| General information | |||
| TC030714 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 304839..309366 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC030714 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02617 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 5 (11 dpi)
larva lethality late (L7) - Number of animals showing the phenotype: 2 (16 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
- >TC030714 RA
MYNNNNQYS GQGYQSTYP QYQQSYNSN YGYEGYPQS QYMNTNVQP VPPGEEYPQ NNWAPVGGY HNNVPAQAS STTFDGKNE DEGVAQERK AQNAQLIKQ REEYVRKAR VLRRELELL RNQKHEIMS ENSSQRDME LILKENVKL QDEIQTKMK AIHNVIEML SSIIKDGSK ISDLEAQLD ESPKTHHKN HVKSDSVDP AIKYNYMHY DPELHWCRM CDEFPKTAK DFLLHLQSD KHRNNIQEN DVGDNTPWH KLPPEPELP CYEGAPKKR IPIRGLQFF ISAPAWYCK LCDTWIGDL HCASHHLKS QAHFQNYAN FVEQNPHWE IEWLKDREK AMARNERRG RSSDSDSDK KKKKHRNKR SSMESSSKD KKKKKRSKK RRKHSSDTS SSSSSSDSS SEEEENNDK SKSIRVVMR NMKVQSIMN EDLSGKWEM LERLVDQHK KKEEMQKSK EAQKREQPE DNLINQWMT VSEPQEKEK VLLDNLKDR MKQKQELER AKYAEMEKK QREKEREER ELKERKSRE EQEAKERYE RERRERDRE EYERIRDKD RNQVRFKPK DRENYRKRK TPSPSPERP PEKPPQPPQ QRQSKERRE TPSKEETRE RKNSRENGR ESQERPRSR SRSEERRQN ESKKPSGPP TKRLPFIGR MPLFKNKKV EEKVEKEIK KEDYDLPRV TRFQPGNLA RAFLPQPDV VCFPKLSSY PPISAPPPP SVSQPDPPA PPKISEDKP PKAPPAPKI GSSEIDDKK SPEKEEEMY EDPSQMMMN QYYSDYSAM YSQMYDYSQ CQEEPPQPP PPDDIPLAH TMPLQPPPL PPNDPSEDL AMLGISADD MAAQSF
- >TC030714 RB
MVDGCRVND EHLRPARNG KVRVTPKII INKKFFTSL LSEPRYAMY NNNNQYSGQ GYQSTYPQY QQSYNSNYG YEGYPQSQY MNTNVQPVP PGEEYPQNN WAPVGGYHN NVPAQASST TFDGKNEDE GVAQERKAQ NAQLIKQRE EYVRKARVL RRELELLRN QKHEIMSEN SSQRDMELI LKENVKLQD EIQTKMKAI HNVIEMLSS IIKDGSKIS DLEAQLDES PKTHHKNHV KSDSVDPAI KYNYMHYDP ELHWCRMCD EFPKTAKDF LLHLQSDKH RNNIQENDV GDNTPWHKL PPEPELPCY EGAPKKRIP IRGLQFFIS APAWYCKLC DTWIGDLHC ASHHLKSQA HFQNYANFV EQNPHWEIE WLKDREKAM ARNERRGRS SDSDSDKKK KKHRNKRSS MESSSKDKK KKKRSKKRR KHSSDTSSS SSSSDSSSE EEENNDKSK SIRVVMRNM KVQSIMNED LSGKWEMLE RLVDQHKKK EEMQKSKEA QKREQPEDN LINQWMTVS EPQEKEKVL LDNLKDRMK QKQELERAK YAEMEKKQR EKEREEREL KERKSREEQ EAKERYERE RRERDREEY ERIRDKDRN QVRFKPKDR ENYRKRKTP SPSPERPPE KPPQPPQQR QSKERRETP SKEETRERK NSRENGRES QERPRSRSR SEERRQNES KKPSGPPTK RLPFIGRMP LFKNKKVEE KVEKEIKKE DYDLPRVTR FQPGNLARA FLPQPDVVC FPKLSSYPP ISAPPPPSV SQPDPPAPP KISEDKPPK APPAPKIGS SEIDDKKSP EKEEEMYED PSQMMMNQY YSDYSAMYS QMYDYSQCQ EEPPQPPPP DDIPLAHTM PLQPPPLPP NDPSEDLAM LGISADDMA AQSF
- >TC030714 RA
atgtacaat aataacaat cagtactcg gggcaaggg taccaaagt acctacccc cagtaccag cagagctac aattcaaat tacggatat gaggggtac cctcaaagc cagtacatg aacacgaat gttcagcca gtgcctcca ggtgaggaa tacccgcaa aacaattgg gcaccggtt gggggctac cataacaac gttccagcc caagcttca agcactacc tttgacggc aaaaacgaa gacgaaggt gtggcacaa gaaagaaaa gcacagaac gcccaattg attaaacaa cgtgaagaa tatgtgcga aaagcgcgc gttttacga agagaactt gaacttctt cgcaaccaa aaacatgaa attatgtcg gaaaattcc tcacagcgt gacatggag ttgattttg aaggaaaat gttaaatta caggacgaa attcaaaca aaaatgaaa gcaatacat aatgtcatt gagatgttg tcgagtatt atcaaagac ggttcgaaa atttcggat cttgaagct caactagat gaatcaccg aaaacccat cataaaaat catgtgaaa tccgattcg gttgatcct gcaatcaaa tataattac atgcattac gatccggaa ttgcattgg tgtcgaatg tgtgatgaa tttcctaaa acggccaaa gattttctc ttacattta caaagcgat aaacatcgt aataacata caagagaac gacgttggt gataataca ccatggcat aaattacca ccagaaccg gaacttcct tgctatgaa ggagctccc aagaaaaga attccaatt cgaggattg caatttttt atttcggct ccagcttgg tattgcaaa ctttgcgat acatggatt ggtgattta cattgtgct tcacatcat ttaaaatca caagcccat tttcaaaat tatgcgaat ttcgtcgaa caaaatcca cattgggaa attgagtgg ttaaaagat cgcgaaaaa gcaatggct cgtaacgag agacgtggt cgttcttcc gattcagat tccgataaa aagaagaag aaacatcga aataaaaga tcatcaatg gaatcgagt agtaaagac aagaaaaag aagaaacgt tcaaagaaa cgtaggaag cattccagt gatacgagt agtagttca agttcatcc gattcctca tcggaagaa gaagaaaat aacgataaa tcgaaaagt attcgagtt gtgatgcgt aacatgaaa gtccaatca ataatgaat gaggattta agcggtaaa tgggaaatg ttggagcgt ttagtcgat caacataaa aaaaaggaa gaaatgcaa aaaagtaaa gaagcacaa aagagagaa caaccggaa gataatttg ataaatcaa tggatgaca gtgagcgaa cctcaagaa aaagaaaaa gtcttactt gataattta aaagataga atgaagcaa aagcaagaa ttggaaaga gcaaaatat gccgaaatg gaaaagaaa caacgtgag aaggaacga gaagaacgc gaattaaaa gagagaaaa tcaagggaa gaacaagaa gctaaagaa cgctacgaa agggaacgg cgtgaaaga gatcgagag gagtatgag agaataagg gacaaagat cgcaatcaa gtgaggttc aaaccgaaa gatcgggag aattacagg aagaggaag acgccgtct cctagccct gaaaggccc ccagaaaaa cccccacaa ccaccacaa caaagacaa tcgaaggag aggagagag acaccaagc aaggaggag accagggaa aggaagaac agtcgtgag aacggacga gagagtcaa gagcgcccc agaagtcgc agtcgaagc gaagagcgt cgccagaat gagtcaaag aagccctca ggtccgccc acgaaacgt ttaccgttc attggccga atgccactt ttcaaaaac aagaaagtg gaggaaaaa gtggagaaa gaaatcaag aaggaggat tacgatctt ccgagagtt acccgtttc cagcctgga aacctagca cgtgcattc cttccacaa cccgatgtt gtgtgtttt cccaaactt tcgagttat cccccgatt tcggcacca cctccaccg agtgtctca cagcctgat cctccagca cctccgaaa attagcgaa gataaacca ccgaaagca ccaccagct ccgaaaatt ggatcgagt gaaattgac gataaaaag agtccggag aaagaagag gagatgtat gaggatccg agtcagatg atgatgaat caatattat tccgattat agtgcgatg tattcgcaa atgtatgac tattcacag tgtcaggag gagccgcca caaccgccg ccccctgat gatattcca ttggcgcat accatgccc ttgcaaccg cccccgttg ccgccaaat gaccccagt gaggatttg gcgatgttg ggaattagt gcagacgat atggctgct caaagtttc taa
- >TC030714 RB
atggttgat ggttgcaga gtaaatgac gaacacttg cgaccagcg agaaatggc aaagtaagg gttacaccg aaaatcatt atcaacaag aaatttttt acgagtttg ttgtcggag ccacgttac gccatgtac aataataac aatcagtac tcggggcaa gggtaccaa agtacctac ccccagtac cagcagagc tacaattca aattacgga tatgagggg taccctcaa agccagtac atgaacacg aatgttcag ccagtgcct ccaggtgag gaatacccg caaaacaat tgggcaccg gttgggggc taccataac aacgttcca gcccaagct tcaagcact acctttgac ggcaaaaac gaagacgaa ggtgtggca caagaaaga aaagcacag aacgcccaa ttgattaaa caacgtgaa gaatatgtg cgaaaagcg cgcgtttta cgaagagaa cttgaactt cttcgcaac caaaaacat gaaattatg tcggaaaat tcctcacag cgtgacatg gagttgatt ttgaaggaa aatgttaaa ttacaggac gaaattcaa acaaaaatg aaagcaata cataatgtc attgagatg ttgtcgagt attatcaaa gacggttcg aaaatttcg gatcttgaa gctcaacta gatgaatca ccgaaaacc catcataaa aatcatgtg aaatccgat tcggttgat cctgcaatc aaatataat tacatgcat tacgatccg gaattgcat tggtgtcga atgtgtgat gaatttcct aaaacggcc aaagatttt ctcttacat ttacaaagc gataaacat cgtaataac atacaagag aacgacgtt ggtgataat acaccatgg cataaatta ccaccagaa ccggaactt ccttgctat gaaggagct cccaagaaa agaattcca attcgagga ttgcaattt tttatttcg gctccagct tggtattgc aaactttgc gatacatgg attggtgat ttacattgt gcttcacat catttaaaa tcacaagcc cattttcaa aattatgcg aatttcgtc gaacaaaat ccacattgg gaaattgag tggttaaaa gatcgcgaa aaagcaatg gctcgtaac gagagacgt ggtcgttct tccgattca gattccgat aaaaagaag aagaaacat cgaaataaa agatcatca atggaatcg agtagtaaa gacaagaaa aagaagaaa cgttcaaag aaacgtagg aagcattcc agtgatacg agtagtagt tcaagttca tccgattcc tcatcggaa gaagaagaa aataacgat aaatcgaaa agtattcga gttgtgatg cgtaacatg aaagtccaa tcaataatg aatgaggat ttaagcggt aaatgggaa atgttggag cgtttagtc gatcaacat aaaaaaaag gaagaaatg caaaaaagt aaagaagca caaaagaga gaacaaccg gaagataat ttgataaat caatggatg acagtgagc gaacctcaa gaaaaagaa aaagtctta cttgataat ttaaaagat agaatgaag caaaagcaa gaattggaa agagcaaaa tatgccgaa atggaaaag aaacaacgt gagaaggaa cgagaagaa cgcgaatta aaagagaga aaatcaagg gaagaacaa gaagctaaa gaacgctac gaaagggaa cggcgtgaa agagatcga gaggagtat gagagaata agggacaaa gatcgcaat caagtgagg ttcaaaccg aaagatcgg gagaattac aggaagagg aagacgccg tctcctagc cctgaaagg cccccagaa aaaccccca caaccacca caacaaaga caatcgaag gagaggaga gagacacca agcaaggag gagaccagg gaaaggaag aacagtcgt gagaacgga cgagagagt caagagcgc cccagaagt cgcagtcga agcgaagag cgtcgccag aatgagtca aagaagccc tcaggtccg cccacgaaa cgtttaccg ttcattggc cgaatgcca cttttcaaa aacaagaaa gtggaggaa aaagtggag aaagaaatc aagaaggag gattacgat cttccgaga gttacccgt ttccagcct ggaaaccta gcacgtgca ttccttcca caacccgat gttgtgtgt tttcccaaa ctttcgagt tatcccccg atttcggca ccacctcca ccgagtgtc tcacagcct gatcctcca gcacctccg aaaattagc gaagataaa ccaccgaaa gcaccacca gctccgaaa attggatcg agtgaaatt gacgataaa aagagtccg gagaaagaa gaggagatg tatgaggat ccgagtcag atgatgatg aatcaatat tattccgat tatagtgcg atgtattcg caaatgtat gactattca cagtgtcag gaggagccg ccacaaccg ccgccccct gatgatatt ccattggcg cataccatg cccttgcaa ccgcccccg ttgccgcca aatgacccc agtgaggat ttggcgatg ttgggaatt agtgcagac gatatggct gctcaaagt ttctaa
- >TC030714 RA
cacaaccac gcaaggtgg agtgagttg tgtttagtg ttttattcg agtaaatga cgaacactt gcgaccagc gagaaatgg caaagtaag ggttacacc gaaaatcat tatcaacaa gaaattttt tacgagttt gttgtcgga gccacgtta cgccatgta caataataa caatcagta ctcggggca agggtacca aagtaccta cccccagta ccagcagag ctacaattc aaattacgg atatgaggg gtaccctca aagccagta catgaacac gaatgttca gccagtgcc tccaggtga ggaataccc gcaaaacaa ttgggcacc ggttggggg ctaccataa caacgttcc agcccaagc ttcaagcac tacctttga cggcaaaaa cgaagacga aggtgtggc acaagaaag aaaagcaca gaacgccca attgattaa acaacgtga agaatatgt gcgaaaagc gcgcgtttt acgaagaga acttgaact tcttcgcaa ccaaaaaca tgaaattat gtcggaaaa ttcctcaca gcgtgacat ggagttgat tttgaagga aaatgttaa attacagga cgaaattca aacaaaaat gaaagcaat acataatgt cattgagat gttgtcgag tattatcaa agacggttc gaaaatttc ggatcttga agctcaact agatgaatc accgaaaac ccatcataa aaatcatgt gaaatccga ttcggttga tcctgcaat caaatataa ttacatgca ttacgatcc ggaattgca ttggtgtcg aatgtgtga tgaatttcc taaaacggc caaagattt tctcttaca tttacaaag cgataaaca tcgtaataa catacaaga gaacgacgt tggtgataa tacaccatg gcataaatt accaccaga accggaact tccttgcta tgaaggagc tcccaagaa aagaattcc aattcgagg attgcaatt ttttatttc ggctccagc ttggtattg caaactttg cgatacatg gattggtga tttacattg tgcttcaca tcatttaaa atcacaagc ccattttca aaattatgc gaatttcgt cgaacaaaa tccacattg ggaaattga gtggttaaa agatcgcga aaaagcaat ggctcgtaa cgagagacg tggtcgttc ttccgattc agattccga taaaaagaa gaagaaaca tcgaaataa aagatcatc aatggaatc gagtagtaa agacaagaa aaagaagaa acgttcaaa gaaacgtag gaagcattc cagtgatac gagtagtag ttcaagttc atccgattc ctcatcgga agaagaaga aaataacga taaatcgaa aagtattcg agttgtgat gcgtaacat gaaagtcca atcaataat gaatgagga tttaagcgg taaatggga aatgttgga gcgtttagt cgatcaaca taaaaaaaa ggaagaaat gcaaaaaag taaagaagc acaaaagag agaacaacc ggaagataa tttgataaa tcaatggat gacagtgag cgaacctca agaaaaaga aaaagtctt acttgataa tttaaaaga tagaatgaa gcaaaagca agaattgga aagagcaaa atatgccga aatggaaaa gaaacaacg tgagaagga acgagaaga acgcgaatt aaaagagag aaaatcaag ggaagaaca agaagctaa agaacgcta cgaaaggga acggcgtga aagagatcg agaggagta tgagagaat aagggacaa agatcgcaa tcaagtgag gttcaaacc gaaagatcg ggagaatta caggaagag gaagacgcc gtctcctag ccctgaaag gcccccaga aaaaccccc acaaccacc acaacaaag acaatcgaa ggagaggag agagacacc aagcaagga ggagaccag ggaaaggaa gaacagtcg tgagaacgg acgagagag tcaagagcg ccccagaag tcgcagtcg aagcgaaga gcgtcgcca gaatgagtc aaagaagcc ctcaggtcc gcccacgaa acgtttacc gttcattgg ccgaatgcc acttttcaa aaacaagaa agtggagga aaaagtgga gaaagaaat caagaagga ggattacga tcttccgag agttacccg tttccagcc tggaaacct agcacgtgc attccttcc acaacccga tgttgtgtg ttttcccaa actttcgag ttatccccc gatttcggc accacctcc accgagtgt ctcacagcc tgatcctcc agcacctcc gaaaattag cgaagataa accaccgaa agcaccacc agctccgaa aattggatc gagtgaaat tgacgataa aaagagtcc ggagaaaga agaggagat gtatgagga tccgagtca gatgatgat gaatcaata ttattccga ttatagtgc gatgtattc gcaaatgta tgactattc acagtgtca ggaggagcc gccacaacc gccgccccc tgatgatat tccattggc gcataccat gcccttgca accgccccc gttgccgcc aaatgaccc cagtgagga tttggcgat gttgggaat tagtgcaga cgatatggc tgctcaaag tttctaagt ttttgatat tagacaatt attttgctg tagtttggc cgattggtc gctattttt aagaccgta atatgttat ttgccttaa taaatgcgt ttcttttgt
- >TC030714 RB
cacaaccac gcaaggtgg agtgagttg tgtttagtg ttttattcg atgtgattt attacaatt atggttgat ggttgcaga gtaaatgac gaacacttg cgaccagcg agaaatggc aaagtaagg gttacaccg aaaatcatt atcaacaag aaatttttt acgagtttg ttgtcggag ccacgttac gccatgtac aataataac aatcagtac tcggggcaa gggtaccaa agtacctac ccccagtac cagcagagc tacaattca aattacgga tatgagggg taccctcaa agccagtac atgaacacg aatgttcag ccagtgcct ccaggtgag gaatacccg caaaacaat tgggcaccg gttgggggc taccataac aacgttcca gcccaagct tcaagcact acctttgac ggcaaaaac gaagacgaa ggtgtggca caagaaaga aaagcacag aacgcccaa ttgattaaa caacgtgaa gaatatgtg cgaaaagcg cgcgtttta cgaagagaa cttgaactt cttcgcaac caaaaacat gaaattatg tcggaaaat tcctcacag cgtgacatg gagttgatt ttgaaggaa aatgttaaa ttacaggac gaaattcaa acaaaaatg aaagcaata cataatgtc attgagatg ttgtcgagt attatcaaa gacggttcg aaaatttcg gatcttgaa gctcaacta gatgaatca ccgaaaacc catcataaa aatcatgtg aaatccgat tcggttgat cctgcaatc aaatataat tacatgcat tacgatccg gaattgcat tggtgtcga atgtgtgat gaatttcct aaaacggcc aaagatttt ctcttacat ttacaaagc gataaacat cgtaataac atacaagag aacgacgtt ggtgataat acaccatgg cataaatta ccaccagaa ccggaactt ccttgctat gaaggagct cccaagaaa agaattcca attcgagga ttgcaattt tttatttcg gctccagct tggtattgc aaactttgc gatacatgg attggtgat ttacattgt gcttcacat catttaaaa tcacaagcc cattttcaa aattatgcg aatttcgtc gaacaaaat ccacattgg gaaattgag tggttaaaa gatcgcgaa aaagcaatg gctcgtaac gagagacgt ggtcgttct tccgattca gattccgat aaaaagaag aagaaacat cgaaataaa agatcatca atggaatcg agtagtaaa gacaagaaa aagaagaaa cgttcaaag aaacgtagg aagcattcc agtgatacg agtagtagt tcaagttca tccgattcc tcatcggaa gaagaagaa aataacgat aaatcgaaa agtattcga gttgtgatg cgtaacatg aaagtccaa tcaataatg aatgaggat ttaagcggt aaatgggaa atgttggag cgtttagtc gatcaacat aaaaaaaag gaagaaatg caaaaaagt aaagaagca caaaagaga gaacaaccg gaagataat ttgataaat caatggatg acagtgagc gaacctcaa gaaaaagaa aaagtctta cttgataat ttaaaagat agaatgaag caaaagcaa gaattggaa agagcaaaa tatgccgaa atggaaaag aaacaacgt gagaaggaa cgagaagaa cgcgaatta aaagagaga aaatcaagg gaagaacaa gaagctaaa gaacgctac gaaagggaa cggcgtgaa agagatcga gaggagtat gagagaata agggacaaa gatcgcaat caagtgagg ttcaaaccg aaagatcgg gagaattac aggaagagg aagacgccg tctcctagc cctgaaagg cccccagaa aaaccccca caaccacca caacaaaga caatcgaag gagaggaga gagacacca agcaaggag gagaccagg gaaaggaag aacagtcgt gagaacgga cgagagagt caagagcgc cccagaagt cgcagtcga agcgaagag cgtcgccag aatgagtca aagaagccc tcaggtccg cccacgaaa cgtttaccg ttcattggc cgaatgcca cttttcaaa aacaagaaa gtggaggaa aaagtggag aaagaaatc aagaaggag gattacgat cttccgaga gttacccgt ttccagcct ggaaaccta gcacgtgca ttccttcca caacccgat gttgtgtgt tttcccaaa ctttcgagt tatcccccg atttcggca ccacctcca ccgagtgtc tcacagcct gatcctcca gcacctccg aaaattagc gaagataaa ccaccgaaa gcaccacca gctccgaaa attggatcg agtgaaatt gacgataaa aagagtccg gagaaagaa gaggagatg tatgaggat ccgagtcag atgatgatg aatcaatat tattccgat tatagtgcg atgtattcg caaatgtat gactattca cagtgtcag gaggagccg ccacaaccg ccgccccct gatgatatt ccattggcg cataccatg cccttgcaa ccgcccccg ttgccgcca aatgacccc agtgaggat ttggcgatg ttgggaatt agtgcagac gatatggct gctcaaagt ttctaagtt tttgatatt agacaatta ttttgctgt agtttggcc gattggtcg ctattttta agaccgtaa tatgttatt tgccttaat aaatgcgtt tcttttgt
