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Gene information
General information
TC000603 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 3631852..3633566
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000603
    iBeetle screen iB_02652
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000603 RA
      MEDESPTPK KHTDKNKHP KEDTILNHL EISVAESEK 
      RANGTLNLR EFYTVYLIE TKLTDSEFQ NKFPKLTTV 
      WRRYTEFEQ LRGYLEVTY PYIILPPLP EKRVMFGWQ 
      KISSDTFDP NFVDRRRAG LENFLLRVA SHPVLSWDK 
      HFIEFLQHE DDWRETYKS NGYLQLMEN KLKALNLSM 
      RLRNTDPRF ESIKDYSHV FQNNLNNLL KARSRVAEK 
      QYNVHKLHA NYGRVFSEW SAVEKDMGD ALQKTGHYL 
      DSLASSIDA GLEDEELLV DQLKEYLFF ANSLQNVCK 
      NYEYHQLQL ESAEDNVAS KNVERNKAQ QGKTSLMSR 
      LFGAVDTDE VRELKVNLL DQQIQEGAA AVNVSKTNL 
      NDFSTKALD DIERFQKQK VVDLQETLT SYAFLQLKT 
      AKKGLQTWL QIRDCLQNV P
    CDS sequence
    • >TC000603 RA
      atggaggat gaaagcccc acacccaaa aaacacacc 
      gacaaaaat aagcacccc aaagaggac acgattctc 
      aatcacctg gaaatttcg gtggcagag tctgaaaaa 
      cgcgccaac ggaacgctt aatctacgc gaattttac 
      acagtttat ttaattgag accaaatta acagacagt 
      gaattccaa aacaaattt cctaaatta acaaccgta 
      tggcggcgt tatacagaa ttcgagcaa ctgcggggc 
      tatctggag gtcacctac ccgtacata attttgccc 
      cctttaccc gagaagaga gtcatgttt gggtggcaa 
      aaaatctca agtgacact ttcgacccg aatttcgtc 
      gaccggcga agggccggc ctcgaaaat tttctttta 
      agagtcgcc tcacacccg gttctgagc tgggataaa 
      cactttatt gagtttttg cagcatgag gatgactgg 
      agggagact tacaagagc aacggttac ttgcagctg 
      atggaaaac aaactcaaa gccttaaac ctctcaatg 
      aggctccga aacaccgac cctagattc gaatcaatc 
      aaagactac agtcacgtt ttccaaaac aacttaaac 
      aacttgctt aaggcgcgt tcccgcgtc gctgaaaaa 
      caatacaac gtgcacaag ctccacgcc aactacggc 
      cgagttttc agcgaatgg agcgccgtt gaaaaggac 
      atgggcgat gccttgcaa aaaacaggc cattatctc 
      gattctttg gcttcaagc attgatgcg gggcttgaa 
      gacgaggaa ctgctagtg gaccaattg aaagagtac 
      ttatttttc gcaaattcg ctacaaaac gtgtgcaaa 
      aactacgag taccaccaa ctacaactc gagagcgcg 
      gaagacaac gtcgctagc aaaaatgtg gagagaaat 
      aaagcccag cagggcaaa actagcctc atgtcgagg 
      ctttttggg gctgtggat acagacgaa gtcagggaa 
      ctcaaagtc aatttgttg gaccagcag atacaggaa 
      ggggcagca gctgtcaac gtctcaaaa actaattta 
      aatgatttt tccacaaag gccttagat gatattgaa 
      cgatttcag aaacaaaaa gtcgttgat ttgcaagaa 
      acgttaacg agttacgcg ttcttgcaa ttaaaaact 
      gccaaaaag ggcctccag acctggctt cagatacgt 
      gattgtttg caaaatgta ccttaa
    mRNA sequence
    • >TC000603 RA
      aaacatgtg acttcgttt cacatcaaa acaaggaca 
      taattgtgt ttttttaca tgacactga cagttaacc 
      tagatgctg gctgacctg tcaaatttg acaaccgta 
      ccgtagaaa aaactcatt tcttttaaa tttctctgc 
      ggaagtgaa atggaggat gaaagcccc acacccaaa 
      aaacacacc gacaaaaat aagcacccc aaagaggac 
      acgattctc aatcacctg gaaatttcg gtggcagag 
      tctgaaaaa cgcgccaac ggaacgctt aatctacgc 
      gaattttac acagtttat ttaattgag accaaatta 
      acagacagt gaattccaa aacaaattt cctaaatta 
      acaaccgta tggcggcgt tatacagaa ttcgagcaa 
      ctgcggggc tatctggag gtcacctac ccgtacata 
      attttgccc cctttaccc gagaagaga gtcatgttt 
      gggtggcaa aaaatctca agtgacact ttcgacccg 
      aatttcgtc gaccggcga agggccggc ctcgaaaat 
      tttctttta agagtcgcc tcacacccg gttctgagc 
      tgggataaa cactttatt gagtttttg cagcatgag 
      gatgactgg agggagact tacaagagc aacggttac 
      ttgcagctg atggaaaac aaactcaaa gccttaaac 
      ctctcaatg aggctccga aacaccgac cctagattc 
      gaatcaatc aaagactac agtcacgtt ttccaaaac 
      aacttaaac aacttgctt aaggcgcgt tcccgcgtc 
      gctgaaaaa caatacaac gtgcacaag ctccacgcc 
      aactacggc cgagttttc agcgaatgg agcgccgtt 
      gaaaaggac atgggcgat gccttgcaa aaaacaggc 
      cattatctc gattctttg gcttcaagc attgatgcg 
      gggcttgaa gacgaggaa ctgctagtg gaccaattg 
      aaagagtac ttatttttc gcaaattcg ctacaaaac 
      gtgtgcaaa aactacgag taccaccaa ctacaactc 
      gagagcgcg gaagacaac gtcgctagc aaaaatgtg 
      gagagaaat aaagcccag cagggcaaa actagcctc 
      atgtcgagg ctttttggg gctgtggat acagacgaa 
      gtcagggaa ctcaaagtc aatttgttg gaccagcag 
      atacaggaa ggggcagca gctgtcaac gtctcaaaa 
      actaattta aatgatttt tccacaaag gccttagat 
      gatattgaa cgatttcag aaacaaaaa gtcgttgat 
      ttgcaagaa acgttaacg agttacgcg ttcttgcaa 
      ttaaaaact gccaaaaag ggcctccag acctggctt 
      cagatacgt gattgtttg caaaatgta ccttaattt 
      tttgtttgt tatttttta tttatgtgc aatctattt 
      atgtactct aaaaacagt atttatatt tctattgta 
      gctttgtta aaactgagg ctgtacgaa atactctta 
      aataaatta tttttgttt ta