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Gene information
General information
TC031367
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011323
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid74: 1018948..1026697
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031367
    iBeetle screen iB_02631
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature sometimes partiallynot present - Penetrance (percentage of animals/eggs on slide): more than 80%

    larval musculature sometimes partiallythinner - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection



    pupal molt not completely molted - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection



    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Pupal morphology
    wings shortened - Number of animals showing the phenotype: 3

    legs orientation irregular - Number of animals showing the phenotype: 3
    wings partially potentiallynecrotic - Number of animals showing the phenotype: 3
    urogomphi orientation irregular - Number of animals showing the phenotype: 3

    female genital lobes orientation irregular - Number of animals showing the phenotype: 3
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031367 RA
      MQQELDSDY ESEYYYSPV KKPANMDDI LIVSFKGSE 
      AADLWAEYF TNYFQQLSK ANKKPFKIQ HMLLEDCIS 
      EPKEDPKII AERATNVKL QLVILCPNF LDFVAEYPD 
      KCSELGKLL LADRTLALL LGVSDSDLT EVHKKTFPT 
      YFQWQRLGV GQDQDENFT KEFLGHAMS ILSRIWKQQ 
      TSVTQEKSY FSVSPKKIR QGQNSVFIL LTYPLQKED 
      ILKVSIERN NEIFEVKTV KRRNPYTIK ISVPNNLTE 
      VTAIVNILV EKNGSIVGS RPIKCESRL RELEQILRL 
      TNNPIEFMC QTLGFNPSS REQLDNWLL QAFQRNLPA 
      HFNLLANHD TPFGASVQL HKHSHEEHP TLLHFAAKF 
      GFEKLALQL LDCPGADVA FDIKNIHDM TPSEIAETN 
      GHPELAATL RGYMNMNEF THMYSKLKE MSLNSAKID 
      EDSYITPKE IEELYKICP APRPVFFGD SLNSPSSEC 
      GYMSMTPPV KIIIEHSEP AKSPTPSIK TDAPSSLPP 
      NEITEDKVQ KELLEIIND FKNNVHSIS QVEKLVEEW 
      KNRNDVQKS FKEKQEQLK EMRLKYEKI QQEMKASMK 
      KPTPFERVR KLFGRSKSK EEKHEISSP VLASPSIVA 
      IQTQRPISS LSTSSSGSS GRMSTISGC SLGDSGTHS 
      DNEERKNML GSHNEEDFR NVFNKAALE LNYTPVPAP 
      KPVKTGIFS NRNQLFETI EEKPIARPD SLPVGEEYY 
      IQFPRSGLP VAGVEEKAG QEYMNVGAA KDEETHEYM 
      NFKVMTKR
    CDS sequence
    • >TC031367 RA
      atgcagcaa gagctagac tccgactat gagtcggaa 
      tactattat tcccctgtc aaaaagccg gctaacatg 
      gacgacatc ctcatagtg tccttcaaa ggcagcgaa 
      gctgcggat ctctgggcc gagtatttc accaactac 
      tttcaacag ttgagcaaa gcgaacaaa aaacccttc 
      aagattcag catatgcta ctagaagac tgtatttcc 
      gagccgaag gaagacccg aaaatcatc gccgaacgc 
      gcgaccaac gtcaagtta caactagtt atactttgt 
      ccgaatttt ctggatttt gtggccgaa tatcccgac 
      aagtgctcc gagttaggg aagttgctc ctcgccgat 
      cggactcta gccctactc ttgggagtc tccgacagc 
      gacttgacc gaagtccac aagaaaact ttcccgacc 
      tacttccag tggcaaaga ctgggcgtg ggccaggac 
      caagacgag aacttcacc aaggagttc ctcggccac 
      gccatgtcc atcctatcc cgaatctgg aaacagcaa 
      acttcggta acccaggag aagtcgtat ttttccgtc 
      tctccgaaa aaaatccgc caaggccag aacagcgtg 
      ttcatcctc ctgacctac cccttgcaa aaggaggac 
      attttgaag gtttccatc gaacgcaac aacgaaatc 
      ttcgaagtg aagaccgta aaacggcgc aacccctac 
      acaatcaaa atctccgtt cccaacaac ttgaccgaa 
      gtcacagcc atagtgaac atccttgtt gaaaaaaac 
      ggcagcatc gtgggcagt cgtccaatc aagtgcgag 
      agcagactc agagagctt gaacaaatt ttgcgcctc 
      actaataat ccgatcgag tttatgtgt cagacgttg 
      ggctttaac ccgtccagt cgcgagcag ttggataat 
      tggctgctc caggccttc cagaggaac ctgccagcg 
      catttcaac ctcttggcc aatcacgac acgccattt 
      ggcgcctcc gtacagctc cacaagcac agtcacgag 
      gagcacccc actctgctc catttcgcc gccaaattc 
      ggattcgaa aaactcgcc ctgcaactg ctggactgt 
      ccgggggct gatgtcgcg ttcgatatc aaaaatata 
      cacgatatg acgccttct gagattgct gagaccaat 
      ggacacccg gaacttgcg gccacactg cgtgggtac 
      atgaatatg aacgagttc acgcacatg tactccaaa 
      ttgaaggaa atgagcttg aattcggct aaaatagac 
      gaggacagt tatatcacc ccgaaagag atcgaagaa 
      ctgtacaaa atatgcccc gcccctaga cccgtcttc 
      ttcggcgac tctttaaac tcaccgagc agtgagtgt 
      ggctacatg tccatgact ccgcccgtc aaaataatc 
      atcgaacac tccgaacca gccaaaagc cccacccct 
      tcaatcaag actgacgcc ccttcttca ctcccaccc 
      aacgaaata actgaagac aaagtccaa aaggagctc 
      ctcgaaatc atcaacgat ttcaaaaac aacgtccac 
      tcaatttcc caagttgaa aaactagtc gaggagtgg 
      aaaaaccgc aacgatgta caaaaatcc ttcaaagag 
      aagcaagaa cagttgaag gaaatgcgg ttaaagtac 
      gagaaaata cagcaagag atgaaagct tcaatgaaa 
      aagcccact cccttcgaa agggtgaga aagttgttc 
      gggagaagc aaatcaaag gaagaaaaa cacgaaatt 
      tcatcaccg gtgttggcg tcgcccagt attgtggcc 
      attcagacc caaagaccc atcagcagt ctgagtacc 
      tcaagttct ggttcctcg ggccggatg agtaccatc 
      agtgggtgc agtttgggc gacagtggc acccattcc 
      gacaacgag gaacgaaaa aatatgctg gggagtcac 
      aatgaagaa gatttccga aatgtgttc aataaggct 
      gccttagag ttaaattac acgccagtt ccggcccct 
      aaaccggtc aaaaccggg attttctcc aatcggaat 
      cagttattt gaaaccatt gaggagaag ccaatcgca 
      aggcccgac tcacttccg gtaggggag gagtattac 
      atacagttt ccgcgatcg ggcttacca gtggcagga 
      gttgaggag aaagccggc caagagtac atgaacgtg 
      ggggctgcc aaagacgaa gagacccac gaatacatg 
      aacttcaaa gtaatgacc aagagatag
    mRNA sequence
    • >TC031367 RA
      cttgtaaca aactagtcg cattcaagc tgcgttgag 
      tattgtgtg tctggctgt agaggggtt gtagagggt 
      gctaggagt ggctgaaca atgcagcaa gagctagac 
      tccgactat gagtcggaa tactattat tcccctgtc 
      aaaaagccg gctaacatg gacgacatc ctcatagtg 
      tccttcaaa ggcagcgaa gctgcggat ctctgggcc 
      gagtatttc accaactac tttcaacag ttgagcaaa 
      gcgaacaaa aaacccttc aagattcag catatgcta 
      ctagaagac tgtatttcc gagccgaag gaagacccg 
      aaaatcatc gccgaacgc gcgaccaac gtcaagtta 
      caactagtt atactttgt ccgaatttt ctggatttt 
      gtggccgaa tatcccgac aagtgctcc gagttaggg 
      aagttgctc ctcgccgat cggactcta gccctactc 
      ttgggagtc tccgacagc gacttgacc gaagtccac 
      aagaaaact ttcccgacc tacttccag tggcaaaga 
      ctgggcgtg ggccaggac caagacgag aacttcacc 
      aaggagttc ctcggccac gccatgtcc atcctatcc 
      cgaatctgg aaacagcaa acttcggta acccaggag 
      aagtcgtat ttttccgtc tctccgaaa aaaatccgc 
      caaggccag aacagcgtg ttcatcctc ctgacctac 
      cccttgcaa aaggaggac attttgaag gtttccatc 
      gaacgcaac aacgaaatc ttcgaagtg aagaccgta 
      aaacggcgc aacccctac acaatcaaa atctccgtt 
      cccaacaac ttgaccgaa gtcacagcc atagtgaac 
      atccttgtt gaaaaaaac ggcagcatc gtgggcagt 
      cgtccaatc aagtgcgag agcagactc agagagctt 
      gaacaaatt ttgcgcctc actaataat ccgatcgag 
      tttatgtgt cagacgttg ggctttaac ccgtccagt 
      cgcgagcag ttggataat tggctgctc caggccttc 
      cagaggaac ctgccagcg catttcaac ctcttggcc 
      aatcacgac acgccattt ggcgcctcc gtacagctc 
      cacaagcac agtcacgag gagcacccc actctgctc 
      catttcgcc gccaaattc ggattcgaa aaactcgcc 
      ctgcaactg ctggactgt ccgggggct gatgtcgcg 
      ttcgatatc aaaaatata cacgatatg acgccttct 
      gagattgct gagaccaat ggacacccg gaacttgcg 
      gccacactg cgtgggtac atgaatatg aacgagttc 
      acgcacatg tactccaaa ttgaaggaa atgagcttg 
      aattcggct aaaatagac gaggacagt tatatcacc 
      ccgaaagag atcgaagaa ctgtacaaa atatgcccc 
      gcccctaga cccgtcttc ttcggcgac tctttaaac 
      tcaccgagc agtgagtgt ggctacatg tccatgact 
      ccgcccgtc aaaataatc atcgaacac tccgaacca 
      gccaaaagc cccacccct tcaatcaag actgacgcc 
      ccttcttca ctcccaccc aacgaaata actgaagac 
      aaagtccaa aaggagctc ctcgaaatc atcaacgat 
      ttcaaaaac aacgtccac tcaatttcc caagttgaa 
      aaactagtc gaggagtgg aaaaaccgc aacgatgta 
      caaaaatcc ttcaaagag aagcaagaa cagttgaag 
      gaaatgcgg ttaaagtac gagaaaata cagcaagag 
      atgaaagct tcaatgaaa aagcccact cccttcgaa 
      agggtgaga aagttgttc gggagaagc aaatcaaag 
      gaagaaaaa cacgaaatt tcatcaccg gtgttggcg 
      tcgcccagt attgtggcc attcagacc caaagaccc 
      atcagcagt ctgagtacc tcaagttct ggttcctcg 
      ggccggatg agtaccatc agtgggtgc agtttgggc 
      gacagtggc acccattcc gacaacgag gaacgaaaa 
      aatatgctg gggagtcac aatgaagaa gatttccga 
      aatgtgttc aataaggct gccttagag ttaaattac 
      acgccagtt ccggcccct aaaccggtc aaaaccggg 
      attttctcc aatcggaat cagttattt gaaaccatt 
      gaggagaag ccaatcgca aggcccgac tcacttccg 
      gtaggggag gagtattac atacagttt ccgcgatcg 
      ggcttacca gtggcagga gttgaggag aaagccggc 
      caagagtac atgaacgtg ggggctgcc aaagacgaa 
      gagacccac gaatacatg aacttcaaa gtaatgacc 
      aagagatag actaagact tgatgtaat tttttatgt 
      cagtaattt ttagtaaca taatattta tgtgaacct 
      ttttctcaa atatttatt tattcattt ttctgttat 
      attttatgc tccacgatt tgtatgttg ttaaattta 
      tattttgta catttttat aataaacgc aacgcattt 
      tt