Status
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Gene information
General information
TC011255 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 716967..741214
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011255
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02625

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection



    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection



    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection



    Adult morphology
    abdominal odoriferous glands irregular - Number of animals showing the phenotype: 4 (41 dpi)
    Days post injection



    abdominal odoriferous glands partially content not present - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    abdominal odoriferous glands shape irregular - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011255 RA
      MLCFRIQQV DGGFFEKFG NLLREHAET TENRESEEK 
      RKVEQIAEC LRSDECAST DTEFVASSG DSDNEAKSL 
      SSDSEDEKD KTDLISAAF NFSLNGNVP RAKPRNNFR 
      GVKKDELRR QTSMYLASM TIDELYAEI LYEILHNVG 
      CDVSVEIGQ TALISYAQD AFKIPNNKH NQLLSEAEK 
      KEAPEFLIN VEVIEAKDL KPKDSNGLS DPFVTLYLA 
      SNTSHRYNT SVKYGTLVP TWEEHFALP ISDNSIDDT 
      LCIEVWDFD PAESVKEKF GRFFDVKGV KGMRKFVKE 
      IAVAAATGQ HENELIGVA KIPLNSIPA SGLTMWYSL 
      DKKNKATRQ GVIKIRIAF SAEKNRQVA AQEHRHMLR 
      IILLHELEL SKVAQFWWC GTFSPQGEA LLTQHIAQS 
      GLTPPEVAL CQWSVYTSV HQNHQLSFS LFSKLLEKL 
      AKPLRSEED LKIFWDGAK KLLPSCFSV IRKIRKRDF 
      KDKMTMQQL TEVLNILGN IKMMEMPAD VDLFPNNMY 
      PWVTFSEDT KRDILTVVA DAVQQGADD WFNHITENN 
      VQQVENDVG RLQYLIQAI QLVKSDLQK ALEFYDKLF 
      QQTINFQYA KALYVFYQA KISALAEPD VRNICKSLK 
      KLSFTGFTT NETDSNEPL KEGTTLFEL YLALHRFVV 
      LGKGLCQTD SDSFHIRNF YQWFHGGVA QWLDIAVFK 
      ALQRIEKAV ELDKLVPVD TTVRYSSSA VDTISIFYQ 
      VKVFWQQLN WPDVEGCYT FIAKIIDDI CRCCVFYAE 
      RMAMRVDGM GVQTDEKKF EVTNEWCLA INNIEYVRQ 
      SLQPFTDEL GMEGVIEKL ADLKSAMEA KRCQDTLNT 
      VIANAIDTV KNEIIALLE TVVKKMVPA MKRLLIEGA 
      ELFNQDSNS IDRLMLYVD SNLSTLHNE LNEENFNRV 
      LEIVWDNLD GILKEIIQS NLEKRRPPS FFANLHKTL 
      NLMLGSFKA NDLNCEELK KTELKLKVN GLETNDLIH 
      LVHLNLYEE FKNLKESKY GVLSVKAKF ENNNNLKVT 
      VMNARNLLA MDSNGYSDS FVRIHLLPE HKFSGIPKP 
      KTQTHYKTL FPLYDENFT VELPTDYKS VEDGLLLFS 
      VKDKDMLGY NNQYIGEAF LHFKDIDSS NTPMDDLPQ 
      ISLALGRPT DLSTEAIKA LESRLGDKQ AKEFLRKLK 
      QRMGTS
    CDS sequence
    • >TC011255 RA
      atgctgtgt ttcagaatt cagcaagtt gacggtggt 
      tttttcgag aaattcggc aacttattg cgtgagcat 
      gccgaaacg acggaaaac cgggaaagc gaagaaaaa 
      cgaaaagtt gaacaaatc gctgaatgt ctccgatct 
      gatgaatgc gccagcact gacactgaa ttcgttgct 
      tcttctggt gatagcgac aatgaagcc aaaagtttg 
      tcttcagac tcagaagac gaaaaggac aagactgat 
      ttaatttcc gctgcgttt aattttagc ttgaacgga 
      aacgttccg cgcgcaaaa cctcgaaat aatttcaga 
      ggtgttaaa aaagatgaa ttgcggcga caaacgagc 
      atgtatttg gcgtccatg acaatcgat gagctgtat 
      gctgaaatt ttgtacgaa atcttacat aatgttggc 
      tgtgatgta agtgttgag ataggacag actgcctta 
      atctcgtac gcacaagat gcatttaaa attcctaac 
      aataaacat aatcaacta ttgtctgaa gctgaaaag 
      aaggaggct cctgaattt ttgatcaat gtcgaagtt 
      atcgaagcc aaagatttg aaaccaaag gattccaac 
      gggttgagc gatccgttc gttacttta tacttagct 
      agtaataca tcacataga tataacact tcggtcaag 
      tatgggact ctggtgcca acgtgggaa gaacacttt 
      gctttaccg atctctgac aattcaatc gacgacacc 
      ctctgcatc gaggtgtgg gacttcgac cccgccgaa 
      tccgtcaaa gaaaaattc ggtcgtttc ttcgacgtg 
      aaaggtgtc aaaggcatg cgaaaattc gtcaaagag 
      atcgcagtg gccgctgcc accggccaa cacgaaaac 
      gaactaatc ggcgtcgcc aaaatcccc cttaactca 
      atcccggcc tcgggcctc accatgtgg tacagcctt 
      gacaagaag aacaaagcg actcgtcaa ggagtcatc 
      aaaatccga atcgccttc agtgcggaa aaaaaccga 
      caagtggcc gcacaagag cacagacac atgttacgc 
      atcattctt ttgcacgaa ctcgaacta tcaaaagtt 
      gcccagttc tggtggtgt ggcactttt agcccccaa 
      ggcgaagcg ttgctgacc cagcacata gcccagtct 
      gggctaaca ccaccggaa gtggctttg tgccaatgg 
      tcggtttac acttccgtc catcagaac caccagtta 
      agtttcagt ctgttttcg aaacttttg gagaaattg 
      gcgaaaccg cttcggagt gaagaagat ttgaagata 
      ttttgggac ggggcgaag aaattgcta ccgtcgtgt 
      ttctccgtc attcggaag atcaggaag agggatttt 
      aaggataaa atgacgatg caacaactg actgaagtt 
      ttgaatatt ttggggaat attaaaatg atggaaatg 
      ccagctgat gttgacttg ttcccgaac aatatgtat 
      ccatgggtg acttttagt gaagacacg aaacgtgat 
      attttgacg gttgttgct gatgctgtt cagcaaggg 
      gccgatgat tggtttaat catatcacc gagaataat 
      gttcaacaa gttgaaaat gatgtggga agattgcag 
      tacttgatc caagccatt cagttggtc aaaagcgat 
      ctgcaaaag gcgctggag ttttacgac aaactcttc 
      caacaaaca ataaacttc caatacgcc aaagcctta 
      tacgtcttc taccaagcg aaaatatca gcccttgcc 
      gaacctgac gtccgcaac atttgcaaa tccctaaag 
      aagctttct ttcaccggc ttcacaaca aacgaaacc 
      gactccaac gaaccgcta aaagaagga acaaccttg 
      ttcgaactg tatttagct ctccaccga tttgttgtt 
      cttggcaaa ggtttatgt cagacagat tcggattca 
      tttcatatt cggaatttt tatcaatgg tttcatggc 
      ggtgtggct caatggctc gatattgcc gtcttcaaa 
      gccctccaa cgtattgag aaagccgta gagttggat 
      aaattagtt cctgtggac accactgtt aggtacagc 
      tcttctgcc gtagacaca atctcgatt ttttaccaa 
      gtgaaagta ttttggcaa cagctcaac tggccggac 
      gttgaaggt tgttacacg ttcattgcc aaaataata 
      gatgatata tgccgttgt tgcgttttc tacgccgaa 
      aggatggca atgagggtc gacggaatg ggtgttcaa 
      acagacgag aagaaattt gaagtgaca aatgagtgg 
      tgtttggcc ataaataac atcgaatat gtgagacag 
      tcccttcaa ccttttacc gatgaactt ggcatggaa 
      ggagttatt gaaaaactg gccgatttg aaaagtgca 
      atggaagca aaacgatgc caagatacg ctgaatact 
      gtcatagca aatgctatt gataccgtc aaaaacgaa 
      atcatcgcg cttttggaa actgtggtt aaaaagatg 
      gtgccagca atgaaacga cttctgata gaaggcgct 
      gagttgttt aatcaggat agtaacagt attgataga 
      ctcatgtta tacgtagac agtaattta tcgacttta 
