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Gene information
General information
TC030714 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 304839..309366
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030714
    iBeetle screen iB_02617
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection



    larva lethality late (L7) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030714 RA
      MYNNNNQYS GQGYQSTYP QYQQSYNSN YGYEGYPQS 
      QYMNTNVQP VPPGEEYPQ NNWAPVGGY HNNVPAQAS 
      STTFDGKNE DEGVAQERK AQNAQLIKQ REEYVRKAR 
      VLRRELELL RNQKHEIMS ENSSQRDME LILKENVKL 
      QDEIQTKMK AIHNVIEML SSIIKDGSK ISDLEAQLD 
      ESPKTHHKN HVKSDSVDP AIKYNYMHY DPELHWCRM 
      CDEFPKTAK DFLLHLQSD KHRNNIQEN DVGDNTPWH 
      KLPPEPELP CYEGAPKKR IPIRGLQFF ISAPAWYCK 
      LCDTWIGDL HCASHHLKS QAHFQNYAN FVEQNPHWE 
      IEWLKDREK AMARNERRG RSSDSDSDK KKKKHRNKR 
      SSMESSSKD KKKKKRSKK RRKHSSDTS SSSSSSDSS 
      SEEEENNDK SKSIRVVMR NMKVQSIMN EDLSGKWEM 
      LERLVDQHK KKEEMQKSK EAQKREQPE DNLINQWMT 
      VSEPQEKEK VLLDNLKDR MKQKQELER AKYAEMEKK 
      QREKEREER ELKERKSRE EQEAKERYE RERRERDRE 
      EYERIRDKD RNQVRFKPK DRENYRKRK TPSPSPERP 
      PEKPPQPPQ QRQSKERRE TPSKEETRE RKNSRENGR 
      ESQERPRSR SRSEERRQN ESKKPSGPP TKRLPFIGR 
      MPLFKNKKV EEKVEKEIK KEDYDLPRV TRFQPGNLA 
      RAFLPQPDV VCFPKLSSY PPISAPPPP SVSQPDPPA 
      PPKISEDKP PKAPPAPKI GSSEIDDKK SPEKEEEMY 
      EDPSQMMMN QYYSDYSAM YSQMYDYSQ CQEEPPQPP 
      PPDDIPLAH TMPLQPPPL PPNDPSEDL AMLGISADD 
      MAAQSF
    • >TC030714 RB
      MVDGCRVND EHLRPARNG KVRVTPKII INKKFFTSL 
      LSEPRYAMY NNNNQYSGQ GYQSTYPQY QQSYNSNYG 
      YEGYPQSQY MNTNVQPVP PGEEYPQNN WAPVGGYHN 
      NVPAQASST TFDGKNEDE GVAQERKAQ NAQLIKQRE 
      EYVRKARVL RRELELLRN QKHEIMSEN SSQRDMELI 
      LKENVKLQD EIQTKMKAI HNVIEMLSS IIKDGSKIS 
      DLEAQLDES PKTHHKNHV KSDSVDPAI KYNYMHYDP 
      ELHWCRMCD EFPKTAKDF LLHLQSDKH RNNIQENDV 
      GDNTPWHKL PPEPELPCY EGAPKKRIP IRGLQFFIS 
      APAWYCKLC DTWIGDLHC ASHHLKSQA HFQNYANFV 
      EQNPHWEIE WLKDREKAM ARNERRGRS SDSDSDKKK 
      KKHRNKRSS MESSSKDKK KKKRSKKRR KHSSDTSSS 
      SSSSDSSSE EEENNDKSK SIRVVMRNM KVQSIMNED 
      LSGKWEMLE RLVDQHKKK EEMQKSKEA QKREQPEDN 
      LINQWMTVS EPQEKEKVL LDNLKDRMK QKQELERAK 
      YAEMEKKQR EKEREEREL KERKSREEQ EAKERYERE 
      RRERDREEY ERIRDKDRN QVRFKPKDR ENYRKRKTP 
      SPSPERPPE KPPQPPQQR QSKERRETP SKEETRERK 
      NSRENGRES QERPRSRSR SEERRQNES KKPSGPPTK 
      RLPFIGRMP LFKNKKVEE KVEKEIKKE DYDLPRVTR 
      FQPGNLARA FLPQPDVVC FPKLSSYPP ISAPPPPSV 
      SQPDPPAPP KISEDKPPK APPAPKIGS SEIDDKKSP 
      EKEEEMYED PSQMMMNQY YSDYSAMYS QMYDYSQCQ 
      EEPPQPPPP DDIPLAHTM PLQPPPLPP NDPSEDLAM 
      LGISADDMA AQSF
    CDS sequence
    • >TC030714 RA
      atgtacaat aataacaat cagtactcg gggcaaggg 
      taccaaagt acctacccc cagtaccag cagagctac 
      aattcaaat tacggatat gaggggtac cctcaaagc 
      cagtacatg aacacgaat gttcagcca gtgcctcca 
      ggtgaggaa tacccgcaa aacaattgg gcaccggtt 
      gggggctac cataacaac gttccagcc caagcttca 
      agcactacc tttgacggc aaaaacgaa gacgaaggt 
      gtggcacaa gaaagaaaa gcacagaac gcccaattg 
      attaaacaa cgtgaagaa tatgtgcga aaagcgcgc 
      gttttacga agagaactt gaacttctt cgcaaccaa 
      aaacatgaa attatgtcg gaaaattcc tcacagcgt 
      gacatggag ttgattttg aaggaaaat gttaaatta 
      caggacgaa attcaaaca aaaatgaaa gcaatacat 
      aatgtcatt gagatgttg tcgagtatt atcaaagac 
      ggttcgaaa atttcggat cttgaagct caactagat 
      gaatcaccg aaaacccat cataaaaat catgtgaaa 
      tccgattcg gttgatcct gcaatcaaa tataattac 
      atgcattac gatccggaa ttgcattgg tgtcgaatg 
      tgtgatgaa tttcctaaa acggccaaa gattttctc 
      ttacattta caaagcgat aaacatcgt aataacata 
      caagagaac gacgttggt gataataca ccatggcat 
      aaattacca ccagaaccg gaacttcct tgctatgaa 
      ggagctccc aagaaaaga attccaatt cgaggattg 
      caatttttt atttcggct ccagcttgg tattgcaaa 
      ctttgcgat acatggatt ggtgattta cattgtgct 
      tcacatcat ttaaaatca caagcccat tttcaaaat 
      tatgcgaat ttcgtcgaa caaaatcca cattgggaa 
      attgagtgg ttaaaagat cgcgaaaaa gcaatggct 
      cgtaacgag agacgtggt cgttcttcc gattcagat 
      tccgataaa aagaagaag aaacatcga aataaaaga 
      tcatcaatg gaatcgagt agtaaagac aagaaaaag 
      aagaaacgt tcaaagaaa cgtaggaag cattccagt 
      gatacgagt agtagttca agttcatcc gattcctca 
      tcggaagaa gaagaaaat aacgataaa tcgaaaagt 
      attcgagtt gtgatgcgt aacatgaaa gtccaatca 
      ataatgaat gaggattta agcggtaaa tgggaaatg 
      ttggagcgt ttagtcgat caacataaa aaaaaggaa 
      gaaatgcaa aaaagtaaa gaagcacaa aagagagaa 
      caaccggaa gataatttg ataaatcaa tggatgaca 
      gtgagcgaa cctcaagaa aaagaaaaa gtcttactt 
      gataattta aaagataga atgaagcaa aagcaagaa 
      ttggaaaga gcaaaatat gccgaaatg gaaaagaaa 
      caacgtgag aaggaacga gaagaacgc gaattaaaa 
      gagagaaaa tcaagggaa gaacaagaa gctaaagaa 
      cgctacgaa agggaacgg cgtgaaaga gatcgagag 
      gagtatgag agaataagg gacaaagat cgcaatcaa 
      gtgaggttc aaaccgaaa gatcgggag aattacagg 
      aagaggaag acgccgtct cctagccct gaaaggccc 
      ccagaaaaa cccccacaa ccaccacaa caaagacaa 
      tcgaaggag aggagagag acaccaagc aaggaggag 
      accagggaa aggaagaac agtcgtgag aacggacga 
      gagagtcaa gagcgcccc agaagtcgc agtcgaagc 
      gaagagcgt cgccagaat gagtcaaag aagccctca 
      ggtccgccc acgaaacgt ttaccgttc attggccga 
      atgccactt ttcaaaaac aagaaagtg gaggaaaaa 
      gtggagaaa gaaatcaag aaggaggat tacgatctt 
      ccgagagtt acccgtttc cagcctgga aacctagca 
      cgtgcattc cttccacaa cccgatgtt gtgtgtttt 
      cccaaactt tcgagttat cccccgatt tcggcacca 
      cctccaccg agtgtctca cagcctgat cctccagca 
      cctccgaaa attagcgaa gataaacca ccgaaagca 
      ccaccagct ccgaaaatt ggatcgagt gaaattgac 
      gataaaaag agtccggag aaagaagag gagatgtat 
      gaggatccg agtcagatg atgatgaat caatattat 
      tccgattat agtgcgatg tattcgcaa atgtatgac 
      tattcacag tgtcaggag gagccgcca caaccgccg 
      ccccctgat gatattcca ttggcgcat accatgccc 
      ttgcaaccg cccccgttg ccgccaaat gaccccagt 
      gaggatttg gcgatgttg ggaattagt gcagacgat 
      atggctgct caaagtttc taa
    • >TC030714 RB
      atggttgat ggttgcaga gtaaatgac gaacacttg 
      cgaccagcg agaaatggc aaagtaagg gttacaccg 
      aaaatcatt atcaacaag aaatttttt acgagtttg 
      ttgtcggag ccacgttac gccatgtac aataataac 
      aatcagtac tcggggcaa gggtaccaa agtacctac 
      ccccagtac cagcagagc tacaattca aattacgga 
      tatgagggg taccctcaa agccagtac atgaacacg 
      aatgttcag ccagtgcct ccaggtgag gaatacccg 
      caaaacaat tgggcaccg gttgggggc taccataac 
      aacgttcca gcccaagct tcaagcact acctttgac 
      ggcaaaaac gaagacgaa ggtgtggca caagaaaga 
      aaagcacag aacgcccaa ttgattaaa caacgtgaa 
      gaatatgtg cgaaaagcg cgcgtttta cgaagagaa 
      cttgaactt cttcgcaac caaaaacat gaaattatg 
      tcggaaaat tcctcacag cgtgacatg gagttgatt 
      ttgaaggaa aatgttaaa ttacaggac gaaattcaa 
      acaaaaatg aaagcaata cataatgtc attgagatg 
      ttgtcgagt attatcaaa gacggttcg aaaatttcg 
      gatcttgaa gctcaacta gatgaatca ccgaaaacc 
      catcataaa aatcatgtg aaatccgat tcggttgat 
      cctgcaatc aaatataat tacatgcat tacgatccg 
      gaattgcat tggtgtcga atgtgtgat gaatttcct 
      aaaacggcc aaagatttt ctcttacat ttacaaagc 
      gataaacat cgtaataac atacaagag aacgacgtt 
      ggtgataat acaccatgg cataaatta ccaccagaa 
      ccggaactt ccttgctat gaaggagct cccaagaaa 
      agaattcca attcgagga ttgcaattt tttatttcg 
      gctccagct tggtattgc aaactttgc gatacatgg 
      attggtgat ttacattgt gcttcacat catttaaaa 
      tcacaagcc cattttcaa aattatgcg aatttcgtc 
      gaacaaaat ccacattgg gaaattgag tggttaaaa 
      gatcgcgaa aaagcaatg gctcgtaac gagagacgt 
      ggtcgttct tccgattca gattccgat aaaaagaag 
      aagaaacat cgaaataaa agatcatca atggaatcg 
      agtagtaaa gacaagaaa aagaagaaa cgttcaaag 
      aaacgtagg aagcattcc agtgatacg agtagtagt 
      tcaagttca tccgattcc tcatcggaa gaagaagaa 
      aataacgat aaatcgaaa agtattcga gttgtgatg 
      cgtaacatg aaagtccaa tcaataatg aatgaggat 
      ttaagcggt aaatgggaa atgttggag cgtttagtc 
      gatcaacat aaaaaaaag gaagaaatg caaaaaagt 
      aaagaagca caaaagaga gaacaaccg gaagataat 
      ttgataaat caatggatg acagtgagc gaacctcaa 
      gaaaaagaa aaagtctta cttgataat ttaaaagat 
      agaatgaag caaaagcaa gaattggaa agagcaaaa 
      tatgccgaa atggaaaag aaacaacgt gagaaggaa 
      cgagaagaa cgcgaatta aaagagaga aaatcaagg 
      gaagaacaa gaagctaaa gaacgctac gaaagggaa 
      cggcgtgaa agagatcga gaggagtat gagagaata 
      agggacaaa gatcgcaat caagtgagg ttcaaaccg 
      aaagatcgg gagaattac aggaagagg aagacgccg 
      tctcctagc cctgaaagg cccccagaa aaaccccca 
      caaccacca caacaaaga caatcgaag gagaggaga 
      gagacacca agcaaggag gagaccagg gaaaggaag 
      aacagtcgt gagaacgga cgagagagt caagagcgc 
      cccagaagt cgcagtcga agcgaagag cgtcgccag 
      aatgagtca aagaagccc tcaggtccg cccacgaaa 
      cgtttaccg ttcattggc cgaatgcca cttttcaaa 
      aacaagaaa gtggaggaa aaagtggag aaagaaatc 
      aagaaggag gattacgat cttccgaga gttacccgt 
      ttccagcct ggaaaccta gcacgtgca ttccttcca 
      caacccgat gttgtgtgt tttcccaaa ctttcgagt 
      tatcccccg atttcggca ccacctcca ccgagtgtc 
      tcacagcct gatcctcca gcacctccg aaaattagc 
      gaagataaa ccaccgaaa gcaccacca gctccgaaa 
      attggatcg agtgaaatt gacgataaa aagagtccg 
      gagaaagaa gaggagatg tatgaggat ccgagtcag 
      atgatgatg aatcaatat tattccgat tatagtgcg 
      atgtattcg caaatgtat gactattca cagtgtcag 
      gaggagccg ccacaaccg ccgccccct gatgatatt 
      ccattggcg cataccatg cccttgcaa ccgcccccg 
      ttgccgcca aatgacccc agtgaggat ttggcgatg 
      ttgggaatt agtgcagac gatatggct gctcaaagt 
      ttctaa
    mRNA sequence
    • >TC030714 RA
      cacaaccac gcaaggtgg agtgagttg tgtttagtg 
      ttttattcg agtaaatga cgaacactt gcgaccagc 
      gagaaatgg caaagtaag ggttacacc gaaaatcat 
      tatcaacaa gaaattttt tacgagttt gttgtcgga 
      gccacgtta cgccatgta caataataa caatcagta 
      ctcggggca agggtacca aagtaccta cccccagta 
      ccagcagag ctacaattc aaattacgg atatgaggg 
      gtaccctca aagccagta catgaacac gaatgttca 
      gccagtgcc tccaggtga ggaataccc gcaaaacaa 
      ttgggcacc ggttggggg ctaccataa caacgttcc 
      agcccaagc ttcaagcac tacctttga cggcaaaaa 
      cgaagacga aggtgtggc acaagaaag aaaagcaca 
      gaacgccca attgattaa acaacgtga agaatatgt 
      gcgaaaagc gcgcgtttt acgaagaga acttgaact 
      tcttcgcaa ccaaaaaca tgaaattat gtcggaaaa 
      ttcctcaca gcgtgacat ggagttgat tttgaagga 
      aaatgttaa attacagga cgaaattca aacaaaaat 
      gaaagcaat acataatgt cattgagat gttgtcgag 
      tattatcaa agacggttc gaaaatttc ggatcttga 
      agctcaact agatgaatc accgaaaac ccatcataa 
      aaatcatgt gaaatccga ttcggttga tcctgcaat 
      caaatataa ttacatgca ttacgatcc ggaattgca 
      ttggtgtcg aatgtgtga tgaatttcc taaaacggc 
      caaagattt tctcttaca tttacaaag cgataaaca 
      tcgtaataa catacaaga gaacgacgt tggtgataa 
      tacaccatg gcataaatt accaccaga accggaact 
      tccttgcta tgaaggagc tcccaagaa aagaattcc 
      aattcgagg attgcaatt ttttatttc ggctccagc 
      ttggtattg caaactttg cgatacatg gattggtga 
      tttacattg tgcttcaca tcatttaaa atcacaagc 
      ccattttca aaattatgc gaatttcgt cgaacaaaa 
      tccacattg ggaaattga gtggttaaa agatcgcga 
      aaaagcaat ggctcgtaa cgagagacg tggtcgttc 
      ttccgattc agattccga taaaaagaa gaagaaaca 
      tcgaaataa aagatcatc aatggaatc gagtagtaa 
      agacaagaa aaagaagaa acgttcaaa gaaacgtag 
      gaagcattc cagtgatac gagtagtag ttcaagttc 
      atccgattc ctcatcgga agaagaaga aaataacga 
      taaatcgaa aagtattcg agttgtgat gcgtaacat 
      gaaagtcca atcaataat gaatgagga tttaagcgg 
      taaatggga aatgttgga gcgtttagt cgatcaaca 
      taaaaaaaa ggaagaaat gcaaaaaag taaagaagc 
      acaaaagag agaacaacc ggaagataa tttgataaa 
      tcaatggat gacagtgag cgaacctca agaaaaaga 
      aaaagtctt acttgataa tttaaaaga tagaatgaa 
      gcaaaagca agaattgga aagagcaaa atatgccga 
      aatggaaaa gaaacaacg tgagaagga acgagaaga 
      acgcgaatt aaaagagag aaaatcaag ggaagaaca 
      agaagctaa agaacgcta cgaaaggga acggcgtga 
      aagagatcg agaggagta tgagagaat aagggacaa 
      agatcgcaa tcaagtgag gttcaaacc gaaagatcg 
      ggagaatta caggaagag gaagacgcc gtctcctag 
      ccctgaaag gcccccaga aaaaccccc acaaccacc 
      acaacaaag acaatcgaa ggagaggag agagacacc 
      aagcaagga ggagaccag ggaaaggaa gaacagtcg 
      tgagaacgg acgagagag tcaagagcg ccccagaag 
      tcgcagtcg aagcgaaga gcgtcgcca gaatgagtc 
      aaagaagcc ctcaggtcc gcccacgaa acgtttacc 
      gttcattgg ccgaatgcc acttttcaa aaacaagaa 
      agtggagga aaaagtgga gaaagaaat caagaagga 
      ggattacga tcttccgag agttacccg tttccagcc 
      tggaaacct agcacgtgc attccttcc acaacccga 
      tgttgtgtg ttttcccaa actttcgag ttatccccc 
      gatttcggc accacctcc accgagtgt ctcacagcc 
      tgatcctcc agcacctcc gaaaattag cgaagataa 
      accaccgaa agcaccacc agctccgaa aattggatc 
      gagtgaaat tgacgataa aaagagtcc ggagaaaga 
      agaggagat gtatgagga tccgagtca gatgatgat 
      gaatcaata ttattccga ttatagtgc gatgtattc 
      gcaaatgta tgactattc acagtgtca ggaggagcc 
      gccacaacc gccgccccc tgatgatat tccattggc 
      gcataccat gcccttgca accgccccc gttgccgcc 
      aaatgaccc cagtgagga tttggcgat gttgggaat 
      tagtgcaga cgatatggc tgctcaaag tttctaagt 
      ttttgatat tagacaatt attttgctg tagtttggc 
      cgattggtc gctattttt aagaccgta atatgttat 
      ttgccttaa taaatgcgt ttcttttgt
    • >TC030714 RB
      cacaaccac gcaaggtgg agtgagttg tgtttagtg 
      ttttattcg atgtgattt attacaatt atggttgat 
      ggttgcaga gtaaatgac gaacacttg cgaccagcg 
      agaaatggc aaagtaagg gttacaccg aaaatcatt 
      atcaacaag aaatttttt acgagtttg ttgtcggag 
      ccacgttac gccatgtac aataataac aatcagtac 
      tcggggcaa gggtaccaa agtacctac ccccagtac 
      cagcagagc tacaattca aattacgga tatgagggg 
      taccctcaa agccagtac atgaacacg aatgttcag 
      ccagtgcct ccaggtgag gaatacccg caaaacaat 
      tgggcaccg gttgggggc taccataac aacgttcca 
      gcccaagct tcaagcact acctttgac ggcaaaaac 
      gaagacgaa ggtgtggca caagaaaga aaagcacag 
      aacgcccaa ttgattaaa caacgtgaa gaatatgtg 
      cgaaaagcg cgcgtttta cgaagagaa cttgaactt 
      cttcgcaac caaaaacat gaaattatg tcggaaaat 
      tcctcacag cgtgacatg gagttgatt ttgaaggaa 
      aatgttaaa ttacaggac gaaattcaa acaaaaatg 
      aaagcaata cataatgtc attgagatg ttgtcgagt 
      attatcaaa gacggttcg aaaatttcg gatcttgaa 
      gctcaacta gatgaatca ccgaaaacc catcataaa 
      aatcatgtg aaatccgat tcggttgat cctgcaatc 
      aaatataat tacatgcat tacgatccg gaattgcat 
      tggtgtcga atgtgtgat gaatttcct aaaacggcc 
      aaagatttt ctcttacat ttacaaagc gataaacat 
      cgtaataac atacaagag aacgacgtt ggtgataat 
      acaccatgg cataaatta ccaccagaa ccggaactt 
      ccttgctat gaaggagct cccaagaaa agaattcca 
      attcgagga ttgcaattt tttatttcg gctccagct 
      tggtattgc aaactttgc gatacatgg attggtgat 
      ttacattgt gcttcacat catttaaaa tcacaagcc 
      cattttcaa aattatgcg aatttcgtc gaacaaaat 
      ccacattgg gaaattgag tggttaaaa gatcgcgaa 
      aaagcaatg gctcgtaac gagagacgt ggtcgttct 
      tccgattca gattccgat aaaaagaag aagaaacat 
      cgaaataaa agatcatca atggaatcg agtagtaaa 
      gacaagaaa aagaagaaa cgttcaaag aaacgtagg 
      aagcattcc agtgatacg agtagtagt tcaagttca 
      tccgattcc tcatcggaa gaagaagaa aataacgat 
      aaatcgaaa agtattcga gttgtgatg cgtaacatg 
      aaagtccaa tcaataatg aatgaggat ttaagcggt 
      aaatgggaa atgttggag cgtttagtc gatcaacat 
      aaaaaaaag gaagaaatg caaaaaagt aaagaagca 
      caaaagaga gaacaaccg gaagataat ttgataaat 
      caatggatg acagtgagc gaacctcaa gaaaaagaa 
      aaagtctta cttgataat ttaaaagat agaatgaag 
      caaaagcaa gaattggaa agagcaaaa tatgccgaa 
      atggaaaag aaacaacgt gagaaggaa cgagaagaa 
      cgcgaatta aaagagaga aaatcaagg gaagaacaa 
      gaagctaaa gaacgctac gaaagggaa cggcgtgaa 
      agagatcga gaggagtat gagagaata agggacaaa 
      gatcgcaat caagtgagg ttcaaaccg aaagatcgg 
      gagaattac aggaagagg aagacgccg tctcctagc 
      cctgaaagg cccccagaa aaaccccca caaccacca 
      caacaaaga caatcgaag gagaggaga gagacacca 
      agcaaggag gagaccagg gaaaggaag aacagtcgt 
      gagaacgga cgagagagt caagagcgc cccagaagt 
      cgcagtcga agcgaagag cgtcgccag aatgagtca 
      aagaagccc tcaggtccg cccacgaaa cgtttaccg 
      ttcattggc cgaatgcca cttttcaaa aacaagaaa 
      gtggaggaa aaagtggag aaagaaatc aagaaggag 
      gattacgat cttccgaga gttacccgt ttccagcct 
      ggaaaccta gcacgtgca ttccttcca caacccgat 
      gttgtgtgt tttcccaaa ctttcgagt tatcccccg 
      atttcggca ccacctcca ccgagtgtc tcacagcct 
      gatcctcca gcacctccg aaaattagc gaagataaa 
      ccaccgaaa gcaccacca gctccgaaa attggatcg 
      agtgaaatt gacgataaa aagagtccg gagaaagaa 
      gaggagatg tatgaggat ccgagtcag atgatgatg 
      aatcaatat tattccgat tatagtgcg atgtattcg 
      caaatgtat gactattca cagtgtcag gaggagccg 
      ccacaaccg ccgccccct gatgatatt ccattggcg 
      cataccatg cccttgcaa ccgcccccg ttgccgcca 
      aatgacccc agtgaggat ttggcgatg ttgggaatt 
      agtgcagac gatatggct gctcaaagt ttctaagtt 
      tttgatatt agacaatta ttttgctgt agtttggcc 
      gattggtcg ctattttta agaccgtaa tatgttatt 
      tgccttaat aaatgcgtt tcttttgt