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Gene information
General information
TC030712 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3282608..3285464
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030712
    iBeetle screen iB_02616

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 3
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature potentially degenerated - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larva cuticle thinner - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 3
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030712 RA
      MCHKLLIFA LVCLICCEF SDESLQGPT KSKRDEEEK 
      SDDSDETSP LRVVPAPDL SKDRDRRKG DTNVNDEIS 
      DAVDLFMLD QAKVIQKMV QEIGLKNFM LEEPEELPL 
      EDLPPPPEL PAHEPTPEE IEAKTIYDT ATAILNKTR 
      PDKKHAYQL LAEAAKKGN PEAKALVAW AKLFGNPLE 
      QDLETAKEI FRSLAEGGH PEGHTGLGF LYASGLSVN 
      VSQAKALVH YTFGAIGGN TWAQMVLGY KYWSGITVA 
      PSCEKALDF YRQVADKVS QGVSFGGGA AIQRIRLLD 
      ELENGYSSG ILDNDLIEY YQLLAEKGD IQAQVGLGQ 
      LHYQGGRGV DLDYQKAMH YFTQAANAG NAIAMAYLG 
      KIYLDGSDE VKADNDTAF KYFKKASDL NNPVGLSGL 
      GLMYLYGRG VEKDYTKAY KYFLAAADQ GWVDGQLQL 
      GNMYFSGLG VRKDYKLAN KYFSLASQS GHVLAYYNL 
      GQMHAQGTG MLRSCPTAV ELFKNVAER GRWGEILMQ 
      AHSDYTEGR FNEAFVQYA LLAELGYEV AQSNAAFLL 
      DRNEVPMFT IQESLARAL LYWGRAAAQ GYSAAQVKL 
      GDYHYYGLG TPVDYETAA SHYRLASEQ QHNAQAMFN 
      LGYMHEQGL GMAKDVHLA KRCYDLAAE TSTDAKVPV 
      ALALFKLNL MFSLDSLKE SHLSSLLDF NEMFGSNWD 
      LYLITTLTA ILAAIVYFR RPQAPAVIH IFL
    CDS sequence
    • >TC030712 RA
      atgtgccac aaattgtta attttcgcg ttggtttgc 
      ctcatctgc tgcgaattt tccgacgag agtttgcag 
      gggcctacc aagagcaag cgcgacgaa gaggagaag 
      tccgatgat tcggacgag acttcgcct cttagggtg 
      gtcccggcg cccgacttg tccaaagac cgcgaccgc 
      cggaaaggt gacacgaat gttaacgat gaaatttca 
      gacgccgtt gatcttttc atgctcgac caagccaaa 
      gtcattcaa aagatggtg caagagatc ggcttgaag 
      aacttcatg cttgaagaa ccggaagaa ctccccctt 
      gaagacctg cccccgccg ccggagctc cccgcccac 
      gaaccgacc ccagaggaa atcgaggcg aagacgatt 
      tacgacacc gccacggcg attttaaat aaaacccgc 
      cccgataaa aaacacgcc taccaactg ttggcagaa 
      gctgcgaaa aaggggaat cccgaagcg aaggcactc 
      gttgcgtgg gcgaaactc ttcgggaat cccctagag 
      caggatttg gaaacggcc aaagagatt ttccggagt 
      ttggccgag ggggggcac cctgagggg cacacggga 
      cttgggttt ttgtacgcg tcagggttg tcagttaac 
      gtgagtcag gccaaagcc ctcgtacat tacacgttt 
      ggggcgatt gggggcaac acctgggcg cagatggtc 
      ttggggtac aagtactgg tcggggatc actgtggcc 
      cccagttgc gagaaagcg ctagatttt taccgacaa 
      gtggctgat aaagtgagt caaggggtt tctttcggg 
      ggcggagcc gccattcaa agaatccgt cttttggac 
      gaattggag aatggatat tcatcgggc attttagat 
      aatgatcta attgaatat tatcaactt ttggccgaa 
      aaaggagat attcaggcg caagtgggt cttggacag 
      ttgcattat caaggcggc cgtggtgtt gatttggat 
      taccagaag gccatgcat tattttact caagcagcg 
      aatgcggga aatgccatt gctatggcc tacttggga 
      aagatttat ttggacggc agtgacgag gtgaaggcc 
      gacaacgac accgccttc aaatacttt aaaaaagcc 
      tccgatttg aacaatccc gttggctta agtggcctc 
      ggtttaatg tatctttac ggccgagga gtggaaaaa 
      gactacaca aaagcgtac aaatatttc ctagccgcc 
      gctgatcaa ggctgggtc gatggccaa ctccaactc 
      ggaaatatg tattttagc ggtttgggc gttcgaaaa 
      gactacaaa cttgcaaac aaatatttt tcactcgca 
      tcacaatcg ggccatgtc ttggcctac tataacctg 
      ggccaaatg cacgctcaa ggcaccgga atgttacga 
      tcgtgcccg accgcagtc gaactgttc aagaatgtg 
      gcagaaagg gggcgctgg ggcgaaatt ctgatgcaa 
      gcgcattcg gattacact gaggggcgt tttaatgag 
      gcttttgtg cagtacgcc ctcttggct gagttgggg 
      tatgaggtg gctcaaagc aatgctgcg tttttattg 
      gacaggaat gaggtgccc atgtttacg attcaggaa 
      tctttggca cgggcgttg ctctattgg gggcgcgca 
      gctgctcag ggctattca gctgctcaa gtcaaacta 
      ggggattat cattattac ggattgggg actccagtc 
      gattatgaa actgcggct agtcattat agactggcg 
      tccgagcag caacataac gctcaagcc atgtttaat 
      ttggggtac atgcacgaa cagggcttg ggaatggcc 
      aaggatgtg catttggcc aaaaggtgc tacgatttg 
      gctgctgag acaagtact gatgctaag gttccggtg 
      gctttggcg ctttttaaa ctgaatttg atgttcagt 
      ttggatagt ttgaaagag agccaccta tcgagtttg 
      ttggacttt aacgagatg tttgggtcg aattgggat 
      ttgtatttg attaccacg ctcacggct attcttgca 
      gcgattgtt tatttccgg agaccgcaa gcgcccgct 
      gttatacac attttttta taa
    mRNA sequence
    • >TC030712 RA
      tcatcaaca aacttgtca taaaaatgt gtaaacact 
      taaaacttg gataaataa ttaaattag gactgactt 
      tggggtgtt gtgacctta aaaactcgc tcatttgtg 
      tcaatagtg cgtcctagt cgtttaaaa aaagtaaaa 
      tgttgacct aaccccaaa aaccaaaat gtaaaatca 
      atctctgtg acgtttttg ggacacgta tctgataaa 
      caagtggaa ataaatcaa aagtgataa ccatgtgcc 
      acaaattgt taattttcg cgttggttt gcctcatct 
      gctgcgaat tttccgacg agagtttgc aggggccta 
      ccaagagca agcgcgacg aagaggaga agtccgatg 
      attcggacg agacttcgc ctcttaggg tggtcccgg 
      cgcccgact tgtccaaag accgcgacc gccggaaag 
      gtgacacga atgttaacg atgaaattt cagacgccg 
      ttgatcttt tcatgctcg accaagcca aagtcattc 
      aaaagatgg tgcaagaga tcggcttga agaacttca 
      tgcttgaag aaccggaag aactccccc ttgaagacc 
      tgcccccgc cgccggagc tccccgccc acgaaccga 
      ccccagagg aaatcgagg cgaagacga tttacgaca 
      ccgccacgg cgattttaa ataaaaccc gccccgata 
      aaaaacacg cctaccaac tgttggcag aagctgcga 
      aaaagggga atcccgaag cgaaggcac tcgttgcgt 
      gggcgaaac tcttcggga atcccctag agcaggatt 
      tggaaacgg ccaaagaga ttttccgga gtttggccg 
      agggggggc accctgagg ggcacacgg gacttgggt 
      ttttgtacg cgtcagggt tgtcagtta acgtgagtc 
      aggccaaag ccctcgtac attacacgt ttggggcga 
      ttgggggca acacctggg cgcagatgg tcttggggt 
      acaagtact ggtcgggga tcactgtgg cccccagtt 
      gcgagaaag cgctagatt tttaccgac aagtggctg 
      ataaagtga gtcaagggg tttctttcg ggggcggag 
      ccgccattc aaagaatcc gtcttttgg acgaattgg 
      agaatggat attcatcgg gcattttag ataatgatc 
      taattgaat attatcaac ttttggccg aaaaaggag 
      atattcagg cgcaagtgg gtcttggac agttgcatt 
      atcaaggcg gccgtggtg ttgatttgg attaccaga 
      aggccatgc attatttta ctcaagcag cgaatgcgg 
      gaaatgcca ttgctatgg cctacttgg gaaagattt 
      atttggacg gcagtgacg aggtgaagg ccgacaacg 
      acaccgcct tcaaatact ttaaaaaag cctccgatt 
      tgaacaatc ccgttggct taagtggcc tcggtttaa 
      tgtatcttt acggccgag gagtggaaa aagactaca 
      caaaagcgt acaaatatt tcctagccg ccgctgatc 
      aaggctggg tcgatggcc aactccaac tcggaaata 
      tgtatttta gcggtttgg gcgttcgaa aagactaca 
      aacttgcaa acaaatatt tttcactcg catcacaat 
      cgggccatg tcttggcct actataacc tgggccaaa 
      tgcacgctc aaggcaccg gaatgttac gatcgtgcc 
      cgaccgcag tcgaactgt tcaagaatg tggcagaaa 
      gggggcgct ggggcgaaa ttctgatgc aagcgcatt 
      cggattaca ctgaggggc gttttaatg aggcttttg 
      tgcagtacg ccctcttgg ctgagttgg ggtatgagg 
      tggctcaaa gcaatgctg cgtttttat tggacagga 
      atgaggtgc ccatgttta cgattcagg aatctttgg 
      cacgggcgt tgctctatt gggggcgcg cagctgctc 
      agggctatt cagctgctc aagtcaaac taggggatt 
      atcattatt acggattgg ggactccag tcgattatg 
      aaactgcgg ctagtcatt atagactgg cgtccgagc 
      agcaacata acgctcaag ccatgttta atttggggt 
      acatgcacg aacagggct tgggaatgg ccaaggatg 
      tgcatttgg ccaaaaggt gctacgatt tggctgctg 
      agacaagta ctgatgcta aggttccgg tggctttgg 
      cgcttttta aactgaatt tgatgttca gtttggata 
      gtttgaaag agagccacc tatcgagtt tgttggact 
      ttaacgaga tgtttgggt cgaattggg atttgtatt 
      tgattacca cgctcacgg ctattcttg cagcgattg 
      tttatttcc ggagaccgc aagcgcccg ctgttatac 
      acatttttt tataaattg tacggtttt agttgaaaa 
      ataaatgtt acataaaag a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY