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Gene information
General information
TC016231 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid75: 279359..281022
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC016231
    iBeetle screen iB_02639
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    ovary size decreased - Number of animals showing the phenotype: 3

    vitellogenic egg chamber late not present - Number of animals showing the phenotype: 3
    vitellogenic egg chamber early degenerated - Number of animals showing the phenotype: 3

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC016231 RA
      MLPDGETPS CSETTASGT KLGTCEVCA RKNAKYCCP 
      RCEVKTCSL SCNKIHKLE VECSGVRDR AKFIPVNKF 
      TNLDLSSDY RLLEEISRV VETSKKGRS SFGYNFTKG 
      LLRLQQEAL KRHITLKYL PRSFVRHKN NTTRFDFKS 
      QVIEWRVDW VFVNCENLK ISEDKVPEN LRLSKILDK 
      YLSKQGDEA LQYYQAANL PGLRILLKA ELKSGKKFY 
      ELDPSYTLR ECLKNRVII EYPTIHLVL KDQAIFYDI 
      VDSDDEEDT KAELQKQIK SGQEVINKI IKKSESDDS 
      LYESLRNLL FINEYSDEE QGSDEEAK
    CDS sequence
    • >TC016231 RA
      atgcttcct gacggggaa accccgtcc tgttcggaa 
      acaacagca tcaggcaca aaattgggc acatgtgag 
      gtctgtgcg cgcaaaaac gccaagtat tgttgcccc 
      cgatgcgag gtgaagacc tgttcgctc agttgcaat 
      aaaatccac aagctggag gtcgagtgt agtggcgtg 
      agagacagg gctaagttc atccctgtt aataagttc 
      acaaatttg gatttgtcg agcgattat cgcttgttg 
      gaggagatt tcacgcgtg gttgagact tcgaaaaag 
      gggcgctcg agttttggt tacaatttc acgaagggg 
      ttgttgcgt ttgcaacag gaggctcta aagaggcac 
      attacgttg aaatatctc cctcggagt tttgtgcgt 
      cataagaac aacaccact cgttttgat ttcaagtca 
      caagtgatt gagtggcgt gttgattgg gtgtttgtc 
      aattgcgaa aacttgaaa atcagtgaa gataaagtg 
      cctgagaat ttgagactt agtaaaatt cttgataag 
      tatttgagt aagcaagga gatgaggca ctgcagtat 
      tatcaagct gcaaatttg cctggtctt agaatattg 
      ctaaaagcg gaattaaaa tcagggaaa aagttctat 
      gagttggat ccgagctac acactgaga gagtgtttg 
      aaaaacagg gtgataatc gaatatcca actattcat 
      ctagtctta aaagaccaa gcaattttt tacgatatt 
      gttgattca gatgatgag gaggatacg aaagccgag 
      ttgcaaaaa cagataaag tcgggtcag gaagttatt 
      aataaaatt atcaaaaaa tcggaaagt gatgatagt 
      ttatacgaa tctctgagg aatttgttg tttattaat 
      gagtattcg gatgaagag cagggctct gatgaagag 
      gcaaagtga
    mRNA sequence
    • >TC016231 RA
      accttgtct gagtttatt ttggttacg gatgaacta 
      ttttttaag attgtgctg ttttaacga tatgtttct 
      ctacaatta ttacgttat cttatctta tcactgaac 
      acaaaacaa ttgaacacc cgcactaga aaaattaaa 
      gaggcgact ggatggaat gaaattagt cattttacg 
      accaaccaa tacagcttc ttgagactt aattagctg 
      gttaaaagg gcaagcacc gcatttccg gggcttgac 
      cccagaatt gtgagaagt tttaacttg cacttgttt 
      ttttgtgcg atttttggg caaaatttg cgctaatta 
      tttaattgt ggacgtcag tgatgccaa cattgaatt 
      tgaaattat caagtgtca aggttattt tttagttta 
      taatcgttt ttcttcaaa attctccgt ttttggtgc 
      aaatttccc acttgtcat gcttcctga cggggaaac 
      cccgtcctg ttcggaaac aacagcatc aggcacaaa 
      attgggcac atgtgaggt ctgtgcgcg caaaaacgc 
      caagtattg ttgcccccg atgcgaggt gaagacctg 
      ttcgctcag ttgcaataa aatccacaa gctggaggt 
      cgagtgtag tggcgtgag agacagggc taagttcat 
      ccctgttaa taagttcac aaatttgga tttgtcgag 
      cgattatcg cttgttgga ggagatttc acgcgtggt 
      tgagacttc gaaaaaggg gcgctcgag ttttggtta 
      caatttcac gaaggggtt gttgcgttt gcaacagga 
      ggctctaaa gaggcacat tacgttgaa atatctccc 
      tcggagttt tgtgcgtca taagaacaa caccactcg 
      ttttgattt caagtcaca agtgattga gtggcgtgt 
      tgattgggt gtttgtcaa ttgcgaaaa cttgaaaat 
      cagtgaaga taaagtgcc tgagaattt gagacttag 
      taaaattct tgataagta tttgagtaa gcaaggaga 
      tgaggcact gcagtatta tcaagctgc aaatttgcc 
      tggtcttag aatattgct aaaagcgga attaaaatc 
      agggaaaaa gttctatga gttggatcc gagctacac 
      actgagaga gtgtttgaa aaacagggt gataatcga 
      atatccaac tattcatct agtcttaaa agaccaagc 
      aatttttta cgatattgt tgattcaga tgatgagga 
      ggatacgaa agccgagtt gcaaaaaca gataaagtc 
      gggtcagga agttattaa taaaattat caaaaaatc 
      ggaaagtga tgatagttt atacgaatc tctgaggaa 
      tttgttgtt tattaatga gtattcgga tgaagagca 
      gggctctga tgaagaggc aaagtgaac gaaacgggt 
      tcatgttag aaaaacgac gttaaattt ccattgggg 
      tattattaa gacattgag aaacaatgc tttattttt 
      acagtgtgt tatttaatt tttgttaca aataaattg 
      gaagcatct ga