Status
Please wait ...
Gene information
General information
TC011255 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 716967..741214
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC011255
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02625

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands irregular - Number of animals showing the phenotype: 4 (41 dpi)
    Days post injection


    abdominal odoriferous glands partially content not present - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection

    abdominal odoriferous glands shape irregular - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011255 RA
      MLCFRIQQV DGGFFEKFG NLLREHAET TENRESEEK 
RKVEQIAEC LRSDECAST DTEFVASSG DSDNEAKSL 
SSDSEDEKD KTDLISAAF NFSLNGNVP RAKPRNNFR 
GVKKDELRR QTSMYLASM TIDELYAEI LYEILHNVG 
CDVSVEIGQ TALISYAQD AFKIPNNKH NQLLSEAEK 
KEAPEFLIN VEVIEAKDL KPKDSNGLS DPFVTLYLA 
SNTSHRYNT SVKYGTLVP TWEEHFALP ISDNSIDDT 
LCIEVWDFD PAESVKEKF GRFFDVKGV KGMRKFVKE 
IAVAAATGQ HENELIGVA KIPLNSIPA SGLTMWYSL 
DKKNKATRQ GVIKIRIAF SAEKNRQVA AQEHRHMLR 
IILLHELEL SKVAQFWWC GTFSPQGEA LLTQHIAQS 
GLTPPEVAL CQWSVYTSV HQNHQLSFS LFSKLLEKL 
AKPLRSEED LKIFWDGAK KLLPSCFSV IRKIRKRDF 
KDKMTMQQL TEVLNILGN IKMMEMPAD VDLFPNNMY 
PWVTFSEDT KRDILTVVA DAVQQGADD WFNHITENN 
VQQVENDVG RLQYLIQAI QLVKSDLQK ALEFYDKLF 
QQTINFQYA KALYVFYQA KISALAEPD VRNICKSLK 
KLSFTGFTT NETDSNEPL KEGTTLFEL YLALHRFVV 
LGKGLCQTD SDSFHIRNF YQWFHGGVA QWLDIAVFK 
ALQRIEKAV ELDKLVPVD TTVRYSSSA VDTISIFYQ 
VKVFWQQLN WPDVEGCYT FIAKIIDDI CRCCVFYAE 
RMAMRVDGM GVQTDEKKF EVTNEWCLA INNIEYVRQ 
SLQPFTDEL GMEGVIEKL ADLKSAMEA KRCQDTLNT 
VIANAIDTV KNEIIALLE TVVKKMVPA MKRLLIEGA 
ELFNQDSNS IDRLMLYVD SNLSTLHNE LNEENFNRV 
LEIVWDNLD GILKEIIQS NLEKRRPPS FFANLHKTL 
NLMLGSFKA NDLNCEELK KTELKLKVN GLETNDLIH 
LVHLNLYEE FKNLKESKY GVLSVKAKF ENNNNLKVT 
VMNARNLLA MDSNGYSDS FVRIHLLPE HKFSGIPKP 
KTQTHYKTL FPLYDENFT VELPTDYKS VEDGLLLFS 
VKDKDMLGY NNQYIGEAF LHFKDIDSS NTPMDDLPQ 
ISLALGRPT DLSTEAIKA LESRLGDKQ AKEFLRKLK 
QRMGTS
    CDS sequence
    • >TC011255 RA
      atgctgtgt ttcagaatt cagcaagtt gacggtggt 
tttttcgag aaattcggc aacttattg cgtgagcat 
gccgaaacg acggaaaac cgggaaagc gaagaaaaa 
cgaaaagtt gaacaaatc gctgaatgt ctccgatct 
gatgaatgc gccagcact gacactgaa ttcgttgct 
tcttctggt gatagcgac aatgaagcc aaaagtttg 
tcttcagac tcagaagac gaaaaggac aagactgat 
ttaatttcc gctgcgttt aattttagc ttgaacgga 
aacgttccg cgcgcaaaa cctcgaaat aatttcaga 
ggtgttaaa aaagatgaa ttgcggcga caaacgagc 
atgtatttg gcgtccatg acaatcgat gagctgtat 
gctgaaatt ttgtacgaa atcttacat aatgttggc 
tgtgatgta agtgttgag ataggacag actgcctta 
atctcgtac gcacaagat gcatttaaa attcctaac 
aataaacat aatcaacta ttgtctgaa gctgaaaag 
aaggaggct cctgaattt ttgatcaat gtcgaagtt 
atcgaagcc aaagatttg aaaccaaag gattccaac 
gggttgagc gatccgttc gttacttta tacttagct 
agtaataca tcacataga tataacact tcggtcaag 
tatgggact ctggtgcca acgtgggaa gaacacttt 
gctttaccg atctctgac aattcaatc gacgacacc 
ctctgcatc gaggtgtgg gacttcgac cccgccgaa 
tccgtcaaa gaaaaattc ggtcgtttc ttcgacgtg 
aaaggtgtc aaaggcatg cgaaaattc gtcaaagag 
atcgcagtg gccgctgcc accggccaa cacgaaaac 
gaactaatc ggcgtcgcc aaaatcccc cttaactca 
atcccggcc tcgggcctc accatgtgg tacagcctt 
gacaagaag aacaaagcg actcgtcaa ggagtcatc 
aaaatccga atcgccttc agtgcggaa aaaaaccga 
caagtggcc gcacaagag cacagacac atgttacgc 
atcattctt ttgcacgaa ctcgaacta tcaaaagtt 
gcccagttc tggtggtgt ggcactttt agcccccaa 
ggcgaagcg ttgctgacc cagcacata gcccagtct 
gggctaaca ccaccggaa gtggctttg tgccaatgg 
tcggtttac acttccgtc catcagaac caccagtta 
agtttcagt ctgttttcg aaacttttg gagaaattg 
gcgaaaccg cttcggagt gaagaagat ttgaagata 
ttttgggac ggggcgaag aaattgcta ccgtcgtgt 
ttctccgtc attcggaag atcaggaag agggatttt 
aaggataaa atgacgatg caacaactg actgaagtt 
ttgaatatt ttggggaat attaaaatg atggaaatg 
ccagctgat gttgacttg ttcccgaac aatatgtat 
ccatgggtg acttttagt gaagacacg aaacgtgat 
attttgacg gttgttgct gatgctgtt cagcaaggg 
gccgatgat tggtttaat catatcacc gagaataat 
gttcaacaa gttgaaaat gatgtggga agattgcag 
tacttgatc caagccatt cagttggtc aaaagcgat 
ctgcaaaag gcgctggag ttttacgac aaactcttc 
caacaaaca ataaacttc caatacgcc aaagcctta 
tacgtcttc taccaagcg aaaatatca gcccttgcc 
gaacctgac gtccgcaac atttgcaaa tccctaaag 
aagctttct ttcaccggc ttcacaaca aacgaaacc 
gactccaac gaaccgcta aaagaagga acaaccttg 
ttcgaactg tatttagct ctccaccga tttgttgtt 
cttggcaaa ggtttatgt cagacagat tcggattca 
tttcatatt cggaatttt tatcaatgg tttcatggc 
ggtgtggct caatggctc gatattgcc gtcttcaaa 
gccctccaa cgtattgag aaagccgta gagttggat 
aaattagtt cctgtggac accactgtt aggtacagc 
tcttctgcc gtagacaca atctcgatt ttttaccaa 
gtgaaagta ttttggcaa cagctcaac tggccggac 
gttgaaggt tgttacacg ttcattgcc aaaataata 
gatgatata tgccgttgt tgcgttttc tacgccgaa 
aggatggca atgagggtc gacggaatg ggtgttcaa 
acagacgag aagaaattt gaagtgaca aatgagtgg 
tgtttggcc ataaataac atcgaatat gtgagacag 
tcccttcaa ccttttacc gatgaactt ggcatggaa 
ggagttatt gaaaaactg gccgatttg aaaagtgca 
atggaagca aaacgatgc caagatacg ctgaatact 
gtcatagca aatgctatt gataccgtc aaaaacgaa 
atcatcgcg cttttggaa actgtggtt aaaaagatg 
gtgccagca atgaaacga cttctgata gaaggcgct 
gagttgttt aatcaggat agtaacagt attgataga 
ctcatgtta tacgtagac agtaattta tcgacttta 
cataacgag ctaaacgag gagaatttt aaccgagtc 
ttggaaata gtgtgggat aatttagac ggaattttg 
aaggaaata attcaatca aacttggag aaacgaaga 
ccgccgtct ttcttcgcc aatcttcac aaaacatta 
aacttaatg cttggctcg tttaaagcc aatgattta 
aattgtgaa gaactgaag aaaaccgaa ctgaaattg 
aaagtgaat gggcttgaa accaacgac ttgatccac 
ttggttcat ctgaatttg tacgaagaa ttcaaaaat 
ttgaaagag tcgaaatac ggggtcttg tccgtaaaa 
gccaaattc gaaaacaac aacaacctc aaagtgact 
gtaatgaac gcacgaaac ttattagcg atggattcg 
aacggttat agcgactcg tttgtgagg attcatcta 
ttaccagaa cataaattt agtgggatc cctaaaccc 
aaaacgcaa actcattac aagactttg tttcctttg 
tatgatgag aactttacc gtggagcta cccactgat 
tacaaaagt gtagaagat ggactgtta ctttttagt 
gtcaaagac aaagacatg ttgggttat aataaccaa 
tacataggt gaagcgttt ctgcatttt aaagacata 
gattcgtca aatactcca atggacgac ttaccccaa 
atttcgtta gctttagga aggcctact gatttaagt 
actgaagca ataaaggct ctagaaagt agactaggt 
gataaacaa gccaaggaa tttttaaga aaacttaaa 
caaagaatg ggaacatca taa
    mRNA sequence
    • >TC011255 RA
      atttgtgaa aaaactgct taatctaaa tagcggtaa 
taatgctgt gtttcagaa ttcagcaag ttgacggtg 
gttttttcg agaaattcg gcaacttat tgcgtgagc 
atgccgaaa cgacggaaa accgggaaa gcgaagaaa 
aacgaaaag ttgaacaaa tcgctgaat gtctccgat 
ctgatgaat gcgccagca ctgacactg aattcgttg 
cttcttctg gtgatagcg acaatgaag ccaaaagtt 
tgtcttcag actcagaag acgaaaagg acaagactg 
atttaattt ccgctgcgt ttaatttta gcttgaacg 
gaaacgttc cgcgcgcaa aacctcgaa ataatttca 
gaggtgtta aaaaagatg aattgcggc gacaaacga 
gcatgtatt tggcgtcca tgacaatcg atgagctgt 
atgctgaaa ttttgtacg aaatcttac ataatgttg 
gctgtgatg taagtgttg agataggac agactgcct 
taatctcgt acgcacaag atgcattta aaattccta 
acaataaac ataatcaac tattgtctg aagctgaaa 
agaaggagg ctcctgaat ttttgatca atgtcgaag 
ttatcgaag ccaaagatt tgaaaccaa aggattcca 
acgggttga gcgatccgt tcgttactt tatacttag 
ctagtaata catcacata gatataaca cttcggtca 
agtatggga ctctggtgc caacgtggg aagaacact 
ttgctttac cgatctctg acaattcaa tcgacgaca 
ccctctgca tcgaggtgt gggacttcg accccgccg 
aatccgtca aagaaaaat tcggtcgtt tcttcgacg 
tgaaaggtg tcaaaggca tgcgaaaat tcgtcaaag 
agatcgcag tggccgctg ccaccggcc aacacgaaa 
acgaactaa tcggcgtcg ccaaaatcc cccttaact 
caatcccgg cctcgggcc tcaccatgt ggtacagcc 
ttgacaaga agaacaaag cgactcgtc aaggagtca 
tcaaaatcc gaatcgcct tcagtgcgg aaaaaaacc 
gacaagtgg ccgcacaag agcacagac acatgttac 
gcatcattc ttttgcacg aactcgaac tatcaaaag 
ttgcccagt tctggtggt gtggcactt ttagccccc 
aaggcgaag cgttgctga cccagcaca tagcccagt 
ctgggctaa caccaccgg aagtggctt tgtgccaat 
ggtcggttt acacttccg tccatcaga accaccagt 
taagtttca gtctgtttt cgaaacttt tggagaaat 
tggcgaaac cgcttcgga gtgaagaag atttgaaga 
tattttggg acggggcga agaaattgc taccgtcgt 
gtttctccg tcattcgga agatcagga agagggatt 
ttaaggata aaatgacga tgcaacaac tgactgaag 
ttttgaata ttttgggga atattaaaa tgatggaaa 
tgccagctg atgttgact tgttcccga acaatatgt 
atccatggg tgactttta gtgaagaca cgaaacgtg 
atattttga cggttgttg ctgatgctg ttcagcaag 
gggccgatg attggttta atcatatca ccgagaata 
atgttcaac aagttgaaa atgatgtgg gaagattgc 
agtacttga tccaagcca ttcagttgg tcaaaagcg 
atctgcaaa aggcgctgg agttttacg acaaactct 
tccaacaaa caataaact tccaatacg ccaaagcct 
tatacgtct tctaccaag cgaaaatat cagcccttg 
ccgaacctg acgtccgca acatttgca aatccctaa 
agaagcttt ctttcaccg gcttcacaa caaacgaaa 
ccgactcca acgaaccgc taaaagaag gaacaacct 
tgttcgaac tgtatttag ctctccacc gatttgttg 
ttcttggca aaggtttat gtcagacag attcggatt 
catttcata ttcggaatt tttatcaat ggtttcatg 
gcggtgtgg ctcaatggc tcgatattg ccgtcttca 
aagccctcc aacgtattg agaaagccg tagagttgg 
ataaattag ttcctgtgg acaccactg ttaggtaca 
gctcttctg ccgtagaca caatctcga ttttttacc 
aagtgaaag tattttggc aacagctca actggccgg 
acgttgaag gttgttaca cgttcattg ccaaaataa 
tagatgata tatgccgtt gttgcgttt tctacgccg 
aaaggatgg caatgaggg tcgacggaa tgggtgttc 
aaacagacg agaagaaat ttgaagtga caaatgagt 
ggtgtttgg ccataaata acatcgaat atgtgagac 
agtcccttc aacctttta ccgatgaac ttggcatgg 
aaggagtta ttgaaaaac tggccgatt tgaaaagtg 
caatggaag caaaacgat gccaagata cgctgaata 
ctgtcatag caaatgcta ttgataccg tcaaaaacg 
aaatcatcg cgcttttgg aaactgtgg ttaaaaaga 
tggtgccag caatgaaac gacttctga tagaaggcg 
ctgagttgt ttaatcagg atagtaaca gtattgata 
gactcatgt tatacgtag acagtaatt tatcgactt 
tacataacg agctaaacg aggagaatt ttaaccgag 
tcttggaaa tagtgtggg ataatttag acggaattt 
tgaaggaaa taattcaat caaacttgg agaaacgaa 
gaccgccgt ctttcttcg ccaatcttc acaaaacat 
taaacttaa tgcttggct cgtttaaag ccaatgatt 
taaattgtg aagaactga agaaaaccg aactgaaat 
tgaaagtga atgggcttg aaaccaacg acttgatcc 
acttggttc atctgaatt tgtacgaag aattcaaaa 
atttgaaag agtcgaaat acggggtct tgtccgtaa 
aagccaaat tcgaaaaca acaacaacc tcaaagtga 
ctgtaatga acgcacgaa acttattag cgatggatt 
cgaacggtt atagcgact cgtttgtga ggattcatc 
tattaccag aacataaat ttagtggga tccctaaac 
ccaaaacgc aaactcatt acaagactt tgtttcctt 
tgtatgatg agaacttta ccgtggagc tacccactg 
attacaaaa gtgtagaag atggactgt tacttttta 
gtgtcaaag acaaagaca tgttgggtt ataataacc 
aatacatag gtgaagcgt ttctgcatt ttaaagaca 
tagattcgt caaatactc caatggacg acttacccc 
aaatttcgt tagctttag gaaggccta ctgatttaa 
gtactgaag caataaagg ctctagaaa gtagactag 
gtgataaac aagccaagg aatttttaa gaaaactta 
aacaaagaa tgggaacat cataactta agactgtat 
tgaaaaaaa gtatattga gcgtatttt tacttactg 
tacttacaa aatatatcg ttattattt gtattgttt 
tcttttgtt cctttgtga aaatttaac cttttttaa 
taaatgaac caaatttac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY