Status
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Gene information
General information
TC031339
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC016083,TC016082,TC016081
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 1394585..1439864
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031339
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02626

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031339 RA
      MNLHHFATI FAAIVVPSL ICGIYEAWT SAYVPKLEI 
      ENSFTNSDG SWITISLCV GGLTGALIS FPLFDKWGR 
      KKSLLTTTI PFMVSPLLL AFGNSVAIF CAARFFAGM 
      GIGGCLAII PQFVGEIAH ETVRGALGT CIYVLQVFG 
      MLFINVIGS YLSIKTSSF ILFGIGVVY LLLFIFVVE 
      SPYFLIMKG ENEGARKAL RIFRNGGDV DSEFKRISQ 
      AVAEQIDNK GQFSDLFKI KTNRRALLI VFISVNAKQ 
      LTGDFTLDT YSQTIFNQL CDIPPMMFA VIFYSTKLI 
      VVFISSFFI DKIGRRPTF LISLFGSGV IFLIIAAYL 
      HIALHTDVV ISDFVKKIM IFVMVLFTI FYSFLVAVP 
      VLLLGELFP MNVKAFVSM LYVIHFFVA SFVATKLFQ 
      VITDCEYLG MDVAFFAYS LSCFIHFLL VFKFVFETK 
      GKTLEAIQE DLREQGRAP PMEEWQMQF LIVGVAEQC 
      TGACRRRNT IAQAWGTQT PEFASPGAC PLLRRSSAI 
      PHKGGSSIP LPGSALPSR RCPPDKVRP VAGSSRASS 
      PALSMIPRG PPAQISSPY QTPPQKNSN SMLDKLKLF 
      KSNDRPAPT TNGKRTSSS SGVSSARSE RSDSSASLE 
      PSADVKPVK NAARLKQTR PTKTTPTKN SPNALKKDV 
      LKKTATEVE KHAQKVANL ASSKLVEPK IKAKTEIKS 
      GQGQNHLPT QNQHGTGIP KPTAAIKGT TKIVRDDKS 
      FPTLKTPIS RENSQASIT HTKPAVALV SPMKNEKEP 
      QLSESSHSA STGHHSNSS ESSVIYKPS SESSSEHHN 
      IIPNRKDPI AYISEATED QTDKEKLLN CENQEPKNF 
      FNEKLNFMR EGSQGDDET ALSIEPMRP LLRGYCSTL 
      TLPPRQRHY QRVQQDPSS DYCEISLAN GYLSDGEML 
      RNAPSRDNS DGYMSEGGS VLYARRLQT MPAHIPNGV 
      HAPPHRFHI VDARQTWSL GLTEILLSK VTLAGTKVG 
      GFLCSKVIK KGNCDAISI GGDKDEDKA GKRCTPRIS 
      PGLTVLTEQ SSRRGRVEE PPPPPAPRA ASRSSRNGV 
      ALQDTKHGQ WKRYTDSPG VGSPPPPAP APSSPTSSR 
      RERRSSPHG SKEKSSKVR GVPQSFGYV KRSSSTSNG 
      TNGTPNGTT PQVLQNQTG KTASVSAVP RTKVKVSGG 
      TQTCSSDLQ QSHKSNAPQ FKSYSLTGN AASQLSQSV 
      RERLMMGSQ SLPKGAGQD YGLVLRQGR PKASDGSLS 
      DTQAIENTS PYAPWLRHS NTYSVAPRL SETDSMESL 
      TSLPGHRSS LTHTRLLRD SPSRLSRSN SIRSTKSEK 
      LYPSMLHRN TEPETEPYY CLPIGALSH WSQPTSPAP 
      GNARTFPLS PTHSSTPRH SIPKNDDVH GSSISLVST 
      ASSLYSSAE EKQAHEIRK LRRELTEAQ EKVQTLTSQ 
      LSTNVSLKP FATQAFN
    CDS sequence
    • >TC031339 RA
      atgaacctc caccatttt gcaacgatt tttgccgca 
      atcgttgtc ccttcattg atttgcggc atttacgaa 
      gcctggacc tctgcctac gtcccaaaa ctggaaatc 
      gaaaattcc ttcacaaat tcggacggt tcctggatc 
      accatatca ctgtgtgtt ggcggcctc acaggagcc 
      ctaatctct ttcccactt tttgacaaa tgggggcgg 
      aaaaaatca cttctgacc accacaatt cccttcatg 
      gtgtcacct ctactcttg gcttttggc aattcggtt 
      gcgattttt tgcgcagcg agatttttc gcaggaatg 
      ggaattggc ggttgtttg gcaattata ccgcagttt 
      gtgggcgaa atagcgcat gagacggtc agaggggct 
      ttgggcacg tgtatctac gttttgcaa gttttcgga 
      atgttgttt atcaacgtg attggctcg tatttgagc 
      atcaagacc agttctttc atcttgttt ggtattgga 
      gttgtgtat ttgttgctt tttattttt gtggttgaa 
      agtccgtat tttctgatc atgaagggg gagaatgag 
      ggggccaga aaggctcta cgtattttt cgaaatgga 
      ggcgatgtt gatagcgag tttaagagg ataagtcaa 
      gcagtggct gagcaaatt gacaataaa ggccaattt 
      tccgattta ttcaaaatc aaaacgaac cgaagagct 
      ttgctgatt gtattcatt tcggtcaac gccaaacaa 
      ctaactggt gatttcacc ttagacact tactcccaa 
      acaattttc aaccaactt tgcgacatt cctccaatg 
      atgtttgct gtgattttt tactcaacc aaattgatc 
      gtagtcttc atttcgtct tttttcatt gataaaatc 
      ggacgtcgt ccaacattt ctcatttcc ctctttggt 
      tcaggtgtg attttttta attatcgcc gcttactta 
      cacattgct ctgcacaca gatgtggtc attagtgac 
      tttgtcaag aaaataatg atttttgtt atggtgttg 
      tttaccata ttttatagt tttcttgta gccgttccg 
      gtattgttg ttgggtgag ttgtttcca atgaacgtg 
      aaagctttt gtttcgatg ttgtatgtt atacatttt 
      ttcgtagcg tcgtttgtg gccaccaaa ctgttccag 
      gttataacc gactgtgaa tatctcggt atggatgtt 
      gcgtttttt gcctactcg ttgtcctgt tttattcat 
      tttttattg gtgtttaag tttgtgttt gagacgaag 
      gggaaaaca ttggaggca atacaagaa gacttacgg 
      gaacagggg agggctcct ccgatggaa gagtggcag 
      atgcagttt ctcattgtt ggggtggct gaacagtgc 
      acgggcgcg tgtcgtcgt cgtaacacc atcgctcaa 
      gcatgggga acacaaact ccggagttc gcaagtcca 
      ggagcttgc ccacttctc cggagatcg tcggccata 
      cctcacaag ggcggcagc agcatcccc ttacccggc 
      tcagccctg ccttcccga cgatgtccc ccagataaa 
      gtgcgtccg gtggccggc tccagccgg gcctcaagt 
      ccggccctg tccatgatc ccgcggggc cctccggcc 
      caaatttcc agcccttac caaaccccc cctcagaaa 
      aactccaac tcgatgctc gacaaactg aaacttttc 
      aaatccaac gaccggcct gcgcccaca actaatggg 
      aaaaggacc agcagttcc agtggggtc tcatctgcg 
      aggagtgaa cgtagtgat agtagtgcc agtttggaa 
      cctagtgct gacgtgaaa ccggtgaaa aacgccgct 
      aggttgaag cagaccagg cctacgaag acgacgccc 
      acgaagaat agtccaaat gccctgaag aaggacgtg 
      ctgaagaag acggccact gaggtggag aaacatgcg 
      cagaaagtg gcaaatttg gccagttcg aagctggtg 
      gagcccaag atcaaggcg aagacggag attaagagt 
      gggcaaggc cagaaccac ttgcccacg cagaaccaa 
      cacggtacc ggtattccg aagccgacg gcagctatt 
      aaaggtacc acaaaaatc gtccgcgac gacaagagc 
      ttccccacc ctcaaaacc cccatctcc cgggaaaac 
      tcccaagcc agcatcacg cacacgaaa ccggccgta 
      gccctagtc tcccccatg aaaaacgaa aaagagccc 
      caactctcc gaaagcagt cacagcgct tccacaggc 
      caccacagc aactccagc gaaagcagc gtcatttac 
      aagccttca agtgagagc agctccgaa caccacaac 
      atcatccca aaccgcaag gacccaatc gcctacata 
      tcagaagca acggaagac caaaccgac aaggaaaaa 
      ctcctcaac tgcgaaaac caagaacct aagaacttt 
      ttcaacgag aagttgaat ttcatgagg gaagggtcg 
      caaggtgac gacgagact gcgctaagt atcgagccg 
      atgcggccc cttctgagg ggctattgc agcactttg 
      acgctccct ccgaggcag cggcactac caaagggtc 
      cagcaggac ccgagttcg gactattgc gagatttcg 
      ctcgctaat ggctacctt tctgatggt gaaatgttg 
      cggaatgcg ccctcgagg gacaactcg gacgggtat 
      atgtcggaa ggaggatca gttctgtac gcgcggagg 
      ctccaaacc atgcctgct catattcca aatggggta 
      catgcgccc cctcatcga tttcatatt gtagacgcc 
      agacagaca tggtcactg ggcctaact gaaatatta 
      ttatcgaaa gtcacactt gctgggacc aaagttggt 
      ggttttttg tgctcgaaa gtaatcaaa aagggaaac 
      tgtgatgcg atttctatc ggtggtgac aaggacgaa 
      gacaaggcg ggaaagcgc tgcacgccg cggatttct 
      cccggcctc acggtgttg acagagcag tcctcccgg 
      cgggggcgc gtggaggag ccccctccc cctcctgca 
      ccccgcgcc gcctcccgg tcctcccgc aacggcgtt 
      gccttacaa gacaccaag cacggccaa tggaagcgc 
      tacacggac tcgccaggc gtaggcagc cccccaccg 
      ccagctcca gccccctcg agccccacc agcagccgc 
      agggagcgc cgcagcagc ccccacggg agcaaggag 
      aagagctcc aaggtgcgt ggagtaccg caaagtttt 
      ggctatgtg aagaggagc agttcgaca agcaatgga 
      accaatggg acaccaaat ggaacaaca cctcaagtt 
      ttgcagaat caaacgggg aagacagct tcggtgtct 
      gcagtgcct aggaccaag gttaaggtg tcgggaggg 
      acgcagacg tgctccagt gatttgcaa cagtcgcac 
      aaatcaaac gctccacaa ttcaaaagc tactctctg 
      accggaaac gccgcaagt cagctcagc caatcggtg 
      cgcgaacgc ttgatgatg ggttcacaa agtcttcca 
      aagggcgct gggcaggac tatgggctt gttttgcga 
      caaggacga cccaaagcc agtgatggc tctttatcc 
      gacactcag gccattgaa aataccagt ccatacgcc 
      ccctggctt cgacacagc aatacttac tcagttgcc 
      ccccgattg tccgaaacc gacagcatg gaaagcctt 
      acaagtctt cccggacac agaagcagt ctgacacac 
      accagactc ctgagggat tccccgtct aggcttagt 
      agaagtaac agcataagg tctaccaaa tccgaaaag 
      ttgtaccct tctatgttg catcgtaac accgaacca 
      gagacagaa ccgtactat tgtttacca attggggcc 
      ctcagtcat tggtcccag cccacaagt ccagcacca 
      ggaaatgca aggactttt cctttgtct ccaacgcat 
      tcgtcgacg cctagacac agtataccc aaaaatgat 
      gacgtccac ggttcctcc ataagtctg gtctcgacc 
      gcctccagc ctctactcc tcggctgaa gagaaacaa 
      gcccacgaa atccgaaaa ctccgccgc gaactgacc 
      gaagctcag gagaaagtt caaacactg acatcgcag 
      ctcagcacc aacgtaagt ctgaaacct ttcgctaca 
      caagctttt aattaa
    mRNA sequence
    • >TC031339 RA
      aataattcg ttgggtaaa cacaccacg atgaacctc 
      caccatttt gcaacgatt tttgccgca atcgttgtc 
      ccttcattg atttgcggc atttacgaa gcctggacc 
      tctgcctac gtcccaaaa ctggaaatc gaaaattcc 
      ttcacaaat tcggacggt tcctggatc accatatca 
      ctgtgtgtt ggcggcctc acaggagcc ctaatctct 
      ttcccactt tttgacaaa tgggggcgg aaaaaatca 
      cttctgacc accacaatt cccttcatg gtgtcacct 
      ctactcttg gcttttggc aattcggtt gcgattttt 
      tgcgcagcg agatttttc gcaggaatg ggaattggc 
      ggttgtttg gcaattata ccgcagttt gtgggcgaa 
      atagcgcat gagacggtc agaggggct ttgggcacg 
      tgtatctac gttttgcaa gttttcgga atgttgttt 
      atcaacgtg attggctcg tatttgagc atcaagacc 
      agttctttc atcttgttt ggtattgga gttgtgtat 
      ttgttgctt tttattttt gtggttgaa agtccgtat 
      tttctgatc atgaagggg gagaatgag ggggccaga 
      aaggctcta cgtattttt cgaaatgga ggcgatgtt 
      gatagcgag tttaagagg ataagtcaa gcagtggct 
      gagcaaatt gacaataaa ggccaattt tccgattta 
      ttcaaaatc aaaacgaac cgaagagct ttgctgatt 
      gtattcatt tcggtcaac gccaaacaa ctaactggt 
      gatttcacc ttagacact tactcccaa acaattttc 
      aaccaactt tgcgacatt cctccaatg atgtttgct 
      gtgattttt tactcaacc aaattgatc gtagtcttc 
      atttcgtct tttttcatt gataaaatc ggacgtcgt 
      ccaacattt ctcatttcc ctctttggt tcaggtgtg 
      attttttta attatcgcc gcttactta cacattgct 
      ctgcacaca gatgtggtc attagtgac tttgtcaag 
      aaaataatg atttttgtt atggtgttg tttaccata 
      ttttatagt tttcttgta gccgttccg gtattgttg 
      ttgggtgag ttgtttcca atgaacgtg aaagctttt 
      gtttcgatg ttgtatgtt atacatttt ttcgtagcg 
      tcgtttgtg gccaccaaa ctgttccag gttataacc 
      gactgtgaa tatctcggt atggatgtt gcgtttttt 
      gcctactcg ttgtcctgt tttattcat tttttattg 
      gtgtttaag tttgtgttt gagacgaag gggaaaaca 
      ttggaggca atacaagaa gacttacgg gaacagggg 
      agggctcct ccgatggaa gagtggcag atgcagttt 
      ctcattgtt ggggtggct gaacagtgc acgggcgcg 
      tgtcgtcgt cgtaacacc atcgctcaa gcatgggga 
      acacaaact ccggagttc gcaagtcca ggagcttgc 
      ccacttctc cggagatcg tcggccata cctcacaag 
      ggcggcagc agcatcccc ttacccggc tcagccctg 
      ccttcccga cgatgtccc ccagataaa gtgcgtccg 
      gtggccggc tccagccgg gcctcaagt ccggccctg 
      tccatgatc ccgcggggc cctccggcc caaatttcc 
      agcccttac caaaccccc cctcagaaa aactccaac 
      tcgatgctc gacaaactg aaacttttc aaatccaac 
      gaccggcct gcgcccaca actaatggg aaaaggacc 
      agcagttcc agtggggtc tcatctgcg aggagtgaa 
      cgtagtgat agtagtgcc agtttggaa cctagtgct 
      gacgtgaaa ccggtgaaa aacgccgct aggttgaag 
      cagaccagg cctacgaag acgacgccc acgaagaat 
      agtccaaat gccctgaag aaggacgtg ctgaagaag 
      acggccact gaggtggag aaacatgcg cagaaagtg 
      gcaaatttg gccagttcg aagctggtg gagcccaag 
      atcaaggcg aagacggag attaagagt gggcaaggc 
      cagaaccac ttgcccacg cagaaccaa cacggtacc 
      ggtattccg aagccgacg gcagctatt aaaggtacc 
      acaaaaatc gtccgcgac gacaagagc ttccccacc 
      ctcaaaacc cccatctcc cgggaaaac tcccaagcc 
      agcatcacg cacacgaaa ccggccgta gccctagtc 
      tcccccatg aaaaacgaa aaagagccc caactctcc 
      gaaagcagt cacagcgct tccacaggc caccacagc 
      aactccagc gaaagcagc gtcatttac aagccttca 
      agtgagagc agctccgaa caccacaac atcatccca 
      aaccgcaag gacccaatc gcctacata tcagaagca 
      acggaagac caaaccgac aaggaaaaa ctcctcaac 
      tgcgaaaac caagaacct aagaacttt ttcaacgag 
      aagttgaat ttcatgagg gaagggtcg caaggtgac 
      gacgagact gcgctaagt atcgagccg atgcggccc 
      cttctgagg ggctattgc agcactttg acgctccct 
      ccgaggcag cggcactac caaagggtc cagcaggac 
      ccgagttcg gactattgc gagatttcg ctcgctaat 
      ggctacctt tctgatggt gaaatgttg cggaatgcg 
      ccctcgagg gacaactcg gacgggtat atgtcggaa 
      ggaggatca gttctgtac gcgcggagg ctccaaacc 
      atgcctgct catattcca aatggggta catgcgccc 
      cctcatcga tttcatatt gtagacgcc agacagaca 
      tggtcactg ggcctaact gaaatatta ttatcgaaa 
      gtcacactt gctgggacc aaagttggt ggttttttg 
      tgctcgaaa gtaatcaaa aagggaaac tgtgatgcg 
      atttctatc ggtggtgac aaggacgaa gacaaggcg 
      ggaaagcgc tgcacgccg cggatttct cccggcctc 
      acggtgttg acagagcag tcctcccgg cgggggcgc 
      gtggaggag ccccctccc cctcctgca ccccgcgcc 
      gcctcccgg tcctcccgc aacggcgtt gccttacaa 
      gacaccaag cacggccaa tggaagcgc tacacggac 
      tcgccaggc gtaggcagc cccccaccg ccagctcca 
      gccccctcg agccccacc agcagccgc agggagcgc 
      cgcagcagc ccccacggg agcaaggag aagagctcc 
      aaggtgcgt ggagtaccg caaagtttt ggctatgtg 
      aagaggagc agttcgaca agcaatgga accaatggg 
      acaccaaat ggaacaaca cctcaagtt ttgcagaat 
      caaacgggg aagacagct tcggtgtct gcagtgcct 
      aggaccaag gttaaggtg tcgggaggg acgcagacg 
      tgctccagt gatttgcaa cagtcgcac aaatcaaac 
      gctccacaa ttcaaaagc tactctctg accggaaac 
      gccgcaagt cagctcagc caatcggtg cgcgaacgc 
      ttgatgatg ggttcacaa agtcttcca aagggcgct 
      gggcaggac tatgggctt gttttgcga caaggacga 
      cccaaagcc agtgatggc tctttatcc gacactcag 
      gccattgaa aataccagt ccatacgcc ccctggctt 
      cgacacagc aatacttac tcagttgcc ccccgattg 
      tccgaaacc gacagcatg gaaagcctt acaagtctt 
      cccggacac agaagcagt ctgacacac accagactc 
      ctgagggat tccccgtct aggcttagt agaagtaac 
      agcataagg tctaccaaa tccgaaaag ttgtaccct 
      tctatgttg catcgtaac accgaacca gagacagaa 
      ccgtactat tgtttacca attggggcc ctcagtcat 
      tggtcccag cccacaagt ccagcacca ggaaatgca 
      aggactttt cctttgtct ccaacgcat tcgtcgacg 
      cctagacac agtataccc aaaaatgat gacgtccac 
      ggttcctcc ataagtctg gtctcgacc gcctccagc 
      ctctactcc tcggctgaa gagaaacaa gcccacgaa 
      atccgaaaa ctccgccgc gaactgacc gaagctcag 
      gagaaagtt caaacactg acatcgcag ctcagcacc 
      aacgtaagt ctgaaacct ttcgctaca caagctttt 
      aattaaaac accattcat aacaatttc aaaagcgat 
      cgtagagta aaagtttct gtgatccga ttatgaaaa 
      taggggctt tgtgtgggt ttttaatga tgaaacgaa 
      cgtaatgcg acttaatac acgtatgtt ctgaaa
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