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Gene information
General information
TC031402 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid75: 1399030..1401977
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031402
    iBeetle screen iB_02636
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 3
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031402 RA
      MSEQIRIYF DFVLPLVKK AGKVILEAK NIEIETKDE 
      IYDLVTIYD RKVEEVLIK EIKAKYPNH KFIGEEESS 
      ARSEISDLT NDPTWIIDP IDGTANFVR NFPITCISI 
      GLTINKQPV LGIIYNPFM DELYTAMKG QGAHLNGKR 
      IFTSKQTDI QKSVLNYEL SLARSEKHR ELYMYRLTH 
      LIAVIQGIR SMGSAALGL CYVARGSMD AYQCDGLYP 
      WDAAAGVLI VTEAGGAVG DSTWGKEFD LMKPNFIAA 
      ASKPLLDQF LQVEKRADD ERLAALLVK KANVGCK
    CDS sequence
    • >TC031402 RA
      atgagtgaa cagatccga atttatttc gacttcgtc 
      ctgcctctg gtcaaaaag gccggaaag gtcatcctc 
      gaagccaaa aatatcgaa atcgaaacc aaggacgaa 
      atctacgat ttggtcaca atctacgac cggaaagtc 
      gaagaagtg ctcatcaaa gaaatcaag gcaaaatac 
      cccaatcac aagtttatt ggagaggaa gaatcatca 
      gcgcggagc gaaatttcg gatttgacc aacgacccg 
      acctggatt atagacccc atcgatggg acggccaat 
      ttcgtgcga aattttccc atcacgtgt atttccata 
      gggctcacg atcaacaaa caaccagtg ctgggcatt 
      atctacaac cccttcatg gacgaactc tacacggcc 
      atgaaggga caaggggcg cacttgaac ggaaaacgc 
      atcttcacg agcaaacaa acagatatc caaaaatcg 
      gtcctcaac tacgagcta tccttggcc cggtccgaa 
      aaacaccgc gagttatac atgtacaga ctgacccac 
      ttgattgcg gtgatccag ggaatacgt tcgatgggt 
      tcggccgct ttggggctg tgttacgtg gcgcggggc 
      tcaatggac gcctaccag tgcgatggg ttgtaccca 
      tgggacgcc gctgccggg gttttgata gtaacggag 
      gccggtggg gctgtcggc gactccact tggggtaaa 
      gagttcgat ctcatgaaa ccgaatttc atagcagcg 
      gcctcaaag ccccttttg gatcagttc cttcaagtc 
      gaaaaacgt gccgatgac gaaagattg gctgcactg 
      ctggttaag aaggccaat gttggatgc aaatag
    mRNA sequence
    • >TC031402 RA
      ccggggaaa gattaggaa aaaattgaa agtcaatta 
      gtgattcaa ggacttgtt tgtatttct ctcagtgta 
      cgttcagtg ctcgagtga accaagatg agtgaacag 
      atccgaatt tatttcgac ttcgtcctg cctctggtc 
      aaaaaggcc ggaaaggtc atcctcgaa gccaaaaat 
      atcgaaatc gaaaccaag gacgaaatc tacgatttg 
      gtcacaatc tacgaccgg aaagtcgaa gaagtgctc 
      atcaaagaa atcaaggca aaatacccc aatcacaag 
      tttattgga gaggaagaa tcatcagcg cggagcgaa 
      atttcggat ttgaccaac gacccgacc tggattata 
      gaccccatc gatgggacg gccaatttc gtgcgaaat 
      tttcccatc acgtgtatt tccataggg ctcacgatc 
      aacaaacaa ccagtgctg ggcattatc tacaacccc 
      ttcatggac gaactctac acggccatg aagggacaa 
      ggggcgcac ttgaacgga aaacgcatc ttcacgagc 
      aaacaaaca gatatccaa aaatcggtc ctcaactac 
      gagctatcc ttggcccgg tccgaaaaa caccgcgag 
      ttatacatg tacagactg acccacttg attgcggtg 
      atccaggga atacgttcg atgggttcg gccgctttg 
      gggctgtgt tacgtggcg cggggctca atggacgcc 
      taccagtgc gatgggttg tacccatgg gacgccgct 
      gccggggtt ttgatagta acggaggcc ggtggggct 
      gtcggcgac tccacttgg ggtaaagag ttcgatctc 
      atgaaaccg aatttcata gcagcggcc tcaaagccc 
      cttttggat cagttcctt caagtcgaa aaacgtgcc 
      gatgacgaa agattggct gcactgctg gttaagaag 
      gccaatgtt ggatgcaaa tagtttttt ttattagtt 
      gtaattata attatgcaa taaacttaa ggaaattgg