Status
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Gene information
General information
TC032067
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC000833,TC000834
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 6462766..6475802
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032067
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02648

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032067 RA
      MPKVQPKVA KKIITGHAT EYSNKPQIV AADLPISDR 
      ATKRVIAPF VRIVRKGQK PHEAEESKH FSFFLQRKP 
      SVLIRDLSS FLSDSVDLP SLLHETADV LKSITNAAG 
      VTLYMMDAA TNEIYQSNK FPQRDRYKI RWKIQEGTT 
      TAVYVAYKK NYVLIDDII EDDRFPEGI GYETETIKS 
      VLCVPVVTP DGECFAVIE LYRQLFDEP FSKDDLKIT 
      LIVTGWMGA AIHQNSRRL ALQRQQELN DYLLDLTKC 
      FFGESVVME KMISEIVNF AKVTLNAEK GTIFIIDRD 
      SGDDLVADM YDDTEDGKE FKKAQKVRF SKERGIASL 
      VARSGITVN VKDAYKDAR FNKEIDQKT GFITRSILC 
      MPIVGVEGI LGVVQVVNK KRGGHFTST DESLFRTFC 
      VYCALALRF TKVQKRMQS TRLLNNCFL KMIQLQVKP 
      CIHDIITYN VANSQVVIP MDFDHFGWH INTEDHPKM 
      PQYILYMFL QLIKNTLHP ERCIHYILS VKKFYRRNP 
      YHNFEHAFN VVHCMYLIL KRNIKQFTE TEIKALMIA 
      ALCHDVDHP GFTNNFLEL SNNDLANLY ENSCLENHQ 
      YYVAMLLLD HNKVLTHLS KEEYEVLRT EIKQAIIAT 
      DPLVYFANQ INLEKICNT HSFDWGNET HRYYLKGTM 
      MMVADLSGM CKPFLVSKR ITDNLYAEC YLQGDLEKE 
      MGLCPLSMM DRDKSNFIP EDQVTFITT VILPCLDIL 
      AQLLPNTEA LYSDTKLLV QNWQDIIDV RGKKCWRQD 
      DSILSHLPK SPRTHFYRQ TFQAKDDKL LSFFLQKTP 
      SVLLRELSS FLTDSVDLP SLLHETCDV LKNVTKAFG 
      VTLYMVDPN NDEIYLTKK KASEWPKHR WTIEQGTIV 
      AAFVAYKKE YVMLEDVTR DERFPEGIA RKGEAVKSV 
      LCVPVVTPD GDCLAVIEL YRQSDQGPF SKDDLKITI 
      VVTGWMGAA IHQNQQRVA LKKQQELNE YLLDLTKNY 
      FSDNVVMEK MISDVVKFA KKTLGAERG SFFIIDKEN 
      EELVADLFD EGIDEQDTG LYKKNTKIR FGKERGIAG 
      LVARTGVAC NIKDAYNDS RFNKEIDQK TGFTTKSIL 
      CMPIMGVEG ILGVVQVVN KINGGYFTN TDEDLFKTF 
      SVYCALALH YTKVNQQMN KMMHVSNIS LKLLRLQIK 
      PCSHDIEEF IQHPDPKIS PDFYQFSWY INLNSFDQM 
      PQYCLYMIL QVVHVSEIN IKNMMQFIL SARKLYRNN 
      PYHNFEHAF NVCHCMHAI LRRNLKKFS VVELKALII 
      AALCHDLDH GGFTNNFLQ LINDDLSQL YDESFLENH 
      HFRVTMTIL NDCEIYSNL SETTYKIIS KEIKEAILA 
      TDLALYFKF RAKLAQICN DSLFDWGNP VHRNFVKAI 
      MMTSCDLSG QCKPFYVAK RITDNLYRE FYHQGDMEK 
      KMGLHPLSI MDREKQHTI PEDQVQFLS IVVIPCVDL 
      LRVVLPNTE DLYNEACTL RDTWQEMIH KRGTTTPTK
    CDS sequence
    • >TC032067 RA
      atgcctaaa gtccagccc aaagtggcg aaaaagata 
      atcaccggc catgccact gaatacagc aataagccc 
      cagatcgtg gctgccgat ctgccaatt agtgataga 
      gctacaaag agagtcatc gctccgttt gtccgaatc 
      gtccgaaag ggacaaaag ccgcacgaa gctgaagaa 
      agcaaacat tttagtttt tttcttcaa aggaaaccg 
      agtgttcta atccgcgac ctatcctcc tttttatcc 
      gatagtgtc gatctgcca tctttacta cacgaaact 
      gccgacgtc ttgaaaagc ataacgaac gcagcggga 
      gttacttta tacatgatg gacgctgca actaacgaa 
      atttaccaa agtaacaaa ttcccgcag cgggaccgc 
      tacaagatc aggtggaaa atccaagaa gggacaacc 
      acagcagtt tatgtggcg tacaaaaaa aactacgtt 
      ttgattgat gatattatt gaagacgac cgctttcct 
      gaagggatc ggatacgaa actgagact attaagtcg 
      gtgctttgc gtccctgtt gtaacaccc gatggggaa 
      tgtttcgca gttattgag ctctaccgt caattattt 
      gacgagccg ttctcaaaa gatgacctc aaaatcacc 
      ctcattgtc acagggtgg atgggggct gctatccac 
      caaaactcg cgtagactt gccctccaa cgccaacaa 
      gaactcaac gactatctt ctagatttg actaaatgc 
      ttctttggc gagtcggtt gtgatggaa aaaatgatt 
      tcagaaata gtcaatttc gcaaaagtg actcttaac 
      gctgagaaa ggaacaatt tttattatc gatcgggac 
      agtggagat gatcttgtt gccgatatg tacgatgat 
      accgaggac ggaaaagaa ttcaaaaaa gcacaaaaa 
      gtgcgtttt tcgaaagag cgcggaatt gcgagtctt 
      gtggcccga tcagggatc acagtcaat gttaaagat 
      gcgtataag gatgcacgt ttcaataag gaaattgat 
      cagaaaaca ggatttatc acaagatca attctgtgt 
      atgcctata gtcggagta gaaggaatt ttaggagta 
      gtacaagtt gtaaataag aaaagaggt ggtcatttc 
      acaagtact gacgaatcg ttgtttaga accttctgt 
      gtgtattgt gcacttgct ttgcgattc actaaagtg 
      caaaagcgg atgcagtca acgagactg ttgaataat 
      tgtttcctg aagatgatc caactgcaa gtgaaacct 
      tgcatccac gatatcatc acatacaac gtggcaaac 
      tcacaagtg gtgatcccc atggatttt gaccacttt 
      gggtggcac attaataca gaagatcac ccgaaaatg 
      ccccagtat atactttac atgttcctc caattgatt 
      aaaaataca ctacatcct gaacgttgc atccactac 
      atcctttcg gtgaaaaaa ttctacagg cggaatcct 
      taccacaac ttcgagcat gcgttcaat gtagtccat 
      tgcatgtat cttatattg aaacgaaac atcaaacaa 
      tttactgag actgaaatt aaagcctta atgattgct 
      gctctctgc cacgatgtg gaccatcca ggtttcacg 
      aataatttc ctcgagttg agtaacaac gatttggcc 
      aatttgtac gaaaattcg tgtttggaa aaccaccag 
      tattacgtg gctatgttg ttactcgac cataataaa 
      gttctgaca cacctttca aaagaggag tacgaggtt 
      ttgaggaca gaaatcaaa caagcaatc atcgcaact 
      gatcctttg gtttacttt gcgaaccaa atcaatttg 
      gagaagatt tgcaatacg cattcgttt gactggggt 
      aatgagaca caccgttat tatttgaaa gggaccatg 
      atgatggtt gctgatctc tcggggatg tgtaagccg 
      tttctggtg tcgaaacga attacggac aatttatat 
      gccgagtgt tacttgcaa ggggatttg gagaaggaa 
      atggggctt tgtccctta tctatgatg gatagggac 
      aagagcaat tttattcca gaggatcaa gttactttt 
      ataaccact gtgatactc ccttgtttg gacattttg 
      gcccaactt ttgccaaac actgaggcg ttatacagc 
      gacaccaaa ttattggtc caaaactgg caagatatt 
      atcgacgtc agaggcaag aaatgctgg cgccaggac 
      gactctatt ttatcacac ctccctaag tccccccga 
      acccacttt tatcgccaa acatttcaa gcaaaagac 
      gacaagctc ttgagcttc ttcctgcag aaaacccca 
      agtgtgctt ttacgcgag ctttcgtcc tttctgact 
      gacagtgtt gaccttccg tctcttctc cacgaaact 
      tgcgatgtt ctcaagaac gtgacaaag gcgtttgga 
      gtcacttta tacatggtt gaccccaac aacgatgaa 