      cataacgag ctaaacgag gagaatttt aaccgagtc 
      ttggaaata gtgtgggat aatttagac ggaattttg 
      aaggaaata attcaatca aacttggag aaacgaaga 
      ccgccgtct ttcttcgcc aatcttcac aaaacatta 
      aacttaatg cttggctcg tttaaagcc aatgattta 
      aattgtgaa gaactgaag aaaaccgaa ctgaaattg 
      aaagtgaat gggcttgaa accaacgac ttgatccac 
      ttggttcat ctgaatttg tacgaagaa ttcaaaaat 
      ttgaaagag tcgaaatac ggggtcttg tccgtaaaa 
      gccaaattc gaaaacaac aacaacctc aaagtgact 
      gtaatgaac gcacgaaac ttattagcg atggattcg 
      aacggttat agcgactcg tttgtgagg attcatcta 
      ttaccagaa cataaattt agtgggatc cctaaaccc 
      aaaacgcaa actcattac aagactttg tttcctttg 
      tatgatgag aactttacc gtggagcta cccactgat 
      tacaaaagt gtagaagat ggactgtta ctttttagt 
      gtcaaagac aaagacatg ttgggttat aataaccaa 
      tacataggt gaagcgttt ctgcatttt aaagacata 
      gattcgtca aatactcca atggacgac ttaccccaa 
      atttcgtta gctttagga aggcctact gatttaagt 
      actgaagca ataaaggct ctagaaagt agactaggt 
      gataaacaa gccaaggaa tttttaaga aaacttaaa 
      caaagaatg ggaacatca taa
    mRNA sequence
    • >TC011255 RA
      atttgtgaa aaaactgct taatctaaa tagcggtaa 
      taatgctgt gtttcagaa ttcagcaag ttgacggtg 
      gttttttcg agaaattcg gcaacttat tgcgtgagc 
      atgccgaaa cgacggaaa accgggaaa gcgaagaaa 
      aacgaaaag ttgaacaaa tcgctgaat gtctccgat 
      ctgatgaat gcgccagca ctgacactg aattcgttg 
      cttcttctg gtgatagcg acaatgaag ccaaaagtt 
      tgtcttcag actcagaag acgaaaagg acaagactg 
      atttaattt ccgctgcgt ttaatttta gcttgaacg 
      gaaacgttc cgcgcgcaa aacctcgaa ataatttca 
      gaggtgtta aaaaagatg aattgcggc gacaaacga 
      gcatgtatt tggcgtcca tgacaatcg atgagctgt 
      atgctgaaa ttttgtacg aaatcttac ataatgttg 
      gctgtgatg taagtgttg agataggac agactgcct 
      taatctcgt acgcacaag atgcattta aaattccta 
      acaataaac ataatcaac tattgtctg aagctgaaa 
      agaaggagg ctcctgaat ttttgatca atgtcgaag 
      ttatcgaag ccaaagatt tgaaaccaa aggattcca 
      acgggttga gcgatccgt tcgttactt tatacttag 
      ctagtaata catcacata gatataaca cttcggtca 
      agtatggga ctctggtgc caacgtggg aagaacact 
      ttgctttac cgatctctg acaattcaa tcgacgaca 
      ccctctgca tcgaggtgt gggacttcg accccgccg 
      aatccgtca aagaaaaat tcggtcgtt tcttcgacg 
      tgaaaggtg tcaaaggca tgcgaaaat tcgtcaaag 
      agatcgcag tggccgctg ccaccggcc aacacgaaa 
      acgaactaa tcggcgtcg ccaaaatcc cccttaact 
      caatcccgg cctcgggcc tcaccatgt ggtacagcc 
      ttgacaaga agaacaaag cgactcgtc aaggagtca 
      tcaaaatcc gaatcgcct tcagtgcgg aaaaaaacc 
      gacaagtgg ccgcacaag agcacagac acatgttac 
      gcatcattc ttttgcacg aactcgaac tatcaaaag 
      ttgcccagt tctggtggt gtggcactt ttagccccc 
      aaggcgaag cgttgctga cccagcaca tagcccagt 
      ctgggctaa caccaccgg aagtggctt tgtgccaat 
      ggtcggttt acacttccg tccatcaga accaccagt 
      taagtttca gtctgtttt cgaaacttt tggagaaat 
      tggcgaaac cgcttcgga gtgaagaag atttgaaga 
      tattttggg acggggcga agaaattgc taccgtcgt 
      gtttctccg tcattcgga agatcagga agagggatt 
      ttaaggata aaatgacga tgcaacaac tgactgaag 
      ttttgaata ttttgggga atattaaaa tgatggaaa 
      tgccagctg atgttgact tgttcccga acaatatgt 
      atccatggg tgactttta gtgaagaca cgaaacgtg 
      atattttga cggttgttg ctgatgctg ttcagcaag 
      gggccgatg attggttta atcatatca ccgagaata 
      atgttcaac aagttgaaa atgatgtgg gaagattgc 
      agtacttga tccaagcca ttcagttgg tcaaaagcg 
      atctgcaaa aggcgctgg agttttacg acaaactct 
      tccaacaaa caataaact tccaatacg ccaaagcct 
      tatacgtct tctaccaag cgaaaatat cagcccttg 
      ccgaacctg acgtccgca acatttgca aatccctaa 
      agaagcttt ctttcaccg gcttcacaa caaacgaaa 
      ccgactcca acgaaccgc taaaagaag gaacaacct 
      tgttcgaac tgtatttag ctctccacc gatttgttg 
      ttcttggca aaggtttat gtcagacag attcggatt 
      catttcata ttcggaatt tttatcaat ggtttcatg 
      gcggtgtgg ctcaatggc tcgatattg ccgtcttca 
      aagccctcc aacgtattg agaaagccg tagagttgg 
      ataaattag ttcctgtgg acaccactg ttaggtaca 
      gctcttctg ccgtagaca caatctcga ttttttacc 
      aagtgaaag tattttggc aacagctca actggccgg 
      acgttgaag gttgttaca cgttcattg ccaaaataa 
      tagatgata tatgccgtt gttgcgttt tctacgccg 
      aaaggatgg caatgaggg tcgacggaa tgggtgttc 
      aaacagacg agaagaaat ttgaagtga caaatgagt 
      ggtgtttgg ccataaata acatcgaat atgtgagac 
      agtcccttc aacctttta ccgatgaac ttggcatgg 
      aaggagtta ttgaaaaac tggccgatt tgaaaagtg 
      caatggaag caaaacgat gccaagata cgctgaata 
      ctgtcatag caaatgcta ttgataccg tcaaaaacg 
      aaatcatcg cgcttttgg aaactgtgg ttaaaaaga 
      tggtgccag caatgaaac gacttctga tagaaggcg 
      ctgagttgt ttaatcagg atagtaaca gtattgata 
      gactcatgt tatacgtag acagtaatt tatcgactt 
      tacataacg agctaaacg aggagaatt ttaaccgag 
      tcttggaaa tagtgtggg ataatttag acggaattt 
      tgaaggaaa taattcaat caaacttgg agaaacgaa 
      gaccgccgt ctttcttcg ccaatcttc acaaaacat 
      taaacttaa tgcttggct cgtttaaag ccaatgatt 
      taaattgtg aagaactga agaaaaccg aactgaaat 
      tgaaagtga atgggcttg aaaccaacg acttgatcc 
      acttggttc atctgaatt tgtacgaag aattcaaaa 
      atttgaaag agtcgaaat acggggtct tgtccgtaa 
      aagccaaat tcgaaaaca acaacaacc tcaaagtga 
      ctgtaatga acgcacgaa acttattag cgatggatt 
      cgaacggtt atagcgact cgtttgtga ggattcatc 
      tattaccag aacataaat ttagtggga tccctaaac 
      ccaaaacgc aaactcatt acaagactt tgtttcctt 
      tgtatgatg agaacttta ccgtggagc tacccactg 
      attacaaaa gtgtagaag atggactgt tacttttta 
      gtgtcaaag acaaagaca tgttgggtt ataataacc 
      aatacatag gtgaagcgt ttctgcatt ttaaagaca 
      tagattcgt caaatactc caatggacg acttacccc 
      aaatttcgt tagctttag gaaggccta ctgatttaa 
      gtactgaag caataaagg ctctagaaa gtagactag 
      gtgataaac aagccaagg aatttttaa gaaaactta 
      aacaaagaa tgggaacat cataactta agactgtat 
      tgaaaaaaa gtatattga gcgtatttt tacttactg 
      tacttacaa aatatatcg ttattattt gtattgttt 
      tcttttgtt cctttgtga aaatttaac cttttttaa 
      taaatgaac caaatttac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY