| General information | |||
| TC031339
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
scaffold_seqid70: 80988..126267 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC031339 | ||
| General information | ||||
| iB_02626 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
- >TC031339 RA
MNLHHFATI FAAIVVPSL ICGIYEAWT SAYVPKLEI ENSFTNSDG SWITISLCV GGLTGALIS FPLFDKWGR KKSLLTTTI PFMVSPLLL AFGNSVAIF CAARFFAGM GIGGCLAII PQFVGEIAH ETVRGALGT CIYVLQVFG MLFINVIGS YLSIKTSSF ILFGIGVVY LLLFIFVVE SPYFLIMKG ENEGARKAL RIFRNGGDV DSEFKRISQ AVAEQIDNK GQFSDLFKI KTNRRALLI VFISVNAKQ LTGDFTLDT YSQTIFNQL CDIPPMMFA VIFYSTKLI VVFISSFFI DKIGRRPTF LISLFGSGV IFLIIAAYL HIALHTDVV ISDFVKKIM IFVMVLFTI FYSFLVAVP VLLLGELFP MNVKAFVSM LYVIHFFVA SFVATKLFQ VITDCEYLG MDVAFFAYS LSCFIHFLL VFKFVFETK GKTLEAIQE DLREQGRAP PMEEWQMQF LIVGVAEQC TGACRRRNT IAQAWGTQT PEFASPGAC PLLRRSSAI PHKGGSSIP LPGSALPSR RCPPDKVRP VAGSSRASS PALSMIPRG PPAQISSPY QTPPQKNSN SMLDKLKLF KSNDRPAPT TNGKRTSSS SGVSSARSE RSDSSASLE PSADVKPVK NAARLKQTR PTKTTPTKN SPNALKKDV LKKTATEVE KHAQKVANL ASSKLVEPK IKAKTEIKS GQGQNHLPT QNQHGTGIP KPTAAIKGT TKIVRDDKS FPTLKTPIS RENSQASIT HTKPAVALV SPMKNEKEP QLSESSHSA STGHHSNSS ESSVIYKPS SESSSEHHN IIPNRKDPI AYISEATED QTDKEKLLN CENQEPKNF FNEKLNFMR EGSQGDDET ALSIEPMRP LLRGYCSTL TLPPRQRHY QRVQQDPSS DYCEISLAN GYLSDGEML RNAPSRDNS DGYMSEGGS VLYARRLQT MPAHIPNGV HAPPHRFHI VDARQTWSL GLTEILLSK VTLAGTKVG GFLCSKVIK KGNCDAISI GGDKDEDKA GKRCTPRIS PGLTVLTEQ SSRRGRVEE PPPPPAPRA ASRSSRNGV ALQDTKHGQ WKRYTDSPG VGSPPPPAP APSSPTSSR RERRSSPHG SKEKSSKVR GVPQSFGYV KRSSSTSNG TNGTPNGTT PQVLQNQTG KTASVSAVP RTKVKVSGG TQTCSSDLQ QSHKSNAPQ FKSYSLTGN AASQLSQSV RERLMMGSQ SLPKGAGQD YGLVLRQGR PKASDGSLS DTQAIENTS PYAPWLRHS NTYSVAPRL SETDSMESL TSLPGHRSS LTHTRLLRD SPSRLSRSN SIRSTKSEK LYPSMLHRN TEPETEPYY CLPIGALSH WSQPTSPAP GNARTFPLS PTHSSTPRH SIPKNDDVH GSSISLVST ASSLYSSAE EKQAHEIRK LRRELTEAQ EKVQTLTSQ LSTNVSLKP FATQAFN
- >TC031339 RA
atgaacctc caccatttt gcaacgatt tttgccgca atcgttgtc ccttcattg atttgcggc atttacgaa gcctggacc tctgcctac gtcccaaaa ctggaaatc gaaaattcc ttcacaaat tcggacggt tcctggatc accatatca ctgtgtgtt ggcggcctc acaggagcc ctaatctct ttcccactt tttgacaaa tgggggcgg aaaaaatca cttctgacc accacaatt cccttcatg gtgtcacct ctactcttg gcttttggc aattcggtt gcgattttt tgcgcagcg agatttttc gcaggaatg ggaattggc ggttgtttg gcaattata ccgcagttt gtgggcgaa atagcgcat gagacggtc agaggggct ttgggcacg tgtatctac gttttgcaa gttttcgga atgttgttt atcaacgtg attggctcg tatttgagc atcaagacc agttctttc atcttgttt ggtattgga gttgtgtat ttgttgctt tttattttt gtggttgaa agtccgtat tttctgatc atgaagggg gagaatgag ggggccaga aaggctcta cgtattttt cgaaatgga ggcgatgtt gatagcgag tttaagagg ataagtcaa gcagtggct gagcaaatt gacaataaa ggccaattt tccgattta ttcaaaatc aaaacgaac cgaagagct ttgctgatt gtattcatt tcggtcaac gccaaacaa ctaactggt gatttcacc ttagacact tactcccaa acaattttc aaccaactt tgcgacatt cctccaatg atgtttgct gtgattttt tactcaacc aaattgatc gtagtcttc atttcgtct tttttcatt gataaaatc ggacgtcgt ccaacattt ctcatttcc ctctttggt tcaggtgtg attttttta attatcgcc gcttactta cacattgct ctgcacaca gatgtggtc attagtgac tttgtcaag aaaataatg atttttgtt atggtgttg tttaccata ttttatagt tttcttgta gccgttccg gtattgttg ttgggtgag ttgtttcca atgaacgtg aaagctttt gtttcgatg ttgtatgtt atacatttt ttcgtagcg tcgtttgtg gccaccaaa ctgttccag gttataacc gactgtgaa tatctcggt atggatgtt gcgtttttt gcctactcg ttgtcctgt tttattcat tttttattg gtgtttaag tttgtgttt gagacgaag gggaaaaca ttggaggca atacaagaa gacttacgg gaacagggg agggctcct ccgatggaa gagtggcag atgcagttt ctcattgtt ggggtggct gaacagtgc acgggcgcg tgtcgtcgt cgtaacacc atcgctcaa gcatgggga acacaaact ccggagttc gcaagtcca ggagcttgc ccacttctc cggagatcg tcggccata cctcacaag ggcggcagc agcatcccc ttacccggc tcagccctg ccttcccga cgatgtccc ccagataaa gtgcgtccg gtggccggc tccagccgg gcctcaagt ccggccctg tccatgatc ccgcggggc cctccggcc caaatttcc agcccttac caaaccccc cctcagaaa aactccaac tcgatgctc gacaaactg aaacttttc aaatccaac gaccggcct gcgcccaca actaatggg aaaaggacc agcagttcc agtggggtc tcatctgcg aggagtgaa cgtagtgat agtagtgcc agtttggaa cctagtgct gacgtgaaa ccggtgaaa aacgccgct aggttgaag cagaccagg cctacgaag acgacgccc acgaagaat agtccaaat gccctgaag aaggacgtg ctgaagaag acggccact gaggtggag aaacatgcg cagaaagtg gcaaatttg gccagttcg aagctggtg gagcccaag atcaaggcg aagacggag attaagagt gggcaaggc cagaaccac ttgcccacg cagaaccaa cacggtacc ggtattccg aagccgacg gcagctatt aaaggtacc acaaaaatc gtccgcgac gacaagagc ttccccacc ctcaaaacc cccatctcc cgggaaaac tcccaagcc agcatcacg cacacgaaa ccggccgta gccctagtc tcccccatg aaaaacgaa aaagagccc caactctcc gaaagcagt cacagcgct tccacaggc caccacagc aactccagc gaaagcagc gtcatttac aagccttca agtgagagc agctccgaa caccacaac atcatccca aaccgcaag gacccaatc gcctacata tcagaagca acggaagac caaaccgac aaggaaaaa ctcctcaac tgcgaaaac caagaacct aagaacttt ttcaacgag aagttgaat ttcatgagg gaagggtcg caaggtgac gacgagact gcgctaagt atcgagccg atgcggccc cttctgagg ggctattgc agcactttg acgctccct ccgaggcag cggcactac caaagggtc cagcaggac ccgagttcg gactattgc gagatttcg ctcgctaat ggctacctt tctgatggt gaaatgttg cggaatgcg ccctcgagg gacaactcg gacgggtat atgtcggaa ggaggatca gttctgtac gcgcggagg ctccaaacc atgcctgct catattcca aatggggta catgcgccc cctcatcga tttcatatt gtagacgcc agacagaca tggtcactg ggcctaact gaaatatta ttatcgaaa gtcacactt gctgggacc aaagttggt ggttttttg tgctcgaaa gtaatcaaa aagggaaac tgtgatgcg atttctatc ggtggtgac aaggacgaa gacaaggcg ggaaagcgc tgcacgccg cggatttct cccggcctc acggtgttg acagagcag tcctcccgg cgggggcgc gtggaggag ccccctccc cctcctgca ccccgcgcc gcctcccgg tcctcccgc aacggcgtt gccttacaa gacaccaag cacggccaa tggaagcgc tacacggac tcgccaggc gtaggcagc cccccaccg ccagctcca gccccctcg agccccacc agcagccgc agggagcgc cgcagcagc ccccacggg agcaaggag aagagctcc aaggtgcgt ggagtaccg caaagtttt ggctatgtg aagaggagc agttcgaca agcaatgga accaatggg acaccaaat ggaacaaca cctcaagtt ttgcagaat caaacgggg aagacagct tcggtgtct gcagtgcct aggaccaag gttaaggtg tcgggaggg acgcagacg tgctccagt gatttgcaa cagtcgcac aaatcaaac gctccacaa ttcaaaagc tactctctg accggaaac gccgcaagt cagctcagc caatcggtg cgcgaacgc ttgatgatg ggttcacaa agtcttcca aagggcgct gggcaggac tatgggctt gttttgcga caaggacga cccaaagcc agtgatggc tctttatcc gacactcag gccattgaa aataccagt ccatacgcc ccctggctt cgacacagc aatacttac tcagttgcc ccccgattg tccgaaacc gacagcatg gaaagcctt acaagtctt cccggacac agaagcagt ctgacacac accagactc ctgagggat tccccgtct aggcttagt agaagtaac agcataagg tctaccaaa tccgaaaag ttgtaccct tctatgttg catcgtaac accgaacca gagacagaa ccgtactat tgtttacca attggggcc ctcagtcat tggtcccag cccacaagt ccagcacca ggaaatgca aggactttt cctttgtct ccaacgcat tcgtcgacg cctagacac agtataccc aaaaatgat gacgtccac ggttcctcc ataagtctg gtctcgacc gcctccagc ctctactcc tcggctgaa gagaaacaa gcccacgaa atccgaaaa ctccgccgc gaactgacc gaagctcag gagaaagtt caaacactg acatcgcag ctcagcacc aacgtaagt ctgaaacct ttcgctaca caagctttt aattaa
- >TC031339 RA
aataattcg ttgggtaaa cacaccacg atgaacctc caccatttt gcaacgatt tttgccgca atcgttgtc ccttcattg atttgcggc atttacgaa gcctggacc tctgcctac gtcccaaaa ctggaaatc gaaaattcc ttcacaaat tcggacggt tcctggatc accatatca ctgtgtgtt ggcggcctc acaggagcc ctaatctct ttcccactt tttgacaaa tgggggcgg aaaaaatca cttctgacc accacaatt cccttcatg gtgtcacct ctactcttg gcttttggc aattcggtt gcgattttt tgcgcagcg agatttttc gcaggaatg ggaattggc ggttgtttg gcaattata ccgcagttt gtgggcgaa atagcgcat gagacggtc agaggggct ttgggcacg tgtatctac gttttgcaa gttttcgga atgttgttt atcaacgtg attggctcg tatttgagc atcaagacc agttctttc atcttgttt ggtattgga gttgtgtat ttgttgctt tttattttt gtggttgaa agtccgtat tttctgatc atgaagggg gagaatgag ggggccaga aaggctcta cgtattttt cgaaatgga ggcgatgtt gatagcgag tttaagagg ataagtcaa gcagtggct gagcaaatt gacaataaa ggccaattt tccgattta ttcaaaatc aaaacgaac cgaagagct ttgctgatt gtattcatt tcggtcaac gccaaacaa ctaactggt gatttcacc ttagacact tactcccaa acaattttc aaccaactt tgcgacatt cctccaatg atgtttgct gtgattttt tactcaacc aaattgatc gtagtcttc atttcgtct tttttcatt gataaaatc ggacgtcgt ccaacattt ctcatttcc ctctttggt tcaggtgtg attttttta attatcgcc gcttactta cacattgct ctgcacaca gatgtggtc attagtgac tttgtcaag aaaataatg atttttgtt atggtgttg tttaccata ttttatagt tttcttgta gccgttccg gtattgttg ttgggtgag ttgtttcca atgaacgtg aaagctttt gtttcgatg ttgtatgtt atacatttt ttcgtagcg tcgtttgtg gccaccaaa ctgttccag gttataacc gactgtgaa tatctcggt atggatgtt gcgtttttt gcctactcg ttgtcctgt tttattcat tttttattg gtgtttaag tttgtgttt gagacgaag gggaaaaca ttggaggca atacaagaa gacttacgg gaacagggg agggctcct ccgatggaa gagtggcag atgcagttt ctcattgtt ggggtggct gaacagtgc acgggcgcg tgtcgtcgt cgtaacacc atcgctcaa gcatgggga acacaaact ccggagttc gcaagtcca ggagcttgc ccacttctc cggagatcg tcggccata cctcacaag ggcggcagc agcatcccc ttacccggc tcagccctg ccttcccga cgatgtccc ccagataaa gtgcgtccg gtggccggc tccagccgg gcctcaagt ccggccctg tccatgatc ccgcggggc cctccggcc caaatttcc agcccttac caaaccccc cctcagaaa aactccaac tcgatgctc gacaaactg aaacttttc aaatccaac gaccggcct gcgcccaca actaatggg aaaaggacc agcagttcc agtggggtc tcatctgcg aggagtgaa cgtagtgat agtagtgcc agtttggaa cctagtgct gacgtgaaa ccggtgaaa aacgccgct aggttgaag cagaccagg cctacgaag acgacgccc acgaagaat agtccaaat gccctgaag aaggacgtg ctgaagaag acggccact gaggtggag aaacatgcg cagaaagtg gcaaatttg gccagttcg aagctggtg gagcccaag atcaaggcg aagacggag attaagagt gggcaaggc cagaaccac ttgcccacg cagaaccaa cacggtacc ggtattccg aagccgacg gcagctatt aaaggtacc acaaaaatc gtccgcgac gacaagagc ttccccacc ctcaaaacc cccatctcc cgggaaaac tcccaagcc agcatcacg cacacgaaa ccggccgta gccctagtc tcccccatg aaaaacgaa aaagagccc caactctcc gaaagcagt cacagcgct tccacaggc caccacagc aactccagc gaaagcagc gtcatttac aagccttca agtgagagc agctccgaa caccacaac atcatccca aaccgcaag gacccaatc gcctacata tcagaagca acggaagac caaaccgac aaggaaaaa ctcctcaac tgcgaaaac caagaacct aagaacttt ttcaacgag aagttgaat ttcatgagg gaagggtcg caaggtgac gacgagact gcgctaagt atcgagccg atgcggccc cttctgagg ggctattgc agcactttg acgctccct ccgaggcag cggcactac caaagggtc cagcaggac ccgagttcg gactattgc gagatttcg ctcgctaat ggctacctt tctgatggt gaaatgttg cggaatgcg ccctcgagg gacaactcg gacgggtat atgtcggaa ggaggatca gttctgtac gcgcggagg ctccaaacc atgcctgct catattcca aatggggta catgcgccc cctcatcga tttcatatt gtagacgcc agacagaca tggtcactg ggcctaact gaaatatta ttatcgaaa gtcacactt gctgggacc aaagttggt ggttttttg tgctcgaaa gtaatcaaa aagggaaac tgtgatgcg atttctatc ggtggtgac aaggacgaa gacaaggcg ggaaagcgc tgcacgccg cggatttct cccggcctc acggtgttg acagagcag tcctcccgg cgggggcgc gtggaggag ccccctccc cctcctgca ccccgcgcc gcctcccgg tcctcccgc aacggcgtt gccttacaa gacaccaag cacggccaa tggaagcgc tacacggac tcgccaggc gtaggcagc cccccaccg ccagctcca gccccctcg agccccacc agcagccgc agggagcgc cgcagcagc ccccacggg agcaaggag aagagctcc aaggtgcgt ggagtaccg caaagtttt ggctatgtg aagaggagc agttcgaca agcaatgga accaatggg acaccaaat ggaacaaca cctcaagtt ttgcagaat caaacgggg aagacagct tcggtgtct gcagtgcct aggaccaag gttaaggtg tcgggaggg acgcagacg tgctccagt gatttgcaa cagtcgcac aaatcaaac gctccacaa ttcaaaagc tactctctg accggaaac gccgcaagt cagctcagc caatcggtg cgcgaacgc ttgatgatg ggttcacaa agtcttcca aagggcgct gggcaggac tatgggctt gttttgcga caaggacga cccaaagcc agtgatggc tctttatcc gacactcag gccattgaa aataccagt ccatacgcc ccctggctt cgacacagc aatacttac tcagttgcc ccccgattg tccgaaacc gacagcatg gaaagcctt acaagtctt cccggacac agaagcagt ctgacacac accagactc ctgagggat tccccgtct aggcttagt agaagtaac agcataagg tctaccaaa tccgaaaag ttgtaccct tctatgttg catcgtaac accgaacca gagacagaa ccgtactat tgtttacca attggggcc ctcagtcat tggtcccag cccacaagt ccagcacca ggaaatgca aggactttt cctttgtct ccaacgcat tcgtcgacg cctagacac agtataccc aaaaatgat gacgtccac ggttcctcc ataagtctg gtctcgacc gcctccagc ctctactcc tcggctgaa gagaaacaa gcccacgaa atccgaaaa ctccgccgc gaactgacc gaagctcag gagaaagtt caaacactg acatcgcag ctcagcacc aacgtaagt ctgaaacct ttcgctaca caagctttt aattaaaac accattcat aacaatttc aaaagcgat cgtagagta aaagtttct gtgatccga ttatgaaaa taggggctt tgtgtgggt ttttaatga tgaaacgaa cgtaatgcg acttaatac acgtatgtt ctgaaa