      atttacttg acgaagaaa aaagccagc gaatggccg 
      aaacaccgg tggacaatc gaacaaggg acaattgtt 
      gcggctttc gtcgcttat aaaaaagaa tatgtgatg 
      ttggaggac gtaacgcga gatgaacgc ttccctgaa 
      gggatcgca cggaagggc gaagccgtc aagtcggtg 
      ctttgcgtt cctgtcgtg accccagat ggtgactgt 
      ttggcagtc atcgagttg tatcggcaa tcagaccaa 
      gggcccttc tccaaagac gacctcaaa atcaccatc 
      gtggtcacg gggtggatg ggggctgcc atccaccaa 
      aaccagcaa agggtcgcg ttgaagaaa cagcaagaa 
      ctcaacgag tatctgctc gatttgacc aaaaactac 
      ttctcggac aatgttgtg atggagaaa atgatttcg 
      gacgttgtg aaatttgcg aaaaaaaca ctaggagcg 
      gaaagaggc tcattcttt atcattgat aaagaaaac 
      gaggaactt gttgccgat ctgttcgac gaagggatc 
      gacgaacaa gacacaggt ctttataag aaaaacaca 
      aaaatcagg tttgggaaa gagagaggg attgcggga 
      ctggtggca agaacagga gttgcttgt aacatcaaa 
      gatgcctac aacgattcc agatttaat aaggaaatc 
      gaccaaaag accggattc accaccaaa tcgatcctt 
      tgcatgccc ataatggga gttgaaggg attttggga 
      gttgtccaa gttgtcaat aagataaat ggcggatat 
      ttcacaaac actgacgag gaccttttc aaaactttt 
      tccgtttat tgcgccctt gctttacat tacacgaaa 
      gtcaaccaa caaatgaac aaaatgatg cacgttagt 
      aacatttcg ctgaaattg ctccgactt cagattaaa 
      ccgtgcagt cacgacatc gaagagttt attcaacac 
      ccggacccc aaaataagt ccagatttt taccagttt 
      agttggtac attaatctc aatagtttc gatcaaatg 
      ccgcagtat tgcctctat atgatactg caagtggtg 
      cacgtttcc gaaatcaat atcaaaaat atgatgcag 
      tttatttta tcggccaga aaattatac aggaataac 
      ccttatcac aattttgag cacgctttc aatgtgtgt 
      cactgcatg catgcgatt ttgaggcgc aacttgaaa 
      aaattttca gttgttgag ttgaaagcg ttgataata 
      gcggcatta tgccacgat ttggaccac ggaggcttc 
      actaataat tttctccaa ctaatcaat gatgattta 
      tcgcaactt tacgacgaa tcctttttg gaaaatcac 
      cactttcgg gtcacaatg acgattcta aatgactgc 
      gaaatttac agtaattta tcggaaacc acgtacaaa 
      ataatctcc aaagagata aaagaggcc attctggca 
      acagatctg gcgttgtat ttcaaattt agagcaaag 
      ctggcccag atatgcaac gacagtctt tttgactgg 
      ggcaatcca gtgcatcgg aattttgtt aaagctata 
      atgatgaca agttgcgat ttgtcgggg caatgtaag 
      ccgttttat gtggcgaaa agaatcacc gataacttg 
      tatcgcgaa ttttaccat caaggagac atggaaaaa 
      aagatgggt ttgcatcca ttgtccata atggacagg 
      gaaaaacag catactatt cccgaggat caggtgcag 
      tttttaagc atcgttgtt atcccatgt gtggattta 
      ttaagggtt gttttgccc aatactgag gacttgtat 
      aatgaggct tgcacgtta cgtgacact tggcaggaa 
      atgattcac aagagaggg acaacaaca ccaacgaaa 
      taa
    mRNA sequence
    • >TC032067 RA
      tatttttta aaattatac tttgacgtt tgtcacgta 
      ttttttttc atcttcaag tttgtccaa aaatgccta 
      aagtccagc ccaaagtgg cgaaaaaga taatcaccg 
      gccatgcca ctgaataca gcaataagc cccagatcg 
      tggctgccg atctgccaa ttagtgata gagctacaa 
      agagagtca tcgctccgt ttgtccgaa tcgtccgaa 
      agggacaaa agccgcacg aagctgaag aaagcaaac 
      attttagtt tttttcttc aaaggaaac cgagtgttc 
      taatccgcg acctatcct cctttttat ccgatagtg 
      tcgatctgc catctttac tacacgaaa ctgccgacg 
      tcttgaaaa gcataacga acgcagcgg gagttactt 
      tatacatga tggacgctg caactaacg aaatttacc 
      aaagtaaca aattcccgc agcgggacc gctacaaga 
      tcaggtgga aaatccaag aagggacaa ccacagcag 
      tttatgtgg cgtacaaaa aaaactacg ttttgattg 
      atgatatta ttgaagacg accgctttc ctgaaggga 
      tcggatacg aaactgaga ctattaagt cggtgcttt 
      gcgtccctg ttgtaacac ccgatgggg aatgtttcg 
      cagttattg agctctacc gtcaattat ttgacgagc 
      cgttctcaa aagatgacc tcaaaatca ccctcattg 
      tcacagggt ggatggggg ctgctatcc accaaaact 
      cgcgtagac ttgccctcc aacgccaac aagaactca 
      acgactatc ttctagatt tgactaaat gcttctttg 
      gcgagtcgg ttgtgatgg aaaaaatga tttcagaaa 
      tagtcaatt tcgcaaaag tgactctta acgctgaga 
      aaggaacaa tttttatta tcgatcggg acagtggag 
      atgatcttg ttgccgata tgtacgatg ataccgagg 
      acggaaaag aattcaaaa aagcacaaa aagtgcgtt 
      tttcgaaag agcgcggaa ttgcgagtc ttgtggccc 
      gatcaggga tcacagtca atgttaaag atgcgtata 
      aggatgcac gtttcaata aggaaattg atcagaaaa 
      caggattta tcacaagat caattctgt gtatgccta 
      tagtcggag tagaaggaa ttttaggag tagtacaag 
      ttgtaaata agaaaagag gtggtcatt tcacaagta 
      ctgacgaat cgttgttta gaaccttct gtgtgtatt 
      gtgcacttg ctttgcgat tcactaaag tgcaaaagc 
      ggatgcagt caacgagac tgttgaata attgtttcc 
      tgaagatga tccaactgc aagtgaaac cttgcatcc 
      acgatatca tcacataca acgtggcaa actcacaag 
      tggtgatcc ccatggatt ttgaccact ttgggtggc 
      acattaata cagaagatc acccgaaaa tgccccagt 
      atatacttt acatgttcc tccaattga ttaaaaata 
      cactacatc ctgaacgtt gcatccact acatccttt 
      cggtgaaaa aattctaca ggcggaatc cttaccaca 
      acttcgagc atgcgttca atgtagtcc attgcatgt 
      atcttatat tgaaacgaa acatcaaac aatttactg 
      agactgaaa ttaaagcct taatgattg ctgctctct 
      gccacgatg tggaccatc caggtttca cgaataatt 
      tcctcgagt tgagtaaca acgatttgg ccaatttgt 
      acgaaaatt cgtgtttgg aaaaccacc agtattacg 
      tggctatgt tgttactcg accataata aagttctga 
      cacaccttt caaaagagg agtacgagg ttttgagga 
      cagaaatca aacaagcaa tcatcgcaa ctgatcctt 
      tggtttact ttgcgaacc aaatcaatt tggagaaga 
      tttgcaata cgcattcgt ttgactggg gtaatgaga 
      cacaccgtt attatttga aagggacca tgatgatgg 
      ttgctgatc tctcgggga tgtgtaagc cgtttctgg 
      tgtcgaaac gaattacgg acaatttat atgccgagt 
      gttacttgc aaggggatt tggagaagg aaatggggc 
      tttgtccct tatctatga tggataggg acaagagca 
      attttattc cagaggatc aagttactt ttataacca 
      ctgtgatac tcccttgtt tggacattt tggcccaac 
      ttttgccaa acactgagg cgttataca gcgacacca 
      aattattgg tccaaaact ggcaagata ttatcgacg 
      tcagaggca agaaatgct ggcgccagg acgactcta 
      ttttatcac acctcccta agtcccccc gaacccact 
      tttatcgcc aaacatttc aagcaaaag acgacaagc 
      tcttgagct tcttcctgc agaaaaccc caagtgtgc 
      ttttacgcg agctttcgt cctttctga ctgacagtg 
      ttgaccttc cgtctcttc tccacgaaa cttgcgatg 
      ttctcaaga acgtgacaa aggcgtttg gagtcactt 
      tatacatgg ttgacccca acaacgatg aaatttact 
      tgacgaaga aaaaagcca gcgaatggc cgaaacacc 
      ggtggacaa tcgaacaag ggacaattg ttgcggctt 
      tcgtcgctt ataaaaaag aatatgtga tgttggagg 
      acgtaacgc gagatgaac gcttccctg aagggatcg 
      cacggaagg gcgaagccg tcaagtcgg tgctttgcg 
      ttcctgtcg tgaccccag atggtgact gtttggcag 
      tcatcgagt tgtatcggc aatcagacc aagggccct 
      tctccaaag acgacctca aaatcacca tcgtggtca 
      cggggtgga tgggggctg ccatccacc aaaaccagc 
      aaagggtcg cgttgaaga aacagcaag aactcaacg 
      agtatctgc tcgatttga ccaaaaact acttctcgg 
      acaatgttg tgatggaga aaatgattt cggacgttg 
      tgaaatttg cgaaaaaaa cactaggag cggaaagag 
      gctcattct ttatcattg ataaagaaa acgaggaac 
      ttgttgccg atctgttcg acgaaggga tcgacgaac 
      aagacacag gtctttata agaaaaaca caaaaatca 
      ggtttggga aagagagag ggattgcgg gactggtgg 
      caagaacag gagttgctt gtaacatca aagatgcct 
      acaacgatt ccagattta ataaggaaa tcgaccaaa 
      agaccggat tcaccacca aatcgatcc tttgcatgc 
      ccataatgg gagttgaag ggattttgg gagttgtcc 
      aagttgtca ataagataa atggcggat atttcacaa 
      acactgacg aggaccttt tcaaaactt tttccgttt 
      attgcgccc ttgctttac attacacga aagtcaacc 
      aacaaatga acaaaatga tgcacgtta gtaacattt 
      cgctgaaat tgctccgac ttcagatta aaccgtgca 
      gtcacgaca tcgaagagt ttattcaac acccggacc 
      ccaaaataa gtccagatt tttaccagt ttagttggt 
      acattaatc tcaatagtt tcgatcaaa tgccgcagt 
      attgcctct atatgatac tgcaagtgg tgcacgttt 
      ccgaaatca atatcaaaa atatgatgc agtttattt 
      tatcggcca gaaaattat acaggaata acccttatc 
      acaattttg agcacgctt tcaatgtgt gtcactgca 
      tgcatgcga ttttgaggc gcaacttga aaaaatttt 
      cagttgttg agttgaaag cgttgataa tagcggcat 
      tatgccacg atttggacc acggaggct tcactaata 
      attttctcc aactaatca atgatgatt tatcgcaac 
      tttacgacg aatcctttt tggaaaatc accactttc 
      gggtcacaa tgacgattc taaatgact gcgaaattt 
      acagtaatt tatcggaaa ccacgtaca aaataatct 
      ccaaagaga taaaagagg ccattctgg caacagatc 
      tggcgttgt atttcaaat ttagagcaa agctggccc 
      agatatgca acgacagtc tttttgact ggggcaatc 
      cagtgcatc ggaattttg ttaaagcta taatgatga 
      caagttgcg atttgtcgg ggcaatgta agccgtttt 
      atgtggcga aaagaatca ccgataact tgtatcgcg 
      aattttacc atcaaggag acatggaaa aaaagatgg 
      gtttgcatc cattgtcca taatggaca gggaaaaac 
      agcatacta ttcccgagg atcaggtgc agtttttaa 
      gcatcgttg ttatcccat gtgtggatt tattaaggg 
      ttgttttgc ccaatactg aggacttgt ataatgagg 
      cttgcacgt tacgtgaca cttggcagg aaatgattc 
      acaagagag ggacaacaa caccaacga aataaatgt 
      acaaaacag tcttgatat aaataaatt tatttgagt 
      aaa
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