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Gene information
General information
TC030725 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 7503692..7516463
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030725
    iBeetle screen iB_02618

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Fertility
    vitellogenic egg chamber late mostlynot present - Number of animals showing the phenotype: 2

    egg chamber number decreased - Number of animals showing the phenotype: 2
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030725 RA
      MHSLTLEGQ YTNTNGAAS TEKVVILDA GAQYGKLID 
      RKVRELNVE SHILPLDTP AFNIKEGGY KAVIISGGP 
      SSVYAEDAP RYDGDIFRI GLPVLGICY GMQMMNKEF 
      GGSVIRKDS REDGQYNIE VNTKCILFK GLDKVQTVL 
      LTHGDSIDK VAENFRPIA QSSSFCAGI CNEKLRLYG 
      LQFHPEVDL TPNGKTILK NFLFDVAGL TGTYTMTSR 
      EKECLKYVK NAVGNNKVL LLVSGGVDS TVAAALLHK 
      ALRPDQIIA IHIDNGFMR KNESSKVYQ SLQKIGMNL 
      RVVKAASTF MEGTTVVPV DKHEATLRT TLTSMLCKT 
      CDPEEKRKI IGDVFMRVS DEIISDLNL KPEEVVLAQ 
      GTLRPDLIE SASALVSSK ADTIKTHHN DSELVRKLR 
      SEGRVVEPL KDFHKDEVR ALGRDLGLT PELVMRHPF 
      PGPGLAIRV LCAEEPYME RDFSETQVL VKIIVEFDQ 
      MLQKKHALL NRVEAVTNN EERNVLRKI SSKQKLAAT 
      LLPIRSVGV QGDCRSYSY VVGISSEKE PDWDDLMFL 
      ACLIPRICR NINRVCYVF GGLVKEPIL DITITYLTS 
      NVLATLRQC DDVATTVLQ NSGQMNSVS QMPVILLPL 
      HFDRDQALR IPSCQRSIV LRPFCTRDF MTGMPAVPG 
      KQLPLDVVQ KMVVEILQV PGVSRVLYD LTSKPPGTT 
      EWE
    CDS sequence
    • >TC030725 RA
      atgcacagt ctaacactg gaaggtcag tacacgaac 
      acgaatggg gctgccagc acggagaag gtcgtcatc 
      ttggacgcg ggggcccag tacggcaaa ctcatcgac 
      agaaaggtg agggagctg aacgtcgag agccacatc 
      ttgccgctg gacacgcca gcgttcaac atcaaggaa 
      gggggctac aaggcggtc atcatctcc ggggggccc 
      agctcggtc tacgcggag gacgcgccg cggtacgac 
      ggcgatatc tttcggata gggctgccg gtcttaggg 
      atttgctat gggatgcag atgatgaat aaggaattt 
      gggggctcc gttattaga aaagacagc cgcgaagac 
      ggacaatat aatatcgaa gtcaacacg aaatgcatt 
      ttgtttaaa ggcttggac aaggtccag acagtcctc 
      ctcacccac ggcgactcg atcgacaaa gtcgccgag 
      aatttccga ccgatcgcc caaagctcc tccttctgt 
      gccggcatc tgcaacgag aaactccgc ctttacggt 
      ctccagttc caccccgag gtcgacctc acccccaac 
      ggcaaaacc atcctcaaa aatttcctc ttcgacgtc 
      gccggtctc accggcacc tacaccatg accagtcgc 
      gaaaaggaa tgtctcaaa tacgtgaaa aacgccgtc 
      ggaaacaac aaagtcctg cttttggtc agcggaggc 
      gtcgacagc actgtcgcc gccgccctt ctccacaag 
      gctctgcgc cccgatcaa atcatcgca attcacata 
      gacaacggc ttcatgcgc aagaacgaa agctcgaaa 
      gtttaccaa tcgctgcaa aagatcgga atgaacttg 
      cgagtggtc aaagcagcg agcactttc atggaaggt 
      acgacggtc gtaccagtc gataaacac gaagccacc 
      ttgcgaaca actctgact agcatgctg tgcaagacg 
      tgcgacccg gaggaaaaa cgcaaaata atcggggac 
      gtgtttatg cgagtctcc gatgaaata atctccgat 
      ttgaatttg aaaccggag gaagtggtt ttggcccaa 
      gggaccctc aggccggac ctgatcgag tcggcgtcg 
      gccctggtt tcgagcaaa gccgacacg attaaaacc 
      caccacaac gattcggag ctggtgcgg aagttgcgt 
      tccgaaggg cgtgtggtt gagccgctg aaggacttc 
      cacaaggat gaagtccgg gcgctggga cgtgattta 
      ggtttaacg ccggaattg gtgatgagg catccgttt 
      ccggggccc ggattagca atccgggtg ttgtgtgcc 
      gaagagcct tatatggag cgtgatttc agcgagacg 
      caagttcta gttaagatt atcgttgag tttgaccag 
      atgttgcag aagaaacac gcactgttg aatagggtg 
      gaggcggtg acgaataat gaagaaagg aatgtgttg 
      cggaagatt tcgagtaag cagaagttg gcggcgaca 
      ctgttgccg attagaagt gtgggagta cagggtgat 
      tgtcgatcg tatagttac gtcgtggga atatcgagt 
      gaaaaagaa ccggattgg gacgatttg atgtttttg 
      gcttgtctc ataccgagg atttgtcga aatattaat 
      cgggtttgt tatgtattt ggaggactg gttaaagaa 
      cccattttg gatattacc atcacgtat ctgacgagt 
      aatgtgttg gcaacgttg aggcagtgt gacgacgtt 
      gccacaaca gtgttacag aacagtggt caaatgaac 
      tcagtaagt caaatgcct gtgattctg ctaccttta 
      cacttcgac agggaccag gcattgagg atcccttct 
      tgtcaaagg tcgatagtg ttgcgaccc ttctgcacg 
      cgtgatttt atgacggga atgccagcc gtacctgga 
      aaacaactg cctttagat gtcgtgcag aagatggtc 
      gtagagata ttacaagtc cctggtgtt tctagagta 
      ctttacgat ttaacatca aagccacca ggcacaaca 
      gaatgggaa tga
    mRNA sequence
    • >TC030725 RA
      tcaaacagt tcgctgcgt tctttttgt ccataaaag 
      tgatgtgat tgacatttt ctatcaaca aatttcatt 
      cctgattat tgttattta ataattaca tctctgagt 
      gctgcggtc gcaaagcta tgcacagtc taacactgg 
      aaggtcagt acacgaaca cgaatgggg ctgccagca 
      cggagaagg tcgtcatct tggacgcgg gggcccagt 
      acggcaaac tcatcgaca gaaaggtga gggagctga 
      acgtcgaga gccacatct tgccgctgg acacgccag 
      cgttcaaca tcaaggaag ggggctaca aggcggtca 
      tcatctccg gggggccca gctcggtct acgcggagg 
      acgcgccgc ggtacgacg gcgatatct ttcggatag 
      ggctgccgg tcttaggga tttgctatg ggatgcaga 
      tgatgaata aggaatttg ggggctccg ttattagaa 
      aagacagcc gcgaagacg gacaatata atatcgaag 
      tcaacacga aatgcattt tgtttaaag gcttggaca 
      aggtccaga cagtcctcc tcacccacg gcgactcga 
      tcgacaaag tcgccgaga atttccgac cgatcgccc 
      aaagctcct ccttctgtg ccggcatct gcaacgaga 
      aactccgcc tttacggtc tccagttcc accccgagg 
      tcgacctca cccccaacg gcaaaacca tcctcaaaa 
      atttcctct tcgacgtcg ccggtctca ccggcacct 
      acaccatga ccagtcgcg aaaaggaat gtctcaaat 
      acgtgaaaa acgccgtcg gaaacaaca aagtcctgc 
      ttttggtca gcggaggcg tcgacagca ctgtcgccg 
      ccgcccttc tccacaagg ctctgcgcc ccgatcaaa 
      tcatcgcaa ttcacatag acaacggct tcatgcgca 
      agaacgaaa gctcgaaag tttaccaat cgctgcaaa 
      agatcggaa tgaacttgc gagtggtca aagcagcga 
      gcactttca tggaaggta cgacggtcg taccagtcg 
      ataaacacg aagccacct tgcgaacaa ctctgacta 
      gcatgctgt gcaagacgt gcgacccgg aggaaaaac 
      gcaaaataa tcggggacg tgtttatgc gagtctccg 
      atgaaataa tctccgatt tgaatttga aaccggagg 
      aagtggttt tggcccaag ggaccctca ggccggacc 
      tgatcgagt cggcgtcgg ccctggttt cgagcaaag 
      ccgacacga ttaaaaccc accacaacg attcggagc 
      tggtgcgga agttgcgtt ccgaagggc gtgtggttg 
      agccgctga aggacttcc acaaggatg aagtccggg 
      cgctgggac gtgatttag gtttaacgc cggaattgg 
      tgatgaggc atccgtttc cggggcccg gattagcaa 
      tccgggtgt tgtgtgccg aagagcctt atatggagc 
      gtgatttca gcgagacgc aagttctag ttaagatta 
      tcgttgagt ttgaccaga tgttgcaga agaaacacg 
      cactgttga atagggtgg aggcggtga cgaataatg 
      aagaaagga atgtgttgc ggaagattt cgagtaagc 
      agaagttgg cggcgacac tgttgccga ttagaagtg 
      tgggagtac agggtgatt gtcgatcgt atagttacg 
      tcgtgggaa tatcgagtg aaaaagaac cggattggg 
      acgatttga tgtttttgg cttgtctca taccgagga 
      tttgtcgaa atattaatc gggtttgtt atgtatttg 
      gaggactgg ttaaagaac ccattttgg atattacca 
      tcacgtatc tgacgagta atgtgttgg caacgttga 
      ggcagtgtg acgacgttg ccacaacag tgttacaga 
      acagtggtc aaatgaact cagtaagtc aaatgcctg 
      tgattctgc tacctttac acttcgaca gggaccagg 
      cattgagga tcccttctt gtcaaaggt cgatagtgt 
      tgcgaccct tctgcacgc gtgatttta tgacgggaa 
      tgccagccg tacctggaa aacaactgc ctttagatg 
      tcgtgcaga agatggtcg tagagatat tacaagtcc 
      ctggtgttt ctagagtac tttacgatt taacatcaa 
      agccaccag gcacaacag aatgggaat gataatctt 
      ataaattat taattgtgt aatttgtta gcatggaca 
      gactataag aaggtttcc tgtttttat tctttttaa 
      aaaatatta tatatggtt gttgaaaaa ctaaggcga 
      ctgtgtttt gttacgatt ttatttttt aattcactt 
      ggctcaaaa atattgatt tgtatatgt tgattgtgc 
      acctatttt cagacaaat aataacgga atcatattg 
      ttatgcgaa atatgctac attttttat tattaaagt 
      ggcaagttg cactttgta tattaactt attttattt 
      tttccattg tgttaataa aagtcaatt ctattg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY