Status
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Gene information
General information
TC031414
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC016235
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 4813229..4824792
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031414
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07166

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_02640

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031414 RA
      MIPIILTIT VVTIVYYLL HLNYQYWKK RGIANPKPV 
      FLLGNFGKC FFLRASPGQ VYYDVYNKY KNEQVVGLY 
      RATTPVLLV RDPELIKEV MVKSFSSFH NNDVYVYKK 
      RDPIVGRNP FVLKGEEWK SVRQQLTPG FTSGKMKWL 
      YPLLDEVAE NLVKFIENQ PKTLNAKDV CARFTLNNV 
      ASCAFGIEG KCFEEENNH FRQMAIEFL SPSSWSFIG 
      LFLVLLNPS LLKVLPIKF VTKNCEKKL IDLVQQTVK 
      YREDNNVVR NDFLHILTQ LKKTSSEFT DIDVVAHAA 
      GFFGDGYET TAGVMSFVL YCLAENLDI QTKLRELVD 
      KVFENNEEK LPYEALQEI HFLDAVVNE TLRLHPPAL 
      HFQKLCTQD FTFVLKNAN KSVTIEKGT PVILPVYGL 
      HHDPDYYES PDLFKPERF LPENKDKTV KCTFMPFGE 
      GPRSCLGQR FGLLQVKVG VAHIIRHFE LSVNGKTQR 
      PIKYEPTNP VTAPVGGFW DAILKALVN YHPEKTRYC 
      SRLSTGKML PIILIVTAV TIICYLIHL NYQYWKKRG 
      VKGPRPHYL VGNIGKVLL LQASPGQVF AEAYNEFKD 
      EDVVGLYRA MSPIILIRD PELVKEVIL KSFNSFHDN 
      DMYIDKGTD AIAGRHPFL LRGDDWKIT RQKLTPGFT 
      SGKMKWLYP LLEEVSATL IKFIETHPE TTNGTGCDI 
      KDLCGRFTL NNVASCAFG IEGKCLEEK NSQFQQLAL 
      DFMSPGSTA FFTFFLSTI IPSLSKILK VRFISQDVE 
      NRLVDLIGQ TVKYRQENN VLRNDFLHI LIELTKTHK 
      DYQLIDVTS HAAAFFADG YETSASVMS FVLYELAAN 
      PGVQERLRE EVGEAFAKN GTLPYDGLQ EIHFLDAIV 
      SETLRLHPP LQHLQKVCT KDFTFVAKN TNKTVTIEK 
      GTTTIIPIY GLQHDSKFF ENPDSFQPE RFLGSNKDS 
      IAKGTFLPY GDGPRACLG IRFGLLQIK VGVAYIIHH 
      YELTVNKKT KVPLKFVAT SPVTAPVGG FWLNFRKLK
    CDS sequence
    • >TC031414 RA
      atgatccca ataatccta acaatcaca gtggtgaca 
      atagtgtac tatttgctg cacctcaac taccagtac 
      tggaaaaaa aggggaatc gccaaccca aagcccgta 
      tttttgctc ggcaatttt ggaaaatgc ttctttttg 
      cgagcgtca ccaggccaa gtgtattac gatgtttac 
      aataaatat aaaaacgaa caagtggtc ggactgtat 
      cgagcaacc acgcctgta cttctagtt cgtgatcct 
      gaactaatt aaggaagtg atggtaaag tcgtttagc 
      agtttccat aataatgat gtgtacgtt tacaaaaaa 
      agggaccca attgttggg cgaaaccct ttcgtcttg 
      aagggagag gagtggaaa agtgttcgg cagcagttg 
      acgccagga ttcacaagt ggaaagatg aaatggctg 
      taccctctc ttagacgaa gtggccgaa aacctggtc 
      aaattcatc gaaaatcag cccaaaact ctcaacgcc 
      aaagacgtg tgcgcccgt tttacgcta aataacgtg 
      gcaagttgt gctttcgga attgaaggc aaatgcttc 
      gaggaggaa aataaccac ttcagacaa atggccatt 
      gagttcctc tcgcccagt agttggagc ttcattggt 
      ctgttcctc gtattactg aacccgtcc ttgctcaaa 
      gtcctccca ataaaattc gtgacgaaa aactgcgag 
      aaaaaactg atcgacttg gtccaacaa acggtaaag 
      taccgcgaa gacaacaat gttgtccgc aacgatttc 
      ctccacatt ttgacacaa ctgaagaaa acctccagc 
      gagttcacg gacattgac gtggtggca catgcagct 
      gggtttttc ggcgatggc tatgagact actgctggg 
      gttatgagt tttgttttg tattgtttg gcggaaaac 
      ttagacatt cagacaaaa ttgcgagaa ttagtcgat 
      aaagtgttt gaaaataac gaggaaaaa ttgccgtat 
      gaagccttg caagaaatt cacttcttg gatgctgta 
      gttaacgag acactacgt ttgcatccg ccggctctc 
      cacttccaa aaattgtgc acacaggac tttacgttc 
      gttttgaaa aatgcaaac aagtcggtg actattgag 
      aaaggcact ccggtcatt ttgcccgtt tatggcctc 
      catcatgat ccggattat tacgaaagt ccggatttg 
      ttcaagccg gagcgcttt ctcccggaa aataaggac 
      aaaacggtc aagtgcacg tttatgcct tttggtgaa 
      ggcccccgg agttgttta ggacaaagg ttcggtttg 
      ttgcaagtt aaagtcgga gttgcgcat attattcgc 
      cattttgag ttgagtgtc aatgggaaa acgcaaagg 
      ccgataaag tacgaacca acgaatcct gtgacggca 
      ccggttggc ggattttgg gacgcaatt ctcaaggcc 
      ctagttaat tatcatcca gagaaaact cgttattgc 
      agtcgtctc tcaaccggc aaaatgcta ccaataatc 
      ctgatagtc acagctgtg acaataatt tgctactta 
      atacacttg aattaccag tattggaaa aaacgaggt 
      gtgaaaggc ccaagaccg cactatttg gtcggaaat 
      atcggcaaa gtgctactt ttgcaagcc tcaccagga 
      caagttttc gccgaagct tacaatgaa tttaaagat 
      gaagacgtg gtgggtttg tatagagcc atgtcacct 
      ataatttta attcgtgac cctgagctg gtcaaagag 
      gtgattctt aagtcgttt aatagcttc cacgataat 
      gatatgtac atagataag ggtactgat gctattgca 
      gggcggcat ccctttctg ttaaggggg gatgattgg 
      aaaattaca aggcaaaaa ctaacacct ggattcacc 
      agtggaaaa atgaaatgg ttgtaccct cttttagaa 
      gaagtaagt gctacttta atcaaattc atcgaaacc 
      caccccgaa acaacaaac ggaaccggt tgcgacatc 
      aaagacctc tgcggccgc ttcaccctc aacaacgtc 
      gccagctgt gccttcggc atcgaaggc aaatgcctc 
      gaagagaaa aacagccaa ttccagcaa ctggccctc 
      gacttcatg tcaccagga agcacagcc ttcttcacc 
      ttcttcctc tccacgatc atcccctcc ctctcgaaa 
      atcctcaaa gtgcggttc atctcccaa gacgtcgaa 
      aaccgactc gtcgacctc atcggccaa acggtaaag 
      taccggcag gagaacaac gtgctccgg aacgacttc 
      ctccacatt ttgatcgaa ttgacgaaa acccacaag 
      gattaccaa ctgattgac gtgacctcg cacgcagct 
      gcgtttttc gccgacggt tatgagacc agtgcgtcg 
      gtgatgagc tttgtgctg tacgaactg gccgccaat 
      cccggcgtc caggagcgg ctgagggaa gaggtgggc 
      gaagcgttt gcaaaaaat gggacttta ccgtacgat 
      ggcttgcaa gaaattcac tttttggac gcgattgtg 
      agcgaaacg ttgcgtttg catccgcca ttgcaacac 
      ttgcaaaaa gtttgtacg aaagatttt actttcgtg 
      gccaaaaat acgaacaaa acggtgacg attgaaaaa 
      gggacaact acgatcatc ccgatttat gggctccaa 
      catgattcg aaatttttc gaaaatccc gattccttc 
      caaccggag cggtttttg ggctctaat aaggatagt 
      attgccaaa gggacgttt ttgccgtat ggtgatgga 
      ccaagggct tgtttaggg atcaggttt ggtttgttg 
      caaattaaa gtcggagtg gcgtatatt atacaccat 
      tatgagctg actgttaat aagaagacc aaagtgcct 
      ttgaaattt gtggctaca agtcctgta acagcgcct 
      gttggagga ttttggctt aattttaga aaattaaag 
      taa
    mRNA sequence
    • >TC031414 RA
      tttggaaca aattgatca aaattttag ttaaagttt 
      tgttgtctg aaagaacca ttcctccaa tcgctgcaa 
      aatgatccc aataatcct aacaatcac agtggtgac 
      aatagtgta ctatttgct gcacctcaa ctaccagta 
      ctggaaaaa aaggggaat cgccaaccc aaagcccgt 
      atttttgct cggcaattt tggaaaatg cttcttttt 
      gcgagcgtc accaggcca agtgtatta cgatgttta 
      caataaata taaaaacga acaagtggt cggactgta 
      tcgagcaac cacgcctgt acttctagt tcgtgatcc 
      tgaactaat taaggaagt gatggtaaa gtcgtttag 
      cagtttcca taataatga tgtgtacgt ttacaaaaa 
      aagggaccc aattgttgg gcgaaaccc tttcgtctt 
      gaagggaga ggagtggaa aagtgttcg gcagcagtt 
      gacgccagg attcacaag tggaaagat gaaatggct 
      gtaccctct cttagacga agtggccga aaacctggt 
      caaattcat cgaaaatca gcccaaaac tctcaacgc 
      caaagacgt gtgcgcccg ttttacgct aaataacgt 
      ggcaagttg tgctttcgg aattgaagg caaatgctt 
      cgaggagga aaataacca cttcagaca aatggccat 
      tgagttcct ctcgcccag tagttggag cttcattgg 
      tctgttcct cgtattact gaacccgtc cttgctcaa 
      agtcctccc aataaaatt cgtgacgaa aaactgcga 
      gaaaaaact gatcgactt ggtccaaca aacggtaaa 
      gtaccgcga agacaacaa tgttgtccg caacgattt 
      cctccacat tttgacaca actgaagaa aacctccag 
      cgagttcac ggacattga cgtggtggc acatgcagc 
      tgggttttt cggcgatgg ctatgagac tactgctgg 
      ggttatgag ttttgtttt gtattgttt ggcggaaaa 
      cttagacat tcagacaaa attgcgaga attagtcga 
      taaagtgtt tgaaaataa cgaggaaaa attgccgta 
      tgaagcctt gcaagaaat tcacttctt ggatgctgt 
      agttaacga gacactacg tttgcatcc gccggctct 
      ccacttcca aaaattgtg cacacagga ctttacgtt 
      cgttttgaa aaatgcaaa caagtcggt gactattga 
      gaaaggcac tccggtcat tttgcccgt ttatggcct 
      ccatcatga tccggatta ttacgaaag tccggattt 
      gttcaagcc ggagcgctt tctcccgga aaataagga 
      caaaacggt caagtgcac gtttatgcc ttttggtga 
      aggcccccg gagttgttt aggacaaag gttcggttt 
      gttgcaagt taaagtcgg agttgcgca tattattcg 
      ccattttga gttgagtgt caatgggaa aacgcaaag 
      gccgataaa gtacgaacc aacgaatcc tgtgacggc 
      accggttgg cggattttg ggacgcaat tctcaaggc 
      cctagttaa ttatcatcc agagaaaac tcgttattg 
      cagtcgtct ctcaaccgg caaaatgct accaataat 
      cctgatagt cacagctgt gacaataat ttgctactt 
      aatacactt gaattacca gtattggaa aaaacgagg 
      tgtgaaagg cccaagacc gcactattt ggtcggaaa 
      tatcggcaa agtgctact tttgcaagc ctcaccagg 
      acaagtttt cgccgaagc ttacaatga atttaaaga 
      tgaagacgt ggtgggttt gtatagagc catgtcacc 
      tataatttt aattcgtga ccctgagct ggtcaaaga 
      ggtgattct taagtcgtt taatagctt ccacgataa 
      tgatatgta catagataa gggtactga tgctattgc 
      agggcggca tccctttct gttaagggg ggatgattg 
      gaaaattac aaggcaaaa actaacacc tggattcac 
      cagtggaaa aatgaaatg gttgtaccc tcttttaga 
      agaagtaag tgctacttt aatcaaatt catcgaaac 
      ccaccccga aacaacaaa cggaaccgg ttgcgacat 
      caaagacct ctgcggccg cttcaccct caacaacgt 
      cgccagctg tgccttcgg catcgaagg caaatgcct 
      cgaagagaa aaacagcca attccagca actggccct 
      cgacttcat gtcaccagg aagcacagc cttcttcac 
      cttcttcct ctccacgat catcccctc cctctcgaa 
      aatcctcaa agtgcggtt catctccca agacgtcga 
      aaaccgact cgtcgacct catcggcca aacggtaaa 
      gtaccggca ggagaacaa cgtgctccg gaacgactt 
      cctccacat tttgatcga attgacgaa aacccacaa 
      ggattacca actgattga cgtgacctc gcacgcagc 
      tgcgttttt cgccgacgg ttatgagac cagtgcgtc 
      ggtgatgag ctttgtgct gtacgaact ggccgccaa 
      tcccggcgt ccaggagcg gctgaggga agaggtggg 
      cgaagcgtt tgcaaaaaa tgggacttt accgtacga 
      tggcttgca agaaattca ctttttgga cgcgattgt 
      gagcgaaac gttgcgttt gcatccgcc attgcaaca 
      cttgcaaaa agtttgtac gaaagattt tactttcgt 
      ggccaaaaa tacgaacaa aacggtgac gattgaaaa 
      agggacaac tacgatcat cccgattta tgggctcca 
      acatgattc gaaattttt cgaaaatcc cgattcctt 
      ccaaccgga gcggttttt gggctctaa taaggatag 
      tattgccaa agggacgtt tttgccgta tggtgatgg 
      accaagggc ttgtttagg gatcaggtt tggtttgtt 
      gcaaattaa agtcggagt ggcgtatat tatacacca 
      ttatgagct gactgttaa taagaagac caaagtgcc 
      tttgaaatt tgtggctac aagtcctgt aacagcgcc 
      tgttggagg attttggct taattttag aaaattaaa 
      gtaaggacc cggtttgaa tttttggta ctaatgtta 
      agcactgtt ttttatttt tataatcgt gcacaatgt 
      tttggaagt gaataaagg atttgttaa tga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY