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Gene information
General information
TC030755 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid46: 1852534..1860576
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030755
    iBeetle screen iB_02621
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 3
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature slightly thinner - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva cuticle potentially thinner - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva bristle thinner - Penetrance (percentage of animals/eggs on slide): less than 30%

    Comment: Bristles are only visible in higher magnifications
    larva seta thinner - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva cuticle thinner - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030755 RA
      MRVSRLKYV ICIYHTVRC ACPIGDDEK HKTIAKQSK 
      GVTLTSDSM EVGPLTSEP TWQQLQDYY NKNASKLVI 
      KDLFAQDPN RFNNFSLKL KTPNDGDIL VDYSKNRIN 
      SDVLKLLFS LARARKVEN ARDAMFSGQ KINFTEDRA 
      VLHTALRNR SNQAILVDG KNVMPEVNA VLEHMKQFT 
      NSVLSGQWK GYTGKPITD VVNIGIGGS DLGPLMVTE 
      ALKAYGRGL RMHFVSNID GTHLAEVLK KIDAESVLF 
      IIASKTFTT QETITNATS AKQWFLTHA KDPAAVAKH 
      FVALSTNGP KVKEFGIDT ANMFEFWDW VGGRYSLWS 
      AIGLSISLA IGFENFLQL LEGAHYMDN HFRSAPLEQ 
      NAPVILALL GVWYHNFYG AETHALLPY DQYLHRFAA 
      YFQQGDMES NGKYVTRSG RTVNYSTGP IVWGEPGTN 
      GQHAFYQLI HQGTRVIPA DFIAPVQTL NPINNGLHH 
      KILLANFLA QTEALMTGK TGEQARAEL QKQGLGGEA 
      LEKILPHKI FEGNRPTNS IMVKKITPF TLGALIAMY 
      EHKIFTQGI IWNINSFDQ WGVELGKQL AKAIEPELQ 
      DNKAVTSHD SSTNGLISF IKANH
    CDS sequence
    • >TC030755 RA
      atgagagtt tctcgtctg aagtatgta atttgtatt 
      tatcacacc gtgcgctgc gcgtgccca atcggcgac 
      gatgaaaaa cacaaaacg atcgccaaa caatcgaaa 
      ggcgtgact ttgacatct gacagcatg gaagtcgga 
      cccttgact tcggagcca acttggcaa caactccaa 
      gattattac aacaagaac gcctcaaag ctggtgatt 
      aaggacctt ttcgcccag gacccgaac cgcttcaac 
      aacttcagc ttgaagctg aaaacgccc aacgatggc 
      gacatcctg gtcgattat tcgaaaaac cgcatcaat 
      tccgatgtt ctcaaactg ttgttttcc ttggcccgc 
      gcccgcaag gtcgaaaat gcacgcgat gcaatgttt 
      tccgggcag aaaatcaac tttacagag gacagggcc 
      gtactgcac acggctttg cgtaatcgc tccaatcag 
      gcgattcta gtcgatggg aaaaatgtg atgccggag 
      gtgaatgcg gtcctggag cacatgaag cagttcact 
      aattcggtc ctgagcggg cagtggaag ggttacaca 
      ggcaagccc atcaccgac gtggtgaat attggcatc 
      gggggctcc gacctgggc ccccttatg gtgaccgaa 
      gccctgaag gcgtacggt cgcggcttg cgaatgcat 
      ttcgtgtcg aacatcgac ggtacgcat ttagccgaa 
      gttttgaaa aaaatcgac gctgagtct gtacttttt 
      atcattgct tcgaaaaca ttcacaaca caggagaca 
      ataaccaat gccacttcg gccaagcag tggttctta 
      acccatgct aaagatcct gctgctgtt gctaaacat 
      ttcgttgct ttgagcaca aatgggccg aaagtgaag 
      gagtttggc atcgatacg gccaatatg tttgagttt 
      tgggactgg gttggaggt aggtattcc ctgtggtcc 
      gctatcggg cttagtatt tcgctggcg attgggttc 
      gagaatttt ctccagttg ttggaaggg gcgcattat 
      atggataac cacttcagg tcggcgccc ttggaacag 
      aacgctcca gtgattttg gcccttttg ggcgtctgg 
      taccacaac ttctacggc gccgagacg cacgctctc 
      ttgccctac gaccagtat ttgcaccgt ttcgcggcc 
      tatttccag cagggtgac atggagagc aacggcaaa 
      tacgtgacc cgaagcggc cgaacggtg aattacagc 
      acagggccg atcgtgtgg ggggagccg gggaccaac 
      gggcagcac gccttctac cagttaatt catcaaggg 
      accagagtt atccccgcc gatttcata gctccggtg 
      caaacgctc aaccccatc aataatgga ctccaccat 
      aaaatattg ctggccaac ttcttggcg caaacggag 
      gcgcttatg acggggaaa acgggggag caggcgcgt 
      gccgagctc cagaagcag gggctgggg ggagaggcc 
      ctggagaag atcctcccg cataagatc ttcgaaggg 
      aacaggccg actaacagt attatggtg aagaaaatc 
      acgccgttt acgctcggc gcgctcatt gccatgtac 
      gagcacaag atcttcact cagggaatc atctggaat 
      atcaattcg tttgaccaa tggggagtg gagctgggg 
      aagcagctg gcgaaggca atcgagccc gaattgcaa 
      gacaataaa gccgtgact tcgcacgac tcgtccaca 
      aacggactc atcagcttc atcaaagcc aatcattaa
    mRNA sequence
    • >TC030755 RA
      gtttaatta ataatttgc gatgagagt ttctcgtct 
      gaagtatgt aatttgtat ttatcacac cgtgcgctg 
      cgcgtgccc aatcggcga cgatgaaaa acacaaaac 
      gatcgccaa acaatcgaa aggcgtgac tttgacatc 
      tgacagcat ggaagtcgg acccttgac ttcggagcc 
      aacttggca acaactcca agattatta caacaagaa 
      cgcctcaaa gctggtgat taaggacct tttcgccca 
      ggacccgaa ccgcttcaa caacttcag cttgaagct 
      gaaaacgcc caacgatgg cgacatcct ggtcgatta 
      ttcgaaaaa ccgcatcaa ttccgatgt tctcaaact 
      gttgttttc cttggcccg cgcccgcaa ggtcgaaaa 
      tgcacgcga tgcaatgtt ttccgggca gaaaatcaa 
      ctttacaga ggacagggc cgtactgca cacggcttt 
      gcgtaatcg ctccaatca ggcgattct agtcgatgg 
      gaaaaatgt gatgccgga ggtgaatgc ggtcctgga 
      gcacatgaa gcagttcac taattcggt cctgagcgg 
      gcagtggaa gggttacac aggcaagcc catcaccga 
      cgtggtgaa tattggcat cgggggctc cgacctggg 
      cccccttat ggtgaccga agccctgaa ggcgtacgg 
      tcgcggctt gcgaatgca tttcgtgtc gaacatcga 
      cggtacgca tttagccga agttttgaa aaaaatcga 
      cgctgagtc tgtactttt tatcattgc ttcgaaaac 
      attcacaac acaggagac aataaccaa tgccacttc 
      ggccaagca gtggttctt aacccatgc taaagatcc 
      tgctgctgt tgctaaaca tttcgttgc tttgagcac 
      aaatgggcc gaaagtgaa ggagtttgg catcgatac 
      ggccaatat gtttgagtt ttgggactg ggttggagg 
      taggtattc cctgtggtc cgctatcgg gcttagtat 
      ttcgctggc gattgggtt cgagaattt tctccagtt 
      gttggaagg ggcgcatta tatggataa ccacttcag 
      gtcggcgcc cttggaaca gaacgctcc agtgatttt 
      ggccctttt gggcgtctg gtaccacaa cttctacgg 
      cgccgagac gcacgctct cttgcccta cgaccagta 
      tttgcaccg tttcgcggc ctatttcca gcagggtga 
      catggagag caacggcaa atacgtgac ccgaagcgg 
      ccgaacggt gaattacag cacagggcc gatcgtgtg 
      gggggagcc ggggaccaa cgggcagca cgccttcta 
      ccagttaat tcatcaagg gaccagagt tatccccgc 
      cgatttcat agctccggt gcaaacgct caaccccat 
      caataatgg actccacca taaaatatt gctggccaa 
      cttcttggc gcaaacgga ggcgcttat gacggggaa 
      aacggggga gcaggcgcg tgccgagct ccagaagca 
      ggggctggg gggagaggc cctggagaa gatcctccc 
      gcataagat cttcgaagg gaacaggcc gactaacag 
      tattatggt gaagaaaat cacgccgtt tacgctcgg 
      cgcgctcat tgccatgta cgagcacaa gatcttcac 
      tcagggaat catctggaa tatcaattc gtttgacca 
      atggggagt ggagctggg gaagcagct ggcgaaggc 
      aatcgagcc cgaattgca agacaataa agccgtgac 
      ttcgcacga ctcgtccac aaacggact catcagctt 
      catcaaagc caatcatta aggggtggc gtggctctg 
      tataggtgt tagggagat taatatatt gtatgtttt 
      tattaataa agagatatt agaagt