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Gene information
General information
TC031391
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011371
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid105: 267240..273304
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031391
    iBeetle screen iB_02633
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Adult morphology
    elytra shape irregular - Number of animals showing the phenotype: 5

    elytra symmetry decreased - Number of animals showing the phenotype: 5


    abdominal odoriferous glands shape irregular - Number of animals showing the phenotype: 4 (41 dpi)
    Days post injection



    abdominal odoriferous glands partially content not present - Number of animals showing the phenotype: 3 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031391 RA
      MQVILYHNQ SGEAAFSGD LKQKEVHRA ITLHPVKQP 
      QHMYRLDKY VKGLKIQAY QQECIDLHR GIASSMSQM 
      GYTIGRLPN ASLVQGLPL FAAPRGSQL YLGDPTLLG 
      MPQSLTRHQ PRAVQEVLD WELISKTLY SYKDLNPRR 
      RIGSALKEG LNDVVRDIM EVINSYSKQ RGRVIDFKE 
      ILYGYWRLD PVHGIDLIL DLLLVYRKY RGHKMTVQV 
      RRHAYVQQT FTGIFVREI TKASPSSVA VPKIVKHII 
      STFEKIPPI FPSITAKNQ RQTVNFIVP LSGRFDTFR 
      RFLRLYEDI CIKGGEETR LFVILYQSV GFEASARAI 
      EGLQGRYLN SGVSVVYVN ESFSRAKAL ENGLKFVSD 
      DDLVLFIDV DMVFDARSL QRVRQNTVK GRSVYFPIV 
      YSLFDPRIT NRSLGIDEE SGFWRQFGF GIVSIFKND 
      YKKLGGFNL NISGWGFED VAFYDTAIQ SDLRIVRSA 
      DPGLVHVYH PIHCDINLD SNQRNMCLG TQANMLGSR 
      KQLEAIFRR FRHLFS
    CDS sequence
    • >TC031391 RA
      atgcaagtc attttatat cacaaccaa agcggtgaa 
      gctgcattc agtggcgat cttaaacag aaggaagtc 
      caccgtgcg atcacttta cacccggtc aagcaacct 
      caacacatg tataggctc gataaatac gtgaagggt 
      ttgaaaatc caggcgtac caacaggag tgtatcgac 
      ctccaccga ggcatcgcc tcgtccatg tcccagatg 
      ggttatacc attgggcgt ttgcccaac gccagtcta 
      gtccaaggt ttgccactt tttgccgca ccaagaggc 
      tcacaactc tatttgggc gaccctaca ctcttgggc 
      atgcctcag agcctcacg aggcaccag ccgagggct 
      gtgcaagaa gttctcgac tgggagctc atctccaaa 
      acactatat tcttacaag gatttgaat ccaaggcgc 
      cggattggc tccgccctc aaggagggc ctaaatgac 
      gtagttcgt gacattatg gaagttatc aactcgtat 
      tcgaagcaa cgaggccgg gttatagat tttaaggag 
      attttgtat ggttattgg aggttggac cccgtacat 
      gggattgat ttaattttg gaccttttg ctcgtttat 
      cgcaagtac agagggcac aagatgaca gtgcaagtg 
      cgaaggcat gcttacgtg cagcaaact tttacaggt 
      atattcgtt cgtgaaatc accaaagca tcgccttct 
      agtgtcgcc gtccccaaa atcgtcaaa cacatcatt 
      tcgacgttt gagaaaatt ccgcctatt tttccgtca 
      atcaccgcc aaaaatcaa cgtcaaact gtcaatttt 
      attgtgcct ttgagcggc cgttttgac acatttcgg 
      cgctttctg cgattatat gaagacatt tgtatcaaa 
      ggtggcgaa gaaacgcgc ttatttgta attttgtat 
      caaagtgtg ggctttgag gcgagtgct agggctata 
      gaagggcta caagggcgg tatttgaac agtggcgtg 
      agtgtggtg tacgttaac gaaagtttt agtcgggct 
      aaagcccta gaaaatggg ctaaagttt gtgagtgat 
      gacgattta gtgctattt attgacgtt gatatggtt 
      tttgatgca cgaagtttg cagcgagtg cgtcaaaat 
      accgtcaag ggccgaagt gtttatttt ccgatagtt 
      tacagtttg tttgaccct agaattacg aatcgaagt 
      ttaggcatc gatgaagag tcgggtttt tggcgccag 
      tttggtttc ggtatagtt agcatcttt aaaaatgac 
      tacaaaaaa ttgggcggg tttaatttg aacatttcg 
      gggtggggc ttcgaagat gtggcgttt tacgacacc 
      gctatccaa tcagatttg aggattgtg cgcagtgct 
      gatcccgga ttggtccac gtgtatcac ccgattcat 
      tgtgatatt aatttagac agcaatcag cggaatatg 
      tgtttaggg acgcaggcg aatatgtta ggaagtcgc 
      aagcaattg gaggcgatt tttcgacga tttagacat 
      ttgtttagt tga
    mRNA sequence
    • >TC031391 RA
      acgctgaaa acagcatct tattcgtaa aattttaaa 
      ataaacgat taaagttca ttgcattta ttgcaacga 
      caaaacgct catatttta tcacactct tgtataaat 
      attggggca aaagtcaaa aatcatcat ttttattga 
      cacatattc ctatttctg gcacgtttt aattcctta 
      tctctggtt atctgatat ccgagttat tcaaactat 
      gcacacgac taacataaa atcgcattt gcgagatat 
      ttgacccaa ttttataaa aaaaaaaag cgaatcaat 
      caagatggc ttgattgaa caattgcta ccacagata 
      agttgataa gcacacacg cgtgatttt ttccaattg 
      ataagaacc tctcattca gcatccaat caccagatg 
      atttatctg attttttga tatgagttt gacagcgga 
      tgggcttag gtagaattt ttgctattg ttagtcggc 
      aacaggcaa cgttcaagt tcaaggcca gcccggatg 
      tggcatctg gagaccatc tttgtgttc ggctctcga 
      gtttttgtc tcgagccgt tgaattctc ttaatttta 
      ttgaaaaaa gtcggggga aaaattcca ctataggcc 
      cgccaacgc ttgttaagc ccaatgttc gaattgaaa 
      tataatgac ttagaccct gaagtcatt caatattat 
      taaaattaa cgttctcta atagatgca agtcatttt 
      atatcacaa ccaaagcgg tgaagctgc attcagtgg 
      cgatcttaa acagaagga agtccaccg tgcgatcac 
      tttacaccc ggtcaagca acctcaaca catgtatag 
      gctcgataa atacgtgaa gggtttgaa aatccaggc 
      gtaccaaca ggagtgtat cgacctcca ccgaggcat 
      cgcctcgtc catgtccca gatgggtta taccattgg 
      gcgtttgcc caacgccag tctagtcca aggtttgcc 
      actttttgc cgcaccaag aggctcaca actctattt 
      gggcgaccc tacactctt gggcatgcc tcagagcct 
      cacgaggca ccagccgag ggctgtgca agaagttct 
      cgactggga gctcatctc caaaacact atattctta 
      caaggattt gaatccaag gcgccggat tggctccgc 
      cctcaagga gggcctaaa tgacgtagt tcgtgacat 
      tatggaagt tatcaactc gtattcgaa gcaacgagg 
      ccgggttat agattttaa ggagatttt gtatggtta 
      ttggaggtt ggaccccgt acatgggat tgatttaat 
      tttggacct tttgctcgt ttatcgcaa gtacagagg 
      gcacaagat gacagtgca agtgcgaag gcatgctta 
      cgtgcagca aacttttac aggtatatt cgttcgtga 
      aatcaccaa agcatcgcc ttctagtgt cgccgtccc 
      caaaatcgt caaacacat catttcgac gtttgagaa 
      aattccgcc tatttttcc gtcaatcac cgccaaaaa 
      tcaacgtca aactgtcaa ttttattgt gcctttgag 
      cggccgttt tgacacatt tcggcgctt tctgcgatt 
      atatgaaga catttgtat caaaggtgg cgaagaaac 
      gcgcttatt tgtaatttt gtatcaaag tgtgggctt 
      tgaggcgag tgctagggc tatagaagg gctacaagg 
      gcggtattt gaacagtgg cgtgagtgt ggtgtacgt 
      taacgaaag ttttagtcg ggctaaagc cctagaaaa 
      tgggctaaa gtttgtgag tgatgacga tttagtgct 
      atttattga cgttgatat ggtttttga tgcacgaag 
      tttgcagcg agtgcgtca aaataccgt caagggccg 
      aagtgttta ttttccgat agtttacag tttgtttga 
      ccctagaat tacgaatcg aagtttagg catcgatga 
      agagtcggg tttttggcg ccagtttgg tttcggtat 
      agttagcat ctttaaaaa tgactacaa aaaattggg 
      cgggtttaa tttgaacat ttcggggtg gggcttcga 
      agatgtggc gttttacga caccgctat ccaatcaga 
      tttgaggat tgtgcgcag tgctgatcc cggattggt 
      ccacgtgta tcacccgat tcattgtga tattaattt 
      agacagcaa tcagcggaa tatgtgttt agggacgca 
      ggcgaatat gttaggaag tcgcaagca attggaggc 
      gatttttcg acgatttag acatttgtt tagttgact 
      gtgattgta aagggtgat tatacgttt tttgtatgt 
      tcctatagc gagataatc tcacggtta catgacaag 
      gctgaggcg agttgagca aaattgtat ttatttttt 
      attgagatt ttaaatgcc aaaatgtac tgccatatg 
      tgtgaaaga caagtatga aataaaaat tttatgttt 
      ac