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Gene information
General information
TC034900 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid72: 509475..555959
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034900
    iBeetle screen iB_02628
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 11.11% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Pupal morphology
    wings slightly dorsalnot closed - Number of animals showing the phenotype: 9

    Adult morphology
    elytra partially blistered - Number of animals showing the phenotype: 3


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034900 RA
      MTTATESMN WGTELWDQY DNLSIHTMK GIDFLEKYG 
      HFVKDRAAI EYEYAAKLR RLVKTYQPK KKDEDDYQF 
      TSCKAFKML MNEINDLAG QHEVVAENL QANVIKELN 
      ALVKDIREE RKKQLQEGN RLTQALQTQ LENLQRSKK 
      AYDKAFRDN EKALENFQK ADADLHLSR AEVEKQRAN 
      LAYKTQVCD TTKNEYAMQ LQRTNELQR QHFRSGLPE 
      VFRQLQELD EKRIKNIKN FIRSSVDIE RNVFPIINK 
      CLDGILKAA DVINEKEDT ILVIEKYKS GFLPPEDIP 
      FEDLSKGGS DSGSANNIN NIQISGKLK DGGYTVKGT 
      ITGKAMKKR AGIFHIFGS KGKLTEVCM AIKNAAISD 
      GKEDLSDLP PNQRRKKLM QKVEELKSK VAQETAAKD 
      GLMKMKGVY EANPALGDP MTIEGQLNE CSHRLDKLQ 
      GELKRYQSY LDDAIKGIQ SGQNNSLTN SPRLVNGSR 
      HHRNSGGSA AEEESLSRS ASDSSVTNP TINHNKQSA 
      PGTPQLNHG WVLVNNLYI R
    • >TC034900 RB
      MTTATESMN WGTELWDQY DNLSIHTMK GIDFLEKYG 
      HFVKDRAAI EYEYAAKLR RLVKTYQPK KKDEDDYQF 
      TSCKAFKML MNEINDLAG QHEVVAENL QANVIKELN 
      ALVKDIREE RKKQLQEGN RLTQALQTQ LENLQRSKK 
      AYDKAFRDN EKALENFQK ADADLHLSR AEVEKQRAN 
      LAYKTQVCD TTKNEYAMQ LQRTNELQR QHFRSGLPE 
      VFRQLQELD EKRIKNIKN FIRSSVDIE RNVFPIINK 
      CLDGILKAA DVINEKEDT ILVIEKYKS GFLPPEDIP 
      FEDLSKGGS DSGSANNIN NIQISGKLK DGGYTVKGT 
      ITGKAMKKR AGIFHIFGS KGNAAISDG KEDLSDLPP 
      NQRRKKLMQ KVEELKSKV AQETAAKDG LMKMKGVYE 
      ANPALGDPM TIEGQLNEC SHRLDKLQG ELKRYQSYL 
      DDAIKGIQS GQNNSLTNS PRLVNGSRH HRNSGGSAA 
      EEESLSRSA SDSSVTNPT INHNKQSAP GTPQLNHGW 
      VLVNNLYIR
    CDS sequence
    • >TC034900 RA
      atgacgacc gcaaccgaa agcatgaac tggggcacc 
      gagctctgg gaccagtat gacaatttg tcgattcat 
      acaatgaaa gggatcgat tttttggaa aaatacggt 
      catttcgtc aaagacagg gcggccata gagtacgaa 
      tatgcggcg aaattgagg agattagtg aagacgtac 
      caacccaag aaaaaggat gaagacgac taccagttt 
      acgtcgtgt aaagcgttc aagatgtta atgaacgaa 
      atcaatgac ctcgctgga caacacgag gtcgttgcc 
      gaaaatctt caagccaat gttatcaag gaattgaat 
      gcgttagtc aaagacatc agggaagaa cgaaaaaaa 
      cagctgcag gagggcaac cgcctgacc caagcctta 
      cagacccaa ttagaaaat ttacaaaga tcgaaaaaa 
      gcctacgac aaggcgttt cgcgataac gagaaagct 
      ttagaaaat tttcaaaaa gcagacgcc gatttgcat 
      ttatctcga gctgaggtt gaaaaacaa cgtgcgaat 
      ttagcttac aaaactcaa gtttgtgat acgacaaaa 
      aatgaatac gcgatgcag ttgcaaaga acaaacgaa 
      ttacaacga cagcacttt agatctgga ttgccggag 
      gtttttcgg cagttgcaa gagctggac gagaaacgg 
      attaaaaat attaaaaat tttatacgt agttctgta 
      gatatcgaa cgtaatgtg tttcctatt attaataaa 
      tgtttagat ggtattttg aaagccgcg gacgttatt 
      aatgaaaaa gaggacacg atacttgta atagaaaaa 
      tacaagtca ggctttcta cccccggag gatattccg 
      tttgaagat ttatcgaaa ggtggtagt gattcgggt 
      agtgctaat aacattaac aatattcaa attagtggt 
      aaattaaaa gatggtggt tacacagtg aaagggaca 
      ataacgggg aaagcgatg aagaaacgt gccgggatt 
      tttcacatt tttggaagc aaagggaag cttacagaa 
      gtctgtatg gccatcaag aacgctgct atttcggac 
      ggaaaggag gacctaagt gacctgcct ccaaaccag 
      cggcggaaa aaactcatg caaaaggtc gaagagctc 
      aaaagtaag gtcgcgcaa gaaactgcg gccaaagat 
      gggcttatg aaaatgaag ggagtgtat gaggccaac 
      cctgcattg ggcgatcca atgaccatc gaaggtcag 
      ctcaatgag tgcagccac agactggat aagctccaa 
      ggcgaattg aagaggtat cagagttat ctggatgat 
      gctatcaag ggaatccaa tcgggtcag aataactct 
      ttgacgaat tcgcctcga ttggtgaat gggtcgaga 
      catcaccga aattcggga ggaagtgct gccgaagag 
      gaatcactc agcaggtct gcaagtgac agtagcgtc 
      actaaccct actatcaat cataataag cagtccgca 
      ccgggcacg cctcagctt aatcatggg tgggtgttg 
      gttaataat ctgtatata agataa
    • >TC034900 RB
      atgacgacc gcaaccgaa agcatgaac tggggcacc 
      gagctctgg gaccagtat gacaatttg tcgattcat 
      acaatgaaa gggatcgat tttttggaa aaatacggt 
      catttcgtc aaagacagg gcggccata gagtacgaa 
      tatgcggcg aaattgagg agattagtg aagacgtac 
      caacccaag aaaaaggat gaagacgac taccagttt 
      acgtcgtgt aaagcgttc aagatgtta atgaacgaa 
      atcaatgac ctcgctgga caacacgag gtcgttgcc 
      gaaaatctt caagccaat gttatcaag gaattgaat 
      gcgttagtc aaagacatc agggaagaa cgaaaaaaa 
      cagctgcag gagggcaac cgcctgacc caagcctta 
      cagacccaa ttagaaaat ttacaaaga tcgaaaaaa 
      gcctacgac aaggcgttt cgcgataac gagaaagct 
      ttagaaaat tttcaaaaa gcagacgcc gatttgcat 
      ttatctcga gctgaggtt gaaaaacaa cgtgcgaat 
      ttagcttac aaaactcaa gtttgtgat acgacaaaa 
      aatgaatac gcgatgcag ttgcaaaga acaaacgaa 
      ttacaacga cagcacttt agatctgga ttgccggag 
      gtttttcgg cagttgcaa gagctggac gagaaacgg 
      attaaaaat attaaaaat tttatacgt agttctgta 
      gatatcgaa cgtaatgtg tttcctatt attaataaa 
      tgtttagat ggtattttg aaagccgcg gacgttatt 
      aatgaaaaa gaggacacg atacttgta atagaaaaa 
      tacaagtca ggctttcta cccccggag gatattccg 
      tttgaagat ttatcgaaa ggtggtagt gattcgggt 
      agtgctaat aacattaac aatattcaa attagtggt 
      aaattaaaa gatggtggt tacacagtg aaagggaca 
      ataacgggg aaagcgatg aagaaacgt gccgggatt 
      tttcacatt tttggaagc aaagggaac gctgctatt 
      tcggacgga aaggaggac ctaagtgac ctgcctcca 
      aaccagcgg cggaaaaaa ctcatgcaa aaggtcgaa 
      gagctcaaa agtaaggtc gcgcaagaa actgcggcc 
      aaagatggg cttatgaaa atgaaggga gtgtatgag 
      gccaaccct gcattgggc gatccaatg accatcgaa 
      ggtcagctc aatgagtgc agccacaga ctggataag 
      ctccaaggc gaattgaag aggtatcag agttatctg 
      gatgatgct atcaaggga atccaatcg ggtcagaat 
      aactctttg acgaattcg cctcgattg gtgaatggg 
      tcgagacat caccgaaat tcgggagga agtgctgcc 
      gaagaggaa tcactcagc aggtctgca agtgacagt 
      agcgtcact aaccctact atcaatcat aataagcag 
      tccgcaccg ggcacgcct cagcttaat catgggtgg 
      gtgttggtt aataatctg tatataaga taa
    mRNA sequence
    • >TC034900 RA
      tctaaagtg aaacagcca tgttgggta gtgacggtt 
      gaagaaaat cccagtgtt ggttataag caaattgtt 
      tgatgtgtt gttgagtgt taagttatt gttattgga 
      ttaatgacg accgcaacc gaaagcatg aactggggc 
      accgagctc tgggaccag tatgacaat ttgtcgatt 
      catacaatg aaagggatc gattttttg gaaaaatac 
      ggtcatttc gtcaaagac agggcggcc atagagtac 
      gaatatgcg gcgaaattg aggagatta gtgaagacg 
      taccaaccc aagaaaaag gatgaagac gactaccag 
      tttacgtcg tgtaaagcg ttcaagatg ttaatgaac 
      gaaatcaat gacctcgct ggacaacac gaggtcgtt 
      gccgaaaat cttcaagcc aatgttatc aaggaattg 
      aatgcgtta gtcaaagac atcagggaa gaacgaaaa 
      aaacagctg caggagggc aaccgcctg acccaagcc 
      ttacagacc caattagaa aatttacaa agatcgaaa 
      aaagcctac gacaaggcg tttcgcgat aacgagaaa 
      gctttagaa aattttcaa aaagcagac gccgatttg 
      catttatct cgagctgag gttgaaaaa caacgtgcg 
      aatttagct tacaaaact caagtttgt gatacgaca 
      aaaaatgaa tacgcgatg cagttgcaa agaacaaac 
      gaattacaa cgacagcac tttagatct ggattgccg 
      gaggttttt cggcagttg caagagctg gacgagaaa 
      cggattaaa aatattaaa aattttata cgtagttct 
      gtagatatc gaacgtaat gtgtttcct attattaat 
      aaatgttta gatggtatt ttgaaagcc gcggacgtt 
      attaatgaa aaagaggac acgatactt gtaatagaa 
      aaatacaag tcaggcttt ctacccccg gaggatatt 
      ccgtttgaa gatttatcg aaaggtggt agtgattcg 
      ggtagtgct aataacatt aacaatatt caaattagt 
      ggtaaatta aaagatggt ggttacaca gtgaaaggg 
      acaataacg gggaaagcg atgaagaaa cgtgccggg 
      atttttcac atttttgga agcaaaggg aagcttaca 
      gaagtctgt atggccatc aagaacgct gctatttcg 
      gacggaaag gaggaccta agtgacctg cctccaaac 
      cagcggcgg aaaaaactc atgcaaaag gtcgaagag 
      ctcaaaagt aaggtcgcg caagaaact gcggccaaa 
      gatgggctt atgaaaatg aagggagtg tatgaggcc 
      aaccctgca ttgggcgat ccaatgacc atcgaaggt 
      cagctcaat gagtgcagc cacagactg gataagctc 
      caaggcgaa ttgaagagg tatcagagt tatctggat 
      gatgctatc aagggaatc caatcgggt cagaataac 
      tctttgacg aattcgcct cgattggtg aatgggtcg 
      agacatcac cgaaattcg ggaggaagt gctgccgaa 
      gaggaatca ctcagcagg tctgcaagt gacagtagc 
      gtcactaac cctactatc aatcataat aagcagtcc 
      gcaccgggc acgcctcag cttaatcat gggtgggtg 
      ttggttaat aatctgtat ataagataa tatgcttca 
      gcttttctg catttattt tacgaaatt aaacccaga 
      aattttta
    • >TC034900 RB
      tctaaagtg aaacagcca tgttgggta gtgacggtt 
      gaagaaaat cccagtgtt ggttataag caaattgtt 
      tgatgtgtt gttgagtgt taagttatt gttattgga 
      ttaatgacg accgcaacc gaaagcatg aactggggc 
      accgagctc tgggaccag tatgacaat ttgtcgatt 
      catacaatg aaagggatc gattttttg gaaaaatac 
      ggtcatttc gtcaaagac agggcggcc atagagtac 
      gaatatgcg gcgaaattg aggagatta gtgaagacg 
      taccaaccc aagaaaaag gatgaagac gactaccag 
      tttacgtcg tgtaaagcg ttcaagatg ttaatgaac 
      gaaatcaat gacctcgct ggacaacac gaggtcgtt 
      gccgaaaat cttcaagcc aatgttatc aaggaattg 
      aatgcgtta gtcaaagac atcagggaa gaacgaaaa 
      aaacagctg caggagggc aaccgcctg acccaagcc 
      ttacagacc caattagaa aatttacaa agatcgaaa 
      aaagcctac gacaaggcg tttcgcgat aacgagaaa 
      gctttagaa aattttcaa aaagcagac gccgatttg 
      catttatct cgagctgag gttgaaaaa caacgtgcg 
      aatttagct tacaaaact caagtttgt gatacgaca 
      aaaaatgaa tacgcgatg cagttgcaa agaacaaac 
      gaattacaa cgacagcac tttagatct ggattgccg 
      gaggttttt cggcagttg caagagctg gacgagaaa 
      cggattaaa aatattaaa aattttata cgtagttct 
      gtagatatc gaacgtaat gtgtttcct attattaat 
      aaatgttta gatggtatt ttgaaagcc gcggacgtt 
      attaatgaa aaagaggac acgatactt gtaatagaa 
      aaatacaag tcaggcttt ctacccccg gaggatatt 
      ccgtttgaa gatttatcg aaaggtggt agtgattcg 
      ggtagtgct aataacatt aacaatatt caaattagt 
      ggtaaatta aaagatggt ggttacaca gtgaaaggg 
      acaataacg gggaaagcg atgaagaaa cgtgccggg 
      atttttcac atttttgga agcaaaggg aacgctgct 
      atttcggac ggaaaggag gacctaagt gacctgcct 
      ccaaaccag cggcggaaa aaactcatg caaaaggtc 
      gaagagctc aaaagtaag gtcgcgcaa gaaactgcg 
      gccaaagat gggcttatg aaaatgaag ggagtgtat 
      gaggccaac cctgcattg ggcgatcca atgaccatc 
      gaaggtcag ctcaatgag tgcagccac agactggat 
      aagctccaa ggcgaattg aagaggtat cagagttat 
      ctggatgat gctatcaag ggaatccaa tcgggtcag 
      aataactct ttgacgaat tcgcctcga ttggtgaat 
      gggtcgaga catcaccga aattcggga ggaagtgct 
      gccgaagag gaatcactc agcaggtct gcaagtgac 
      agtagcgtc actaaccct actatcaat cataataag 
      cagtccgca ccgggcacg cctcagctt aatcatggg 
      tgggtgttg gttaataat ctgtatata agataatat 
      gcttcagct tttctgcat ttattttac gaaattaaa 
      cccagaaat tttta