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Gene information
General information
TC000998 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 4025542..4034457
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000998
    iBeetle screen iB_02574
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Fertility
    ovary shape irregular - Number of animals showing the phenotype: 4
    tropharium potentially degenerated - Number of animals showing the phenotype: 4

    egg chamber mostly not present - Number of animals showing the phenotype: 4

    egg chamber potentially degenerated - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection



    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection



    Pupal morphology
    legs potentially shortened - Number of animals showing the phenotype: 8
    iBeetle screen iB_02653
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Fertility
    egg chamber not present - Number of animals showing the phenotype: 4

    tropharium structure irregular - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000998 RA
      MPKSMNVRV TTMDAELEF AIQQTTSGK QLFDQVVKT 
      IGLREVWFF GLQYTDSKG DLTWIKLYK KVMSQDVKK 
      ENPLQFKFR AKFYPEDVA EELIQDITL RLFYLQVKN 
      SILSDEIYC PPETSVLLA SYAVQARYG DYCKAVHTS 
      GFLSNDRLL PQRVMDQHK MSKEEWEQS IMTWWKEHR 
      GMLREDTMM EYLKIAQDL EMYGVNYFE IRNKKGTEL 
      YLGVDALGL NIYEKDDRL TPKIGFPWS EIRNISFND 
      RKFIIKPID KKAPDFVFF APRVRINKR ILSLCMGNH 
      ELYMRRRKP DTIDVQQMK AQAREEKLA KQQQREKLQ 
      LEIAARERA EKKQQEYED RIKAMQEEM ERSQANLLE 
      AQEMIRRLE EQLKQLQAA KEELEKRQN ELQAMMERL 
      EESKNMEAA ERQKLEEEI QAKQLEVQR IQDEVTAKD 
      NETKRLQEE VENARRKEE ELKAQQMAN ATKGHLEEN 
      NHDEDDEMV NGDVSKDLT TDENIVDPV EDRRTLAER 
      NERLHDQLL ALKKDLAQS RDETKETAM DKIHRENVR 
      QGRDKYKTL REIRKGNTK RRVDQFENM
    CDS sequence
    • >TC000998 RA
      atgccgaaa tcgatgaat gtcagggtg accactatg 
      gatgcggaa ctcgagttc gcaatccag caaacaaca 
      tccggaaaa caactcttc gatcaagta gttaaaaca 
      atcggattg agagaagtt tggtttttc ggactacag 
      tacaccgat tctaagggt gatctaaca tggatcaag 
      ctgtacaaa aaggtgatg agccaagat gtgaagaaa 
      gaaaatcca cttcaattc aagttccgt gccaaattt 
      tatcccgaa gatgtcgcc gaagaatta attcaagac 
      atcactttg cgtttattt tatttacaa gttaaaaac 
      tctatctta tctgatgag atttattgt cctcctgaa 
      acgtccgtc cttttagct tcctatgcc gttcaagcg 
      agatacggc gattattgt aaggccgtt catacctcc 
      ggattcctt tcaaacgac agactttta ccacaaaga 
      gtcatggac cagcacaag atgtcgaaa gaggagtgg 
      gagcagagc attatgacc tggtggaag gagcatcgt 
      ggtatgttg cgcgaagac accatgatg gaatacctg 
      aaaatagcc caggacttg gaaatgtac ggtgtgaac 
      tactttgaa attcgcaac aaaaaaggc accgaactg 
      tatttgggc gtggacgcc ttgggtctg aacatttac 
      gaaaaagac gaccgacta acgcccaaa atcggcttt 
      ccctggtcg gaaatccgc aatatttcg ttcaacgac 
      cgcaaattc attataaag ccaatcgat aagaaggcc 
      cccgatttc gtatttttc gcgcctcgc gttcgaata 
      aacaaacga attttatct ctatgcatg ggtaatcac 
      gagttgtac atgcgacgt cggaagccc gacaccata 
      gacgtgcag cagatgaaa gcccaagct cgtgaagaa 
      aaactagcc aaacaacaa caaagggaa aaactccaa 
      ttggagatt gcggcgaga gaacgcgcc gagaagaaa 
      caacaagag tatgaagat aggattaaa gcaatgcaa 
      gaggagatg gagcgaagc caagccaat ctcctcgag 
      gcacaagag atgatccgg cgcctggag gagcaactg 
      aagcagttg caagcggcc aaggaggag ctggagaag 
      aggcagaac gagctgcag gcgatgatg gagcgtttg 
      gaggaaagc aagaacatg gaagctgcc gaacgccaa 
      aagttggag gaggagatt caagccaaa cagttggag 
      gtgcagagg atccaagac gaggtgact gcgaaggac 
      aatgaaact aagcgtctg caagaggag gtcgagaac 
      gcgcgtcgc aaggaagag gagctgaag gcccagcaa 
      atggccaat gcgaccaaa ggccacctg gaggagaac 
      aaccacgat gaggacgat gagatggtc aatggggac 
      gtgagcaag gatttgacc acggatgag aacattgtg 
      gatcctgtg gaagacaga cgcactctt gctgagcgc 
      aatgagcga ctccatgat caacttctg gctttgaag 
      aaagacctc gcccagtcg cgggacgag acgaaggaa 
      accgccatg gacaagatc catcgagag aacgtgcgc 
      cagggcaga gataaatac aaaactctg cgtgagata 
      cgtaaaggt aacactaag cgtcgcgtg gaccaattc 
      gaaaatatg taa
    mRNA sequence
    • >TC000998 RA
      tttggggta gaacccttg gttttgtgg tcgtttggt 
      agatggcag tatatcgcg catttttgg tcctctggt 
      ggcaaagtc ggaactaaa cgatacaca atttgctga 
      ttcttgaca atttatttt ccgataagt tgtcgtgtc 
      gtgtgtgga agggtgtgt gcccagaaa aaccccgcg 
      tgtgctact taatgtggt ggctacaag agtgttaat 
      cttcaaaag atatatttc ttagttttt tggttcgag 
      acaagactg ccaacatgc cgaaatcga tgaatgtca 
      gggtgacca ctatggatg cggaactcg agttcgcaa 
      tccagcaaa caacatccg gaaaacaac tcttcgatc 
      aagtagtta aaacaatcg gattgagag aagtttggt 
      ttttcggac tacagtaca ccgattcta agggtgatc 
      taacatgga tcaagctgt acaaaaagg tgatgagcc 
      aagatgtga agaaagaaa atccacttc aattcaagt 
      tccgtgcca aattttatc ccgaagatg tcgccgaag 
      aattaattc aagacatca ctttgcgtt tattttatt 
      tacaagtta aaaactcta tcttatctg atgagattt 
      attgtcctc ctgaaacgt ccgtccttt tagcttcct 
      atgccgttc aagcgagat acggcgatt attgtaagg 
      ccgttcata cctccggat tcctttcaa acgacagac 
      ttttaccac aaagagtca tggaccagc acaagatgt 
      cgaaagagg agtgggagc agagcatta tgacctggt 
      ggaaggagc atcgtggta tgttgcgcg aagacacca 
      tgatggaat acctgaaaa tagcccagg acttggaaa 
      tgtacggtg tgaactact ttgaaattc gcaacaaaa 
      aaggcaccg aactgtatt tgggcgtgg acgccttgg 
      gtctgaaca tttacgaaa aagacgacc gactaacgc 
      ccaaaatcg gctttccct ggtcggaaa tccgcaata 
      tttcgttca acgaccgca aattcatta taaagccaa 
      tcgataaga aggcccccg atttcgtat ttttcgcgc 
      ctcgcgttc gaataaaca aacgaattt tatctctat 
      gcatgggta atcacgagt tgtacatgc gacgtcgga 
      agcccgaca ccatagacg tgcagcaga tgaaagccc 
      aagctcgtg aagaaaaac tagccaaac aacaacaaa 
      gggaaaaac tccaattgg agattgcgg cgagagaac 
      gcgccgaga agaaacaac aagagtatg aagatagga 
      ttaaagcaa tgcaagagg agatggagc gaagccaag 
      ccaatctcc tcgaggcac aagagatga tccggcgcc 
      tggaggagc aactgaagc agttgcaag cggccaagg 
      aggagctgg agaagaggc agaacgagc tgcaggcga 
      tgatggagc gtttggagg aaagcaaga acatggaag 
      ctgccgaac gccaaaagt tggaggagg agattcaag 
      ccaaacagt tggaggtgc agaggatcc aagacgagg 
      tgactgcga aggacaatg aaactaagc gtctgcaag 
      aggaggtcg agaacgcgc gtcgcaagg aagaggagc 
      tgaaggccc agcaaatgg ccaatgcga ccaaaggcc 
      acctggagg agaacaacc acgatgagg acgatgaga 
      tggtcaatg gggacgtga gcaaggatt tgaccacgg 
      atgagaaca ttgtggatc ctgtggaag acagacgca 
      ctcttgctg agcgcaatg agcgactcc atgatcaac 
      ttctggctt tgaagaaag acctcgccc agtcgcggg 
      acgagacga aggaaaccg ccatggaca agatccatc 
      gagagaacg tgcgccagg gcagagata aatacaaaa 
      ctctgcgtg agatacgta aaggtaaca ctaagcgtc 
      gcgtggacc aattcgaaa atatgtaaa tcacaatca 
      aaatcaaaa ttccatatt caaactttc aatgctcaa 
      tacgagatc atgttaaat ttaagtttt ggtttactt 
      acgtctaag tatgatttt tttccaatg taagataaa 
      aagtgctca tttaagtgg ccttagact ttcaaatct 
      tagttacac tattttcat tcttgtttt gttgcaaat 
      tattttgtt agaattgtg atatgatat gagaggaga 
      aaaaaacag atctgttaa ttcactttc tttacattt 
      aaatcactt aaacgcatt ccacgtaat ggtagtttt 
      tcttctaat tttagtgcc atttggtta acgggctac 
      acaaaaata atctttgtt ttttgaaat tcgaattga 
      ttctaagat cagctaaat tttgtacac ttttattgg 
      cctggtgct tccttattg tgtaaataa aagttatac 
      aattcgttt tttctactt actgtattt ttatcgtgg 
      ttaaaaaga aggttttta caatatttt ttctacaat 
      acttaatct tttacctgt tacatatcg taacaataa 
      agtaatgta cataacatt tatttttta aagaccatg 
      atgccttct agataggtt tggatgata tttacactg 
      tagtatgtc agatgggtg aggaaaaac cgttcttca 
      aatggtttt ttaggaaag aaattaact aatccttat 
      ttaaataaa ccgcagata tcaaacgtc tgttttatt 
      atacattta tataatata tatttatct tgcgatcac 
      ttgtatatt gtacatcct aaaactacg tcgctagtt 
      tttaacatt tgtattctt cgtagttta tgagttatg 
      tactactag tataattaa taaatatta tttggaaaa