General information | |||
TC031391
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015450233.1: 267239..273303 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
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TC031391 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_02633 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
elytra symmetry decreased - Number of animals showing the phenotype: 5
abdominal odoriferous glands shape irregular - Number of animals showing the phenotype: 4 (41 dpi)
abdominal odoriferous glands partially content not present - Number of animals showing the phenotype: 3 (41 dpi)
- >TC031391 RA
MQVILYHNQ SGEAAFSGD LKQKEVHRA ITLHPVKQP QHMYRLDKY VKGLKIQAY QQECIDLHR GIASSMSQM GYTIGRLPN ASLVQGLPL FAAPRGSQL YLGDPTLLG MPQSLTRHQ PRAVQEVLD WELISKTLY SYKDLNPRR RIGSALKEG LNDVVRDIM EVINSYSKQ RGRVIDFKE ILYGYWRLD PVHGIDLIL DLLLVYRKY RGHKMTVQV RRHAYVQQT FTGIFVREI TKASPSSVA VPKIVKHII STFEKIPPI FPSITAKNQ RQTVNFIVP LSGRFDTFR RFLRLYEDI CIKGGEETR LFVILYQSV GFEASARAI EGLQGRYLN SGVSVVYVN ESFSRAKAL ENGLKFVSD DDLVLFIDV DMVFDARSL QRVRQNTVK GRSVYFPIV YSLFDPRIT NRSLGIDEE SGFWRQFGF GIVSIFKND YKKLGGFNL NISGWGFED VAFYDTAIQ SDLRIVRSA DPGLVHVYH PIHCDINLD SNQRNMCLG TQANMLGSR KQLEAIFRR FRHLFS
- >TC031391 RA
atgcaagtc attttatat cacaaccaa agcggtgaa gctgcattc agtggcgat cttaaacag aaggaagtc caccgtgcg atcacttta cacccggtc aagcaacct caacacatg tataggctc gataaatac gtgaagggt ttgaaaatc caggcgtac caacaggag tgtatcgac ctccaccga ggcatcgcc tcgtccatg tcccagatg ggttatacc attgggcgt ttgcccaac gccagtcta gtccaaggt ttgccactt tttgccgca ccaagaggc tcacaactc tatttgggc gaccctaca ctcttgggc atgcctcag agcctcacg aggcaccag ccgagggct gtgcaagaa gttctcgac tgggagctc atctccaaa acactatat tcttacaag gatttgaat ccaaggcgc cggattggc tccgccctc aaggagggc ctaaatgac gtagttcgt gacattatg gaagttatc aactcgtat tcgaagcaa cgaggccgg gttatagat tttaaggag attttgtat ggttattgg aggttggac cccgtacat gggattgat ttaattttg gaccttttg ctcgtttat cgcaagtac agagggcac aagatgaca gtgcaagtg cgaaggcat gcttacgtg cagcaaact tttacaggt atattcgtt cgtgaaatc accaaagca tcgccttct agtgtcgcc gtccccaaa atcgtcaaa cacatcatt tcgacgttt gagaaaatt ccgcctatt tttccgtca atcaccgcc aaaaatcaa cgtcaaact gtcaatttt attgtgcct ttgagcggc cgttttgac acatttcgg cgctttctg cgattatat gaagacatt tgtatcaaa ggtggcgaa gaaacgcgc ttatttgta attttgtat caaagtgtg ggctttgag gcgagtgct agggctata gaagggcta caagggcgg tatttgaac agtggcgtg agtgtggtg tacgttaac gaaagtttt agtcgggct aaagcccta gaaaatggg ctaaagttt gtgagtgat gacgattta gtgctattt attgacgtt gatatggtt tttgatgca cgaagtttg cagcgagtg cgtcaaaat accgtcaag ggccgaagt gtttatttt ccgatagtt tacagtttg tttgaccct agaattacg aatcgaagt ttaggcatc gatgaagag tcgggtttt tggcgccag tttggtttc ggtatagtt agcatcttt aaaaatgac tacaaaaaa ttgggcggg tttaatttg aacatttcg gggtggggc ttcgaagat gtggcgttt tacgacacc gctatccaa tcagatttg aggattgtg cgcagtgct gatcccgga ttggtccac gtgtatcac ccgattcat tgtgatatt aatttagac agcaatcag cggaatatg tgtttaggg acgcaggcg aatatgtta ggaagtcgc aagcaattg gaggcgatt tttcgacga tttagacat ttgtttagt tga
- >TC031391 RA
acgctgaaa acagcatct tattcgtaa aattttaaa ataaacgat taaagttca ttgcattta ttgcaacga caaaacgct catatttta tcacactct tgtataaat attggggca aaagtcaaa aatcatcat ttttattga cacatattc ctatttctg gcacgtttt aattcctta tctctggtt atctgatat ccgagttat tcaaactat gcacacgac taacataaa atcgcattt gcgagatat ttgacccaa ttttataaa aaaaaaaag cgaatcaat caagatggc ttgattgaa caattgcta ccacagata agttgataa gcacacacg cgtgatttt ttccaattg ataagaacc tctcattca gcatccaat caccagatg atttatctg attttttga tatgagttt gacagcgga tgggcttag gtagaattt ttgctattg ttagtcggc aacaggcaa cgttcaagt tcaaggcca gcccggatg tggcatctg gagaccatc tttgtgttc ggctctcga gtttttgtc tcgagccgt tgaattctc ttaatttta ttgaaaaaa gtcggggga aaaattcca ctataggcc cgccaacgc ttgttaagc ccaatgttc gaattgaaa tataatgac ttagaccct gaagtcatt caatattat taaaattaa cgttctcta atagatgca agtcatttt atatcacaa ccaaagcgg tgaagctgc attcagtgg cgatcttaa acagaagga agtccaccg tgcgatcac tttacaccc ggtcaagca acctcaaca catgtatag gctcgataa atacgtgaa gggtttgaa aatccaggc gtaccaaca ggagtgtat cgacctcca ccgaggcat cgcctcgtc catgtccca gatgggtta taccattgg gcgtttgcc caacgccag tctagtcca aggtttgcc actttttgc cgcaccaag aggctcaca actctattt gggcgaccc tacactctt gggcatgcc tcagagcct cacgaggca ccagccgag ggctgtgca agaagttct cgactggga gctcatctc caaaacact atattctta caaggattt gaatccaag gcgccggat tggctccgc cctcaagga gggcctaaa tgacgtagt tcgtgacat tatggaagt tatcaactc gtattcgaa gcaacgagg ccgggttat agattttaa ggagatttt gtatggtta ttggaggtt ggaccccgt acatgggat tgatttaat tttggacct tttgctcgt ttatcgcaa gtacagagg gcacaagat gacagtgca agtgcgaag gcatgctta cgtgcagca aacttttac aggtatatt cgttcgtga aatcaccaa agcatcgcc ttctagtgt cgccgtccc caaaatcgt caaacacat catttcgac gtttgagaa aattccgcc tatttttcc gtcaatcac cgccaaaaa tcaacgtca aactgtcaa ttttattgt gcctttgag cggccgttt tgacacatt tcggcgctt tctgcgatt atatgaaga catttgtat caaaggtgg cgaagaaac gcgcttatt tgtaatttt gtatcaaag tgtgggctt tgaggcgag tgctagggc tatagaagg gctacaagg gcggtattt gaacagtgg cgtgagtgt ggtgtacgt taacgaaag ttttagtcg ggctaaagc cctagaaaa tgggctaaa gtttgtgag tgatgacga tttagtgct atttattga cgttgatat ggtttttga tgcacgaag tttgcagcg agtgcgtca aaataccgt caagggccg aagtgttta ttttccgat agtttacag tttgtttga ccctagaat tacgaatcg aagtttagg catcgatga agagtcggg tttttggcg ccagtttgg tttcggtat agttagcat ctttaaaaa tgactacaa aaaattggg cgggtttaa tttgaacat ttcggggtg gggcttcga agatgtggc gttttacga caccgctat ccaatcaga tttgaggat tgtgcgcag tgctgatcc cggattggt ccacgtgta tcacccgat tcattgtga tattaattt agacagcaa tcagcggaa tatgtgttt agggacgca ggcgaatat gttaggaag tcgcaagca attggaggc gatttttcg acgatttag acatttgtt tagttgact gtgattgta aagggtgat tatacgttt tttgtatgt tcctatagc gagataatc tcacggtta catgacaag gctgaggcg agttgagca aaattgtat ttatttttt attgagatt ttaaatgcc aaaatgtac tgccatatg tgtgaaaga caagtatga aataaaaat tttatgttt ac
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
