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Gene information
General information
TC011317 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 3208824..3214475
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011317
    iBeetle screen iB_02630

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011317 RA
      MALGLSHEE TLKRYLYTC SYYGAFITG NPDKHFGNS 
      TGRLTFRWA KTAPEEFVK IITTVSEDA LLVRRSNFF 
      YLLAEGLLD KEVPSELKA EIKKLVFKL IKTDQDFFD 
      FIKFYTTLR TNKPKISTT LRKMIIKFY QNKEPKEFA 
      ETVARQESY HGWTHKDLI KLTHFKCNN VACEPIMKY 
      VLYGIDQLP EGSDETSKS IIEYLKKSH ELRTTEDVA 
      KATSLIKEL HVTVDRVNS KLNKTEEVW LAAIPEMTT 
      REVLQCLYR FYKFGFFKA GSQFQAKIC EALSDAEKI 
      KKCNLHPVE VFIYLKFFE KGGKTMDPK LLEYLQKKE 
      IREETLKRL TTPSEPKCK PVFQSINKC LKLSYDNVQ 
      PTGKRYMVT IDVTEKTET VCFHNKRIP CLEAAVAII 
      RFLSKVERN VTVAVFKDS QINFVDISK SHSAVDKLF 
      EHKSAYISP SAPLDWARN KKKQIDVFI NFMSSNWQA 
      NVPPEIKEK MEKVTEALS KYSKKMQSP ETRLVKIYL 
      DGAGGAFGE NTKNILSIA GFSVDVPKV LEAFCRGRF 
      C
    CDS sequence
    • >TC011317 RA
      atggctcta ggactctcg catgaagag accctcaaa 
      cgctatctc tacacctgc agttactac ggtgctttt 
      ataacggga aaccccgac aaacacttc ggcaattcc 
      actggacgt cttactttt cgctgggcg aagacagcc 
      cccgaagag ttcgtcaag atcataaca accgtcagt 
      gaagatgct ctattagtg agacgctcg aattttttc 
      tatttactg gctgaaggt ttactcgat aaagaggta 
      ccaagcgaa ttaaaggcc gaaataaag aaactcgtt 
      tttaaattg atcaaaacc gaccaagat tttttcgat 
      tttattaaa ttttacacg acactacga accaataaa 
      ccaaaaata tcaacgact ttgcgaaaa atgattatt 
      aagttttac caaaacaag gaaccgaaa gagtttgcc 
      gaaactgtg gccagacag gagagttat catgggtgg 
      acgcataag gatctcatc aaattgact cactttaaa 
      tgcaataat gtggcgtgt gaaccgatc atgaagtac 
      gtcctttac ggcatcgac cagcttccc gaaggctca 
      gatgagacc tcaaaatcc ataatcgag tatttgaaa 
      aaatcccac gaattgcga acgaccgaa gacgtagcc 
      aaagctacc tctcttatc aaagaactt cacgtcact 
      gtcgaccgt gttaattcg aaattaaac aaaaccgaa 
      gaagtttgg ttggcagca attcccgaa atgaccacc 
      cgtgaagtt ctacagtgc ctctaccga ttttacaaa 
      tttggtttt tttaaagct ggaagccag tttcaggca 
      aaaatttgc gaagctttg tctgacgct gagaaaatc 
      aaaaagtgc aatttgcat ccggttgaa gtgttcatt 
      tatttgaag tttttcgaa aaaggggga aaaacaatg 
      gaccccaag ctactcgaa tacttacaa aagaaggaa 
      atcagggag gaaaccttg aaacgatta acaactccg 
      tcagaaccc aagtgcaaa cccgtcttt cagagtatt 
      aacaaatgt ctcaaactg tcatacgac aacgtccag 
      ccaactggg aaaagatac atggtgaca atcgacgtg 
      accgaaaaa accgaaaca gtgtgtttt cataacaag 
      cgaataccg tgtctggaa gcagccgtc gcgattata 
      cgatttttg tcgaaagtc gagcgaaac gtcaccgtt 
      gctgtcttc aaagacagt caaatcaac ttcgtcgac 
      atcagcaaa tctcactct gccgttgac aagttattc 
      gaacacaag tcggcctat atttcgccc agcgccccc 
      cttgattgg gcccgcaat aaaaagaaa caaatcgac 
      gttttcatc aatttcatg agctcgaac tggcaggca 
      aacgtcccg ccagaaatc aaggaaaaa atggagaaa 
      gtgaccgaa gctttgagc aaatactcg aagaaaatg 
      caatcaccg gaaacccgt ttggtgaag atctatcta 
      gatggcgct gggggcgcg tttggtgag aacaccaag 
      aatatactg tccatagcc ggcttcagc gttgatgtt 
      cctaaagta ttggaagcg ttctgtaga gggcgcttc 
      tgttga
    mRNA sequence
    • >TC011317 RA
      tttttgtac actcagatg ttttcagtt cagatagaa 
      aattgtgca tcttcaata acaaggtaa aataaataa 
      atagaagag tattttaat caaaaacga cttaaataa 
      atcaaaatc aataaatat tgtgcattt ctggacgcc 
      agtattaca taatcaagt tgtttaaat aaaatggct 
      ctaggactc tcgcatgaa gagaccctc aaacgctat 
      ctctacacc tgcagttac tacggtgct tttataacg 
      ggaaacccc gacaaacac ttcggcaat tccactgga 
      cgtcttact tttcgctgg gcgaagaca gcccccgaa 
      gagttcgtc aagatcata acaaccgtc agtgaagat 
      gctctatta gtgagacgc tcgaatttt ttctattta 
      ctggctgaa ggtttactc gataaagag gtaccaagc 
      gaattaaag gccgaaata aagaaactc gtttttaaa 
      ttgatcaaa accgaccaa gattttttc gattttatt 
      aaattttac acgacacta cgaaccaat aaaccaaaa 
      atatcaacg actttgcga aaaatgatt attaagttt 
      taccaaaac aaggaaccg aaagagttt gccgaaact 
      gtggccaga caggagagt tatcatggg tggacgcat 
      aaggatctc atcaaattg actcacttt aaatgcaat 
      aatgtggcg tgtgaaccg atcatgaag tacgtcctt 
      tacggcatc gaccagctt cccgaaggc tcagatgag 
      acctcaaaa tccataatc gagtatttg aaaaaatcc 
      cacgaattg cgaacgacc gaagacgta gccaaagct 
      acctctctt atcaaagaa cttcacgtc actgtcgac 
      cgtgttaat tcgaaatta aacaaaacc gaagaagtt 
      tggttggca gcaattccc gaaatgacc acccgtgaa 
      gttctacag tgcctctac cgattttac aaatttggt 
      ttttttaaa gctggaagc cagtttcag gcaaaaatt 
      tgcgaagct ttgtctgac gctgagaaa atcaaaaag 
      tgcaatttg catccggtt gaagtgttc atttatttg 
      aagtttttc gaaaaaggg ggaaaaaca atggacccc 
      aagctactc gaatactta caaaagaag gaaatcagg 
      gaggaaacc ttgaaacga ttaacaact ccgtcagaa 
      cccaagtgc aaacccgtc tttcagagt attaacaaa 
      tgtctcaaa ctgtcatac gacaacgtc cagccaact 
      gggaaaaga tacatggtg acaatcgac gtgaccgaa 
      aaaaccgaa acagtgtgt tttcataac aagcgaata 
      ccgtgtctg gaagcagcc gtcgcgatt atacgattt 
      ttgtcgaaa gtcgagcga aacgtcacc gttgctgtc 
      ttcaaagac agtcaaatc aacttcgtc gacatcagc 
      aaatctcac tctgccgtt gacaagtta ttcgaacac 
      aagtcggcc tatatttcg cccagcgcc ccccttgat 
      tgggcccgc aataaaaag aaacaaatc gacgttttc 
      atcaatttc atgagctcg aactggcag gcaaacgtc 
      ccgccagaa atcaaggaa aaaatggag aaagtgacc 
      gaagctttg agcaaatac tcgaagaaa atgcaatca 
      ccggaaacc cgtttggtg aagatctat ctagatggc 
      gctgggggc gcgtttggt gagaacacc aagaatata 
      ctgtccata gccggcttc agcgttgat gttcctaaa 
      gtattggaa gcgttctgt agagggcgc ttctgttga 
      aaattctcg actgttttc cctcctaca agtgttcag 
      tcaaatcga cgatatttt ttttataat tgattttac 
      gcgagcgaa actttcgaa atcctgaat gttttaggt 
      gtaatttta gttatattt aacaaataa cttttctag 
      cgaatattt acgaactga tctggaccg tttgatgtt 
      gcgtagtta ggtttttat gttttcttt aattaagtt 
      accaaattt cgcttcatg tttgtttag cagagattc 
      catgactct ttcaataat aaatctggg tgcctttgg 
      agagtttgg aagtctgtt tagtgatta gttgtattg 
      acaggttca gtgtcctga ttattttaa cgcacaatc 
      ggattattg gacttggtt ttgttaggt tagtttttt 
      taaactaaa tctcatggt tatcgactt acctactga 
      taaattgtc tgtgaatgc atttttgaa atatcgtta 
      aatttgcta tttattacg gttagaatt ggtttttat 
      ttatttttg atacactta tgtatgaca cacaatgat 
      ttacaattc ctgtatgtt ttggaaaaa taataaaaa 
      tttacaagg act
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