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Gene information
General information
TC030989 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 6602837..6614057
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC030989
    iBeetle screen iB_02649

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030989 RA
      MALSTVLLV VLVLVREPR GFFDAVDAL GARYEHDAL 
ASARCRASC WHQTNKNSC VKSCLAAPF NKPGRCPQN 
EKTLSPFDA VCLKTCTQD NQCSNLKKC CRHSCGVTC 
QHPEELLSA VGIPPIPQD LKLTEGLKK RTVHIEWNP 
GQPTPGVVL YLLEERHHT GRNFFGEDL SDWSQCGQT 
NKTSHILRN FVKPARWYQ FRVAAVNEN GTKGFSNIS 
PTFSVSARP RPPRAPQNV TVSPLSSDS NGTLSGELH 
WAPPLSELP IQKYKVFWS RRLHGDKAL NSVLVNQRV 
VSKEETTFL LQNLQPNSL YFLQIQALA QYGNSRLKG 
EKSGLVLNT TNYMNVTHN LVSTKPPIR NKIQDLQVL 
KIYWAQNSL KAKIIWKPG KDSSKYAVS LWAWPCHKN 
SKSRNHFKL AVTTKMSHY VLTDLEFEC RYRVSVKSI 
SEEGIKSAE DTSITFLIP SCSEFKKTN RKAKCTN
    CDS sequence
    • >TC030989 RA
      atggctctg tccacggta ctcctagta gtactagtg 
ctagtacgc gaaccgcgg ggttttttc gacgccgtc 
gacgctctc ggggcgcgc tacgagcac gacgccctc 
gcctccgcg cgctgcaga gccagctgc tggcaccag 
accaacaaa aactcgtgc gtaaaatcg tgcctagct 
gcacctttt aataaaccc ggtcgttgt ccacaaaac 
gagaaaacc ttatcacct ttcgacgcc gtgtgcttg 
aagacatgc actcaggat aatcagtgt tcaaatttg 
aaaaaatgt tgccgacac agttgcgga gtcacttgc 
caacatccc gaagaactg ttatcagca gtgggcatt 
ccccccatc ccccaagac ctaaaactg accgaaggt 
ttaaaaaaa cgcacggtc cacatcgaa tggaaccct 
ggacaacca actcctgga gtcgttctc tatttgctg 
gaagagcgt caccacacc ggacgcaac ttcttcggc 
gaagaccta agcgactgg tcgcagtgc ggccaaact 
aacaaaact agtcacatt ttacgaaat tttgttaaa 
cctgcccga tggtaccaa ttccgggtg gccgcagtc 
aacgaaaat ggtaccaaa ggattttcc aatatttct 
cccactttt agcgtttcc gcaagacct aggcccccc 
agagccccc caaaacgtg actgttagc cccctttcg 
agcgactca aacgggact ttgagtggg gaattgcat 
tgggcgccc ccactttcc gaattgccg attcagaaa 
tataaagtg ttttggagt cggagattg catggggat 
aaagcgctc aattctgtt cttgttaat caaagagtg 
gtgtcaaag gaggaaaca acgttttta ttgcaaaac 
ctccaacca aactctttg tatttcctt caaattcag 
gcattagct caatatggt aatagccgc ctaaaggga 
gaaaaatcg ggacttgtt ctaaacaca acaaactac 
atgaatgtt actcataat ttagtctcg actaaaccg 
ccgattaga aacaaaatc caagactta caggtgcta 
aaaatctac tgggctcag aatagttta aaagcgaaa 
atcatctgg aaacctggg aaggactct tcgaaatat 
gccgtgtct ttatgggct tggccttgc cacaagaat 
tccaaatct cgaaaccat ttcaaatta gcagtgacg 
acaaaaatg tctcattac gttttaact gatctcgaa 
tttgagtgt cgatatagg gttagtgtt aaaagtatt 
tcagaagaa ggaataaag tcagcggag gacacttcg 
ataacgttt cttattccc tcctgtagc gaatttaaa 
aaaacgaat cgaaaggcg aaatgtaca aattaa
    mRNA sequence
    • >TC030989 RA
      tcaaagcga tggctctgt ccacggtac tcctagtag 
tactagtgc tagtacgcg aaccgcggg gttttttcg 
acgccgtcg acgctctcg gggcgcgct acgagcacg 
acgccctcg cctccgcgc gctgcagag ccagctgct 
ggcaccaga ccaacaaaa actcgtgcg taaaatcgt 
gcctagctg cacctttta ataaacccg gtcgttgtc 
cacaaaacg agaaaacct tatcacctt tcgacgccg 
tgtgcttga agacatgca ctcaggata atcagtgtt 
caaatttga aaaaatgtt gccgacaca gttgcggag 
tcacttgcc aacatcccg aagaactgt tatcagcag 
tgggcattc cccccatcc cccaagacc taaaactga 
ccgaaggtt taaaaaaac gcacggtcc acatcgaat 
ggaaccctg gacaaccaa ctcctggag tcgttctct 
atttgctgg aagagcgtc accacaccg gacgcaact 
tcttcggcg aagacctaa gcgactggt cgcagtgcg 
gccaaacta acaaaacta gtcacattt tacgaaatt 
ttgttaaac ctgcccgat ggtaccaat tccgggtgg 
ccgcagtca acgaaaatg gtaccaaag gattttcca 
atatttctc ccactttta gcgtttccg caagaccta 
ggcccccca gagcccccc aaaacgtga ctgttagcc 
ccctttcga gcgactcaa acgggactt tgagtgggg 
aattgcatt gggcgcccc cactttccg aattgccga 
ttcagaaat ataaagtgt tttggagtc ggagattgc 
atggggata aagcgctca attctgttc ttgttaatc 
aaagagtgg tgtcaaagg aggaaacaa cgtttttat 
tgcaaaacc tccaaccaa actctttgt atttccttc 
aaattcagg cattagctc aatatggta atagccgcc 
taaagggag aaaaatcgg gacttgttc taaacacaa 
caaactaca tgaatgtta ctcataatt tagtctcga 
ctaaaccgc cgattagaa acaaaatcc aagacttac 
aggtgctaa aaatctact gggctcaga atagtttaa 
aagcgaaaa tcatctgga aacctggga aggactctt 
cgaaatatg ccgtgtctt tatgggctt ggccttgcc 
acaagaatt ccaaatctc gaaaccatt tcaaattag 
cagtgacga caaaaatgt ctcattacg ttttaactg 
atctcgaat ttgagtgtc gatataggg ttagtgtta 
aaagtattt cagaagaag gaataaagt cagcggagg 
acacttcga taacgtttc ttattccct cctgtagcg 
aatttaaaa aaacgaatc gaaaggcga aatgtacaa 
attaaactg taacatata tttataact ttgtttcgt 
gaccgaata aatgtttta caaaaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY