Status
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Gene information
General information
TC004396 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 7147633..7155477
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004396
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02641

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004396 RA
      MSLPVSQYE ALLAEVRGL RRKAQRVQQ LATPLLSNF 
      SSSDCHHQN DAVKLCTSH DMEPLLTSS STNCDDEPW 
      PTLEPITKT SLEVSSVMS FASSSGGGG LLLGSPSLS 
      RRSSQQLEA KVDMVYSLL AMLSGQEHA DMGETLLAL 
      STNPESCLA MRQSGCIPL LVQMVQSDR EPETRTKAA 
      QALHNLVHS QQDEKVKKR EVRVFKLLE HTRGYTDAV 
      RAHKESEFE SEEEDKHPV QTVAHLMKL SFDELHRQA 
      ICTLGGIHT IASLVEAEH SYHGSITTE PHCILMRRY 
      ACMALTNLT FGDSGNKAL LCSYREFMR ALVVQLQSP 
      SDELRQVTA SVLRNLSWR ADSTSKEIL REVGSVSGL 
      MKSAMLDNK ENTLKSILS ALWNLSAHC TENKSEICS 
      VEGALAFLV NTLTYKTPS KSLAIIENS GGILRNISS 
      QIAVRDDYR EILRRHNCL QILLDQLKS PSLTIVSNA 
      CGTLWNLSA KCYTDQELL WQMGAPAML RSLNHSKHK 
      MIAMGSSAA LKNLLSAKP TQLLPSVMD STALAMDLP 
      ALPTLGARK QKALLHDLD QALLSETFD NLEKESPKK 
      CSDNFCSLS EASTSYETR KNLSLPYFD ESSNDLNVG 
      KCFMKKIEN KLNVCSDSG LQSFEVDSG SSSSLDNDK 
      CKRDDEKDH CSIEDVSDS DNQVCDDLS FMGLTVRRE 
      LETTHISPK FKSPPNVTM FEGDIDEID IHDISIHFE 
      EKQDTENEK KIEMSSNKQ KMNNSGIPL PKSLPPRPR 
      QEPIDSPPL LLDTPKKIN RSASALPRP QTTSTPITS 
      SEATKVENI DDRSGEMDE FPFLNHKDE RDRLLERSC 
      EENNPMDNR MLDPDAMIQ SLDRFTAEL VSQASHLQS 
      DEKYKDNTW NEDTSPNEV TFPSISGSA PNVITFESD 
      DIGEKPIES NDFSSVSTM TESTLIAIE ASKMATVFK 
      NEAEMSSST TWLELDSVQ PPSLLESFN SDVGKSPKL 
      TTRKKSFSL VRRALSNSQ NSDSLLDQG NPLELDTTF 
      PEFVINGSI NCNVMTSKL TDLDNINPP SLFNEITDL 
      CNSLSDIAT EAIGSETEI FEDCQTHID DVTEFSDAN 
      SATPIQSDP SSPEITPKK NKTIKKPMT LKEKRHLAK 
      DRYKTYTVA AEMFSQLED ETSDYQSTV EEPKSKSSI 
      RKNSLQKRL ENSDRFKTQ ILSPSPELH SIVAKEANL 
      VLKNLQATK TSDELLDCE TLSLVSNDD DSEQSSVNY 
      RTYHKSWGL KKNNIPVIE ASTKPKIVK PSEDDVQVS 
      PPPPAPPKA IRGRRKPLY SKPAPKSLK PSNLVRNIT 
      TSMKTTGKP VKPKLERQG TFTKEETTS AKISKIPSK 
      IPARNFGRS TSAESAKRV QSASSGKRA SLPQPVPKS 
      ASNATKKQV TSKIASIWK KIEESKKQQ PVKTADTRV 
      WIEAKGGCG SGGGVAMRG TKREGDEGG KRVSRLGSF 
      IVMDDGGEQ N
    CDS sequence
    • >TC004396 RA
      atgtctttg ccggtgtcg caatatgag gctttgctg 
      gcggaagtg cgcggtttg agacgtaaa gctcaacgc 
      gtacaacag ctggcgaca cctcttcta tcgaatttt 
      tcttccagt gactgtcat catcagaat gacgcagta 
      aaattgtgt acttcccac gacatggag cctctactc 
      acctcatcg tcaacaaat tgcgatgat gagccatgg 
      ccaaccttg gagcccatc accaaaacg tcgctcgaa 
      gtcagcagt gtgatgagt ttcgcatcg agttctggt 
      ggtggtggt cttcttctg ggaagtccc agtttatca 
      cgtcgttcc tcccaacaa ttagaagcc aaagttgat 
      atggtttat agtctttta gcaatgtta agtgggcaa 
      gaacatgcc gatatgggt gaaacactt ctagcatta 
      agtacaaac ccagaaagt tgtctagca atgcgtcaa 
      tcgggttgt ataccatta ttagtacaa atggtccaa 
      tcagatcgc gagccagaa acaagaaca aaagctgca 
      caggctctt cataattta gtgcattca cagcaggac 
      gaaaaagtg aaaaaacgt gaagttcgc gtttttaaa 
      ttattagaa cacactagg ggctacact gatgcggta 
      agagcgcac aaagagagc gaatttgag tccgaagag 
      gaggacaaa catccggta cagactgtt gcacatttg 
      atgaaattg tcatttgat gaattgcac agacaagcg 
      atttgtacc ttgggtggg attcatacg attgccagc 
      ttggttgag gcggaacat tcgtatcat ggaagtatt 
      acaacggaa ccacactgt attttaatg cgtcgttac 
      gcctgtatg gctttgaca aatctaact tttggtgat 
      agtggcaat aaggcgtta ctctgttcg tatcgtgaa 
      tttatgagg gctttagtg gtccaatta caaagtcct 
      agcgatgaa ttacgacag gtgacagcg agtgtttta 
      cgtaattta tcatggcgt gccgattcc acaagcaag 
      gaaattcta cgtgaagtt ggcagtgtt agcggttta 
      atgaaatca gcaatgttg gacaacaaa gaaaacact 
      ttaaaatcg attctgtcg gctttatgg aacctatcg 
      gcacattgt accgaaaac aagagcgag atttgttcg 
      gtagagggc gctcttgca ttcctcgtc aatacacta 
      acttacaaa accccctca aaatcgctt gcaattatt 
      gaaaattct ggcggtatt ttacgcaat atttcgagt 
      caaatagcc gttcgtgat gattatcgt gaaatttta 
      cggcgtcat aattgtctg cagatactt ctcgaccaa 
      ttaaaatcg ccaagtttg acaattgtt agtaacgct 
      tgtggtaca ttgtggaat ctttcggct aaatgttac 
      accgatcaa gagttgcta tggcaaatg ggggcgcca 
      gcaatgctt cgaagtctc aaccattcc aaacataaa 
      atgattgcg atgggatca agtgcggct ttgaaaaat 
      ctactaagt gcaaaacca actcaattg ttaccaagt 
      gtgatggat tcaacagcg ttggctatg gatttgcca 
      gcattgcca acgttaggt gcccgtaaa caaaaagca 
      ctattacat gatttagat caggcgtta ctttcggaa 
      actttcgat aatttagaa aaggaatcg ccaaagaaa 
      tgtagtgac aatttttgt agtttaagt gaggcgagt 
      acaagttat gagacgaga aaaaatcta agtttaccg 
      tattttgac gagtcgagt aacgattta aatgttggt 
      aaatgtttt atgaagaaa atcgaaaat aagttgaat 
      gtttgttcg gattcggga ttgcaaagt ttcgaagtt 
      gattcaggt tcgtccagt tcgttagac aatgataaa 
      tgtaaacga gacgatgag aaagatcat tgttccatt 
      gaagatgtt tccgattcg gataatcaa gtttgtgac 
      gatttaagt tttatgggt cttacggtt cgacgcgaa 
      ttggaaaca acgcacatt tcaccgaaa ttcaagtca 
      ccaccgaat gtgacaatg tttgagggt gatattgac 
      gaaattgac atacacgat atttccata cattttgag 
      gaaaaacaa gacaccgaa aatgagaag aaaatagaa 
      atgtcttct aacaaacaa aagatgaac aacagtggt 
      ataccacta ccgaaaagt cttcccccc cgtcctaga 
      caagagcca atcgattca ccaccactg ttacttgat 
      acaccaaag aaaatcaat cgttcagca tcggcttta 
      ccacgacct cagacgacg agtacaccg attacaagc 
      agtgaagcc acaaaagtt gaaaatatc gacgacagg 
      tctggcgag atggacgaa tttccattt ttgaatcac 
      aaagacgaa cgtgatcgt cttttggaa cgcagttgt 
      gaggaaaac aatccaatg gataatcga atgctggat 
      ccggacgct atgattcaa tcgcttgat cgttttaca 
      gccgaatta gtgtcacaa gcctcgcat cttcaaagc 
      gatgaaaaa tacaaagac aatacttgg aatgaagac 
      acatcaccg aatgaagta acatttcca agtatttcc 
      ggtagtgcc ccgaatgtt atcacattc gaatcggac 
      gacatcggg gaaaaacca attgaatca aatgatttt 
      tcatcagtt agtacaatg accgaatca actttgatc 
      gcaatcgaa gcaagtaaa atggcaaca gtgtttaaa 
      aacgaagct gaaatgtcg tcaagtacc acttggctc 
      gaattggat agcgtacaa ccgccgagt ttactcgaa 
      tcgtttaat tccgatgtg ggtaaaagt ccgaaattg 
      acaacgcgg aaaaaatcg tttagtttg gtgcggcgt 
      gcgttgagt aattcccag aattcggat agtttacta 
      gatcagggg aatccctta gaattggat acgacattt 
      cccgagttt gttatcaac ggttcgatt aattgtaat 
      gtgatgacg tccaaattg accgatttg gataatatt 
      aatccaccg tcgttgttt aacgaaatt acagatctc 
      tgtaattcg cttagtgac atagcaacg gaagctatc 
      ggaagcgaa actgaaatt tttgaagat tgccaaaca 
      catattgac gatgttacg gaattttcc gatgctaat 
      agtgccact ccaatacaa tcggatcca agtagtccg 
      gaaattaca ccaaagaaa aacaaaaca atcaaaaaa 
      ccaatgacg ttgaaagag aaaagacac ttagccaaa 
      gatcgatat aaaacttat acagtggca gctgagatg 
      ttttcacaa ttagaggac gaaacaagt gactatcag 
      tcaacagtt gaagaaccc aaaagtaaa tcaagtatt 
      cggaaaaat tccctccag aaacgacta gaaaatagt 
      gatcgtttt aagacacaa attttaagt ccaagtcct 
      gagttgcat tcaattgtg gcaaaggaa gcaaatcta 
      gttttaaaa aatctacaa gcgacaaaa acaagtgac 
      gaattactt gattgtgag actttgagt ttggtttca 
      aacgacgat gattccgaa cagagttcg gtgaattac 
      cgaacctat cacaaaagt tggggtttg aaaaaaaat 
      aatatccca gtgattgaa gcgagcacg aaaccaaaa 
      attgtcaaa ccttctgaa gatgacgtc caggtgtct 
      ccaccacca ccagcgcct ccgaaagca atccgcggt 
      cgtcgcaaa ccactctac tcgaaaccg gctcccaag 
      tcactgaaa ccgtcgaat cttgtccga aatataaca 
      acttcgatg aaaacaacc ggcaaacca gttaagccg 
      aagttggag cgtcaaggg acattcacg aaagaagag 
      acgacgagt gcgaaaatt tcgaaaatt ccgtcgaaa 
      attccagct aggaatttt ggacgatcg acgagtgcg 
      gaaagtgca aagcgagtg caaagcgca agtagtggg 
      aaaagggcg tcgttaccg cagccggtg ccaaaaagt 
      gcgagtaat gcaacaaag aagcaagtg acgagtaaa 
      attgccagt atttggaag aagattgaa gaaagtaag 
      aagcaacag ccagtgaag acggccgat acgagggtt 
      tggattgag gcgaaaggc ggctgtgga agtgggggc 
      ggagtcgcg atgaggggg acgaagcgt gaaggggat 
      gaagggggg aagagggtg tcaaggttg ggctcgttt 
      attgttatg gacgatggg ggggagcag aattga
    mRNA sequence
    • >TC004396 RA
      cgacgggag tgcaattgt atgtgaaaa aagtgtttt 
      tttggatta aaaactccg tcagttctt ttgggattt 
      aattttcat caaacacga tggatgttc gtgatgtct 
      ttgccggtg tcgcaatat gaggctttg ctggcggaa 
      gtgcgcggt ttgagacgt aaagctcaa cgcgtacaa 
      cagctggcg acacctctt ctatcgaat ttttcttcc 
      agtgactgt catcatcag aatgacgca gtaaaattg 
      tgtacttcc cacgacatg gagcctcta ctcacctca 
      tcgtcaaca aattgcgat gatgagcca tggccaacc 
      ttggagccc atcaccaaa acgtcgctc gaagtcagc 
      agtgtgatg agtttcgca tcgagttct ggtggtggt 
      ggtcttctt ctgggaagt cccagttta tcacgtcgt 
      tcctcccaa caattagaa gccaaagtt gatatggtt 
      tatagtctt ttagcaatg ttaagtggg caagaacat 
      gccgatatg ggtgaaaca cttctagca ttaagtaca 
      aacccagaa agttgtcta gcaatgcgt caatcgggt 
      tgtatacca ttattagta caaatggtc caatcagat 
      cgcgagcca gaaacaaga acaaaagct gcacaggct 
      cttcataat ttagtgcat tcacagcag gacgaaaaa 
      gtgaaaaaa cgtgaagtt cgcgttttt aaattatta 
      gaacacact aggggctac actgatgcg gtaagagcg 
      cacaaagag agcgaattt gagtccgaa gaggaggac 
      aaacatccg gtacagact gttgcacat ttgatgaaa 
      ttgtcattt gatgaattg cacagacaa gcgatttgt 
      accttgggt gggattcat acgattgcc agcttggtt 
      gaggcggaa cattcgtat catggaagt attacaacg 
      gaaccacac tgtatttta atgcgtcgt tacgcctgt 
      atggctttg acaaatcta acttttggt gatagtggc 
      aataaggcg ttactctgt tcgtatcgt gaatttatg 
      agggcttta gtggtccaa ttacaaagt cctagcgat 
      gaattacga caggtgaca gcgagtgtt ttacgtaat 
      ttatcatgg cgtgccgat tccacaagc aaggaaatt 
      ctacgtgaa gttggcagt gttagcggt ttaatgaaa 
      tcagcaatg ttggacaac aaagaaaac actttaaaa 
      tcgattctg tcggcttta tggaaccta tcggcacat 
      tgtaccgaa aacaagagc gagatttgt tcggtagag 
      ggcgctctt gcattcctc gtcaataca ctaacttac 
      aaaaccccc tcaaaatcg cttgcaatt attgaaaat 
      tctggcggt attttacgc aatatttcg agtcaaata 
      gccgttcgt gatgattat cgtgaaatt ttacggcgt 
      cataattgt ctgcagata cttctcgac caattaaaa 
      tcgccaagt ttgacaatt gttagtaac gcttgtggt 
      acattgtgg aatctttcg gctaaatgt tacaccgat 
      caagagttg ctatggcaa atgggggcg ccagcaatg 
      cttcgaagt ctcaaccat tccaaacat aaaatgatt 
      gcgatggga tcaagtgcg gctttgaaa aatctacta 
      agtgcaaaa ccaactcaa ttgttacca agtgtgatg 
      gattcaaca gcgttggct atggatttg ccagcattg 
      ccaacgtta ggtgcccgt aaacaaaaa gcactatta 
      catgattta gatcaggcg ttactttcg gaaactttc 
      gataattta gaaaaggaa tcgccaaag aaatgtagt 
      gacaatttt tgtagttta agtgaggcg agtacaagt 
      tatgagacg agaaaaaat ctaagttta ccgtatttt 
      gacgagtcg agtaacgat ttaaatgtt ggtaaatgt 
      tttatgaag aaaatcgaa aataagttg aatgtttgt 
      tcggattcg ggattgcaa agtttcgaa gttgattca 
      ggttcgtcc agttcgtta gacaatgat aaatgtaaa 
      cgagacgat gagaaagat cattgttcc attgaagat 
      gtttccgat tcggataat caagtttgt gacgattta 
      agttttatg ggtcttacg gttcgacgc gaattggaa 
      acaacgcac atttcaccg aaattcaag tcaccaccg 
      aatgtgaca atgtttgag ggtgatatt gacgaaatt 
      gacatacac gatatttcc atacatttt gaggaaaaa 
      caagacacc gaaaatgag aagaaaata gaaatgtct 
      tctaacaaa caaaagatg aacaacagt ggtatacca 
      ctaccgaaa agtcttccc ccccgtcct agacaagag 
      ccaatcgat tcaccacca ctgttactt gatacacca 
      aagaaaatc aatcgttca gcatcggct ttaccacga 
      cctcagacg acgagtaca ccgattaca agcagtgaa 
      gccacaaaa gttgaaaat atcgacgac aggtctggc 
      gagatggac gaatttcca tttttgaat cacaaagac 
      gaacgtgat cgtcttttg gaacgcagt tgtgaggaa 
      aacaatcca atggataat cgaatgctg gatccggac 
      gctatgatt caatcgctt gatcgtttt acagccgaa 
      ttagtgtca caagcctcg catcttcaa agcgatgaa 
      aaatacaaa gacaatact tggaatgaa gacacatca 
      ccgaatgaa gtaacattt ccaagtatt tccggtagt 
      gccccgaat gttatcaca ttcgaatcg gacgacatc 
      ggggaaaaa ccaattgaa tcaaatgat ttttcatca 
      gttagtaca atgaccgaa tcaactttg atcgcaatc 
      gaagcaagt aaaatggca acagtgttt aaaaacgaa 
      gctgaaatg tcgtcaagt accacttgg ctcgaattg 
      gatagcgta caaccgccg agtttactc gaatcgttt 
      aattccgat gtgggtaaa agtccgaaa ttgacaacg 
      cggaaaaaa tcgtttagt ttggtgcgg cgtgcgttg 
      agtaattcc cagaattcg gatagttta ctagatcag 
      gggaatccc ttagaattg gatacgaca tttcccgag 
      tttgttatc aacggttcg attaattgt aatgtgatg 
      acgtccaaa ttgaccgat ttggataat attaatcca 
      ccgtcgttg tttaacgaa attacagat ctctgtaat 
      tcgcttagt gacatagca acggaagct atcggaagc 
      gaaactgaa atttttgaa gattgccaa acacatatt 
      gacgatgtt acggaattt tccgatgct aatagtgcc 
      actccaata caatcggat ccaagtagt ccggaaatt 
      acaccaaag aaaaacaaa acaatcaaa aaaccaatg 
      acgttgaaa gagaaaaga cacttagcc aaagatcga 
      tataaaact tatacagtg gcagctgag atgttttca 
      caattagag gacgaaaca agtgactat cagtcaaca 
      gttgaagaa cccaaaagt aaatcaagt attcggaaa 
      aattccctc cagaaacga ctagaaaat agtgatcgt 
      tttaagaca caaatttta agtccaagt cctgagttg 
      cattcaatt gtggcaaag gaagcaaat ctagtttta 
      aaaaatcta caagcgaca aaaacaagt gacgaatta 
      cttgattgt gagactttg agtttggtt tcaaacgac 
      gatgattcc gaacagagt tcggtgaat taccgaacc 
      tatcacaaa agttggggt ttgaaaaaa aataatatc 
      ccagtgatt gaagcgagc acgaaacca aaaattgtc 
      aaaccttct gaagatgac gtccaggtg tctccacca 
      ccaccagcg cctccgaaa gcaatccgc ggtcgtcgc 
      aaaccactc tactcgaaa ccggctccc aagtcactg 
      aaaccgtcg aatcttgtc cgaaatata acaacttcg 
      atgaaaaca accggcaaa ccagttaag ccgaagttg 
      gagcgtcaa gggacattc acgaaagaa gagacgacg 
      agtgcgaaa atttcgaaa attccgtcg aaaattcca 
      gctaggaat tttggacga tcgacgagt gcggaaagt 
      gcaaagcga gtgcaaagc gcaagtagt gggaaaagg 
      gcgtcgtta ccgcagccg gtgccaaaa agtgcgagt 
      aatgcaaca aagaagcaa gtgacgagt aaaattgcc 
      agtatttgg aagaagatt gaagaaagt aagaagcaa 
      cagccagtg aagacggcc gatacgagg gtttggatt 
      gaggcgaaa ggcggctgt ggaagtggg ggcggagtc 
      gcgatgagg gggacgaag cgtgaaggg gatgaaggg 
      gggaagagg gtgtcaagg ttgggctcg tttattgtt 
      atggacgat gggggggag cagaattga gtgagggtg 
      ttaaaatca atgccataa atatgtctc tagtctgat 
      taattattt tatgtaaag attatgtac ctatattta 
      tttagattt ttttttgta gaagtgtat ataaaaaaa 
      tcatgttat tacgtcaag tagtttttt aatatttgt 
      aataatcat tcgactacg atttctcaa gacggaaat 
      tctttaaaa ttgtactta tgtaacaac tgatttgtg 
      aataaatta ctcactttc at
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