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Gene information
General information
TC000916 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 2870456..2909200
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000916
    iBeetle screen iB_02650
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000916 RA
      MSIFNPGPV CRPDPNSFP IISPIIDLE VVRRSSTAP 
      RRTCLDKDN LVAIPFLTK LEVQCKMNL SAQFSDYWT 
      NSFYQNYKT NPFLASDCD KNQNTEQNY THNNEMYHR 
      VKEEPIYES CRFSINQPY LNHFDNSVT PMVNHDFTQ 
      HLSQYDTCQ QGIYPENKT LINETSLQL DENKLDKKN 
      DDSPALRAL LTRPQAKKT PPNQYENFQ QYDNNNFSA 
      EENSVSEPP ANFYPWMKA HGDSSATGN KRTRQTYTR 
      YQTLELEKE FHFNKYLTR RRRIEIAES LRLTERQIK 
      IWFQNRRMK AKKDTKFTE QSVTSTFDF LSLHPAETS 
      MNDANRNDC AKPLTQIRN IPGPPETP
    CDS sequence
    • >TC000916 RA
      atgagcatt tttaaccca ggaccagtt tgccgccct 
      gaccccaac tctttccca attatttcg ccgataatt 
      gatctggaa gtggttagg cgctcgtct acagctcca 
      cgccgaacg tgcctggac aaggacaac ttagtcgca 
      attccattt ttgaccaag ttggaagtg cagtgcaag 
      atgaatcta tcggctcag tttagtgac tattggaca 
      aactccttc taccaaaac tacaaaacc aacccgttc 
      ttggccagt gactgtgac aaaaaccaa aacaccgaa 
      cagaactat acccacaat aatgaaatg taccaccgt 
      gtcaaggaa gaaccgatc tacgaaagc tgcaggttt 
      tccattaat caaccatat ttaaaccat tttgataat 
      tccgtcacg ccgatggta aatcacgac ttcacccaa 
      catctgagt cagtacgac acctgtcag caaggtatt 
      tatcccgaa aataaaacc ctaattaat gaaacaagc 
      ctccaactc gatgaaaat aaattagac aagaaaaac 
      gatgattca cccgcttta cgtgccctg ttaacgcga 
      ccacaagcc aagaaaact ccccccaat caatacgaa 
      aacttccag caatacgac aacaacaat ttcagtgct 
      gaggaaaat agtgttagt gaacccccc gcaaatttc 
      tacccttgg atgaaagct cacggtgac tcctcagcc 
      actggaaac aaacggacg cggcagacc tacacccgt 
      taccaaacc ctcgagctc gagaaagag tttcatttc 
      aacaaatat ctgacaaga cgccgccga atcgaaatc 
      gccgaatct ttgcgtctg actgagcgc caaatcaaa 
      atttggttc caaaatcgg cgaatgaaa gcgaaaaag 
      gacacaaag ttcaccgag cagagtgta acgtccact 
      tttgacttt ctctctctt cacccagcg gagacttca 
      atgaacgat gcgaacagg aacgactgt gccaaaccc 
      ttgacccaa attaggaat attccgggg cccccggaa 
      acgccttag
    mRNA sequence
    • >TC000916 RA
      ctgctgaag ccggggatt cttcaagga aaacattgg 
      cctggaagt tggagttat tgttgaccg tgctttcca 
      acgaaggga gagaaaagg gagaattaa cggaaggag 
      cactcgccg cgtaaacac atccagaaa aagacgcaa 
      ttagggacg agctttcga ttaaaatga gcattttta 
      acccaggac cagtttgcc gccctgacc ccaactctt 
      tcccaatta tttcgccga taattgatc tggaagtgg 
      ttaggcgct cgtctacag ctccacgcc gaacgtgcc 
      tggacaagg acaacttag tcgcaattc catttttga 
      ccaagttgg aagtgcagt gcaagatga atctatcgg 
      ctcagttta gtgactatt ggacaaact ccttctacc 
      aaaactaca aaaccaacc cgttcttgg ccagtgact 
      gtgacaaaa accaaaaca ccgaacaga actataccc 
      acaataatg aaatgtacc accgtgtca aggaagaac 
      cgatctacg aaagctgca ggttttcca ttaatcaac 
      catatttaa accattttg ataattccg tcacgccga 
      tggtaaatc acgacttca cccaacatc tgagtcagt 
      acgacacct gtcagcaag gtatttatc ccgaaaata 
      aaaccctaa ttaatgaaa caagcctcc aactcgatg 
      aaaataaat tagacaaga aaaacgatg attcacccg 
      ctttacgtg ccctgttaa cgcgaccac aagccaaga 
      aaactcccc ccaatcaat acgaaaact tccagcaat 
      acgacaaca acaatttca gtgctgagg aaaatagtg 
      ttagtgaac cccccgcaa atttctacc cttggatga 
      aagctcacg gtgactcct cagccactg gaaacaaac 
      ggacgcggc agacctaca cccgttacc aaaccctcg 
      agctcgaga aagagtttc atttcaaca aatatctga 
      caagacgcc gccgaatcg aaatcgccg aatctttgc 
      gtctgactg agcgccaaa tcaaaattt ggttccaaa 
      atcggcgaa tgaaagcga aaaaggaca caaagttca 
      ccgagcaga gtgtaacgt ccacttttg actttctct 
      ctcttcacc cagcggaga cttcaatga acgatgcga 
      acaggaacg actgtgcca aacccttga cccaaatta 
      ggaatattc cggggcccc cggaaacgc cttagattc 
      gcctacgtg acacgaacc ggagcaaat atcaaaaaa 
      ttgattgta aatacgtgg attagttta ttttgcgtg 
      aaagtcact aattgtagt taatttgtg tatagagag 
      tttgaataa aaggataag tcgtcg