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Gene information
General information
TC030755 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 1972735..1980777
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC030755
    iBeetle screen iB_02621

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 3
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature slightly thinner - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva cuticle potentially thinner - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva bristle thinner - Penetrance (percentage of animals/eggs on slide): less than 30%

    Comment: Bristles are only visible in higher magnifications
    larva seta thinner - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva cuticle thinner - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030755 RA
      MRVSRLKYV ICIYHTVRC ACPIGDDEK HKTIAKQSK 
GVTLTSDSM EVGPLTSEP TWQQLQDYY NKNASKLVI 
KDLFAQDPN RFNNFSLKL KTPNDGDIL VDYSKNRIN 
SDVLKLLFS LARARKVEN ARDAMFSGQ KINFTEDRA 
VLHTALRNR SNQAILVDG KNVMPEVNA VLEHMKQFT 
NSVLSGQWK GYTGKPITD VVNIGIGGS DLGPLMVTE 
ALKAYGRGL RMHFVSNID GTHLAEVLK KIDAESVLF 
IIASKTFTT QETITNATS AKQWFLTHA KDPAAVAKH 
FVALSTNGP KVKEFGIDT ANMFEFWDW VGGRYSLWS 
AIGLSISLA IGFENFLQL LEGAHYMDN HFRSAPLEQ 
NAPVILALL GVWYHNFYG AETHALLPY DQYLHRFAA 
YFQQGDMES NGKYVTRSG RTVNYSTGP IVWGEPGTN 
GQHAFYQLI HQGTRVIPA DFIAPVQTL NPINNGLHH 
KILLANFLA QTEALMTGK TGEQARAEL QKQGLGGEA 
LEKILPHKI FEGNRPTNS IMVKKITPF TLGALIAMY 
EHKIFTQGI IWNINSFDQ WGVELGKQL AKAIEPELQ 
DNKAVTSHD SSTNGLISF IKANH
    CDS sequence
    • >TC030755 RA
      atgagagtt tctcgtctg aagtatgta atttgtatt 
tatcacacc gtgcgctgc gcgtgccca atcggcgac 
gatgaaaaa cacaaaacg atcgccaaa caatcgaaa 
ggcgtgact ttgacatct gacagcatg gaagtcgga 
cccttgact tcggagcca acttggcaa caactccaa 
gattattac aacaagaac gcctcaaag ctggtgatt 
aaggacctt ttcgcccag gacccgaac cgcttcaac 
aacttcagc ttgaagctg aaaacgccc aacgatggc 
gacatcctg gtcgattat tcgaaaaac cgcatcaat 
tccgatgtt ctcaaactg ttgttttcc ttggcccgc 
gcccgcaag gtcgaaaat gcacgcgat gcaatgttt 
tccgggcag aaaatcaac tttacagag gacagggcc 
gtactgcac acggctttg cgtaatcgc tccaatcag 
gcgattcta gtcgatggg aaaaatgtg atgccggag 
gtgaatgcg gtcctggag cacatgaag cagttcact 
aattcggtc ctgagcggg cagtggaag ggttacaca 
ggcaagccc atcaccgac gtggtgaat attggcatc 
gggggctcc gacctgggc ccccttatg gtgaccgaa 
gccctgaag gcgtacggt cgcggcttg cgaatgcat 
ttcgtgtcg aacatcgac ggtacgcat ttagccgaa 
gttttgaaa aaaatcgac gctgagtct gtacttttt 
atcattgct tcgaaaaca ttcacaaca caggagaca 
ataaccaat gccacttcg gccaagcag tggttctta 
acccatgct aaagatcct gctgctgtt gctaaacat 
ttcgttgct ttgagcaca aatgggccg aaagtgaag 
gagtttggc atcgatacg gccaatatg tttgagttt 
tgggactgg gttggaggt aggtattcc ctgtggtcc 
gctatcggg cttagtatt tcgctggcg attgggttc 
gagaatttt ctccagttg ttggaaggg gcgcattat 
atggataac cacttcagg tcggcgccc ttggaacag 
aacgctcca gtgattttg gcccttttg ggcgtctgg 
taccacaac ttctacggc gccgagacg cacgctctc 
ttgccctac gaccagtat ttgcaccgt ttcgcggcc 
tatttccag cagggtgac atggagagc aacggcaaa 
tacgtgacc cgaagcggc cgaacggtg aattacagc 
acagggccg atcgtgtgg ggggagccg gggaccaac 
gggcagcac gccttctac cagttaatt catcaaggg 
accagagtt atccccgcc gatttcata gctccggtg 
caaacgctc aaccccatc aataatgga ctccaccat 
aaaatattg ctggccaac ttcttggcg caaacggag 
gcgcttatg acggggaaa acgggggag caggcgcgt 
gccgagctc cagaagcag gggctgggg ggagaggcc 
ctggagaag atcctcccg cataagatc ttcgaaggg 
aacaggccg actaacagt attatggtg aagaaaatc 
acgccgttt acgctcggc gcgctcatt gccatgtac 
gagcacaag atcttcact cagggaatc atctggaat 
atcaattcg tttgaccaa tggggagtg gagctgggg 
aagcagctg gcgaaggca atcgagccc gaattgcaa 
gacaataaa gccgtgact tcgcacgac tcgtccaca 
aacggactc atcagcttc atcaaagcc aatcattaa
    mRNA sequence
    • >TC030755 RA
      gtttaatta ataatttgc gatgagagt ttctcgtct 
gaagtatgt aatttgtat ttatcacac cgtgcgctg 
cgcgtgccc aatcggcga cgatgaaaa acacaaaac 
gatcgccaa acaatcgaa aggcgtgac tttgacatc 
tgacagcat ggaagtcgg acccttgac ttcggagcc 
aacttggca acaactcca agattatta caacaagaa 
cgcctcaaa gctggtgat taaggacct tttcgccca 
ggacccgaa ccgcttcaa caacttcag cttgaagct 
gaaaacgcc caacgatgg cgacatcct ggtcgatta 
ttcgaaaaa ccgcatcaa ttccgatgt tctcaaact 
gttgttttc cttggcccg cgcccgcaa ggtcgaaaa 
tgcacgcga tgcaatgtt ttccgggca gaaaatcaa 
ctttacaga ggacagggc cgtactgca cacggcttt 
gcgtaatcg ctccaatca ggcgattct agtcgatgg 
gaaaaatgt gatgccgga ggtgaatgc ggtcctgga 
gcacatgaa gcagttcac taattcggt cctgagcgg 
gcagtggaa gggttacac aggcaagcc catcaccga 
cgtggtgaa tattggcat cgggggctc cgacctggg 
cccccttat ggtgaccga agccctgaa ggcgtacgg 
tcgcggctt gcgaatgca tttcgtgtc gaacatcga 
cggtacgca tttagccga agttttgaa aaaaatcga 
cgctgagtc tgtactttt tatcattgc ttcgaaaac 
attcacaac acaggagac aataaccaa tgccacttc 
ggccaagca gtggttctt aacccatgc taaagatcc 
tgctgctgt tgctaaaca tttcgttgc tttgagcac 
aaatgggcc gaaagtgaa ggagtttgg catcgatac 
ggccaatat gtttgagtt ttgggactg ggttggagg 
taggtattc cctgtggtc cgctatcgg gcttagtat 
ttcgctggc gattgggtt cgagaattt tctccagtt 
gttggaagg ggcgcatta tatggataa ccacttcag 
gtcggcgcc cttggaaca gaacgctcc agtgatttt 
ggccctttt gggcgtctg gtaccacaa cttctacgg 
cgccgagac gcacgctct cttgcccta cgaccagta 
tttgcaccg tttcgcggc ctatttcca gcagggtga 
catggagag caacggcaa atacgtgac ccgaagcgg 
ccgaacggt gaattacag cacagggcc gatcgtgtg 
gggggagcc ggggaccaa cgggcagca cgccttcta 
ccagttaat tcatcaagg gaccagagt tatccccgc 
cgatttcat agctccggt gcaaacgct caaccccat 
caataatgg actccacca taaaatatt gctggccaa 
cttcttggc gcaaacgga ggcgcttat gacggggaa 
aacggggga gcaggcgcg tgccgagct ccagaagca 
ggggctggg gggagaggc cctggagaa gatcctccc 
gcataagat cttcgaagg gaacaggcc gactaacag 
tattatggt gaagaaaat cacgccgtt tacgctcgg 
cgcgctcat tgccatgta cgagcacaa gatcttcac 
tcagggaat catctggaa tatcaattc gtttgacca 
atggggagt ggagctggg gaagcagct ggcgaaggc 
aatcgagcc cgaattgca agacaataa agccgtgac 
ttcgcacga ctcgtccac aaacggact catcagctt 
catcaaagc caatcatta aggggtggc gtggctctg 
tataggtgt tagggagat taatatatt gtatgtttt 
tattaataa agagatatt agaagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY