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Gene information
General information
TC030619 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 6071159..6102399
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.

    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.

    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02646

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 6 (9 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection

    RNAi sequence
    Left: Right:
    Protein sequence
    • >TC030619 RA
    CDS sequence
    • >TC030619 RA
      atgccgcgt ttcgaccag tcctttacc ggtctctgc 
      gagcaaaat cttcaagac accctgcag ctgaagcag 
      cgctatttg gacgccaaa gcgaaaaga gtaccgaaa 
      agtaaagac aaaaagcag caagaatcg attcaaagc 
      agcgtgcac gtgcagcag aaattcttg aaacgatcg 
      gcggaagag cacgatccg ggagggtca tctgagaat 
      ttcgagcca ccggtgaaa ctccagtgc acaacacag 
      cagcacggt tcgcatcag aacgaaggt ctgacgaag 
      ttttcggtc gaaatcgtc cagcagctc gaattcaca 
      acgtcggcg gccaattcc cagcctcag cagatctcc 
      accaacgtg acggtgaag gcgcacacc aacgcctcg 
      gtgaagagc gacgtctca tcgcccaag tcgaatcct 
      tcgacgccg gcgtccaac ctccagcag aagtccaac 
      atcggcctc gacgtcggc actttggtc gagtgcgtc 
      aagcaggag cccgacaac gatttcgcc gatttagac 
      caatgtgcg gcggcgtta gagaaggat gcggccgcc 
      aacggtggc accggcttt ggtggtttc tccgacctc 
      atcggcgaa gacaccaac gacgagatc atcacgtct 
      gacgccttc aacgatctc atctcagat attagcaac 
      tatcccgag ttaatgaaa gactttgat ttcgaagat 
      aaatccgag gttaacgcg agtgttaac ggtttgaaa 
      gtggaagac attaaagat ctgcaacaa ccggtggca 
      gacaacgcg aaatccact cacagtcct ttagtttcc 
      aatcaaaat aactattcc ccagtcttc gaaaaccaa 
      ggaggcatc aacaaaacc cgcatgcct ccttacact 
      aacattgat ttctgcaag actgaactg agtccggcc 
      gcacaaacc ctcaagcaa atggccgaa caacaccaa 
      cataaaaat caaatgggg atgactttc aaccccaac 
      accagagcg ccgaattcc aggtcacct taccccgat 
      ttccagttc caaggtgac tatggaagc cccaactcg 
      aaccccaac ttccataaa aacagtccg aatttctcg 
      caacctgac atgatcaag caggagatg atcttccaa 
      gggaacgat tttgacatg aagcggaaa ggtgcaggg 
      atttacaaa cagcagcaa tattcccct tatagcagt 
      cccagtgcg agtagtcac ggcagtccg ggttattta 
      ccgagaagt ggtaccgga tcgacaagt ggtggcgcc 
      ttcggcggc ggtactccc ccgagaccg ccctcggga 
      cctggtggc aacaattcg ggaaccacc ttgcagata 
      aatcaagcg caacaactc catataagt cagcagaat 
      cacggacac gccattcag gtgtcggct ggtcaacac 
      cttcattta agcggcgac ttgaaaggc aatgtatcc 
      atcgcgaca caacaagga atgcacttt aatcaacac 
      acgacaaca aatggtacc tcgtctcaa tcccagaac 
      ggacaaaac gttccccaa cagaacccg ctctcatca 
      aaccaacaa caacagaac caacaacaa atgcacaac 
      agcaatcaa caacaaccg caaaaccca acgatgggc 
      agtcccatg atgtccaat aaccaacaa atgccgaat 
      tcgcaacaa ggctcaatg ggcggaatg caagggatg 
      atgggtggt ccaggaatg caacatcct gggacgatg 
      ggtggcaac ttatcagga caaatgcca gacggctac 
      tccatgtca caaagccag accatcaac ttcacccag 
      caaacctta aggcaacgt gccaatgga cccaatgtc 
      gtaccaaat aatatggga ccaaatggg ccaatggga 
      ccaggacct atgggaggt caaggcgga atgggacca 
      cgtctaatg aatactcaa ctcgaacaa cagaaactt 
      ctgcaacaa cagcaaatg atgcgcgtg caacaagcc 
      gcactgcaa cagcatatg gtcaggccg ccgcccccg 
      gattacaag agtagtgca ggaatgatg caaggtgtg 
      caacccaga tttgcagga gcgccacct aacatccga 
      agaatgcca caacagccg atgccaccg tcaggtcca 
      atgatacga ccacataat atgtacgtg atgcaacag 
      caccaacag caacagcac atgatctcc agaaatatg 
      tacccgcga cagcaagga ccactcagt ggaatggac 
      gcaatgcaa catggaaac accgagtgg cgacatctg 
      ctgatgcaa caacagaac actaatttt aatccgcag 
      atgaggcca aacatgcaa caaggtttt aacatgaac 
      gccggaaac atgcagctg tcagcccag caccagcag 
      atcagaagt cagcagagc atgggtcag gcccaggtg 
      atgggccaa agtggcagt cccatgtcg caaatgaac 
      aacatgaac caaatgttg aaccacatg cagcaacag 
      cagagtatg atgcagcaa cagcagcag cagcaacaa 
      caacagcaa cagaattct cagatgtcc atatcaagc 
      atccacatg caacaaacc cagtcgatg acagtgacc 
      aatcagaca ctccagcag aacatgaac aatcaactt 
      gtaaataat tcatcaagc aaccaaaat aattcgtta 
      aataatttc aattcccaa tcgacggac tttatcggt 
      ctcgaattt ctagataat cttccaaca actgattca 
      actgcattt actgatcag gagctgcta agctcgttt 
      gacagtgac tctggcttt aatttagat ttttag
    mRNA sequence
    • >TC030619 RA
      acaggtggc tgcactggc acgccattc gttgtaaaa 
      tggccgcgg tgtgtgtgt taaactcga gagtgaaag 
      tgtgtttgt gtttcgtat tgaaataaa ctgttgtga 
      aacactgag aaacaattg ttttaaaag cgattgtgt 
      cgaaagaga ttgtctgtg aagttttgg tcgtcggtg 
      cgtcggtta caacaagcc ccgagtcaa aagagccca 
      acttggctc cagtcgcag tgtttataa cctcaacgc 
      cacttaaac tggactttt cgagtcctc aaagagtaa 
      acaaacaaa gtttcattc atcagtttg gggaacgga 
      gcaccggga ggaaacgcc atgcgttcc aaaacaacc 
      tgttaaaaa agtccactg aactttgtg tttacatcc 
      tgacggaga gcccctcgc cgctgcgct gcctccagc 
      gagtcaaag gggccccga gatgcacac ctatgtccc 
      ttacgtgtt aaaatgcac atggataca acgcaaaac 
      atcagttaa ccttcagca ctcaattga gaaggcaag 
      ctgtaccta aacactgat tgacacgtc tggagatac 
      aaatttgaa ggtcgttta tctaaataa aatgtgttt 
      tgccatcgt gcagttggg tgcgagact gttttgaac 
      tacccgcgt caagtcatg gtcggttca ggagtgtga 
      aattattta tttacaagt gcgatgtaa aaacggaca 
      cgcagatcg acacactgg atcaagcca gctctggtc 
      cagggggtt gccacgacg gtggaggga cagtccccc 
      ccgtcgtcc cacagcccc cgggactgt cgccctttc 
      ctcacgaaa tcaacactc ccaagcgac aagctgtgg 
      tagaaagac ttcgaagaa gaatcgaaa attatcgaa 
      gaagacaga acgactgca tgccgcgtt tcgaccagt 
      cctttaccg gtctctgcg agcaaaatc ttcaagaca 
      ccctgcagc tgaagcagc gctatttgg acgccaaag 
      cgaaaagag taccgaaaa gtaaagaca aaaagcagc 
      aagaatcga ttcaaagca gcgtgcacg tgcagcaga 
      aattcttga aacgatcgg cggaagagc acgatccgg 
      gagggtcat ctgagaatt tcgagccac cggtgaaac 
      tccagtgca caacacagc agcacggtt cgcatcaga 
      acgaaggtc tgacgaagt tttcggtcg aaatcgtcc 
      agcagctcg aattcacaa cgtcggcgg ccaattccc 
      agcctcagc agatctcca ccaacgtga cggtgaagg 
      cgcacacca acgcctcgg tgaagagcg acgtctcat 
      cgcccaagt cgaatcctt cgacgccgg cgtccaacc 
      tccagcaga agtccaaca tcggcctcg acgtcggca 
      ctttggtcg agtgcgtca agcaggagc ccgacaacg 
      atttcgccg atttagacc aatgtgcgg cggcgttag 
      agaaggatg cggccgcca acggtggca ccggctttg 
      gtggtttct ccgacctca tcggcgaag acaccaacg 
      acgagatca tcacgtctg acgccttca acgatctca 
      tctcagata ttagcaact atcccgagt taatgaaag 
      actttgatt tcgaagata aatccgagg ttaacgcga 
      gtgttaacg gtttgaaag tggaagaca ttaaagatc 
      tgcaacaac cggtggcag acaacgcga aatccactc 
      acagtcctt tagtttcca atcaaaata actattccc 
      cagtcttcg aaaaccaag gaggcatca acaaaaccc 
      gcatgcctc cttacacta acattgatt tctgcaaga 
      ctgaactga gtccggccg cacaaaccc tcaagcaaa 
      tggccgaac aacaccaac ataaaaatc aaatgggga 
      tgactttca accccaaca ccagagcgc cgaattcca 
      ggtcacctt accccgatt tccagttcc aaggtgact 
      atggaagcc ccaactcga accccaact tccataaaa 
      acagtccga atttctcgc aacctgaca tgatcaagc 
      aggagatga tcttccaag ggaacgatt ttgacatga 
      agcggaaag gtgcaggga tttacaaac agcagcaat 
      attcccctt atagcagtc ccagtgcga gtagtcacg 
      gcagtccgg gttatttac cgagaagtg gtaccggat 
      cgacaagtg gtggcgcct tcggcggcg gtactcccc 
      cgagaccgc cctcgggac ctggtggca acaattcgg 
      gaaccacct tgcagataa atcaagcgc aacaactcc 
      atataagtc agcagaatc acggacacg ccattcagg 
      tgtcggctg gtcaacacc ttcatttaa gcggcgact 
      tgaaaggca atgtatcca tcgcgacac aacaaggaa 
      tgcacttta atcaacaca cgacaacaa atggtacct 
      cgtctcaat cccagaacg gacaaaacg ttccccaac 
      agaacccgc tctcatcaa accaacaac aacagaacc 
      aacaacaaa tgcacaaca gcaatcaac aacaaccgc 
      aaaacccaa cgatgggca gtcccatga tgtccaata 
      accaacaaa tgccgaatt cgcaacaag gctcaatgg 
      gcggaatgc aagggatga tgggtggtc caggaatgc 
      aacatcctg ggacgatgg gtggcaact tatcaggac 
      aaatgccag acggctact ccatgtcac aaagccaga 
      ccatcaact tcacccagc aaaccttaa ggcaacgtg 
      ccaatggac ccaatgtcg taccaaata atatgggac 
      caaatgggc caatgggac caggaccta tgggaggtc 
      aaggcggaa tgggaccac gtctaatga atactcaac 
      tcgaacaac agaaacttc tgcaacaac agcaaatga 
      tgcgcgtgc aacaagccg cactgcaac agcatatgg 
      tcaggccgc cgcccccgg attacaaga gtagtgcag 
      gaatgatgc aaggtgtgc aacccagat ttgcaggag 
      cgccaccta acatccgaa gaatgccac aacagccga 
      tgccaccgt caggtccaa tgatacgac cacataata 
      tgtacgtga tgcaacagc accaacagc aacagcaca 
      tgatctcca gaaatatgt acccgcgac agcaaggac 
      cactcagtg gaatggacg caatgcaac atggaaaca 
      ccgagtggc gacatctgc tgatgcaac aacagaaca 
      ctaatttta atccgcaga tgaggccaa acatgcaac 
      aaggtttta acatgaacg ccggaaaca tgcagctgt 
      cagcccagc accagcaga tcagaagtc agcagagca 
      tgggtcagg cccaggtga tgggccaaa gtggcagtc 
      ccatgtcgc aaatgaaca acatgaacc aaatgttga 
      accacatgc agcaacagc agagtatga tgcagcaac 
      agcagcagc agcaacaac aacagcaac agaattctc 
      agatgtcca tatcaagca tccacatgc aacaaaccc 
      agtcgatga cagtgacca atcagacac tccagcaga 
      acatgaaca atcaacttg taaataatt catcaagca 
      accaaaata attcgttaa ataatttca attcccaat 
      cgacggact ttatcggtc tcgaatttc tagataatc 
      ttccaacaa ctgattcaa ctgcattta ctgatcagg 
      agctgctaa gctcgtttg acagtgact ctggcttta 
      atttagatt tttagtaga attttatat aatttttgt 
      taatatttt ttatatata tatttttgt acatattgt 
      actgtggtt tctgttggt ctttagttt ttttggtta 
      caatgcttg aaatttgtt tttgataca acgtttaca 
      agaaatgaa cccgaatga caatatacc tatcaaata 
      tctacagtt tgtattaaa ttacactgc catgaagca 
      gattatgat tataaaata gagctgtga caaagtgga 
      gtgaaattt tgtagatat cagctgcaa aacagaatt 
      tacatttat acagtttag agaagtgta cacaaaatt 
      gaaactgaa tctcatctg tcatatcac gacttgtat 
      acacaattg ataagtatt tatatcgtg ttatatttt 
      actcctaac cgtaggaag ttggcgtac agtacgtaa 
      tatttacga tcgtgaaac taacggcag aaaatcgta 
      aatattgca agtggcggt gactagttg aaataaata 
      taattagag aacagttct ttaaactcc tgtaatcca 
      ttagtaaat gttcgagca tccgttact tgcagctta 
      tttggtaat tgtgtggcg tgtcgttgt gtctaaaat 
      ttgtcattt cgcattttt gtaacgttc taaattcct 
      aacggtagc tacttgcga tgcgctcca agattaccg 
      aatacctag ccaaagtcg gtgttctta gtgaaataa 
      ttatgtaat gttactggc ttaaatata agatttgat 
      ttttcaagt ttggttggt gaaatattg tcacatgca 
      aagactatt tttttattc atgtttgat atttaagcc 
      actaaattg aggacgatg atctcaata aattttaat 
      tagtgaaaa attgcgttt ctttctatg atgatgact 
      aggatatac attccaaag tgtaattct gtttccaaa 
      gttttgtaa aaaccttct ctcagtatt gaattgctt 
      atttcttct gcacttatt agcagacca cagtcttag 
      ttttatctg taataattt atacgtgta cttagatga 
      aatgacatt gttatcata ttctaattt ttgcaacat 
      acaagagtt tatattgaa aatatatat tccatgttt 
      tgtagattt atcataaat tcaggtgat tataataaa 
      tatccataa tgaca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY