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Gene information
General information
TC030766 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 3296708..3302801
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC030766
    iBeetle screen iB_02622

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Fertility
    eggs lost during procedure: potentially due to defects in egg-shells egg-morphology or egg-size, (14 dpi)
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20
    only four eggs
    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
     legs shortened - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    legs podomer potentially fused - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030766 RA
      MSYPSSRQI PWGVNFLQK LTDRCCPRL HFNRELCYR 
GSVVFLTYV AYMSYHLSR KPISVVKAV LHQNCSGLT 
PPPDVIPGN RSHWCDWAP FNGSDASAS QLLGELDSS 
FLFCYAIAM FISGFIAER VNLRYFLSL GMLMSGIFS 
YLFGIAKSY NIHNLSYYI IVQGLAGVA QTTGWPGVV 
TVMSNWFGK KKRGLIFGL WNSHTSIGN ILGSLIAAE 
YVETDWSLS FIMPGLIVG VVGFVLFLF LVVNPSDVG 
CTVAEPEER TQNGRQYRK LQDRVANVD NVSHVSCET 
DDTDIILGE EEVQRRLSE RTRLLPGNE TSPRDNAIG 
FLGACRIPG VLEYSLSLF FSKLVSYTF LYWLPLYVK 
ESLKMGATL SADLSTLFD VGGIVGAIA AGVISDHSG 
MSATTCVGM LAAAAPMMF IYQKLSYYS LALNMVLLI 
VVGILVNGP YALITTAVS AELGTHSSL EGNSKALAT 
VTAIIDGTG SIGAAVGPL LAGFVSNYG WSNVFIMLM 
ISDILALIL LLRLVKHEI HRCRSVRRE MRIE
    CDS sequence
    • >TC030766 RA
      atgagttat ccctcttcg agacaaatc ccttggggc 
gtgaatttt ttacaaaaa ctgaccgac agatgctgt 
ccaaggctt catttcaac agagaatta tgctaccga 
ggtagtgtt gtctttctt acttatgtg gcctatatg 
agttaccac ttgtctcgg aaaccaatt tcggtagtt 
aaggctgtt ttacatcaa aactgtagc gggctaacg 
cctccgcca gatgtaatt cctgggaat agatcgcat 
tggtgcgat tgggctcct ttcaatggg tctgatgct 
agtgcgtcc cagctattg ggggagtta gattcctcg 
tttttattc tgctacgca attgccatg tttatttcg 
ggatttatt gccgaacga gtcaatctc cgatacttc 
ctgagttta ggcatgcta atgtcaggc atttttagc 
tatctgttt ggtatcgcc aaaagttat aatatacat 
aatttaagt tattacata atagtacaa ggccttgcc 
ggtgttgcc caaacaact ggttggcct ggcgtcgtt 
accgtgatg agtaattgg tttgggaaa aaaaaacgc 
ggactgatc ttcggtcta tggaattcg cacacaagt 
attggcaat attttgggg tctttaata gcagcagag 
tacgtcgaa actgactgg tcgctatcg ttcataatg 
ccaggtttg attgttggg gttgtagga tttgtttta 
tttttattt ttggtggtg aatcctagc gatgttgga 
tgtactgtt gctgaaccg gaagaacga acgcaaaat 
gggcggcaa tatagaaaa ttgcaagat cgggtcgca 
aacgttgat aatgtgtcg catgtcagt tgtgaaacc 
gacgatact gatattatt ttaggtgaa gaggaagta 
cagaggcga ctgtctgaa cggactcgt ctattgccc 
ggcaatgag acttcgccc agagacaac gctattggt 
tttctagga gcctgtcgt attcccggc gttttagaa 
tattcactt tcgttgttt ttttcaaaa ctcgtcagt 
tataccttc ttgtactgg ttgccattg tacgtaaaa 
gagtcactg aaaatgggc gctacactt agtgccgat 
ttgtcaact cttttcgat gttggaggc attgtgggg 
gccatagca gcgggggtc ataagcgac catagcgga 
atgtcagcc acaacgtgt gtggggatg ttggcggcg 
gcggcccca atgatgttc atttatcaa aaattgagc 
tactacagc ttggcttta aacatggta ttactgata 
gtggtcgga atcctagtg aatggccct tacgcccta 
attacaact gccgtttcg gccgaatta ggcacacat 
tccagcctt gaaggtaat tcaaaggcc ttggctaca 
gtgaccgct attatcgac ggaactggt tccatcggg 
gcagctgtt ggtccacta ttggctggc tttgtctcc 
aactatgga tggtccaat gtattcatc atgttgatg 
atctctgat atcttagct ttaattttg ttactacga 
ttagttaaa cacgaaatc cacagatgt cggagtgta 
agaagagaa atgcgtatt gaataa
    mRNA sequence
    • >TC030766 RA
      taccacaca aacatctgt catatttat tactcttgc 
cactgactc actaaactt gacgttttc attgtttta 
aatttccgt aatacaaac gtaaattat caatttaac 
aacaatgat ccacgatta agataacaa cagcttatc 
ttcaatttt atgtttcat tattgcgtg gcaagacta 
gcctttgtt acaacatat cagttttcc atctgttac 
cgtaaatgg aaaatgagt tatccctct tcgagacaa 
atcccttgg ggcgtgaat tttttacaa aaactgacc 
gacagatgc tgtccaagg cttcatttc aacagagaa 
ttatgctac cgaggtagt gttgtcttt cttacttat 
gtggcctat atgagttac cacttgtct cggaaacca 
atttcggta gttaaggct gttttacat caaaactgt 
agcgggcta acgcctccg ccagatgta attcctggg 
aatagatcg cattggtgc gattgggct cctttcaat 
gggtctgat gctagtgcg tcccagcta ttgggggag 
ttagattcc tcgttttta ttctgctac gcaattgcc 
atgtttatt tcgggattt attgccgaa cgagtcaat 
ctccgatac ttcctgagt ttaggcatg ctaatgtca 
ggcattttt agctatctg tttggtatc gccaaaagt 
tataatata cataattta agttattac ataatagta 
caaggcctt gccggtgtt gcccaaaca actggttgg 
cctggcgtc gttaccgtg atgagtaat tggtttggg 
aaaaaaaaa cgcggactg atcttcggt ctatggaat 
tcgcacaca agtattggc aatattttg gggtcttta 
atagcagca gagtacgtc gaaactgac tggtcgcta 
tcgttcata atgccaggt ttgattgtt ggggttgta 
ggatttgtt ttattttta tttttggtg gtgaatcct 
agcgatgtt ggatgtact gttgctgaa ccggaagaa 
cgaacgcaa aatgggcgg caatataga aaattgcaa 
gatcgggtc gcaaacgtt gataatgtg tcgcatgtc 
agttgtgaa accgacgat actgatatt attttaggt 
gaagaggaa gtacagagg cgactgtct gaacggact 
cgtctattg cccggcaat gagacttcg cccagagac 
aacgctatt ggttttcta ggagcctgt cgtattccc 
ggcgtttta gaatattca ctttcgttg tttttttca 
aaactcgtc agttatacc ttcttgtac tggttgcca 
ttgtacgta aaagagtca ctgaaaatg ggcgctaca 
cttagtgcc gatttgtca actcttttc gatgttgga 
ggcattgtg ggggccata gcagcgggg gtcataagc 
gaccatagc ggaatgtca gccacaacg tgtgtgggg 
atgttggcg gcggcggcc ccaatgatg ttcatttat 
caaaaattg agctactac agcttggct ttaaacatg 
gtattactg atagtggtc ggaatccta gtgaatggc 
ccttacgcc ctaattaca actgccgtt tcggccgaa 
ttaggcaca cattccagc cttgaaggt aattcaaag 
gccttggct acagtgacc gctattatc gacggaact 
ggttccatc ggggcagct gttggtcca ctattggct 
ggctttgtc tccaactat ggatggtcc aatgtattc 
atcatgttg atgatctct gatatctta gctttaatt 
ttgttacta cgattagtt aaacacgaa atccacaga 
tgtcggagt gtaagaaga gaaatgcgt attgaataa 
cactaagtt agtgttgtt atgtttgga tgtaacaac 
acaaataga ttaatttta tttaaatta ttaaatttt 
attgtgata taatagttg caattttat gtcgtaaaa 
atatacgga acttccatt aaagttgac aattgaaat 
aaatagtgt cttaataag aacctggtc aagagtgcg 
ttgcaaagt tatgacatt ggacaaaat agtgattag 
ctgtttcaa aactataaa aatgtcaac gtcgcattt 
gctctaaat aaattattc atacactt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY