa gactattta gtgtatttg tattgttaa 
      aaccaaagc taagcccaa ataaatcgg atttgaggt 
      t
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