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Gene information
General information
TC000808 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 454636..554043
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000808
    iBeetle screen iB_02645
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    larva segment partiallynot present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000808 RA
      MEFPAVLLT LLLSKAVIS LGEDCTTGF SVVAPELAV 
      PGKTTAVLV TLHGPTSLR PLNVTLRLL PDAQKGTNQ 
      QLVETTQEI RGHGILPLE VPLEASGSY VLQTLVNCT 
      GNDRCELQS SSQMRLVGP VRDVIIRPA KHAYRPGET 
      VSFWVLALD HDLQIASGA IGSVSIKDP AGTKVALWD 
      QVALDEGVK AFTLPLSEN VNSGRWLLH VEVESAEFS 
      APIEIAPGA GNGLPDVAA AEEHYVELR FGREMRRSY 
      KPGLPFAGK VEAMSTEKS VRVRVKVYD NTTSIYSQD 
      IEISNGEGT FVVPAILAD SNIIALQAE LVSVDSKEI 
      ESHYVLARE PIHKWNSSS DCYLLIEGV EHTLQPEEE 
      AHVAILSTC PCERDLHYV ITTDGRVTD WAQRRYEDP 
      VPPATSASA GAVCRLNFS FTVRSVMAP VSQLLVYYV 
      TPQGEPVSD VISFDVKLL HRQVYVNLE EREYWLPGQ 
      SLDLEVVAE RSSLVCLLG GRSGGNGDV KFDPRISEE 
      REPATVAGE IDFLEAGVS FFQRQCVKR GNSGISAVS 
      YRQRGSGAG PSNRRRPPE SLVGGAPFD QLWLWKCFN 
      YSSEIASTG LTISAPQEA GKWSLWALT VSSNGLRFS 
      SSVNVQVFR PLQAEFHLP PSLRVRETL EVDIKIGNN 
      INSCMDVTA LLALSEGAQ FLSNGLLYV TERLRLGPH 
      GATSLVVRL LVTTPGVKN MTVEVNGYT SPSCEGTEK 
      PGNTSLAGA VLRSATIKV YPEGIVRTD TESAYFCAN 
      ENLVISTAD KYKYEWVAA PRNHEGIVL ELKAGVSKR 
      IGPIHIALA ESRSPSDKM YRLTIGDVN NMITWIGRG 
      KHGYGVQLT RVETPGILS EDEWCTFWI TWEKNTIAF 
      GNGSIPHNN TLLKWRMDK KIKIQQVGF ASSWGSSAE 
      FRIWNFNEE AGFSQVLHL DTPKSVVPG SEWGKLLVA 
      GGLALPAIN KKIEGGGLS GALAALTPL LTLQHLGKK 
      ANKTERLQA LQSLPGDIQ DILSYRKVD NSFSEHQML 
      GSHTATVSI LEALTKVQS YFNIDPDLI QAIKRWVQL 
      RQEDDGRFS PLPADVKFP SSKQTDFMR KNLSSEIAL 
      LEHVIEITA DTVVTLYEI EIENDADSE TLQKAKIFL 
      ENSLPKLQT PETIAVVAL ALVLVRSAT ASWAIEKLR 
      NVSTTEDGE FGWPHLVPR RDAADWLYE SESEKTLKE 
      PLATTLDEY KASLHALST FCIIGDLKF AESVARYLF 
      YRSHMLDKH PELLYQAVK TFTQYDLLA KDRHRALTV 
      SLATSGMEL TDTLELKPD KPPQSLHLP SLPTKVFVY 
      ATGAGCATI QGKVSYSTY SVKRATPLL ELWSGVVQE 
      ILPDRSSVE EIEGKLPML KLKTCFRWK GKTPSGILR 
      LEISLFSGF ELATTPPQL LNPPEDMSE MQHNFHDNK 
      LWFIFANIS TLCPVCVQY MARSAFVIS SLRPAYAKV 
      YPAGRQDLA AETFFHTKI GSNLLKSIT EDDLITWFG 
      KNRTDISEP YFQFPQNCI REVSTTTST TTISTTTDN 
      PPVPSPKLE VLSVATYTI NVDNQSISN INDTEKHKS 
      TATPMIEEN AIPHHEEEK TLKKKSRSI TGKNLQSTT 
      VPDHVVTES ITTVSTPSE VTTKLAEKE LVVDKNVSP 
      PRHVNTVIH NSPIKYIEK DAKHSEESL ATFAPEIKN 
      DQYVLLDKE ELWGMLKEV VDDQLKKKT SSKIVDGEK 
      LRSQGFT
    CDS sequence
    • >TC000808 RA
      atggagttt cctgctgtc ctgctgaca ctcctgctg 
      agcaaagct gtaatttcc ttaggagaa gattgcact 
      acaggcttt tctgtagtg gctcccgaa ctagccgta 
      cctggaaaa acgacagct gttctggta actctacac 
      ggtccaacg agtctccga cctctcaac gttaccctc 
      cggctcctt ccagatgct caaaaaggg acaaaccaa 
      caactcgtt gaaaccact caggaaata agagggcac 
      ggcatcttg cccttggag gtaccatta gaagcgtca 
      ggaagctac gtgctccag acattagta aactgcacc 
      ggaaacgac cgctgcgaa ctacaaagc tcgtcccaa 
      atgagactt gtcggacct gtcagggac gtcattatt 
      cgtcctgcc aagcatgcc tacaggcct ggagaaacc 
      gtgtctttc tgggtctta gcgctggat catgatttg 
      cagatcgcg tctggagct attggaagt gttagcatt 
      aaagaccca gctggcact aaagtcgca ctttgggat 
      caagtggct ttagatgaa ggagtgaaa gcgttcaca 
      ttgccgttg tcggagaac gtgaattct ggcaggtgg 
      ttgcttcac gtcgaggtg gaatctgcc gaattttcc 
      gcacctatc gagatagct ccgggtgcg ggaaatgga 
      cttcctgat gttgcagcc gccgaagag cattacgtt 
      gaattgcgt tttgggagg gaaatgagg cgcagctat 
      aagccaggc ttaccgttc gctggaaag gtggaagca 
      atgagcacg gaaaaatcg gttcgtgtc cgtgtcaaa 
      gtgtacgac aacaccact tcaatatac agccaggat 
      attgaaata tccaacggt gaaggcact tttgtcgtt 
      cccgctatc ttggctgac agcaatatt attgcattg 
      caggcggag ctagtatca gtcgacagt aaagaaatt 
      gaaagccat tacgtgctg gcgcgggaa ccaatccat 
      aaatggaac tcatcttcg gattgttat ctcctgatt 
      gaaggggtc gagcacacc ctccagccg gaagaggaa 
      gctcacgtc gccatcctc tccacgtgt ccttgcgaa 
      cgtgacctg cattatgtc atcaccacc gacggccgg 
      gtcaccgac tgggcgcag cggcgctat gaagaccct 
      gttcctccc gccacttcc gcttccgca ggagccgtt 
      tgtcgtctt aatttcagc tttacggta cgatctgtg 
      atggctccc gttagtcaa cttctcgtt tactatgta 
      actccccaa ggggagccc gtcagtgac gtaattagt 
      tttgatgtg aagttgcta cataggcag gtgtacgtg 
      aatttggag gagcgagaa tattggtta cctggacaa 
      tctctggat ttggaagtg gtggcagag cgttcctcc 
      ctcgtgtgt ctgctagga ggtcggtca ggaggcaac 
      ggggacgtg aagttcgac cctcgaatt agcgaagaa 
      agagagccg gcaacggtc gccggcgaa attgacttc 
      ctggaagcg ggagtttcc ttctttcag cgacaatgc 
      gtgaaaagg ggcaattcg ggaatctct gccgtgtcc 
      tacagacaa aggggctcc ggtgcaggg ccgtccaac 
      agaaggagg cctcctgaa agcctggtg ggaggagcg 
      ccgttcgat caactttgg ctctggaag tgctttaat 
      tattcgtca gaaattgct tccaccgga ctgactatt 
      tcggcgcct caagaagcc ggtaaatgg tctctgtgg 
      gctttaacc gtgtccagc aatggtctg agattttct 
      tcgtccgta aacgtccaa gttttccga cctctgcag 
      gcggaattc catttacct cccagtttg agagttcgc 
      gaaactttg gaagtcgac attaagatt ggaaataac 
      ataaactcc tgcatggac gtgacagca ctattagcc 
      ttgagcgaa ggagcgcaa ttcctaagt aacggatta 
      ctctacgtt actgaaagg cttcgcttg ggtccacat 
      ggtgctaca tctttagtt gtcagactc ttagtgaca 
      actccaggt gtgaaaaat atgacagtg gaagtaaat 
      ggttacacg agtccttct tgcgaaggc actgaaaaa 
      ccgggaaac acatctcta gcaggagct gtgcttcgt 
      tcagctaca attaaggtc tatcccgaa ggaattgtg 
      agaacagac accgagagc gcttatttt tgcgccaac 
      gaaaacttg gtaatttcc accgccgac aaatataaa 
      tatgaatgg gtagcggcg ccgcgcaat cacgagggt 
      atagttctg gagctgaaa gccggagta tctaagcga 
      atcggccca attcacatt gcgctcgct gaaagccgc 
      tccccttct gataagatg tatcgctta accatcggc 
      gatgtgaac aacatgatt acatggata ggccgaggc 
      aaacacgga tacggcgtt caattaaca agagtggaa 
      acgccggga atcttatcg gaggatgaa tggtgcacg 
      ttctggatc acgtgggag aagaacact atagctttc 
      ggaaatggc tcgattccc cacaacaat actttactc 
      aagtggagg atggacaag aagattaaa atacaacaa 
      gtgggtttt gcctcgtca tggggaagc tcagcagag 
      ttcagaata tggaatttc aacgaagag gctggcttt 
      tcccaagtt ctacattta gacactccg aaatcggtc 
      gttcctggt tccgaatgg ggaaagttg cttgtagca 
      ggaggctta gctttacca gccataaac aagaaaatc 
      gaaggtggt ggactttcc ggtgctttg gcagcttta 
      actccgctt ttaactttg caacacctt ggcaagaaa 
      gccaacaag accgaacgc ttacaagct ttgcagagt 
      ttaccggga gatatacaa gacatcctg tcgtatcgc 
      aaagtggac aactcgttc agcgagcac caaatgctg 
      ggcagtcac acggccaca gtttcaatc ttggaagct 
      ttgactaaa gtccagagt tatttcaat atcgatccg 
      gatttgatt caagccatc aagcgctgg gtgcaactg 
      cgtcaggaa gatgacggc cgtttctct ccacttcct 
      gcagacgtc aagtttccc tcgtcaaaa cagaccgat 
      tttatgcga aagaactta agttcggag attgctctc 
      ctcgagcac gttatcgaa attaccgcc gacactgta 
      gtcactttg tacgaaatc gagatcgaa aacgatgct 
      gattcggag acactgcaa aaagccaag attttcctg 
      gagaacagc ctcccaaaa ctgcaaact cccgaaacg 
      atcgctgtt gtcgcccta gcactggtt ctagtccgc 
      agtgcaacc gcctcgtgg gcgatcgaa aagctgcgg 
      aatgtgtcc accaccgag gacggcgag ttcggatgg 
      ccacattta gtcccgcga agagatgcg gctgattgg 
      ttgtacgag tccgaatcg gaaaaaaca ttaaaagag 
      ccgttagcg acgactctg gacgagtac aaagcctcg 
      cttcacgct ttatccact ttttgcata atcggagat 
      ttgaaattt gccgaaagt gtagctcgg tatttgttt 
      tatcgatct catatgctc gataaacac cccgagttg 
      ttgtaccaa gctgtgaaa acttttaca cagtacgat 
      ttattggcc aaggatcgg catcgcgct ttaactgtc 
      tcattggca acttcggga atggaactc acagacact 
      ttggaactt aaaccagat aaaccgccg caaagtctt 
      catttgccg agccttccc accaaagtt tttgtctac 
      gccaccgga gcaggatgt gccacaatc cagggaaaa 
      gtatcgtat tcgacatat tccgtgaaa cgcgcgact 
      ccgctcctt gaactttgg tcgggtgta gttcaggag 
      attctcccc gaccgcagt tcagtcgaa gaaatcgaa 
      ggcaaactc ccgatgctt aagctcaag acatgcttt 
      agatggaaa ggaaaaacc ccatcggga atactccgc 
      ttagaaatc tcccttttt tcaggcttc gagctcgca 
      accactcca ccacaatta ctaaatcct cccgaagac 
      atgtccgaa atgcagcat aacttccac gataacaag 
      ctgtggttc atttttgcc aatatctcc actctgtgt 
      cctgtttgc gtccaatac atggcccgc agcgccttt 
      gtgatctca tccctccgt ccagcttac gcgaaagtt 
      tatcccgct ggcagacaa gatctagcc gctgaaaca 
      ttcttccac acgaaaatc ggaagcaac ttactcaag 
      tcgataacc gaagacgat ctaatcact tggtttggc 
      aaaaacaga acggatatt agcgagcca tatttccaa 
      tttccacaa aactgtatt agagaagtt tcgacaact 
      acttccacc accacaata agcaccacc acggataat 
      cccccagtt ccatcaccg aagctggaa gttttaagc 
      gtggccact tacacaatc aatgtcgat aaccagtcc 
      atttcgaat attaatgac acggaaaaa cacaagtcc 
      actgcaact ccaatgatt gaagaaaac gcgatacct 
      caccacgag gaagagaaa actttgaag aagaagagt 
      cggagcatc acgggcaaa aatttgcaa agcacaacc 
      gttccagat cacgtcgtg acagaatcg attaccacg 
      gtctcaact ccgagtgaa gtgacgaca aaattagct 
      gagaaagaa ctcgtggtg gacaagaac gtgtcgccc 
      cccagacat gtaaacaca gtcatccac aacagcccc 
      ataaaatac atagagaaa gatgcgaag cactccgag 
      gagtcttta gcgacattc gctcccgaa ataaagaat 
      gatcaatac gtgctgtta gataaggag gagctctgg 
      gggatgcta aaggaggtt gttgatgac caacttaaa 
      aagaaaacg agttcgaag atagtcgac ggcgagaag 
      ctccgcagc caaggcttc acgtaa
    mRNA sequence
    • >TC000808 RA
      cggtgtgat tgcatctcg cgtcgcgac gccccttca 
      ctgatttac cgattattg ataggagct tgcgagttg 
      tgcactttg cgtcagctg ttgcaactg caaagctcc 
      gctagctta taactggat tgccaagta taatgctgt 
      gaacttgtg gcacataat ggagtttcc tgctgtcct 
      gctgacact cctgctgag caaagctgt aatttcctt 
      aggagaaga ttgcactac aggcttttc tgtagtggc 
      tcccgaact agccgtacc tggaaaaac gacagctgt 
      tctggtaac tctacacgg tccaacgag tctccgacc 
      tctcaacgt taccctccg gctccttcc agatgctca 
      aaaagggac aaaccaaca actcgttga aaccactca 
      ggaaataag agggcacgg catcttgcc cttggaggt 
      accattaga agcgtcagg aagctacgt gctccagac 
      attagtaaa ctgcaccgg aaacgaccg ctgcgaact 
      acaaagctc gtcccaaat gagacttgt cggacctgt 
      cagggacgt cattattcg tcctgccaa gcatgccta 
      caggcctgg agaaaccgt gtctttctg ggtcttagc 
      gctggatca tgatttgca gatcgcgtc tggagctat 
      tggaagtgt tagcattaa agacccagc tggcactaa 
      agtcgcact ttgggatca agtggcttt agatgaagg 
      agtgaaagc gttcacatt gccgttgtc ggagaacgt 
      gaattctgg caggtggtt gcttcacgt cgaggtgga 
      atctgccga attttccgc acctatcga gatagctcc 
      gggtgcggg aaatggact tcctgatgt tgcagccgc 
      cgaagagca ttacgttga attgcgttt tgggaggga 
      aatgaggcg cagctataa gccaggctt accgttcgc 
      tggaaaggt ggaagcaat gagcacgga aaaatcggt 
      tcgtgtccg tgtcaaagt gtacgacaa caccacttc 
      aatatacag ccaggatat tgaaatatc caacggtga 
      aggcacttt tgtcgttcc cgctatctt ggctgacag 
      caatattat tgcattgca ggcggagct agtatcagt 
      cgacagtaa agaaattga aagccatta cgtgctggc 
      gcgggaacc aatccataa atggaactc atcttcgga 
      ttgttatct cctgattga aggggtcga gcacaccct 
      ccagccgga agaggaagc tcacgtcgc catcctctc 
      cacgtgtcc ttgcgaacg tgacctgca ttatgtcat 
      caccaccga cggccgggt caccgactg ggcgcagcg 
      gcgctatga agaccctgt tcctcccgc cacttccgc 
      ttccgcagg agccgtttg tcgtcttaa tttcagctt 
      tacggtacg atctgtgat ggctcccgt tagtcaact 
      tctcgttta ctatgtaac tccccaagg ggagcccgt 
      cagtgacgt aattagttt tgatgtgaa gttgctaca 
      taggcaggt gtacgtgaa tttggagga gcgagaata 
      ttggttacc tggacaatc tctggattt ggaagtggt 
      ggcagagcg ttcctccct cgtgtgtct gctaggagg 
      tcggtcagg aggcaacgg ggacgtgaa gttcgaccc 
      tcgaattag cgaagaaag agagccggc aacggtcgc 
      cggcgaaat tgacttcct ggaagcggg agtttcctt 
      ctttcagcg acaatgcgt gaaaagggg caattcggg 
      aatctctgc cgtgtccta cagacaaag gggctccgg 
      tgcagggcc gtccaacag aaggaggcc tcctgaaag 
      cctggtggg aggagcgcc gttcgatca actttggct 
      ctggaagtg ctttaatta ttcgtcaga aattgcttc 
      caccggact gactatttc ggcgcctca agaagccgg 
      taaatggtc tctgtgggc tttaaccgt gtccagcaa 
      tggtctgag attttcttc gtccgtaaa cgtccaagt 
      tttccgacc tctgcaggc ggaattcca tttacctcc 
      cagtttgag agttcgcga aactttgga agtcgacat 
      taagattgg aaataacat aaactcctg catggacgt 
      gacagcact attagcctt gagcgaagg agcgcaatt 
      cctaagtaa cggattact ctacgttac tgaaaggct 
      tcgcttggg tccacatgg tgctacatc tttagttgt 
      cagactctt agtgacaac tccaggtgt gaaaaatat 
      gacagtgga agtaaatgg ttacacgag tccttcttg 
      cgaaggcac tgaaaaacc gggaaacac atctctagc 
      aggagctgt gcttcgttc agctacaat taaggtcta 
      tcccgaagg aattgtgag aacagacac cgagagcgc 
      ttatttttg cgccaacga aaacttggt aatttccac 
      cgccgacaa atataaata tgaatgggt agcggcgcc 
      gcgcaatca cgagggtat agttctgga gctgaaagc 
      cggagtatc taagcgaat cggcccaat tcacattgc 
      gctcgctga aagccgctc cccttctga taagatgta 
      tcgcttaac catcggcga tgtgaacaa catgattac 
      atggatagg ccgaggcaa acacggata cggcgttca 
      attaacaag agtggaaac gccgggaat cttatcgga 
      ggatgaatg gtgcacgtt ctggatcac gtgggagaa 
      gaacactat agctttcgg aaatggctc gattcccca 
      caacaatac tttactcaa gtggaggat ggacaagaa 
      gattaaaat acaacaagt gggttttgc ctcgtcatg 
      gggaagctc agcagagtt cagaatatg gaatttcaa 
      cgaagaggc tggcttttc ccaagttct acatttaga 
      cactccgaa atcggtcgt tcctggttc cgaatgggg 
      aaagttgct tgtagcagg aggcttagc tttaccagc 
      cataaacaa gaaaatcga aggtggtgg actttccgg 
      tgctttggc agctttaac tccgctttt aactttgca 
      acaccttgg caagaaagc caacaagac cgaacgctt 
      acaagcttt gcagagttt accgggaga tatacaaga 
      catcctgtc gtatcgcaa agtggacaa ctcgttcag 
      cgagcacca aatgctggg cagtcacac ggccacagt 
      ttcaatctt ggaagcttt gactaaagt ccagagtta 
      tttcaatat cgatccgga tttgattca agccatcaa 
      gcgctgggt gcaactgcg tcaggaaga tgacggccg 
      tttctctcc acttcctgc agacgtcaa gtttccctc 
      gtcaaaaca gaccgattt tatgcgaaa gaacttaag 
      ttcggagat tgctctcct cgagcacgt tatcgaaat 
      taccgccga cactgtagt cactttgta cgaaatcga 
      gatcgaaaa cgatgctga ttcggagac actgcaaaa 
      agccaagat tttcctgga gaacagcct cccaaaact 
      gcaaactcc cgaaacgat cgctgttgt cgccctagc 
      actggttct agtccgcag tgcaaccgc ctcgtgggc 
      gatcgaaaa gctgcggaa tgtgtccac caccgagga 
      cggcgagtt cggatggcc acatttagt cccgcgaag 
      agatgcggc tgattggtt gtacgagtc cgaatcgga 
      aaaaacatt aaaagagcc gttagcgac gactctgga 
      cgagtacaa agcctcgct tcacgcttt atccacttt 
      ttgcataat cggagattt gaaatttgc cgaaagtgt 
      agctcggta tttgtttta tcgatctca tatgctcga 
      taaacaccc cgagttgtt gtaccaagc tgtgaaaac 
      ttttacaca gtacgattt attggccaa ggatcggca 
      tcgcgcttt aactgtctc attggcaac ttcgggaat 
      ggaactcac agacacttt ggaacttaa accagataa 
      accgccgca aagtcttca tttgccgag ccttcccac 
      caaagtttt tgtctacgc caccggagc aggatgtgc 
      cacaatcca gggaaaagt atcgtattc gacatattc 
      cgtgaaacg cgcgactcc gctccttga actttggtc 
      gggtgtagt tcaggagat tctccccga ccgcagttc 
      agtcgaaga aatcgaagg caaactccc gatgcttaa 
      gctcaagac atgctttag atggaaagg aaaaacccc 
      atcgggaat actccgctt agaaatctc ccttttttc 
      aggcttcga gctcgcaac cactccacc acaattact 
      aaatcctcc cgaagacat gtccgaaat gcagcataa 
      cttccacga taacaagct gtggttcat ttttgccaa 
      tatctccac tctgtgtcc tgtttgcgt ccaatacat 
      ggcccgcag cgcctttgt gatctcatc cctccgtcc 
      agcttacgc gaaagttta tcccgctgg cagacaaga 
      tctagccgc tgaaacatt cttccacac gaaaatcgg 
      aagcaactt actcaagtc gataaccga agacgatct 
      aatcacttg gtttggcaa aaacagaac ggatattag 
      cgagccata tttccaatt tccacaaaa ctgtattag 
      agaagtttc gacaactac ttccaccac cacaataag 
      caccaccac ggataatcc cccagttcc atcaccgaa 
      gctggaagt tttaagcgt ggccactta cacaatcaa 
      tgtcgataa ccagtccat ttcgaatat taatgacac 
      ggaaaaaca caagtccac tgcaactcc aatgattga 
      agaaaacgc gatacctca ccacgagga agagaaaac 
      tttgaagaa gaagagtcg gagcatcac gggcaaaaa 
      tttgcaaag cacaaccgt tccagatca cgtcgtgac 
      agaatcgat taccacggt ctcaactcc gagtgaagt 
      gacgacaaa attagctga gaaagaact cgtggtgga 
      caagaacgt gtcgccccc cagacatgt aaacacagt 
      catccacaa cagccccat aaaatacat agagaaaga 
      tgcgaagca ctccgagga gtctttagc gacattcgc 
      tcccgaaat aaagaatga tcaatacgt gctgttaga 
      taaggagga gctctgggg gatgctaaa ggaggttgt 
      tgatgacca acttaaaaa gaaaacgag ttcgaagat 
      agtcgacgg cgagaagct ccgcagcca aggcttcac 
      gtaattatt tgtcccaca tttttgaac aagaatacg 
      tgttgtgat tagaataac tgatctcgt ttacgattg 
      taaattgta catataatt agtgctggg tttattgat 
      cgggatgcg gattctgct tggaaattt atgtagatt 
      aaaccacaa ttagcaata tttgtaagt gtcttttgg 
      tatcaaaat caattacaa tcaacatga ttggccgca 
      atgtggtgc ggtggaagt gggtaattt gtcagcacg 
      gatttatat caacacggg ctggtgcca aaatataat 
      tagctcgaa ctgtacata cagataagt caattgcat 
      cacagtact cactaattc gggaaaaag gatcgtttc 
      aaacactga taaatgtat taaaagtgt acataagaa 
      aagtgtaaa ttatccgcc cattaatta taaattaaa 
      cttgctgaa atggtaatc gtaagaatt tcatgtctg 
      ctgtatttt aggcgtaag aactcttta ctgtacata 
      aggcaagtg tgacgcatt tttgtaaat gtgccaaat 
      cgttattcc agttattcc accgaatca acttgattt 
      ttttactcg tgtacctgt attatttca tactctttg 
      ttgccaaaa acgaggtag ttctagttg tacccagaa 
      aacatcgaa atttttata accgatttt cgacttgta 
      cataacagt gcattcagt cttttccca tagttttga 
      taagctata ttgcacaga attttgtaa ctctaccta 
      caggctgtc acagttatt tgctggatt tatacataa 
      cataacatg tacactgct caccaatgt tagatatag 
      gaggaaaat aatgttatt ttctcagta atgcttcag 
      ccaaagtta tataatgca ttttagctc tattttaaa 
      tacaaaatt gtacgtatt catgtcata tcaatgtgt 
      attatttac cagttcaat aaattatga ttttttaa