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Gene information
General information
TC030782 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid49: 3319729..3321869
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030782
    iBeetle screen iB_02624
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030782 RA
      MGLVGIVKF FTRRTFELL IIALLLVFL PKIPPEAKF 
      EKPYKVAPP RPFEGALKL NEKLNDAEI WHKGDLHGP 
      EAFVDYNGE LYTSLHGGD VVKLTGNHI TPVVKFGKP 
      CKGIYEERI CGRPLGMAF DKNGVLFVA DSYYGVFKV 
      DVKTGKKER LVAFDEQID GRNVTLPNS VAVASNGDI 
      FWTDSSTEF DLQDGVFDL LADGSGRLI HYDSKTKKN 
      KVLISDLHF ANGVVLSDD EEFVLVGET VRSRIHRYY 
      LKGPKKGTH DIFIEGLPG LVDNLKHDG KGGFIVPLV 
      VAVDNEHPL LTQIMGPFP LLRKFVARI LGLVQFGFK 
      FIGDIYPNE FSKRGVHFV GHFSMASFM YPPRVTILH 
      LSKNGEIKD SLHCLNKKL SGISEAHIF KDTLYLGSP 
      FNDYIGRIP LSKIGWEHL KPTPAPKPT TTTPKPTTT 
      TTTTPKPTT TTTTTTPKP TTTTPKPTT TTPKPTTTT 
      PKATPPTKP AQKEAQAKA VKDQPKAVP VKESPKKN
    CDS sequence
    • >TC030782 RA
      atgggtctt gtcggtatc gtgaagttt tttactcgt 
      agaactttt gaattacta ataatcgct ttattgctt 
      gtgtttctc ccgaaaatc ccccccgag gcaaagttc 
      gaaaaaccg tacaaagtt gcaccgccc cggccgttc 
      gagggggcg cttaaactc aatgagaag ctaaacgat 
      gcagagatt tggcataag ggggacctc cacgggcct 
      gaggctttc gtggattac aacggggag ttgtatacg 
      tcactgcat gggggcgac gttgttaaa ttaacagga 
      aaccacatt acccctgtt gtaaagttt gggaaacct 
      tgtaagggg atctatgag gaacggatc tgtgggcgc 
      cccttaggc atggcgttt gataagaat ggagtctta 
      tttgtggcc gattcgtac tacggggtg ttcaaagtt 
      gacgtcaaa accggcaaa aaggagcga ttggttgcc 
      tttgacgaa cagatcgac ggccggaat gtgactctc 
      ccaaattcc gtggcggtt gcaagcaat ggtgatatt 
      ttctggacg gattcaagc acagagttt gatttacaa 
      gatggtgtt ttcgacttg ttggcggac gggagtggg 
      agattaatt cattatgat tcaaaaacg aaaaaaaat 
      aaagtttta atctcagat ttgcatttt gcaaacggt 
      gttgtctta tccgacgat gaggaattc gttttagtt 
      ggtgaaaca gttcgtagt cgtattcac cgttactat 
      ctgaaaggg cccaagaag ggcacccat gatattttc 
      attgagggg ctcccgggc ctcgttgac aacttgaag 
      cacgacgga aagggcggt ttcattgtc cctttggtt 
      gtagccgtc gataacgaa cacccacta ctcactcaa 
      atcatgggt cctttcccc ctgttacga aaattcgta 
      gctcgaatt ttgggtttg gtccagttc gggttcaaa 
      tttatcggc gatatttat ccgaacgaa ttttcaaaa 
      cgcggcgtg catttcgta ggccatttc agcatggct 
      tcgttcatg tacccccca agggtcacg attttacac 
      ctctccaaa aacggcgag atcaaagac agtttgcac 
      tgtctcaat aaaaaattg tcgggaatt agcgaagcg 
      catatcttc aaagacacg ctttacctt gggtcgcct 
      tttaacgat tacatcggg cgcattcca ttgtcgaaa 
      ataggttgg gagcacttg aaaccaacc cctgctccc 
      aaacctaca actacaaca cctaaaccc accactact 
      actacgaca acacctaaa cctactacc actacaacc 
      acaacaacg cccaaacca accaccaca acacctaaa 
      ccaacgaca acgacgcct aaacctaca accacaaca 
      cccaaagca acccctccg acaaaacct gctcaaaaa 
      gaagctcaa gcaaaagcg gtaaaagac caacccaag 
      gcagtgcca gttaaagaa tcacccaaa aagaattaa
    mRNA sequence
    • >TC030782 RA
      tgttttcag gtgttgtca aaacggttc aaggctggt 
      tcaaggttc gtaaaacgt gaaatgcac agttagcct 
      caagtttta atcgccgtg tcatcattg tagcgttaa 
      gtaacgaaa gatgggtct tgtcggtat cgtgaagtt 
      ttttactcg tagaacttt tgaattact aataatcgc 
      tttattgct tgtgtttct cccgaaaat cccccccga 
      ggcaaagtt cgaaaaacc gtacaaagt tgcaccgcc 
      ccggccgtt cgagggggc gcttaaact caatgagaa 
      gctaaacga tgcagagat ttggcataa gggggacct 
      ccacgggcc tgaggcttt cgtggatta caacgggga 
      gttgtatac gtcactgca tgggggcga cgttgttaa 
      attaacagg aaaccacat tacccctgt tgtaaagtt 
      tgggaaacc ttgtaaggg gatctatga ggaacggat 
      ctgtgggcg ccccttagg catggcgtt tgataagaa 
      tggagtctt atttgtggc cgattcgta ctacggggt 
      gttcaaagt tgacgtcaa aaccggcaa aaaggagcg 
      attggttgc ctttgacga acagatcga cggccggaa 
      tgtgactct cccaaattc cgtggcggt tgcaagcaa 
      tggtgatat tttctggac ggattcaag cacagagtt 
      tgatttaca agatggtgt tttcgactt gttggcgga 
      cgggagtgg gagattaat tcattatga ttcaaaaac 
      gaaaaaaaa taaagtttt aatctcaga tttgcattt 
      tgcaaacgg tgttgtctt atccgacga tgaggaatt 
      cgttttagt tggtgaaac agttcgtag tcgtattca 
      ccgttacta tctgaaagg gcccaagaa gggcaccca 
      tgatatttt cattgaggg gctcccggg cctcgttga 
      caacttgaa gcacgacgg aaagggcgg tttcattgt 
      ccctttggt tgtagccgt cgataacga acacccact 
      actcactca aatcatggg tcctttccc cctgttacg 
      aaaattcgt agctcgaat tttgggttt ggtccagtt 
      cgggttcaa atttatcgg cgatattta tccgaacga 
      attttcaaa acgcggcgt gcatttcgt aggccattt 
      cagcatggc ttcgttcat gtacccccc aagggtcac 
      gattttaca cctctccaa aaacggcga gatcaaaga 
      cagtttgca ctgtctcaa taaaaaatt gtcgggaat 
      tagcgaagc gcatatctt caaagacac gctttacct 
      tgggtcgcc ttttaacga ttacatcgg gcgcattcc 
      attgtcgaa aataggttg ggagcactt gaaaccaac 
      ccctgctcc caaacctac aactacaac acctaaacc 
      caccactac tactacgac aacacctaa acctactac 
      cactacaac cacaacaac gcccaaacc aaccaccac 
      aacacctaa accaacgac aacgacgcc taaacctac 
      aaccacaac acccaaagc aacccctcc gacaaaacc 
      tgctcaaaa agaagctca agcaaaagc ggtaaaaga 
      ccaacccaa ggcagtgcc agttaaaga atcacccaa 
      aaagaatta atctctagt aagctgtga tgttccagt 
      tgttaaccg ttttatatc gtcgcggtc gtcagttgt 
      tttttataa tttttattt aatagactg tataaaata 
      agaaagcca ttccattaa tcaagaatt ctccttttt 
      tattcatgt actgatatt atattttgt agaaatcta 
      gtttttgtt aagttctaa tttgatggc aggattatt 
      atttggtga tactttaca tagttttta tgtatctta 
      ataatcatt ttgtattgg gttctcttt gttggatgt 
      gatgtacct actatttaa ataaaactg tttactaca 
      a