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Gene information
General information
TC034549 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 5841599..5844106
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034549
    iBeetle screen iB_02644

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034549 RA
      MELPRKKNG LLNQQKKVR LDMQGEILQ HAADIPFDL 
      KFISYPFDS SRFIQFNPT SLERNYRYE VLTEHDLGV 
      AIDLINKDI YAVEPGAML DPADEKLLE EDILTPQDS 
      KRSRHHARS VSWLRRTEY ISTEQTRFQ PQSMDKVEA 
      KVGYSIKKN LKNETLYMD RDSQIKAIE KTFSDTTNP 
      IEKHYSKPN VTAVEVLPV FPDFNLWKY PCAQVIFDS 
      DPAPVGKQV PAQIEEMSQ AMIRGVMDE RGEQFVAYF 
      LPTEETLVK RREDFANEI PYQDDEEYE YKMAREYNW 
      NVKSKASKG YEENYFFVI RQDGAYYNE LETRVRLSK 
      RRQKVGQPA SNTRLIVKH RPMDANEFR MQRYREKQL 
      EPAGDEDEE MEMEQNEEP QENEKESDA ESSRSKSPS 
      RSRSPSRSR SQSKSPSRS RSRSKSRSR SRSKSRSRS 
      RSKSASRSR SRSRSASGS RSPSKSPSR SRSGSKSRS 
      PSRSKSRSK SPSRSRSGS RSRSGSRSG SESGSKSGQ
    CDS sequence
    • >TC034549 RA
      atggaactt ccacgcaag aaaaacggc ctgttaaac 
      cagcagaaa aaagtccga cttgatatg cagggtgaa 
      atattgcaa cacgctgct gatatcccg tttgatttg 
      aaatttatc tcatacccg tttgattca tcaaggttt 
      atacaattt aatccaacc tcgttggag agaaattat 
      cggtatgaa gtgctcact gaacatgat ttgggcgtt 
      gccattgac ttgattaac aaagatatt tacgctgtg 
      gaaccaggt gctatgctt gatccagct gatgagaaa 
      ctcctagaa gaagatatc ctaacacct caagactcg 
      aaaaggtcc agacaccat gcaagatct gtctcttgg 
      cttcgcagg actgaatac atttcaaca gaacagact 
      cgattccag ccacaaagt atggataaa gtcgaagcg 
      aaagttggc tacagtatt aagaagaat ttgaagaat 
      gaaacattg tacatggac cgtgattca caaatcaag 
      gctattgag aaaacgttc agtgataca acgaatccg 
      attgaaaag cattacagc aagccgaat gtgactgct 
      gttgaagta ttgccagtg tttcccgat ttcaatctg 
      tggaaatat ccatgtgct caggttatt tttgattca 
      gatccagcg cctgttggt aaacaagtg cccgcgcag 
      attgaggaa atgtcacaa gctatgatc agaggtgtg 
      atggacgaa cgtggcgaa caattcgtc gcctacttc 
      ctcccaact gaagagact ctagttaaa agacgggaa 
      gactttgct aacgagata ccataccaa gacgatgaa 
      gaatatgag tataaaatg gcaagagaa tacaactgg 
      aacgtgaag agtaaagca agcaaaggt tacgaagaa 
      aattacttc ttcgtaatc agacaagat ggtgcctac 
      tataatgag ctagaaacc cgtgtcaga ttgagtaaa 
      cgtcgccag aaagttggc caacctgcc agcaacacc 
      cgtcttatc gtcaaacac cgaccaatg gatgcgaac 
      gaattccga atgcaaaga taccgcgaa aaacaactc 
      gaacctgca ggcgatgaa gacgaagaa atggaaatg 
      gaacaaaac gaagaacca caagaaaac gaaaaagaa 
      agcgacgcg gaatcgtca agatccaaa tcaccgtca 
      cgttcaaga tctccgtct agatcccga tcacagtct 
      aaatctccc tcacggtcc agatccaga tccaaatcg 
      cgatcaaga tccagatcc aaatcgcgg tcaagatcc 
      agatcaaaa tctgcgtct agatccaga tcacgttca 
      cgctcggct tccggatcg cgatctcct tccaaatcg 
      ccgtcacga tccagatcc ggatccaaa tcacgatca 
      ccatcacgt tccaaatca cggagtaaa tcaccgtcc 
      agatccaga tccggatcg cggtcaaga tccggatcc 
      cgatctgga tccgaatcc ggcagcaag tctggacag 
      tga
    mRNA sequence
    • >TC034549 RA
      tatgttttg acaactgtg cgtgtataa gttgtaaat 
      ttattgaaa tagcttgaa aatattttt attaagtta 
      gaaaatttg gtgtatatt ttacaatgc ctccaacga 
      tacaaaatg gaacttcca cgcaagaaa aacggcctg 
      ttaaaccag cagaaaaaa gtccgactt gatatgcag 
      ggtgaaata ttgcaacac gctgctgat atcccgttt 
      gatttgaaa tttatctca tacccgttt gattcatca 
      aggtttata caatttaat ccaacctcg ttggagaga 
      aattatcgg tatgaagtg ctcactgaa catgatttg 
      ggcgttgcc attgacttg attaacaaa gatatttac 
      gctgtggaa ccaggtgct atgcttgat ccagctgat 
      gagaaactc ctagaagaa gatatccta acacctcaa 
      gactcgaaa aggtccaga caccatgca agatctgtc 
      tcttggctt cgcaggact gaatacatt tcaacagaa 
      cagactcga ttccagcca caaagtatg gataaagtc 
      gaagcgaaa gttggctac agtattaag aagaatttg 
      aagaatgaa acattgtac atggaccgt gattcacaa 
      atcaaggct attgagaaa acgttcagt gatacaacg 
      aatccgatt gaaaagcat tacagcaag ccgaatgtg 
      actgctgtt gaagtattg ccagtgttt cccgatttc 
      aatctgtgg aaatatcca tgtgctcag gttattttt 
      gattcagat ccagcgcct gttggtaaa caagtgccc 
      gcgcagatt gaggaaatg tcacaagct atgatcaga 
      ggtgtgatg gacgaacgt ggcgaacaa ttcgtcgcc 
      tacttcctc ccaactgaa gagactcta gttaaaaga 
      cgggaagac tttgctaac gagatacca taccaagac 
      gatgaagaa tatgagtat aaaatggca agagaatac 
      aactggaac gtgaagagt aaagcaagc aaaggttac 
      gaagaaaat tacttcttc gtaatcaga caagatggt 
      gcctactat aatgagcta gaaacccgt gtcagattg 
      agtaaacgt cgccagaaa gttggccaa cctgccagc 
      aacacccgt cttatcgtc aaacaccga ccaatggat 
      gcgaacgaa ttccgaatg caaagatac cgcgaaaaa 
      caactcgaa cctgcaggc gatgaagac gaagaaatg 
      gaaatggaa caaaacgaa gaaccacaa gaaaacgaa 
      aaagaaagc gacgcggaa tcgtcaaga tccaaatca 
      ccgtcacgt tcaagatct ccgtctaga tcccgatca 
      cagtctaaa tctccctca cggtccaga tccagatcc 
      aaatcgcga tcaagatcc agatccaaa tcgcggtca 
      agatccaga tcaaaatct gcgtctaga tccagatca 
      cgttcacgc tcggcttcc ggatcgcga tctccttcc 
      aaatcgccg tcacgatcc agatccgga tccaaatca 
      cgatcacca tcacgttcc aaatcacgg agtaaatca 
      ccgtccaga tccagatcc ggatcgcgg tcaagatcc 
      ggatcccga tctggatcc gaatccggc agcaagtct 
      ggacagtga ttgaaccac taacgtttt atttaagtg 
      tcaagtcat tgtttattg gatttagat gtaaatatg 
      gtttaataa acaatacct gaatat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY