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Gene information
General information
TC011374 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid105: 351741..371836
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011374
    iBeetle screen iB_02634
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011374 RA
      MTTQRLIFA LLACCSVRM VLAGLCEVD SGQSNIILD 
      IEESRGKSI SQETVPAEL PIVGEPNVD IFLDLAFPK 
      GPALFFLNG KKLQLLAPL DRDENSLSH IVFQLICTI 
      KSTHKKRTV PVIVRLSDI NDNAPEFVN TPYETSISE 
      LTPVGTTIF QDILAKDKD AGVNGLVEY SIVKGENLA 
      HEDDDRIHS EDGFGYFAI NLPHQGQVT VNRTLDFEK 
      TQRYYVTVV ATDRAHDDT QRLSSTTTL VVNIKDDDD 
      QNPSFIYKG CMLLDGTCI NPEYSASVS SGVLHGILN 
      ISPEKIQAV DMDTINSPI HYSFVSGTP ESYSEYFQI 
      DSETGAVRQ IKAVDTSTT KQFNIIIKA QEISEAKRS 
      TTAKLFITV KPVDANPPE IELSSTEGY VNENSPIGE 
      KVLDSEGVP LLISVTDKD FGPNDPKPS YTFELTTPF 
      FTIDKMGYL LVNENNLDR DPPNPGRFK FQIVAREKG 
      GTAASAPMS VTVHLNDVN DNAPMLPII APVGVPAGD 
      VKRQVATIK ATDNDEGDN ALVTYSIYH VSNNGNNKF 
      RIDPNTGVL ETVGKLNAG DQYSLTVQA TDKGGLYSQ 
      AIVEVTISP GPNTQSPIF EQSVYEIEV SEGATINST 
      VATITAMDP ENDPVTYSI VSGNDLRQF SIGAKSGVI 
      TVIRKLDRE DLTRYKLII KAEDAGKLS STATVNIKV 
      TDINDKNPE FVGDPYNFS VKEGLNSTS VGFVKATDA 
      DEGINALVT YSIPSGLPF DIDNETGEI RTNRALDYE 
      TQKNYQFVV TAKDGAPDP RLATATVSI SVIDIEDEL 
      PIFTKSLYT ASVPENIPD YFITDVKAE DPDTIKKIT 
      YVIHQGPTD LFRIDENSG AVYTIRGLD YEKDSQHVL 
      TIGTLENMS NEEGSTTKV IINVEDRND IPPVFTIIP 
      HPITLDDDV AIGTPVTNL VATDSDGTA PGNKVRYEI 
      IGRGKATKY FQIDSDTGI LKVTNDLKK EIDTEYQVD 
      VRAYDLGEP QLSSVITLD IYIQHVATV APEVGLKFA 
      DTSYHVQVP ENATSGTKV KTLTIVNSR THGGNIPLK 
      CLIISGNEQ RKFIVNVTE DRNCALYLN GTIDFETDE 
      SYQLEVQIM SLQGFINKE FSITLVTIN VVDVNDNVP 
      YFIFPSGGT HKYHAAITD NSPLSTTVT QIKADDKDS 
      GKYGKLEFS LDKTEYFTI DSSTGIVKT RKTFDDVDN 
      SELPFKLIV KTRDNPNAT EYNEIKAPF IVNLITKDN 
      RLVLVIGDA KPDIVQKKL PLIIDIMQE QTGLIVGVE 
      KLATREFIG TNGTLETDP SGTDVWFYV IDPTTEKIL 
      PRNSTLVKR AIFDKQAMD NITFDITGQ IQNTAFHIH 
      EPLVLQKIK SASIASFNG EVFPYALII IACIIFVLG 
      IVGIIYICV SWSRYKSYK DQMQRQYVV PASPTRYDT 
      VYVEPNLKE YETQVLQMC VPVDEQDDY NDLHLDFSN 
      KNHAFSLDN VSYISKDNN LKKYGGQSP VSSESATTV 
      RASSVGEGH VLKYRSDDD EMNASPSND NVMFKEKKD 
      YSHLGFSYD HSPVETTTE L
    CDS sequence
    • >TC011374 RA
      atgacgacc caacgcctg atttttgca ctcctggct 
      tgttgcagc gttcggatg gtacttgca gggttgtgt 
      gaagtggac tcgggtcaa tcgaacata atcctggac 
      attgaagaa agtcgcgga aaatcaatt agccaagag 
      accgttcct gcagagtta ccaattgtt ggcgagcca 
      aatgtcgat attttcctc gatttggcg tttcccaaa 
      gggcctgcg ctttttttt ctcaatggg aaaaagctg 
      caattgttg gcgccgtta gatcgagac gaaaacagt 
      ctctcgcat attgttttt cagttaatt tgcacaatt 
      aaatcgact cacaagaaa cgaactgta ccagtaatt 
      gtaagactg agtgatatt aacgataat gcaccagaa 
      ttcgtcaac acgccgtac gagacgagt atatcggag 
      ctcacacct gtgggtacc acaatcttc caagacatt 
      ttagccaaa gataaagac gccggcgtc aatggttta 
      gtcgagtat tcgatcgta aaaggcgag aatttagcg 
      cacgaggac gacgatcga atccacagc gaagatggc 
      ttcgggtac tttgccatc aatttacca catcagggt 
      caagttacg gttaataga actctagac tttgagaag 
      acgcagaga tattacgtc accgtcgtg gcgacggac 
      cgggcgcac gatgacaca caacgactt tcgtcaaca 
      acgacactc gtagtcaat attaaagat gacgatgat 
      cagaaccct tctttcata tacaaaggg tgcatgctt 
      ttggatggt acctgcatc aatcctgaa tattcagct 
      tcggtttca agtggggtt cttcacgga atacttaat 
      atttcaccc gaaaaaatc caagcggtg gacatggat 
      acgatcaat tcgcccatt cactactcg tttgtgagt 
      ggtactcct gaatcgtac tctgagtac tttcaaatt 
      gattcggaa acgggtgcc gtgaggcaa atcaaagcc 
      gtggacact tcaaccacc aaacaattc aacatcata 
      atcaaggca caagaaata tccgaagca aaacgttca 
      acaacagcg aaacttttc attactgtt aaaccagtc 
      gatgcaaat cctcctgaa atagaattg tcatcaacc 
      gaaggctat gtcaacgaa aattcccca attggggaa 
      aaagtactc gactctgaa ggggtacca cttctcata 
      tcagtgact gataaggat tttggccca aatgacccc 
      aaaccctct tacacgttc gaactaacg acccctttt 
      ttcacaatt gataaaatg gggtacttg ctagtcaac 
      gaaaacaat ttagaccgt gatcctcca aaccctggc 
      cgttttaaa ttccagatt gttgcacgt gaaaaaggt 
      ggtaccgct gcaagtgcc cccatgtct gtgaccgtt 
      cacttgaac gatgtcaat gataacgcc cccatgttg 
      cctattata gccccagtg ggggttccc gccggggat 
      gtgaaacgc caagtcgcc acaatcaaa gctacagat 
      aatgatgaa ggcgataac gcattagtt acatactca 
      atttatcac gtctcgaat aatggaaat aacaagttc 
      cggattgac cccaatacg ggggtactt gagacggtg 
      gggaaatta aatgcaggg gatcagtac agtttgact 
      gttcaagcc actgataaa ggagggctt tattcacaa 
      gccatcgtt gaagtgacc ataagtcca ggacctaat 
      acacaaagt cccattttt gaacagtcg gtttacgaa 
      attgaggtc agcgaaggg gctacgatt aactcgact 
      gtggccaca attacggcc atggacccc gaaaacgac 
      cctgtgacc tactccata gtctcagga aacgatctg 
      agacaattt tcaattggt gccaaatca ggtgtgatc 
      acagttatc cgaaaattg gatcgtgag gacttgact 
      agatacaaa cttattatt aaggctgaa gatgcgggt 
      aaattatca tcaacagct actgttaat atcaaggtg 
      actgatata aatgacaag aatcccgaa tttgtgggc 
      gatccgtac aatttttcg gtgaaagaa gggctaaat 
      agtacctcg gtagggttt gtaaaggct acggatgcc 
      gatgagggt attaatgcc cttgttacg tactctatt 
      ccctctggt ttgcccttt gatattgat aacgagacc 
      ggcgagata aggactaac cgagccctg gattacgaa 
      acacaaaag aactaccag tttgtggtg acggccaaa 
      gatggcgcc cctgaccca cgtctagcc accgccact 
      gtctccatc tccgtcata gacattgaa gacgaactt 
      ccaattttc acaaaatcg ttatacaca gcgtcagta 
      cccgaaaac attcccgat tatttcatc accgacgtt 
      aaggctgaa gaccccgac actataaaa aaaatcacc 
      tatgtcatc catcaaggt ccaactgac cttttccgt 
      attgacgaa aacagtggt gctgtttat acaatacgt 
      ggtttggac tacgagaaa gacagtcaa catgtcctt 
      accattggt actctcgaa aatatgtcg aatgaggaa 
      ggtagtacc accaaagtg attatcaat gtcgaggac 
      aggaatgat ataccgccg gtttttacc atcataccg 
      cacccaatc actcttgat gatgacgtg gcgattggt 
      actcctgtc actaattta gtggcgacg gactccgat 
      ggtaccgca cccgggaat aaggttcgg tacgagatt 
      ataggacgt ggcaaggcc accaagtac ttccaaatt 
      gactctgat acaggaatt ttaaaagta accaacgat 
      ctcaagaaa gaaattgat acggaatat caggtggac 
      gtccgtgcc tatgattta ggggaacca caattatca 
      tcagttatc actttggat atttacatc caacatgtg 
      gcaacggtc gctcctgaa gttggtttg aaatttgcc 
      gatacgtcg taccatgtg caagtaccc gaaaatgcg 
      acaagtggt accaaagtt aaaacactg accattgtt 
      aatagtcgc acccatggt ggcaatatc ccactcaag 
      tgtctcatt atttcggga aatgagcaa aggaaattc 
      attgtcaat gtgaccgaa gacaggaat tgcgccctt 
      tatttgaat ggtaccatc gattttgaa acagacgag 
      tcgtaccaa cttgaagtc caaatcatg tcactccaa 
      gggttcatt aataaggag ttcagtatc actctagtg 
      accattaat gtggtcgat gttaatgac aatgtccct 
      tatttcatt ttcccctct ggtggtacc cacaagtac 
      cacgccgct atcactgac aattctcct ctttcgaca 
      acagtaacg caaatcaag gctgacgat aaagacagt 
      ggaaagtac ggaaagttg gagttttcg ctcgacaaa 
      accgagtac ttcactata gattcaagt accggcatt 
      gtcaagacc aggaagact tttgacgat gttgataat 
      agtgaatta ccatttaaa ttgattgtc aaaacacgt 
      gataacccg aacgccact gaatacaac gaaatcaaa 
      gcacccttt attgttaat ttgataaca aaagacaat 
      cgcttggta ctagtgatc ggagacgct aaacctgac 
      atagtccag aaaaaacta cccttgatt atcgacata 
      atgcaagaa cagacgggt ttaatagtt ggggtggaa 
      aaactggcc acgagagag tttattggt accaatggt 
      actctcgag actgatcct tcaggtacc gacgtttgg 
      ttctatgtc attgatcca actaccgaa aaaattctc 
      ccacggaat agtactcta gtcaaacgc gcgattttc 
      gacaaacaa gccatggat aacataacg tttgatata 
      acaggccag atccagaac actgccttc cacatacac 
      gaacctttg gtactacaa aaaattaaa agtgcctcc 
      atagcttcc ttcaacggt gaggtgttt ccgtacgct 
      ttgatcatc atcgcgtgc attattttc gtccttgga 
      atcgttggg attatttat atttgcgtg tcttggtcg 
      aggtacaag tcctacaaa gaccaaatg caaagacag 
      tatgtagta ccagcaagt cccacaagg tacgacact 
      gtttatgtt gagccgaac ttgaaagag tacgaaaca 
      caagtgtta caaatgtgc gttcctgtg gacgaacaa 
      gacgactac aacgacctg cacctcgat tttagtaac 
      aagaaccac gccttcagt cttgacaat gtaagttat 
      atcagtaag gacaataat ttgaagaag tacgggggg 
      caaagtccg gtcagtagc gagtctgcg accactgtg 
      agggcgtcc agtgtggga gaagggcac gtgttgaag 
      tataggtcg gacgatgac gaaatgaac gccagtccg 
      agtaatgac aatgtgatg tttaaagag aagaaggac 
      tactcgcat ttggggttc tcgtacgac cattcgccg 
      gttgagacg accaccgag ttgtag
    mRNA sequence
    • >TC011374 RA
      taggtagta cgacaggtg cggcactgc attctcaac 
      cattatcgt accatcgcg tttcaaaaa tcgcaattt 
      atgtgacaa tgtgtgaaa aatttgtat ttagtgcca 
      aaaaaagtg ctcataagt gacccaata atatatgga 
      tacgtcgtt gctgaccca tcgtccgac catcaaaga 
      tgacgaccc aacgcctga tttttgcac tcctggctt 
      gttgcagcg ttcggatgg tacttgcag ggttgtgtg 
      aagtggact cgggtcaat cgaacataa tcctggaca 
      ttgaagaaa gtcgcggaa aatcaatta gccaagaga 
      ccgttcctg cagagttac caattgttg gcgagccaa 
      atgtcgata ttttcctcg atttggcgt ttcccaaag 
      ggcctgcgc ttttttttc tcaatggga aaaagctgc 
      aattgttgg cgccgttag atcgagacg aaaacagtc 
      tctcgcata ttgtttttc agttaattt gcacaatta 
      aatcgactc acaagaaac gaactgtac cagtaattg 
      taagactga gtgatatta acgataatg caccagaat 
      tcgtcaaca cgccgtacg agacgagta tatcggagc 
      tcacacctg tgggtacca caatcttcc aagacattt 
      tagccaaag ataaagacg ccggcgtca atggtttag 
      tcgagtatt cgatcgtaa aaggcgaga atttagcgc 
      acgaggacg acgatcgaa tccacagcg aagatggct 
      tcgggtact ttgccatca atttaccac atcagggtc 
      aagttacgg ttaatagaa ctctagact ttgagaaga 
      cgcagagat attacgtca ccgtcgtgg cgacggacc 
      gggcgcacg atgacacac aacgacttt cgtcaacaa 
      cgacactcg tagtcaata ttaaagatg acgatgatc 
      agaaccctt ctttcatat acaaagggt gcatgcttt 
      tggatggta cctgcatca atcctgaat attcagctt 
      cggtttcaa gtggggttc ttcacggaa tacttaata 
      tttcacccg aaaaaatcc aagcggtgg acatggata 
      cgatcaatt cgcccattc actactcgt ttgtgagtg 
      gtactcctg aatcgtact ctgagtact ttcaaattg 
      attcggaaa cgggtgccg tgaggcaaa tcaaagccg 
      tggacactt caaccacca aacaattca acatcataa 
      tcaaggcac aagaaatat ccgaagcaa aacgttcaa 
      caacagcga aacttttca ttactgtta aaccagtcg 
      atgcaaatc ctcctgaaa tagaattgt catcaaccg 
      aaggctatg tcaacgaaa attccccaa ttggggaaa 
      aagtactcg actctgaag gggtaccac ttctcatat 
      cagtgactg ataaggatt ttggcccaa atgacccca 
      aaccctctt acacgttcg aactaacga ccccttttt 
      tcacaattg ataaaatgg ggtacttgc tagtcaacg 
      aaaacaatt tagaccgtg atcctccaa accctggcc 
      gttttaaat tccagattg ttgcacgtg aaaaaggtg 
      gtaccgctg caagtgccc ccatgtctg tgaccgttc 
      acttgaacg atgtcaatg ataacgccc ccatgttgc 
      ctattatag ccccagtgg gggttcccg ccggggatg 
      tgaaacgcc aagtcgcca caatcaaag ctacagata 
      atgatgaag gcgataacg cattagtta catactcaa 
      tttatcacg tctcgaata atggaaata acaagttcc 
      ggattgacc ccaatacgg gggtacttg agacggtgg 
      ggaaattaa atgcagggg atcagtaca gtttgactg 
      ttcaagcca ctgataaag gagggcttt attcacaag 
      ccatcgttg aagtgacca taagtccag gacctaata 
      cacaaagtc ccatttttg aacagtcgg tttacgaaa 
      ttgaggtca gcgaagggg ctacgatta actcgactg 
      tggccacaa ttacggcca tggaccccg aaaacgacc 
      ctgtgacct actccatag tctcaggaa acgatctga 
      gacaatttt caattggtg ccaaatcag gtgtgatca 
      cagttatcc gaaaattgg atcgtgagg acttgacta 
      gatacaaac ttattatta aggctgaag atgcgggta 
      aattatcat caacagcta ctgttaata tcaaggtga 
      ctgatataa atgacaaga atcccgaat ttgtgggcg 
      atccgtaca atttttcgg tgaaagaag ggctaaata 
      gtacctcgg tagggtttg taaaggcta cggatgccg 
      atgagggta ttaatgccc ttgttacgt actctattc 
      cctctggtt tgccctttg atattgata acgagaccg 
      gcgagataa ggactaacc gagccctgg attacgaaa 
      cacaaaaga actaccagt ttgtggtga cggccaaag 
      atggcgccc ctgacccac gtctagcca ccgccactg 
      tctccatct ccgtcatag acattgaag acgaacttc 
      caattttca caaaatcgt tatacacag cgtcagtac 
      ccgaaaaca ttcccgatt atttcatca ccgacgtta 
      aggctgaag accccgaca ctataaaaa aaatcacct 
      atgtcatcc atcaaggtc caactgacc ttttccgta 
      ttgacgaaa acagtggtg ctgtttata caatacgtg 
      gtttggact acgagaaag acagtcaac atgtcctta 
      ccattggta ctctcgaaa atatgtcga atgaggaag 
      gtagtacca ccaaagtga ttatcaatg tcgaggaca 
      ggaatgata taccgccgg tttttacca tcataccgc 
      acccaatca ctcttgatg atgacgtgg cgattggta 
      ctcctgtca ctaatttag tggcgacgg actccgatg 
      gtaccgcac ccgggaata aggttcggt acgagatta 
      taggacgtg gcaaggcca ccaagtact tccaaattg 
      actctgata caggaattt taaaagtaa ccaacgatc 
      tcaagaaag aaattgata cggaatatc aggtggacg 
      tccgtgcct atgatttag gggaaccac aattatcat 
      cagttatca ctttggata tttacatcc aacatgtgg 
      caacggtcg ctcctgaag ttggtttga aatttgccg 
      atacgtcgt accatgtgc aagtacccg aaaatgcga 
      caagtggta ccaaagtta aaacactga ccattgtta 
      atagtcgca cccatggtg gcaatatcc cactcaagt 
      gtctcatta tttcgggaa atgagcaaa ggaaattca 
      ttgtcaatg tgaccgaag acaggaatt gcgcccttt 
      atttgaatg gtaccatcg attttgaaa cagacgagt 
      cgtaccaac ttgaagtcc aaatcatgt cactccaag 
      ggttcatta ataaggagt tcagtatca ctctagtga 
      ccattaatg tggtcgatg ttaatgaca atgtccctt 
      atttcattt tcccctctg gtggtaccc acaagtacc 
      acgccgcta tcactgaca attctcctc tttcgacaa 
      cagtaacgc aaatcaagg ctgacgata aagacagtg 
      gaaagtacg gaaagttgg agttttcgc tcgacaaaa 
      ccgagtact tcactatag attcaagta ccggcattg 
      tcaagacca ggaagactt ttgacgatg ttgataata 
      gtgaattac catttaaat tgattgtca aaacacgtg 
      ataacccga acgccactg aatacaacg aaatcaaag 
      cacccttta ttgttaatt tgataacaa aagacaatc 
      gcttggtac tagtgatcg gagacgcta aacctgaca 
      tagtccaga aaaaactac ccttgatta tcgacataa 
      tgcaagaac agacgggtt taatagttg gggtggaaa 
      aactggcca cgagagagt ttattggta ccaatggta 
      ctctcgaga ctgatcctt caggtaccg acgtttggt 
      tctatgtca ttgatccaa ctaccgaaa aaattctcc 
      cacggaata gtactctag tcaaacgcg cgattttcg 
      acaaacaag ccatggata acataacgt ttgatataa 
      caggccaga tccagaaca ctgccttcc acatacacg 
      aacctttgg tactacaaa aaattaaaa gtgcctcca 
      tagcttcct tcaacggtg aggtgtttc cgtacgctt 
      tgatcatca tcgcgtgca ttattttcg tccttggaa 
      tcgttggga ttatttata tttgcgtgt cttggtcga 
      ggtacaagt cctacaaag accaaatgc aaagacagt 
      atgtagtac cagcaagtc ccacaaggt acgacactg 
      tttatgttg agccgaact tgaaagagt acgaaacac 
      aagtgttac aaatgtgcg ttcctgtgg acgaacaag 
      acgactaca acgacctgc acctcgatt ttagtaaca 
      agaaccacg ccttcagtc ttgacaatg taagttata 
      tcagtaagg acaataatt tgaagaagt acggggggc 
      aaagtccgg tcagtagcg agtctgcga ccactgtga 
      gggcgtcca gtgtgggag aagggcacg tgttgaagt 
      ataggtcgg acgatgacg aaatgaacg ccagtccga 
      gtaatgaca atgtgatgt ttaaagaga agaaggact 
      actcgcatt tggggttct cgtacgacc attcgccgg 
      ttgagacga ccaccgagt tgtagataa atgtaaatg 
      gaataactg attaattta atgtaatta tgaggttct 
      aggaatgta atctgattg taattttat attttaata 
      aatgttttt tatttaa