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Gene information
General information
TC000797 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 290376..323174
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000797
    iBeetle screen iB_02643
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): less than 30%
    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva random constricted - Penetrance (percentage of animals/eggs on slide): less than 30%

    head decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head appendages random partiallynot present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Comment: Procephalic head is often not present.
    legs partially sometimesnot present - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Pupal morphology
    legs slightly orientation irregular - Number of animals showing the phenotype: 2

    legs slightly shortened - Number of animals showing the phenotype: 2
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000797 RA
      MLHYASRYG HKDIVKYLL QHAPPSILN 
      IKDVNLGQT ALHKAAAYK WRSICCMLV 
      AGGAALDIT DRRGLTPRL LALQSDDHD 
      LAAYLESQE HFQLVSSSL GTVV
    • >TC000797 RB
      MQRLRTTFK RSRTPTGAE MKTQSSLEV PKQVRSASF 
      DEIQLEAKR LQSGGQSTD PSALLRVPQ FPGQRSRSV 
      DSGGSEESG NFLEVPARR FQRRRSSSS KSPVCVHCV 
      CLEEYAKLH PAEEQPPEP PDELPLPTS SISDSSSEC 
      EEEVLPECS ITVTLSPIL PPPPIQSPP PLPASPVIE 
      FFPTEDVQT PTTPTRRKS IHRQEAFFA EPTGTSLEN 
      MSDGPSELY SEGTSEGAL SEGDAVNAT IPGLVVRDI 
      YLQVPDLKR DRAASVDSC FSKVSQGKT EELQHSGVS 
      LEVPVGPNV ALRSRSVDI VLPTDEHAR YKALAMAAA 
      PGTSSSYYK GKVVGDVGE RVLRSTPDW GETAINGDH 
      LWVPTSVSG DFCYIGDMD CTKHGARMK CSACNVVAH 
      QNCIGILMD KMRFTCKPT FRDVGVRQY REQTSIHHH 
      WVHRRSEKG KCRQCGKSF QSKLSFSSK EIVAISCSW 
      CKAAYHNKD TCFNMQRIG EECNLGVHR SIVVPPSWI 
      VKLPRKGSF KSSLRKSPK KRASSKRKT KEKDKEHRT 
      FAIKPIPTT NVTPVIVFI NPKSGGNQG SKLLQKFQW 
      LLNPRQVFD LTQGGPKMG LELFKKVPN LRVLACGGD 
      GTVGWVLSV IDQIGISPA PAVGVLPLG TGNDLARAL 
      GWGGGYTDE PISKILSNI SASETVLLD RWSLEVEKN 
      PNAEANEGG KDNLPLNVV NNYYSLGVD AHIALEFHE 
      AREAHPEKF NSRLRNKMF YGQMGGKDL LKRKWKGLA 
      DFVTLECDG KDLTPKLKE LKVHAIVFL NIPSYGGGT 
      RPWNRSMGT CEPSTDDGL IEVVGLTTY QLPLLQAGG 
      HGTCITQCR SAKIVTSKT IPMQVDGEA CKLNPSIIN 
      LSLLNKAPM LAKRKCGKP NVPQTALDN LKICVHRIS 
      MADYEQHHY DKDLLAQAA ISVGTIEVN PIWDLEQVR 
      ALINKFIED CKDQQKIHP EWCFLDSCT AERFFRIDK 
      AQENLHYVL DIAVDNLYI LECGGGTVP QTPEDETAT 
      PSPSSINQT SYIIPEMRV SDEGSLDSP SDQPPMSPK 
      ILQSPEEFP SETIAEENR NGDKQLARI SPDASVKIR 
      RDLPQKPNE ALLKAAKVG DLKTVKELH SQGYSLLTK 
      DPAGQTMLH YASRYGHKD IVKYLLQHA PPSILNIKD 
      VNLGQTALH KAAAYKWRS ICCMLVAGG AALDITDRR 
      GLTPRLLAL QSDDHDLAA YLESQEHFQ LVSSSLGTV 
      V
    • >TC000797 RC
      MQRLRTTFK RSRTPTGAE MKTQSSLEV PKQVRSASF 
      DEIQLEAKR LQSGGQSTD PSALLRVPQ FPGQRSRSV 
      DSGGSEESG NFLEVPARR FQRRRSSSS KSPVCVHCV 
      CLEEYAKLH PAEEQPPEP PDELPLPTS SISDSSSEC 
      EEEVLPECS ITVTLSPIL PPPPIQSPP PLPASPVIE 
      FFPTEDVQT PTTPTRRKS IHRQEAFFA EPTGTSLEN 
      MSDGPSELY SEGTSEGAL SEGDAVNAT IPGLVVRDI 
      YLQVPDLKR DRAASVDSC FSKVSQGKT EELQHSGVS 
      LEVPVGPNV ALRSRSVDI VLPTDEHAR YKALAMAAA 
      PGTSSSYYK GKVVGDVGE RVLRSTPDW GETAINGDH 
      LWVPTSVSG DFCYIGDMD CTKHGARMK CSACNVVAH 
      QNCIGILMD KMRFTCKPT FRDVGVRQY REQTSIHHH 
      WVHRRSEKG KCRQCGKSF QSKLSFSSK EIVAISCSW 
      CKAAYHNKD TCFNMQRIG EECNLGVHR SIVVPPSWI 
      VKLPRKGSF KSSLRKSPK KRASSKRKT KEKDKEHRT 
      FAIKPIPTT NVTPVIVFI NPKSGGNQG SKLLQKFQW 
      LLNPRQVFD LTQGGPKMG LELFKKVPN LRVLACGGD 
      GTVGWVLSV IDQIGISPA PAVGVLPLG TGNDLARAL 
      GWGGGYTDE PISKILSNI SASETVLLD RWSLEVEKN 
      PNAEANEGG KDNLPLNVV NNYYSLGVD AHIALEFHE 
      AREAHPEKF NSRLRNKMF YGQMGGKDL LKRKWKGLA 
      DFVTLECDG KDLTPKLKE LKVHAIVFL NIPSYGGGT 
      RPWNRSMGT CEPSTDDGL IEVVGLTTY QLPLLQAGG 
      HGTCITQCR SAKIVTSKT IPMQVDGEA CKLNPSIIN 
      LSLLNKAPM LAKRKCGKP NVPQTALDN LKICVHRIS 
      MADYEQHHY DKDLLAQAA ISVGTIEVN PIWDLEQVR 
      ALINKFIED CKDQQKIHP EWCFLDSCT AERFFRIDK 
      AQENLHYVL DIAVDNLYI LECGGGTVP QTPEDETAT 
      PSPSSINQT SYIIPEMRV SDEGSLDSP SDQPPMSPK 
      ILQSPEEFP SETIAEENR NGDKQLARI SPDASVKIR 
      RYLNFILAL TYFYTKPLI EIE
    CDS sequence
    • >TC000797 RA
      atgttgcat tacgcctca cggtatggc cacaaagat 
      attgttaaa tacttactc cagcacgcc ccaccttca 
      attctgaat atcaaagac gtgaactta ggacaaacg 
      gcgcttcat aaagcagca gcgtacaag tggcgcagt 
      atttgttgt atgttagtc gccggaggc gcagctttg 
      gatattaca gatcgtcga ggtttaact ccgagatta 
      ttagcgtta caatccgac gatcacgat ttggctgca 
      tacctcgaa agtcaggaa cactttcag ttggtgtcc 
      tccagttta gggactgtt gtatga
    • >TC000797 RB
      atgcagcgg ctcaggacc acgtttaaa cggtcccga 
      actccgact ggtgccgaa atgaagact caaagcagt 
      ctggaggtg cccaagcag gtgcgctct gcctctttc 
      gacgagatt cagttggag gcgaagcga ctgcaatcg 
      gggggtcag tcgaccgac ccttcggcg cttctcaga 
      gtgccccag ttccccgga cagcgttcg aggagtgtc 
      gattcggga ggtagcgaa gaatccggc aatttcctc 
      gaagtaccg gcgcgacga tttcaacgg cgccgctcc 
      tcaagcagc aaaagtccg gtctgtgtg cactgcgtt 
      tgcttggag gagtacgcc aaactacat ccggcggaa 
      gaacagccg ccggaaccg ccggacgag ttgccgcta 
      cccacttcc agcatcagc gactcctcc agcgaatgc 
      gaagaagaa gtcctccca gaatgctcc atcacagtc 
      actctctcc cccatacta cctccccct cccatccaa 
      tccccacca ccgcttcct gcatcccct gtcatcgaa 
      tttttccca actgaagat gtccaaaca ccgaccacg 
      ccaacaagg cggaaatca attcaccgt caagaagct 
      tttttcgcc gaaccaact ggaacctct ttggagaat 
      atgtcagat ggtccgtct gaactgtac tcagagggg 
      acatcggaa ggagcactg tctgaaggg gacgctgtt 
      aacgccacc ataccgggc ttggtagtc cgagatatt 
      tacttgcaa gtccctgac ttgaaacgc gacagagct 
      gcgtctgtt gattcgtgt ttttcgaaa gtctcgcaa 
      ggaaaaacc gaggagtta caacattcc ggggttagt 
      ttggaggtg ccagtgggg ccaaatgtg gctttacgg 
      tcacggtct gtggatatc gtattgcca acagatgaa 
      catgccagg tacaaggcc ttggctatg gccgcagcg 
      cctggcact tcttcgtct tattacaag gggaaagtt 
      gtcggcgat gttggagaa agggtcctc cggagtact 
      ccggactgg ggcgaaact gcaatcaac ggtgatcat 
      ttatgggtt cccacatcg gtctcgggt gacttttgc 
      tacatcgga gatatggac tgcacgaaa cacggtgcc 
      cggatgaag tgttccgct tgtaatgtg gtcgcgcac 
      cagaattgc atcgggatc ttgatggat aagatgcga 
      tttacatgc aagcctacg ttccgtgat gtgggagtt 
      agacagtac agagaacag acctccatc catcaccat 
      tgggtccac cgacggtca gagaagggt aaatgtcga 
      caatgcggc aagtcattc cagtccaag ttatcgttt 
      agttcgaaa gaaatagtc gcaattagt tgttcgtgg 
      tgcaaggct gcttatcac aataaggac acctgcttt 
      aatatgcaa cgaataggc gaggagtgc aatttaggt 
      gttcatcgc agcattgtg gttcctcct tcgtggatc 
      gtgaaactg cctcgcaaa ggaagtttc aagtcaagt 
      ctgagaaaa tctccgaag aaacgggct tccagcaag 
      cgaaaaacc aaggaaaag gataaagag cacagaacg 
      ttcgccatc aagccgatc cccacaacc aacgtaacg 
      cccgtcata gtgttcatc aaccctaag tccgggggt 
      aaccaagga tcaaagttg ctccagaaa ttccagtgg 
      ttgttgaat ccgcgacag gtgtttgac ttgacacag 
      ggcggtccc aagatgggt ctcgaactg ttcaagaaa 
      gtacctaat ctacgtgtt cttgcctgc ggcggagat 
      ggaaccgtt ggttgggtt ttaagtgtt atcgaccaa 
      atcgggatc tctcctgct ccagcggtc ggcgttctt 
      cctctagga acaggaaat gatctggca agggcgctc 
      ggatggggt gggggttat acggatgaa ccaatatcg 
      aaaatcctg tccaatatt agcgctagc gagactgtg 
      ttattagat aggtggtct ttagaggtt gagaagaac 
      ccgaacgct gaggctaac gaaggcggc aaggataat 
      ttgcctctc aacgttgta aataattat tattcatta 
      ggtgtcgat gctcatatt gccttagaa ttccacgaa 
      gctcgagaa gctcatccc gaaaaattc aattctaga 
      ttaaggaac aaaatgttt tatggccag atgggcggc 
      aaagacttg ctcaagcga aagtggaag ggtttagct 
      gatttcgtc acgttagaa tgcgacggc aaagaccta 
      acacccaaa ctgaaagag cttaaagta cacgcgatt 
      gtcttctta aacattcca agttatggt gggggaaca 
      aggccctgg aaccgatcc atgggcacg tgtgagcca 
      agcactgac gacggtttg attgaagtc gtcggtttg 
      acaacatat cagcttcct ttgctacaa gctggaggc 
      cacggtact tgcattacg caatgtagg tcggcaaaa 
      atcgtaaca tccaaaaca atacccatg caagtggat 
      ggggaagct tgcaaatta aacccttcg atcataaat 
      ctttcactg ttaaataaa gctccaatg ctggcaaag 
      cggaagtgt ggcaaaccc aacgtaccg caaactgct 
      ctcgacaat ctgaaaatt tgtgttcat agaattagc 
      atggctgac tatgaacag caccactat gacaaggat 
      ctattagct caagcggca ataagcgtt ggtacaatc 
      gaagttaac ccaatctgg gatttggaa caagtgaga 
      gcgttaatt aacaaattt atagaagac tgcaaagac 
      cagcaaaaa atccacccc gagtggtgt tttttagac 
      tcatgtaca gctgaacgg ttcttccgc atcgacaag 
      gcccaagaa aaccttcat tacgtgctg gatatcgcg 
      gtcgataac ttatacatt ttggaatgc ggaggtgga 
      actgttcct caaactccc gaagacgaa acagccaca 
      ccgtctccc agttctatt aatcaaaca tcgtacatt 
      attccagaa atgcgcgtt agtgatgaa ggcagcctg 
      gattcgccg agcgatcaa ccaccgatg agtccaaaa 
      atcctgcaa tctccggaa gaatttccc agcgagaca 
      attgcggaa gagaaccgc aacggcgac aagcaatta 
      gcgcgcatt tcccccgac gctagcgtg aaaataaga 
      agggacttg ccacaaaag ccgaacgaa gctctctta 
      aaagccgcc aaagtgggg gacttgaaa acagtcaag 
      gaattacac agtcagggc tactctcta cttaccaaa 
      gacccagcc ggccaaaca atgttgcat tacgcctca 
      cggtatggc cacaaagat attgttaaa tacttactc 
      cagcacgcc ccaccttca attctgaat atcaaagac 
      gtgaactta ggacaaacg gcgcttcat aaagcagca 
      gcgtacaag tggcgcagt atttgttgt atgttagtc 
      gccggaggc gcagctttg gatattaca gatcgtcga 
      ggtttaact ccgagatta ttagcgtta caatccgac 
      gatcacgat ttggctgca tacctcgaa agtcaggaa 
      cactttcag ttggtgtcc tccagttta gggactgtt 
      gtatga
    • >TC000797 RC
      atgcagcgg ctcaggacc acgtttaaa cggtcccga 
      actccgact ggtgccgaa atgaagact caaagcagt 
      ctggaggtg cccaagcag gtgcgctct gcctctttc 
      gacgagatt cagttggag gcgaagcga ctgcaatcg 
      gggggtcag tcgaccgac ccttcggcg cttctcaga 
      gtgccccag ttccccgga cagcgttcg aggagtgtc 
      gattcggga ggtagcgaa gaatccggc aatttcctc 
      gaagtaccg gcgcgacga tttcaacgg cgccgctcc 
      tcaagcagc aaaagtccg gtctgtgtg cactgcgtt 
      tgcttggag gagtacgcc aaactacat ccggcggaa 
      gaacagccg ccggaaccg ccggacgag ttgccgcta 
      cccacttcc agcatcagc gactcctcc agcgaatgc 
      gaagaagaa gtcctccca gaatgctcc atcacagtc 
      actctctcc cccatacta cctccccct cccatccaa 
      tccccacca ccgcttcct gcatcccct gtcatcgaa 
      tttttccca actgaagat gtccaaaca ccgaccacg 
      ccaacaagg cggaaatca attcaccgt caagaagct 
      tttttcgcc gaaccaact ggaacctct ttggagaat 
      atgtcagat ggtccgtct gaactgtac tcagagggg 
      acatcggaa ggagcactg tctgaaggg gacgctgtt 
      aacgccacc ataccgggc ttggtagtc cgagatatt 
      tacttgcaa gtccctgac ttgaaacgc gacagagct 
      gcgtctgtt gattcgtgt ttttcgaaa gtctcgcaa 
      ggaaaaacc gaggagtta caacattcc ggggttagt 
      ttggaggtg ccagtgggg ccaaatgtg gctttacgg 
      tcacggtct gtggatatc gtattgcca acagatgaa 
      catgccagg tacaaggcc ttggctatg gccgcagcg 
      cctggcact tcttcgtct tattacaag gggaaagtt 
      gtcggcgat gttggagaa agggtcctc cggagtact 
      ccggactgg ggcgaaact gcaatcaac ggtgatcat 
      ttatgggtt cccacatcg gtctcgggt gacttttgc 
      tacatcgga gatatggac tgcacgaaa cacggtgcc 
      cggatgaag tgttccgct tgtaatgtg gtcgcgcac 
      cagaattgc atcgggatc ttgatggat aagatgcga 
      tttacatgc aagcctacg ttccgtgat gtgggagtt 
      agacagtac agagaacag acctccatc catcaccat 
      tgggtccac cgacggtca gagaagggt aaatgtcga 
      caatgcggc aagtcattc cagtccaag ttatcgttt 
      agttcgaaa gaaatagtc gcaattagt tgttcgtgg 
      tgcaaggct gcttatcac aataaggac acctgcttt 
      aatatgcaa cgaataggc gaggagtgc aatttaggt 
      gttcatcgc agcattgtg gttcctcct tcgtggatc 
      gtgaaactg cctcgcaaa ggaagtttc aagtcaagt 
      ctgagaaaa tctccgaag aaacgggct tccagcaag 
      cgaaaaacc aaggaaaag gataaagag cacagaacg 
      ttcgccatc aagccgatc cccacaacc aacgtaacg 
      cccgtcata gtgttcatc aaccctaag tccgggggt 
      aaccaagga tcaaagttg ctccagaaa ttccagtgg 
      ttgttgaat ccgcgacag gtgtttgac ttgacacag 
      ggcggtccc aagatgggt ctcgaactg ttcaagaaa 
      gtacctaat ctacgtgtt cttgcctgc ggcggagat 
      ggaaccgtt ggttgggtt ttaagtgtt atcgaccaa 
      atcgggatc tctcctgct ccagcggtc ggcgttctt 
      cctctagga acaggaaat gatctggca agggcgctc 
      ggatggggt gggggttat acggatgaa ccaatatcg 
      aaaatcctg tccaatatt agcgctagc gagactgtg 
      ttattagat aggtggtct ttagaggtt gagaagaac 
      ccgaacgct gaggctaac gaaggcggc aaggataat 
      ttgcctctc aacgttgta aataattat tattcatta 
      ggtgtcgat gctcatatt gccttagaa ttccacgaa 
      gctcgagaa gctcatccc gaaaaattc aattctaga 
      ttaaggaac aaaatgttt tatggccag atgggcggc 
      aaagacttg ctcaagcga aagtggaag ggtttagct 
      gatttcgtc acgttagaa tgcgacggc aaagaccta 
      acacccaaa ctgaaagag cttaaagta cacgcgatt 
      gtcttctta aacattcca agttatggt gggggaaca 
      aggccctgg aaccgatcc atgggcacg tgtgagcca 
      agcactgac gacggtttg attgaagtc gtcggtttg 
      acaacatat cagcttcct ttgctacaa gctggaggc 
      cacggtact tgcattacg caatgtagg tcggcaaaa 
      atcgtaaca tccaaaaca atacccatg caagtggat 
      ggggaagct tgcaaatta aacccttcg atcataaat 
      ctttcactg ttaaataaa gctccaatg ctggcaaag 
      cggaagtgt ggcaaaccc aacgtaccg caaactgct 
      ctcgacaat ctgaaaatt tgtgttcat agaattagc 
      atggctgac tatgaacag caccactat gacaaggat 
      ctattagct caagcggca ataagcgtt ggtacaatc 
      gaagttaac ccaatctgg gatttggaa caagtgaga 
      gcgttaatt aacaaattt atagaagac tgcaaagac 
      cagcaaaaa atccacccc gagtggtgt tttttagac 
      tcatgtaca gctgaacgg ttcttccgc atcgacaag 
      gcccaagaa aaccttcat tacgtgctg gatatcgcg 
      gtcgataac ttatacatt ttggaatgc ggaggtgga 
      actgttcct caaactccc gaagacgaa acagccaca 
      ccgtctccc agttctatt aatcaaaca tcgtacatt 
      attccagaa atgcgcgtt agtgatgaa ggcagcctg 
      gattcgccg agcgatcaa ccaccgatg agtccaaaa 
      atcctgcaa tctccggaa gaatttccc agcgagaca 
      attgcggaa gagaaccgc aacggcgac aagcaatta 
      gcgcgcatt tcccccgac gctagcgtg aaaataaga 
      aggtactta aatttcatt ttagctttg acttacttt 
      tataccaag cctctgatt gaaatcgaa taa
    mRNA sequence
    • >TC000797 RA
      agggacttg ccacaaaag ccgaacgaa gctctctta 
      aaagccgcc aaagtgggg gacttgaaa acagtcaag 
      gaattacac agtcagggc tactctcta cttaccaaa 
      gacccagcc ggccaaaca atgttgcat tacgcctca 
      cggtatggc cacaaagat attgttaaa tacttactc 
      cagcacgcc ccaccttca attctgaat atcaaagac 
      gtgaactta ggacaaacg gcgcttcat aaagcagca 
      gcgtacaag tggcgcagt atttgttgt atgttagtc 
      gccggaggc gcagctttg gatattaca gatcgtcga 
      ggtttaact ccgagatta ttagcgtta caatccgac 
      gatcacgat ttggctgca tacctcgaa agtcaggaa 
      cactttcag ttggtgtcc tccagttta gggactgtt 
      gtatgaaac gagcaataa ttgtattaa attttcctg 
      tcactggtt tctctaaaa ttgccttac tacttgtat 
      tcaaaattg ctaatgtat gattgtacc taaaatact 
      acaaatgtg ataatacta aagttaagc ttttttatt 
      attttttta tttatttgt ttcattatt gtttgtttg 
      ataaagtta gttaaagtg ttatatttt ttcactaga 
      acgtattct tccaggagg agaacaatc ataaagtat 
      tgtaaataa gcgataagt gtttaaagt tattgacaa 
      acatatcac tcatgacat tcctgtaaa gtttgtaga 
      cgtaagtgt gttgaataa tttgttacg ttttgatct 
      gttgaatca tttgcttga ggaaattcg aacacaggg 
      caataaatt agattcgat gtgtctagc atattatcc 
      ataaatgtt atactcttt tattaaatt gttacgttt 
      tacgtgtac ataagtact gcaacgaat ttcaatatt 
      tcccgaaaa gacaatctg taaatgtga aataaatag 
      ttgttgtaa gtgctgtca tgtattaat agttgtata 
      catttttgt ctctttgaa agttttatt attcgattt 
      ttgaactgc attaagcat ctaggattt gaaatatta 
      acagtaata gtgtgtaaa tatgtagcc attaggcat 
      cactctgta gataatcta gtcttatag tgatacaac 
      tgcagaaat ttatataaa gtgcgttaa caacttttt 
      acaaatttc tgcaattac gatgagtaa atcaatgcc 
      ttacgaact ttgagtttt ttaacagaa tagtcaata 
      cgtagctag tttcgtcac tagaatact ctatttata 
      tttttagtc tattttatc ttttatcaa tattatctc 
      ctagaatct ttgcttttt ttaatttta tcaattcac 
      gttgttggt agattttac ttaacatcg gttccattc 
      aagatggaa taatctcgc tcgatattc tttcactag 
      ttactatga aaatgtgat tgaaactaa atgtacata 
      aatatttat gtaattact tcttgtaaa tttaatcta 
      gtctttaca ggtgtaagt tgtagcagt agttgaggg 
      aacattctt acaatgata gtagatata aaccacatt 
      tttgccatt taaacacca ctcagtcag ttcagttga 
      ttgcagttt attggaaat aaaattacc tgcatacgt 
      acggtttta tttacctta cgttacgtg aacaacttg 
      taaatacac ctgaacatt gtgactagt attaaatta 
      ggtagagtt taatgactt gttatctag ttatttcat 
      attaatctg acaccaaaa acgaagtat aaaattatt 
      gattaacaa cgtatttgc acgtaccca gagttttaa 
      acctaggaa taattatgc aacgaatct ggcccagac 
      aatactcac tttgggcca gtttcgtct ttatattac 
      gctgcctcg gaactaatt tcgtgttaa tgcgatatt 
      atagggttt ttataatca atgtcgagg aaattagcc 
      acattcacg ctctatatt atcaattta accacgaat 
      tttttggtc aattatttg gttgagtga ggatttttt 
      taacctgtc catttaggt aactaaggg tccaagcat 
      gtgtctagg tctatttta aaaaattcc attttatgt 
      ggtaaaacg tattgggcc agttgtaaa tttgcaaag 
      ttttctcca acctcatac cgagcactg tttgtagca 
      cagtttgca aagggtttc tatagcacc atgaccacg 
      ccatatttt tgtaattac acaagcaaa gatgtttta 
      ataatattg taaagtctc ttattgtaa atcgtgtaa 
      aatggctga gatttaact tcgatttga tcattttct 
      atgcctaac tatgtatta taatattat caaatataa 
      atatattta agaa
    • >TC000797 RB
      tagaaccgc gataaaggg gcaaaacag ttacagaat 
      gggcgtaga gtgggacaa gggcggcag cagcccccg 
      ctgagtcat gcagcggct caggaccac gtttaaacg 
      gtcccgaac tccgactgg tgccgaaat gaagactca 
      aagcagtct ggaggtgcc caagcaggt gcgctctgc 
      ctctttcga cgagattca gttggaggc gaagcgact 
      gcaatcggg gggtcagtc gaccgaccc ttcggcgct 
      tctcagagt gccccagtt ccccggaca gcgttcgag 
      gagtgtcga ttcgggagg tagcgaaga atccggcaa 
      tttcctcga agtaccggc gcgacgatt tcaacggcg 
      ccgctcctc aagcagcaa aagtccggt ctgtgtgca 
      ctgcgtttg cttggagga gtacgccaa actacatcc 
      ggcggaaga acagccgcc ggaaccgcc ggacgagtt 
      gccgctacc cacttccag catcagcga ctcctccag 
      cgaatgcga agaagaagt cctcccaga atgctccat 
      cacagtcac tctctcccc catactacc tccccctcc 
      catccaatc cccaccacc gcttcctgc atcccctgt 
      catcgaatt tttcccaac tgaagatgt ccaaacacc 
      gaccacgcc aacaaggcg gaaatcaat tcaccgtca 
      agaagcttt tttcgccga accaactgg aacctcttt 
      ggagaatat gtcagatgg tccgtctga actgtactc 
      agaggggac atcggaagg agcactgtc tgaagggga 
      cgctgttaa cgccaccat accgggctt ggtagtccg 
      agatattta cttgcaagt ccctgactt gaaacgcga 
      cagagctgc gtctgttga ttcgtgttt ttcgaaagt 
      ctcgcaagg aaaaaccga ggagttaca acattccgg 
      ggttagttt ggaggtgcc agtggggcc aaatgtggc 
      tttacggtc acggtctgt ggatatcgt attgccaac 
      agatgaaca tgccaggta caaggcctt ggctatggc 
      cgcagcgcc tggcacttc ttcgtctta ttacaaggg 
      gaaagttgt cggcgatgt tggagaaag ggtcctccg 
      gagtactcc ggactgggg cgaaactgc aatcaacgg 
      tgatcattt atgggttcc cacatcggt ctcgggtga 
      cttttgcta catcggaga tatggactg cacgaaaca 
      cggtgcccg gatgaagtg ttccgcttg taatgtggt 
      cgcgcacca gaattgcat cgggatctt gatggataa 
      gatgcgatt tacatgcaa gcctacgtt ccgtgatgt 
      gggagttag acagtacag agaacagac ctccatcca 
      tcaccattg ggtccaccg acggtcaga gaagggtaa 
      atgtcgaca atgcggcaa gtcattcca gtccaagtt 
      atcgtttag ttcgaaaga aatagtcgc aattagttg 
      ttcgtggtg caaggctgc ttatcacaa taaggacac 
      ctgctttaa tatgcaacg aataggcga ggagtgcaa 
      tttaggtgt tcatcgcag cattgtggt tcctccttc 
      gtggatcgt gaaactgcc tcgcaaagg aagtttcaa 
      gtcaagtct gagaaaatc tccgaagaa acgggcttc 
      cagcaagcg aaaaaccaa ggaaaagga taaagagca 
      cagaacgtt cgccatcaa gccgatccc cacaaccaa 
      cgtaacgcc cgtcatagt gttcatcaa ccctaagtc 
      cgggggtaa ccaaggatc aaagttgct ccagaaatt 
      ccagtggtt gttgaatcc gcgacaggt gtttgactt 
      gacacaggg cggtcccaa gatgggtct cgaactgtt 
      caagaaagt acctaatct acgtgttct tgcctgcgg 
      cggagatgg aaccgttgg ttgggtttt aagtgttat 
      cgaccaaat cgggatctc tcctgctcc agcggtcgg 
      cgttcttcc tctaggaac aggaaatga tctggcaag 
      ggcgctcgg atggggtgg gggttatac ggatgaacc 
      aatatcgaa aatcctgtc caatattag cgctagcga 
      gactgtgtt attagatag gtggtcttt agaggttga 
      gaagaaccc gaacgctga ggctaacga aggcggcaa 
      ggataattt gcctctcaa cgttgtaaa taattatta 
      ttcattagg tgtcgatgc tcatattgc cttagaatt 
      ccacgaagc tcgagaagc tcatcccga aaaattcaa 
      ttctagatt aaggaacaa aatgtttta tggccagat 
      gggcggcaa agacttgct caagcgaaa gtggaaggg 
      tttagctga tttcgtcac gttagaatg cgacggcaa 
      agacctaac acccaaact gaaagagct taaagtaca 
      cgcgattgt cttcttaaa cattccaag ttatggtgg 
      gggaacaag gccctggaa ccgatccat gggcacgtg 
      tgagccaag cactgacga cggtttgat tgaagtcgt 
      cggtttgac aacatatca gcttccttt gctacaagc 
      tggaggcca cggtacttg cattacgca atgtaggtc 
      ggcaaaaat cgtaacatc caaaacaat acccatgca 
      agtggatgg ggaagcttg caaattaaa cccttcgat 
      cataaatct ttcactgtt aaataaagc tccaatgct 
      ggcaaagcg gaagtgtgg caaacccaa cgtaccgca 
      aactgctct cgacaatct gaaaatttg tgttcatag 
      aattagcat ggctgacta tgaacagca ccactatga 
      caaggatct attagctca agcggcaat aagcgttgg 
      tacaatcga agttaaccc aatctggga tttggaaca 
      agtgagagc gttaattaa caaatttat agaagactg 
      caaagacca gcaaaaaat ccaccccga gtggtgttt 
      tttagactc atgtacagc tgaacggtt cttccgcat 
      cgacaaggc ccaagaaaa ccttcatta cgtgctgga 
      tatcgcggt cgataactt atacatttt ggaatgcgg 
      aggtggaac tgttcctca aactcccga agacgaaac 
      agccacacc gtctcccag ttctattaa tcaaacatc 
      gtacattat tccagaaat gcgcgttag tgatgaagg 
      cagcctgga ttcgccgag cgatcaacc accgatgag 
      tccaaaaat cctgcaatc tccggaaga atttcccag 
      cgagacaat tgcggaaga gaaccgcaa cggcgacaa 
      gcaattagc gcgcatttc ccccgacgc tagcgtgaa 
      aataagaag ggacttgcc acaaaagcc gaacgaagc 
      tctcttaaa agccgccaa agtggggga cttgaaaac 
      agtcaagga attacacag tcagggcta ctctctact 
      taccaaaga cccagccgg ccaaacaat gttgcatta 
      cgcctcacg gtatggcca caaagatat tgttaaata 
      cttactcca gcacgcccc accttcaat tctgaatat 
      caaagacgt gaacttagg acaaacggc gcttcataa 
      agcagcagc gtacaagtg gcgcagtat ttgttgtat 
      gttagtcgc cggaggcgc agctttgga tattacaga 
      tcgtcgagg tttaactcc gagattatt agcgttaca 
      atccgacga tcacgattt ggctgcata cctcgaaag 
      tcaggaaca ctttcagtt ggtgtcctc cagtttagg 
      gactgttgt atgaaacga gcaataatt gtattaaat 
      tttcctgtc actggtttc tctaaaatt gccttacta 
      cttgtattc aaaattgct aatgtatga ttgtaccta 
      aaatactac aaatgtgat aatactaaa gttaagctt 
      ttttattat ttttttatt tatttgttt cattattgt 
      ttgtttgat aaagttagt taaagtgtt atatttttt 
      cactagaac gtattcttc caggaggag aacaatcat 
      aaagtattg taaataagc gataagtgt ttaaagtta 
      ttgacaaac atatcactc atgacattc ctgtaaagt 
      ttgtagacg taagtgtgt tgaataatt tgttacgtt 
      ttgatctgt tgaatcatt tgcttgagg aaattcgaa 
      cacagggca ataaattag attcgatgt gtctagcat 
      attatccat aaatgttat actctttta ttaaattgt 
      tacgtttta cgtgtacat aagtactgc aacgaattt 
      caatatttc ccgaaaaga caatctgta aatgtgaaa 
      taaatagtt gttgtaagt gctgtcatg tattaatag 
      ttgtataca tttttgtct ctttgaaag ttttattat 
      tcgattttt gaactgcat taagcatct aggatttga 
      aatattaac agtaatagt gtgtaaata tgtagccat 
      taggcatca ctctgtaga taatctagt cttatagtg 
      atacaactg cagaaattt atataaagt gcgttaaca 
      actttttac aaatttctg caattacga tgagtaaat 
      caatgcctt acgaacttt gagtttttt aacagaata 
      gtcaatacg tagctagtt tcgtcacta gaatactct 
      atttatatt tttagtcta ttttatctt ttatcaata 
      ttatctcct agaatcttt gcttttttt aattttatc 
      aattcacgt tgttggtag attttactt aacatcggt 
      tccattcaa gatggaata atctcgctc gatattctt 
      tcactagtt actatgaaa atgtgattg aaactaaat 
      gtacataaa tatttatgt aattacttc ttgtaaatt 
      taatctagt ctttacagg tgtaagttg tagcagtag 
      ttgagggaa cattcttac aatgatagt agatataaa 
      ccacatttt tgccattta aacaccact cagtcagtt 
      cagttgatt gcagtttat tggaaataa aattacctg 
      catacgtac ggttttatt taccttacg ttacgtgaa 
      caacttgta aatacacct gaacattgt gactagtat 
      taaattagg tagagttta atgacttgt tatctagtt 
      atttcatat taatctgac accaaaaac gaagtataa 
      aattattga ttaacaacg tatttgcac gtacccaga 
      gttttaaac ctaggaata attatgcaa cgaatctgg 
      cccagacaa tactcactt tgggccagt ttcgtcttt 
      atattacgc tgcctcgga actaatttc gtgttaatg 
      cgatattat agggttttt ataatcaat gtcgaggaa 
      attagccac attcacgct ctatattat caatttaac 
      cacgaattt tttggtcaa ttatttggt tgagtgagg 
      attttttta acctgtcca tttaggtaa ctaagggtc 
      caagcatgt gtctaggtc tattttaaa aaattccat 
      tttatgtgg taaaacgta ttgggccag ttgtaaatt 
      tgcaaagtt ttctccaac ctcataccg agcactgtt 
      tgtagcaca gtttgcaaa gggtttcta tagcaccat 
      gaccacgcc atatttttg taattacac aagcaaaga 
      tgttttaat aatattgta aagtctctt attgtaaat 
      cgtgtaaaa tggctgaga tttaacttc gatttgatc 
      attttctat gcctaacta tgtattata atattatca 
      aatataaat atatttaag aa
    • >TC000797 RC
      tagaaccgc gataaaggg gcaaaacag ttacagaat 
      gggcgtaga gtgggacaa gggcggcag cagcccccg 
      ctgagtcat gcagcggct caggaccac gtttaaacg 
      gtcccgaac tccgactgg tgccgaaat gaagactca 
      aagcagtct ggaggtgcc caagcaggt gcgctctgc 
      ctctttcga cgagattca gttggaggc gaagcgact 
      gcaatcggg gggtcagtc gaccgaccc ttcggcgct 
      tctcagagt gccccagtt ccccggaca gcgttcgag 
      gagtgtcga ttcgggagg tagcgaaga atccggcaa 
      tttcctcga agtaccggc gcgacgatt tcaacggcg 
      ccgctcctc aagcagcaa aagtccggt ctgtgtgca 
      ctgcgtttg cttggagga gtacgccaa actacatcc 
      ggcggaaga acagccgcc ggaaccgcc ggacgagtt 
      gccgctacc cacttccag catcagcga ctcctccag 
      cgaatgcga agaagaagt cctcccaga atgctccat 
      cacagtcac tctctcccc catactacc tccccctcc 
      catccaatc cccaccacc gcttcctgc atcccctgt 
      catcgaatt tttcccaac tgaagatgt ccaaacacc 
      gaccacgcc aacaaggcg gaaatcaat tcaccgtca 
      agaagcttt tttcgccga accaactgg aacctcttt 
      ggagaatat gtcagatgg tccgtctga actgtactc 
      agaggggac atcggaagg agcactgtc tgaagggga 
      cgctgttaa cgccaccat accgggctt ggtagtccg 
      agatattta cttgcaagt ccctgactt gaaacgcga 
      cagagctgc gtctgttga ttcgtgttt ttcgaaagt 
      ctcgcaagg aaaaaccga ggagttaca acattccgg 
      ggttagttt ggaggtgcc agtggggcc aaatgtggc 
      tttacggtc acggtctgt ggatatcgt attgccaac 
      agatgaaca tgccaggta caaggcctt ggctatggc 
      cgcagcgcc tggcacttc ttcgtctta ttacaaggg 
      gaaagttgt cggcgatgt tggagaaag ggtcctccg 
      gagtactcc ggactgggg cgaaactgc aatcaacgg 
      tgatcattt atgggttcc cacatcggt ctcgggtga 
      cttttgcta catcggaga tatggactg cacgaaaca 
      cggtgcccg gatgaagtg ttccgcttg taatgtggt 
      cgcgcacca gaattgcat cgggatctt gatggataa 
      gatgcgatt tacatgcaa gcctacgtt ccgtgatgt 
      gggagttag acagtacag agaacagac ctccatcca 
      tcaccattg ggtccaccg acggtcaga gaagggtaa 
      atgtcgaca atgcggcaa gtcattcca gtccaagtt 
      atcgtttag ttcgaaaga aatagtcgc aattagttg 
      ttcgtggtg caaggctgc ttatcacaa taaggacac 
      ctgctttaa tatgcaacg aataggcga ggagtgcaa 
      tttaggtgt tcatcgcag cattgtggt tcctccttc 
      gtggatcgt gaaactgcc tcgcaaagg aagtttcaa 
      gtcaagtct gagaaaatc tccgaagaa acgggcttc 
      cagcaagcg aaaaaccaa ggaaaagga taaagagca 
      cagaacgtt cgccatcaa gccgatccc cacaaccaa 
      cgtaacgcc cgtcatagt gttcatcaa ccctaagtc 
      cgggggtaa ccaaggatc aaagttgct ccagaaatt 
      ccagtggtt gttgaatcc gcgacaggt gtttgactt 
      gacacaggg cggtcccaa gatgggtct cgaactgtt 
      caagaaagt acctaatct acgtgttct tgcctgcgg 
      cggagatgg aaccgttgg ttgggtttt aagtgttat 
      cgaccaaat cgggatctc tcctgctcc agcggtcgg 
      cgttcttcc tctaggaac aggaaatga tctggcaag 
      ggcgctcgg atggggtgg gggttatac ggatgaacc 
      aatatcgaa aatcctgtc caatattag cgctagcga 
      gactgtgtt attagatag gtggtcttt agaggttga 
      gaagaaccc gaacgctga ggctaacga aggcggcaa 
      ggataattt gcctctcaa cgttgtaaa taattatta 
      ttcattagg tgtcgatgc tcatattgc cttagaatt 
      ccacgaagc tcgagaagc tcatcccga aaaattcaa 
      ttctagatt aaggaacaa aatgtttta tggccagat 
      gggcggcaa agacttgct caagcgaaa gtggaaggg 
      tttagctga tttcgtcac gttagaatg cgacggcaa 
      agacctaac acccaaact gaaagagct taaagtaca 
      cgcgattgt cttcttaaa cattccaag ttatggtgg 
      gggaacaag gccctggaa ccgatccat gggcacgtg 
      tgagccaag cactgacga cggtttgat tgaagtcgt 
      cggtttgac aacatatca gcttccttt gctacaagc 
      tggaggcca cggtacttg cattacgca atgtaggtc 
      ggcaaaaat cgtaacatc caaaacaat acccatgca 
      agtggatgg ggaagcttg caaattaaa cccttcgat 
      cataaatct ttcactgtt aaataaagc tccaatgct 
      ggcaaagcg gaagtgtgg caaacccaa cgtaccgca 
      aactgctct cgacaatct gaaaatttg tgttcatag 
      aattagcat ggctgacta tgaacagca ccactatga 
      caaggatct attagctca agcggcaat aagcgttgg 
      tacaatcga agttaaccc aatctggga tttggaaca 
      agtgagagc gttaattaa caaatttat agaagactg 
      caaagacca gcaaaaaat ccaccccga gtggtgttt 
      tttagactc atgtacagc tgaacggtt cttccgcat 
      cgacaaggc ccaagaaaa ccttcatta cgtgctgga 
      tatcgcggt cgataactt atacatttt ggaatgcgg 
      aggtggaac tgttcctca aactcccga agacgaaac 
      agccacacc gtctcccag ttctattaa tcaaacatc 
      gtacattat tccagaaat gcgcgttag tgatgaagg 
      cagcctgga ttcgccgag cgatcaacc accgatgag 
      tccaaaaat cctgcaatc tccggaaga atttcccag 
      cgagacaat tgcggaaga gaaccgcaa cggcgacaa 
      gcaattagc gcgcatttc ccccgacgc tagcgtgaa 
      aataagaag gtacttaaa tttcatttt agctttgac 
      ttactttta taccaagcc tctgattga aatcgaata 
      actgaatca gttaggact tcctcaaac ctactcgat 
      tgtaattcg ctagagccc tgtaaatat agttcaagc 
      cttccggcc gtttgacca attttatta tattgcatg 
      tagatttat ccatcaatg tatagtttt atcgaaatc 
      ttgttagca tgtttctag aataaattt cctttaact 
      at